##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-32C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 924503 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.56576885093937 40.0 40.0 40.0 40.0 40.0 2 39.362290874123715 40.0 40.0 40.0 40.0 40.0 3 39.28778597797952 40.0 40.0 40.0 40.0 40.0 4 39.16466685343368 40.0 40.0 40.0 40.0 40.0 5 39.223375154001666 40.0 40.0 40.0 40.0 40.0 6 39.33201298427371 40.0 40.0 40.0 40.0 40.0 7 39.35782577233389 40.0 40.0 40.0 40.0 40.0 8 39.38729025216792 40.0 40.0 40.0 40.0 40.0 9 39.38659798832454 40.0 40.0 40.0 40.0 40.0 10-11 39.38432217093941 40.0 40.0 40.0 40.0 40.0 12-13 39.30308933556732 40.0 40.0 40.0 40.0 40.0 14-15 39.33717467655595 40.0 40.0 40.0 40.0 40.0 16-17 39.451653483006545 40.0 40.0 40.0 40.0 40.0 18-19 39.48915904004638 40.0 40.0 40.0 40.0 40.0 20-21 39.46666262846091 40.0 40.0 40.0 40.0 40.0 22-23 39.507164389947896 40.0 40.0 40.0 40.0 40.0 24-25 39.48661929707097 40.0 40.0 40.0 40.0 40.0 26-27 39.45706179428298 40.0 40.0 40.0 40.0 40.0 28-29 39.46777241393484 40.0 40.0 40.0 40.0 40.0 30-31 39.416167423095615 40.0 40.0 40.0 40.0 40.0 32-33 39.41373612085212 40.0 40.0 40.0 40.0 40.0 34-35 39.40441560032778 40.0 40.0 40.0 40.0 40.0 36-37 39.385503167607325 40.0 40.0 40.0 40.0 40.0 38-39 39.318739313854124 40.0 40.0 40.0 40.0 40.0 40-41 39.35634201295677 40.0 40.0 40.0 40.0 40.0 42-43 39.33704083244709 40.0 40.0 40.0 40.0 40.0 44-45 39.25103169609901 40.0 40.0 40.0 40.0 40.0 46-47 39.19550464139124 40.0 40.0 40.0 40.0 40.0 48-49 39.269435182040645 40.0 40.0 40.0 40.0 40.0 50-51 39.196087150543136 40.0 40.0 40.0 40.0 40.0 52-53 39.145190320051725 40.0 40.0 40.0 40.0 40.0 54-55 39.04008760423161 40.0 40.0 40.0 40.0 40.0 56-57 39.069909430650235 40.0 40.0 40.0 40.0 40.0 58-59 39.01870042555391 40.0 40.0 40.0 40.0 40.0 60-61 38.91872338245858 40.0 40.0 40.0 40.0 40.0 62-63 39.39839294490679 40.0 40.0 40.0 40.0 40.0 64-65 39.397596864596984 40.0 40.0 40.0 40.0 40.0 66-67 39.39220428474521 40.0 40.0 40.0 40.0 40.0 68-69 39.391301900633934 40.0 40.0 40.0 40.0 40.0 70-71 39.39560108845342 40.0 40.0 40.0 40.0 40.0 72-73 39.40039186220183 40.0 40.0 40.0 40.0 40.0 74-75 39.40380592412639 40.0 40.0 40.0 40.0 40.0 76-77 39.40745726937503 40.0 40.0 40.0 40.0 40.0 78-79 39.38264191123284 40.0 40.0 40.0 40.0 40.0 80-81 39.38269081038542 40.0 40.0 40.0 40.0 40.0 82-83 39.37451333293906 40.0 40.0 40.0 40.0 40.0 84-85 39.366391955269506 40.0 40.0 40.0 40.0 40.0 86-87 39.34645956030432 40.0 40.0 40.0 40.0 40.0 88-89 39.416985887305934 40.0 40.0 40.0 40.0 40.0 90-91 39.50574499477117 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.015582406908755786 1101 2 0.048855969287629364 1101 3 0.025622739536274253 1101 4 -0.17390220551378377 1101 5 -0.10174555016847364 1101 6 0.11027569239460888 1101 7 -0.005341244817323343 1101 8 -0.08232160732221416 1101 9 -0.02806950359823901 1101 10-11 0.0069318561678812785 1101 12-13 -0.02857634361684802 1101 14-15 -0.019980835282439102 1101 16-17 0.00753185022127667 1101 18-19 -0.07495254633199977 1101 20-21 -0.018011535704715698 1101 22-23 0.06312103070939656 1101 24-25 0.03416566139361521 1101 26-27 0.07147786986624993 1101 28-29 0.04414048682394167 1101 30-31 0.0511794691485008 1101 32-33 -0.005357775094488204 1101 34-35 -0.024254443615497223 1101 36-37 -0.06500054721395543 1101 38-39 -0.06813201647243261 1101 40-41 -0.01573012180755029 1101 42-43 -0.058859362830176565 1101 44-45 0.12555755320097006 1101 46-47 0.06412677870868322 1101 48-49 -5.010376067957623E-4 1101 50-51 0.006969107922337514 1101 52-53 0.11158314148783433 1101 54-55 -0.03248051828187215 1101 56-57 