##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-31A_S32_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1145144
Sequences flagged as poor quality	0
Sequence length	61
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.44452750047156	40.0	40.0	40.0	40.0	40.0
2	39.145586930551964	40.0	40.0	40.0	40.0	40.0
3	39.10956351340967	40.0	40.0	40.0	40.0	40.0
4	38.97807437317927	40.0	40.0	40.0	40.0	40.0
5	39.03356433776014	40.0	40.0	40.0	40.0	40.0
6	39.15314056572798	40.0	40.0	40.0	40.0	40.0
7	39.17035412140307	40.0	40.0	40.0	40.0	40.0
8	39.154670504320855	40.0	40.0	40.0	40.0	40.0
9	39.179865588956496	40.0	40.0	40.0	40.0	40.0
10	39.18616348686279	40.0	40.0	40.0	40.0	40.0
11	39.1909069951028	40.0	40.0	40.0	40.0	40.0
12	39.18896837428306	40.0	40.0	40.0	40.0	40.0
13	39.01267613505376	40.0	40.0	40.0	40.0	40.0
14	39.18724981312394	40.0	40.0	40.0	40.0	40.0
15	39.091712483320876	40.0	40.0	40.0	40.0	40.0
16	39.27974473079368	40.0	40.0	40.0	40.0	40.0
17	39.22723430415738	40.0	40.0	40.0	40.0	40.0
18	39.312152882082955	40.0	40.0	40.0	40.0	40.0
19	39.260348043564825	40.0	40.0	40.0	40.0	40.0
20	39.26349175300224	40.0	40.0	40.0	40.0	40.0
21	39.248283185346125	40.0	40.0	40.0	40.0	40.0
22	39.310975737549164	40.0	40.0	40.0	40.0	40.0
23	39.302906883326465	40.0	40.0	40.0	40.0	40.0
24	39.298310081526864	40.0	40.0	40.0	40.0	40.0
25	39.255384475664194	40.0	40.0	40.0	40.0	40.0
26	39.222193890026055	40.0	40.0	40.0	40.0	40.0
27	39.23400375847928	40.0	40.0	40.0	40.0	40.0
28	39.24940793472262	40.0	40.0	40.0	40.0	40.0
29	39.24524775923377	40.0	40.0	40.0	40.0	40.0
30	39.24865693746813	40.0	40.0	40.0	40.0	40.0
31	39.2009494002501	40.0	40.0	40.0	40.0	40.0
32	39.2259803133929	40.0	40.0	40.0	40.0	40.0
33	39.21914798488225	40.0	40.0	40.0	40.0	40.0
34	39.20610857673795	40.0	40.0	40.0	40.0	40.0
35	39.2277547627198	40.0	40.0	40.0	40.0	40.0
36	39.180165987858295	40.0	40.0	40.0	40.0	40.0
37	39.20245838078006	40.0	40.0	40.0	40.0	40.0
38	39.197508784921375	40.0	40.0	40.0	40.0	40.0
39	37.960848591967476	40.0	40.0	40.0	40.0	40.0
40	39.197023256463815	40.0	40.0	40.0	40.0	40.0
41	39.209392006594804	40.0	40.0	40.0	40.0	40.0
42	39.18236658446449	40.0	40.0	40.0	40.0	40.0
43	39.19956267508715	40.0	40.0	40.0	40.0	40.0
44	39.16694494316872	40.0	40.0	40.0	40.0	40.0
45	39.0130638592177	40.0	40.0	40.0	40.0	40.0
46	39.0254570604221	40.0	40.0	40.0	40.0	40.0
47	38.96917068945041	40.0	40.0	40.0	40.0	40.0
48	39.20734859546048	40.0	40.0	40.0	40.0	40.0
49	39.15142899058983	40.0	40.0	40.0	40.0	40.0
50	39.141407543505444	40.0	40.0	40.0	40.0	40.0
51	39.00451646255842	40.0	40.0	40.0	40.0	40.0
52	39.08952935176711	40.0	40.0	40.0	40.0	40.0
53	38.89859091956994	40.0	40.0	40.0	40.0	40.0
54	38.71036306350992	40.0	40.0	40.0	40.0	40.0
55	38.867223685405506	40.0	40.0	40.0	40.0	40.0
56	38.810888412287014	40.0	40.0	40.0	40.0	40.0
57	38.61581774868488	40.0	40.0	40.0	40.0	40.0
58	38.52052842262633	40.0	40.0	40.0	40.0	40.0
59	38.43990275458807	40.0	40.0	40.0	40.0	40.0
60	38.40161935966132	40.0	40.0	40.0	40.0	40.0
61	38.28465241052653	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.04650766398405892
1101	2	0.002632439095805239
1101	3	0.01891496788728375
1101	4	0.05371788917453557
1101	5	-0.03160854114003797
1101	6	0.021541838794931323
1101	7	0.16271990543770443
1101	8	0.04163396145788312
1101	9	0.010297271781382733
1101	10	-0.0036261430254214133
1101	11	0.03938531816989155
1101	12	-0.08424444237284234
1101	13	-0.031387352777173305
1101	14	0.03857691541472974
1101	15	0.08545661977827024
1101	16	-0.04223348138268079
