##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-31A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 653263 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.557727898258435 40.0 40.0 40.0 40.0 40.0 2 39.310669669030695 40.0 40.0 40.0 40.0 40.0 3 39.290252164901425 40.0 40.0 40.0 40.0 40.0 4 39.16643067187335 40.0 40.0 40.0 40.0 40.0 5 39.208343347166455 40.0 40.0 40.0 40.0 40.0 6 39.34368240662643 40.0 40.0 40.0 40.0 40.0 7 39.368468748421385 40.0 40.0 40.0 40.0 40.0 8 39.356528687527074 40.0 40.0 40.0 40.0 40.0 9 39.378461660923705 40.0 40.0 40.0 40.0 40.0 10-11 39.376661467127335 40.0 40.0 40.0 40.0 40.0 12-13 39.29104816896105 40.0 40.0 40.0 40.0 40.0 14-15 39.32431501554504 40.0 40.0 40.0 40.0 40.0 16-17 39.43286241529063 40.0 40.0 40.0 40.0 40.0 18-19 39.47025317521427 40.0 40.0 40.0 40.0 40.0 20-21 39.44708639552523 40.0 40.0 40.0 40.0 40.0 22-23 39.491524852930596 40.0 40.0 40.0 40.0 40.0 24-25 39.45876010121498 40.0 40.0 40.0 40.0 40.0 26-27 39.432041918798404 40.0 40.0 40.0 40.0 40.0 28-29 39.446673085725045 40.0 40.0 40.0 40.0 40.0 30-31 39.427056164835285 40.0 40.0 40.0 40.0 40.0 32-33 39.42243062025976 40.0 40.0 40.0 40.0 40.0 34-35 39.42615015478705 40.0 40.0 40.0 40.0 40.0 36-37 39.4045023439124 40.0 40.0 40.0 40.0 40.0 38-39 39.3464822170805 40.0 40.0 40.0 40.0 40.0 40-41 39.39647262246277 40.0 40.0 40.0 40.0 40.0 42-43 39.37741972362681 40.0 40.0 40.0 40.0 40.0 44-45 39.27455659432928 40.0 40.0 40.0 40.0 40.0 46-47 39.256661416202746 40.0 40.0 40.0 40.0 40.0 48-49 39.35079311183852 40.0 40.0 40.0 40.0 40.0 50-51 39.305693967175905 40.0 40.0 40.0 40.0 40.0 52-53 39.26054433795562 40.0 40.0 40.0 40.0 40.0 54-55 39.17146175297033 40.0 40.0 40.0 40.0 40.0 56-57 39.1889777072047 40.0 40.0 40.0 40.0 40.0 58-59 39.13405181836819 40.0 40.0 40.0 40.0 40.0 60-61 39.049453139348614 40.0 40.0 40.0 40.0 40.0 62-63 39.376958953974714 40.0 40.0 40.0 40.0 40.0 64-65 39.37952458059563 40.0 40.0 40.0 40.0 40.0 66-67 39.37300154027187 40.0 40.0 40.0 40.0 40.0 68-69 39.365028383431344 40.0 40.0 40.0 40.0 40.0 70-71 39.37800813638414 40.0 40.0 40.0 40.0 40.0 72-73 39.38281469788572 40.0 40.0 40.0 40.0 40.0 74-75 39.36113322023844 40.0 40.0 40.0 40.0 40.0 76-77 39.374141647956776 40.0 40.0 40.0 40.0 40.0 78-79 39.36411007401573 40.0 40.0 40.0 40.0 40.0 80-81 39.372938709354315 40.0 40.0 40.0 40.0 40.0 82-83 39.369179474048664 40.0 40.0 40.0 40.0 40.0 84-85 39.352484067297766 40.0 40.0 40.0 40.0 40.0 86-87 39.34797110204464 40.0 40.0 40.0 40.0 40.0 88-89 39.38731607497931 40.0 40.0 40.0 40.0 40.0 90-91 39.47642381749988 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.020175183684543185 1101 2 0.13735099311128351 1101 3 -0.03909025332995242 1101 4 0.007723919567851567 1101 5 0.024613921349981638 1101 6 0.11360393440217109 1101 7 0.18044786352350428 1101 8 0.05848229947647354 1101 9 -0.08055413580817117 1101 10-11 0.006829349411098917 1101 12-13 -0.06424683925841634 1101 14-15 -0.00842304763514079 1101 16-17 0.036102182150585804 1101 18-19 -0.0406640118833792 1101 20-21 0.03679446720440893 1101 22-23 -0.021092641080791452 1101 24-25 0.0552220392200482 1101 26-27 0.08800423253409662 1101 28-29 0.029783833861515063 1101 30-31 0.06368875223586201 1101 32-33 0.05104244783953504 1101 34-35 -0.013978639409472748 1101 36-37 -0.08677815687412505 1101 38-39 -0.01708352497551857 1101 40-41 -0.11574914265985825 1101 42-43 -0.1155088528026269 1101 44-45 -0.1319092509443891 1101 46-47 -0.07876664621007734 1101 48-49 -0.010505743879818397 1101 50-51 0.019955701769326595 1101 52-53 -0.08460921847570546 1101 54-55 0.034441057466828795 1101 56-57 -0.0569567977204386 1101 58-59 -0.1751862918252698 1101 60-61 0.007578708096694697 1101 62-63 -0.05154311094448616 1101 64-65 0.004934398678550167 1101 66-67 -0.10808462153587328 1101 68-69 -0.3174772734792697 1101 70-71 0.04944989200674854 1101 72-73 0.043237498087847825 1101 74-75 -0.09412660116521465 1101 76-77 -0.0721290342437797 1101 78-79 0.07734318749261604 1101 80-81 0.06009468844237631 1101 82-83 -0.6686657756063852 1101 84-85 0.1512283212941412 1101 86-87 0.6716924880640462 1101 88-89 0.18531766637264724 1101 90-91 0.5390165755297289 1102 1 0.06023974345963268 1102 2 0.023544718311185875 1102 3 -0.0038843138245141517 1102 4 -0.015719491293296528 1102 5 -0.040667840471066086 1102 6 -0.12866959201588912 1102 7 -0.02880240454893368 1102 8 -0.18619571422924253 1102 9 -0.20879754013760987 1102 10-11 -0.1234783487583897 1102 12-13 -0.013983257375215885 1102 14-15 -0.2277607318158772 1102 16-17 -0.09393676319530186 1102 18-19 -0.062204380598650744 1102 20-21 -0.07802694484333728 1102 22-23 -0.10095917638742691 1102 24-25 -0.03966811911958956 1102 26-27 -0.014995457209479923 1102 28-29 -0.02082266301687241 1102 30-31 -0.06397632323265157 1102 32-33 0.0355715006539441 1102 34-35 -0.1421434579177543 1102 36-37 -0.09049207616720167 1102 38-39 -0.03342808465255587 1102 40-41 -0.04002181070256228 1102 42-43 -0.07998209112637511 1102 44-45 -0.06433018183942352 1102 46-47 -0.06302464761053983 1102 48-49 -0.14019062555696848 1102 50-51 0.1481162855795759 1102 52-53 0.047949622150696314 1102 54-55 -0.15310937754868092 1102 56-57 -0.14080505759686446 1102 58-59 -0.0959437450941465 1102 60-61 -0.202824219553996 1102 62-63 -0.11734625504012541 1102 64-65 -0.22866144257164933 1102 66-67 -0.23188615725018735 1102 68-69 0.050588747701695524 1102 70-71 -0.16772991792542769 1102 72-73 -0.10131778672008096 1102 74-75 -0.22891662018027148 1102 76-77 0.08446011304304335 1102 78-79 -0.3647044146807801 1102 80-81 -0.438332544703087 1102 82-83 -0.3967987263994317 1102 84-85 -0.448278946505809 1102 86-87 -0.1283075119359509 1102 88-89 -1.0051585241035426 1102 90-91 0.5390165755297289 1103 1 0.03707796306446198 1103 2 0.11329528375308229 1103 3 0.10137322895703704 1103 4 0.10187108609756024 1103 5 0.02366451134048475 1103 6 -0.007739706811264568 1103 7 0.20764832732492522 1103 8 0.0814396717648691 1103 9 0.15053545179684136 1103 10-11 -0.024216623840537466 1103 12-13 -0.054177641363608586 1103 14-15 0.012434719044023268 1103 16-17 -0.024179369775765736 1103 18-19 -0.04629199208672219 1103 20-21 -0.024838065911922058 1103 22-23 -0.05926583793311124 1103 24-25 0.0067994693240578385 1103 26-27 0.010577452789235053 1103 28-29 0.06772233762325897 1103 30-31 -0.02538758086078019 1103 32-33 0.04255670992015581 1103 34-35 0.04848705373017026 1103 36-37 0.09083062198904912 1103 38-39 -0.07134937581835743 1103 40-41 -0.02973247504750276 1103 42-43 0.008341671633296244 1103 44-45 0.12250102833109366 1103 46-47 0.036283631450338305 1103 48-49 -0.07078388131762381 1103 50-51 -0.07172251050933909 1103 52-53 -0.014120644731896448 1103 54-55 0.03947467321548004 1103 56-57 -0.05745370976606523 1103 58-59 -0.08877903132091092 1103 60-61 