##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-32B_S36_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1113112
Sequences flagged as poor quality	0
Sequence length	61
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.35849761749042	40.0	40.0	40.0	40.0	40.0
2	39.23276004570969	40.0	40.0	40.0	40.0	40.0
3	39.19386189350218	40.0	40.0	40.0	40.0	40.0
4	39.06395403157993	40.0	40.0	40.0	40.0	40.0
5	39.08142576847613	40.0	40.0	40.0	40.0	40.0
6	39.18341370859356	40.0	40.0	40.0	40.0	40.0
7	39.238811548164065	40.0	40.0	40.0	40.0	40.0
8	39.22989959680607	40.0	40.0	40.0	40.0	40.0
9	39.24383530138926	40.0	40.0	40.0	40.0	40.0
10	39.265364132270605	40.0	40.0	40.0	40.0	40.0
11	39.23950150568856	40.0	40.0	40.0	40.0	40.0
12	39.24965322447337	40.0	40.0	40.0	40.0	40.0
13	39.143230869849575	40.0	40.0	40.0	40.0	40.0
14	39.27310818677725	40.0	40.0	40.0	40.0	40.0
15	39.23232522872811	40.0	40.0	40.0	40.0	40.0
16	39.23511380705625	40.0	40.0	40.0	40.0	40.0
17	39.28200217049138	40.0	40.0	40.0	40.0	40.0
18	39.223380935611154	40.0	40.0	40.0	40.0	40.0
19	39.287676352424555	40.0	40.0	40.0	40.0	40.0
20	38.854692070519405	40.0	40.0	40.0	40.0	40.0
21	39.10301568934663	40.0	40.0	40.0	40.0	40.0
22	39.100945816773155	40.0	40.0	40.0	40.0	40.0
23	39.23995069678523	40.0	40.0	40.0	40.0	40.0
24	39.25430325070613	40.0	40.0	40.0	40.0	40.0
25	38.95813898331884	40.0	40.0	40.0	40.0	40.0
26	39.1974105031659	40.0	40.0	40.0	40.0	40.0
27	39.20597747576165	40.0	40.0	40.0	40.0	40.0
28	38.87805360107518	40.0	40.0	40.0	40.0	40.0
29	39.140812424985086	40.0	40.0	40.0	40.0	40.0
30	38.687682820776345	40.0	40.0	40.0	40.0	40.0
31	38.979056914308714	40.0	40.0	40.0	40.0	40.0
32	38.94649595009307	40.0	40.0	40.0	40.0	40.0
33	39.01102674304113	40.0	40.0	40.0	40.0	40.0
34	39.01730284104385	40.0	40.0	40.0	40.0	40.0
35	38.76352424553863	40.0	40.0	40.0	40.0	40.0
36	38.83913568445943	40.0	40.0	40.0	40.0	40.0
37	38.68806373482632	40.0	40.0	40.0	40.0	40.0
38	38.661374596626395	40.0	40.0	40.0	40.0	40.0
39	38.64744248557198	40.0	40.0	40.0	40.0	40.0
40	38.31507700932161	40.0	40.0	40.0	40.0	40.0
41	38.47047916112664	40.0	40.0	40.0	40.0	40.0
42	38.088849998921944	40.0	40.0	40.0	40.0	40.0
43	38.232059307598874	40.0	40.0	40.0	40.0	40.0
44	37.816183816183816	40.0	40.0	40.0	24.0	40.0
45	38.04137229676798	40.0	40.0	40.0	40.0	40.0
46	37.58789951056138	40.0	40.0	40.0	24.0	40.0
47	36.96440250397085	40.0	40.0	40.0	24.0	40.0
48	37.24519724879437	40.0	40.0	40.0	24.0	40.0
49	36.809866392600206	40.0	40.0	40.0	24.0	40.0
50	36.92929732138365	40.0	40.0	40.0	24.0	40.0
51	36.39820611043633	40.0	40.0	40.0	24.0	40.0
52	36.27911656688635	40.0	40.0	40.0	24.0	40.0
53	35.346910283960646	40.0	40.0	40.0	12.0	40.0
54	34.51319543765587	40.0	40.0	40.0	12.0	40.0
55	34.305755395683455	40.0	24.0	40.0	12.0	40.0
56	34.0168500564184	40.0	24.0	40.0	12.0	40.0
57	34.07537785955052	40.0	24.0	40.0	12.0	40.0
58	33.877086941835145	40.0	24.0	40.0	12.0	40.0
59	33.27475042942669	40.0	24.0	40.0	12.0	40.0
60	32.32597618209129	40.0	24.0	40.0	12.0	40.0
61	32.18051732440222	40.0	24.0	40.0	12.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.03759890773552854
1101	2	0.012790029373981326
1101	3	0.023478868396544783
1101	4	0.05878675495748098
1101	5	0.07353453100644458
1101	6	0.023985201097268316
1101	7	-0.029159466314773397
1101	8	0.05170754085906992
1101	9	0.01928468442979181
1101	10	-0.0241267540754464
1101	11	0.08081423693765544
1101	12	0.010631940439722598
1101	13	0.0028922415370260524