1101	17	0.0428343316462545
1101	18	0.06761589491367914
1101	19	-0.014186676635254969
1101	20	0.03789863267216731
1101	21	0.007981292107750448
1101	22	-0.06420013350604847
1101	23	0.0248618541076695
1101	24	0.009651590108042285
1101	25	0.036027110724916156
1101	26	0.032513681472359224
1101	27	-0.034693833928784557
1101	28	-0.03471599892891675
1101	29	0.0607363920885291
1101	30	6.120045240933791E-4
1101	31	0.027752705326825833
1101	32	-0.014874027988355465
1101	33	-0.01514900300351485
1101	34	0.010745941298196726
1101	35	0.04316744621345947
1101	36	-0.058187557127467926
1101	37	-0.0692942962515275
1101	38	-0.12926443499647888
1101	39	1.2661311423025197
1101	40	-0.1078057450777905
1101	41	-0.04791616520854802
1101	42	-0.04529586918680195
1101	43	-0.12948431407664174
1101	44	-0.12420772274668224
1101	45	-0.09144124439406909
1101	46	0.018699825971225437
1101	47	-0.00533867118373621
1101	48	-0.09317904461189386
1101	49	-0.031055259824789516
1101	50	-0.1571729028848381
1101	51	-0.145464648318665
1101	52	-0.028010961021976755
1101	53	-0.021139500543341683
1101	54	-0.08604227891301264
1101	55	-0.003582123119286962
1101	56	0.029029652888269197
1101	57	0.15831610449212974
1101	58	0.1267678067230733
1101	59	0.07200532103493629
1101	60	0.09677315104142536
1101	61	0.060148390875383484
1102	1	0.12079436757507267
1102	2	0.04316711071670909
1102	3	0.09166826937194372
1102	4	-0.023973564765533695
1102	5	-0.024338036547717934
1102	6	-0.036300494200617095
1102	7	0.016296446800765807
1102	8	-0.11680513198097486
1102	9	-0.023642473107543083
1102	10	-0.07213295510217677
1102	11	-0.006409209649440584
1102	12	0.021403066835929963
1102	13	-0.10849973997979845
1102	14	-0.017544302555641877
1102	15	-0.05766937549268647
1102	16	-0.026872127653327027
1102	17	0.00754343103662336
1102	18	-0.030182042965329003
1102	19	-0.03060965271658489
1102	20	-0.08225771534694104
1102	21	-0.11096866687501716
1102	22	0.024702695878374925
1102	23	-0.061772963888437005
1102	24	0.01502404261653112
1102	25	-0.07983449232483508
1102	26	0.06149918871873439
1102	27	-0.06335763743214073
1102	28	0.05006902254481105
1102	29	-0.047018276637039946
1102	30	0.015892932317690622
1102	31	-0.07464559607740995
1102	32	0.032529018546092914
1102	33	0.04213034844173791
1102	34	0.0625884994863668
1102	35	-0.06061418128577856
1102	36	-0.09309241324547202
1102	37	0.01886861452393873
1102	38	-0.055336914849782204
1102	39	1.2101546910165055
1102	40	0.04085355457316098
1102	41	-0.03010986303222296
1102	42	0.10074154495851673
1102	43	0.06548423938247794
1102	44	0.053662276770765516
1102	45	-0.06176407410018925
1102	46	0.030120308848545108
1102	47	-0.04053306067046236
1102	48	-0.01178799762706717
1102	49	-0.04826641007652199
1102	50	-0.05115543437159431
1102	51	-0.06556952373889402
1102	52	0.08143265235446506
1102	53	-0.012163966113000413
1102	54	-0.1918023844318384
1102	55	-0.08367026994487503
1102	56	-0.09900843683490734
1102	57	-0.03985340121007397
1102	58	-0.0584531233224439
1102	59	-0.06299765472468266
1102	60	-0.08066254842293574
1102	61	-0.07269916980597912
1103	1	0.1628572736950531
1103	2	0.16957707125981614
1103	3	0.09336364455468527
1103	4	-0.029026687336141777
1103	5	0.029686615454217247
1103	6	0.16774958231739845
1103	7	0.006099749054257586
1103	8	0.1902325696427738
1103	9	0.07308314258133919
1103	10	0.011727610984451076
1103	11	0.034054885555590886
1103	12	0.04177812734138797
1103	13	0.173064552265231
1103	14	0.024277804061092922
1103	15	0.04672515432489632
1103	16	0.06922887612612527
1103	17	-0.0019027545525673872
