##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-33A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 743982 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.58655451341565 40.0 40.0 40.0 40.0 40.0 2 39.35178808089443 40.0 40.0 40.0 40.0 40.0 3 39.35136871590979 40.0 40.0 40.0 40.0 40.0 4 39.24494947458406 40.0 40.0 40.0 40.0 40.0 5 39.27276197542414 40.0 40.0 40.0 40.0 40.0 6 39.42035694411962 40.0 40.0 40.0 40.0 40.0 7 39.44127142860983 40.0 40.0 40.0 40.0 40.0 8 39.44252952356374 40.0 40.0 40.0 40.0 40.0 9 39.4517770591224 40.0 40.0 40.0 40.0 40.0 10-11 39.450129169791744 40.0 40.0 40.0 40.0 40.0 12-13 39.37397141328688 40.0 40.0 40.0 40.0 40.0 14-15 39.41215244454839 40.0 40.0 40.0 40.0 40.0 16-17 39.51500170703055 40.0 40.0 40.0 40.0 40.0 18-19 39.547421846227465 40.0 40.0 40.0 40.0 40.0 20-21 39.53031121720687 40.0 40.0 40.0 40.0 40.0 22-23 39.56506743442718 40.0 40.0 40.0 40.0 40.0 24-25 39.545419109602115 40.0 40.0 40.0 40.0 40.0 26-27 39.521886282195 40.0 40.0 40.0 40.0 40.0 28-29 39.53446991997118 40.0 40.0 40.0 40.0 40.0 30-31 39.51164569958547 40.0 40.0 40.0 40.0 40.0 32-33 39.516610636540115 40.0 40.0 40.0 40.0 40.0 34-35 39.513808527986704 40.0 40.0 40.0 40.0 40.0 36-37 39.491497798988775 40.0 40.0 40.0 40.0 40.0 38-39 39.433521388819294 40.0 40.0 40.0 40.0 40.0 40-41 39.47940315580013 40.0 40.0 40.0 40.0 40.0 42-43 39.459243783399714 40.0 40.0 40.0 40.0 40.0 44-45 39.35813198315371 40.0 40.0 40.0 40.0 40.0 46-47 39.3481691675503 40.0 40.0 40.0 40.0 40.0 48-49 39.42227076211558 40.0 40.0 40.0 40.0 40.0 50-51 39.37876073428172 40.0 40.0 40.0 40.0 40.0 52-53 39.33364448658327 40.0 40.0 40.0 40.0 40.0 54-55 39.25868761049464 40.0 40.0 40.0 40.0 40.0 56-57 39.2767169889585 40.0 40.0 40.0 40.0 40.0 58-59 39.212670772694594 40.0 40.0 40.0 40.0 40.0 60-61 39.13608416642418 40.0 40.0 40.0 40.0 40.0 62-63 39.43835528542107 40.0 40.0 40.0 40.0 40.0 64-65 39.440865179254416 40.0 40.0 40.0 40.0 40.0 66-67 39.4412116306233 40.0 40.0 40.0 40.0 40.0 68-69 39.44924341217021 40.0 40.0 40.0 40.0 40.0 70-71 39.43069760790409 40.0 40.0 40.0 40.0 40.0 72-73 39.44248486338462 40.0 40.0 40.0 40.0 40.0 74-75 39.44407863671697 40.0 40.0 40.0 40.0 40.0 76-77 39.43989297871725 40.0 40.0 40.0 40.0 40.0 78-79 39.43969425817075 40.0 40.0 40.0 40.0 40.0 80-81 39.42626290868894 40.0 40.0 40.0 40.0 40.0 82-83 39.43790996045745 40.0 40.0 40.0 40.0 40.0 84-85 39.41048238809556 40.0 40.0 40.0 40.0 40.0 86-87 39.44112513172125 40.0 40.0 40.0 40.0 40.0 88-89 39.40048476008701 40.0 40.0 40.0 40.0 40.0 90-91 39.521272069120585 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03976862246121016 1101 2 -0.03965552490875979 1101 3 0.03538235405023471 1101 4 0.05925703802881088 1101 5 0.03425791672407286 1101 6 0.0675052013691726 1101 7 -0.022658821636042603 1101 8 -0.06182308215035448 1101 9 0.04411308148107196 1101 10-11 -0.018014161332885692 1101 12-13 -0.00879562361014763 1101 14-15 -0.026983637858599252 1101 16-17 -0.02033850969326778 1101 18-19 -0.05227019894616092 1101 20-21 -0.0020287529332492227 1101 22-23 0.009129449108087329 1101 24-25 -0.02711095001626518 1101 26-27 -0.04424167157115022 1101 28-29 0.028249422209036368 1101 30-31 0.01759837488631888 1101 32-33 0.024870818536214756 1101 34-35 0.013677295877855045 1101 36-37 0.02284332683517931 1101 38-39 -0.10966194258241302 1101 40-41 0.00859217743582974 1101 42-43 0.0378056176374173 1101 44-45 -0.10476079470988253 1101 46-47 0.01400475450307681 1101 48-49 -0.1047066148265543 1101 50-51 0.006911339400765826 1101 52-53 -0.0569397250541428 1101 54-55 -0.08530336982810383 1101 56-57 -0.050272634846045605 