0.11538652329782195 1103 62-63 0.12501171868103 1103 64-65 0.14789605646291903 1103 66-67 -0.024091185892707756 1103 68-69 0.205428485100974 1103 70-71 0.10534653896068846 1103 72-73 -0.017344021133524734 1103 74-75 -0.507513108919035 1103 76-77 0.4395511095118394 1103 78-79 0.048620465833217 1103 80-81 0.4176552191301255 1103 82-83 -0.8478734378515043 1103 84-85 0.24819801826384236 1103 86-87 -0.014021797650237033 1103 88-89 -0.18420614315116524 1103 90-91 0.5390165755297289 1104 1 0.06404851088075247 1104 2 -0.04749796310329657 1104 3 0.00848527513996089 1104 4 -0.02126575323939761 1104 5 1.2700060821657644E-4 1104 6 0.08985497565536349 1104 7 -0.11082613051820545 1104 8 0.008340347027662176 1104 9 -0.040819946824790065 1104 10-11 0.04485149287499013 1104 12-13 -0.08307999769299812 1104 14-15 0.020863256954392284 1104 16-17 -0.059680943841954104 1104 18-19 0.02882938246884237 1104 20-21 -0.013006786212862664 1104 22-23 0.1367279637783767 1104 24-25 -0.03485076053343761 1104 26-27 0.09095344963337482 1104 28-29 0.1023382577364913 1104 30-31 0.09625576487428589 1104 32-33 0.027222753874049488 1104 34-35 -0.08495820726419367 1104 36-37 -0.15331766987891626 1104 38-39 -0.008999098071299727 1104 40-41 -0.0864747266808692 1104 42-43 0.007088588695218334 1104 44-45 -0.048748333571239755 1104 46-47 0.08954486659089866 1104 48-49 0.08773733663916516 1104 50-51 0.054127237157906904 1104 52-53 -0.0847404844995836 1104 54-55 -0.0263826496278341 1104 56-57 0.03652350336096788 1104 58-59 -0.017078185615602592 1104 60-61 0.12904808178843297 1104 62-63 -0.18898721004622843 1104 64-65 -0.26621892871791175 1104 66-67 0.15055381538065404 1104 68-69 -0.15911875554428434 1104 70-71 -0.257209632296167 1104 72-73 0.04356702618841979 1104 74-75 -0.25996399938743764 1104 76-77 -0.030462367577108296 1104 78-79 0.005714914099421264 1104 80-81 -0.504913631199301 1104 82-83 -1.0307425603432705 1104 84-85 -0.3155446717946404 1104 86-87 0.12169248806404909 1104 88-89 0.08088416883570204 1104 90-91 -0.7943167578035997 1201 1 0.0863787621512202 1201 2 0.10417305609489347 1201 3 -0.012353939992735263 1201 4 0.07581922699775845 1201 5 -0.10014297398241467 1201 6 -0.08640490576755866 1201 7 -0.0419032056170181 1201 8 -0.13608723625861785 1201 9 0.05855893516279309 1201 10-11 0.08512270264348132 1201 12-13 -0.03677406178108811 1201 14-15 -0.05385176986661122 1201 16-17 0.02349064000897272 1201 18-19 0.07940109336664136 1201 20-21 0.004770952352920688 1201 22-23 0.011816190768783486 1201 24-25 0.02775511185505053 1201 26-27 0.06737478644873107 1201 28-29 0.04517157930622773 1201 30-31 0.06839809265520103 1201 32-33 0.018913883134210607 1201 34-35 0.022505099424556363 1201 36-37 1.1987902330901079E-4 1201 38-39 0.17088440716838704 1201 40-41 0.12743794339348113 1201 42-43 0.0526078908959704 1201 44-45 0.11558166463406394 1201 46-47 -0.0018131350805674629 1201 48-49 0.08384745007218442 1201 50-51 0.06477946722719707 1201 52-53 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18-19 -0.00281718889964111 1202 20-21 0.07989608948950888 1202 22-23 0.015060940396551814 1202 24-25 -0.06656547430591075 1202 26-27 0.06798884001036498 1202 28-29 0.07143629700076559 1202 30-31 0.08361596840944685 1202 32-33 0.017733858403992997 1202 34-35 0.10244813488127136 1202 36-37 -0.24136640473122384 1202 38-39 -0.06538552950202359 1202 40-41 -0.136823775792017 1202 42-43 -0.007890223388450579 