1101	14	0.032381185929757805
1101	15	0.02688829345810717
1101	16	0.11453786610471184
1101	17	0.023237454099813704
1101	18	-0.03106344969537389
1101	19	0.041491127045460985
1101	20	0.16532622473332736
1101	21	0.08985780591898873
1101	22	0.002551725530963722
1101	23	0.018420446284665104
1101	24	0.09856466369479477
1101	25	0.18621646566705152
1101	26	0.1154752477013048
1101	27	0.14698264973606712
1101	28	0.07559458442862166
1101	29	0.04058313521734647
1101	30	0.05517120242332396
1101	31	0.027847804249503838
1101	32	0.027064411821413614
1101	33	-0.013734903536722243
1101	34	0.07766194751929589
1101	35	0.09452223710290042
1101	36	0.0813547026143624
1101	37	-0.007291417781367215
1101	38	0.04510573053972422
1101	39	-0.15737005365407697
1101	40	0.003265890771189106
1101	41	0.09135941375041057
1101	42	0.18892171945136482
1101	43	-0.0020832601083640157
1101	44	0.06917622886041386
1101	45	-0.014730528453029024
1101	46	0.04137680259436394
1101	47	0.01948405042632828
1101	48	0.21454605781390512
1101	49	0.2594879569751214
1101	50	0.02537668669988591
1101	51	0.3050997291409203
1101	52	0.07824740597103386
1101	53	0.12252731564466757
1101	54	0.38065675629334805
1101	55	0.20285617993235405
1101	56	0.3745344224880043
1101	57	0.3882038614206351
1101	58	0.5403387260289207
1101	59	0.4006367428502955
1101	60	-0.2482769650047274
1101	61	0.19160988653633382
1102	1	0.024734689221759254
1102	2	-0.02576206567364636
1102	3	0.036419628487116995
1102	4	-0.06586087575362143
1102	5	-0.11295673115107263
1102	6	-0.051686941890253024
1102	7	-0.08986805813947996
1102	8	-0.19419633992971086
1102	9	-0.03419367229451353
1102	10	-0.1011354307500838
1102	11	-0.03250589460918718
1102	12	0.037865040942733685
1102	13	-0.06765750361876854
1102	14	-0.06713791053945073
1102	15	-0.02646576497910047
1102	16	0.056639723971585454
1102	17	0.09674157327962263
1102	18	0.011617965290163568
1102	19	0.03482481364761725
1102	20	0.12357181328899003
1102	21	-0.027609309006557226
1102	22	0.007659076727925651
1102	23	-0.0487595068875919
1102	24	-0.0902385466028619
1102	25	0.08946333318666433
1102	26	0.007880687779390882
1102	27	-0.03030726816818685
1102	28	0.007269778611984634
1102	29	0.025691546220748762
1102	30	0.0650830095176147
1102	31	0.0076840052751592225
1102	32	0.007484814796463013
1102	33	0.09725496222766594
1102	34	-0.012781411386775687
1102	35	0.012327705049969495
1102	36	-0.04592936018636351
1102	37	0.01260415857343844
1102	38	-0.018333498392209435
1102	39	-0.03538436889154184
1102	40	0.19410105096223873
1102	41	0.049889579963391384
1102	42	0.07732671728922469
1102	43	0.015784291562106034
1102	44	0.03278954081444141
1102	45	0.12503962438807292
1102	46	-0.1576332596790735
1102	47	-0.020380372852514483
1102	48	0.2804725608909422
1102	49	0.256631713177768
1102	50	0.1000858450631057
1102	51	-0.04411500007525859
1102	52	0.01961263293642901
1102	53	0.13047063039213924
1102	54	0.3102430850518516
1102	55	0.1766109237110598
1102	56	0.06165535359993157
1102	57	0.27014403992605907
1102	58	-0.05130345714619011
1102	59	0.1257765947783298
1102	60	-0.1705120313832822
1102	61	-0.07040436066417755
1103	1	0.03436378236589377
1103	2	-0.018385140072005868
1103	3	0.023296557464114187
1103	4	0.044703641645710945
1103	5	0.1085288057607201
1103	6	-0.015558072644871856
1103	7	0.0762484251488047
1103	8	-0.008199961528276845
1103	9	0.050055910045095686
1103	10	0.032085133474780037
1103	11	-0.035308727724086
1103	12	-0.017469616173919178
