##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-31C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 684109 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.55377871070253 40.0 40.0 40.0 40.0 40.0 2 39.29111296591625 40.0 40.0 40.0 40.0 40.0 3 39.271116152542945 40.0 40.0 40.0 40.0 40.0 4 39.152959542996804 40.0 40.0 40.0 40.0 40.0 5 39.20980428557437 40.0 40.0 40.0 40.0 40.0 6 39.31101037992484 40.0 40.0 40.0 40.0 40.0 7 39.35576348213516 40.0 40.0 40.0 40.0 40.0 8 39.36178591423297 40.0 40.0 40.0 40.0 40.0 9 39.375760295508464 40.0 40.0 40.0 40.0 40.0 10-11 39.37435993387019 40.0 40.0 40.0 40.0 40.0 12-13 39.28009425398584 40.0 40.0 40.0 40.0 40.0 14-15 39.3095603186042 40.0 40.0 40.0 40.0 40.0 16-17 39.417282918365345 40.0 40.0 40.0 40.0 40.0 18-19 39.46298323805125 40.0 40.0 40.0 40.0 40.0 20-21 39.44077917407898 40.0 40.0 40.0 40.0 40.0 22-23 39.48479847509681 40.0 40.0 40.0 40.0 40.0 24-25 39.46001587466325 40.0 40.0 40.0 40.0 40.0 26-27 39.43576535318202 40.0 40.0 40.0 40.0 40.0 28-29 39.455788478151874 40.0 40.0 40.0 40.0 40.0 30-31 39.42973266172899 40.0 40.0 40.0 40.0 40.0 32-33 39.42926911300214 40.0 40.0 40.0 40.0 40.0 34-35 39.435387415890226 40.0 40.0 40.0 40.0 40.0 36-37 39.41490223652577 40.0 40.0 40.0 40.0 40.0 38-39 39.35568917485363 40.0 40.0 40.0 40.0 40.0 40-41 39.40571056686871 40.0 40.0 40.0 40.0 40.0 42-43 39.37850149660463 40.0 40.0 40.0 40.0 40.0 44-45 39.2722157169549 40.0 40.0 40.0 40.0 40.0 46-47 39.27273759079208 40.0 40.0 40.0 40.0 40.0 48-49 39.36148413789458 40.0 40.0 40.0 40.0 40.0 50-51 39.32023579727347 40.0 40.0 40.0 40.0 40.0 52-53 39.282517783763446 40.0 40.0 40.0 40.0 40.0 54-55 39.20391007012627 40.0 40.0 40.0 40.0 40.0 56-57 39.2159047207376 40.0 40.0 40.0 40.0 40.0 58-59 39.16887690537358 40.0 40.0 40.0 40.0 40.0 60-61 39.0956283494345 40.0 40.0 40.0 40.0 40.0 62-63 39.3685571653841 40.0 40.0 40.0 40.0 40.0 64-65 39.37071936516893 40.0 40.0 40.0 40.0 40.0 66-67 39.37642409096816 40.0 40.0 40.0 40.0 40.0 68-69 39.37258297792613 40.0 40.0 40.0 40.0 40.0 70-71 39.36885221754969 40.0 40.0 40.0 40.0 40.0 72-73 39.37175395360923 40.0 40.0 40.0 40.0 40.0 74-75 39.376438327389124 40.0 40.0 40.0 40.0 40.0 76-77 39.37915028576348 40.0 40.0 40.0 40.0 40.0 78-79 39.35641322214317 40.0 40.0 40.0 40.0 40.0 80-81 39.36608500188197 40.0 40.0 40.0 40.0 40.0 82-83 39.34568812083488 40.0 40.0 40.0 40.0 40.0 84-85 39.34041663860444 40.0 40.0 40.0 40.0 40.0 86-87 39.35380032303436 40.0 40.0 40.0 40.0 40.0 88-89 39.38709975982118 40.0 40.0 40.0 40.0 40.0 90-91 39.363137385300305 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04107200867805716 1101 2 0.10487158766522242 1101 3 0.21232554840845097 1101 4 0.12606018162204435 1101 5 0.03739615630973958 1101 6 0.06673847208228523 1101 7 0.0443466211712078 1101 8 0.09451590871171334 1101 9 0.05908629752337191 1101 10-11 0.017205831219563095 1101 12-13 0.081954452580419 1101 14-15 0.021212785462218164 1101 16-17 0.05610674654161585 1101 18-19 -0.01667493245108176 1101 20-21 0.011309495901194566 1101 22-23 0.009772411721762353 1101 24-25 -0.007691147863710057 1101 26-27 0.05706375210242243 1101 28-29 0.021798277256586118 1101 30-31 0.09731365067073483 1101 32-33 0.03973912353796294 1101 34-35 0.052092199096478 1101 36-37 -0.051851741934626716 1101 38-39 0.0769295363758502 1101 40-41 0.03702863022299141 1101 42-43 -0.039347543832626286 1101 44-45 0.041089108497580185 1101 46-47 0.07120231642561237 1101 48-49 0.18585721845588665 1101 50-51 0.03678619580740161 1101 52-53 0.15860755663275228 1101 54-55 0.14328542250402165 1101 56-57 0.10876024972530729 1101 58-59 0.0445975614127363 