1202 44-45 -0.10771978466623011 1202 46-47 -0.27544842649130175 1202 48-49 -0.08235315398306398 1202 50-51 -0.19789622295642317 1202 52-53 -0.16400470050910343 1202 54-55 -0.16171872313015 1202 56-57 0.032182686555401574 1202 58-59 -0.29317910808295267 1202 60-61 -0.16658030162321324 1202 62-63 -0.04557803669289484 1202 64-65 -0.07880597258319 1202 66-67 0.10360005232050895 1202 68-69 0.12666087363156464 1202 70-71 -0.09038841313707735 1202 72-73 0.17650819533287887 1202 74-75 0.06724569545271208 1202 76-77 0.11101124889651004 1202 78-79 -0.1728720042155416 1202 80-81 0.337733191839682 1202 82-83 -0.02482070442140838 1202 84-85 0.5466107166765397 1202 86-87 -0.31497417860261834 1202 88-89 0.7567462378012166 1202 90-91 0.5390165755297289 1203 1 0.040886505514052374 1203 2 0.11391152006455485 1203 3 0.080289865316999 1203 4 0.06704868338208314 1203 5 -0.026008176258009996 1203 6 0.10832486667239039 1203 7 0.04809363382672416 1203 8 -0.020845453704886552 1203 9 0.08573971898074007 1203 10-11 0.005822370785075748 1203 12-13 -0.031134444208397838 1203 14-15 0.07111859991847069 1203 16-17 0.002808749996333404 1203 18-19 -0.00948955138683516 1203 20-21 -0.01804431313380661 1203 22-23 -0.037844049433495286 1203 24-25 0.0157449980750215 1203 26-27 0.006425434362768101 1203 28-29 -0.04542774117417281 1203 30-31 -0.031090292300547162 1203 32-33 6.528477685705525E-4 1203 34-35 -0.0035069582507532004 1203 36-37 0.07074068680869772 1203 38-39 0.12513579209907277 1203 40-41 0.02039396491881007 1203 42-43 -0.03132769020127313 1203 44-45 -0.025371255381237745 1203 46-47 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12-13 -0.28969814468786836 1204 14-15 -0.20513700912518118 1204 16-17 -0.17254773055097417 1204 18-19 -0.10584979852106358 1204 20-21 -0.16822375448747096 1204 22-23 -0.08825457858130648 1204 24-25 -0.03744963453862482 1204 26-27 -0.0692233890592604 1204 28-29 -0.008241102949085644 1204 30-31 -0.08937029768138416 1204 32-33 -0.1449054307277109 1204 34-35 -0.05721103139316597 1204 36-37 -0.1518997018883752 1204 38-39 -0.005135897742164275 1204 40-41 -0.11925798805823007 1204 42-43 -0.030234261744759294 1204 44-45 0.07366316711569709 1204 46-47 -0.04804365658389287 1204 48-49 0.015738985530738603 1204 50-51 -0.008888973369032271 1204 52-53 -0.007701938177682166 1204 54-55 0.027634936355092066 1204 56-57 0.04902229898154786 1204 58-59 0.04915527692331523 1204 60-61 0.19717492042594387 1204 62-63 -0.10342570755297942 1204 64-65 -0.08943356023868176 1204 66-67 0.10676688626411845 1204 68-69 0.1830538367630794 1204 70-71 -0.0016864320126899202 1204 72-73 -0.002889975809615919 1204 74-75 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0.0658016895906357 2401 42-43 -0.02597241636676273 2401 44-45 0.018567020456885075 2401 46-47 0.056023157087089714 2401 48-49 0.12851291156472655 2401 50-51 0.041342755373619866 2401 52-53 0.06592179160247014 2401 54-55 -0.032414648580420646 2401 56-57 0.0978739433558502 2401 58-59 0.02533208622952543 2401 60-61 0.12651982738420742 2401 62-63 0.19751767971920486 2401 64-65 0.2998476944579309 2401 66-67 0.1668074506235513 2401 68-69 0.24666218608571455 2401 70-71 0.0352417632475408 2401 72-73 0.07764832281240075 2401 74-75 -0.06169844769276267 2401 76-77 0.0186447752800305 2401 78-79 -0.17916250560946168 2401 80-81 0.13603924983566174 2401 82-83 0.16963215535636778 2401 84-85 0.4017927895056701 