##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-32A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 755280 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.5437347738587 40.0 40.0 40.0 40.0 40.0 2 39.347161317657026 40.0 40.0 40.0 40.0 40.0 3 39.283304205063025 40.0 40.0 40.0 40.0 40.0 4 39.173826925113865 40.0 40.0 40.0 40.0 40.0 5 39.22516152949899 40.0 40.0 40.0 40.0 40.0 6 39.34201885393497 40.0 40.0 40.0 40.0 40.0 7 39.35989831585637 40.0 40.0 40.0 40.0 40.0 8 39.358659040355896 40.0 40.0 40.0 40.0 40.0 9 39.375039720368605 40.0 40.0 40.0 40.0 40.0 10-11 39.37147283126788 40.0 40.0 40.0 40.0 40.0 12-13 39.28286992903294 40.0 40.0 40.0 40.0 40.0 14-15 39.32109681177841 40.0 40.0 40.0 40.0 40.0 16-17 39.432107297955724 40.0 40.0 40.0 40.0 40.0 18-19 39.46646276877449 40.0 40.0 40.0 40.0 40.0 20-21 39.44335875436924 40.0 40.0 40.0 40.0 40.0 22-23 39.488875648766026 40.0 40.0 40.0 40.0 40.0 24-25 39.46578752250821 40.0 40.0 40.0 40.0 40.0 26-27 39.4305211312361 40.0 40.0 40.0 40.0 40.0 28-29 39.45229848532995 40.0 40.0 40.0 40.0 40.0 30-31 39.419359836015474 40.0 40.0 40.0 40.0 40.0 32-33 39.4270683180369 40.0 40.0 40.0 40.0 40.0 34-35 39.41941988674246 40.0 40.0 40.0 40.0 40.0 36-37 39.40194828901015 40.0 40.0 40.0 40.0 40.0 38-39 39.3381408767015 40.0 40.0 40.0 40.0 40.0 40-41 39.38451408963291 40.0 40.0 40.0 40.0 40.0 42-43 39.36417251961841 40.0 40.0 40.0 40.0 40.0 44-45 39.25292439654179 40.0 40.0 40.0 40.0 40.0 46-47 39.23211965967576 40.0 40.0 40.0 40.0 40.0 48-49 39.323147293457176 40.0 40.0 40.0 40.0 40.0 50-51 39.27386853200571 40.0 40.0 40.0 40.0 40.0 52-53 39.23615348677245 40.0 40.0 40.0 40.0 40.0 54-55 39.14279326996625 40.0 40.0 40.0 40.0 40.0 56-57 39.16490099793053 40.0 40.0 40.0 40.0 40.0 58-59 39.10907235477829 40.0 40.0 40.0 40.0 40.0 60-61 39.025150944254136 40.0 40.0 40.0 40.0 40.0 62-63 39.39131208566188 40.0 40.0 40.0 40.0 40.0 64-65 39.39006019547457 40.0 40.0 40.0 40.0 40.0 66-67 39.39177582001867 40.0 40.0 40.0 40.0 40.0 68-69 39.395135608316934 40.0 40.0 40.0 40.0 40.0 70-71 39.3896968250408 40.0 40.0 40.0 40.0 40.0 72-73 39.384022107439314 40.0 40.0 40.0 40.0 40.0 74-75 39.37600849528852 40.0 40.0 40.0 40.0 40.0 76-77 39.37476756445941 40.0 40.0 40.0 40.0 40.0 78-79 39.37704315263936 40.0 40.0 40.0 40.0 40.0 80-81 39.35896925789453 40.0 40.0 40.0 40.0 40.0 82-83 39.35197201426881 40.0 40.0 40.0 40.0 40.0 84-85 39.3581885864579 40.0 40.0 40.0 40.0 40.0 86-87 39.337190835217505 40.0 40.0 40.0 40.0 40.0 88-89 39.39205795877254 40.0 40.0 40.0 40.0 40.0 90-91 39.442570176465125 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05675471949385269 1101 2 0.05017891827121446 1101 3 -0.024602870318581438 1101 4 -0.024798277060092744 1101 5 -0.009489188238958945 1101 6 0.07722678556839924 1101 7 -0.006256927170809945 1101 8 0.01005972675693556 1101 9 0.037485158753057135 1101 10-11 0.0580686084063089 1101 12-13 -0.02503929010637762 1101 14-15 0.05560607379960203 1101 16-17 0.04662596197589863 1101 18-19 -0.04065407556135625 1101 20-21 0.024197345872806864 1101 22-23 -0.04257682998964896 1101 24-25 -0.019310200770988217 1101 26-27 0.08898819350193321 1101 28-29 0.10137744276045169 1101 30-31 0.05273872297846083 1101 32-33 0.053026996219507794 1101 34-35 0.01692452043229764 1101 36-37 -0.14871201665047096 1101 38-39 -0.01580688317567791 1101 40-41 -0.056658083193887876 1101 42-43 -0.09639665576134604 1101 44-45 -0.03157691711307109 1101 46-47 -0.05384127891151991 1101 48-49 0.01446236376438037 1101 50-51 -0.1187500682331688 1101 52-53 -0.010836124059174779 1101 54-55 -0.10821776330671895 1101 56-57 0.028181278321142145 