##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-32B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 639682 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.55666096591744 40.0 40.0 40.0 40.0 40.0 2 39.33146782307459 40.0 40.0 40.0 40.0 40.0 3 39.27571512095072 40.0 40.0 40.0 40.0 40.0 4 39.13666478031272 40.0 40.0 40.0 40.0 40.0 5 39.18982869613339 40.0 40.0 40.0 40.0 40.0 6 39.33066742537699 40.0 40.0 40.0 40.0 40.0 7 39.343279942221294 40.0 40.0 40.0 40.0 40.0 8 39.35353503772187 40.0 40.0 40.0 40.0 40.0 9 39.37588989529172 40.0 40.0 40.0 40.0 40.0 10-11 39.369042743112985 40.0 40.0 40.0 40.0 40.0 12-13 39.28870907732279 40.0 40.0 40.0 40.0 40.0 14-15 39.32672796795908 40.0 40.0 40.0 40.0 40.0 16-17 39.42939773199809 40.0 40.0 40.0 40.0 40.0 18-19 39.4637929471206 40.0 40.0 40.0 40.0 40.0 20-21 39.43656066608096 40.0 40.0 40.0 40.0 40.0 22-23 39.48193946367101 40.0 40.0 40.0 40.0 40.0 24-25 39.45578271703753 40.0 40.0 40.0 40.0 40.0 26-27 39.42145628609215 40.0 40.0 40.0 40.0 40.0 28-29 39.4349848831138 40.0 40.0 40.0 40.0 40.0 30-31 39.404885460170334 40.0 40.0 40.0 40.0 40.0 32-33 39.40466171738142 40.0 40.0 40.0 40.0 40.0 34-35 39.40067348408192 40.0 40.0 40.0 40.0 40.0 36-37 39.38012650449291 40.0 40.0 40.0 40.0 40.0 38-39 39.31649701381507 40.0 40.0 40.0 40.0 40.0 40-41 39.35466404100683 40.0 40.0 40.0 40.0 40.0 42-43 39.33837011759169 40.0 40.0 40.0 40.0 40.0 44-45 39.22591083873976 40.0 40.0 40.0 40.0 40.0 46-47 39.21175821325666 40.0 40.0 40.0 40.0 40.0 48-49 39.311840974584 40.0 40.0 40.0 40.0 40.0 50-51 39.25016660866469 40.0 40.0 40.0 40.0 40.0 52-53 39.221102460770425 40.0 40.0 40.0 40.0 40.0 54-55 39.12890740952035 40.0 40.0 40.0 40.0 40.0 56-57 39.1341960742566 40.0 40.0 40.0 40.0 40.0 58-59 39.089424679410016 40.0 40.0 40.0 40.0 40.0 60-61 38.994719847327815 40.0 40.0 40.0 40.0 40.0 62-63 39.36229197523069 40.0 40.0 40.0 40.0 40.0 64-65 39.37241477281169 40.0 40.0 40.0 40.0 40.0 66-67 39.36924735298414 40.0 40.0 40.0 40.0 40.0 68-69 39.38795431751065 40.0 40.0 40.0 40.0 40.0 70-71 39.38152100399974 40.0 40.0 40.0 40.0 40.0 72-73 39.39399054658951 40.0 40.0 40.0 40.0 40.0 74-75 39.38025778946714 40.0 40.0 40.0 40.0 40.0 76-77 39.37097688022445 40.0 40.0 40.0 40.0 40.0 78-79 39.38503092270152 40.0 40.0 40.0 40.0 40.0 80-81 39.365950651425585 40.0 40.0 40.0 40.0 40.0 82-83 39.395054563450486 40.0 40.0 40.0 40.0 40.0 84-85 39.333224958412686 40.0 40.0 40.0 40.0 40.0 86-87 39.343888354784596 40.0 40.0 40.0 40.0 40.0 88-89 39.394379610155 40.0 40.0 40.0 40.0 40.0 90-91 39.54238751980458 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04570355926487224 1101 2 -0.09343742916065167 1101 3 -0.09373758895223716 1101 4 -0.17678988506585824 1101 5 -0.02560548780328986 1101 6 -0.04534587112938482 1101 7 0.026519914924726606 1101 8 -0.05509832503224743 1101 9 0.011319860852928798 1101 10-11 -0.05992885073285237 1101 12-13 -0.23603508805349094 1101 14-15 0.02976168263075607 1101 16-17 -0.018001988094638932 1101 18-19 0.014998309473355675 1101 20-21 0.11355149627220129 1101 22-23 0.06422440735452284 1101 24-25 0.013518061477711285 1101 26-27 -0.04022244930703067 1101 28-29 0.08762893136425731 1101 30-31 0.04885277437195157 1101 32-33 0.08640429264247018 1101 34-35 -0.05298970064288966 1101 36-37 -0.04688408340126671 1101 38-39 -0.04581067617929335 1101 40-41 -0.13042543185505906 1101 42-43 0.0012324845646602967 1101 44-45 0.07807307896490556 1101 46-47 -0.12168287469243211 1101 48-49 0.023999281448773502 1101 50-51 -0.12796417102527613 1101 52-53 0.17534419379187938 1101 54-55 -0.11719287721474814 1101 56-57 -0.1299158098282831 