2401 86-87 0.2506398564851011 2401 88-89 0.7567462378012166 2401 90-91 0.5390165755297289 2402 1 0.062329735361771554 2402 2 0.004745060220948005 2402 3 0.09693603489215263 2402 4 0.25375156569296564 2402 5 0.21116200269641183 2402 6 0.0016717700681141423 2402 7 0.09362495918594504 2402 8 0.10379384070125752 2402 9 0.11596490299152862 2402 10-11 0.10848825831531173 2402 12-13 0.06748681769627041 2402 14-15 0.07245677599836142 2402 16-17 0.05997202044311223 2402 18-19 0.03664186954316051 2402 20-21 0.1012482964299366 2402 22-23 0.03379004414437503 2402 24-25 0.03158965111236256 2402 26-27 0.07958523388639094 2402 28-29 -0.0026186086086212867 2402 30-31 0.12570110367268228 2402 32-33 0.11707334471372377 2402 34-35 0.10132937964549882 2402 36-37 0.1303413595687246 2402 38-39 -0.23549951379881406 2402 40-41 0.06593916305612879 2402 42-43 0.09767989047235659 2402 44-45 0.19689910526625454 2402 46-47 0.04886656820308133 2402 48-49 0.1269850736129854 2402 50-51 0.1303604265711229 2402 52-53 0.10990157070784079 2402 54-55 0.23130101565286765 2402 56-57 0.18022982190036885 2402 58-59 0.14412068020427427 2402 60-61 0.2702354166566394 2402 62-63 0.12782005305825805 2402 64-65 0.2551019752217272 2402 66-67 0.2491938884874756 2402 68-69 0.17518750182330223 2402 70-71 0.03438559392037632 2402 72-73 0.09304770128485274 2402 74-75 0.283171067733619 2402 76-77 0.4780547339735435 2402 78-79 0.3757272270834733 2402 80-81 0.25205986069142483 2402 82-83 0.27000784533708355 2402 84-85 0.05322122328704637 2402 86-87 0.517846334217893 2402 88-89 0.33250381355879455 2402 90-91 0.5390165755297289 2403 1 0.055431181506058635 2403 2 0.056232387892130475 2403 3 0.0854283180717843 2403 4 0.054102637136004716 2403 5 0.07708916887472839 2403 6 0.06322067026930966 2403 7 -0.1448409936354551 2403 8 -0.062152904461378 2403 9 -0.053883461150576295 2403 10-11 0.0806368155467041 2403 12-13 0.0763091755461005 2403 14-15 0.11780001584767064 2403 16-17 0.046114487935241755 2403 18-19 0.0525001717076492 2403 20-21 0.0734201459013093 2403 22-23 0.0066606608148660484 2403 24-25 0.11899935148638008 2403 26-27 -0.05861167668490452 2403 28-29 0.07599675211728396 2403 30-31 0.0334686571713263 2403 32-33 0.11822978220673974 2403 34-35 0.07652091834916774 2403 36-37 0.0771384782510367 2403 38-39 0.16235534330597545 2403 40-41 0.12404028247861021 2403 42-43 0.07088294182069177 2403 44-45 -0.006789734097985445 2403 46-47 0.05156103172081572 2403 48-49 0.05620826283664826 2403 50-51 0.13532764825073684 2403 52-53 0.1379548941767581 2403 54-55 0.11772416695247756 2403 56-57 0.17073800069073997 2403 58-59 -0.06116062669696021 2403 60-61 0.24791001503711385 2403 62-63 0.12832156032281006 2403 64-65 0.05666197916918492 2403 66-67 0.1590344238287713 2403 68-69 0.2638005733095099 2403 70-71 0.05028207965391118 2403 72-73 0.22756545075041146 2403 74-75 0.3517800774664934 2403 76-77 0.11760773002715297 2403 78-79 0.18948416303720705 2403 80-81 0.21420303139826302 2403 82-83 0.38942045981975326 2403 84-85 0.2132773833432111 2403 86-87 0.10169248806404596 2403 88-89 0.35674623780121806 2403 90-91 0.5390165755297289 2404 1 -0.016756103673564837 2404 2 -0.06175778447337876 2404 3 -0.007100388295810944 2404 4 0.15755758532451125 2404 5 0.07937804022437689 2404 6 0.04453925366416911 2404 7 0.04526135634036166 2404 8 0.09321919625914177 2404 9 0.0632945310586095 2404 10-11 0.026710853787918154 