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"TTCTC":94146, "TTCTG":70205, "TTCCA":77884, "TTCCT":115628, "TTCCC":82547, "TTCCG":64947, "TTCGA":51584, "TTCGT":49329, "TTCGC":54992, "TTCGG":59138, "TTGAA":62267, "TTGAT":63381, "TTGAC":45174, "TTGAG":65141, "TTGTA":34076, "TTGTT":78721, "TTGTC":55299, "TTGTG":56986, "TTGCA":40271, "TTGCT":66194, "TTGCC":75955, "TTGCG":43699, "TTGGA":65584, "TTGGT":81906, "TTGGC":76564, "TTGGG":80968, "TCAAA":60193, "TCAAT":47127, "TCAAC":63326, "TCAAG":64812, "TCATA":30964, "TCATT":47607, "TCATC":110224, "TCATG":45719, "TCACA":37735, "TCACT":121767, "TCACC":87469, "TCACG":42983, "TCAGA":104374, "TCAGT":29552, "TCAGC":59563, "TCAGG":52175, "TCTAA":24244, "TCTAT":31003, "TCTAC":39716, "TCTAG":27166, "TCTTA":26629, "TCTTT":84841, "TCTTC":116645, "TCTTG":65492, "TCTCA":44668, "TCTCT":67757, "TCTCC":115727, "TCTCG":54287, "TCTGA":431925, "TCTGT":37421, "TCTGC":68827, "TCTGG":86785, "TCCAA":69610, "TCCAT":63309, "TCCAC":77175, "TCCAG":291618, "TCCTA":31657, "TCCTT":95089, "TCCTC":200534, "TCCTG":119748, "TCCCA":75237, "TCCCT":100999, "TCCCC":107220, "TCCCG":64643, "TCCGA":43807, "TCCGT":42589, "TCCGC":68991, "TCCGG":66377, "TCGAA":53098, "TCGAT":56993, "TCGAC":55536, "TCGAG":61192, "TCGTA":32856, "TCGTT":45599, "TCGTC":75434, "TCGTG":43770, "TCGCA":34312, "TCGCT":47204, "TCGCC":95531, "TCGCG":45062, "TCGGA":885132, "TCGGT":53368, "TCGGC":83136, "TCGGG":52366, "TGAAA":63658, "TGAAT":42514, "TGAAC":380758, "TGAAG":81819, "TGATA":37428, "TGATT":53871, "TGATC":59457, "TGATG":72420, "TGACA":34162, "TGACT":29630, "TGACC":56571, "TGACG":44631, "TGAGA":89044, "TGAGT":31468, "TGAGC":44259, "TGAGG":62246, "TGTAA":21166, "TGTAT":22081, "TGTAC":25085, "TGTAG":42086, "TGTTA":32586, "TGTTT":61115, "TGTTC":59461, "TGTTG":82570, "TGTCA":33353, "TGTCT":40215, "TGTCC":51019, "TGTCG":44681, "TGTGA":33305, "TGTGT":40720, "TGTGC":39999, "TGTGG":73437, "TGCAA":40288, "TGCAT":36003, "TGCAC":41325, "TGCAG":72238, "TGCTA":30252, "TGCTT":72323, "TGCTC":116213, "TGCTG":127826, "TGCCA":64371, "TGCCT":60722, 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"CTCGC":57037, "CTCGG":59892, "CTGAA":419870, "CTGAT":50294, "CTGAC":39944, "CTGAG":52573, "CTGTA":27817, "CTGTT":65508, "CTGTC":41386, "CTGTG":42608, "CTGCA":68406, "CTGCT":182196, "CTGCC":94274, "CTGCG":56915, "CTGGA":81255, "CTGGT":81171, "CTGGC":80380, "CTGGG":97048, "CCAAA":101265, "CCAAT":52937, "CCAAC":112578, "CCAAG":95503, "CCATA":44089, "CCATT":53397, "CCATC":89277, "CCATG":55951, "CCACA":97155, "CCACT":46867, "CCACC":193513, "CCACG":64340, "CCAGA":174451, "CCAGT":222500, "CCAGC":122367, "CCAGG":84148, "CCTAA":29149, "CCTAT":28931, "CCTAC":38875, "CCTAG":42629, "CCTTA":31941, "CCTTT":76304, "CCTTC":114009, "CCTTG":76828, "CCTCA":82268, "CCTCT":78033, "CCTCC":258832, "CCTCG":79637, "CCTGA":62038, "CCTGT":60466, "CCTGC":133601, "CCTGG":85303, "CCCAA":110410, "CCCAT":63454, "CCCAC":120553, "CCCAG":118455, "CCCTA":41423, "CCCTT":72511, "CCCTC":137860, "CCCTG":73212, "CCCCA":130340, "CCCCT":93143, "CCCCC":123366, "CCCCG":79681, "CCCGA":65986, "CCCGT":47779, "CCCGC":97173, "CCCGG":70853, "CCGAA":68179, "CCGAT":56954, "CCGAC":63359, "CCGAG":77861, "CCGTA":35509, "CCGTT":45997, "CCGTC":67558, "CCGTG":46503, "CCGCA":75468, "CCGCT":67106, "CCGCC":147128, "CCGCG":68696, "CCGGA":68374, "CCGGT":63327, "CCGGC":97685, "CCGGG":66744, "CGAAA":61979, "CGAAT":38783, "CGAAC":50852, "CGAAG":80559, "CGATA":39707, "CGATT":46361, "CGATC":63429, "CGATG":69940, "CGACA":53449, "CGACT":39497, "CGACC":73332, "CGACG":71515, "CGAGA":103259, "CGAGT":30565, "CGAGC":56167, "CGAGG":72306, "CGTAA":28346, "CGTAT":20941, "CGTAC":22755, "CGTAG":42493, "CGTTA":22580, "CGTTT":41963, "CGTTC":48679, "CGTTG":49807, "CGTCA":49880, "CGTCT":504823, "CGTCC":62121, "CGTCG":69519, "CGTGA":41319, "CGTGT":28842, "CGTGC":38416, "CGTGG":56164, "CGCAA":50491, "CGCAT":35332, "CGCAC":49651, "CGCAG":71385, "CGCTA":25550, "CGCTT":50413, "CGCTC":55672, "CGCTG":67494, "CGCCA":96351, "CGCCT":80940, "CGCCC":96548, "CGCCG":117260, "CGCGA":50070, "CGCGT":33049, "CGCGC":60009, "CGCGG":65757, "CGGAA":880549, "CGGAT":47851, "CGGAC":37693, "CGGAG":77295, "CGGTA":38321, "CGGTT":50320, "CGGTC":55452, "CGGTG":78823, "CGGCA":83000, "CGGCT":72828, "CGGCC":85714, "CGGCG":110991, "CGGGA":61088, "CGGGT":53149, "CGGGC":64167, "CGGGG":67046, "GAAAA":95851, "GAAAT":61448, "GAAAC":58409, "GAAAG":80319, "GAATA":39255, "GAATT":47357, "GAATC":48547, "GAATG":42562, "GAACA":62121, "GAACT":350385, "GAACC":60541, "GAACG":45747, "GAAGA":931625, "GAAGT":46689, "GAAGC":78474, "GAAGG":94585, "GATAA":43776, "GATAT":38036, "GATAC":28123, "GATAG":52557, "GATTA":33704, "GATTT":65354, "GATTC":51416, "GATTG":51442, "GATCA":62775, "GATCT":57961, "GATCC":53291, "GATCG":951857, "GATGA":87674, "GATGT":47633, "GATGC":55901, "GATGG":79574, "GACAA":58380, "GACAT":37284, "GACAC":34588, "GACAG":56423, "GACTA":24024, "GACTT":45716, "GACTC":29722, "GACTG":35621, "GACCA":73288, "GACCT":49809, "GACCC":59518, "GACCG":55001, "GACGA":77124, "GACGT":34207, "GACGC":47450, "GACGG":57291, "GAGAA":88355, "GAGAT":261610, "GAGAC":53530, "GAGAG":75473, "GAGTA":25661, "GAGTT":44213, "GAGTC":28063, "GAGTG":30778, "GAGCA":757764, "GAGCT":41141, "GAGCC":54109, "GAGCG":47622, "GAGGA":182804, "GAGGT":60095, "GAGGC":69953, "GAGGG":97538, "GTAAA":29655, "GTAAT":25942, "GTAAC":23483, "GTAAG":29574, "GTATA":17036, "GTATT":29362, "GTATC":25957, "GTATG":25784, "GTACA":26185, "GTACT":21603, "GTACC":29062, "GTACG":23021, "GTAGA":95753, "GTAGT":26318, "GTAGC":33813, "GTAGG":30759, "GTTAA":20475, "GTTAT":25457, "GTTAC":26616, "GTTAG":36281, "GTTTA":30961, "GTTTT":71688, "GTTTC":59200, "GTTTG":65473, "GTTCA":49383, "GTTCT":53817, "GTTCC":68663, "GTTCG":46427, "GTTGA":62764, "GTTGT":67915, "GTTGC":57582, "GTTGG":93037, "GTCAA":45315, "GTCAT":39814, "GTCAC":141632, "GTCAG":54442, "GTCTA":26435, "GTCTT":51764, "GTCTC":54137, "GTCTG":468032, "GTCCA":58164, "GTCCT":56702, "GTCCC":49387, "GTCCG":37538, "GTCGA":56252, "GTCGT":52426, "GTCGC":52179, "GTCGG":54781, "GTGAA":54364, "GTGAT":49216, "GTGAC":32157, "GTGAG":53646, "GTGTA":22720, "GTGTT":49668, "GTGTC":30385, "GTGTG":50744, "GTGCA":38543, "GTGCT":53876, "GTGCC":47823, "GTGCG":41010, "GTGGA":71431, "GTGGT":100770, "GTGGC":67305, "GTGGG":85350, "GCAAA":60700, "GCAAT":41655, "GCAAC":57827, "GCAAG":72529, "GCATA":30943, "GCATT":41932, "GCATC":53363, "GCATG":41066, "GCACA":669573, "GCACT":27883, "GCACC":81703, "GCACG":39575, "GCAGA":140450, "GCAGT":39688, "GCAGC":105851, "GCAGG":111953, "GCTAA":22955, "GCTAT":27769, "GCTAC":37288, "GCTAG":38455, "GCTTA":29430, "GCTTT":62750, "GCTTC":86127, "GCTTG":67035, "GCTCA":83824, "GCTCT":47548, "GCTCC":83369, "GCTCG":57284, "GCTGA":64383, "GCTGT":55138, "GCTGC":109947, "GCTGG":111223, "GCCAA":84151, "GCCAT":57564, "GCCAC":75319, "GCCAG":109835, "GCCTA":33247, "GCCTT":64697, "GCCTC":79357, "GCCTG":80034, "GCCCA":89841, "GCCCT":70809, "GCCCC":81445, "GCCCG":69713, "GCCGA":90678, "GCCGT":65406, "GCCGC":107661, "GCCGG":93230, "GCGAA":55477, "GCGAT":55615, "GCGAC":54074, "GCGAG":64855, "GCGTA":24461, "GCGTT":40615, "GCGTC":44237, "GCGTG":43226, "GCGCA":53369, "GCGCT":48980, "GCGCC":75146, "GCGCG":56147, "GCGGA":68031, "GCGGT":68127, "GCGGC":97990, "GCGGG":80823, "GGAAA":84349, "GGAAT":53362, "GGAAC":60800, "GGAAG":905411, "GGATA":47266, "GGATT":60925, "GGATC":49451, "GGATG":73422, "GGACA":53495, "GGACT":38187, "GGACC":44868, "GGACG":56631, "GGAGA":185310, "GGAGT":42349, "GGAGC":72720, "GGAGG":206370, "GGTAA":33579, "GGTAT":31256, "GGTAC":28823, "GGTAG":61798, "GGTTA":33167, "GGTTT":77852, "GGTTC":55924, "GGTTG":98622, "GGTCA":56