1101 58-59 -0.08274442157631512 1101 60-61 -0.045978006045992004 1101 62-63 -0.20886434897679607 1101 64-65 -0.5220104038495705 1101 66-67 -0.33206484539677916 1101 68-69 -0.2549458839607297 1101 70-71 0.2382364037866438 1101 72-73 -0.3162596227218515 1101 74-75 -0.2523706651653086 1101 76-77 0.07569414513236694 1101 78-79 0.4824901124037595 1101 80-81 0.06052271273794929 1101 82-83 0.09137127686423696 1101 84-85 0.593163387912675 1101 86-87 -0.2845492567815384 1101 88-89 0.399830439651943 1101 90-91 0.26976686507936165 1102 1 -0.0627268516980024 1102 2 -0.09703771472815248 1102 3 -0.06051568032049204 1102 4 -0.15109681674984188 1102 5 -0.1444002328275431 1102 6 0.010250384466871765 1102 7 -0.18896211367205495 1102 8 -0.013365437397723667 1102 9 -0.017957479899820328 1102 10-11 -0.04228438226871134 1102 12-13 -0.01632692736171748 1102 14-15 -0.04973150178045671 1102 16-17 -0.03413721898397171 1102 18-19 -0.046386025681478316 1102 20-21 -0.02533572458223432 1102 22-23 -2.4276566293224278E-5 1102 24-25 -0.11842746718912878 1102 26-27 -0.04189849032733406 1102 28-29 -0.1253743980325126 1102 30-31 -0.1053203142273702 1102 32-33 -0.13898266886265986 1102 34-35 -0.07587706932481808 1102 36-37 -0.1402386550406618 1102 38-39 -0.10487766128881759 1102 40-41 -0.13390231844388722 1102 42-43 -0.036606450048900285 1102 44-45 -0.08416705880294018 1102 46-47 0.024767899386716863 1102 48-49 -0.07908310294129706 1102 50-51 -0.012131384283399882 1102 52-53 -0.09215234170351039 1102 54-55 -0.1768911651221572 1102 56-57 0.06323923803722664 1102 58-59 -0.043499790748214195 1102 60-61 0.03573466255630109 1102 62-63 -0.06613720218637553 1102 64-65 -0.35992959587397877 1102 66-67 -0.398772934306983 1102 68-69 -0.4512699587467921 1102 70-71 -0.17450573912791612 1102 72-73 -0.03907102698313025 1102 74-75 -0.17093232208762288 1102 76-77 -0.09360606537328664 1102 78-79 -0.0838748951377184 1102 80-81 -0.0469447407174286 1102 82-83 0.03609583335349953 1102 84-85 -0.46275766471890023 1102 86-87 0.6070916100915227 1102 88-89 -0.17159813177662642 1102 90-91 0.26976686507936165 1103 1 -9.86532558577835E-4 1103 2 0.21623953285402564 1103 3 0.09793231579566708 1103 4 0.1345227455513367 1103 5 0.12007666214262258 1103 6 0.09943311669893262 1103 7 -0.027160470704494344 1103 8 -0.22900429115316712 1103 9 0.0731339564830975 1103 10-11 -0.00538468505511247 1103 12-13 -0.04518170487120443 1103 14-15 -0.014351485682958298 1103 16-17 0.11695511170477602 1103 18-19 0.06502403909181709 1103 20-21 0.09827650441116731 1103 22-23 -0.023022628488412522 1103 24-25 0.06145129049231457 1103 26-27 0.029838251588778064 1103 28-29 -0.0357655929528633 1103 30-31 0.08086777106385057 1103 32-33 0.07506716213796238 1103 34-35 0.03898091502773582 1103 36-37 -0.026105309968315282 1103 38-39 0.04478314236306602 1103 40-41 0.07639517435820409 1103 42-43 0.002242289007938325 1103 44-45 -0.062368418658785174 1103 46-47 0.07725934290360215 1103 48-49 -1.216920439048863E-4 1103 50-51 0.061840191279088685 1103 52-53 0.09648101198112613 1103 54-55 0.10562286985617675 1103 56-57 0.11785974835371604 1103 58-59 0.019274195519336956 1103 60-61 -0.028082729020283637 1103 62-63 -0.021425346606342544 1103 64-65 -0.10424416894405653 1103 66-67 -0.005302313368545697 1103 68-69 0.28762256258081464 1103 70-71 0.17438598096633484 1103 72-73 -0.29502254669324657 1103 74-75 -0.28729249130664414 1103 76-77 -0.12170883886528117 1103 78-79 -0.41390457450325613 1103 80-81 -0.3250530830714027 1103 82-83 -0.5104533237859101 1103 84-85 -0.34059959096308745 1103 86-87 0.4119696588720103 1103 88-89 0.399830439651943 1103 90-91 