2404 12-13 0.07514755424101338 2404 14-15 0.06927988956511655 2404 16-17 0.09577013882507401 2404 18-19 0.04778112493727349 2404 20-21 0.03467673372624347 2404 22-23 0.09900541728811874 2404 24-25 0.05074248359932909 2404 26-27 0.12652657216301577 2404 28-29 0.061789327925005466 2404 30-31 -0.03468127790517883 2404 32-33 0.006837435550465898 2404 34-35 0.11664123940614957 2404 36-37 0.07432762628619827 2404 38-39 0.1863527332215611 2404 40-41 0.07206290828243311 2404 42-43 0.13157932402503292 2404 44-45 0.14591352847200056 2404 46-47 0.05038017200781297 2404 48-49 0.0661338528551596 2404 50-51 0.06422766540541858 2404 52-53 0.05102910169118502 2404 54-55 0.16172434053884643 2404 56-57 0.16038652241459772 2404 58-59 0.05696378109394118 2404 60-61 0.17328982517146585 2404 62-63 0.25880976313684556 2404 64-65 0.1459783946998101 2404 66-67 0.20297012465672992 2404 68-69 0.10666824376618678 2404 70-71 0.05709731050866651 2404 72-73 0.0786981024616793 2404 74-75 -0.07203330381823037 2404 76-77 0.13339742606652294 2404 78-79 -0.07345478049874998 2404 80-81 -0.3533993145933749 2404 82-83 -0.04295257255327556 2404 84-85 -0.36152634400289685 2404 86-87 -0.01810343030329875 2404 88-89 -0.6227409416859686 2404 90-91 0.09457213108528606 >>END_MODULE >>Per sequence quality scores pass #Quality Count 29 1.0 30 23.0 31 452.0 32 2871.0 33 5313.0 34 6925.0 35 8712.0 36 12319.0 37 18619.0 38 47071.0 39 254783.0 40 296174.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.796671478409152 13.805312714787151 26.410649309696094 30.987366497107597 2 31.3542014323726 15.394790425945601 27.49907376504558 25.75193437663622 3 29.949805820932763 18.267680857480066 21.291118584704783 30.491394736882388 4 27.53929121961599 19.94051400431373 19.721612887918035 32.79858188815224 5 26.973822181877743 20.4955737581954 20.973941582486685 31.556662477440174 6 27.22624731539977 22.842561112446287 23.376189987799705 26.555001584354233 7 26.27701247430208 22.56426584698659 23.061921461953304 28.096800216758027 8 25.349055434028866 23.56983328307282 23.39486546766004 27.686245815238276 9 25.59658208103015 23.520236107050298 23.666272236449945 27.216909575469607 10-11 25.87946967760305 22.494845108325435 23.30210037917347 28.323584834898043 12-13 26.21279637756922 21.914604072173073 23.08924583207682 28.783353718180887 14-15 26.45718493164315 21.61533716129645 23.036969796238267 28.890508110822132 16-17 26.627177721683303 21.519357441030643 22.72637513528242 29.127089702003634 18-19 26.91970921359391 21.73910048479709 22.50380015399617 28.83739014761283 20-21 26.92039806326089 21.813572787682755 22.495074724881096 28.770954424175255 22-23 26.93011849745049 21.44672207059025 22.511377500332944 29.11178193162631 24-25 27.01454085108142 21.750504773728192 22.462392635125518 28.772561740064873 26-27 27.192187526310228 21.881921982417495 22.51459213211218 28.411298359160092 28-29 27.227395398178068 21.780048770556423 22.410422754694512 28.582133076570997 30-31 27.30807530717825 21.814114010910156 22.54163640107188 28.336174280839714 32-33 27.214651103547904 21.85126114109496 22.701726404929058 28.232361350428082 34-35 27.10736623521836 21.667423739473776 22.83165196614304 28.393558059164825 36-37 27.30429509924716 21.783170374444097 22.840860817773358 28.07167370853538 38-39 27.441499494367488 21.72554737659039 22.918463817878223 27.914489311163898 40-41 