1103	18	0.020448191673622773
1103	19	-0.0379986527019156
1103	20	-0.023079709509083557
1103	21	0.0698766493912899
1103	22	0.0518030298196166
1103	23	0.041081426974379553
1103	24	0.030223747300993864
1103	25	0.09172109691277797
1103	26	0.04500014458275814
1103	27	0.09734730616943921
1103	28	0.10955261150861162
1103	29	0.003152360980486435
1103	30	0.04265345782287966
1103	31	0.13585839606054861
1103	32	0.07669678123757251
1103	33	0.006985844293140531
1103	34	0.0186163099954868
1103	35	0.061356267603606796
1103	36	0.13300120764565548
1103	37	0.08328798213799615
1103	38	0.05372570279972422
1103	39	1.3378028743859431
1103	40	0.11280249041048762
1103	41	0.02568717110018781
1103	42	0.04238372423068881
1103	43	-0.01254335599612233
1103	44	0.11366780171368163
1103	45	0.04652480707441953
1103	46	0.09469613502308505
1103	47	0.18797955624564366
1103	48	0.1397470075311773
1103	49	0.05768048202084941
1103	50	0.1221688699989798
1103	51	0.12096452799999469
1103	52	-3.0716683810538825E-4
1103	53	0.13851418396009763
1103	54	0.11260607997628114
1103	55	0.13501846063837064
1103	56	0.10695214538651499
1103	57	0.11777982080951688
1103	58	0.11307190760256702
1103	59	0.16532794531833872
1103	60	0.15803698757964213
1103	61	0.150199273920272
1104	1	0.004103761462673106
1104	2	-0.07164219556509721
1104	3	-0.034876530633702885
1104	4	-0.06661533809084119
1104	5	-0.04615141810975132
1104	6	0.07308799283357814
1104	7	0.06458580182269458
1104	8	0.050352853325186686
1104	9	-0.02728601481173598
1104	10	0.08474093889389422
1104	11	-0.05101451871377094
1104	12	0.01759198481082791
1104	13	-0.026048422563619056
1104	14	0.024229390975065712
1104	15	0.08515905493374021
1104	16	0.018958128226152837
1104	17	-0.026166540711656694
1104	18	0.01881828444405187
1104	19	-0.013516550928471815
1104	20	0.009157316088348466
1104	21	-0.015992636494374324
1104	22	-0.03486157041008653
1104	23	-0.013663116353200166
1104	24	0.009748056759882218
1104	25	-0.012729070492980554
1104	26	-0.004488990789980107
1104	27	0.01709424274768878
1104	28	0.03163007488213054
1104	29	0.03214022908741043
1104	30	0.015295801430340816
1104	31	0.01255331079870814
1104	32	-0.11525105685932857
1104	33	0.011566513891793306
1104	34	0.10279357374876241
1104	35	0.09384449317570898
1104	36	-0.11197905564845456
1104	37	-0.10106478666424579
1104	38	-0.08762985142963231
1104	39	1.2032333732904021
1104	40	-0.06810472819378077
1104	41	0.0015512685645617807
1104	42	-0.021650955372116698
1104	43	-0.051307013662629686
1104	44	-0.07180455776352801
1104	45	0.06806893484112209
1104	46	-0.01070859228640586
1104	47	0.0473599418208579
1104	48	-0.004492322407351423
1104	49	0.10089418529917538
1104	50	-0.011386087683725066
1104	51	0.007887087767322498
1104	52	0.14080084123439462
1104	53	0.13561457504039254
1104	54	0.1198701742740127
1104	55	0.07887176472492285
1104	56	0.0726080848500601
1104	57	0.11021976726890159
1104	58	0.01894651588284546
1104	59	-0.032856046331467326
1104	60	0.1354837971268239
1104	61	0.13493514552024521
1201	1	-0.027913187637331305
1201	2	0.09013585719807793
1201	3	0.13923216777790515
1201	4	0.17512888179143715
1201	5	-0.07426645746489413
1201	6	0.04451148604677968
1201	7	0.10146751276612065
1201	8	-0.1508735583671168
1201	9	-0.038923400901140326
1201	10	-0.03862751026633049
1201	11	-0.030617416311926604
1201	12	0.005446561181983611
1201	13	-0.0521694145764684
1201	14	-0.11017889535912673
1201	15	-0.010797140134222616
1201	16	0.05948924216120588
1201	17	0.027521233478353224
1201	18	-0.06145164678716952