1103	13	-0.005222057343381437
1103	14	0.08377499525494869
1103	15	0.005865665616624938
1103	16	0.09510209081771848
1103	17	0.027318814171863437
1103	18	0.041348762797284166
1103	19	0.02776613610007672
1103	20	0.008135528960387717
1103	21	0.011231097952340008
1103	22	0.01004272250690974
1103	23	0.03554922458530996
1103	24	0.13213229853217
1103	25	0.08197035955721077
1103	26	-0.07179480207347666
1103	27	0.10504073607897624
1103	28	0.04277211709225526
1103	29	-0.019662016116164693
1103	30	0.051556181367388376
1103	31	0.049198981739273506
1103	32	-0.046725044399671845
1103	33	0.06464482348759049
1103	34	0.08291224239341943
1103	35	0.05864565542962907
1103	36	0.028504680654187098
1103	37	-0.02419238866020379
1103	38	0.008534352478683616
1103	39	0.06962688069497602
1103	40	-0.1704855271350283
1103	41	0.0549063265617562
1103	42	0.071980792905606
1103	43	-0.020374473712593044
1103	44	0.2298217330517005
1103	45	-0.07319676705188982
1103	46	-0.18419154198824828
1103	47	-0.03264160242797942
1103	48	0.3495503905420776
1103	49	0.07652967449359238
1103	50	-0.03676306844125321
1103	51	0.16588274647134682
1103	52	0.011920673907496848
1103	53	-0.2843698871499214
1103	54	-0.24073527054438415
1103	55	-0.03522012450480361
1103	56	0.014032264735753586
1103	57	0.17891936033594646
1103	58	-0.16608331468036397
1103	59	0.03098946575831718
1103	60	-0.20084524995444042
1103	61	0.03979947560716823
1104	1	-0.006890809326677072
1104	2	-0.016124614499808843
1104	3	-0.07729683049858949
1104	4	-0.06493184860577372
1104	5	-0.02936325414321317
1104	6	-0.07659226309415601
1104	7	0.011823446341232113
1104	8	-0.0986209844160939
1104	9	0.04101380775631469
1104	10	-6.924873220555128E-4
1104	11	-0.007052158071637393
1104	12	-0.07398275547880218
1104	13	-0.021045736348753508
1104	14	-0.04959601350459053
1104	15	-0.030302743538499044
1104	16	-0.07330706431084621
1104	17	-0.11170350851816124
1104	18	-0.06150515073761653
1104	19	-0.03439831713529884
1104	20	-0.014469726761568324
1104	21	-0.09049255279646218
1104	22	0.049601920020336365
1104	23	0.05137290359068203
1104	24	0.0371902244999589
1104	25	-0.10678352572111294
1104	26	-0.1328289925227537
1104	27	0.059830224635064155
1104	28	-0.020522598929218816
1104	29	-0.08182646935154025
1104	30	-0.17449637585216493
1104	31	-0.08756281537856125
1104	32	-0.019598737533321753
1104	33	0.025085625974170966
1104	34	-0.0063785177083062194
1104	35	-0.3075594872660474
1104	36	-0.054004502730954584
1104	37	-0.04566738159606132
1104	38	-0.2276905698157492
1104	39	-0.020794142192848142
1104	40	-0.09023686932209074
1104	41	-0.15758307722462916
1104	42	-0.14615992481126483
1104	43	-0.07914813858968017
1104	44	-0.09795447491665499
1104	45	-0.21560987810021004
1104	46	-0.27574282766216385
1104	47	-0.10384815795214308
1104	48	-0.4212888295765964
1104	49	-0.2670702124801281
1104	50	-0.1577011865537159
1104	51	-0.07938427811186699
1104	52	0.0266140901267633
1104	53	-0.4086987936206725
1104	54	-0.11979715243192146
1104	55	-0.1098174683872557
1104	56	0.2310427196665259
1104	57	-0.3059633748999957
1104	58	-0.011237312984967218
1104	59	-0.20862624489441117
1104	60	-0.48680173483717226
1104	61	-0.4891635082865875
1201	1	-0.06589356424230175
1201	2	-0.03624073421334373
1201	3	0.017891759122896644
1201	4	-0.02194637216284434
1201	5	-0.010073649083729208
1201	6	-0.08438675989740574
1201	7	-0.05014309100599945
1201	8	0.029388709945337155
1201	9	-0.09671103080754762
1201	10	-0.10337534269837789
1201	11	-0.04751684475344575
