##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-31B_S33_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1317985
Sequences flagged as poor quality	0
Sequence length	61
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.40548337044807	40.0	40.0	40.0	40.0	40.0
2	39.12229350106413	40.0	40.0	40.0	40.0	40.0
3	39.080094234759876	40.0	40.0	40.0	40.0	40.0
4	38.98458783673562	40.0	40.0	40.0	40.0	40.0
5	39.012195131204074	40.0	40.0	40.0	40.0	40.0
6	39.12761070877134	40.0	40.0	40.0	40.0	40.0
7	39.13359560237787	40.0	40.0	40.0	40.0	40.0
8	39.14966862293577	40.0	40.0	40.0	40.0	40.0
9	39.16761116401173	40.0	40.0	40.0	40.0	40.0
10	39.174284988068905	40.0	40.0	40.0	40.0	40.0
11	39.16986612138985	40.0	40.0	40.0	40.0	40.0
12	39.16742299798556	40.0	40.0	40.0	40.0	40.0
13	38.99186409557013	40.0	40.0	40.0	40.0	40.0
14	39.16504967810711	40.0	40.0	40.0	40.0	40.0
15	39.06784371597552	40.0	40.0	40.0	40.0	40.0
16	39.26260465786788	40.0	40.0	40.0	40.0	40.0
17	39.2061730596327	40.0	40.0	40.0	40.0	40.0
18	39.27931804990193	40.0	40.0	40.0	40.0	40.0
19	39.234719666764036	40.0	40.0	40.0	40.0	40.0
20	39.24784576455726	40.0	40.0	40.0	40.0	40.0
21	39.21746909107463	40.0	40.0	40.0	40.0	40.0
22	39.28275663228337	40.0	40.0	40.0	40.0	40.0
23	39.27820726336036	40.0	40.0	40.0	40.0	40.0
24	39.27672924957416	40.0	40.0	40.0	40.0	40.0
25	39.23635246228144	40.0	40.0	40.0	40.0	40.0
26	39.19426397113776	40.0	40.0	40.0	40.0	40.0
27	39.219435729541686	40.0	40.0	40.0	40.0	40.0
28	39.23220977476982	40.0	40.0	40.0	40.0	40.0
29	39.233411609388575	40.0	40.0	40.0	40.0	40.0
30	39.22631289430456	40.0	40.0	40.0	40.0	40.0
31	39.16892529125901	40.0	40.0	40.0	40.0	40.0
32	39.20535362693809	40.0	40.0	40.0	40.0	40.0
33	39.20216390930094	40.0	40.0	40.0	40.0	40.0
34	39.2053081028995	40.0	40.0	40.0	40.0	40.0
35	39.20334146443245	40.0	40.0	40.0	40.0	40.0
36	39.17281911402634	40.0	40.0	40.0	40.0	40.0
37	39.173732629734026	40.0	40.0	40.0	40.0	40.0
38	39.185908792588684	40.0	40.0	40.0	40.0	40.0
39	37.95669146462213	40.0	40.0	40.0	40.0	40.0
40	39.13229968474603	40.0	40.0	40.0	40.0	40.0
41	39.206082011555516	40.0	40.0	40.0	40.0	40.0
42	39.165890355353056	40.0	40.0	40.0	40.0	40.0
43	39.18933827016241	40.0	40.0	40.0	40.0	40.0
44	39.159632317515	40.0	40.0	40.0	40.0	40.0
45	38.997147918982385	40.0	40.0	40.0	40.0	40.0
46	39.02762474534991	40.0	40.0	40.0	40.0	40.0
47	39.0445080937947	40.0	40.0	40.0	40.0	40.0
48	39.21339620708885	40.0	40.0	40.0	40.0	40.0
49	39.195031809921964	40.0	40.0	40.0	40.0	40.0
50	39.18888606471242	40.0	40.0	40.0	40.0	40.0
51	39.14553200529596	40.0	40.0	40.0	40.0	40.0
52	39.160093627772696	40.0	40.0	40.0	40.0	40.0
53	39.07049928489323	40.0	40.0	40.0	40.0	40.0
54	38.95366639225788	40.0	40.0	40.0	40.0	40.0
55	39.01308740236042	40.0	40.0	40.0	40.0	40.0
56	39.015730831534505	40.0	40.0	40.0	40.0	40.0
57	38.89128631964704	40.0	40.0	40.0	40.0	40.0
58	38.840663588735836	40.0	40.0	40.0	40.0	40.0
59	38.77458392925564	40.0	40.0	40.0	40.0	40.0
60	38.6894448722861	40.0	40.0	40.0	40.0	40.0
61	38.55730983281297	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.03940497651879582
1101	2	0.04152992140194556
1101	3	0.09038381178820032
1101	4	-0.0298789434229505
1101	5	0.0365175401957174
1101	6	0.09150002842468297
1101	7	-0.07641597529125477
1101	8	0.05044884359580948
1101	9	-0.05161626844699896
1101	10	0.04621651595503806
1101	11	-0.04665549490027843
1101	12	-0.02546832905436247
1101	13	-0.03940862611381846