0.26976686507936165 1104 1 -0.07497756394871402 1104 2 0.07631520549976756 1104 3 0.14089355858874342 1104 4 -0.1680076836607043 1104 5 0.0852332124309001 1104 6 -7.030139865236151E-4 1104 7 -0.07542680638675137 1104 8 0.07403662812934186 1104 9 -0.09406655600889735 1104 10-11 0.0845566586348312 1104 12-13 -0.05528640538370411 1104 14-15 0.055705769281807704 1104 16-17 -0.06293758528433102 1104 18-19 -0.03557253361798729 1104 20-21 -0.19772265478166418 1104 22-23 -0.09539210318412472 1104 24-25 0.06467438466272313 1104 26-27 -0.04583939895574218 1104 28-29 -0.0632419702750937 1104 30-31 0.042791882644252155 1104 32-33 -0.1531565605377665 1104 34-35 -0.04540082302616355 1104 36-37 -0.17641150417033202 1104 38-39 -0.16270269234954782 1104 40-41 -0.06909065698482664 1104 42-43 -0.14112930565474358 1104 44-45 -0.054503630287506155 1104 46-47 -0.02582996102420765 1104 48-49 -0.10018985784930123 1104 50-51 0.008879798754087176 1104 52-53 0.12316689835623151 1104 54-55 0.026308449287256508 1104 56-57 -0.11046583688256817 1104 58-59 -0.04383957008141692 1104 60-61 0.16450145833037055 1104 62-63 0.19931593704863104 1104 64-65 0.14231332698392407 1104 66-67 0.17829707503264558 1104 68-69 0.11723136071850604 1104 70-71 0.29944530982312756 1104 72-73 0.3181493734347498 1104 74-75 -0.10964397924418989 1104 76-77 0.3340014993917535 1104 78-79 0.5282417463906839 1104 80-81 0.6013152535304869 1104 82-83 0.17947913930932202 1104 84-85 0.3645919593412472 1104 86-87 0.2737582767581941 1104 88-89 0.399830439651943 1104 90-91 0.26976686507936165 1201 1 0.034230745989525246 1201 2 -0.034867065696190025 1201 3 -0.008676305853377642 1201 4 0.029974990599342277 1201 5 -0.1371300766522765 1201 6 -0.06772495190262617 1201 7 0.07499280137775344 1201 8 -0.20353741735072362 1201 9 -0.017624749640084758 1201 10-11 -0.027810615970132346 1201 12-13 -0.07898835352679612 1201 14-15 0.05073640014729364 1201 16-17 -0.003966902682265072 1201 18-19 0.00584430684082804 1201 20-21 0.06832784353388632 1201 22-23 0.03670914410873394 1201 24-25 -0.012336423871808222 1201 26-27 0.06597265987958423 1201 28-29 0.00365839669370871 1201 30-31 0.09236305133920553 1201 32-33 0.0017541999900956284 1201 34-35 7.819663828385615E-4 1201 36-37 0.08288066061501809 1201 38-39 0.11362388984580463 1201 40-41 0.06954826942752845 1201 42-43 0.14021420139382457 1201 44-45 0.0246696587275963 1201 46-47 0.04295166322565791 1201 48-49 0.03349027983419717 1201 50-51 -0.12172738355219792 1201 52-53 0.1126918951872895 1201 54-55 0.07641856544388759 1201 56-57 -0.01181369908295693 1201 58-59 0.043638944920843414 1201 60-61 0.11983960611811284 1201 62-63 0.1306704136085841 1201 64-65 0.23498905468680675 1201 66-67 0.17924993821589652 1201 68-69 0.18718030636541982 1201 70-71 0.07918949725963387 1201 72-73 0.07584115982673012 1201 74-75 -0.02165027444491585 1201 76-77 -0.2777808613820909 1201 78-79 -0.24706520613145955 1201 80-81 0.17054602276125763 1201 82-83 0.34851413400708964 1201 84-85 0.593163387912675 1201 86-87 0.6070916100915227 1201 88-89 0.399830439651943 1201 90-91 -1.063566468253967 1202 1 0.16072482361374796 1202 2 0.08571102726545377 1202 3 0.11492088467742434 1202 4 0.08147672976041065 1202 5 0.013367136044763583 1202 6 -0.04621840226288043 1202 7 0.06595546140098918 1202 8 0.03472686966182437 1202 9 -0.09049711046277054 1202 10-11 0.0173785780050153 1202 12-13 -4.724875206036927E-4 1202 14-15 0.042160467068754315 1202 16-17 0.08903651640520138 1202 18-19 -0.03162879204584357 1202 20-21 0.04030049208118669 1202 22-23 -0.05791044616817942 1202 24-25 -0.05069554870762971 1202 26-27 0.03945485721146724 