1101 58-59 -0.1423197535922469 1101 60-61 -0.07169985251075417 1101 62-63 0.0019592833587296354 1101 64-65 0.07073273154770021 1101 66-67 0.03795863084350515 1101 68-69 0.22843518777852978 1101 70-71 0.1299675009796104 1101 72-73 0.08009814689437178 1101 74-75 -0.17354409232027734 1101 76-77 0.10710444361171056 1101 78-79 0.0774860524490677 1101 80-81 -0.001209312959986164 1101 82-83 0.19723747388580648 1101 84-85 -0.09501274313070951 1101 86-87 -0.47067965320690774 1101 88-89 0.07041845014043702 1101 90-91 -1.5642140406770864 1102 1 -0.014350082612608617 1102 2 0.005408321385928616 1102 3 -0.004375406731170983 1102 4 0.03838344197736632 1102 5 0.04059472782092399 1102 6 -0.03187283243759964 1102 7 -0.1320307781837613 1102 8 0.0030181620252136554 1102 9 0.028062009121939013 1102 10-11 -0.06147673521461172 1102 12-13 -0.08368784520960304 1102 14-15 -0.17541934576573937 1102 16-17 -0.04701625058036285 1102 18-19 -0.07799718433805936 1102 20-21 0.012165236262376311 1102 22-23 -0.03366904567714357 1102 24-25 0.041354201848562866 1102 26-27 -0.0584192926200231 1102 28-29 0.036809027633815106 1102 30-31 -0.07871694483386449 1102 32-33 -2.061603648044752E-4 1102 34-35 -0.0302603382568023 1102 36-37 -0.13094837534034554 1102 38-39 0.08560476987694443 1102 40-41 -0.04915225228691611 1102 42-43 -0.1156034708996998 1102 44-45 0.001339729496599773 1102 46-47 -0.07251505706067718 1102 48-49 -0.12759240985811715 1102 50-51 0.05637459504712439 1102 52-53 -0.06287395498376469 1102 54-55 -0.03294923358278368 1102 56-57 0.052011046804508965 1102 58-59 -0.028241570125196347 1102 60-61 -0.07079540877301582 1102 62-63 -0.05678602584463022 1102 64-65 -0.09507628435387971 1102 66-67 0.004753518528531231 1102 68-69 0.02182089695634204 1102 70-71 -0.20862188258195857 1102 72-73 0.11695069248843026 1102 74-75 -0.32446743218122265 1102 76-77 -0.19540007042109409 1102 78-79 -0.27656459663794664 1102 80-81 -0.12062155652770912 1102 82-83 -0.1844735240391131 1102 84-85 -0.2960636283815177 1102 86-87 0.18406346874188984 1102 88-89 0.5878787676007491 1102 90-91 0.8148709266431808 1103 1 0.03975733292048034 1103 2 0.11662276509273539 1103 3 0.04647715020698939 1103 4 0.010726124796690328 1103 5 -0.02387945487798504 1103 6 0.0016655406010528395 1103 7 0.047357969203808636 1103 8 0.005601818295886574 1103 9 0.08738495032107352 1103 10-11 -0.03871588625710842 1103 12-13 0.021284416881670154 1103 14-15 0.0492278180617447 1103 16-17 -0.05694484552464729 1103 18-19 0.009570612414322 1103 20-21 -0.040243848319278186 1103 22-23 -0.025704899225523548 1103 24-25 0.07895240359354716 1103 26-27 0.04959228703810936 1103 28-29 0.034318961546389914 1103 30-31 -0.013770084441823371 1103 32-33 -0.0015824128998787046 1103 34-35 -0.02627840936645498 1103 36-37 -0.04220806006893696 1103 38-39 0.11653565361528706 1103 40-41 0.0208829537498616 1103 42-43 -0.04746062029255427 1103 44-45 0.040875312181150036 1103 46-47 -0.020467137391214862 1103 48-49 -0.05381228686842121 1103 50-51 0.037488997893639464 1103 52-53 0.11077564415435859 1103 54-55 -0.1544752234191904 1103 56-57 -0.12507171053714927 1103 58-59 0.019130738879375997 1103 60-61 -5.91492384536707E-4 1103 62-63 -0.00996421209649867 1103 64-65 -0.07903059685004621 1103 66-67 -0.14744538821190645 1103 68-69 0.004151089708926747 1103 70-71 0.0769360168151465 1103 72-73 -0.12056681355021936 1103 74-75 0.025581780788655806 1103 76-77 -0.33908000629185153 1103 78-79 0.06123646573939112 1103 80-81 -0.11192676903851861 1103 82-83 -0.4582151799196268 1103 84-85 -0.11179803235694408 1103 86-87 0.008496217942372652 1103 88-89 -0.9145930767754393 1103 