1101 58-59 -0.024685387485746446 1101 60-61 0.12278200043689225 1101 62-63 -0.008935607123923717 1101 64-65 -0.16476664362078708 1101 66-67 0.04037515905777411 1101 68-69 -0.17264252682123526 1101 70-71 -0.06432467053576119 1101 72-73 -0.10714275398535023 1101 74-75 -0.2111324827981491 1101 76-77 -0.6278137802526658 1101 78-79 -1.0017591728887751 1101 80-81 0.24613062137329678 1101 82-83 0.08873983329999646 1101 84-85 -0.47975296519648936 1101 86-87 -0.5777283188300402 1101 88-89 -0.9577613631573385 1101 90-91 0.37196601037439336 1102 1 0.07108773605466467 1102 2 -0.07907706599966247 1102 3 -0.009611482933948423 1102 4 0.007832627467529107 1102 5 -0.05252267317909798 1102 6 0.028481378332429585 1102 7 -0.014286651188612609 1102 8 0.016894764376480964 1102 9 -0.11557508963986152 1102 10-11 -0.14424217384489424 1102 12-13 -0.02413776984040794 1102 14-15 -0.015282322808765514 1102 16-17 -0.08148075600171722 1102 18-19 -0.14002638347388086 1102 20-21 -0.09434912818508678 1102 22-23 -0.07337716664897442 1102 24-25 -0.015119163809345082 1102 26-27 -0.09380845999978504 1102 28-29 -0.0803896883612012 1102 30-31 0.0017154286582439227 1102 32-33 -0.03740659857241013 1102 34-35 0.07190329206935786 1102 36-37 -0.018507680621162592 1102 38-39 -0.002141179155323414 1102 40-41 -0.01702012358899907 1102 42-43 -0.003195191965069455 1102 44-45 -0.08402494300460717 1102 46-47 -0.08778016436272651 1102 48-49 -0.018657215332986254 1102 50-51 -0.07763319683302683 1102 52-53 0.09563783332624354 1102 54-55 -0.14015019215519686 1102 56-57 -0.08094789751298492 1102 58-59 -0.015848482759011517 1102 60-61 -0.0562444282118264 1102 62-63 0.06077571703077922 1102 64-65 -0.01942148992826276 1102 66-67 -0.26234683530153546 1102 68-69 -0.15900926099060086 1102 70-71 0.10779603180996133 1102 72-73 0.009900557585019953 1102 74-75 0.20322815424636076 1102 76-77 0.1257097427957916 1102 78-79 0.015802433545793804 1102 80-81 0.5075811769496923 1102 82-83 0.5287029890526327 1102 84-85 0.02339467955666663 1102 86-87 0.1390970779953591 1102 88-89 0.301497896101921 1102 90-91 0.37196601037439336 1103 1 0.03813217407034131 1103 2 0.06729122307073965 1103 3 0.07213395312510329 1103 4 0.1245617968282815 1103 5 0.01161180115613547 1103 6 0.006089727074794382 1103 7 -0.019764135776242142 1103 8 -0.030175341293734448 1103 9 -0.024279828512803192 1103 10-11 -0.01352866548624121 1103 12-13 0.01005451448752126 1103 14-15 -0.07641453478199622 1103 16-17 -0.060771989226758194 1103 18-19 -0.0954986021040014 1103 20-21 -0.037155941312029483 1103 22-23 -0.05253108503240611 1103 24-25 0.08867174790343313 1103 26-27 0.0340085495318192 1103 28-29 -0.04494112876722767 1103 30-31 0.02261705834988703 1103 32-33 0.01415354156335269 1103 34-35 0.11888643454739878 1103 36-37 0.015538745610847116 1103 38-39 0.08710613721874694 1103 40-41 0.08084722886193418 1103 42-43 0.12921887320225522 1103 44-45 0.005453534985051078 1103 46-47 0.002183939312274674 1103 48-49 0.02699642264876445 1103 50-51 0.06936544215869134 1103 52-53 0.045339394853321835 1103 54-55 -0.05884354586824969 1103 56-57 0.011851125135088125 1103 58-59 0.052674356359091234 1103 60-61 0.28534969408804756 1103 62-63 -0.00150203615768163 1103 64-65 -0.08466200539003665 1103 66-67 0.03284153560952063 1103 68-69 0.21561432727830265 1103 70-71 0.19888828156116034 1103 72-73 -0.13782568683591023 1103 74-75 -0.07196852771014761 1103 76-77 -0.016208224773095026 1103 78-79 -0.40553710392483566 1103 80-81 -0.1252029880378629 1103 82-83 0.2512803597877564 1103 84-85 0.413507673906949 1103 86-87 -0.09358266056673159 1103 88-89 0.3397296762691866 1103 90-91 -0.9280339896256038 