##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-30C_S31_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1455322
Sequences flagged as poor quality	0
Sequence length	61
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.444477579532226	40.0	40.0	40.0	40.0	40.0
2	39.21247943754028	40.0	40.0	40.0	40.0	40.0
3	39.164517543196624	40.0	40.0	40.0	40.0	40.0
4	39.05270311312548	40.0	40.0	40.0	40.0	40.0
5	39.07912063447127	40.0	40.0	40.0	40.0	40.0
6	39.19797543086685	40.0	40.0	40.0	40.0	40.0
7	39.20110326099653	40.0	40.0	40.0	40.0	40.0
8	39.18813568406167	40.0	40.0	40.0	40.0	40.0
9	39.222775440761566	40.0	40.0	40.0	40.0	40.0
10	39.22736823878152	40.0	40.0	40.0	40.0	40.0
11	39.21948544720687	40.0	40.0	40.0	40.0	40.0
12	39.223204211851396	40.0	40.0	40.0	40.0	40.0
13	39.06489148106055	40.0	40.0	40.0	40.0	40.0
14	39.21994445215561	40.0	40.0	40.0	40.0	40.0
15	39.13186497558616	40.0	40.0	40.0	40.0	40.0
16	39.311803161087376	40.0	40.0	40.0	40.0	40.0
17	39.2614198094992	40.0	40.0	40.0	40.0	40.0
18	39.343378303908004	40.0	40.0	40.0	40.0	40.0
19	39.29181858035541	40.0	40.0	40.0	40.0	40.0
20	39.30793047861573	40.0	40.0	40.0	40.0	40.0
21	39.28649467265664	40.0	40.0	40.0	40.0	40.0
22	39.34495596163598	40.0	40.0	40.0	40.0	40.0
23	39.3359765055431	40.0	40.0	40.0	40.0	40.0
24	39.33654820032955	40.0	40.0	40.0	40.0	40.0
25	39.292500216446946	40.0	40.0	40.0	40.0	40.0
26	39.26271986543184	40.0	40.0	40.0	40.0	40.0
27	39.27953538804471	40.0	40.0	40.0	40.0	40.0
28	39.29718646457622	40.0	40.0	40.0	40.0	40.0
29	39.297914825722415	40.0	40.0	40.0	40.0	40.0
30	39.28936414071937	40.0	40.0	40.0	40.0	40.0
31	39.23809576162526	40.0	40.0	40.0	40.0	40.0
32	39.26747757540943	40.0	40.0	40.0	40.0	40.0
33	39.26645924407107	40.0	40.0	40.0	40.0	40.0
34	39.261969516024635	40.0	40.0	40.0	40.0	40.0
35	39.26854675460139	40.0	40.0	40.0	40.0	40.0
36	39.23568804704389	40.0	40.0	40.0	40.0	40.0
37	39.255980463430085	40.0	40.0	40.0	40.0	40.0
38	39.24361481514057	40.0	40.0	40.0	40.0	40.0
39	38.0369801322319	40.0	40.0	40.0	40.0	40.0
40	39.238777397716795	40.0	40.0	40.0	40.0	40.0
41	39.2524073710148	40.0	40.0	40.0	40.0	40.0
42	39.214716743098776	40.0	40.0	40.0	40.0	40.0
43	39.24332072214946	40.0	40.0	40.0	40.0	40.0
44	39.213749259614026	40.0	40.0	40.0	40.0	40.0
45	39.11928494175172	40.0	40.0	40.0	40.0	40.0
46	39.09235207053834	40.0	40.0	40.0	40.0	40.0
47	39.121747626985645	40.0	40.0	40.0	40.0	40.0
48	39.26351693989371	40.0	40.0	40.0	40.0	40.0
49	39.240459499684604	40.0	40.0	40.0	40.0	40.0
50	39.24014616696511	40.0	40.0	40.0	40.0	40.0
51	39.20794710723813	40.0	40.0	40.0	40.0	40.0
52	39.22541678061625	40.0	40.0	40.0	40.0	40.0
53	39.15566726813722	40.0	40.0	40.0	40.0	40.0
54	39.0776749063094	40.0	40.0	40.0	40.0	40.0
55	39.12060973447801	40.0	40.0	40.0	40.0	40.0
56	39.12464458037465	40.0	40.0	40.0	40.0	40.0
57	39.054345361370196	40.0	40.0	40.0	40.0	40.0
58	39.023688228447035	40.0	40.0	40.0	40.0	40.0
59	38.98048129554834	40.0	40.0	40.0	40.0	40.0
60	38.948798959955255	40.0	40.0	40.0	40.0	40.0
61	38.84224659559877	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.008996170698971184
1101	2	0.06984525284953946
1101	3	0.005689771333052818
1101	4	0.03662359451351449
1101	5	0.06271392996193725
1101	6	0.03926838006076139
1101	7	0.04721542883309837