0.10019852826627584 1101 58-59 0.03460211807592373 1101 60-61 0.10776773727066313 1101 62-63 -0.07972444736710571 1101 64-65 -3.750080660225308E-4 1101 66-67 -0.027131721264069597 1101 68-69 -0.025579499555234975 1101 70-71 -0.03558505233328191 1101 72-73 -0.07669779868101756 1101 74-75 -0.043753534940904615 1101 76-77 -0.19663435909102134 1101 78-79 -0.03971171988526123 1101 80-81 -0.2226115158981088 1101 82-83 0.04873212922411341 1101 84-85 -0.3529547463486935 1101 86-87 -0.43873842182472345 1101 88-89 0.4739232014536441 1101 90-91 0.29608670143209537 1102 1 0.08635674831437967 1102 2 0.10257238181993245 1102 3 0.2307535113715673 1102 4 0.07685105139973558 1102 5 -0.08108827904767679 1102 6 -0.06835735801770682 1102 7 -0.034252725082787094 1102 8 -0.11557261008826458 1102 9 -0.021741058111665268 1102 10-11 -0.06533790685504925 1102 12-13 -0.08151577724748904 1102 14-15 -0.0724075462559739 1102 16-17 -0.1028626110631663 1102 18-19 0.010284243797748616 1102 20-21 -0.07528989599322955 1102 22-23 -0.032049351260049264 1102 24-25 -0.006732386789494171 1102 26-27 -0.010108191942599376 1102 28-29 2.3224123962251042E-4 1102 30-31 0.06755799372574955 1102 32-33 -0.005488571230152672 1102 34-35 -0.006591260633221907 1102 36-37 -0.1602238401368723 1102 38-39 -0.02540439543892603 1102 40-41 -0.09461886616216475 1102 42-43 -0.042577812529387415 1102 44-45 -0.03815221956227077 1102 46-47 -0.045354196182302076 1102 48-49 -0.025736683955727813 1102 50-51 0.00322118851408959 1102 52-53 -0.050629537561910354 1102 54-55 -0.10831938575655897 1102 56-57 -0.05641498810162915 1102 58-59 0.11402894515148176 1102 60-61 0.09527817976633912 1102 62-63 0.032610114636078436 1102 64-65 0.05444433753007871 1102 66-67 0.0984657662964139 1102 68-69 0.14776372964070816 1102 70-71 -0.01256566162967232 1102 72-73 0.05140474425496677 1102 74-75 -0.010183127135519499 1102 76-77 0.01430934412849183 1102 78-79 0.15598437146299915 1102 80-81 0.20841685652142417 1102 82-83 0.07890756867877968 1102 84-85 0.25681326341931765 1102 86-87 0.186191559468881 1102 88-89 -0.167985244710124 1102 90-91 -0.992802187456796 1103 1 0.05294688486939236 1103 2 0.15981719605089495 1103 3 0.010252017471806596 1103 4 0.02143598274430758 1103 5 0.06488378782496085 1103 6 0.17091894184950718 1103 7 0.034342674825204256 1103 8 0.14272385724399328 1103 9 0.047496816807750974 1103 10-11 -0.022374643488987545 1103 12-13 0.05905106886157796 1103 14-15 0.01315413606921112 1103 16-17 0.0025280013884909636 1103 18-19 0.0987498550320467 1103 20-21 -0.013754162681408388 1103 22-23 -0.005280528159431697 1103 24-25 0.01253895642661007 1103 26-27 -0.029337672533536363 1103 28-29 0.04842891417840889 1103 30-31 0.06702889665751144 1103 32-33 0.07586497867235664 1103 34-35 0.004269689111040975 1103 36-37 0.055191325559036386 1103 38-39 0.09207798483432583 1103 40-41 0.06090193439791847 1103 42-43 0.07504425067889287 1103 44-45 0.029621176036151553 1103 46-47 -0.046455867934334094 1103 48-49 0.056532390518398756 1103 50-51 0.19660367065834095 1103 52-53 0.08859474378761689 1103 54-55 0.18204123502934522 1103 56-57 0.18928928750495544 1103 58-59 0.07313021777617479 1103 60-61 0.27218477782594164 1103 62-63 0.07631258824090992 1103 64-65 0.14156690072309885 1103 66-67 0.11000278000784647 1103 68-69 0.08938558735570723 1103 70-71 -0.009673040353831652 1103 72-73 0.16821313551622552 1103 74-75 0.11181773856393562 1103 76-77 -0.14218033610251268 1103 78-79 0.26930968337698147 1103 80-81 0.2914393881784463 1103 82-83 -0.06444034900068374 1103 84-85 0.17381925484391303 1103 86-87 -0.7239074894517756 1103 88-89 -0.3712486479735304 1103 90-91 -0.12496593014684976 1104 1 0.06763315169988005 1104 2 0.01837025830550232 1104 3 -0.049551198542374664 1104 4 -0.03703875049166072 1104 5 0.09812074158963213 1104 6 0.06274232546517311 1104 7 0.004952609037310651 1104 8 -0.02291967785490101 1104 9 -0.015376783626592783 1104 10-11 0.006902877355159376 1104 12-13 -7.094479402809384E-4 1104 14-15 -0.02992201698626218 1104 16-17 -0.010973150217743921 1104 18-19 0.011213924775979933 1104 20-21 0.012369271975373408 1104 22-23 -0.09045131793934047 1104 24-25 0.012008750975539328 1104 26-27 0.021195572762131576 1104 28-29 0.043222438036956135 1104 30-31 -0.011912332542522108 1104 32-33 0.03538742972914832 1104 34-35 0.035802832446805155 1104 36-37 -0.1096482380519177 1104 38-39 -0.0651911246336212 1104 40-41 -0.06524365552273537 1104 42-43 -0.02521718331247058 1104 44-45 -0.056161996170779105 1104 46-47 0.03856512624068387 1104 48-49 -0.10973451264575829 1104 50-51 -0.00969164364243369 1104 52-53 0.11514319748911817 1104 54-55 0.010675342478975836 1104 56-57 -0.01769976682470542 1104 58-59 0.03171401738883617 1104 60-61 0.15742764625645123 1104 62-63 -0.15657110349177827 1104 64-65 -0.03320469501287704 1104 66-67 -0.3371685995831015 1104 68-69 -0.20500727950090436 1104 70-71 -0.12048048340665929 1104 72-73 -0.3503396839943065 1104 74-75 -0.08844650253047348 1104 76-77 -0.31055796138843306 1104 78-79 -0.39575867593222114 1104 80-81 -0.011330315873870234 1104 82-83 0.10180319157441176 1104 84-85 -0.2762757716367972 1104 86-87 0.03646737779470044 1104 88-89 0.27204294415527386 1104 90-91 0.04608670143209537 1201 1 0.026411048024662875 1201 2 0.09612709267477015 1201 3 0.0093858220560179 1201 4 -0.03895825560588406 1201 5 -0.06521102872346773 1201 6 0.02917911470468937 1201 7 0.022460498445234123 1201 8 -0.023387740063299134 1201 9 0.06542245509722733 1201 10-11 0.05089678692070265 1201 12-13 -0.018878081415223846 1201 14-15 -0.005242705701540729 1201 16-17 0.050544119561884315 1201 18-19 0.04525560537808815 1201 20-21 0.07562292878169075 1201 22-23 0.0010680643085549946 1201 24-25 0.12274568735648472 1201 26-27 0.11893900097464183 1201 28-29 0.06377160664988679 1201 30-31 0.0795142248683689 1201 32-33 -0.013620342003868302 1201 34-35 0.016606768282976248 1201 36-37 0.045495023432096104 1201 38-39 0.10272144998999266 1201 40-41 0.11942003874861484 1201 42-43 0.051098510098839256 1201 44-45 -0.023385104767776 1201 46-47 -0.0383072028618443 1201 48-49 0.15650358280448984 1201 50-51 -0.09682417915705344 1201 52-53 0.13143487883687754 1201 54-55 0.1133332786600505 1201 56-57 0.0017113692273369452 1201 58-59 0.11273909653534275 1201 60-61 0.03393959805958957 1201 62-63 -0.1461458487650802 1201 64-65 -0.08258952703756961 1201 66-67 0.026864058363379684 1201 68-69 0.10882481591113446 1201 70-71 -0.026825687692202393 1201 72-73 0.22254981798640472 1201 74-75 -0.08170883700383769 1201 76-77 0.0877781153599102 1201 78-79 -0.11724228578792406 1201 80-81 0.06969111738791867 1201 82-83 -0.07488732522411823 1201 84-85 -0.36354105926059077 1201 86-87 -0.14690713707202008 1201 88-89 0.05188790539558852 1201 90-91 -0.31929791395251783 1202 1 -0.015538127282916037 1202 2 0.0782844416696733 1202 3 -0.03396248854749473 1202 4 0.04107013428573225 1202 5 -0.06639526045736943 1202 6 0.15573921559625603 1202 7 0.09787864139569535 1202 8 0.06406217468219921 1202 9 -5.438898738887588E-4 1202 10-11 0.050354438194432305 1202 12-13 0.035522493169033 1202 14-15 -0.0019843718262393395 1202 16-17 -0.060546035310061086 1202 18-19 -0.03351701251489203 1202 20-21 -0.011423193481469696 1202 22-23 0.044107592425575604 1202 24-25 -0.0432721213623779 1202 