1201	19	-0.07543906930684585
1201	20	-0.040854443619871006
1201	21	0.042982659348659524
1201	22	0.015646735512277132
1201	23	-0.07248569853111064
1201	24	-0.013318057143798967
1201	25	-0.03725700193572834
1201	26	0.034701266924919594
1201	27	-0.037975212107618006
1201	28	0.016692259206166682
1201	29	-0.0322359896451303
1201	30	0.02248785904966155
1201	31	0.03322166895821965
1201	32	0.0945052446394854
1201	33	0.03625925513156858
1201	34	0.028246624918651264
1201	35	-0.034491837352305765
1201	36	0.01947172643829731
1201	37	-0.03210534355806516
1201	38	0.11574513568987754
1201	39	1.2639435568499593
1201	40	0.12407831289322502
1201	41	0.08552654739741428
1201	42	0.005018596222008398
1201	43	0.10677491479948742
1201	44	0.031110844384848235
1201	45	0.08684696998930974
1201	46	0.0679204986537485
1201	47	0.0471633796776203
1201	48	8.871298480457313E-4
1201	49	0.006133692868672824
1201	50	0.06924219145478361
1201	51	0.08532561692607032
1201	52	-0.0247295828431362
1201	53	0.09699011389471934
1201	54	0.08568317911269219
1201	55	-0.01014487947695386
1201	56	0.10997031463286788
1201	57	0.034717526422674894
1201	58	-0.06902109128075296
1201	59	-0.06581256247613965
1201	60	0.101148321946539
1201	61	0.2800007288573383
1202	1	0.047189034341961644
1202	2	0.06279940228424863
1202	3	-0.04325857627743801
1202	4	0.0921956773715209
1202	5	-0.06517620955895609
1202	6	0.04781018808638038
1202	7	0.013994859451067043
1202	8	0.05912658052245945
1202	9	0.05726405044083549
1202	10	0.15695469615732804
1202	11	0.07539624833128755
1202	12	-0.03059692589015839
1202	13	0.05410858828705045
1202	14	0.025465739687611233
1202	15	2.940136901585788E-4
1202	16	-0.020432896045264215
1202	17	-0.01732486567356517
1202	18	0.02585254691629757
1202	19	0.005391974215321227
1202	20	0.14917516674110942
1202	21	0.021044976561391593
1202	22	0.06134200252897415
1202	23	0.05528733260837981
1202	24	0.0957947864529487
1202	25	-0.018712406360513967
1202	26	0.0466397964905525
1202	27	0.1051437727977671
1202	28	-0.004130722746999993
1202	29	0.05839587876162966
1202	30	0.10980400024330805
1202	31	-0.06185078286240753
1202	32	0.02223218833559315
1202	33	0.02961376240438085
1202	34	-0.020962697648201356
1202	35	0.06380852762858069
1202	36	-0.33991325858328736
1202	37	-0.1303635936360621
1202	38	-0.196802402253482
1202	39	1.0607618506079817
1202	40	-0.16554736775902512
1202	41	-0.21063202088704713
1202	42	-0.17847185407441657
1202	43	-0.13585949483843507
1202	44	-0.11634119652276098
1202	45	-0.16752495269191314
1202	46	0.06151694260277196
1202	47	-0.06291004212986451
1202	48	-0.11600741279232807
1202	49	-0.015669580654027015
1202	50	-0.07823075756064668
1202	51	0.047234927411018646
1202	52	-0.09117973111895594
1202	53	-0.05237173331868661
1202	54	-0.01643500635626083
1202	55	-0.1221729106784224
1202	56	-0.036017561352785776
1202	57	-0.06936124218535866
1202	58	-0.14118721754750396
1202	59	0.020506261974460926
1202	60	0.05731327915137996
1202	61	-0.10364542710490099
1203	1	0.04869391979885762
1203	2	0.10575112804635012
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2403	3	0.05987815033893895
2403	4	0.18364277767262394
2403	5	0.16591286067110644
2403	6	0.07552858584707423
2403	7	0.04929802156858187
2403	8	0.007635800515892299
2403	9	0.0024773600555576536
2403	10	0.09045200726728808
2403	11	0.08386356107570947
2403	12	0.04021178956649152
2403	13	0.07062371197108774
2403	14	0.11388555795869593
2403	15	0.12179682370828004
2403	16	0.04624458526344455
2403	17	0.137656518933845
2403	18	0.07373892325786358