1202 28-29 -0.02570454067248562 1202 30-31 0.016821389258034003 1202 32-33 0.020318814553135667 1202 34-35 -0.009378369604227998 1202 36-37 -0.2040170782051618 1202 38-39 -0.16597478951470634 1202 40-41 -0.0680887272835804 1202 42-43 -0.17778067827141086 1202 44-45 -0.1961787204729717 1202 46-47 0.022784159642981194 1202 48-49 -0.23306108432917227 1202 50-51 0.010724653833698028 1202 52-53 -0.07241573210495744 1202 54-55 -0.1502625499315826 1202 56-57 0.1605762359051326 1202 58-59 -0.3233848984017058 1202 60-61 0.13353848078754993 1202 62-63 -0.12716676902618218 1202 64-65 -0.24333080570627885 1202 66-67 -0.16470203027288477 1202 68-69 -0.13328522603534054 1202 70-71 -0.07135056097273207 1202 72-73 -0.11282904336167832 1202 74-75 0.2620158801889758 1202 76-77 -0.3201146886245638 1202 78-79 0.3476108442407977 1202 80-81 -0.7949253479732761 1202 82-83 0.02210720760017182 1202 84-85 -0.3039942348263409 1202 86-87 -0.7147757117757934 1202 88-89 -0.34122219192700953 1202 90-91 -2.930233134920641 1203 1 -0.049101103180767325 1203 2 0.0021551470690468477 1203 3 -0.03334028352907126 1203 4 -0.011536581029332638 1203 5 -0.15313654072912897 1203 6 0.010063938871411438 1203 7 -0.11121810537712662 1203 8 -0.017843936700927543 1203 9 -0.1032087771711474 1203 10-11 -0.016286636738414018 1203 12-13 -8.995055903895377E-4 1203 14-15 -0.07389142644121449 1203 16-17 -0.08343305392254052 1203 18-19 -0.020639410953222637 1203 20-21 -0.02537377470375901 1203 22-23 -0.014669768340333178 1203 24-25 -0.01526788022319181 1203 26-27 0.04048192527512384 1203 28-29 0.0397707393997635 1203 30-31 -0.023588245365672833 1203 32-33 -0.051658227426216285 1203 34-35 0.015681662237852834 1203 36-37 0.052320914514382366 1203 38-39 -0.02318787335914152 1203 40-41 0.0071142810220550246 1203 42-43 0.12359262682201688 1203 44-45 0.019782916344816215 1203 46-47 -0.027853835077849 1203 48-49 -0.07546773102234994 1203 50-51 -0.12069801732174312 1203 52-53 0.006225648788429794 1203 54-55 -0.15290652609965605 1203 56-57 0.12114770266099839 1203 58-59 -0.04465334730172543 1203 60-61 -0.23500986841735738 1203 62-63 -0.12826627347497066 1203 64-65 -0.4200898513673934 1203 66-67 0.020846811741371596 1203 68-69 0.19761358790467654 1203 70-71 -0.11564353215250378 1203 72-73 0.10253312693615158 1203 74-75 0.10609240498867933 1203 76-77 0.10691203660434923 1203 78-79 -0.012607926811931236 1203 80-81 0.4609643763375004 1203 82-83 0.5485141340070925 1203 84-85 0.3769471716964574 1203 86-87 0.33122954112600667 1203 88-89 0.399830439651943 1203 90-91 0.26976686507936165 1204 1 -0.07253324021161234 1204 2 0.014825186488359066 1204 3 -0.21519688138952375 1204 4 -0.3555416326015006 1204 5 -0.19734928349522818 1204 6 -0.2783714007328584 1204 7 -0.10362140171537249 1204 8 -0.16261295090379235 1204 9 -0.17500083085952411 1204 10-11 -0.25912119986599436 1204 12-13 -0.09299094775197858 1204 14-15 -0.17649649850743288 1204 16-17 -0.1557879383533134 1204 18-19 -0.027000426067736782 1204 20-21 -0.09757752305977618 1204 22-23 -0.12365459639433851 1204 24-25 -0.03474508844971069 1204 26-27 -0.1715480830947982 1204 28-29 -0.14769844773299212 1204 30-31 -0.12390615030369645 1204 32-33 -0.13711474185734573 1204 34-35 -0.03368751472912379 1204 36-37 -0.07159344686873226 1204 38-39 -0.08420924805867003 1204 40-41 -0.07124650194926119 1204 42-43 -0.2092180219851585 1204 44-45 -0.13364825728799445 1204 46-47 -0.018632687763734168 1204 48-49 0.013400927237938731 1204 50-51 -0.0909756182458139 1204 52-53 -0.18932175899945491 1204 54-55 -0.4259375794654545 1204 56-57 -0.06449317819584621 1204 58-59 0.07413215280796948 