1101	8	-0.028618892396920614
1101	9	-0.03745074311684249
1101	10	0.003922703312198905
1101	11	0.11158547689276332
1101	12	0.14368790819885646
1101	13	0.01943131413865018
1101	14	0.03562668668004676
1101	15	0.07699495690790314
1101	16	0.03450719122426449
1101	17	0.08610012767090325
1101	18	0.10054431580518752
1101	19	-0.03293517200251728
1101	20	0.047748753580243886
1101	21	-0.026842245046196922
1101	22	0.10417451390843979
1101	23	-0.030113354366143597
1101	24	0.035647372720362114
1101	25	0.0846682068909459
1101	26	0.10958512448428337
1101	27	0.07131973050619678
1101	28	0.010916779086478812
1101	29	0.060761439219511715
1101	30	0.055904205795656026
1101	31	0.09359367560242049
1101	32	0.1436465573446455
1101	33	0.12961446393654796
1101	34	0.028600422687901528
1101	35	0.04135281588504114
1101	36	0.03842788204246261
1101	37	-0.06044178387475796
1101	38	0.008634685026017053
1101	39	1.2427647742172852
1101	40	0.011218725850170586
1101	41	0.031259013259855806
1101	42	2.361080774448965E-6
1101	43	0.004086700100188523
1101	44	0.04345533862653639
1101	45	0.0787395151392829
1101	46	0.04155883385589476
1101	47	0.0018451154295036076
1101	48	0.07715367606802204
1101	49	0.005771940601121628
1101	50	0.0941662902934155
1101	51	0.06812678952788787
1101	52	0.057944128241523174
1101	53	0.02951703149233964
1101	54	-0.02152236851218703
1101	55	0.07130753882548646
1101	56	0.03286886489858176
1101	57	0.08065835116899223
1101	58	0.06621535294765124
1101	59	0.04213823240375092
1101	60	0.06345950082327789
1101	61	0.08101113924769265
1102	1	0.10850845667021503
1102	2	0.14766281315591812
1102	3	0.12195922744322019
1102	4	-0.020778704809401916
1102	5	-0.013258656411586855
1102	6	0.013966365132446867
1102	7	0.04893250342357902
1102	8	-0.01672849487596295
1102	9	0.06144777681349467
1102	10	0.05947682414983291
1102	11	-0.03869936069203561
1102	12	0.025665373409196945
1102	13	-0.00885828616506501
1102	14	-0.08355759261517193
1102	15	-0.11445537262375893
1102	16	0.0016524232366919023
1102	17	0.011496639195136993
1102	18	0.0835995850978648
1102	19	0.02177652855354495
1102	20	-0.07637185597086926
1102	21	-0.016460437705021036
1102	22	0.04285427223994276
1102	23	-0.09698991293320347
1102	24	0.011523690568978395
1102	25	-0.01070285483193345
1102	26	-0.07624875566507683
1102	27	-0.043252255334202516
1102	28	-0.024660161934484393
1102	29	0.03921063565914551
1102	30	0.027412907192257308
1102	31	-0.041019581103157066
1102	32	-0.002920245414962608
1102	33	0.003850484500723894
1102	34	0.005157425172185981
1102	35	0.06929217745902605
1102	36	-0.06672818625823851
1102	37	0.03155356462314529
1102	38	0.005984143164475597
1102	39	1.2297148942277687
1102	40	-0.02968851687186458
1102	41	0.0712867005928075
1102	42	0.015542366807494545
1102	43	0.015364839604913527
1102	44	0.010357967170087079
1102	45	0.05334626471839954
1102	46	0.019504161552482913
1102	47	-0.01939409253331803
1102	48	-0.0626385143623196
1102	49	0.032651160831726145
1102	50	0.019554507681959876
1102	51	-0.023992782502247678
1102	52	-0.01243689115676716
1102	53	-0.012413028962484418
1102	54	-0.010714921207750194
1102	55	-0.07666775897639866
1102	56	-0.016059749549086177
1102	57	-0.08569411227413326
1102	58	0.045787462257571576
1102	59	-0.019345252935323742