1201	12	-0.12921887368958807
1201	13	-0.012971791264014598
1201	14	-0.08893811103731508
1201	15	-0.06629114195852992
1201	16	-6.968726817433435E-4
1201	17	-0.06285569312668571
1201	18	-0.10317044187819846
1201	19	-0.06124101916677205
1201	20	-0.07009464258136688
1201	21	-0.02712142013965746
1201	22	-0.07243097030701051
1201	23	0.01663626720957012
1201	24	-0.0020712998440544084
1201	25	-0.011881418548384204
1201	26	0.06301309521808918
1201	27	0.03749834223195592
1201	28	0.07889160681138208
1201	29	0.0076161648162056395
1201	30	0.09881331575155627
1201	31	0.04924981958453145
1201	32	-0.02873835895923804
1201	33	-0.013350023185545012
1201	34	-0.013143787946226837
1201	35	-0.03998807747903754
1201	36	-0.09836132205636972
1201	37	-0.10151227501043536
1201	38	-0.09480331992336488
1201	39	0.018401516766743953
1201	40	0.0560140194409513
1201	41	-0.11874296344696944
1201	42	0.09597913348159892
1201	43	-0.0020172155510636003
1201	44	0.10993905976438612
1201	45	0.1666408396046677
1201	46	0.22933180445288315
1201	47	0.839782503503379
1201	48	0.2348279184159665
1201	49	-0.05142118865068568
1201	50	0.05253824291136766
1201	51	0.16315956453380664
1201	52	0.04734506000654193
1201	53	0.02122407433721918
1201	54	0.06174255484280877
1201	55	0.13145095183869415
1201	56	0.37167043116814824
1201	57	0.2980631263251894
1201	58	0.5136567248788353
1201	59	0.3775497779474506
1201	60	0.3741083984802813
1201	61	0.5707150803876857
1202	1	-0.20596633363947348
1202	2	-0.23511015250617362
1202	3	-0.1221586098441847
1202	4	-0.055609098487188646
1202	5	-0.16818068473880743
1202	6	-0.0026853512303546268
1202	7	-0.008054602907030528
1202	8	-0.041668014343763105
1202	9	-0.02633431957469412
1202	10	-0.04318058695906757
1202	11	-0.08348937542603352
1202	12	0.06107245990401822
1202	13	-0.024041763807488792
1202	14	0.007444219503433658
1202	15	0.017657029846368744
1202	16	-0.09324264956065775
1202	17	-0.06829067747838025
1202	18	-0.08256428624001444
1202	19	-0.030486188674025527
1202	20	-0.1828734479344334
1202	21	-0.0031020789683111616
1202	22	-0.05066383237795691
1202	23	-0.07591654980451779
1202	24	0.021892680264357978
1202	25	-0.033081825596198655
1202	26	-0.14605683892464327
1202	27	-0.10029630342523888
1202	28	-0.02601696743324311
1202	29	0.057634725172562185
1202	30	-0.05243406819821672
1202	31	0.07327556087876275
1202	32	0.03831092838708372
1202	33	0.006165231507345936
1202	34	0.01654670211118514
1202	35	0.020324760527451247
1202	36	0.09316715529039499
1202	37	0.06743816883808051
1202	38	0.22573627443684785
1202	39	0.031218722857097703
1202	40	-0.019324067050312976
1202	41	0.00502293285823896
1202	42	-0.015511967147531891
1202	43	0.07200948576160471
1202	44	0.050568639852322406
1202	45	0.16262604252056434
1202	46	0.06590850672557025
1202	47	-0.20712847718744598
1202	48	0.32263920439265803
1202	49	0.1135122180707242
1202	50	-0.054082044198139556
1202	51	-0.08022014835668045
1202	52	0.24408099733653899
1202	53	0.01913859429600251
1202	54	-0.1856039542101655
1202	55	0.13980439222474672
1202	56	0.40379136789012904
1202	57	0.26796910852004885
1202	58	0.25166577465259365
1202	59	-0.02449186125202374
1202	60	0.32753310423463233
1202	61	-0.05958118130054402
1203	1	0.09118332405031282
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2403	8	0.11300602046345176
2403	9	0.029210671874849936
2403	10	-0.038023794066425864
2403	11	0.05836026586867149
2403	12	0.09027919471564161
2403	13	0.07745320810923317