27.141611309965825 21.777997203668818 22.97443036386703 28.105961122498325 42-43 27.114148148066885 21.902099804620864 23.147984611013413 27.83576743629883 44-45 27.13458243559354 21.96192264390514 23.202392214272955 27.701102706228365 46-47 26.966912297433655 21.894553530733702 23.35859018302434 27.7799439888083 48-49 27.105560738210592 22.0812637075566 23.284836295208557 27.52833925902425 50-51 27.078887808571324 22.038046951212607 23.465090733194096 27.417974507021974 52-53 27.01450428617933 21.957536079864 23.450103085325715 27.57785654863095 54-55 27.095709839169764 22.220619428032734 23.544482279417142 27.13918845338036 56-57 27.178376592689112 22.271265296009616 23.58372830341043 26.966629807890836 58-59 27.117881325290234 22.08028010373827 23.655603505458743 27.146235065512748 60-61 27.31467641443815 22.163354101611638 23.544123958502908 26.977845525447307 62-63 27.25755005089922 22.226331862911437 23.572446555819475 26.943671530369866 64-65 27.13918242219464 22.254153077564574 23.563013628906905 27.04365087133388 66-67 27.166412435534248 22.328303188784776 23.58810924674947 26.917175128931504 68-69 27.305838073287802 22.485885860486754 23.28251538282621 26.925760683399236 70-71 27.132192189207988 22.275290668786642 23.71248633608268 26.88003080592269 72-73 27.330057390394657 22.406565637961645 23.36503679910415 26.89834017253954 74-75 27.3797451611481 22.298840439134075 23.371985362197435 26.94942903752039 76-77 27.23937666449191 22.643507278308395 23.408237697466227 26.70887835973347 78-79 27.671103161660266 22.449892156346994 22.970698090378242 26.9083065916145 80-81 27.480903436556513 22.336792437265935 23.310180984277302 26.87212314190025 82-83 27.793690150478795 21.680489056087552 23.41185020519836 27.113970588235293 84-85 28.37672904800651 21.849936068813207 22.361385563175638 27.41194932000465 86-87 28.100610790634544 21.29284017645063 23.150661689854086 27.45588734306074 88-89 27.916873537130293 21.001489467338107 22.51932761188737 28.56230938364423 90-91 25.57535905866067 19.224779373594046 24.381380861740787 30.818480706004497 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 3.5 10 6.5 11 7.0 12 9.0 13 20.0 14 34.0 15 45.5 16 60.0 17 89.0 18 138.0 19 199.5 20 274.0 21 366.0 22 485.5 23 634.5 24 830.5 25 1076.0 26 1366.0 27 1763.5 28 2240.5 29 2729.5 30 3353.0 31 4087.0 32 4883.0 33 5763.5 34 6820.5 35 8159.0 36 9673.5 37 11268.0 38 12860.0 39 14633.0 40 16678.5 41 18773.5 42 20805.5 43 22918.0 44 25198.5 45 27382.5 46 29509.5 47 32002.5 48 34270.0 49 36258.0 50 38519.0 51 40626.0 52 42246.0 53 43887.5 54 45718.5 55 47344.5 56 48683.5 57 49554.0 58 50161.5 59 50639.0 60 50558.5 61 49823.0 62 48706.0 63 47241.5 64 45102.5 65 42426.5 66 39112.0 67 35785.5 68 32532.5 69 28579.5 70 24655.5 71 21165.0 72 17863.5 73 14507.5 74 11398.0 75 9154.0 76 7303.0 77 5456.5 78 4119.0 79 3108.5 80 2222.0 81 1607.5 82 1188.5 83 836.5 84 580.0 85 423.5 86 305.0 87 220.0 88 161.5 89 113.0 90 73.5 91 47.5 92 34.5 93 23.5 94 15.5 95 9.5 96 3.5 97 1.5 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.012399294005630196 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.06289402779370759 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 5.286166630544528E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.001069541594472609 78-79 0.0 80-81 0.0016497838783119413 82-83 0.0021374372127818744 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 13952.0 32-33 30186.0 34-35 33157.0 36-37 35036.0 38-39 37179.0 40-41 38340.0 42-43 38733.0 44-45 37866.0 46-47 36443.0 48-49 34907.0 50-51 33197.0 52-53 30882.0 54-55 28738.0 56-57 26346.0 58-59 23797.0 60-61 22064.0 62-63 20043.0 64-65 18164.0 66-67 15919.0 68-69 14516.0 70-71 12941.0 72-73 11541.0 74-75 10237.0 76-77 8968.0 78-79 7969.0 80-81 7111.0 82-83 6397.0 84-85 5590.0 86-87 4877.0 88-89 4274.0 90-91 3893.