1101	14	0.061885007229939504
1101	15	0.10426685905566302
1101	16	0.038640102422640155
1101	17	0.05073138633467522
1101	18	0.032307693859479514
1101	19	0.05076701989218435
1101	20	-0.02013493582617798
1101	21	0.0237325173533236
1101	22	-0.06143784963673227
1101	23	0.10843507018539356
1101	24	0.0510946014795195
1101	25	0.08019501777524596
1101	26	0.03883601037421869
1101	27	-0.0020163340592134205
1101	28	0.004231311678509542
1101	29	0.03144126423310212
1101	30	2.8121961587146416E-4
1101	31	0.011034664246544423
1101	32	0.11590563551526856
1101	33	-0.008591177303983955
1101	34	0.026174960926141466
1101	35	-0.1180284148280819
1101	36	-0.06812816493052765
1101	37	-0.16600594473471375
1101	38	-0.10661975705741611
1101	39	1.2496508269676383
1101	40	-0.08490526110740859
1101	41	-0.07324192957690201
1101	42	0.03477807126535737
1101	43	-0.10434578257414984
1101	44	-0.055292566890443595
1101	45	-0.09553961029186553
1101	46	0.0653751202474453
1101	47	-0.02547855400995047
1101	48	0.029896192002411226
1101	49	0.035035825634999185
1101	50	-0.005058965743423016
1101	51	0.025316489896162864
1101	52	-0.051521704015726755
1101	53	-0.025396592717136457
1101	54	-0.06612476973502623
1101	55	-0.01818225123704309
1101	56	0.012799103637838982
1101	57	0.037927363454720364
1101	58	-0.010223588461975908
1101	59	0.01871999990812867
1101	60	-0.05337700263231682
1101	61	0.014171394980678542
1102	1	0.08171297553460732
1102	2	-0.07563053397100106
1102	3	0.06157687682235746
1102	4	-0.08933079013523582
1102	5	-0.11708097524613237
1102	6	-0.08424144980392612
1102	7	0.019506419831039068
1102	8	-0.1033418755983675
1102	9	-0.12942062002174026
1102	10	-0.10184989384926268
1102	11	0.009301242443299884
1102	12	-0.10980261000217695
1102	13	0.02528588465151671
1102	14	-0.12623793683172835
1102	15	0.005776294646842928
1102	16	-0.10422135706723878
1102	17	-0.11434437397770836
1102	18	-0.11239219679340096
1102	19	-0.02912703630900637
1102	20	-0.05256420723083011
1102	21	0.004762961200860616
1102	22	0.060074453107354486
1102	23	-0.11907646601559208
1102	24	-0.018029228855006352
1102	25	-0.1094846403656149
1102	26	-0.01932993416522777
1102	27	-0.05155037627451975
1102	28	-0.07966676217181146
1102	29	-0.03701326083619705
1102	30	-0.10661859974440802
1102	31	-0.16681787458099961
1102	32	-0.09180255284168481
1102	33	0.0038048287656593516
1102	34	-0.12939511971991635
1102	35	0.01329536693296518
1102	36	-0.11820292219377393
1102	37	-0.026450117155562225
1102	38	-0.08615528283123552
1102	39	1.1493036561000949
1102	40	-0.06552812394821927
1102	41	-0.06968229790835778
1102	42	-0.04836436466502647
1102	43	-0.11059626677538859
1102	44	-0.17333066185590695
1102	45	-0.031946068065316524
1102	46	-0.033560738906736276
1102	47	-0.061755989902842146
1102	48	-0.12179709806462569
1102	49	-0.11966047766888721
1102	50	-0.09690600518086256
1102	51	-0.04364239962961847
1102	52	-0.0568120782200765
1102	53	-0.06819487033524041
1102	54	-0.10683788655060766
1102	55	-0.024950731366558898
1102	56	-0.1284861033299336
1102	57	-0.0168602479910831
1102	58	-0.12189396856874879
1102	59	-0.17623912725964175
1102	60	-0.0035417048903028103
1102	61	-0.09874399288354141
1103	1	0.08078459474352684
1103	2	0.020633809432929695
1103	3	-0.016313320060973524
1103	4	-0.03449742180072235
1103	5	0.041111668072375096
1103	6	0.03682774220347795
1103	7	0.021042846996756737
1103	8	-0.015587009811468988
1103	9	0.03340350618911714
1103	10	-0.00558917516291757