2403	14	0.05917707784006154
2403	15	0.08147695021456514
2403	16	-0.06932206404184882
2403	17	-0.0413948947557401
2403	18	0.028349125172844936
2403	19	0.04175612690902142
2403	20	0.021122241910319417
2403	21	0.04306198634115077
2403	22	0.14602580798537446
2403	23	0.08663917046485636
2403	24	0.011863678559443258
2403	25	-0.018258875701107513
2403	26	0.04958760172534227
2403	27	0.0512059124498947
2403	28	0.05756473018841035
2403	29	0.03390882103600745
2403	30	0.10567373225142518
2403	31	-0.028319015466088615
2403	32	0.12443706640001295
2403	33	0.059485939656021
2403	34	-0.0887487608424351
2403	35	0.1615987658414113
2403	36	0.06571708039304269
2403	37	0.06654819680493773
2403	38	0.03760485350854026
2403	39	0.08332412762621288
2403	40	0.012741444204991126
2403	41	0.06863847011417334
2403	42	0.009816662787095254
2403	43	0.04456329155625127
2403	44	0.0034641535136543666
2403	45	0.23103876187667538
2403	46	0.18616339057646059
2403	47	0.11521344282373036
2403	48	-0.21815268961788092
2403	49	-0.22825579933547147
2403	50	0.1501212626688755
2403	51	-0.16335427283375736
2403	52	0.07268635424377834
2403	53	0.11294720891482513
2403	54	0.026533427461068015
2403	55	-0.058628325630834865
2403	56	-0.17333997283721203
2403	57	-0.3465164798834124
2403	58	-0.2739933608844538
2403	59	-0.1953742530001179
2403	60	-0.14346195693801178
2403	61	-0.13857007993428283
2404	1	-0.0569416514839034
2404	2	-0.009965534601896309
2404	3	0.030645560190777132
2404	4	0.044101140775396175
2404	5	-0.06031042615884985
2404	6	0.004369692811174275
2404	7	0.05317310073912296
2404	8	0.04311324779521186
2404	9	0.03724244077428551
2404	10	-0.019220208590333243
2404	11	0.03783743479118584
2404	12	0.029799822346411986
2404	13	-0.026464310552405834
2404	14	0.010165975705469066
2404	15	-4.976351965169101E-4
2404	16	0.013816593911847974
2404	17	-0.08838495470381957
2404	18	0.12678522190163477
2404	19	0.014985774874908486
2404	20	-0.12393180952643235
2404	21	-0.01932500576172913
2404	22	0.09454461687716531
2404	23	-0.03898780364962562
2404	24	0.007184173267056337
2404	25	-0.019444173498058603
2404	26	-0.09110465994489658
2404	27	-0.04891626542039518
2404	28	-0.1492886391217283
2404	29	8.557937177755548E-4
2404	30	-0.024001295838871783
2404	31	-0.0885363886020798
2404	32	-0.06890841839143036
2404	33	-0.0665741623047964
2404	34	-0.0612136363629574
2404	35	-0.04409451066130288
2404	36	-0.06364222203897185
2404	37	-0.09413493800593642
2404	38	-0.1169136714954746
2404	39	-0.10659347858960189
2404	40	-0.24317211064040123
2404	41	-0.09042225931822401
2404	42	-0.21659909484172601
2404	43	-0.11367097953534966
2404	44	-0.12316969360349361
2404	45	0.11068957991124506
2404	46	0.03508468335363801
2404	47	-0.43883733063780284
2404	48	-0.20530013482687792
2404	49	-0.27960963891765545
2404	50	-0.20737702272855074
2404	51	-0.016832362401672185
2404	52	-0.08440374298115216
2404	53	-0.07693126323830768
2404	54	-0.3931004634331643
2404	55	-0.24145669689767857
2404	56	-0.4315279463427899
2404	57	-0.651506802494012
2404	58	-0.3701019758172208
2404	59	-0.3484149696708343
2404	60	0.12934358391463263
2404	61	-0.3587223976657228
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	5.0
14	30.0
15	134.0
16	408.0
17	806.0
18	1098.0
19	1263.0
20	1349.0
21	1433.0
22	1605.0
23	2068.0
24	2520.0
25	2798.0
26	3528.0
27	4690.0
28	7528.0
29	9464.0
30	10479.0
31	11794.0
32	16162.0
33	18693.0
34	25231.0
35	45356.0
36	71708.0
37	100247.0
38	173353.0
39	342972.0