90-91 0.8148709266431808 1104 1 0.023411082349525714 1104 2 0.05952688380688187 1104 3 -0.0733517152057388 1104 4 -0.09730390702271308 1104 5 -0.03314467257690268 1104 6 -0.04284985963469268 1104 7 -1.9160933707951244E-4 1104 8 0.057973160520297995 1104 9 0.0895065648466229 1104 10-11 -0.004498595002466743 1104 12-13 -0.11470010033791311 1104 14-15 0.07013014919051841 1104 16-17 0.04596452909481741 1104 18-19 -0.01567169107763533 1104 20-21 -0.045549809873200786 1104 22-23 0.005092684405703096 1104 24-25 3.5447890605411203E-4 1104 26-27 0.08277331784050546 1104 28-29 -0.0035399212464426455 1104 30-31 0.0066985863676407575 1104 32-33 0.04030674586481098 1104 34-35 -0.019456353802674187 1104 36-37 -0.0379889436577443 1104 38-39 0.0065295760166392824 1104 40-41 -0.06711075320163928 1104 42-43 0.012031088000135526 1104 44-45 -0.08459810160730541 1104 46-47 -0.07405771597156274 1104 48-49 0.021768742759135762 1104 50-51 -0.05316986985850036 1104 52-53 0.06110002674254389 1104 54-55 0.05017228165861809 1104 56-57 -0.07281545087845842 1104 58-59 0.02095573500744763 1104 60-61 -0.07962555905085367 1104 62-63 0.031236733388809057 1104 64-65 -0.04465416179650106 1104 66-67 0.06690654918113381 1104 68-69 -0.18302028395348202 1104 70-71 -0.05738424241007323 1104 72-73 0.15483217379613023 1104 74-75 0.20525764185683215 1104 76-77 -0.17482881293703656 1104 78-79 0.19330686974090128 1104 80-81 -0.2946986010123851 1104 82-83 -0.15903492754788573 1104 84-85 -0.629868827609215 1104 86-87 0.01720940783099678 1104 88-89 -0.3305120369969501 1104 90-91 0.5185746303468832 1201 1 -4.6210453694328635E-4 1201 2 0.03169569026894692 1201 3 0.12463640703857948 1201 4 -0.031114230901224005 1201 5 -0.0923261179497814 1201 6 0.04128439879008283 1201 7 0.0015137517166223802 1201 8 0.0713620192639155 1201 9 -0.057606509183415255 1201 10-11 -0.07703994720621665 1201 12-13 0.03882068573453523 1201 14-15 -0.06221838327437723 1201 16-17 -0.020031822964178048 1201 18-19 0.01812079468689376 1201 20-21 -0.01842280156773768 1201 22-23 0.026916823016250646 1201 24-25 -0.024508676609613644 1201 26-27 -0.027440076435048866 1201 28-29 0.04139998485596408 1201 30-31 0.020088379043926352 1201 32-33 -0.014614835420772465 1201 34-35 -0.02954415884311601 1201 36-37 0.0318039444452225 1201 38-39 0.028046378637419878 1201 40-41 -0.009090679324955886 1201 42-43 0.06770241905423546 1201 44-45 0.007187836551771909 1201 46-47 0.011707581530323807 1201 48-49 0.07425727843359198 1201 50-51 -0.01797615278737652 1201 52-53 0.05959583440999694 1201 54-55 0.1294472565754674 1201 56-57 -0.006323804437734282 1201 58-59 0.03948919106991866 1201 60-61 0.0979495210504453 1201 62-63 0.03345708067881503 1201 64-65 -0.07765432100856628 1201 66-67 0.0010343942187844846 1201 68-69 0.09986280162461014 1201 70-71 -0.01941928680904681 1201 72-73 -0.0808036762251021 1201 74-75 -0.12741575558564477 1201 76-77 -0.22279674840883956 1201 78-79 -0.08804549080235091 1201 80-81 -0.3514684258277896 1201 82-83 -0.2090447322933855 1201 84-85 0.06471951368558848 1201 86-87 0.18738358385402165 1201 88-89 -0.3671699375548698 1201 90-91 -2.426508383701645 1202 1 -0.005604219907652919 1202 2 -0.1526535461143439 1202 3 -0.12196511359431383 1202 4 -0.11539337610312117 1202 5 -0.06657153766394686 1202 6 -0.002234995722687927 1202 7 -0.023059022102593474 1202 8 0.014679312298490288 1202 9 -0.044455769139979395 1202 10-11 0.03858535728181778 1202 12-13 -0.06284924452002372 1202 14-15 -0.002703741132300763 1202 16-17 -0.01695786891004758 1202 18-19 -0.08869439404068657 1202 20-21 -0.09123590956158978 1202 22-23 -0.06747368230030304 1202 24-25 -0.09537306117447031 1202 26-27 -0.011092801347594161 1202 28-29 -0.002713455992420677 1202 30-31 0.021383337193526586 1202 32-33 -0.0039096112110783565 1202 34-35 -0.046527782947926255 1202 36-37 -0.11448226088116797 1202 38-39 -0.06496768609967774 1202 40-41 -0.19794338775305675 1202 42-43 -0.03812211338827609 1202 44-45 -0.08880322206830016 1202 46-47 -0.09356406386204696 1202 48-49 -0.06352019051797697 1202 50-51 -0.16219439063628016 1202 52-53 0.011440011569504804 1202 54-55 -0.014109040283202035 1202 56-57 -0.025387474951109823 1202 58-59 -0.022141736627133923 1202 60-61 -0.12335268145148603 1202 62-63 -0.10644713465435984 1202 64-65 -0.05500726978097248 1202 66-67 -0.1480790434683712 1202 68-69 -0.2107835609599178 1202 70-71 -0.15318544401916512 1202 72-73 -0.075835803397311 1202 74-75 -0.06450750757372248 1202 76-77 0.14343445645088337 1202 78-79 0.2414648728923865 1202 80-81 0.18912429052430468 1202 82-83 0.16005747430313733 1202 84-85 0.267950219832251 1202 86-87 0.3889900640061512 1202 88-89 -0.05974028001830334 1202 90-91 -0.3338470220747709 1203 1 0.09494540427684228 1203 2 0.1589908750843989 1203 3 0.07161244465398653 1203 4 -0.06645701129507131 1203 5 0.050778617408340665 1203 6 0.05465439384289539 1203 7 0.03806511923388456 1203 8 0.04294623358669725 1203 9 -0.030060794328846896 1203 10-11 0.012090089214595423 1203 12-13 -0.013039918288583863 1203 14-15 -0.055263878279866674 1203 16-17 0.05351107651653564 1203 18-19 -0.04454668578663501 1203 20-21 0.030125844763389864 1203 22-23 0.007704857257692765 1203 24-25 -0.008839941387641659 1203 26-27 -0.050694654215227786 1203 28-29 -0.06297913848006687 1203 30-31 0.050780523647276254 1203 32-33 -0.025050019932884027 1203 34-35 -0.011861105098915914 1203 36-37 -0.0409444662210916 1203 38-39 0.048058868757976825 1203 40-41 -0.04092470625548117 1203 42-43 0.020674720861983076 1203 44-45 0.08858917826547952 1203 46-47 0.07278622337248919 1203 48-49 -0.005496917442286531 1203 50-51 -0.05902749888416281 1203 52-53 0.07999493585654704 1203 54-55 -0.1418835449117779 1203 56-57 -0.06030372430939934 1203 58-59 -0.22126083417096254 1203 60-61 0.0012239018711497351 1203 62-63 -0.12650267773445023 1203 64-65 -0.04956145768983333 1203 66-67 -0.07891044793155544 1203 68-69 -0.05119010757267972 1203 70-71 -0.18413889548644136 1203 72-73 -0.1388870544611578 1203 74-75 -0.2961356252144469 1203 76-77 -0.16469876111879245 1203 78-79 0.07195511217231854 1203 80-81 -0.05453741260933498 1203 82-83 -0.49587703281104467 1203 84-85 -0.3882765826632095 1203 86-87 -0.32223990145748616 1203 88-89 -0.28016816131574274 1203 90-91 0.08759819937045421 1204 1 -0.003211542000123302 1204 2 0.023915164114448828 1204 3 -0.2061839277245241 1204 4 0.02885023643822393 1204 5 -0.2881624385914705 1204 6 -0.09276404286121931 1204 7 -0.1675995642481567 1204 8 -0.09043855678142165 1204 9 -0.17108954047640879 1204 10-11 -0.12474268719249437 1204 12-13 -0.12442334497897889 1204 14-15 -0.13426319146770282 1204 16-17 -0.04547939415122215 1204 18-19 -0.11896062016598563 1204 20-21 -0.09028908725910867 1204 22-23 -0.009573367315468317 1204 24-25 -0.06307621969129684 1204 26-27 -0.012839417906583606 1204 28-29 -0.029019982169877778 1204 30-31 -0.07057336663883973 1204 32-33 -0.2038300803589621 1204 34-35 0.003921170353912373 1204 36-37 -0.07386566885892165 1204 38-39 -0.07328647487853601 1204 40-41 -0.07056561341113365 1204 42-43 -0.038397561317843554 1204 44-45 -0.166534076748043 1204 46-47 -0.09514027975714612 1204 48-49 -0.1563174605015618 1204 50-51 -0.17405870726066297 1204 52-53 -0.13543856617956607 1204 54-55 -0.18754346216840645 1204 