1103	11	0.01087391678485261
1103	12	-0.0031321555056322836
1103	13	-0.005100122851189326
1103	14	-0.0402274663819
1103	15	0.08002999361450236
1103	16	0.02925433704918845
1103	17	0.004501959971605629
1103	18	0.11391704553415138
1103	19	0.04807899513043168
1103	20	-0.00841152234588094
1103	21	0.08282306672091977
1103	22	0.08868298972504363
1103	23	0.0020856093798897746
1103	24	0.01600958779251016
1103	25	-0.04393977848208408
1103	26	-6.875585434471532E-4
1103	27	0.004908677896203528
1103	28	0.07277526670042533
1103	29	0.06670214265037089
1103	30	0.06981407554410168
1103	31	0.06269289955219648
1103	32	0.05428398683753244
1103	33	-0.03759397693520583
1103	34	0.017907965798272585
1103	35	0.04768215646849683
1103	36	0.11835067820421585
1103	37	0.050184568174771016
1103	38	-0.011076507523625878
1103	39	1.2471927791608408
1103	40	0.07235703910856728
1103	41	0.03160862260453001
1103	42	0.0550769195542955
1103	43	0.03764063661025574
1103	44	0.09427226936258393
1103	45	0.10481090164359586
1103	46	0.04709330513403387
1103	47	0.0560563933557674
1103	48	0.14961948914142909
1103	49	0.07897495208782601
1103	50	0.09590903431610087
1103	51	0.1386396636593119
1103	52	0.1151182447399961
1103	53	0.10535071739798951
1103	54	-0.003190899608419784
1103	55	0.05583380250722314
1103	56	0.1587573601148975
1103	57	0.13579473303918377
1103	58	0.14294647476139488
1103	59	0.14827413546872492
1103	60	0.3156210215950779
1103	61	0.269552686625957
1104	1	0.006942871778996107
1104	2	0.01753421861404547
1104	3	-0.04423733044112055
1104	4	-0.12335956329444286
1104	5	0.0036801745005305975
1104	6	-0.003301455820455601
1104	7	0.08159462684678687
1104	8	0.06011892554359122
1104	9	-0.09594041262848663
1104	10	0.02455027602916715
1104	11	-0.04254117171795713
1104	12	0.017529531822717104
1104	13	-0.048345797675814595
1104	14	0.03449773115031718
1104	15	-0.02277861796603986
1104	16	-0.07337190592792808
1104	17	0.009065227692765632
1104	18	0.012391768570275019
1104	19	-0.10015155660889263
1104	20	0.0977958996510182
1104	21	0.04667220600865818
1104	22	-0.016659870003785215
1104	23	0.019097222882734854
1104	24	-0.046719808270218266
1104	25	0.028960382137377394
1104	26	-0.024638635126102315
1104	27	0.06650333598209102
1104	28	-0.028874291089479698
1104	29	-0.015005746075061666
1104	30	-0.029955059312754884
1104	31	-0.08845554984186776
1104	32	-0.03413236473671333
1104	33	0.02484498024059434
1104	34	-0.0363689832155174
1104	35	-0.05594778926671751
1104	36	-0.15424310316149104
1104	37	-0.09526857727837523
1104	38	-0.10816279759237091
1104	39	1.1881161586807139
1104	40	-0.15003674468872674
1104	41	-0.058728678259086564
1104	42	-0.042902301231947604
1104	43	-0.04322295302526413
1104	44	-0.08724122794038891
1104	45	-0.17565850163282448
1104	46	-0.12231632041594054
1104	47	-0.0630005853503377
1104	48	-0.04500968794388882
1104	49	-0.014188386689546917
1104	50	-0.07321150322548675
1104	51	-0.069413193516354
1104	52	0.008650103184137947
1104	53	-0.05442174062007865
1104	54	0.05323124453301631
1104	55	-0.03192872385179868
1104	56	0.06356500176464408
1104	57	0.022353356439914762
1104	58	-0.03324456107876017
1104	59	-0.12296336383895579
1104	60	-0.20432267378721036
1104	61	0.07319303412594991
1201	1	-0.055121531459995765
1201	2	0.0614849544354783
1201	3	-0.052960321561300816
1201	4	-0.03029441646069131
1201	5	-0.03883694162836804
1201	6	0.003332084178424566
1201	7	-0.01519122926056582
1201	8	-0.023371583935080764