40	256389.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	31.822191956071755	25.41443264144434	17.80802596021159	24.955349442272308
2	28.56828423375186	21.136237862856568	20.034551779156097	30.260926124235475
3	28.20383591573527	19.593463724642017	20.332024389301694	31.87067597032102
4	28.678515728875443	18.957032176456636	20.694772853046235	31.66967924162169
5	28.26139687650479	20.919458239602122	20.686507736867448	30.132637147025637
6	27.32222813158065	20.26669373791676	22.824208165934785	29.586869964567807
7	28.68471456600953	21.859166013842273	22.440778645814614	27.01534077433358
8	26.74672449852306	23.98545698905411	23.840008911951358	25.42780960047147
9	26.375782490890405	23.933979689375374	23.7962576991354	25.893980120598826
10	29.00903053780752	22.425236633869726	22.617041232149145	25.94869159617361
11	27.46282494483933	23.720164727358974	22.755661604582468	26.061348723219226
12	28.392470838514004	22.179708780428207	22.847745779400455	26.580074601657333
13	28.965099648552883	22.361631174580815	21.4962196077304	27.177049569135903
14	29.529014151316307	22.767071058437967	21.92654467834324	25.777370111902485
15	27.926569833044653	22.2837414384177	23.267919131228485	26.521769597309163
16	28.981001013375113	22.293174451447832	21.236497315634008	27.489327219543046
17	29.859708636687053	22.909374797864007	21.380328304788737	25.850588260660203
18	27.882459267351358	22.413288150698225	22.992205636090528	26.712046945859896
19	30.26757415246624	22.317071417790842	21.18268422225257	26.23267020749035
20	28.600266639834988	22.699692393937	22.80372505192649	25.896315914301525
21	28.36264454969491	23.66608211931953	21.783612071381857	26.187661259603708
22	30.14476530663581	22.71855841999727	21.115036043093596	26.021640230273324
23	29.90157324689699	23.06847828430562	21.464237201647272	25.565711267150114
24	29.569261673578218	22.617759937903823	21.789451555638607	26.02352683287935
25	29.304059250102416	24.35397336476473	20.740410668468222	25.601556716664632
26	28.835283421614356	24.819155664479407	21.22805252301655	25.117508390889686
27	28.697830946032383	24.456927964122208	21.36523548394052	25.480005605904886
28	29.276748431424693	25.0417747719906	20.578342520788564	25.103134275796148
29	29.349427550866398	25.4507183464018	20.758108797677146	24.441745305054656
30	29.012893581238906	25.494200044559754	20.718310466511905	24.774595907689434
31	30.038037502066278	24.638580843616815	21.343764149519544	23.97961750479736
32	30.735810951638285	25.447484170505753	20.196440250397085	23.620264627458873
33	29.736654625231672	25.494451985938383	21.139141910752034	23.62975147807791
34	30.480939923385968	25.751496704734116	20.55354717225221	23.21401619962771
35	30.556493865846384	26.168795233543435	20.598556120138852	22.67615478047133
36	30.543916515139536	26.112826022897966	19.42158560863597	23.92167185332653
37	32.334392226478556	26.335175615751155	18.850304371887106	22.48012778588318
38	31.602570091778727	26.64835164835165	19.648876303552562	22.100201956317065
39	31.803538188430274	26.72489381122475	18.53802672147996	22.933541278865018
40	32.617113102724616	27.228347192375967	18.056763380504385	22.09777632439503
41	32.77909141218493	27.119103917665065	18.02064841633187	22.081156253818122
42	32.616304558750606	27.825771350951207	18.36940038378887	21.18852370650932
43	33.664447063727636	26.745556601671712	18.606573282832276	20.983423051768376
44	33.57811253494707	26.919393556084202	19.241729493528055	20.260764415440676