56-57 -0.19617818697553702 1204 58-59 -0.18593948789534664 1204 60-61 -0.04840392234450519 1204 62-63 -0.1321674123543204 1204 64-65 -0.03858676670222394 1204 66-67 -0.12294717536035193 1204 68-69 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0.03247252984181159 2401 32-33 0.04462373628626182 2401 34-35 -0.00454717987103237 2401 36-37 0.032833158273895435 2401 38-39 0.06394828675387032 2401 40-41 -0.057326940095705936 2401 42-43 0.009618055068720821 2401 44-45 0.04375422019715103 2401 46-47 0.08202101843045284 2401 48-49 0.0857349836701431 2401 50-51 0.08624305437258784 2401 52-53 0.04002752040641866 2401 54-55 0.020012761597186568 2401 56-57 0.14131012335394644 2401 58-59 0.06632032444322533 2401 60-61 0.09363105068762678 2401 62-63 0.04437931010365759 2401 64-65 0.09126277407818151 2401 66-67 0.1594713948645392 2401 68-69 -0.06676120901339289 2401 70-71 0.01746649092657293 2401 72-73 0.06219048687513862 2401 74-75 0.2308805906139213 2401 76-77 0.0765170933472703 2401 78-79 0.39626990176619614 2401 80-81 0.2081918414411632 2401 82-83 -0.11948373540202084 2401 84-85 0.11424651612854575 2401 86-87 0.24881474402940285 2401 88-89 -0.12291685499612015 2401 90-91 -0.4851290733568163 2402 1 -0.02979547673918148 2402 2 0.0036944821689886 2402 3 0.042639503218453 2402 4 0.0073287031546200865 2402 5 -0.04407601549517892 2402 6 0.08334620917059254 2402 7 -0.021029990213698113 2402 8 -0.08063553199474427 2402 9 0.026606600463857433 2402 10-11 0.0037071629637495107 2402 12-13 -0.006326796356674436 2402 14-15 0.0614440714261022 2402 16-17 -0.021199246162204588 2402 18-19 0.029552999906606203 2402 20-21 0.02904901875484711 2402 22-23 -0.023741005041017615 2402 24-25 0.03433975735183026 2402 26-27 0.002636663674650208 2402 28-29 0.007368785098130104 2402 30-31 -0.0012648895206979205 2402 32-33 0.06704207357341829 2402 34-35 0.051395392775184234 2402 36-37 0.032296965658666466 2402 38-39 -0.2967052255228211 2402 40-41 0.09909361926619908 2402 42-43 0.08105392564235814 2402 44-45 0.10467735471392103 2402 46-47 0.11841538380370764 2402 48-49 0.020566431951422715 2402 50-51 0.046424474846539 2402 52-53 0.0022342798052079615 2402 54-55 0.05076217287309248 2402 56-57 0.033831611889738156 2402 58-59 0.030673618337189623 2402 60-61 0.06675341419219905 2402 62-63 0.03654186560506645 2402 64-65 0.09318313145062973 2402 66-67 0.08032627512818635 2402 68-69 -0.16027227039737824 2402 70-71 0.0421014568775675 2402 72-73 -0.07680141902084614 2402 74-75 0.1062723779011705 2402 76-77 0.1265743866153386 2402 78-79 -0.053210063341289526 2402 80-81 -0.03523508795127839 2402 82-83 0.16425274368499032 2402 84-85 0.40509778965098064 2402 86-87 0.3330009227138859 2402 88-89 -0.03247210959223423 2402 90-91 0.2963524081246618 2403 1 0.013294856952271061 2403 2 0.017530282578306355 2403 3 0.1810520106429081 2403 4 0.065733434770479 2403 5 0.13588360272715505 2403 6 -0.013204106293393636 2403 7 0.12498443422268224 2403 8 0.03553183008684613 2403 9 -0.025499622376131015 2403 10-11 0.07095155629041017 2403 12-13 0.061969511263242794 2403 14-15 0.004212718670295601 2403 16-17 0.016057414307084628 2403 18-19 0.005369871828058592 2403 20-21 0.0242605117944521 2403 22-23 -5.124638784792523E-4 2403 24-25 -0.013623174153970297 2403 26-27 -0.020666105834273196 2403 28-29 -0.0750835948871611 2403 30-31 0.017329311246996326 2403 32-33 -0.014582570318758314 2403 34-35 0.0012657571831624637 2403 36-37 -0.0033133727923271294 2403 38-39 -0.0072364313390949064 2403 40-41 -0.01699863364129328 2403 42-43 -0.019198652031313657 2403 44-45 -0.005471761275359199 2403 46-47 0.05772405006442227 2403 48-49 