1201	9	-0.10235511419441679
1201	10	-0.05162601474145845
1201	11	-0.056144259892974446
1201	12	0.03164962579558761
1201	13	-0.20032498614084204
1201	14	-0.10109273825356269
1201	15	0.03019988708479815
1201	16	0.04919311758124678
1201	17	-0.045767477163941805
1201	18	0.03820604130405769
1201	19	0.011191158510428068
1201	20	-0.025775324736336813
1201	21	-0.09120102179895895
1201	22	-0.0015960352888058082
1201	23	0.029528065606740483
1201	24	0.013959530053540448
1201	25	-0.019660502617291797
1201	26	0.0016383897106919676
1201	27	-0.03242896042179666
1201	28	0.04878371823759409
1201	29	0.08686582403142751
1201	30	0.06101926865949281
1201	31	0.015599845460130268
1201	32	-0.011538664274553412
1201	33	-0.06241274167867772
1201	34	-0.0041737465075613045
1201	35	-0.020226518307808306
1201	36	-0.04969942460597565
1201	37	0.07219890127888107
1201	38	0.056445192107631215
1201	39	1.2016175781670881
1201	40	0.06829334482075211
1201	41	0.06257209738637926
1201	42	-0.005564817263000066
1201	43	0.052080272698830754
1201	44	0.04643212560814902
1201	45	0.023616687240703982
1201	46	0.04706234712243429
1201	47	-0.1012622057851047
1201	48	0.036142897609401814
1201	49	0.011243830613366868
1201	50	-0.038890798237474655
1201	51	0.007715956264419788
1201	52	0.005057505576495203
1201	53	0.08062664774884354
1201	54	-0.012560250767194248
1201	55	0.06519131677920598
1201	56	0.03132052640927441
1201	57	0.1073748231224485
1201	58	0.11197890259565213
1201	59	-0.021653934957114984
1201	60	-0.08572911192273125
1201	61	-0.010151127150678008
1202	1	0.0058323240875566285
1202	2	0.057399554524174334
1202	3	0.06668506902070703
1202	4	0.015536962306867963
1202	5	0.08118432038685341
1202	6	0.05551141214180433
1202	7	0.07028143135441667
1202	8	0.031603101936035216
1202	9	0.08182188807877111
1202	10	0.08550345992730968
1202	11	0.04769897403803469
1202	12	-0.0025139533148816895
1202	13	0.029799153235394726
1202	14	0.00971269611986969
1202	15	0.0062619326375354945
1202	16	-0.020999671520911534
1202	17	-0.07096344561682599
1202	18	-0.0015667841064086474
1202	19	-0.004152311455484892
1202	20	-0.041837823256472007
1202	21	0.015409202384446985
1202	22	0.09988993799229462
1202	23	0.02514617794288654
1202	24	0.0115405011218499
1202	25	-0.03472029602983895
1202	26	0.03526984651218612
1202	27	0.052568541966287796
1202	28	0.008320231928244937
1202	29	-0.0682842036899487
1202	30	0.035790585894410754
1202	31	0.07693391909775471
1202	32	-0.03135667432700018
1202	33	-0.03605118287143938
1202	34	0.002623052202537224
1202	35	-0.020869823523987918
1202	36	-0.25967589432218574
1202	37	-0.19291056412693308
1202	38	-0.19054900888017556
1202	39	1.1002050405098842
1202	40	-0.1450671116514215
1202	41	-0.23328355974749115
1202	42	-0.04604523265697935
1202	43	-0.09580969419909025
1202	44	-0.018158509107848886
1202	45	-0.09882978053438762
1202	46	-0.04140721901600841
1202	47	-0.10004033766290377
1202	48	-0.1575578586288202
1202	49	-0.02476913660792235
1202	50	-0.03541690431580946
1202	51	0.03591981126864141
1202	52	-0.23168979500660924
1202	53	-0.0026706254903601234
1202	54	0.05441578348263221
1202	55	-0.07849909327182303
1202	56	-0.14367908181967692
1202	57	-0.0701506502885394
1202	58	-0.002254442645231336
1202	59	-0.02389246324315053
1202	60	-0.08014022627857997
1202	61	-0.07277135367784382
1203	1	0.02101375037413078
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2403	2	0.0739975446852128
2403	3	0.08634196680483797