45	34.39573016911147	26.532100992532648	18.924690417496173	20.147478420859716
46	35.25443554146891	25.547586898341674	17.357029083352874	21.84094847683654
47	35.62786134728581	25.655279971826733	17.273823299003155	21.443035381884304
48	35.40335563716859	27.37065093180201	17.7347832024091	19.4912102286203
49	36.84678630721796	25.747364146644724	16.80684423490179	20.599005311235526
50	36.03024673168558	27.45887206318861	17.650245438015222	18.860635767110587
51	35.904113871739774	27.676280553978394	17.844295991778004	18.575309582503827
52	36.19518970238394	27.99915911426703	17.557262881003886	18.248388302345138
53	37.19581819929584	25.60441070910396	16.526503693708346	20.673267397891852
54	35.690658262600714	25.445777244338398	16.800555559548368	22.06300893351253
55	37.15735703145775	25.570472692774853	18.069879760527243	19.202290515240154
56	34.86405680650285	27.2933002249549	20.817491860657327	17.02515110788492
57	35.5206843516196	28.731071087186194	19.745721903995285	16.002522657198917
58	33.7432351820841	29.703030782167474	20.79125910061162	15.762474935136805
59	33.0742998009185	30.457042957042958	19.902489596734203	16.566167645304336
60	32.22643265523202	26.966026656892232	24.71826633174858	16.089274356127166
61	32.383443894235256	29.57779630441501	21.36676273366921	16.671997067680522
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	3.5
2	1.0
3	1.0
4	3.0
5	5.0
6	4.0
7	2.5
8	2.0
9	4.5
10	7.0
11	5.0
12	4.5
13	6.0
14	6.5
15	7.0
16	10.0
17	16.5
18	20.0
19	29.5
20	45.5
21	52.0
22	79.0
23	106.0
24	175.5
25	325.0
26	405.0
27	550.5
28	696.0
29	1009.5
30	1703.5
31	2084.0
32	2614.5
33	3145.0
34	4058.0
35	6041.5
36	7112.0
37	8847.5
38	10583.0
39	12616.0
40	17933.5
41	21218.0
42	24693.0
43	32877.5
44	37587.0
45	42127.0
46	46667.0
47	51820.0
48	62730.5
49	68488.0
50	72056.5
51	75625.0
52	78143.0
53	83250.5
54	85840.0
55	87917.0
56	89994.0
57	89548.0
58	86295.5
59	83489.0
60	79814.5
61	70466.5
62	64793.0
63	58116.5
64	51440.0
65	44988.5
66	32694.5
67	26852.0
68	22423.0
69	17994.0
70	14568.5
71	8972.0
72	6801.0
73	5434.5
74	4068.0
75	3165.5
76	1792.5
77	1322.0
78	1027.0
79	732.0
80	549.5
81	288.0
82	209.0
83	163.5
84	95.0
85	72.0
86	73.5
87	75.0
88	56.5
89	36.5
90	35.0
91	37.0
92	39.0
93	44.0
94	50.5
95	52.0
96	61.0
97	70.0
98	88.0
99	303.5
100	501.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0021561172640309333
2	0.0
3	8.983821933462222E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	9.882204126808443E-4
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	8.983821933462222E-5
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	2.6951465800386667E-4
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	3.5935287733848887E-4
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1113112.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.53380133749833
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.04857856996185	74.38566271649803
2	8.25880731261963	13.797791386740933
3	1.5127778790570487	3.7910426045074073
4	0.4060957959665895	1.3569090217706536
5	0.19459112493697278	0.8127468186262701
6	0.1086692255643951	0.5446532099877585
7	0.08504773890807389	0.4973052648305329
8	0.06622229184616707	0.44254398169532433
9	0.05016075805673873	0.37711069186049384
>10	0.265765514402192	3.3927444942962057
>50	0.0019249795712248427	0.1117093040806936
>100	0.0010191068318249165	0.209608651856746
>500	2.2646818484998145E-4	0.10707445573187564