0.03426365026774647 2403 50-51 0.03599694539706633 2403 52-53 0.028806588736557615 2403 54-55 0.016461602623493832 2403 56-57 -0.13179765779276664 2403 58-59 0.030292080047075842 2403 60-61 0.021586674181769183 2403 62-63 0.1073484853249056 2403 64-65 0.05300351715455065 2403 66-67 0.11740881089696131 2403 68-69 0.08290250040715819 2403 70-71 -0.001408338376570839 2403 72-73 0.08401258888264351 2403 74-75 0.019450537028454562 2403 76-77 -0.011182467794604634 2403 78-79 0.0911253399678813 2403 80-81 0.07707729292521037 2403 82-83 -0.34405991049310813 2403 84-85 0.19997084805540766 2403 86-87 -0.02757721626348797 2403 88-89 -0.17575759603561636 2403 90-91 0.2631467887121488 2404 1 -0.07765983125290177 2404 2 -0.09312243753357308 2404 3 -0.006368420780049178 2404 4 0.16748276467158263 2404 5 0.15615833679905933 2404 6 -0.09890370612530575 2404 7 0.0572278885494768 2404 8 0.010641406088993222 2404 9 0.07619322905596704 2404 10-11 0.02614029541636853 2404 12-13 0.020582710955046934 2404 14-15 0.06597529251330769 2404 16-17 0.02190604475602953 2404 18-19 0.038182566944328755 2404 20-21 0.05058103280573789 2404 22-23 -0.05653392362410159 2404 24-25 -0.07936877543981069 2404 26-27 0.041402695304007864 2404 28-29 0.001744740101663922 2404 30-31 0.011769290956202383 2404 32-33 -0.058923238169448666 2404 34-35 -0.02798414615045175 2404 36-37 0.077706549265109 2404 38-39 0.16706687219027572 2404 40-41 0.0013875315281381972 2404 42-43 0.08744512225113965 2404 44-45 0.1027712758393804 2404 46-47 0.09722906308998347 2404 48-49 0.04811846923425378 2404 50-51 -0.032575322687300456 2404 52-53 -0.008123756304989627 2404 54-55 0.05963916109200795 2404 56-57 -0.05935558642533323 2404 58-59 0.16399177222590566 2404 60-61 0.11539062871895567 2404 62-63 0.014996696568886136 2404 64-65 0.10909858212018264 2404 66-67 0.06403738144558702 2404 68-69 0.10885218698630439 2404 70-71 0.07768690793807309 2404 72-73 0.17772110301737598 2404 74-75 -0.04391129888124823 2404 76-77 0.13604626866589342 2404 78-79 -0.0410891906422961 2404 80-81 -0.23769792881999763 2404 82-83 -0.009908249849779338 2404 84-85 -0.4514788437828656 2404 86-87 -0.0016165465641577725 2404 88-89 0.24958783478926705 2404 90-91 0.8148709266431808 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 22.0 31 391.0 32 2621.0 33 4830.0 34 6373.0 35 8093.0 36 11764.0 37 17753.0 38 46787.0 39 317619.0 40 327729.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.583645840894 11.575683282660064 26.93640437537467 31.90426650107126 2 30.770264874754826 16.262043580722743 24.80033232015121 28.167359224371218 3 28.621564260377617 18.690934311494516 19.910320290437525 32.77718113769034 4 27.851345865894604 20.013925068079605 19.239040729480013 32.895688336545774 5 28.39678916963045 20.244979045191954 19.100193284246124 32.25803850093148 6 28.8036538518405 22.957141436217544 22.115051170592835 26.12415354134912 7 27.292595788607787 22.183466804304405 21.93735869953843 28.586578707549375 8 26.508705855786836 22.980260275114183 22.04892053840012 28.462113330698863 9 26.36663252605574 23.12824772642349 22.70659774026791 27.798522007252863 10-11 26.543585731912867 21.8331895126495 22.251815232088948 29.371409523348685 12-13 27.01523692777513 21.735942536244156 22.092604390966446 29.156216145014263 14-15 27.470221053735173 21.490708108529507 21.72391267530666 29.315158162428663 16-17 27.61787247540935 21.250446919414717 21.404415698229258 29.727264906946672 18-19 27.711691949536416 21.59333155909686 21.38049038820832 29.31448610315841 20-21 27.705912239812253 21.646894682935876 21.41059864351557 29.2365944337363 22-23 