1101 60-61 0.18372574208411407 1101 62-63 0.27997028930752776 1101 64-65 0.14255784872784005 1101 66-67 0.2790492714430357 1101 68-69 0.09955234795891243 1101 70-71 0.046180267222474924 1101 72-73 -0.06451677210323226 1101 74-75 0.10511978438656655 1101 76-77 0.1001965402563485 1101 78-79 -0.16616675105773737 1101 80-81 0.12268005525336889 1101 82-83 0.08446969667006954 1101 84-85 -0.6664241011853207 1101 86-87 0.1540165710617174 1101 88-89 0.14414595044215872 1101 90-91 0.938936273672148 1102 1 0.07265804999442338 1102 2 -0.15384908718213808 1102 3 0.08791822448633013 1102 4 -0.22808057560820316 1102 5 -0.18919209883145527 1102 6 -0.14440291879512301 1102 7 -0.017557583403139176 1102 8 -0.1272973728946809 1102 9 0.0578627835222818 1102 10-11 -0.08580400738574667 1102 12-13 -0.1735959433833898 1102 14-15 -0.1706127283654979 1102 16-17 -0.04669995138031169 1102 18-19 -0.07413806139550871 1102 20-21 -0.0991178963357413 1102 22-23 -0.05083764660238188 1102 24-25 -0.028312262177919933 1102 26-27 -0.024086281010099242 1102 28-29 -0.06478109923577335 1102 30-31 -0.054076943623748264 1102 32-33 0.047038965584057735 1102 34-35 -0.059933104151035366 1102 36-37 -0.021929112012486485 1102 38-39 0.05746254428458997 1102 40-41 -0.047605458887488794 1102 42-43 -0.06513074822239417 1102 44-45 -0.010560853441276663 1102 46-47 -0.009514414488691614 1102 48-49 -0.19160670188854567 1102 50-51 0.0792901643841688 1102 52-53 0.01780088333600105 1102 54-55 -0.19984496462808465 1102 56-57 0.09292501045590029 1102 58-59 0.05956854964303915 1102 60-61 0.050048813004522685 1102 62-63 -0.05494971453131825 1102 64-65 -0.16356096074189708 1102 66-67 -0.17286615007010653 1102 68-69 -0.17436364473550015 1102 70-71 -0.07166787274649522 1102 72-73 -0.2136960126952161 1102 74-75 -0.2081441861269795 1102 76-77 0.26912162072738965 1102 78-79 0.23505681748585516 1102 80-81 0.4813705382376412 1102 82-83 0.16126616238278757 1102 84-85 -0.2558052194863052 1102 86-87 0.13886505591020182 1102 88-89 -0.04519847419638978 1102 90-91 0.4056029403388166 1103 1 0.0387058280194168 1103 2 0.13781610298945424 1103 3 0.09232853946484454 1103 4 -0.12833612059753818 1103 5 -0.0406576629968356 1103 6 0.10830720000802785 1103 7 -0.09661096901117361 1103 8 -0.03394880406861489 1103 9 -0.006923302840185386 1103 10-11 0.026629397845631786 1103 12-13 -0.00738306900475294 1103 14-15 0.09534924835254799 1103 16-17 -0.03911260628885316 1103 18-19 -0.07303315898650453 1103 20-21 -0.02902049413441432 1103 22-23 -0.025322649962980393 1103 24-25 -0.05989899382198871 1103 26-27 -0.013503151626721888 1103 28-29 -0.0734262925326945 1103 30-31 0.019076658143163172 1103 32-33 -0.023393899748377578 1103 34-35 -0.12740919948510054 1103 36-37 0.040095851762991686 1103 38-39 -0.052826715201163665 1103 40-41 0.03213709246745822 1103 42-43 -0.04187428498835999 1103 44-45 -0.02651607937242062 1103 46-47 0.07315308168164592 1103 48-49 0.04040565459477108 1103 50-51 0.17365384690096874 1103 52-53 0.13980557491274936 1103 54-55 -0.03737133250843527 1103 56-57 0.18677727001543332 1103 58-59 0.10462248720128997 1103 60-61 -0.013999680101719036 1103 62-63 -0.20983559925591777 1103 64-65 0.17417571589333392 1103 66-67 -0.21068974380257544 1103 68-69 -0.290616268245131 1103 70-71 -0.30339761741353044 1103 72-73 -0.16297184705997125 1103 74-75 -0.04490843073278228 1103 76-77 -0.05262845384189063 1103 78-79 0.0801201392572537 1103 80-81 -0.35291420878167656 1103 82-83 0.05967699351916167 1103 84-85 0.4016660448815159 1103 86-87 0.2064326234777667 1103 88-89 -0.4272826209864107 1103 90-91 -4.8943970596611805 1104 1 0.016774024585011205 1104 2 -0.005866644091241824 1104 3 -0.20172572951646117 1104 4 -0.09371154962180839 1104 5 -0.013956683592759589 1104 6 -0.13109703956630625 1104 7 0.04876827049412924 1104 8 0.025134637182326003 1104 9 -0.15272224750261643 1104 10-11 -0.04515951255007877 1104 12-13 0.030433470139655583 1104 14-15 0.020062979061982844 1104 16-17 -0.09913210048891585 1104 18-19 -0.0013872796903910967 1104 20-21 -0.017407362885435873 1104 22-23 -0.10047748527012601 1104 24-25 0.004110460588918841 1104 26-27 0.0421884092622804 1104 28-29 0.050570073704228946 1104 30-31 -0.04243555058284443 1104 32-33 -0.19677343774504408 1104 34-35 0.023952960878553142 1104 36-37 -0.21970980314087996 1104 38-39 -0.04317309659215596 1104 40-41 -0.15865451405036168 1104 42-43 -0.15626803586758342 1104 44-45 -0.18651105211649366 1104 46-47 -0.1507722039736592 1104 48-49 -0.1468517968508607 1104 50-51 0.03940981428468149 1104 52-53 -0.0193864907943464 1104 54-55 -0.13525979272311872 1104 56-57 -0.03412557388367077 1104 58-59 -0.054793981537535785 1104 60-61 0.04901929386059578 1104 62-63 0.04644693218865115 1104 64-65 0.12679119417450835 1104 66-67 0.04891612139073942 1104 68-69 0.08007354856824378 1104 70-71 -0.06303866747938258 1104 72-73 0.07587165548604702 1104 74-75 -0.13601315499985134 1104 76-77 -0.0910241347446572 1104 78-79 0.03133453332878844 1104 80-81 0.006507079431273155 1104 82-83 0.029279746700311193 1104 84-85 -0.6480296954836007 1104 86-87 -1.086778841066284 1104 88-89 -0.04208026418162092 1104 90-91 -0.20392086918499075 1201 1 -0.007996642442456903 1201 2 0.041661430594182036 1201 3 -0.07407317911732747 1201 4 -0.025006367643726435 1201 5 -0.032709220461924815 1201 6 0.11737272095324869 1201 7 0.07430202776648542 1201 8 0.10138954179604553 1201 9 -0.09955488178755445 1201 10-11 0.06613530547183899 1201 12-13 0.053282881586007136 1201 14-15 0.022030236537290193 1201 16-17 -0.029918191680899042 1201 18-19 -0.058554104206699265 1201 20-21 0.01637155742304941 1201 22-23 -0.007964970048909947 1201 24-25 -0.01663368769953877 1201 26-27 0.007613926783591296 1201 28-29 0.039957166113914866 1201 30-31 -0.08975255775371949 1201 32-33 -0.036826119755282605 1201 34-35 -0.06998248950627328 1201 36-37 0.04949592969434491 1201 38-39 0.006534059086369837 1201 40-41 0.010929827866242192 1201 42-43 0.07116754301693362 1201 44-45 0.0697017622552707 1201 46-47 0.08264480835259036 1201 48-49 0.011543763165065002 1201 50-51 0.09701949983303138 1201 52-53 -0.06239047749398452 1201 54-55 -0.05642817414693724 1201 56-57 0.07109633541546145 1201 58-59 -0.061533116563786905 1201 60-61 0.02829771234961953 1201 62-63 -0.23230034252154752 1201 64-65 -0.07678960540958002 1201 66-67 -0.19515244648421515 1201 68-69 -0.13957809503840934 1201 70-71 -0.19629509429255876 1201 72-73 0.3433496209067286 1201 74-75 0.11079705062129364 1201 76-77 0.19460865254926318 1201 78-79 0.3214923187434948 1201 80-81 0.22062684339623928 1201 82-83 0.15836527899938346 1201 84-85 0.39181382813274723 1201 86-87 0.035690452735600786 1201 88-89 0.35666966960724267 1201 90-91 0.36750770224357865 1202 1 -0.012562602797892453 1202 2 -0.02253542518162277 1202 3 0.08584479764275699 1202 4 -0.03679037311194122 1202 5 -0.10945563194518115 1202 6 0.0871061454235118 1202 7 0.11514960124564055 1202 8 -0.06424272067832248 1202 9 0.0012790211516104932 1202 10-11 -0.07610666308591618 1202 12-13 0.024410701134883084 1202 14-15 -0.032638252868039785 1202 16-17 -0.029289346841530062 1202 18-19 -0.10057734412467312 1202 20-21 -0.1272648062133186 1202 22-23 -0.017643712359237895 1202 24-25 -0.048048588588123664 1202 26-27 0.00970551940149278 1202 28-29 -0.10623558849989223 