1204 60-61 -0.12392137962806515 1204 62-63 -0.20821757864074897 1204 64-65 0.015735971253810987 1204 66-67 -0.02607290913913829 1204 68-69 0.004469945848555312 1204 70-71 -0.3277583093352945 1204 72-73 0.2598914548827125 1204 74-75 -0.09036622282741291 1204 76-77 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0.10418550673533389 2401 52-53 -0.07707547514418422 2401 54-55 0.018785618054046438 2401 56-57 -0.1451724497568776 2401 58-59 0.1677220188748052 2401 60-61 0.16250870852352506 2401 62-63 0.25535284922008117 2401 64-65 0.16276960613110703 2401 66-67 0.25226333131143264 2401 68-69 0.19216566033739468 2401 70-71 0.09841101976108746 2401 72-73 -0.12649386330360102 2401 74-75 0.4807730867570683 2401 76-77 -0.035145234232324185 2401 78-79 0.15940298281508092 2401 80-81 0.467981920197154 2401 82-83 0.39757073778067564 2401 84-85 -0.102211412725282 2401 86-87 -0.6252921980044235 2401 88-89 -0.5779473381258313 2401 90-91 0.26976686507936165 2402 1 -0.04627665760430233 2402 2 0.02413970916830266 2402 3 0.07040909592150513 2402 4 0.12364099998193723 2402 5 0.1953769867079842 2402 6 -0.026787390335250905 2402 7 0.04659250738105669 2402 8 0.10835498160366086 2402 9 0.12475088033119874 2402 10-11 0.033793953628354245 2402 12-13 0.1095785870634316 2402 14-15 0.16401527510831215 2402 16-17 0.07983863396469815 2402 18-19 0.06440858420679518 2402 20-21 0.06712345549023979 2402 22-23 0.12432898011324767 2402 24-25 0.11823232596613309 2402 26-27 0.13264099055655976 2402 28-29 0.07182627530108476 2402 30-31 0.05638795624223292 2402 32-33 0.12385745056867847 2402 34-35 0.11599243152938499 2402 36-37 0.0772431030646814 2402 38-39 -0.25979873334063797 2402 40-41 0.021408741641948836 2402 42-43 0.08854969127055057 2402 44-45 0.1479291361643078 2402 46-47 0.09318035000505631 2402 48-49 0.026950628283756828 2402 50-51 -0.005102785559891743 2402 52-53 0.10202924411748882 2402 54-55 0.1022193717889408 2402 56-57 -0.031425732484891 2402 58-59 -0.03629202138522203 2402 60-61 0.18220495352556298 2402 62-63 0.014590462689923811 2402 64-65 -0.10390384704463429 2402 66-67 -0.3612289867448837 2402 68-69 -0.2639301076310261 2402 70-71 -0.19124875063836555 2402 72-73 0.009841504832763803 2402 74-75 0.38039581530880895 2402 76-77 0.24763745906351886 2402 78-79 0.5533313521254541 2402 80-81 0.19566698652150194 2402 82-83 0.20305958855254858 2402 84-85 0.29196704730747314 2402 86-87 -0.02448733727690211 2402 88-89 0.0798304396519427 2402 90-91 -0.17467757936508121 2403 1 0.015506718124349561 2403 2 0.062442541948364294 2403 3 0.027962681055065275 2403 4 0.15232852218952075 2403 5 0.23570467438016607 2403 6 0.06910976669073676 2403 7 0.13441737980089385 2403 8 0.048933080975558596 2403 9 0.07816308351046075 2403 10-11 0.07053322587599808 2403 12-13 0.09918186291342579 2403 14-15 0.09393666805593881 2403 16-17 0.08161120704482983 2403 18-19 0.115079092792989 2403 20-21 0.05253621310279044 2403 22-23 0.06761690883190141 2403 24-25 0.034698811170791544 2403 26-27 0.017296866064285155 2403 28-29 0.09648425560183682 2403 30-31 0.07921616002873577 2403 32-33 0.10973342983132284 2403 34-35 0.004411120271669233 2403 36-37 0.05213836127860816 2403 38-39 0.016201520082560705 2403 40-41 0.11779928642695126 2403 42-43 0.08757529101176686 2403 44-45 -0.06415878475383607 2403 46-47 -0.007433153565379769 2403 48-49 -0.010753567788341911 2403 50-51 -0.06276003110441053 2403 52-53 0.010124102705120208 2403 54-55 -0.10710175176193104 2403 56-57 0.0057132046675079096 2403 58-59 0.026089270404447973 2403 60-61 0.07730258938827461 2403 62-63 0.17689078225235022 2403 64-65 0.21900337676643744 