26-27 0.04015790004630304 1202 28-29 -0.011991513071187399 1202 30-31 -0.01804152988142249 1202 32-33 -0.06410742501618927 1202 34-35 -0.03844088534173551 1202 36-37 -0.18012589428680315 1202 38-39 -0.16024469789240214 1202 40-41 -0.11274711338253951 1202 42-43 -0.23379255690856837 1202 44-45 -0.16631704854597018 1202 46-47 -0.11175136251589635 1202 48-49 -0.09890723617595398 1202 50-51 0.02061645011058033 1202 52-53 -0.09045928031529371 1202 54-55 -0.1873324083928125 1202 56-57 -0.11339149032480833 1202 58-59 -0.21425824964324391 1202 60-61 -0.26564167410696626 1202 62-63 -0.14426504110681293 1202 64-65 -0.002975969934603029 1202 66-67 -0.13274937784580487 1202 68-69 -0.037858129584044775 1202 70-71 -0.24299345268158135 1202 72-73 -0.1443495800012471 1202 74-75 -0.46203103277650825 1202 76-77 0.0029133177974074442 1202 78-79 -0.232034952208501 1202 80-81 -0.25280710136438955 1202 82-83 -0.24476603859855572 1202 84-85 -0.07247189586583858 1202 86-87 0.32006282594711166 1202 88-89 -0.07014974687462683 1202 90-91 0.29608670143209537 1203 1 -0.13767488395365035 1203 2 -0.14958005804973595 1203 3 -0.10662651028025039 1203 4 -0.09920052007017688 1203 5 -0.10646684677556095 1203 6 -0.10669332323318059 1203 7 0.0450438064434735 1203 8 -0.08392606550609116 1203 9 0.023091879832591644 1203 10-11 -0.10025435262778615 1203 12-13 -0.14662113176779457 1203 14-15 -0.09633675854870205 1203 16-17 -0.05751143432561179 1203 18-19 -0.0610182758849831 1203 20-21 -0.015149925834073485 1203 22-23 -0.030682799819416573 1203 24-25 -0.05276512606127426 1203 26-27 -0.08119570677057908 1203 28-29 -0.046149131402195565 1203 30-31 -0.04560365837383529 1203 32-33 0.05906372428312778 1203 34-35 -0.1333796718293101 1203 36-37 -0.02187133183433332 1203 38-39 0.035953247160215085 1203 40-41 -0.07674373383954958 1203 42-43 -0.1449493109094675 1203 44-45 -0.17083181307324224 1203 46-47 -0.11589655405141741 1203 48-49 -0.0916343103565751 1203 50-51 -0.0658001720116701 1203 52-53 0.014322210789082135 1203 54-55 -0.033530033498429646 1203 56-57 -0.08716376424931838 1203 58-59 0.0028610871902117196 1203 60-61 -0.20858241674716993 1203 62-63 -0.13797802124130243 1203 64-65 -0.389744177418784 1203 66-67 -0.12730625157696096 1203 68-69 -0.18302710166891956 1203 70-71 0.046541709224634076 1203 72-73 -0.25777012477800554 1203 74-75 -0.09872941935164903 1203 76-77 0.06008389002687409 1203 78-79 0.3678318508646612 1203 80-81 -0.07813877292460347 1203 82-83 0.07409205491553195 1203 84-85 -0.3467560627306554 1203 86-87 -0.03348720014908224 1203 88-89 -0.11227428899916703 1203 90-91 -0.5610561557107658 1204 1 0.004310397135988353 1204 2 -0.04320392275143803 1204 3 -0.1292958860265685 1204 4 -0.2793460799243803 1204 5 -0.27852155425402003 1204 6 -0.28183002743820396 1204 7 -0.19160466467570103 1204 8 -0.2534152483085492 1204 9 -0.1260789340657169 1204 10-11 -0.07913656536565838 1204 12-13 -0.18135699989635157 1204 14-15 -0.18341280579964803 1204 16-17 -0.07961518899866604 1204 18-19 -0.17509994608281687 1204 20-21 -0.11861622175599962 1204 22-23 -0.0929461937766689 1204 24-25 -0.0710971684407724 1204 26-27 -0.11740007166542199 1204 28-29 0.041734208912011184 1204 30-31 0.010091416744742787 1204 32-33 -0.09713081279081592 1204 34-35 -0.1020575660313554 1204 36-37 -0.018597976469422406 1204 38-39 -0.052094140022838076 1204 40-41 -0.10836870273811883 1204 42-43 -0.1670989973784458 1204 44-45 -0.03554729153156444 1204 46-47 -0.11321960597572911 1204 48-49 -0.08369911949087339 1204 50-51 -0.19553429956187074 1204 52-53 -0.20530974516857015 1204 54-55 -0.35935606173747203 1204 56-57 -0.15699951303638215 1204 