2403	4	0.19574551463416157
2403	5	0.20919662609541234
2403	6	0.07909338245673325
2403	7	0.19702672561221846
2403	8	0.12316009642789538
2403	9	0.13185412398957652
2403	10	0.15612393113223533
2403	11	0.17885428105004308
2403	12	0.08844177136241171
2403	13	0.17395183932770664
2403	14	0.19091916012076382
2403	15	0.15167532315457066
2403	16	0.12581808345813528
2403	17	0.141284098146933
2403	18	0.17191637613409938
2403	19	0.10788710044937488
2403	20	-0.023864901421546847
2403	21	0.16759003182844623
2403	22	0.16486071543837255
2403	23	0.08934469509098619
2403	24	0.07067307480893703
2403	25	0.195833741167597
2403	26	0.05806624169641594
2403	27	0.09290968199628225
2403	28	0.04350993325552821
2403	29	0.08936551810012361
2403	30	0.1332369411341503
2403	31	0.13753182516511941
2403	32	0.037268674311384586
2403	33	0.09790514447723098
2403	34	0.13601244798944379
2403	35	0.12727661160474213
2403	36	0.09389107928641494
2403	37	0.10241032334170797
2403	38	0.12879323838327394
2403	39	1.3121176111135355
2403	40	0.12220502951383594
2403	41	0.057217358275181596
2403	42	0.08943108796418642
2403	43	0.053880560744914874
2403	44	0.026551297616848046
2403	45	0.2747241585537594
2403	46	0.061866127491995826
2403	47	0.07327113131353968
2403	48	0.08322337166172389
2403	49	0.14532504831408488
2403	50	0.09499697674769436
2403	51	0.19370618528930095
2403	52	0.0449693032288252
2403	53	0.13718487353555275
2403	54	0.1561109719225513
2403	55	0.11120573791011168
2403	56	0.1239890004239399
2403	57	0.12066071357079977
2403	58	0.19670069337629315
2403	59	0.08543002200541139
2403	60	-0.013316584741907889
2403	61	0.1640287416283286
2404	1	0.014035964417807634
2404	2	-0.009943111223165602
2404	3	0.030786411249287937
2404	4	0.11663741121580529
2404	5	0.11349704832920082
2404	6	0.016767626195530738
2404	7	0.06039939031231256
2404	8	0.15153073671941542
2404	9	0.07078928507466031
2404	10	0.07348757275407536
2404	11	0.1159356622750991
2404	12	0.04398575886891365
2404	13	0.09967628839936538
2404	14	-0.02337316447869142
2404	15	0.09828299277108954
2404	16	0.08959273786267374
2404	17	0.0518254828424034
2404	18	0.01789647908973535
2404	19	-0.0022349424680854213
2404	20	0.10457803917651631
2404	21	0.04971450403620992
2404	22	0.03248753017606276
2404	23	0.025517984465736276
2404	24	0.06876145470366879
2404	25	-0.045811235407100526
2404	26	0.06173909726932436
2404	27	0.09190904575358161
2404	28	0.13381301614059993
2404	29	0.03429292859234323
2404	30	0.01630131732710538
2404	31	0.04697713721323993
2404	32	0.01636810169295444
2404	33	0.029963100402959242
2404	34	0.07706167003814102
2404	35	0.2265825286618366
2404	36	0.06620584590102396
2404	37	0.15435953960638216
2404	38	0.1366161315523371
2404	39	1.3895801595547397
2404	40	0.17706071810195567
2404	41	0.04524731952221828
2404	42	0.1845725073638107
2404	43	0.050684887195259876
2404	44	0.18538061105313375
2404	45	0.1359249220449925
2404	46	0.1024439515419715
2404	47	0.16797874887383557
2404	48	0.17064890234597385
2404	49	0.12270662041194669
2404	50	0.10269551338949157
2404	51	0.10619967826171006
2404	52	0.1310731843092796
2404	53	0.07584240144347376
2404	54	0.054147295596564504
2404	55	0.08400636258476624
2404	56	0.1460348098981683
2404	57	0.02434513719275344
2404	58	0.1210890327828551
2404	59	0.012259219761212137
2404	60	0.14893687121274723
2404	61	0.09042992156933138
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	6.0
17	54.0
18	161.0
19	260.0
20	309.0
21	408.0