2403	19	0.02336431469500866
2403	20	0.10011124147376194
2403	21	0.10204500998661103
2403	22	0.07870532942360597
2403	23	0.09534362190026258
2403	24	0.06869950833214489
2403	25	0.07888677377104614
2403	26	0.054168136628007346
2403	27	0.0659179008212547
2403	28	0.022537693888210697
2403	29	0.04356857520453872
2403	30	0.06536195450964044
2403	31	0.0037301060091721183
2403	32	-0.03334014797360396
2403	33	0.06050970234447561
2403	34	-0.009966107940286406
2403	35	0.08354780480366486
2403	36	0.011972705616159374
2403	37	0.08373197365887108
2403	38	0.14450218089572786
2403	39	1.239512550927607
2403	40	0.14978735512293184
2403	41	-0.0037152088922383086
2403	42	0.05524278227608903
2403	43	0.10284929823218647
2403	44	0.09046460695352465
2403	45	0.18642294874111087
2403	46	-0.0023920721168977366
2403	47	0.0793611737599349
2403	48	0.03830669409047971
2403	49	0.07604614799576837
2403	50	-0.03944033336397723
2403	51	0.03206171885698694
2403	52	0.02902348193367743
2403	53	0.15097624680159072
2403	54	-0.06783195043280443
2403	55	0.011945618820554671
2403	56	0.07896089164844966
2403	57	-0.020983607151315198
2403	58	0.02788366261818709
2403	59	0.03921518383948808
2403	60	-0.009476437289322348
2403	61	-0.04528499839122446
2404	1	-0.04255936885358835
2404	2	-0.13024503005068055
2404	3	-0.036194816932621166
2404	4	-0.06104044631266703
2404	5	-0.0028702610167243847
2404	6	-0.07438075602878769
2404	7	0.05819038194008641
2404	8	-0.06485961870200185
2404	9	-0.01203359073319632
2404	10	0.06325880821984242
2404	11	-0.045029672049963665
2404	12	-0.1612126519767969
2404	13	-0.06084728535618211
2404	14	-0.01056761681802243
2404	15	-0.015788161906186815
2404	16	-0.013533424376724668
2404	17	-0.012841461095469242
2404	18	-0.12164938720464846
2404	19	0.03189878306920946
2404	20	-0.050419702292145985
2404	21	-0.081878198948381
2404	22	-0.017167657845952533
2404	23	-0.11008073362337001
2404	24	0.007610169856263838
2404	25	-0.13302578693520672
2404	26	0.00912052208020242
2404	27	0.07339533204202553
2404	28	0.02230035498473626
2404	29	0.058817285812288844
2404	30	0.014652425776482403
2404	31	-0.09244646157739567
2404	32	-0.045661678771864445
2404	33	-0.1400413571492578
2404	34	0.10520290890999462
2404	35	-0.05003977835324491
2404	36	-0.030546433364762038
2404	37	-0.0753965404912833
2404	38	-0.03611806163260667
2404	39	1.244044907739017
2404	40	-0.03869834889693635
2404	41	-0.019443306412156858
2404	42	0.05731730009902947
2404	43	-0.04630438922481517
2404	44	-0.04059450063650161
2404	45	0.13833136857416406
2404	46	-0.119052619562936
2404	47	-0.007233620449810019
2404	48	0.05486673353394167
2404	49	-0.013576286934998905
2404	50	0.1152961553105456
2404	51	-0.10371277276763635
2404	52	-0.06570048366034342
2404	53	0.151619510575685
2404	54	0.018394591748140954
2404	55	-0.030288462323916576
2404	56	0.016518156330143086
2404	57	0.03309136211785102
2404	58	0.01718457554102315
2404	59	0.029912189492144137
2404	60	-0.026225782762935523
2404	61	0.13423488321559063
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	12.0
17	31.0
18	132.0
19	212.0
20	290.0
21	340.0
22	511.0
23	671.0
24	1059.0
25	1375.0
26	1717.0
27	2213.0
28	3115.0
29	3653.0
30	4506.0
31	5761.0
32	7572.0
33	9227.0
34	11992.0
35	16291.0
36	29416.0
37	38572.0
38	74088.0
39	474928.0
40	457460.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.358704232830107	15.792162383071473	25.66995941121815	30.179173972880267
2	32.48640994574461	15.244271561735928	27.096423651542533	25.172894840976927
3	29.158454446300592	20.175558860334476	21.01580326649161	29.650183426873326