1202 30-31 0.06229118797423183 1202 32-33 0.04716955392829192 1202 34-35 -0.0393655418861556 1202 36-37 -0.1890655989174661 1202 38-39 -0.19437375484011454 1202 40-41 -0.20218830483162975 1202 42-43 -0.07574944299044262 1202 44-45 -0.031425701087890445 1202 46-47 -0.009716652473827025 1202 48-49 -0.18069650599137788 1202 50-51 -0.004985013656721549 1202 52-53 -0.03338507389553769 1202 54-55 -0.15868693329532846 1202 56-57 -0.06378217082364301 1202 58-59 -0.200806766496342 1202 60-61 -0.08516753088569828 1202 62-63 -0.26531161532398073 1202 64-65 -0.40133867495894293 1202 66-67 -0.25053388840100865 1202 68-69 -0.05658201752041947 1202 70-71 0.11699864447849251 1202 72-73 -0.15922569094706773 1202 74-75 -0.42802279689465195 1202 76-77 -0.2122689864092422 1202 78-79 -0.4705841828756405 1202 80-81 -0.42359073344166376 1202 82-83 -0.5682415327492336 1202 84-85 -0.6394830149287998 1202 86-87 -0.5931610878806453 1202 88-89 0.35666966960724267 1202 90-91 0.5579838927197684 1203 1 0.06113173976370234 1203 2 0.11418780033709197 1203 3 -0.09228030636651141 1203 4 -0.05602419925504876 1203 5 -0.1365857749953605 1203 6 0.008722366209390486 1203 7 0.01784299191442784 1203 8 0.016736597287156485 1203 9 -0.049870239633733604 1203 10-11 0.039961953074957535 1203 12-13 -0.10381658157727713 1203 14-15 0.0067543995845795735 1203 16-17 -0.031440757706420186 1203 18-19 -0.03763419808568358 1203 20-21 -0.02486883212077373 1203 22-23 0.0541937070127787 1203 24-25 0.06311704741605695 1203 26-27 -0.01667429248318797 1203 28-29 0.009029976278746688 1203 30-31 -0.030277524707237546 1203 32-33 -0.07367309166411218 1203 34-35 0.02049006760164218 1203 36-37 -0.00888671396201346 1203 38-39 0.028548301888150718 1203 40-41 -0.04342384873775984 1203 42-43 0.058527428683554206 1203 44-45 0.08778115944361531 1203 46-47 -0.06040709554760326 1203 48-49 -0.08378155489769767 1203 50-51 0.01749841264999219 1203 52-53 0.05747621991111629 1203 54-55 -0.11007527886980739 1203 56-57 -0.11659887960804127 1203 58-59 -0.025822019006845665 1203 60-61 -0.04348181217490321 1203 62-63 -0.09554070758247946 1203 64-65 -0.20825796329827284 1203 66-67 0.08237606902608263 1203 68-69 -0.0690165016947688 1203 70-71 -0.200589157794127 1203 72-73 -0.2989585511980408 1203 74-75 -0.2852433396207914 1203 76-77 -0.6325179452708483 1203 78-79 -0.6006926973702065 1203 80-81 -0.6321394973596384 1203 82-83 -0.684511323889673 1203 84-85 -0.8570802650236402 1203 86-87 -0.15095504062336573 1203 88-89 -0.5685991475970624 1203 90-91 0.938936273672148 1204 1 -0.0022069003667155584 1204 2 -0.05825322089941665 1204 3 -0.3247540884278308 1204 4 -0.2860922797723404 1204 5 -0.021774949547513245 1204 6 -0.10010922971638792 1204 7 -0.2889304798155763 1204 8 -0.1412900792351124 1204 9 -0.09742492660894442 1204 10-11 -0.06205976149184522 1204 12-13 -0.0925267214629173 1204 14-15 -0.1702685369134187 1204 16-17 -0.09258331956946364 1204 18-19 -0.09993256310253429 1204 20-21 -0.18508688214859603 1204 22-23 -0.14227453283911018 1204 24-25 -0.083434192275611 1204 26-27 -0.13301625652782434 1204 28-29 -0.13409044654343205 1204 30-31 -0.07001289753740281 1204 32-33 -0.07628027156716399 1204 34-35 0.023209084982838135 1204 36-37 -0.09187869033850404 1204 38-39 -0.07409812856579379 1204 40-41 -0.12173174423863742 1204 42-43 -0.1729269012605883 1204 44-45 -0.04354451869316023 1204 46-47 -0.036772177973134035 1204 48-49 -0.1421389542467466 1204 50-51 0.03574590016712165 1204 52-53 -0.07068926696933175 1204 54-55 -0.15670652195668566 1204 56-57 -0.05434232504511982 1204 58-59 -0.09325549996191995 1204 60-61 -0.023157183588928376 1204 62-63 0.10710539919377737 