22	591.0
23	850.0
24	1212.0
25	1651.0
26	2058.0
27	2549.0
28	3548.0
29	4279.0
30	5202.0
31	6615.0
32	8732.0
33	10578.0
34	13462.0
35	17273.0
36	25320.0
37	38373.0
38	95470.0
39	540287.0
40	538737.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	29.174914737269393	15.141674601759503	23.759299233299316	31.924111427671786
2	32.797672672957574	15.295056099784224	25.635586305929962	26.27168492132824
3	28.97384031041395	19.45110024264368	20.3367724293655	31.238287017576873
4	26.814968922232747	19.491056037099085	19.952973632456843	33.74100140821133
5	26.44749371199217	19.8502259130415	19.656293508651466	34.04598686631486
6	28.268758749151168	22.770517115141676	22.94024590568178	26.02047823002538
7	27.122311710679558	22.264593299620252	22.405490199053858	28.20760479064633
8	24.67713972465544	25.323656946019874	22.929396009818017	27.069807319506673
9	24.696563314453503	25.31333816393965	23.497308391218414	26.492790130388432
10	26.452577229634635	22.824463100869888	23.00026176322189	27.72269790627359
11	25.059010535021265	24.160441886667904	23.06695447975508	27.71359309855575
12	26.78467509114292	22.823476746700454	22.994116018012345	27.397732144144282
13	25.36470445414781	21.4779379127987	22.693657363323556	30.463700269729927
14	25.35871045573356	23.276440930663096	22.807391586398936	28.557457027204407
15	25.44831693835666	22.380983091613334	22.731745808943195	29.438954161086812
16	25.756287059412664	23.369689336373327	22.258295807615415	28.615727796598595
17	25.840885897790944	22.009658683520676	22.34152892483602	29.80792649385236
18	27.392421006308876	22.606402956027573	22.450255503666583	27.550920533996976
19	25.87624290109523	21.986593170635476	23.624775699268202	28.51238822900109
20	25.8493837183276	22.289555647446672	22.264517426222604	29.596543208003123
21	26.052572677230774	22.685235416184554	23.622423623941092	27.63976828264358
22	27.755323467262528	21.915803290629256	21.97301183245636	28.355861409651855
23	26.012587396669918	23.814459193389908	22.058293531413483	28.114659878526687
24	26.086791579570328	24.61727561391063	21.971039124117496	27.324893682401548
25	26.278371908633254	22.38690121662993	21.773616543435622	29.561110331301194
26	26.25955530601638	22.908834319055224	23.331069776970146	27.500540597958246
27	26.206443927662303	23.71658251042311	21.680140517532447	28.39683304438214
28	26.357280242187887	23.21460411157942	21.503658994601608	28.924456651631086
29	26.549619305227296	25.07797888443343	21.5848435300857	26.78755828025357
30	28.160563284104146	24.176223553378833	21.448499034511016	26.214714128006012
31	26.645371533059937	24.082292287089764	22.63348975898815	26.63884642086215
32	26.874129826970716	24.39026240814577	20.990375459508265	27.74523230537525
33	27.07959498780335	26.455915659131175	20.946065395281433	25.51842395778404
34	27.200157816667108	24.981088555636067	20.555696764378958	27.26305686331787
35	27.444773650686464	25.507270568329687	21.743798298159692	25.30415748282416
36	27.822319677386314	27.742576736457547	19.769117250955055	24.665986335201083
37	27.933094837953394	26.143013767228002	19.459629661946074	26.46426173287253
38	28.01928701768229	26.750456188803362	20.62656251778283	24.603694275731513
39	29.82814538267955	27.137651257589308	18.755079765034928	24.279123594696216
40	28.08173082394716	27.194467311843457	18.472440885139058	26.25136097907032
41	28.188332947643563	27.607597962040536	19.70508010333957	24.49898898697633