1104 1 0.01211752381505704 1104 2 -0.025930974779662108 1104 3 -0.03037795269987953 1104 4 -0.06768443982128503 1104 5 -0.020964165940178248 1104 6 -0.0814268647858043 1104 7 0.12936710529926643 1104 8 0.06914780616163085 1104 9 0.04164371931467059 1104 10-11 0.06674689594937888 1104 12-13 0.028687683296652722 1104 14-15 -0.01935587393912641 1104 16-17 -0.0545019782074192 1104 18-19 -0.009551246961450488 1104 20-21 0.0055706464997697935 1104 22-23 0.018532796773726545 1104 24-25 0.019385178402281156 1104 26-27 0.015134359834505062 1104 28-29 0.0015585630208150292 1104 30-31 0.04656309047848595 1104 32-33 0.06644839468231822 1104 34-35 0.024667190231369318 1104 36-37 -0.10374167887152197 1104 38-39 -0.08683926139872966 1104 40-41 -0.03653420190108392 1104 42-43 0.017232668238769122 1104 44-45 -0.051266566361270804 1104 46-47 -0.1276375369219238 1104 48-49 0.04636226095470164 1104 50-51 0.13491787983789294 1104 52-53 -0.0027991558442508335 1104 54-55 -0.1775545256199038 1104 56-57 0.08146931749330122 1104 58-59 0.08516678929435528 1104 60-61 -0.05749260783536414 1104 62-63 -0.10953448742213823 1104 64-65 -0.08746975162724624 1104 66-67 -0.035759472948683424 1104 68-69 -0.15066767559106609 1104 70-71 0.07726899646309704 1104 72-73 -0.18104616680701469 1104 74-75 -0.02817661679752348 1104 76-77 -0.008962119476628061 1104 78-79 0.1120291663501618 1104 80-81 0.05591561519239008 1104 82-83 0.32328174363138373 1104 84-85 0.03951860286869646 1104 86-87 0.2843962232945074 1104 88-89 0.5977941923982186 1104 90-91 0.37196601037439336 1201 1 0.0334508958530364 1201 2 0.038399553747858306 1201 3 0.12273362296178192 1201 4 0.04703526244895784 1201 5 0.02334354107878056 1201 6 0.07353058074203744 1201 7 -0.07435050393213771 1201 8 -0.0033721690617056765 1201 9 -0.06219627911437442 1201 10-11 -0.05474199190549456 1201 12-13 -0.012138740475627685 1201 14-15 -0.056490557979053335 1201 16-17 0.1222878730941872 1201 18-19 0.07478289305059604 1201 20-21 -0.0024079168209496515 1201 22-23 0.04457689359736605 1201 24-25 2.460179998635681E-4 1201 26-27 0.04042627749436889 1201 28-29 -0.0064157314056743076 1201 30-31 0.06811204167520657 1201 32-33 0.030150733824555687 1201 34-35 0.09914497645929998 1201 36-37 0.016092025685807698 1201 38-39 0.16848378196576874 1201 40-41 0.0797918077731552 1201 42-43 0.0478212206214792 1201 44-45 0.02475151681396426 1201 46-47 0.026442762721629265 1201 48-49 0.21127060539707543 1201 50-51 0.10186110059543552 1201 52-53 0.015465725466860647 1201 54-55 0.017573520941731147 1201 56-57 0.25484985817529093 1201 58-59 0.019150175006330983 1201 60-61 0.36111412257260866 1201 62-63 -0.005382650690435753 1201 64-65 0.03244244703611088 1201 66-67 -0.0938307246816521 1201 68-69 -0.1718012387554495 1201 70-71 -0.17698763463771883 1201 72-73 -0.056634755687689164 1201 74-75 0.13454338920841025 1201 76-77 -0.266602098545178 1201 78-79 -0.07046443175217121 1201 80-81 -0.6034603478971405 1201 82-83 -0.5230454168624377 1201 84-85 -1.0260006667427461 1201 86-87 -0.18939270210732673 1201 88-89 -0.7184451238411 1201 90-91 0.37196601037439336 1202 1 0.13665579926278326 1202 2 -0.03821396045945136 1202 3 -0.11254969241361579 1202 4 -0.16824954876214093 1202 5 -0.1633565828772845 1202 6 -0.019740594223968344 1202 7 -0.006403353057272909 1202 8 0.07289993250338256 1202 9 0.10866350525681412 1202 10-11 -0.061922434306438845 1202 12-13 -0.0454937210373032 1202 14-15 -0.02721313067850417 1202 16-17 -0.09982432880828895 1202 18-19 -0.008411004974362868 1202 20-21 -0.002548878858839032 1202 22-23 -0.051638965407832416 1202 24-25 -0.062168696195293194 1202 26-27 0.0049560042112233305 