58-59 -0.23013107478920602 1204 60-61 -0.21908906343676904 1204 62-63 -0.21359474693613123 1204 64-65 -0.1227190204872528 1204 66-67 -0.10578738829124035 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0.053248069906466355 2401 34-35 0.027179181767884586 2401 36-37 0.06068141757710066 2401 38-39 0.12267756292174425 2401 40-41 0.06783038527682095 2401 42-43 0.22305981796949936 2401 44-45 0.03629538744366556 2401 46-47 0.11280825699662955 2401 48-49 0.020620961463095 2401 50-51 0.15205337034462474 2401 52-53 -0.02878863835605472 2401 54-55 0.07189764441244506 2401 56-57 0.1863471298801045 2401 58-59 0.1404956697596731 2401 60-61 0.02777358831738752 2401 62-63 0.03589213509992817 2401 64-65 0.08884048766112329 2401 66-67 0.14962186149602275 2401 68-69 0.005196329607770167 2401 70-71 0.10478587030073072 2401 72-73 0.1591879728559178 2401 74-75 -0.08121523897748517 2401 76-77 0.19838455486929263 2401 78-79 -0.025545438784824626 2401 80-81 -0.14985411355814904 2401 82-83 0.21244226578914294 2401 84-85 0.05009133038159774 2401 86-87 0.00427811314747828 2401 88-89 -1.00434888107376 2401 90-91 -0.8150244096790189 2402 1 -0.0021529957272079514 2402 2 0.03516985669053696 2402 3 0.14169296653025043 2402 4 0.1060273356946908 2402 5 0.1543246480902667 2402 6 0.10797208454241769 2402 7 -0.06614451910134989 2402 8 0.024782269108619914 2402 9 0.12152085170812654 2402 10-11 0.069930262799204 2402 12-13 -0.008652530298157046 2402 14-15 0.055003276528267975 2402 16-17 0.011177153998197298 2402 18-19 -0.05109464014392273 2402 20-21 0.02737528603003625 2402 22-23 -0.0391270702508848 2402 24-25 -0.03839559134760151 2402 26-27 -0.03582284291120885 2402 28-29 -0.04770905166427042 2402 30-31 -0.008410220458301865 2402 32-33 0.009455945093094442 2402 34-35 -0.036185451840388794 2402 36-37 0.061881977724425496 2402 38-39 -0.26855176782673595 2402 40-41 -0.11226326548208476 2402 42-43 -0.001332240501135118 2402 44-45 0.023603276736686496 2402 46-47 -0.08864653388963717 2402 48-49 -0.016771151161307785 2402 50-51 -0.03736854934747669 2402 52-53 -0.08370976901981209 2402 54-55 0.09779845127768994 2402 56-57 -0.1253064927453238 2402 58-59 0.039444093056602014 2402 60-61 -6.202296974322508E-4 2402 62-63 -7.283564705744539E-4 2402 64-65 0.1584718872775639 2402 66-67 0.1539709628839674 2402 68-69 -0.08554162070430493 2402 70-71 -0.1221469684548282 2402 72-73 0.07599219867213947 2402 74-75 0.006950057062816484 2402 76-77 -0.13373469369460622 2402 78-79 0.1517632274995222 2402 80-81 0.15281673994913803 2402 82-83 -0.2283254379199704 2402 84-85 0.18237658898264186 2402 86-87 -0.25131890177789984 2402 88-89 0.0628155165254185 2402 90-91 0.29608670143209537 2403 1 -0.02302941648152057 2403 2 0.023699809357182744 2403 3 0.008977177691271265 2403 4 0.1463149536512205 2403 5 -0.0030723189572796628 2403 6 -0.018513489336804412 2403 7 0.07252562470763024 2403 8 0.06655621466019568 2403 9 0.009578978272088534 2403 10-11 0.09300527259750879 2403 12-13 0.10616250783986203 2403 14-15 0.03265710992693016 2403 16-17 -0.0041458886018261865 2403 18-19 0.017409083212228893 2403 20-21 0.04330356243270472 2403 22-23 0.045968953144452485 2403 24-25 0.02452083664239524 2403 26-27 0.039869068210627745 2403 28-29 0.01558046565612159 2403 30-31 0.044011468468113435 2403 32-33 0.03920065116545146 2403 34-35 0.0032563300182957278 2403 36-37 0.0019054145737982253 2403 38-39 0.07157415540837064 2403 40-41 0.08189671327262715 2403 42-43 -0.015466445924701588 2403 44-45 0.08733812984035438 2403 46-47 0.020859366816345926 2403 48-49 -0.007414815771483063 2403 50-51 0.15610868529106625 2403 52-53 0.1836255105745579 