2403 66-67 0.2502297978471688 2403 68-69 0.19219663617015215 2403 70-71 0.07027458965404776 2403 72-73 0.11610212260954 2403 74-75 0.1619989005557514 2403 76-77 0.07065897940085364 2403 78-79 -0.17410582631809035 2403 80-81 -0.07924030202507026 2403 82-83 -0.013390627897663876 2403 84-85 -0.395632130294608 2403 86-87 0.3717974924444647 2403 88-89 0.399830439651943 2403 90-91 0.26976686507936165 2404 1 0.10883673968993435 2404 2 -0.039294110685929695 2404 3 0.11062753122422464 2404 4 -0.017278759719737025 2404 5 0.18109959380151963 2404 6 0.04158727101078341 2404 7 0.015425422208579675 2404 8 0.15697133118245432 2404 9 -0.07906984214141488 2404 10-11 0.022488430308364116 2404 12-13 0.14264099722728218 2404 14-15 0.07286927678017463 2404 16-17 0.10005832198698528 2404 18-19 -0.017332631007000998 2404 20-21 0.0015957527883898592 2404 22-23 0.06284824753583962 2404 24-25 0.1126799262771101 2404 26-27 0.11045774584321322 2404 28-29 0.045933247804612165 2404 30-31 0.047803758595577506 2404 32-33 0.023468777462468893 2404 34-35 0.06002598429470396 2404 36-37 0.10979345575501043 2404 38-39 0.12736033550024928 2404 40-41 0.05103132213589845 2404 42-43 0.16012479802855495 2404 44-45 0.11567015072436249 2404 46-47 0.07055450935533258 2404 48-49 0.07442870562393011 2404 50-51 0.2437601193069412 2404 52-53 -3.084057103848181E-4 2404 54-55 0.0031112993199400307 2404 56-57 -0.06758557567660972 2404 58-59 -0.021538175373137847 2404 60-61 -0.19321705636846076 2404 62-63 -0.09229811994818249 2404 64-65 0.10871750621772946 2404 66-67 0.0031554797554207425 2404 68-69 -0.43121374312088534 2404 70-71 -0.39994974395234806 2404 72-73 -0.31755073482941754 2404 74-75 -0.28860074139538483 2404 76-77 0.04816542583188976 2404 78-79 0.15153170301397978 2404 80-81 0.16016647763594705 2404 82-83 -0.3431267328659686 2404 84-85 0.028738497906388716 2404 86-87 0.3570916100915227 2404 88-89 0.399830439651943 2404 90-91 0.26976686507936165 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 19.0 31 463.0 32 2802.0 33 5317.0 34 6980.0 35 8645.0 36 12545.0 37 18824.0 38 47424.0 39 242157.0 40 294506.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.138328106778054 13.537664026813323 28.188068446509355 31.135939419899263 2 27.896283789065553 16.79314603755296 27.277175867298283 28.033394306083203 3 28.00453975569111 18.9208075262396 21.096107128229338 31.978545589839953 4 27.990782920263506 20.450786484534504 19.594892462192153 31.963538133009838 5 27.723618923152443 20.26366225718404 20.311029542804082 31.70168927685944 6 28.561222607483096 22.585909873968628 22.795701614239576 26.057165904308704 7 26.834739761318904 22.474291913794666 22.411135532967943 28.279832791918487 8 25.727627164747485 23.325339778202295 23.011433806172445 27.93559925087778 9 25.94117076922596 23.203247863782316 22.946401493241954 27.909179873749768 10-11 25.563092286479844 22.317729746968023 23.029411488833514 29.089766477718616 12-13 26.452362267501666 22.072529788238533 22.462489174308484 29.01261876995132 14-15 26.42117489627659 21.710162236861443 22.55151778539962 29.317145081462353 16-17 26.495586869725894 21.549222895126015 22.269815314484383 29.685374920663705 18-19 26.822780694157412 21.714930230958508 22.151397100434277 29.310891974449802 20-21 26.71139722549642 21.778008447947574 22.1281824406502 29.382411885905807 22-23 26.510437998880693 21.610737835361945 22.286307884229977 29.59251628152738 24-25 26.78369877532899 21.886734346128232 22.19180780450286 29.137759074039916 26-27 26.762360047648677 21.99123939707542 22.206737097495317 29.039663457780584 28-29 26.88765667941258 21.80098861621868 