1202 28-29 -0.011249824044462287 1202 30-31 0.07196286517921635 1202 32-33 0.019663146271362564 1202 34-35 -0.013873782635641874 1202 36-37 -0.18207470926330416 1202 38-39 -0.13958533694384556 1202 40-41 -0.13848451329624112 1202 42-43 -0.20618576037688996 1202 44-45 -0.08564666542889654 1202 46-47 -0.06124677831556369 1202 48-49 -0.0015957869369742639 1202 50-51 -0.042026503974220475 1202 52-53 0.09541913038423644 1202 54-55 0.0063120210927110065 1202 56-57 0.13909659270173336 1202 58-59 0.09642936620520004 1202 60-61 -0.017803268419314122 1202 62-63 -0.16506884017834977 1202 64-65 -0.17876338332179387 1202 66-67 -0.22288674923879626 1202 68-69 -0.32100756934359254 1202 70-71 -0.3879114893953215 1202 72-73 -0.17645429326044137 1202 74-75 0.0038372648432201117 1202 76-77 -0.39294266366334796 1202 78-79 -0.2262891287685065 1202 80-81 -0.10112094771844937 1202 82-83 -0.3445260937189971 1202 84-85 -0.17664123789144526 1202 86-87 -0.4158546128258962 1202 88-89 -0.37547769700270095 1202 90-91 -0.4280339896256038 1203 1 0.05356883210916408 1203 2 0.02334114168170487 1203 3 0.0947532696302531 1203 4 -0.0844535645416542 1203 5 -0.16352564290782823 1203 6 -0.1649752361768222 1203 7 0.04905092461433469 1203 8 -0.012902345753040834 1203 9 -0.05882581959419042 1203 10-11 -0.03842999047038376 1203 12-13 -0.006019929007884173 1203 14-15 -0.040213070731198286 1203 16-17 0.04452059160627897 1203 18-19 0.11150215337320901 1203 20-21 -0.10700166992349835 1203 22-23 -0.01103781899107048 1203 24-25 0.04894212739876025 1203 26-27 0.007861076572794445 1203 28-29 0.014566763935974336 1203 30-31 -0.10664223648612392 1203 32-33 0.04008425685753281 1203 34-35 -0.013666088992074776 1203 36-37 -0.015052839262189366 1203 38-39 0.05088567405183397 1203 40-41 -0.00115385654972755 1203 42-43 0.11426238153398316 1203 44-45 -0.037240083415753134 1203 46-47 0.16369345801706459 1203 48-49 -0.09463026728552393 1203 50-51 0.037345576500285915 1203 52-53 0.03191683130634004 1203 54-55 0.00823707670748064 1203 56-57 -0.051606761318382155 1203 58-59 -0.02754623824766611 1203 60-61 -0.1815822609380504 1203 62-63 -7.428111853968744E-4 1203 64-65 0.039068580617161786 1203 66-67 -0.1978477092456501 1203 68-69 -0.07843119691428058 1203 70-71 -0.18857996968748836 1203 72-73 -0.15695320788981348 1203 74-75 -0.3539690592674489 1203 76-77 -0.1489123542015065 1203 78-79 0.16435612341520311 1203 80-81 -0.29848566942396104 1203 82-83 -0.434586594926607 1203 84-85 0.20166304239018018 1203 86-87 0.07041020930849129 1203 88-89 0.035369949973976134 1203 90-91 0.37196601037439336 1204 1 0.025068495137908542 1204 2 -0.08051006821167306 1204 3 -0.19966564927712938 1204 4 -0.16204151660204502 1204 5 -0.09311957759605605 1204 6 -0.053674783379705104 1204 7 -0.11300107231405576 1204 8 -0.14731842880598833 1204 9 -0.0860158551534127 1204 10-11 -0.15989510220048686 1204 12-13 -0.2645593102278667 1204 14-15 -0.22749648448492366 1204 16-17 -0.16643537321204604 1204 18-19 -0.09188242179956774 1204 20-21 -0.098037124083028 1204 22-23 -0.020320117194827958 1204 24-25 -0.12122536738864653 1204 26-27 -0.06534667624319468 1204 28-29 -0.15107788471276962 1204 30-31 -0.09235142343629121 1204 32-33 -0.08735518740790127 1204 34-35 -0.09511126685118398 1204 36-37 -0.08249199769834092 1204 38-39 -0.12113163599222077 1204 40-41 -0.04739116881987826 1204 42-43 -0.032787726864278 1204 44-45 -0.18085655433642955 1204 46-47 -0.10500907661991477 1204 48-49 -0.15650425982946814 1204 50-51 -0.11172098428058774 1204 52-53 -0.168497437494878 1204 54-55 0.048003431707094535 1204 56-57 -0.14830474200169874 1204 58-59 -0.191858578434136 1204 