2403 54-55 0.015609886850135979 2403 56-57 0.17825150054387962 2403 58-59 -0.09792762769973251 2403 60-61 0.11601084915668025 2403 62-63 0.04886611512347372 2403 64-65 -0.02894387273230592 2403 66-67 0.12619170637847077 2403 68-69 0.2792615664416971 2403 70-71 0.043668080296974665 2403 72-73 0.27036613152721145 2403 74-75 0.1362156793088829 2403 76-77 0.2689515870856951 2403 78-79 0.21036203286228528 2403 80-81 0.22321488099436948 2403 82-83 0.29380367735479496 2403 84-85 0.02541198495921293 2403 86-87 0.0036077454470841985 2403 88-89 0.09868142195654173 2403 90-91 0.29608670143209537 2404 1 0.015423338606638026 2404 2 0.06291140385058469 2404 3 -0.0027264883621214153 2404 4 0.028810103521998087 2404 5 0.01572756936447206 2404 6 -0.09465371713180559 2404 7 0.15570695153117242 2404 8 0.028665788206765797 2404 9 0.04548783163711079 2404 10-11 0.04411650700014036 2404 12-13 0.060879840161412346 2404 14-15 0.09896678799289305 2404 16-17 -0.005769160631437842 2404 18-19 0.03166524162353568 2404 20-21 0.04629256642019186 2404 22-23 0.02715796858610986 2404 24-25 0.07216932934392872 2404 26-27 -0.0035091631208530316 2404 28-29 0.0401836857123854 2404 30-31 0.026466349738377914 2404 32-33 -8.587371098016661E-4 2404 34-35 0.07328586301851914 2404 36-37 0.16433730770910415 2404 38-39 0.1640138236970401 2404 40-41 0.13775200635288343 2404 42-43 0.1106454761397373 2404 44-45 0.07847553814220731 2404 46-47 0.0629432393085736 2404 48-49 0.1351252705915016 2404 50-51 0.16622508613684772 2404 52-53 0.17375080487553873 2404 54-55 0.05898633540302711 2404 56-57 0.15624329312481677 2404 58-59 -5.1570221501151536E-5 2404 60-61 -0.08273994879989033 2404 62-63 0.14628147416097903 2404 64-65 0.02407662773760677 2404 66-67 0.25770160450628765 2404 68-69 0.25884086386572136 2404 70-71 0.21788788981498186 2404 72-73 0.43806295784078486 2404 74-75 0.29965722034975073 2404 76-77 0.18458118451566463 2404 78-79 0.22268617207785013 2404 80-81 0.10045577215814916 2404 82-83 0.28349328599177426 2404 84-85 -0.0041553975493471285 2404 86-87 -0.07330481270354738 2404 88-89 0.1860714512820607 2404 90-91 0.29608670143209537 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 36.0 31 630.0 32 3815.0 33 7154.0 34 9599.0 35 12115.0 36 17334.0 37 26156.0 38 70536.0 39 393893.0 40 383235.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.328521378513642 13.801577712565564 28.620458776228958 30.249442132691833 2 28.272574149138833 16.782473141660365 27.50795932872271 27.436993380478093 3 28.24057899217201 19.175708461735656 21.45844848529426 31.125264060798074 4 27.9210559619601 20.8557462766481 20.27197315746947 30.951224603922324 5 27.818514380158852 20.63011153019514 20.694145935708157 30.857228153937843 6 28.739441624310576 22.65087295552313 22.837351528334686 25.77233389183161 7 27.132091512953448 22.55774183534288 22.649899459493373 27.660267192210302 8 26.023928532411468 23.313931918014326 22.95752420489712 27.704615344677087 9 26.4372316801568 23.152547909525442 23.008578663346686 27.401641746971073 10-11 26.102294962807044 22.274724906246924 22.98489025995589 28.63808987099014 12-13 26.922140869202156 22.08548809468439 22.43957023395273 28.55280080216073 14-15 27.037067483826444 21.678674920470783 22.443842799861113 28.840414795841657 16-17 27.04577486498151 21.610908780177024 22.328808019011294 29.01450833583017 18-19 27.261782817362413 21.8085825573308 22.118370627245127 28.81126399806166 20-21 27.218678576489207 21.79089737945685 22.16012279029922 28.83030125375472 22-23 27.02008538641843 21.568561702882523 22.322696627268922 29.088656283430126 24-25 27.311701530443926 