22.20916017646268 29.102194527906057 30-31 27.22951556484865 21.86535868524417 22.02184745862076 28.88327829128642 32-33 27.099172625144313 21.83774594495132 22.16244294598208 28.900638483922286 34-35 26.934067628898852 21.58714589965359 22.435409774703192 29.043376696744367 36-37 27.092652350551365 21.779222286709228 22.342426001160767 28.785699361578644 38-39 27.360992249557032 21.633639895696476 22.38749000558482 28.617877849161676 40-41 27.091140873625363 21.598728266879647 22.466078340164476 28.84405251933052 42-43 27.233777013068057 21.735864145547957 22.456212627000042 28.57414621438395 44-45 27.16977920205788 21.755548320068524 22.553058111618945 28.52161436625465 46-47 27.141039429237924 21.530211915112773 22.748837420664376 28.579911234984927 48-49 27.452522601988328 21.697908793576755 22.537667136990486 28.311901467444432 50-51 27.270861568247824 21.62245885769603 22.580058083252663 28.526621490803482 52-53 27.272586325278525 21.49593567963853 22.801612438813706 28.42986555626924 54-55 27.464503559194693 21.73515516560761 22.62603624138853 28.174305033809166 56-57 27.50358283995865 21.80862935814303 22.684663095573722 28.003124706324595 58-59 27.45649427395362 21.7474290527231 22.710600317887273 28.085476355436008 60-61 27.866206853012198 21.710806673085116 22.42893669407661 27.99404977982607 62-63 27.826499935446986 21.841513122706065 22.399435622198858 27.93255131964809 64-65 27.89529578777403 21.620303644382798 22.773328709673923 27.711071858169245 66-67 28.13511701414387 21.823058012196288 22.366463395166182 27.675361578493664 68-69 28.017889384976847 21.633464028586754 22.46371884655773 27.884927739878673 70-71 27.667025862068968 21.832794540229887 22.625718390804597 27.87446120689655 72-73 28.477878761896598 21.65287661836577 22.054788647860757 27.81445597187688 74-75 28.668676959712176 21.37079553249039 22.001061227659218 27.95946628013822 76-77 28.270433334380797 21.66357665839173 22.07994218018414 27.986047827043333 78-79 29.100999748612534 21.478158296753232 21.636725774950204 27.784116179684027 80-81 29.17777670044117 21.459737237601203 21.61972172395404 27.74276433800359 82-83 29.654379312488345 21.632373966556848 21.181699508920246 27.531547212034564 84-85 30.764387356756078 20.9755278512362 19.972295680644756 28.287789111362972 86-87 31.226698737221888 20.396873120865905 19.30847865303668 29.067949488875527 88-89 31.228805106722525 20.885697187312985 18.91083183722322 28.974665868741273 90-91 30.322111891499155 18.890772584160814 19.520465003632843 31.266650520707195 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 2.5 12 3.0 13 5.5 14 14.0 15 21.0 16 24.5 17 32.5 18 46.5 19 62.5 20 89.0 21 135.0 22 206.0 23 317.0 24 477.0 25 646.0 26 861.5 27 1187.0 28 1593.0 29 2082.0 30 2596.5 31 3230.5 32 4108.5 33 5007.0 34 6082.5 35 7429.0 36 8941.0 37 10502.5 38 12255.0 39 14394.5 40 16566.5 41 18779.5 42 21010.0 43 23183.0 44 25423.0 45 27740.0 46 30059.0 47 32477.0 48 34631.5 49 36658.5 50 39063.0 51 41207.5 52 42854.0 53 44710.0 54 46670.5 55 48326.5 56 49820.5 57 51077.5 58 52112.5 59 52561.5 60 52582.0 61 52214.0 62 51390.5 63 50125.0 64 47749.5 65 44656.0 66 40857.5 67 37127.5 68 33549.0 69 29491.0 70 25401.0 71 21559.5 72 18135.0 73 14848.5 74 11741.0 75 9402.5 76 7486.0 77 5625.0 78 4360.5 79 3362.0 80 2458.0 81 1784.0 82 1300.5 83 926.5 84 669.5 85 506.0 86 360.0 87 255.0 88 189.5 89 119.0 90 67.0 91 52.5 92 36.5 93 21.5 94 17.5 95 14.5 96 9.0 97 6.0 98 3.