650, "GGTCT":55704, "GGTCC":44289, "GGTCG":62611, "GGTGA":82270, "GGTGT":63355, "GGTGC":70917, "GGTGG":150080, "GGCAA":73237, "GGCAT":53443, "GGCAC":47669, "GGCAG":107348, "GGCTA":39244, "GGCTT":73613, "GGCTC":55725, "GGCTG":91746, "GGCCA":81478, "GGCCT":62583, "GGCCC":58053, "GGCCG":82022, "GGCGA":97465, "GGCGT":56731, "GGCGC":71986, "GGCGG":124848, "GGGAA":70382, "GGGAT":58184, "GGGAC":44784, "GGGAG":126557, "GGGTA":35408, "GGGTT":78906, "GGGTC":51031, "GGGTG":87665, "GGGCA":74194, 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"GGGCT":69136, "GGGCC":58892, "GGGCG":96703, "GGGGA":158896, "GGGGT":103239, "GGGGC":82046, "GGGGG":198425 }, "overrepresented_sequences": { } }, "merged_and_filtered": { "total_reads": 1065002, "total_bases": 54113512, "q20_bases": 53815329, "q30_bases": 52237429, "total_cycles": 91, "quality_curves": { 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"CTTGA":53396, "CTTGT":53482, "CTTGC":54464, "CTTGG":63859, "CTCAA":53371, "CTCAT":44418, "CTCAC":44408, "CTCAG":37864, "CTCTA":26739, "CTCTT":67647, "CTCTC":61892, "CTCTG":46937, "CTCCA":88079, "CTCCT":184671, "CTCCC":124206, "CTCCG":62022, "CTCGA":50906, "CTCGT":47238, "CTCGC":51293, "CTCGG":53653, "CTGAA":47979, "CTGAT":45600, "CTGAC":35612, "CTGAG":36167, "CTGTA":22311, "CTGTT":59907, "CTGTC":37392, "CTGTG":38407, "CTGCA":56493, "CTGCT":134025, "CTGCC":84878, "CTGCG":50711, "CTGGA":68796, "CTGGT":73756, "CTGGC":72000, "CTGGG":68180, "CCAAA":87913, "CCAAT":48824, "CCAAC":102555, "CCAAG":65646, "CCATA":35386, "CCATT":49135, "CCATC":80962, "CCATG":50758, "CCACA":81491, "CCACT":42617, "CCACC":174933, "CCACG":57471, "CCAGA":61431, "CCAGT":50489, "CCAGC":109271, "CCAGG":75142, "CCTAA":23467, "CCTAT":26636, "CCTAC":34979, "CCTAG":15883, "CCTTA":22381, "CCTTT":70140, "CCTTC":103151, "CCTTG":70343, "CCTCA":65186, "CCTCT":70976, "CCTCC":226760, "CCTCG":71705, "CCTGA":51988, "CCTGT":55114, "CCTGC":120873, "CCTGG":76980, "CCCAA":93598, "CCCAT":57912, "CCCAC":108443, "CCCAG":76698, "CCCTA":30479, "CCCTT":65690, "CCCTC":117886, "CCCTG":66166, "CCCCA":106275, "CCCCT":82469, "CCCCC":98207, "CCCCG":70711, "CCCGA":53818, "CCCGT":41883, "CCCGC":86147, "CCCGG":63257, "CCGAA":55338, "CCGAT":50567, "CCGAC":56320, "CCGAG":54637, "CCGTA":27757, "CCGTT":41498, "CCGTC":59693, "CCGTG":41928, "CCGCA":60345, "CCGCT":60338, "CCGCC":130123, "CCGCG":60709, "CCGGA":56586, "CCGGT":56487, "CCGGC":86643, "CCGGG":59163, "CGAAA":51020, "CGAAT":34955, "CGAAC":44136, "CGAAG":57583, "CGATA":31274, "CGATT":42082, "CGATC":54965, "CGATG":62