42	28.229532202566798	28.14462987059792	17.902859288990392	25.72297863784489
43	28.049181136355877	29.670368023915294	17.732523511269097	24.547927328459732
44	28.188181200848266	28.41504265981783	18.8964214311999	24.500354708134008
45	28.1199710163621	28.730448373843405	17.275006923447535	25.874573686346963
46	28.045387466473443	28.580370793294307	18.58754082937211	24.786700910860137
47	29.348209577498984	28.88811329415737	17.009146538086547	24.754530590257097
48	29.26839076317257	28.964973045975484	17.01673387785142	24.749902313000526
49	29.063001475737583	28.9355341676878	16.735850559755992	25.26561379681863
50	28.849417861356542	29.126962749955425	16.799053099997344	25.224566288690692
51	28.672860465028055	29.232654392880043	16.77613933390744	25.31834580818446
52	28.32847111310068	29.150862870214763	16.85239209854437	25.668273918140187
53	27.979681104109684	29.256706259934674	16.93463886159554	25.828973774360104
54	27.6243659829209	29.238724264691935	17.018554839394987	26.11835491299218
55	27.19158412273281	29.14631046635584	17.02174152209623	26.640363888815124
56	26.896133112288833	29.016794576569538	17.14564278045653	26.941429530685102
57	26.436112702344865	29.02559589069678	17.33585738836178	27.202434018596573
58	25.840354784007406	28.908599111522516	17.49898519330645	27.752060911163635
59	25.401578925405072	28.785532460536352	17.67546671623729	28.137421897821298
60	25.128358820472158	28.708445088525288	17.94314806314184	28.220048027860713
61	25.0628042049037	28.225055672105526	18.145047174284986	28.567092948705792
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	5.5
2	3.0
3	3.0
4	5.0
5	7.0
6	9.5
7	10.0
8	8.0
9	9.5
10	11.0
11	11.5
12	12.0
13	12.0
14	16.0
15	20.0
16	30.5
17	57.5
18	74.0
19	94.5
20	156.5
21	198.0
22	246.0
23	294.0
24	442.5
25	766.5
26	942.0
27	1181.5
28	1421.0
29	1880.5
30	3013.0
31	3686.0
32	4594.0
33	5502.0
34	6409.5
35	8891.0
36	10465.0
37	12435.5
38	14406.0
39	17145.5
40	23185.0
41	26485.0
42	30554.0
43	39634.5
44	44646.0
45	49797.0
46	54948.0
47	59829.5
48	69742.0
49	74773.0
50	79951.0
51	85129.0
52	89977.0
53	98764.5
54	102704.0
55	105604.5
56	108505.0
57	108931.5
58	105687.0
59	102016.0
60	96513.5
61	90222.5
62	89434.0
63	72067.5
64	54701.0
65	46934.5
66	33174.0
67	27180.0
68	22531.5
69	17883.0
70	14708.0
71	9325.5
72	7118.0
73	5683.5
74	4249.0
75	3379.0
76	1955.5
77	1402.0
78	1093.5
79	785.0
80	600.5
81	314.0
82	212.0
83	158.5
84	83.5
85	62.0
86	46.5
87	31.0
88	26.0
89	16.5
90	12.0
91	12.5
92	13.0
93	10.0
94	7.0
95	7.0
96	7.5
97	8.0
98	11.0
99	11.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.032018573807744394
3	2.2762019294605022E-4
4	7.58733976486834E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.1001111545275553
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1317985.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.68448264257968
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.3943493328714	60.788948699549
2	13.664252183864523	20.41015210099323
3	3.020318176827537	6.767127013570323
4	0.8153986440188042	2.4359050350402156
5	0.3105321466314744	1.1595966357530678
6	0.16476155869801237	0.7383079062447647
7	0.10673803819883924	0.5580172612814951
8	0.07927249881160107	0.47363404492231514
9	0.05823673726476626	0.39144425340698175
>10	0.38401237685765877	4.6913741137562734
>50	0.0015685714238141354	0.07337731545844689
>100	4.477876252921526E-4	0.08243647027223007