60-61 -0.319574762883299 1204 62-63 -0.24807975782582758 1204 64-65 -0.3150305945866805 1204 66-67 -0.11242883885523725 1204 68-69 -0.10774292973748345 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2401 32-33 -0.01905137916426014 2401 34-35 0.00999250338804103 2401 36-37 0.04690781656059073 2401 38-39 0.1295078936017049 2401 40-41 0.12709435103450772 2401 42-43 0.10736752926995763 2401 44-45 0.08744878452357341 2401 46-47 0.1765641172481054 2401 48-49 -0.0484873705697737 2401 50-51 0.10492736131946145 2401 52-53 0.018509115921396813 2401 54-55 0.0892611993402781 2401 56-57 0.05007463692366798 2401 58-59 0.01935353946947771 2401 60-61 0.0786827316734886 2401 62-63 0.11835928887789038 2401 64-65 0.15317728664278007 2401 66-67 -0.0016589055819622445 2401 68-69 0.09348094883982583 2401 70-71 0.15887526531535912 2401 72-73 0.08847795753410281 2401 74-75 -0.13316739716143644 2401 76-77 -0.2731642960609051 2401 78-79 0.15898223983308668 2401 80-81 0.18521726635049163 2401 82-83 0.008866892945349036 2401 84-85 -0.41553313160444816 2401 86-87 -0.23505258186858669 2401 88-89 0.16029419239821863 2401 90-91 0.37196601037439336 2402 1 0.03600988037776176 2402 2 0.12891711728192234 2402 3 0.10262173252910856 2402 4 0.15346899661215474 2402 5 0.08363052858761932 2402 6 0.13775409830351037 2402 7 0.2131924115137238 2402 8 0.12338876130607446 2402 9 0.0356053392444835 2402 10-11 0.14243763961993494 2402 12-13 0.12976079126860895 2402 14-15 0.07280192942658203 2402 16-17 0.06324780770705729 2402 18-19 0.13670455877672794 2402 20-21 0.0810936955724273 2402 22-23 0.04319146266698226 2402 24-25 0.029673654347043055 2402 26-27 0.06476440080972168 2402 28-29 0.10790649082416337 2402 30-31 0.06879087197518174 2402 32-33 0.04434064051105935 2402 34-35 0.08625880069379122 2402 36-37 0.10673714513656307 2402 38-39 -0.15801676101214213 2402 40-41 0.062123522844999 2402 42-43 0.05145784412895438 2402 44-45 0.04273723473467328 2402 46-47 0.1281377526610754 2402 48-49 0.010160130554282887 2402 50-51 -0.06112910797618554 2402 52-53 0.08473895332780756 2402 54-55 0.12129421641559901 2402 56-57 0.08429624682658243 2402 58-59 0.11134500961971128 2402 60-61 0.2265443131779321 2402 62-63 0.0845972794424199 2402 64-65 0.0809158571407238 2402 66-67 -0.11725506405673514 2402 68-69 0.15743959906558302 2402 70-71 0.15638570884076586 2402 72-73 0.0083051836023742 2402 74-75 0.2860454006227897 2402 76-77 0.24454313925706828 2402 78-79 0.2597987176308081 2402 80-81 0.2766701524902828 2402 82-83 0.38804364839329253 2402 84-85 0.2572913637000269 2402 86-87 -0.05293061027293078 2402 88-89 0.5977941923982186 2402 90-91 0.37196601037439336 2403 1 -0.004772757554583507 2403 2 0.05419016204282201 2403 3 0.09460229673123166 2403 4 0.07811189636377946 2403 5 0.09586466921516035 2403 6 0.11800593938983184 2403 7 -0.06549437062530217 2403 8 0.09592485093634195 2403 9 0.14833730527521993 2403 10-11 0.08572567337228065 2403 12-13 0.09144992696127474 2403 14-15 0.06165454490797373 2403 16-17 0.03775242262446454 2403 18-19 0.09633948681438653 2403 20-21 0.06152991643771344 2403 22-23 0.054219542622725214 2403 24-25 0.038341673132087806 2403 26-27 -0.010939767257866606 2403 28-29 0.0396599425220856 2403 30-31 0.05668145884517628 2403 32-33 0.015014973828748168 2403 34-35 -0.006119231075437881 2403 36-37 0.06756397795479074 2403 38-39 0.05707613811589596 2403 40-41 0.021941831919662036 2403 42-43 0.12450368757462371 2403 44-45 0.08374424528058455 2403 46-47 0.042771899528702306 2403 48-49 0.09705891125311439 2403 50-51 0.027796438956400493 2403 52-53 0.0574054107739741 2403 54-55 0.061287402561269744 2403 56-57 