942, "CGACA":42941, "CGACT":36102, "CGACC":65336, "CGACG":63367, "CGAGA":48563, "CGAGT":27499, "CGAGC":49063, "CGAGG":64021, "CGTAA":23358, "CGTAT":19133, "CGTAC":20572, "CGTAG":25486, "CGTTA":17484, "CGTTT":37990, "CGTTC":43704, "CGTTG":45039, "CGTCA":39570, "CGTCT":41071, "CGTCC":56104, "CGTCG":60954, "CGTGA":34849, "CGTGT":25335, "CGTGC":34290, "CGTGG":50223, "CGCAA":41933, "CGCAT":31764, "CGCAC":43154, "CGCAG":47280, "CGCTA":19012, "CGCTT":45608, "CGCTC":49592, "CGCTG":59867, "CGCCA":77586, "CGCCT":72800, "CGCCC":85375, "CGCCG":103267, "CGCGA":41435, "CGCGT":29488, "CGCGC":52435, "CGCGG":57986, "CGGAA":50868, "CGGAT":43197, "CGGAC":33121, "CGGAG":54648, "CGGTA":29170, "CGGTT":46041, "CGGTC":49586, "CGGTG":70012, "CGGCA":66460, "CGGCT":65933, "CGGCC":76209, "CGGCG":97189, "CGGGA":49989, "CGGGT":47772, "CGGGC":56391, "CGGGG":59125, "GAAAA":82795, "GAAAT":55982, "GAAAC":53403, "GAAAG":59285, "GAATA":33619, "GAATT":43848, "GAATC":44308, "GAATG":39153, "GAACA":50606, "GAACT":42864, "GAACC":54308, "GAACG":40777, "GAAGA":94063, "GAAGT":42307, "GAAGC":71218, "GAAGG":85536, "GATAA":37999, "GATAT":35020, "GATAC":25895, "GATAG":30573, "GATTA":27497, "GATTT":60279, "GATTC":47257, "GATTG":46609, "GATCA":50469, "GATCT":52514, "GATCC":47838, "GATCG":49535, "GATGA":75927, "GATGT":43683, "GATGC":50557, "GATGG":72028, "GACAA":50180, "GACAT":33617, "GACAC":31220, "GACAG":34222, "GACTA":18819, "GACTT":42456, "GACTC":26693, "GACTG":31698, "GACCA":60435, "GACCT":44928, "GACCC":53618, "GACCG":48959, "GACGA":65403, "GACGT":29985, "GACGC":42335, "GACGG":51733, "GAGAA":76229, "GAGAT":56022, "GAGAC":48452, "GAGAG":52318, "GAGTA":20397, "GAGTT":40009, "GAGTC":25644, "GAGTG":27840, "GAGCA":64037, "GAGCT":36733, "GAGCC":48498, "GAGCG":41318, "GAGGA":154620, "GAGGT":53829, "GAGGC":62793, "GAGGG":85766, "GTAAA":26425, "GTAAT":23968, "GTAAC":21185, "GTAAG":17817, "GTATA":13954, "GTATT":27267, "GTATC":23741, "GTATG":23975, "GTACA":21913, "GTACT":19899, "GTACC":26553, "GTACG":20702, "GTAGA":30011, "GTAGT":24140, "GTAGC":30949, "GTAGG":27402, "GTTAA":17372, "GTTAT":23742, "GTTAC":24624, "GTTAG":17047, "GTTTA":24580, "GTTTT":66180, "GTTTC":53944, "GTTTG":60432, "GTTCA":41501, "GTTCT":49416, "GTTCC":61842, "GTTCG":41589, "GTTGA":53748, "GTTGT":61991, "GTTGC":52574, "GTTGG":85376, "GTCAA":38584, "GTCAT":35947, "GTCAC":31085, "GTCAG":31334, "GTCTA":19563, "GTCTT":47274, "GTCTC":50924, "GTCTG":37436, "GTCCA":48769, "GTCCT":51633, "GTCCC":45423, "GTCCG":33447, "GTCGA":46575, "GTCGT":46658, "GTCGC":46433, "GTCGG":48352, "GTGAA":46018, "GTGAT":43908, "GTGAC":28953, "GTGAG":36702, "GTGTA":17427, "GTGTT":45533, "GTGTC":27386, "GTGTG":45274, "GTGCA":31203, "GTGCT":48463, "GTGCC":42684, "GTGCG":36233, "GTGGA":59285, "GTGGT":91088, "GTGGC":60362, "GTGGG":75396, "GCAAA":51021, "GCAAT":38056, "GCAAC":52512, "GCAAG":49572, "GCATA":24238, "GCATT":38920, "GCATC":48496, "GCATG":37334, "GCACA":38501, "GCACT":24906, "GCACC":72725, "GCACG":34707, "GCAGA":52084, "GCAGT":36250, "GCAGC":95990, "GCAGG":99248, "GCTAA":18060, "GCTAT":25557, "GCTAC":34003, "GCTAG":16290, "GCTTA":22109, "GCTTT":57826, "GCTTC":79442, "GCTTG":61399, "GCTCA":44224, "GCTCT":43602, "GCTCC":76213, "GCTCG":50588, "GCTGA":53330, "GCTGT":49712, "GCTGC":99873, "GCTGG":99642, "GCCAA":69583, "GCCAT":52335, "GCCAC":67984, "GCCAG":69476, "GCCTA":23902, "GCCTT":59265, "GCCTC":72661, "GCCTG":71864, "GCCCA":72263, "GCCCT":64211, "GCCCC":72802, "GCCCG":60973, "GCCGA":74610, "GCCGT":58710, "GCCGC":94545, "GCCGG":81930, "GCGAA":45184, "GCGAT":49428, "GCGAC":47819, "GCGAG":44705, "GCGTA":18639, "GCGTT":36522, "GCGTC":39174, "GCGTG":38245, "GCGCA":42051, "GCGCT":43418, "GCGCC":66594, "GCGCG":48640, "GCGGA":55351, "GCGGT":61109, "GCGGC":86799, "GCGGG":71004, "GGAAA":69513, "GGAAT":49117, "GGAAC":55100, "GGAAG":84158, "GGATA":37809, "GGATT":55959, "GGATC":44602, "GGATG":66798, "GGACA":42951, "GGACT":34442, "GGACC":40437, "GGACG":49812, "GGAGA":97112, "GGAGT":38438, "GGAGC":65274, "GGAGG":180408, "GGTAA":27046, "GGTAT":28960, "GGTAC":26340, "GGTAG":34730, "GGTTA":24509, "GGTTT":71921, "GGTTC":51202, "GGTTG":90061, "GGTCA":44160, "GGTCT":50931, "GGTCC":39992, "GGTCG":55293, "GGTGA":67351, "GGTGT":57677, "GGTGC":63040, "GGTGG":133707, "GGCAA":60207, "GGCAT":48969, "GGCAC":42646, "GGCAG":70640, "GGCTA":29786, "GGCTT":67458, "GGCTC":50637, "GGCTG":82355, "GGCCA":64490, "GGCCT":56447, "GGCCC":51825, "GGCCG":71650, "GGCGA":80520, "GGCGT":50385, "GGCGC":63200, "GGCGG":109609, "GGGAA":57465, "GGGAT":52786, "GGGAC":39785, "GGGAG":93076, "GGGTA":26491, "GGGTT":72487, "GGGTC":46356, "GGGTG":77308, "GGGCA":59017, "GGGCT":56892, "GGGCC":48275, "GGGCG":70244, "GGGGA":73440, "GGGGT":67383, "GGGGC":59895, "GGGGG":69507 }, "overrepresented_sequences": { } }, "command": "/redser4/software/fastp -l 30 -q 25 -y -Y 30 --detect_adapter_for_pe -i /scratch3/cwanket/andy-lake/raw/b2/AM-AL-31B_S33_R1_001.fastq.gz -I /scratch3/cwanket/andy-lake/raw/b2/AM-AL-31B_S33_R2_001.fastq.gz -o AM-AL-31B.trim.R1.fastq.gz -O AM-AL-31B.trim.R2.fastq.gz -m --merged_out AM-AL-31B.trim.merged.fastq.gz " }