1102	60	-0.04320251861402369
1102	61	-0.03724513936590057
1103	1	0.028056831135728544
1103	2	-0.022575121613158444
1103	3	-0.01091519804533192
1103	4	0.02535894191851895
1103	5	-0.03670363581354508
1103	6	0.03851257963206933
1103	7	0.02131917046028775
1103	8	-0.04790784003255055
1103	9	0.04516205481445468
1103	10	-0.037723673779389344
1103	11	-0.08205678121855442
1103	12	-0.07157748698986666
1103	13	-0.007076225659261581
1103	14	0.0314045055034029
1103	15	0.003014999769298754
1103	16	-0.032007963178060095
1103	17	-0.09094734908578062
1103	18	0.005671363291376963
1103	19	-0.054531707017908104
1103	20	-0.019611063457944056
1103	21	0.03075461523128098
1103	22	-0.12823034457142057
1103	23	0.027641986280805497
1103	24	0.0013780681605197742
1103	25	-0.11225931154607593
1103	26	-0.0456411147331508
1103	27	0.030914216974146314
1103	28	0.043441101578189034
1103	29	-0.0918940220045883
1103	30	0.0015935491794110135
1103	31	-0.0486796234173994
1103	32	-0.11144684136309735
1103	33	0.05716403103043177
1103	34	-0.014627739424682318
1103	35	-0.08441626199127228
1103	36	0.01696435733715873
1103	37	-0.0021219512451224887
1103	38	0.050580476815639486
1103	39	1.1977425765888654
1103	40	0.03749646911235516
1103	41	0.013273189328906199
1103	42	0.09406555437342945
1103	43	-0.06966968651720151
1103	44	0.04795995446163204
1103	45	-0.005673544322363
1103	46	-0.03260224370496445
1103	47	-0.015830917112552356
1103	48	-0.08160120404252069
1103	49	-0.008010380196729727
1103	50	0.08504310369089296
1103	51	0.11278768964733388
1103	52	-0.01746762356841458
1103	53	0.032961904190152325
1103	54	0.03894940113433165
1103	55	0.06346615654460663
1103	56	0.007539928515058136
1103	57	0.03784752069601183
1103	58	0.013654772411413774
1103	59	0.08937689119947834
1103	60	0.09246499311142031
1103	61	0.10526693712937885
1104	1	-0.010549475906515227
1104	2	6.447353718002091E-4
1104	3	-0.07738382147798006
1104	4	-0.09045352558575814
1104	5	-0.06508980195754077
1104	6	0.02948894033572458
1104	7	0.09103580987653004
1104	8	-0.014550540711283588
1104	9	-0.051175408422366786
1104	10	0.038181067618658915
1104	11	-0.06460212955582278
1104	12	-0.005432282236476738
1104	13	-0.12491420866622605
1104	14	0.013881998610358437
1104	15	0.029996120111704272
1104	16	-0.06600269600800601
1104	17	-0.033728426124376654
1104	18	-0.05753984030782533
1104	19	0.020563196957489538
1104	20	-0.03344246708902432
1104	21	-0.036995328841165076
1104	22	-0.0484698403443673
1104	23	0.0027094300926862047
1104	24	-0.040635013977542656
1104	25	-0.013198419636090364
1104	26	-0.027668144993221233
1104	27	0.035190940375990465
1104	28	-0.07918749756672128
1104	29	-0.046549211180312966
1104	30	-0.017638614494778437
1104	31	-0.06331459523788396
1104	32	0.018578899831979356
1104	33	-0.0652171177908798
1104	34	-0.07301679633056324
1104	35	-0.11281588854779301
1104	36	-0.0997163989702301
1104	37	-0.06872462563548254
1104	38	-0.06008402825628423
1104	39	1.179664873776055
1104	40	-0.192234973069894
1104	41	-0.061940830077972464
1104	42	-0.04943903827783913
1104	43	-0.08515229554896564
1104	44	-0.09459263668391316
1104	45	-0.06494896017420615
1104	46	-0.0321534403609931
1104	47	6.933065823560014E-4
1104	48	-0.018231056941878876
1104	49	-0.03385179081477219
1104	50	-0.0060879457331139974