0.11364413241943794 2403 58-59 -0.08224221943109455 2403 60-61 0.039705994558921986 2403 62-63 -0.03901770726660203 2403 64-65 0.17986933378282544 2403 66-67 0.023499529176888245 2403 68-69 0.08091900060502155 2403 70-71 0.11039404949749354 2403 72-73 0.15965094047194128 2403 74-75 0.05446895890145953 2403 76-77 -0.029126894093607802 2403 78-79 -0.2596867526175828 2403 80-81 0.31342145132500576 2403 82-83 0.20290079125043547 2403 84-85 0.1526839774167854 2403 86-87 -0.22958979069150587 2403 88-89 0.11351746283846609 2403 90-91 0.37196601037439336 2404 1 0.019291287653800282 2404 2 -0.048305717401490256 2404 3 0.0012972186368926941 2404 4 0.043232373563370174 2404 5 0.06371648618399917 2404 6 0.03196873665849154 2404 7 0.04604056907069776 2404 8 0.11282566981502384 2404 9 0.04670739658150325 2404 10-11 0.04858834767832576 2404 12-13 0.10870916877902914 2404 14-15 0.08217098788733779 2404 16-17 0.06299376945752755 2404 18-19 0.06918044644601196 2404 20-21 0.08457886602304399 2404 22-23 -0.019403668532859797 2404 24-25 0.0363010169826552 2404 26-27 0.11070165282951194 2404 28-29 0.020051329906642934 2404 30-31 0.01741975777513005 2404 32-33 -0.016716840248840015 2404 34-35 0.09024757822552232 2404 36-37 0.08241393838424926 2404 38-39 0.22525169391420974 2404 40-41 0.014622365321450559 2404 42-43 0.02538286386296562 2404 44-45 0.07491606664212469 2404 46-47 0.05283368688503032 2404 48-49 0.003214236136791726 2404 50-51 0.05836393790261951 2404 52-53 0.09392671399881891 2404 54-55 -0.04329305140979045 2404 56-57 0.023875433135636115 2404 58-59 0.05616583483528359 2404 60-61 0.10339889921039713 2404 62-63 -0.06666953317058955 2404 64-65 0.11418259942346509 2404 66-67 0.15435038651411048 2404 68-69 0.2705641301667683 2404 70-71 0.2627990517017409 2404 72-73 0.18375512084055146 2404 74-75 0.30358515871974845 2404 76-77 -0.017586422278313307 2404 78-79 0.2608019731629412 2404 80-81 0.05085608386097107 2404 82-83 -0.4325543582512239 2404 84-85 0.11621037660965072 2404 86-87 0.371420310318598 2404 88-89 0.3477941923982186 2404 90-91 0.37196601037439336 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 24.0 31 525.0 32 3260.0 33 6054.0 34 8235.0 35 9962.0 36 14563.0 37 21852.0 38 54867.0 39 299924.0 40 336014.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.85176358436606 13.726432581294354 29.58849698125199 29.8333068530876 2 27.36032946224029 16.960551928704795 28.105062436272625 27.574056172782285 3 27.742198578273726 19.085000377344002 21.742296555312674 31.430504489069598 4 27.76467005613812 20.910523249655757 20.261492426649717 31.063314267556404 5 27.3731596229213 20.540064611799597 20.791097341383328 31.295678423895772 6 28.389339053066415 22.7648024573668 22.97995445397733 25.86590403558945 7 26.638200402499734 22.638094481516788 22.8581453235886 27.865559792394873 8 25.452017794725133 23.524520707552167 23.224102319669527 27.799359178053173 9 25.878482152314376 23.26196907107298 23.217482258235357 27.64206651837729 10-11 25.479755852134307 22.51277671856795 23.311222328143206 28.69624510115454 12-13 26.264299332697803 22.39652843978392 22.64120591039085 28.69796631712742 14-15 26.33712000847368 21.969335875436926 22.7341515729266 28.9593925431628 16-17 26.43853934964516 21.752131659781803 22.583147971613176 29.226181018959856 18-19 26.774242664971933 21.993631500900328 22.31788210994598 28.91424372418176 20-21 26.617943014511177 21.995948522402287 22.33701408749073 29.049094375595807 22-23 26.451514670056138 21.85156498252304 22.493115136108464 29.20380521131236 24-25 26.73988454612859 22.06526056561805 22.390768986336195 28.80408590191717 26-27 