1204 64-65 -0.008820195691939148 1204 66-67 -0.08934860673379319 1204 68-69 0.06890036459980564 1204 70-71 0.2779912815587764 1204 72-73 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0.021493887710846593 2401 46-47 0.048037485889949494 2401 48-49 0.07854313977826877 2401 50-51 0.01875002959729244 2401 52-53 -0.01787628478567882 2401 54-55 -0.12642649834373287 2401 56-57 0.21329116977791784 2401 58-59 0.16123948622199435 2401 60-61 0.13607249861365744 2401 62-63 0.22289437884467844 2401 64-65 0.11578884610857898 2401 66-67 0.37428782099112823 2401 68-69 0.3654552980788637 2401 70-71 0.10834193581518292 2401 72-73 0.32774607051465665 2401 74-75 0.1553314119975795 2401 76-77 0.2916257781196805 2401 78-79 0.31264951464377333 2401 80-81 0.12990788914787288 2401 82-83 0.23503228457443015 2401 84-85 0.5346709709898931 2401 86-87 0.46865228995275743 2401 88-89 0.6147341857362747 2401 90-91 0.938936273672148 2402 1 0.0036798493510872277 2402 2 0.00229080418932881 2402 3 0.17650063656611792 2402 4 0.16042134345513404 2402 5 0.15251491966276376 2402 6 0.06249507559084577 2402 7 -0.03181273537020246 2402 8 0.07009050451711119 2402 9 0.1008844922316996 2402 10-11 0.17500251782181664 2402 12-13 0.10496785550347454 2402 14-15 0.13252432025585392 2402 16-17 0.07004194233665828 2402 18-19 0.09279582581577728 2402 20-21 0.02051337853505686 2402 22-23 0.10856075199188808 2402 24-25 0.009511611083283356 2402 26-27 -0.03568477121038427 2402 28-29 0.10475889646719594 2402 30-31 0.04405849044004384 2402 32-33 0.07208841243933506 2402 34-35 0.11243572946946045 2402 36-37 0.11539676113554975 2402 38-39 -0.17519105798250934 2402 40-41 0.03789512250390459 2402 42-43 0.07399651860948353 2402 44-45 0.020471444597191635 2402 46-47 0.042400029155189145 2402 48-49 0.08185948887273753 2402 50-51 -0.03720692017625993 2402 52-53 0.050325653918598334 2402 54-55 0.11944716280276424 2402 56-57 0.09015605661497261 2402 58-59 0.047025252603738465 2402 60-61 0.006042212944848302 2402 62-63 0.05236661978852908 2402 64-65 -0.08088042634513215 2402 66-67 -0.07783117262466277 2402 68-69 -0.03991482070670571 2402 70-71 0.11531265093420728 2402 72-73 0.05400138579527436 2402 74-75 0.1733685163779981 2402 76-77 -0.04382486575151745 2402 78-79 0.14744550266134127 2402 80-81 -0.3307855495179197 2402 82-83 -0.044439302827164795 2402 84-85 0.5346709709898931 2402 86-87 -0.014196168579594826 2402 88-89 -0.7861874732499032 2402 90-91 0.938936273672148 2403 1 -0.11495533664631807 2403 2 -0.008774945155316516 2403 3 0.10410470229506075 2403 4 -0.025222889962179806 2403 5 0.015158867479449611 2403 6 0.03868184820174747 2403 7 0.09310948945869058 2403 8 0.012199002155803385 2403 9 0.07244045465481719 2403 10-11 0.00613030880295895 2403 12-13 0.10253005014030236 2403 14-15 0.03670212194701605 2403 16-17 0.06426235462156171 2403 18-19 0.03853616894453182 2403 20-21 0.04529560805595878 2403 22-23 6.64277119639678E-4 2403 24-25 0.042615146411051796 2403 26-27 0.06945769760304188 2403 28-29 0.06289354186341711 2403 30-31 0.053881686083720126 2403 32-33 0.0419283881591781 2403 34-35 0.0430340252204644 2403 36-37 0.06595334853615498 2403 38-39 0.05786628278099215 2403 40-41 -0.05764556945188559 2403 42-43 0.04417249502296983 2403 44-45 0.040467750017974424 2403 46-47 0.07263985396316741 2403 48-49 0.09061761950655978 2403 50-51 0.0926336620453867 2403 52-53 0.03568927290854873 2403 54-55 0.09010620735666919 2403 56-57 -0.002414481161615356 2403 58-59 0.11419990122950452 2403 60-61 0.155856155057009 2403 62-63 0.21395438908316322 2403 64-65 0.08324528415158738 2403 66-67 -0.1120052471160875 2403 68-69 0.0642509942993783 