27.674258248183424 21.152460677812098 21.441311752166047 29.73196932183843 24-25 27.64542690548965 21.54171740714157 21.564231392694985 29.248624294673796 26-27 27.927355769360013 21.613358925350347 21.479619130570363 28.97966617471928 28-29 27.85618469264041 21.31133548929947 21.510466651074893 29.322013166985222 30-31 27.777759109326706 21.50269531362886 21.773066756812884 28.94647882023155 32-33 27.868658795572337 21.590584732626347 21.710426582356277 28.83032988944504 34-35 27.860211046602856 21.17856302257291 21.87801214946986 29.083213781354374 36-37 27.82299172575125 21.51044750256314 21.913329771411245 28.753231000274365 38-39 28.143798638787665 21.49561184188857 21.778499073261727 28.582090446062036 40-41 27.897916830646146 21.286342074316412 21.871446840644676 28.944294254392766 42-43 27.93352295509427 21.55089694686099 22.06447629850483 28.45110379953991 44-45 28.016620751324744 21.546903791221606 22.02283976277217 28.41363569468148 46-47 28.040319329968828 21.188805576783828 22.02141998602886 28.749455107218484 48-49 28.05449631487795 21.545052461537267 22.081317324345104 28.31913389923968 50-51 28.221170896179764 21.53354473098799 21.97460940140629 28.270674971425958 52-53 28.074685913456687 21.22395687404037 22.108666659195087 28.592690553307854 54-55 28.168796548084078 21.62251236823031 22.113593135084912 28.0950979486007 56-57 28.301181337257862 21.45327022261114 22.16618272940615 28.079365710724847 58-59 28.21671511627907 21.24811046511628 22.108430232558142 28.42674418604651 60-61 28.38589326759131 21.541526384760854 22.136765434752274 27.935814912895562 62-63 28.363157246380695 21.518743271543116 22.095404385838062 28.02269509623813 64-65 28.351963979193496 21.339283389448934 22.09338890813933 28.21536372321824 66-67 28.279660940202678 21.44027965188412 22.202349148275268 28.07771025963794 68-69 28.49869886030995 21.368866009692137 22.04303432481295 28.089400805184955 70-71 28.393840560337914 21.105348518063057 22.109049647412476 28.391761274186557 72-73 28.48003323466791 21.428052136885288 22.150906163992314 27.941008464454487 74-75 28.850670969586794 21.295369594967216 21.877897832880574 27.976061602565412 76-77 28.317174311566852 21.272559726293295 22.130343996000274 28.279921966139575 78-79 28.58502595046962 21.296745266358972 21.92650060760615 28.191728175565256 80-81 28.82645617447907 21.180651677827242 21.917623732744755 28.075268414948933 82-83 28.93907156673114 20.820116054158607 21.722437137330754 28.5183752417795 84-85 29.48455223003929 20.325532220380353 21.489825486536226 28.700090063044133 86-87 30.086242923901068 19.93275331484366 20.601420918658107 29.37958284259717 88-89 28.997358958857035 19.88735800426385 21.312883826009482 29.802399210869634 90-91 25.30280534500273 21.28623896225678 23.935297335312963 29.47565835742752 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 2.5 10 4.5 11 5.0 12 5.5 13 8.0 14 16.0 15 27.5 16 36.0 17 48.0 18 80.0 19 128.5 20 175.0 21 227.5 22 299.0 23 411.0 24 566.0 25 728.0 26 946.5 27 1262.5 28 1630.5 29 2019.5 30 2442.0 31 2980.5 32 3569.0 33 4148.5 34 4967.5 35 5974.5 36 7066.5 37 8288.5 38 9634.5 39 11248.0 40 13072.0 41 15069.5 42 17073.0 43 19074.5 44 21289.5 45 23622.0 46 26165.0 47 28900.5 48 31387.5 49 33846.0 50 36564.0 51 39299.0 52 41713.5 53 43949.5 54 46437.5 55 48740.5 56 50658.0 57 52206.0 58 53402.5 59 54537.0 60 55332.5 61 55612.0 62 55406.5 63 54381.5 64 52352.0 65 49425.5 66 45705.0 67 42014.5 68 37959.0 69 33305.5 70 28838.0 71 24583.5 72 20484.0 73 16452.0 74 12892.0 75 10242.5 76 8101.0 77 6077.5 78 4475.5 79 3233.5 80 2299.0 81 1655.0 82 1192.5 83 847.5 84 589.5 85 412.0 86 278.0 87 193.5 88 140.5 89 93.0 90 59.0 91 42.0 92 29.0 93 16.5 94 11.5 95 11.5 96 7.5 97 3.