26.7389577375278 22.05288105073615 22.43889683296261 28.769264378773435 28-29 26.668123080182184 21.923326448469442 22.61512286834022 28.793427603008155 30-31 26.996074879826708 22.02350570363853 22.46878076327004 28.511638653264722 32-33 26.94858761939788 22.034945653614788 22.5819104611948 28.434556265792537 34-35 26.75177248664123 21.902711162111917 22.860275673679592 28.485240677567266 36-37 27.017774607279264 21.98232942470314 22.766507646350707 28.23338832166689 38-39 27.262133879221466 21.921821469767675 22.734057781536386 28.081986869474473 40-41 26.900185025163086 21.882192289113934 22.89782499327257 28.319797692450404 42-43 27.127452802324132 21.99879879232843 22.876721798328184 27.997026607019254 44-45 27.138002826500678 21.945522795708538 22.937227968749088 27.979246409041693 46-47 27.035847498225973 21.856218716588284 23.135209906206608 27.97272387897913 48-49 27.32124280984907 22.049101889255187 22.93234344245507 27.697311858440663 50-51 27.396463960776963 22.001766872291846 22.938617122853717 27.66315204407747 52-53 27.15930399586756 21.90516625271545 23.152114689253544 27.783415062163442 54-55 27.363062313378038 22.09414510365562 23.09319926290741 27.44959332005893 56-57 27.44723933227396 22.003851311731264 23.088560682961223 27.46034867303355 58-59 27.271911027043856 21.94155463241192 23.325671507184957 27.460862833359272 60-61 27.784750124147894 22.05114893232811 23.02491986817751 27.139181075346485 62-63 27.570666597954087 22.089721456363215 23.01759901053879 27.322012935143906 64-65 27.57881440364361 21.921849795995826 23.197765442641614 27.301570357718948 66-67 27.719935210223607 22.10573636600733 22.958161178295423 27.21616724547364 68-69 28.026452489411014 21.86086470071126 22.95492687604891 27.15775593382882 70-71 27.62795584930974 22.16775573579011 23.0735672838319 27.13072113106826 72-73 27.908952116585706 22.146287300485774 22.856904927133932 27.087855655794584 74-75 28.05657419217687 22.0703125 22.882121598639458 26.990991709183675 76-77 27.874258255933952 21.95965557275542 23.02873452012384 27.13735165118679 78-79 28.07822690958111 21.876177071149613 22.83171104018397 27.213884979085307 80-81 28.434986177869646 21.91617064727418 22.334570268722135 27.314272906134036 82-83 28.65727911323065 21.69717084487981 22.29812463749436 27.34742540439518 84-85 29.62971030276628 21.265519494663472 21.727292528860815 27.377477673709432 86-87 29.806504734458628 20.48959685847294 21.572663647591604 28.131234759476836 88-89 29.847667407172324 19.692161218660743 21.395324235692375 29.06484713847456 90-91 27.82404997397189 19.000520562207186 22.17595002602811 30.999479437792814 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.5 9 3.0 10 4.5 11 8.5 12 12.0 13 15.5 14 24.0 15 42.5 16 65.0 17 83.0 18 123.5 19 184.0 20 250.5 21 334.5 22 453.0 23 648.0 24 914.0 25 1176.5 26 1483.0 27 1938.5 28 2556.0 29 3235.0 30 3957.5 31 4842.0 32 5869.0 33 6868.5 34 8097.5 35 9659.0 36 11391.5 37 13151.0 38 15033.0 39 17273.0 40 19506.0 41 21736.0 42 24109.5 43 26512.0 44 28981.0 45 31424.5 46 33825.5 47 36432.0 48 38873.5 49 41074.5 50 43225.0 51 45404.5 52 47412.0 53 49178.5 54 51165.5 55 53044.0 56 54688.5 57 55836.5 58 56614.5 59 57253.0 60 57138.0 61 56390.0 62 55288.0 63 53601.5 64 50892.0 65 47497.5 66 43576.5 67 39723.5 68 35735.5 69 31128.5 70 26594.5 71 22467.5 72 18769.0 73 15162.5 74 11944.5 75 9539.5 76 7555.5 77 5703.0 78 4332.5 79 3308.0 80 2409.0 81 1718.5 82 1247.5 83 887.5 84 644.5 85 475.0 86 329.5 87 229.0 88 166.0 89 117.0 90 81.5 91 56.5 92 37.5 93 28.0 94 20.5 95 13.5 96 8.5 97 4.5 98 3.