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.93576665960421 #Duplication Level Percentage of deduplicated Percentage of total 1 99.93806665047926 99.87387309194268 2 0.05959207290628593 0.11910758985449428 3 0.002341276614454342 0.007019318202830919 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0026023209641446096 0.0 0.0 0.0 0.0 2 0.0026023209641446096 0.0 0.0 0.0 0.0 3 0.002755398667917822 0.0 0.0 0.0 0.0 4 0.002755398667917822 0.0 0.0 0.0 0.0 5 0.002908476371691034 0.0 0.0 0.0 0.0 6 0.0032146317792374588 0.0 0.0 0.0 0.0 7 0.0044392534094231575 0.0 0.0 0.0 0.0 8 0.01852240215655869 0.0 0.0 0.0 3.061554075464246E-4 9 0.019593946082971176 0.0 0.0 0.0 3.061554075464246E-4 10-11 0.01974702378674439 0.0 0.0 0.0 3.8269425943303077E-4 12-13 0.01974702378674439 7.653885188660616E-5 0.0 0.0 4.5923311131963697E-4 14-15 0.01974702378674439 2.2961655565981848E-4 0.0 0.0 4.5923311131963697E-4 16-17 0.01974702378674439 3.061554075464246E-4 0.0 0.0 4.5923311131963697E-4 18-19 0.01974702378674439 3.061554075464246E-4 0.0 0.0 4.5923311131963697E-4 20-21 0.019823562638630998 3.061554075464246E-4 0.0 0.0 5.357719632062431E-4 22-23 0.019900101490517602 3.061554075464246E-4 0.0 0.0 6.123108150928493E-4 24-25 0.02035933460183724 3.061554075464246E-4 0.0 0.0 6.888496669794554E-4 26-27 0.02074202886127027 3.061554075464246E-4 0.0 0.0 7.653885188660616E-4 28-29 0.021430878528249726 3.061554075464246E-4 0.0 0.0 7.653885188660616E-4 30-31 0.02166049508390954 3.061554075464246E-4 0.0 0.0 7.653885188660616E-4 32-33 0.022043189343342574 4.5923311131963697E-4 0.0 0.0 7.653885188660616E-4 34-35 0.022425883602775608 4.5923311131963697E-4 0.0 1.530777037732123E-4 7.653885188660616E-4 36-37 0.023191272121641668 4.5923311131963697E-4 0.0 1.530777037732123E-4 7.653885188660616E-4 38-39 0.023573966381074698 4.5923311131963697E-4 0.0 1.530777037732123E-4 7.653885188660616E-4 40-41 0.024033199492394335 4.5923311131963697E-4 0.0 1.530777037732123E-4 7.653885188660616E-4 42-43 0.024798588011260395 4.5923311131963697E-4 0.0 1.530777037732123E-4 7.653885188660616E-4 44-45 0.025028204566920214 4.5923311131963697E-4 0.0 1.530777037732123E-4 7.653885188660616E-4 46-47 0.02510474341880682 4.5923311131963697E-4 0.0 1.530777037732123E-4 7.653885188660616E-4 48-49 0.02533435997446664 4.5923311131963697E-4 0.0 1.530777037732123E-4 7.653885188660616E-4 50-51 0.02571705423389967 4.5923311131963697E-4 0.0 1.530777037732123E-4 7.653885188660616E-4 52-53 0.026176287345219307 4.5923311131963697E-4 0.0 1.530777037732123E-4 7.653885188660616E-4 54-55 0.026252826197105915 4.5923311131963697E-4 0.0 1.530777037732123E-4 7.653885188660616E-4 56-57 0.026405903900879123 5.357719632062431E-4 0.0 1.530777037732123E-4 7.653885188660616E-4 58-59 0.02648244275276573 6.123108150928493E-4 0.0 1.530777037732123E-4 7.653885188660616E-4 60-61 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1.530777037732123E-4 7.653885188660616E-4 >>END_MODULE