2403 70-71 0.048881593572495774 2403 72-73 -0.07131515048788373 2403 74-75 0.11074218798616187 2403 76-77 -0.1460972034202399 2403 78-79 0.17553022367832938 2403 80-81 0.39422336533834823 2403 82-83 0.2738347760351516 2403 84-85 0.26787384117906043 2403 86-87 -0.14613494408979477 2403 88-89 -0.15181633691181418 2403 90-91 0.938936273672148 2404 1 -0.03425435020392342 2404 2 -0.1163112789574754 2404 3 -0.013571130344601556 2404 4 0.012810396488823983 2404 5 0.1035918496737338 2404 6 -0.020984282666915988 2404 7 0.08662489843521826 2404 8 0.03964788606943159 2404 9 0.08346138904826006 2404 10-11 0.037887702337023654 2404 12-13 0.026829446410765456 2404 14-15 0.05234491211512449 2404 16-17 -0.025427600122640115 2404 18-19 0.021846898299564543 2404 20-21 0.02122416513280001 2404 22-23 0.039999385283863376 2404 24-25 -0.012996022106982252 2404 26-27 0.09247608217205538 2404 28-29 0.0268641160283849 2404 30-31 0.0016521444780508432 2404 32-33 -0.019743740011044508 2404 34-35 0.09699201681755198 2404 36-37 0.15006208219078587 2404 38-39 0.10475942143222738 2404 40-41 0.09608129915321229 2404 42-43 0.11902563844478209 2404 44-45 0.1413627854522801 2404 46-47 0.12479509352440488 2404 48-49 0.12462292515417062 2404 50-51 0.0522724049555805 2404 52-53 0.07314774675959512 2404 54-55 0.10064491156634858 2404 56-57 0.0777274305621205 2404 58-59 0.1579099843562375 2404 60-61 0.10856214176114065 2404 62-63 0.04883788791278931 2404 64-65 0.01935261950501399 2404 66-67 0.09501893756764446 2404 68-69 0.2224433528822729 2404 70-71 0.17927745808439965 2404 72-73 -0.10886823683602387 2404 74-75 0.20785476155372606 2404 76-77 0.12677215463911296 2404 78-79 -0.09409762369845964 2404 80-81 0.13770534889894037 2404 82-83 -0.1354118384985128 2404 84-85 -0.2763571439597996 2404 86-87 -0.08071166366651994 2404 88-89 -0.03232463779313832 2404 90-91 -1.8467780120421367 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 19.0 31 489.0 32 2974.0 33 5496.0 34 7498.0 35 9143.0 36 13247.0 37 19146.0 38 48141.0 39 265083.0 40 312873.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.500415869400932 13.215730241818191 23.83699088887882 33.44686299990206 2 32.05394175705449 14.965416514984714 25.634176758085196 27.3464649698756 3 29.902983296521462 17.54223376684125 20.181286900186958 32.373496036450334 4 27.6973406284671 19.214628078274075 18.107056039315374 34.980975253943456 5 27.276793610374956 19.861454826643126 19.3986630785445 33.46308848443742 6 27.427208237283825 22.873694104302093 22.667294246969416 27.031803411444667 7 26.29317842624494 22.382982828759744 22.094870846604852 29.22896789839046 8 25.49038238058555 23.384431428325016 22.615109580490827 28.510076610598606 9 25.596944346588046 23.450502770757293 23.10085088779712 27.851701994857546 10-11 25.944695947575607 22.248720598618057 22.531204822623295 29.27537863118304 12-13 26.224695187462814 21.558552803719873 22.410756180667114 29.805995828150195 14-15 26.515949943649332 21.343894028583165 22.383275179832456 29.75688084793505 16-17 26.79266023396856 21.2317773921992 21.920702695038365 30.05485967879388 18-19 27.061038518715584 21.4931392512012 21.72285410658243 29.722968123500788 20-21 27.044009068730272 21.623016215252246 21.850465349819984 29.48250936619749 22-23 26.97457568896185 21.221545104654375 21.883427933267942 29.920451273115834 24-25 27.08106456719616 21.492189110214895 21.805589460159126 29.621156862429814 26-27 27.25961798485329 21.693253560470627 21.850976964197226 29.196151490478854 28-29 27.51169769729678 21.37751440194472 21.632590712883474 29.478197187875033 30-31 27.49226341094042 21.592575213386603 