4	27.02638270820089	19.76843086982947	21.34133349168314	31.863852930286495
5	26.51718910460169	20.159648044263427	20.52929587894623	32.79386697218865
6	28.853052541863732	22.484246522708062	22.916506570352723	25.746194365075485
7	27.920855368407814	22.242792172862103	22.517430122325226	27.318922336404857
8	24.809805579036347	25.55119705469356	22.83346024604766	26.805537120222432
9	25.216653975395236	25.22791893421264	23.282312093500902	26.273114996891223
10	27.481521974528967	22.293615475433658	22.89214282221275	27.33271972782462
11	25.519934610843702	24.2608789811587	22.92969268493744	27.289493723060154
12	27.885575962499036	22.265584066283367	22.606589214980826	27.242250756236768
13	25.822778619981417	21.15698986328357	22.512277931858353	30.507953584876663
14	25.88512885715683	23.3609048294363	22.731377014593797	28.022589298813077
15	26.07785571072284	21.89069671587154	22.43176404015565	29.599683533249966
16	26.130512843799558	23.517915650782783	22.165160014810365	28.186411490607295
17	26.309267655421504	21.555542359738165	22.30016487009494	29.835025114745395
18	28.60845448258036	22.01618311758172	22.103333729207854	27.27202867063007
19	26.337997666668993	21.598855689764783	24.075050823302572	27.988095820263652
20	26.257309124441992	22.01915217649483	21.943441174210403	29.78009752485277
21	26.651408032526913	22.031814339506646	23.862239159441955	27.454538468524483
22	28.655697449403743	21.570213003779436	21.82598869661807	27.948100850198752
23	26.46121361156326	23.94292770166896	21.985793926353367	27.61006476041441
24	26.85077160601636	24.4303773149927	21.702336125413048	27.016514953577893
25	26.60084670574181	22.07809672844638	21.646360632374616	29.67469593343719
26	26.708518753973298	22.440496566370694	23.90686236840083	26.94412231125518
27	26.89443423709158	22.96811580028363	21.549429591387632	28.588020371237157
28	26.611325737199863	22.80953312421844	21.600078243434886	28.97906289514681
29	26.761874489147214	25.236127508854782	21.5930048972007	26.4089931047973
30	29.04909775539146	23.62471444639277	21.375914295494717	25.950273502721053
31	26.877667786758696	23.468664202929936	23.522631214938908	26.13103679537246
32	27.059129681507304	23.835517629223922	21.320637404553487	27.784715284715283
33	27.391053002941113	26.338434292979745	21.061892652801745	25.208620051277393
34	27.298400899799503	24.429678712895498	20.87685042230497	27.395069965000037
35	27.67582068281369	24.78369532565337	22.715221841095968	24.825262150436973
36	30.042946563925582	25.46806340512634	20.133013839307544	24.355976191640526
37	28.005211571645138	27.635301761175885	19.910421746086083	24.44906492109289
38	30.139266328077518	25.92136884094926	19.63613309767156	24.303231733301665
39	28.566794819929196	26.355993364982716	19.169379449286357	25.90783236580173
40	28.118734412440705	26.712885017080822	20.94112181524769	24.227258755230782
41	30.324570534360745	27.041489978552917	18.55356182279259	24.08037766429375
42	28.350146357139366	27.512959068903125	20.33176613596194	23.80512843799557
43	28.074111203481834	29.60797943315426	18.222511753980285	24.095397609383625
44	27.95334036592778	27.87230252265217	20.009361268102527	24.16499584331752
45	28.268147935980103	28.04040365229176	17.621713950385278	26.069734461342854
46	28.006434125315245	28.08380430758053	19.63953878289543	24.270222784208798
47	29.85607050292365	28.4468154223399	17.447412727133006	24.249701347603445
48	29.94627749872505	28.606271351026596	17.168583165086662	24.278867985161693