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01456413515517424 3 1.3240122868340217E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.058778751755907306 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 5.153417230965853E-4 64-65 0.0 66-67 3.4316501089548914E-4 68-69 0.0 70-71 0.0 72-73 5.551669386984666E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0021781272461937227 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 12807.0 32-33 29149.0 34-35 31926.0 36-37 34946.0 38-39 38149.0 40-41 40692.0 42-43 42539.0 44-45 42160.0 46-47 40623.0 48-49 40154.0 50-51 39431.0 52-53 37022.0 54-55 35296.0 56-57 32244.0 58-59 29549.0 60-61 27860.0 62-63 26065.0 64-65 23476.0 66-67 21202.0 68-69 19094.0 70-71 16821.0 72-73 15352.0 74-75 13600.0 76-77 11909.0 78-79 10665.0 80-81 9412.0 82-83 8233.0 84-85 7475.0 86-87 6471.0 88-89 5772.0 90-91 5186.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.93107498205066 #Duplication Level Percentage of deduplicated Percentage of total 1 99.93864788768892 99.86976515669399 2 0.055184281860527054 0.11029249216869895 3 0.005346146907955382 0.016027386225718428 4 6.113732117902422E-4 0.002443807290777113 5 0.0 0.0 6 0.0 0.0 7 2.1031033083264406E-4 0.0014711576207955784 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0011916110581506197 0.0 0.0 0.0 0.0 2 0.0013240122868340219 0.0 0.0 0.0 0.0 3 0.001456413515517424 0.0 0.0 0.0 0.0 4 0.001456413515517424 0.0 0.0 0.0 0.0 5 0.001456413515517424 0.0 0.0 0.0 0.0 6 0.0015888147442008262 0.0 0.0 0.0 0.0 7 0.0017212159728842284 0.0 0.0 0.0 0.0 8 0.0067524626628535115 0.0 0.0 0.0 0.0 9 0.007149666348903718 0.0 0.0 0.0 0.0 10-11 0.007149666348903718 0.0 0.0 0.0 0.0 12-13 0.007149666348903718 0.0 0.0 0.0 0.0 14-15 0.007149666348903718 0.0 6.62006143417011E-5 0.0 1.324012286834022E-4 16-17 0.007149666348903718 1.324012286834022E-4 1.324012286834022E-4 0.0 1.324012286834022E-4 18-19 0.007149666348903718 1.324012286834022E-4 1.324012286834022E-4 0.0 1.324012286834022E-4 20-21 0.007149666348903718 2.648024573668044E-4 1.324012286834022E-4 0.0 1.324012286834022E-4 22-23 0.007215866963245419 2.648024573668044E-4 1.324012286834022E-4 0.0 1.324012286834022E-4 24-25 0.0076130706492956255 2.648024573668044E-4 1.324012286834022E-4 0.0 2.648024573668044E-4 26-27 0.008010274335345832 2.648024573668044E-4 1.324012286834022E-4 0.0 2.648024573668044E-4 28-29 0.00853987925007944 3.3100307170850547E-4 1.324012286834022E-4 0.0 2.648024573668044E-4 30-31 0.008870882321787947 3.9720368605020655E-4 1.324012286834022E-4 0.0 2.648024573668044E-4 32-33 0.008870882321787947 3.9720368605020655E-4 1.324012286834022E-4 0.0 2.648024573668044E-4 34-35 0.009334286622179853 5.296049147336088E-4 1.324012286834022E-4 0.0 2.648024573668044E-4 36-37 0.009930092151255164 5.296049147336088E-4 1.324012286834022E-4 0.0 2.648024573668044E-4 38-39 0.01026109522296367 5.296049147336088E-4 1.324012286834022E-4 0.0 3.9720368605020655E-4 40-41 0.010592098294672175 5.296049147336088E-4 1.324012286834022E-4 0.0 3.9720368605020655E-4 42-43 0.010658298909013875 5.296049147336088E-4 1.324012286834022E-4 0.0 3.9720368605020655E-4 44-45 0.010856900752038979 5.296049147336088E-4 1.324012286834022E-4 0.0 3.9720368605020655E-4 46-47 0.011055502595064083 5.296049147336088E-4 1.324012286834022E-4 0.0 3.9720368605020655E-4 48-49 0.011187903823747484 5.296049147336088E-4 1.324012286834022E-4 0.0 3.9720368605020655E-4 50-51 0.011386505666772588 5.296049147336088E-4 1.324012286834022E-4 0.0 4.634043003919077E-4 52-53 0.011386505666772588 6.620061434170109E-4 1.324012286834022E-4 0.0 5.296049147336088E-4 54-55 0.01151890689545599 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