21.756077808816716 29.159083566856264 32-33 27.43266984691457 21.644558031944968 21.861172629252522 29.06159949188794 34-35 27.4529888812242 21.331580236203962 21.911046592584174 29.304384289987663 36-37 27.362444820576503 21.59605685075401 22.021501900978983 29.019996427690504 38-39 27.691307329360882 21.535285316051993 21.983255969830513 28.790151384756612 40-41 27.4998969397991 21.44839824817289 21.974986798479026 29.07671801354898 42-43 27.46572676325205 21.57739539397559 22.175357396140495 28.781520446631863 44-45 27.46983654859253 21.799503958078866 22.19053324506522 28.540126248263388 46-47 27.547874118833114 21.510143587129036 22.229168081102582 28.712814212935267 48-49 27.553787767383458 21.848099046412454 22.16313360406321 28.43497958214088 50-51 27.589535306432 21.781492484925977 22.321932232710314 28.30703997593171 52-53 27.637719962718478 21.716136824577877 22.285133819017066 28.361009393686576 54-55 27.698981656523635 21.937296316642456 22.38185632149078 27.98186570534313 56-57 27.779988348703515 21.89080756674764 22.403904283500225 27.925299801048613 58-59 27.79968884088274 21.87478322911815 22.24738140774727 28.07814652225184 60-61 27.93095033057782 22.110704998920045 22.22094435577298 27.737400314729154 62-63 27.845233087307353 22.13692046859663 22.2984945249659 27.719351919130116 64-65 27.96886083777442 22.01458738791175 22.221211735389875 27.795340038923953 66-67 27.888669287949757 22.302077109178807 22.404449217166757 27.404804385704672 68-69 28.254902433729708 22.205070069225016 21.98605851564196 27.553968981403315 70-71 28.253679973990742 22.017623515958697 21.987354118319715 27.74134239173085 72-73 28.181758487096985 22.24046227592094 22.296276840239017 27.281502396743058 74-75 28.018258601415972 22.269749099490745 22.189013787107193 27.52297851198609 76-77 28.196742634693823 22.27569611550361 21.879906720057978 27.647654529744596 78-79 28.494812631101535 22.148647882533023 21.65655649413232 27.699982992233117 80-81 28.484489899416786 22.059279294508777 21.712732088017354 27.74349871805708 82-83 28.92749107902983 21.703769449526327 21.102396435234663 28.266343036209175 84-85 29.1232164126498 21.40790976931865 20.749167456671287 28.719706361360263 86-87 29.684827754703154 20.756692611078147 19.95043803008621 29.60804160413249 88-89 29.191469687337456 20.493556030746113 20.29705831358724 30.01791596832919 90-91 25.64211120519334 20.60400790290714 22.071690657634775 31.682190234264745 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.5 9 2.0 10 1.5 11 1.5 12 3.0 13 5.0 14 6.0 15 11.0 16 20.0 17 28.0 18 38.0 19 63.0 20 95.0 21 155.0 22 245.0 23 332.0 24 454.5 25 609.0 26 813.5 27 1094.0 28 1443.0 29 1851.5 30 2336.0 31 2928.5 32 3581.0 33 4259.0 34 5185.0 35 6334.5 36 7576.5 37 8857.0 38 10386.5 39 12320.0 40 14291.0 41 16380.0 42 18702.5 43 20940.5 44 23100.5 45 25528.0 46 28139.0 47 30776.0 48 33254.0 49 35616.5 50 38187.5 51 40823.0 52 43116.5 53 45525.0 54 48071.0 55 50077.0 56 51930.5 57 53646.0 58 54898.0 59 55595.5 60 55882.5 61 55915.0 62 55310.5 63 54052.5 64 52009.0 65 49129.5 66 45626.0 67 41910.0 68 37809.5 69 33366.0 70 29146.5 71 25125.5 72 21171.5 73 17117.0 74 13475.5 75 10905.5 76 8748.0 77 6647.5 78 5111.5 79 3866.0 80 2797.5 81 2021.0 82 1484.0 83 1059.5 84 753.0 85 561.5 86 387.5 87 258.0 88 180.0 89 123.5 90 83.5 91 62.0 92 42.5 93 27.5 94 20.5 95 15.5 96 10.0 97 6.5 98 4.