21.832703625623715 22.168938337679815 28.68665650625255 26-27 27.232632019582415 21.8221033355219 22.271047254578946 28.67421739031674 28-29 27.244908886179925 21.707284887123134 22.400900808326206 28.646905418370732 30-31 27.57454150148461 21.82723354083625 22.26390909533416 28.334315862344983 32-33 27.513748602378097 21.734117074914757 22.37288718984452 28.379247132862627 34-35 27.26160789740495 21.74751703192974 22.54948883979408 28.441386230871235 36-37 27.52007857637247 21.885784495094125 22.460876826570633 28.133260101962776 38-39 27.8103109228499 21.721780576700503 22.434811395455696 28.0330971049939 40-41 27.365795287061946 21.715950317024895 22.703072943245374 28.215181452667785 42-43 27.687881952009302 21.827598694541134 22.579206654663285 27.90531269878628 44-45 27.71945202202802 21.755930907125272 22.56691375665164 27.957703314195072 46-47 27.444642556885253 21.768450845116945 22.83603886266199 27.950867735335816 48-49 27.756898008302848 21.873553357979223 22.648511047338552 27.72103758637937 50-51 27.812500639541902 21.856881777865098 22.64234156244692 27.68827602014608 52-53 27.6432610141304 21.753650984981427 22.8238815371352 27.779206463752974 54-55 27.90969528526968 21.978451558882426 22.663441798528126 27.448411357319763 56-57 27.945570978709846 21.910374597909765 22.623102014223523 27.52095240915687 58-59 27.69028684245135 21.854723322130905 22.89494908201859 27.56004075339915 60-61 28.161683317631635 22.051095596479474 22.620655316069822 27.16656576981907 62-63 28.04203179463791 21.928242173502298 22.682591132683978 27.347134899175817 64-65 27.75867334687093 21.857696728362967 22.97909444220372 27.40453548256238 66-67 28.112628023065668 21.928253292021687 22.701770806437732 27.257347878474913 68-69 28.170979990507355 21.797481951487597 22.83105593165297 27.200482126352078 70-71 27.98258387804335 21.89351056025752 22.77964518118679 27.344260380512335 72-73 28.58988938281815 21.987318653296118 22.52748199455529 26.895309969330437 74-75 28.147016775175622 22.104970841432937 22.609974595534162 27.138037787857282 76-77 28.16525573960154 21.890414196766788 22.755630749709212 27.18869931392246 78-79 28.656350543894593 21.907461314539606 22.3617282059139 27.074459935651905 80-81 28.633997349059687 21.716579902798856 22.472482086943156 27.1769406611983 82-83 28.440424204616342 21.369931378665 22.671241422333125 27.518402994385525 84-85 29.51718285956725 21.211709800593976 21.615613067458632 27.655494272380142 86-87 29.808645647432087 20.64743208407694 21.621554630180448 27.92236763831053 88-89 29.68490189557699 20.053209178583305 22.06102427668773 28.20086464915198 90-91 27.59949989581163 19.181079391539903 22.17128568451761 31.048135028130865 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 2.0 8 4.5 9 5.0 10 3.5 11 3.0 12 6.5 13 13.5 14 24.5 15 41.0 16 57.0 17 67.0 18 94.0 19 140.5 20 194.0 21 268.0 22 375.0 23 541.5 24 797.5 25 1066.5 26 1390.0 27 1855.0 28 2457.0 29 3206.5 30 4044.5 31 5006.5 32 6110.5 33 7250.5 34 8766.0 35 10614.0 36 12595.0 37 14636.5 38 16975.0 39 19879.5 40 22826.0 41 25851.5 42 28754.0 43 31447.0 44 34455.0 45 37560.5 46 40654.5 47 43858.0 48 46691.0 49 49378.5 50 52318.5 51 55296.0 52 57849.0 53 59997.0 54 62353.0 55 64608.0 56 66407.5 57 68060.0 58 69406.0 59 70041.5 60 70250.5 61 69759.0 62 68632.5 63 66594.5 64 63398.0 65 59498.5 66 54554.5 67 49686.0 68 44733.0 69 39181.0 70 33710.5 71 28502.5 72 23701.5 73 19259.5 74 15340.0 75 12330.5 76 9746.0 77 7321.0 78 5628.5 79 4279.0 80 3105.0 81 2287.5 82 1687.0 83 1202.0 84 884.5 85 663.5 86 466.5 87 317.5 88 214.0 89 140.5 90 92.5 91 67.5 92 48.5 93 31.5 94 22.0 95 14.5 