49	29.77555661122095	28.537284393927752	17.195566671091147	24.491592323760155
50	29.530783901413272	28.680235848068016	17.195479345828996	24.593500904689716
51	29.365651830686794	28.77533305854984	17.137582697023255	24.721432413740104
52	29.083678559203037	28.86064983967082	17.023273928868335	25.03239767225781
53	28.835936790482247	28.787383944726603	17.08064662610117	25.29603263868998
54	28.527067338256153	28.981944628797773	17.067897137827206	25.423090895118865
55	28.134889585938538	28.843883389337932	17.199147006839315	25.822080017884215
56	27.84531901664769	28.715427928714643	17.311272643440475	26.127980411197193
57	27.641327204264265	28.781533152162524	17.287520172135558	26.289619471437653
58	27.137722417442696	28.653863618898583	17.536921120837203	26.671492842821515
59	26.493960584869676	28.35721970337355	18.06724743787681	27.081572273879967
60	26.595432539488485	28.35608447496559	17.88587286839035	27.162610117155573
61	26.81784998218565	27.935002060876187	18.096763376483658	27.150384580454514
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	5.5
2	3.0
3	3.0
4	5.0
5	7.0
6	4.5
7	5.5
8	9.0
9	11.0
10	13.0
11	17.5
12	19.0
13	16.0
14	18.5
15	21.0
16	36.5
17	72.5
18	93.0
19	109.5
20	168.0
21	210.0
22	246.5
23	283.0
24	402.5
25	680.5
26	839.0
27	1095.5
28	1352.0
29	1745.0
30	2751.0
31	3364.0
32	4158.0
33	4952.0
34	5912.5
35	8085.0
36	9297.0
37	11277.5
38	13258.0
39	15768.0
40	21301.5
41	24325.0
42	27840.0
43	34829.0
44	38303.0
45	42904.0
46	47505.0
47	51006.5
48	58784.0
49	63060.0
50	67325.5
51	71591.0
52	75647.0
53	83372.0
54	87041.0
55	89535.0
56	92029.0
57	92760.0
58	92091.0
59	90691.0
60	87041.0
61	79864.0
62	76337.0
63	62507.5
64	48678.0
65	41820.5
66	29648.0
67	24333.0
68	20228.5
69	16124.0
70	13292.0
71	8444.5
72	6429.0
73	5188.5
74	3948.0
75	3102.0
76	1755.0
77	1254.0
78	997.0
79	740.0
80	561.5
81	284.5
82	186.0
83	157.5
84	99.5
85	70.0
86	53.0
87	36.0
88	29.5
89	18.5
90	14.0
91	11.5
92	9.0
93	9.5
94	8.5
95	7.0
96	9.0
97	11.0
98	8.5
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.032572322782112986
3	8.732526215043697E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.080398622356664
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1145144.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.41605184004723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.27735939382859	59.73924138233251
2	14.637263017039793	21.784946469448784
3	3.213399188927972	7.173854418780891
4	0.82879309881743	2.4670204082508507
5	0.30656682243284383	1.1406746275300532
6	0.16994254535605594	0.7587871959027502
7	0.11090838026799135	0.5777354642862962
8	0.07949699393417589	0.47326819373868434
9	0.0659261852178351	0.4415369775108275
>10	0.3078998761871075	3.453032158874719
>50	9.006045227095337E-4	0.03982869152188972
>100	9.006045227095337E-4	0.12839008492993778
>500	2.5731557791700964E-4	0.14658107384618546
>1k	2.5731557791700964E-4	0.40786111991165913
>5k	0.0	0.0
>10k+	1.2865778895850482E-4	1.2672417331339718
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGCTGTATCTGGGGGGGGGGGGGGG	13236	1.1558371698231837	TruSeq Adapter, Index 4 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGCTGTATCTGGGGGGGGGGGGTGG	2642	0.2307133426014545	TruSeq Adapter, Index 4 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGCTGTATCTGGGGGGGGGGGGGGT	1618	0.14129227415940704	TruSeq Adapter, Index 4 (97% over 38bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.068124183508799	0.0	0.0	0.0	0.0
2	2.0738003255485773	0.0	0.0	0.0	0.0
3	2.077293336034595	0.0	0.0	0.0	0.0