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01626383434007812 3 1.344118540502324E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.06276618585823221 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.2640159567844636E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 5.98623166716552E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 7565.0 32-33 17751.0 34-35 20515.0 36-37 23459.0 38-39 26115.0 40-41 28751.0 42-43 30881.0 44-45 32516.0 46-47 32945.0 48-49 34113.0 50-51 34527.0 52-53 35095.0 54-55 34192.0 56-57 33478.0 58-59 32328.0 60-61 31910.0 62-63 30853.0 64-65 29251.0 66-67 27272.0 68-69 25989.0 70-71 24357.0 72-73 22448.0 74-75 20841.0 76-77 18893.0 78-79 17068.0 80-81 15562.0 82-83 13879.0 84-85 12296.0 86-87 10965.0 88-89 9597.0 90-91 8570.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.87794303954998 #Duplication Level Percentage of deduplicated Percentage of total 1 99.88978525600504 99.76786282032151 2 0.10141793140249443 0.20258828751614652 3 0.006125781028392397 0.018354912258795954 4 0.0021474981881024007 0.008579508068353133 5 5.235333759668277E-4 0.0026144718352059063 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0016129422486027888 0.0 0.0 0.0 0.0 2 0.0016129422486027888 0.0 0.0 0.0 0.0 3 0.0016129422486027888 0.0 0.0 0.0 0.0 4 0.0016129422486027888 0.0 0.0 0.0 0.0 5 0.0016129422486027888 0.0 0.0 0.0 0.0 6 0.0018817659567032536 0.0 0.0 0.0 0.0 7 0.002016177810753486 0.0 0.0 0.0 0.0 8 0.009140006075415803 0.0 1.344118540502324E-4 0.0 0.0 9 0.009274417929466035 0.0 1.344118540502324E-4 0.0 0.0 10-11 0.009408829783516268 0.0 1.344118540502324E-4 0.0 0.0 12-13 0.009408829783516268 0.0 1.344118540502324E-4 0.0 6.72059270251162E-5 14-15 0.009408829783516268 0.0 1.344118540502324E-4 6.72059270251162E-5 2.0161778107534857E-4 16-17 0.009408829783516268 0.0 1.344118540502324E-4 1.344118540502324E-4 2.688237081004648E-4 18-19 0.009408829783516268 0.0 1.344118540502324E-4 1.344118540502324E-4 2.688237081004648E-4 20-21 0.009408829783516268 0.0 1.344118540502324E-4 1.344118540502324E-4 2.688237081004648E-4 22-23 0.009408829783516268 0.0 1.344118540502324E-4 1.344118540502324E-4 2.688237081004648E-4 24-25 0.009744859418641848 0.0 1.344118540502324E-4 1.344118540502324E-4 4.032355621506972E-4 26-27 0.010013683126742313 0.0 1.344118540502324E-4 1.344118540502324E-4 4.032355621506972E-4 28-29 0.010618536469968359 0.0 1.344118540502324E-4 1.344118540502324E-4 4.032355621506972E-4 30-31 0.010887360178068824 6.72059270251162E-5 1.344118540502324E-4 1.344118540502324E-4 4.704414891758134E-4 32-33 0.011088977959144174 1.344118540502324E-4 1.344118540502324E-4 1.344118540502324E-4 5.376474162009296E-4 34-35 0.011828243156420452 1.344118540502324E-4 1.344118540502324E-4 1.344118540502324E-4 5.376474162009296E-4 36-37 0.01216427279154603 1.344118540502324E-4 1.344118540502324E-4 1.344118540502324E-4 5.376474162009296E-4 38-39 0.01236589057262138 1.344118540502324E-4 1.344118540502324E-4 1.344118540502324E-4 5.376474162009296E-4 40-41 0.01236589057262138 1.344118540502324E-4 1.344118540502324E-4 1.344118540502324E-4 6.048533432260457E-4 42-43 0.012500302426671613 1.344118540502324E-4 1.344118540502324E-4 1.344118540502324E-4 6.72059270251162E-4 44-45 0.012500302426671613 2.0161778107534857E-4 1.344118540502324E-4 1.344118540502324E-4 6.72059270251162E-4 46-47 0.012634714280721845 2.688237081004648E-4 1.344118540502324E-4 1.344118540502324E-4 6.72059270251162E-4 48-49 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