96 8.0 97 4.0 98 4.0 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.012547282161334253 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.05793172870322349 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 4.072042577277188E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 5.142577974338536E-4 76-77 0.0 78-79 7.660428524371653E-4 80-81 0.0 82-83 0.0012476450699305062 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 15627.0 32-33 34553.0 34-35 38297.0 36-37 42110.0 38-39 45931.0 40-41 48991.0 42-43 50865.0 44-45 50036.0 46-47 49115.0 48-49 48393.0 50-51 47752.0 52-53 45314.0 54-55 42932.0 56-57 39585.0 58-59 37044.0 60-61 34170.0 62-63 31871.0 64-65 29197.0 66-67 26440.0 68-69 23828.0 70-71 21351.0 72-73 19414.0 74-75 17335.0 76-77 15523.0 78-79 13606.0 80-81 12318.0 82-83 10945.0 84-85 9573.0 86-87 8436.0 88-89 7436.0 90-91 6515.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.91553277069389 #Duplication Level Percentage of deduplicated Percentage of total 1 99.92334426022053 99.83894177989391 2 0.07035571542352825 0.140592575800103 3 0.005213936662498463 0.015628597783985624 4 5.893027720998765E-4 0.0023552200175042384 5 4.967849213589269E-4 0.0024818265045012224 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0016224933829311532 0.0 1.0816622552874356E-4 0.0 0.0 2 0.001730659608459897 0.0 1.0816622552874356E-4 0.0 1.0816622552874356E-4 3 0.001730659608459897 1.0816622552874356E-4 1.0816622552874356E-4 0.0 1.0816622552874356E-4 4 0.0018388258339886404 2.163324510574871E-4 1.0816622552874356E-4 0.0 1.0816622552874356E-4 5 0.0018388258339886404 2.163324510574871E-4 1.0816622552874356E-4 0.0 1.0816622552874356E-4 6 0.001946992059517384 2.163324510574871E-4 1.0816622552874356E-4 0.0 2.163324510574871E-4 7 0.0022714907361036144 2.163324510574871E-4 1.0816622552874356E-4 0.0 2.163324510574871E-4 8 0.006598139757253357 2.163324510574871E-4 1.0816622552874356E-4 0.0 2.163324510574871E-4 9 0.007138970884897075 2.163324510574871E-4 1.0816622552874356E-4 0.0 3.2449867658623067E-4 10-11 0.007355303335954562 2.163324510574871E-4 1.0816622552874356E-4 0.0 3.2449867658623067E-4 12-13 0.007355303335954562 2.163324510574871E-4 1.0816622552874356E-4 0.0 3.2449867658623067E-4 14-15 0.007355303335954562 2.163324510574871E-4 1.0816622552874356E-4 0.0 3.2449867658623067E-4 16-17 0.007355303335954562 2.163324510574871E-4 1.0816622552874356E-4 0.0 3.2449867658623067E-4 18-19 0.007409386448718934 2.163324510574871E-4 1.0816622552874356E-4 0.0 4.326649021149742E-4 20-21 0.007842051350833906 2.163324510574871E-4 1.0816622552874356E-4 0.0 4.86748014879346E-4 22-23 0.007950217576362651 2.163324510574871E-4 1.0816622552874356E-4 0.0 5.408311276437177E-4 24-25 0.00822063314018451 2.163324510574871E-4 1.0816622552874356E-4 0.0 7.030804659368331E-4 26-27 0.008436965591241997 3.2449867658623067E-4 1.0816622552874356E-4 0.0 7.571635787012048E-4 28-29 0.008815547380592599 3.2449867658623067E-4 1.0816622552874356E-4 0.0 7.571635787012048E-4 30-31 0.00914004605717883 3.2449867658623067E-4 1.0816622552874356E-4 0.0 7.571635787012048E-4 32-33 0.009464544733765061 3.2449867658623067E-4 1.0816622552874356E-4 0.0 7.571635787012048E-4 34-35 0.009843126523115663 3.2449867658623067E-4 1.0816622552874356E-4 0.0 7.571635787012048E-4 36-37 0.010167625199701893 3.2449867658623067E-4 1.0816622552874356E-4 0.0 7.571635787012048E-4 38-39 0.01060029010181687 3.2449867658623067E-4 1.0816622552874356E-4 0.0 9.194129169943202E-4 40-41 0.011032955003931842 3.2449867658623067E-4 1.0816622552874356E-4 0.0 9.73496029758692E-4 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