4	2.0806116959963115	0.0	0.0	0.0	0.0
5	2.0840173812201783	0.0	0.0	0.0	0.0
6	2.0917893295515673	0.0	0.0	0.0	0.0
7	2.10436416730123	0.0	0.0	0.0	0.0
8	2.215267250232285	0.0	0.0	0.0	3.493010486017479E-4
9	2.230025219535709	0.0	0.0	0.0	3.493010486017479E-4
10	2.2446958635769825	0.0	0.0	0.0	3.493010486017479E-4
11	2.2659159022795388	0.0	0.0	0.0	4.3662631075218485E-4
12	2.3067841249659433	0.0	0.0	0.0	4.3662631075218485E-4
13	2.3662526284903906	8.732526215043697E-5	0.0	0.0	4.3662631075218485E-4
14	2.444583388639333	8.732526215043697E-5	0.0	0.0	4.3662631075218485E-4
15	2.542736983296424	2.6197578645131093E-4	0.0	0.0	4.3662631075218485E-4
16	2.6544259935868326	2.6197578645131093E-4	0.0	0.0	4.3662631075218485E-4
17	2.7629712944398257	2.6197578645131093E-4	0.0	0.0	4.3662631075218485E-4
18	2.876319484711093	2.6197578645131093E-4	0.0	0.0	4.3662631075218485E-4
19	3.0264316103476943	2.6197578645131093E-4	0.0	0.0	4.3662631075218485E-4
20	3.198287726259754	2.6197578645131093E-4	0.0	0.0	4.3662631075218485E-4
21	3.424285504705085	2.6197578645131093E-4	0.0	0.0	6.112768350530588E-4
22	3.7142927003066863	2.6197578645131093E-4	0.0	0.0	6.112768350530588E-4
23	4.083067282367982	2.6197578645131093E-4	0.0	0.0	6.112768350530588E-4
24	4.574184556702039	2.6197578645131093E-4	0.0	0.0	6.112768350530588E-4
25	5.189478353814018	2.6197578645131093E-4	0.0	0.0	6.986020972034958E-4
26	5.959861816505173	2.6197578645131093E-4	0.0	0.0	6.986020972034958E-4
27	6.85843876403317	2.6197578645131093E-4	0.0	0.0	6.986020972034958E-4
28	7.891409290010689	2.6197578645131093E-4	0.0	0.0	6.986020972034958E-4
29	9.094314776132958	2.6197578645131093E-4	0.0	0.0	6.986020972034958E-4
30	10.443315425832909	2.6197578645131093E-4	0.0	0.0	6.986020972034958E-4
31	11.917365850932285	2.6197578645131093E-4	0.0	0.0	6.986020972034958E-4
32	13.55724694885534	4.3662631075218485E-4	0.0	0.0	6.986020972034958E-4
33	15.294146413027532	4.3662631075218485E-4	0.0	0.0	6.986020972034958E-4
34	17.146839174811202	4.3662631075218485E-4	0.0	1.7465052430087395E-4	6.986020972034958E-4
35	19.09253334078509	4.3662631075218485E-4	0.0	1.7465052430087395E-4	6.986020972034958E-4
36	21.108786318576527	4.3662631075218485E-4	0.0	1.7465052430087395E-4	6.986020972034958E-4
37	23.176299225250276	4.3662631075218485E-4	0.0	1.7465052430087395E-4	6.986020972034958E-4
38	25.309131428012545	4.3662631075218485E-4	0.0	1.7465052430087395E-4	6.986020972034958E-4
39	27.511387214184417	4.3662631075218485E-4	0.0	1.7465052430087395E-4	6.986020972034958E-4
40	29.882879358403834	4.3662631075218485E-4	0.0	1.7465052430087395E-4	6.986020972034958E-4
41	32.259698343614424	4.3662631075218485E-4	0.0	1.7465052430087395E-4	6.986020972034958E-4
42	34.6423681213891	4.3662631075218485E-4	0.0	1.7465052430087395E-4	6.986020972034958E-4
43	37.07603585225963	4.3662631075218485E-4	0.0	1.7465052430087395E-4	6.986020972034958E-4
44	39.523675625074226	4.3662631075218485E-4	0.0	1.7465052430087395E-4	6.986020972034958E-4
45	41.95629545279895	4.3662631075218485E-4	0.0	1.7465052430087395E-4	7.859273593539327E-4
46	44.30246327099474	4.3662631075218485E-4	0.0	1.7465052430087395E-4	7.859273593539327E-4
47	46.642081694529246	4.3662631075218485E-4	0.0	1.7465052430087395E-4	7.859273593539327E-4
48	48.92223161453931	4.3662631075218485E-4	0.0	1.7465052430087395E-4	7.859273593539327E-4
49	51.11584219975828	4.3662631075218485E-4	0.0	1.7465052430087395E-4	7.859273593539327E-4
50	53.277928365340955	4.3662631075218485E-4	0.0	1.7465052430087395E-4	7.859273593539327E-4
>>END_MODULE