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	1.1194690632303815E-4	1.4296791497516572
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACTGCTCATCTGGGGGGGGGGGGGGG	17100	1.2974350997924864	TruSeq Adapter, Index 7 (97% over 35bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5385607575200022	0.0	0.0	0.0	0.0
2	1.5410645796424087	0.0	0.0	0.0	0.0
3	1.54387189535541	0.0	0.0	0.0	0.0
4	1.5475896918401955	0.0	0.0	0.0	7.58733976486834E-5
5	1.5519903489038192	0.0	0.0	0.0	7.58733976486834E-5
6	1.560639916235769	0.0	0.0	0.0	7.58733976486834E-5
7	1.5686066229888807	0.0	0.0	0.0	7.58733976486834E-5
8	1.6455422482046458	0.0	0.0	0.0	7.58733976486834E-5
9	1.65510229630838	0.0	0.0	0.0	7.58733976486834E-5
10	1.6630690030614916	0.0	0.0	0.0	1.517467952973668E-4
11	1.6755122402758758	0.0	0.0	0.0	3.79366988243417E-4
12	1.7018403092599688	0.0	0.0	0.0	3.79366988243417E-4
13	1.7472884744515302	0.0	0.0	0.0	3.79366988243417E-4
14	1.8076836989798821	0.0	0.0	0.0	3.79366988243417E-4
15	1.8905374492122444	0.0	0.0	0.0	3.79366988243417E-4
16	1.9789299574729606	0.0	0.0	0.0	3.79366988243417E-4
17	2.0795380827551146	0.0	0.0	0.0	3.79366988243417E-4
18	2.1934240526257884	0.0	0.0	0.0	3.79366988243417E-4
19	2.355337883208079	0.0	0.0	0.0	3.79366988243417E-4
20	2.5572369943512254	0.0	0.0	0.0	3.79366988243417E-4
21	2.8311399598629725	0.0	0.0	0.0	3.79366988243417E-4
22	3.1789436146845373	0.0	7.58733976486834E-5	0.0	4.5524038589210043E-4
23	3.623334104712876	0.0	7.58733976486834E-5	0.0	4.5524038589210043E-4
24	4.199744306649924	0.0	1.517467952973668E-4	0.0	4.5524038589210043E-4
25	4.928811784656123	0.0	1.517467952973668E-4	0.0	4.5524038589210043E-4
26	5.789140240594544	0.0	1.517467952973668E-4	0.0	4.5524038589210043E-4
27	6.830806116913318	0.0	1.517467952973668E-4	0.0	4.5524038589210043E-4
28	7.993034822095851	0.0	1.517467952973668E-4	0.0	4.5524038589210043E-4
29	9.288952453935364	0.0	1.517467952973668E-4	0.0	4.5524038589210043E-4
30	10.749818852263115	0.0	1.517467952973668E-4	0.0	4.5524038589210043E-4
31	12.35954885677758	0.0	1.517467952973668E-4	0.0	4.5524038589210043E-4
32	14.109037659760922	0.0	1.517467952973668E-4	0.0	4.5524038589210043E-4
33	15.966949547984234	0.0	1.517467952973668E-4	0.0	4.5524038589210043E-4
34	17.954908439777387	0.0	1.517467952973668E-4	0.0	4.5524038589210043E-4
35	20.02124455134163	0.0	1.517467952973668E-4	0.0	4.5524038589210043E-4
36	22.175138563792455	0.0	1.517467952973668E-4	0.0	4.5524038589210043E-4
37	24.386013497877443	0.0	1.517467952973668E-4	0.0	4.5524038589210043E-4
38	26.614794553807517	0.0	1.517467952973668E-4	0.0	4.5524038589210043E-4
39	28.888492661145612	0.0	1.517467952973668E-4	0.0	5.311137835407838E-4
40	31.309385159922154	0.0	1.517467952973668E-4	0.0	5.311137835407838E-4
41	33.73187100004932	0.0	1.517467952973668E-4	0.0	5.311137835407838E-4
42	36.186299540586575	0.0	1.517467952973668E-4	0.0	5.311137835407838E-4
43	38.6573443552089	0.0	1.517467952973668E-4	0.0	5.311137835407838E-4
44	41.137873344537304	0.0	1.517467952973668E-4	0.0	5.311137835407838E-4
45	43.63168017845423	0.0	1.517467952973668E-4	0.0	5.311137835407838E-4
46	46.01698805373354	0.0	1.517467952973668E-4	0.0	5.311137835407838E-4
47	48.34834994328464	0.0	1.517467952973668E-4	0.0	5.311137835407838E-4
48	50.59723744959161	0.0	1.517467952973668E-4	0.0	5.311137835407838E-4
49	52.78732307272086	0.0	1.517467952973668E-4	0.0	5.311137835407838E-4
50	54.93507133996214	0.0	1.517467952973668E-4	0.0	5.311137835407838E-4
>>END_MODULE