FastQCFastQC Report
Fri 9 Aug 2024
AM-AL-31A_S32_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAM-AL-31A_S32_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145144
Sequences flagged as poor quality0
Sequence length61
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGCTGTATCTGGGGGGGGGGGGGGG132361.1558371698231837TruSeq Adapter, Index 4 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGCTGTATCTGGGGGGGGGGGGTGG26420.2307133426014545TruSeq Adapter, Index 4 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGCTGTATCTGGGGGGGGGGGGGGT16180.14129227415940704TruSeq Adapter, Index 4 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)