>1k	1.1323409242499072E-4	0.17309739751707812
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTTTCGTCACATTTCCACCCCCCTTGTCTC	1830	0.16440394138235864	Illumina Single End PCR Primer 1 (96% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.159991088048642	0.0	0.0	0.0	0.0
2	1.1625065581900114	0.0	0.0	0.0	0.0
3	1.163584616822027	0.0	0.0	0.0	0.0
4	1.1645728372347077	0.0	0.0	0.0	0.0
5	1.1667289544987387	0.0	0.0	0.0	0.0
6	1.1713107036848045	0.0	0.0	0.0	0.0
7	1.176880673283551	0.0	0.0	0.0	0.0
8	1.1935905820797907	0.0	0.0	0.0	8.983821933462222E-5
9	1.199699580994545	0.0	0.0	0.0	8.983821933462222E-5
10	1.2102106526566958	0.0	0.0	0.0	8.983821933462222E-5
11	1.2328498839290207	0.0	0.0	0.0	8.983821933462222E-5
12	1.2739957883842776	0.0	0.0	0.0	8.983821933462222E-5
13	1.3340077188998052	0.0	0.0	0.0	8.983821933462222E-5
14	1.413963734107619	0.0	0.0	0.0	8.983821933462222E-5
15	1.5130552900337073	0.0	0.0	0.0	1.7967643866924444E-4
16	1.6333486657227665	0.0	0.0	0.0	1.7967643866924444E-4
17	1.752833497437814	0.0	0.0	0.0	1.7967643866924444E-4
18	1.915710189091484	0.0	0.0	0.0	2.695146580038666E-4
19	2.1220685789031113	0.0	0.0	0.0	2.695146580038666E-4
20	2.382329900315512	0.0	0.0	0.0	3.5935287733848887E-4
21	2.7429405127246853	0.0	8.983821933462222E-5	0.0	3.5935287733848887E-4
22	3.199408505163901	0.0	8.983821933462222E-5	0.0	3.5935287733848887E-4
23	3.7671860513587134	0.0	8.983821933462222E-5	0.0	3.5935287733848887E-4
24	4.4705294705294705	0.0	8.983821933462222E-5	0.0	3.5935287733848887E-4
25	5.340702463004622	0.0	1.7967643866924444E-4	0.0	4.491910966731111E-4
26	6.358479649846556	0.0	1.7967643866924444E-4	0.0	4.491910966731111E-4
27	7.53230582367273	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
28	8.900272389481023	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
29	10.408566253889996	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
30	12.097884130258231	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
31	13.969753268314419	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
32	16.017076448731125	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
33	18.18038077030883	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
34	20.52327169233644	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
35	22.838402604589653	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
36	25.24840267646023	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
37	27.697482373741366	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
38	30.157971524878	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
39	32.73156699415692	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
40	35.419257001990815	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
41	38.07792926497962	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
42	40.765170081716846	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
43	43.47451110041038	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
44	46.12500808543974	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
45	48.71782893365627	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
46	51.22557298816292	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
47	53.5940678027009	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
48	55.9273460352597	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
49	58.18354307562941	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
50	60.348374646935795	0.0	2.695146580038666E-4	0.0	4.491910966731111E-4
>>END_MODULE