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.016371660071713718 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.06416277139487135 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 3.186854861069062E-4 64-65 0.0 66-67 0.0 68-69 4.8240197591849335E-4 70-71 0.0011210762331838565 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0024307243558580457 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 15991.0 32-33 34336.0 34-35 36485.0 36-37 38104.0 38-39 39785.0 40-41 40501.0 42-43 40044.0 44-45 39130.0 46-47 37246.0 48-49 34975.0 50-51 33091.0 52-53 31275.0 54-55 28955.0 56-57 26227.0 58-59 24192.0 60-61 21744.0 62-63 19992.0 64-65 18162.0 66-67 16374.0 68-69 14877.0 70-71 13443.0 72-73 12005.0 74-75 10857.0 76-77 9949.0 78-79 8816.0 80-81 8114.0 82-83 7193.0 84-85 6441.0 86-87 5808.0 88-89 5250.0 90-91 4747.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.93506383046629 #Duplication Level Percentage of deduplicated Percentage of total 1 99.93975691490778 99.87485986492592 2 0.056132152818156984 0.11219140549648013 3 0.003486585646102339 0.010452964772808744 4 6.243466279794359E-4 0.002495764804778453 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0014617553635458676 0.0 0.0 0.0 0.0 2 0.0014617553635458676 0.0 0.0 0.0 0.0 3 0.0014617553635458676 0.0 0.0 0.0 1.4617553635458676E-4 4 0.0014617553635458676 0.0 0.0 0.0 1.4617553635458676E-4 5 0.0014617553635458676 0.0 0.0 0.0 1.4617553635458676E-4 6 0.0021926330453188015 0.0 0.0 0.0 2.9235107270917353E-4 7 0.0026311596543825617 0.0 0.0 0.0 2.9235107270917353E-4 8 0.008916707717629794 0.0 0.0 0.0 2.9235107270917353E-4 9 0.00906288325398438 0.0 0.0 0.0 2.9235107270917353E-4 10-11 0.009209058790338967 0.0 1.4617553635458676E-4 0.0 2.9235107270917353E-4 12-13 0.009209058790338967 0.0 1.4617553635458676E-4 0.0 4.385266090637603E-4 14-15 0.009209058790338967 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 16-17 0.009209058790338967 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 18-19 0.009209058790338967 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 20-21 0.009209058790338967 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 22-23 0.009355234326693555 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 24-25 0.009574497631225434 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 26-27 0.00972067316758002 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 28-29 0.010086112008466487 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 30-31 0.010086112008466487 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 32-33 0.010451550849352953 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 34-35 0.0110362529947713 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 36-37 0.011255516299303181 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 38-39 0.011547867372012355 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 40-41 0.011913306212898822 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 42-43 0.012132569517430702 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 44-45 0.01227874505378529 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 46-47 0.012498008358317169 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 48-49 0.012717271662849049 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 50-51 0.012936534967380928 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 52-53 0.013082710503735516 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 54-55 0.013228886040090104 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 56-57 0.01337506157644469 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 58-59 0.013448149344621983 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 60-61 0.013448149344621983 0.0 1.4617553635458676E-4 0.0 5.847021454183471E-4 62-63 0.013521237112799275 0.0 1.4617553635458676E-4 0.0 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