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.008129039116310917 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.055917350742335455 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 9.221774453840408E-4 64-65 5.4183215123619E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 16756.0 32-33 36465.0 34-35 38186.0 36-37 39854.0 38-39 42047.0 40-41 42845.0 42-43 42996.0 44-45 40960.0 46-47 37470.0 48-49 35391.0 50-51 33288.0 52-53 30113.0 54-55 26980.0 56-57 23683.0 58-59 21117.0 60-61 18722.0 62-63 16765.0 64-65 14445.0 66-67 12573.0 68-69 10870.0 70-71 9376.0 72-73 8306.0 74-75 7132.0 76-77 6047.0 78-79 5428.0 80-81 4723.0 82-83 4272.0 84-85 3707.0 86-87 3377.0 88-89 3017.0 90-91 2771.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.96782163075419 #Duplication Level Percentage of deduplicated Percentage of total 1 99.96898557258359 99.93681718327475 2 0.029840127779557652 0.059661051426114636 3 0.001174299636849723 0.0035217652991295758 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0028138981556460867 0.0 0.0 0.0 0.0 2 0.0028138981556460867 0.0 0.0 0.0 1.563276753136715E-4 3 0.0028138981556460867 0.0 0.0 0.0 1.563276753136715E-4 4 0.002970225830959758 0.0 0.0 0.0 1.563276753136715E-4 5 0.002970225830959758 0.0 0.0 0.0 1.563276753136715E-4 6 0.0031265535062734297 0.0 0.0 0.0 1.563276753136715E-4 7 0.0035955365322144443 0.0 0.0 0.0 1.563276753136715E-4 8 0.012975197051034733 0.0 0.0 0.0 1.563276753136715E-4 9 0.013444180076975747 0.0 0.0 0.0 1.563276753136715E-4 10-11 0.013444180076975747 0.0 0.0 0.0 1.563276753136715E-4 12-13 0.013444180076975747 7.816383765683574E-5 0.0 0.0 1.563276753136715E-4 14-15 0.013444180076975747 1.563276753136715E-4 0.0 0.0 1.563276753136715E-4 16-17 0.013444180076975747 1.563276753136715E-4 0.0 0.0 1.563276753136715E-4 18-19 0.01360050775228942 1.563276753136715E-4 0.0 0.0 2.3449151297050725E-4 20-21 0.013678671589946255 1.563276753136715E-4 0.0 0.0 4.6898302594101444E-4 22-23 0.013913163102916761 1.563276753136715E-4 0.0 0.0 4.6898302594101444E-4 24-25 0.013913163102916761 1.563276753136715E-4 0.0 0.0 4.6898302594101444E-4 26-27 0.014225818453544106 1.563276753136715E-4 0.0 0.0 4.6898302594101444E-4 28-29 0.014460309966514612 1.563276753136715E-4 0.0 0.0 4.6898302594101444E-4 30-31 0.014929292992455625 1.563276753136715E-4 0.0 0.0 5.471468635978502E-4 32-33 0.015632767531367147 1.563276753136715E-4 0.0 0.0 0.0010161298895388646 34-35 0.016414405907935505 1.563276753136715E-4 0.0 0.0 0.0010942937271957004 36-37 0.016727061258562846 1.563276753136715E-4 0.0 0.0 0.0010942937271957004 38-39 0.017430535797474368 1.563276753136715E-4 0.0 0.0 0.001250621402509372 40-41 0.01813401033638589 1.563276753136715E-4 0.0 0.0 0.001250621402509372 42-43 0.018290338011699565 1.563276753136715E-4 0.0 0.0 0.001250621402509372 44-45 0.018759321037640577 1.563276753136715E-4 0.0 0.0 0.001250621402509372 46-47 0.01891564871295425 1.563276753136715E-4 0.0 0.0 0.001250621402509372 48-49 0.019071976388267922 1.563276753136715E-4 0.0 0.0 0.001250621402509372 50-51 0.019071976388267922 1.563276753136715E-4 0.0 0.0 0.001250621402509372 52-53 0.019071976388267922 1.563276753136715E-4 0.0 0.0 0.001250621402509372 54-55 0.019150140225924758 1.563276753136715E-4 0.0 0.0 0.0013287852401662075 56-57 0.0194627955765521 1.563276753136715E-4 0.0 0.0 0.0014069490778230434 58-59 0.019540959414208935 1.563276753136715E-4 0.0 0.0 0.0014069490778230434 60-61 0.019697287089522605 1.563276753136715E-4 0.0 0.0 0.0014069490778230434 62-63 0.019697287089522605 1.563276753136715E-4 0.0 0.0 0.0014069490778230434 64-65 0.019697287089522605 1.563276753136715E-4 0.0 0.0 0.0014069490778230434 66-67 0.01985361476483628 1.563276753136715E-4 0.0 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