1104	51	-0.06913052331879754
1104	52	-0.007303352601638835
1104	53	0.0038271178421638297
1104	54	-0.02407649130599765
1104	55	5.15863681528117E-4
1104	56	-0.05004740409353303
1104	57	0.06779896686752096
1104	58	-0.08865579498507259
1104	59	-0.04222574654352229
1104	60	-0.06525692979332831
1104	61	0.05000290860215273
1201	1	-0.0032688908674600725
1201	2	0.030359998125526033
1201	3	0.0285877679467319
1201	4	0.07803296515015745
1201	5	0.02819061112383281
1201	6	0.05792179279511345
1201	7	0.14237383025333372
1201	8	0.10370994718824988
1201	9	0.01326122121448492
1201	10	0.07297009450941516
1201	11	-0.003407104692840335
1201	12	-0.05116584467488394
1201	13	-0.06818584896021918
1201	14	0.07427025842994084
1201	15	0.04605538401868614
1201	16	0.06291493350199318
1201	17	-0.014089533568650836
1201	18	0.010115012277125857
1201	19	0.0535080053544732
1201	20	0.06748196192955191
1201	21	0.12998054866310582
1201	22	0.04586404365858243
1201	23	-0.045230492975584013
1201	24	-0.004553726968211436
1201	25	0.0062696198935583425
1201	26	0.046790450672226314
1201	27	0.0415202349131647
1201	28	0.11830331954340068
1201	29	0.045730202005820786
1201	30	0.11907639363638367
1201	31	0.1219712770395347
1201	32	0.04620498724797528
1201	33	0.049503877192336176
1201	34	0.1411726947722869
1201	35	0.08187868313121527
1201	36	0.03806091730799466
1201	37	-0.0027416656473064904
1201	38	0.10551563548728637
1201	39	1.3466203151963896
1201	40	0.11244071088072616
1201	41	0.07693745720881395
1201	42	0.09596627646639178
1201	43	0.10271883340107024
1201	44	0.11512724352012782
1201	45	0.12425971967502392
1201	46	0.21382428725326008
1201	47	0.1575640008513588
1201	48	0.13336031564309536
1201	49	0.20688896015423808
1201	50	0.06695061826193438
1201	51	0.14681849620023968
1201	52	0.15401504361131657
1201	53	0.07241724345779943
1201	54	-0.0031628289738065973
1201	55	0.17243156612403965
1201	56	0.13481649066689272
1201	57	0.140860912688332
1201	58	0.18018917412053526
1201	59	0.14467586512706276
1201	60	0.14690441806030208
1201	61	0.19030785547845852
1202	1	0.0679668181786397
1202	2	0.05494382042755319
1202	3	0.0708354062186558
1202	4	-0.029926862990507175
1202	5	0.047222672358202544
1202	6	0.03177426161941099
1202	7	0.10434240892075053
1202	8	-0.01021337905240216
1202	9	0.013913014314397287
1202	10	-0.024125524355874006
1202	11	-0.06219408577597818
1202	12	0.015555621168410028
1202	13	0.009832373004684314
1202	14	-0.03608418472545338
1202	15	-0.025749622079331402
1202	16	-0.025867907914587818
1202	17	-0.043458126654293494
1202	18	-0.03574907436899366
1202	19	0.03638152850799514
1202	20	-0.0067451578658577205
1202	21	-0.02258585879750541
1202	22	-0.012230267904470793
1202	23	0.0034183926960409394
1202	24	-0.026652262923484216
1202	25	-0.04031537816295838
1202	26	0.03534505302269508
1202	27	-0.030995277688290912
1202	28	-0.0359739519035287
1202	29	0.037158045090905034
1202	30	0.09038709233332298
1202	31	0.06254067814052178
1202	32	-0.04605234988364515
1202	33	0.10194832427929867
1202	34	0.061478539580129166
1202	35	-0.08538985785556719
1202	36	-0.21531120155469097
1202	37	-0.04872466551576338
1202	38	-0.2258219375978996
1202	39	1.1022157397128822
1202	40	-0.03501911688833559
1202	41	-0.18739548792239447
1202	42	-0.10404873632792544
1202	43	-0.08248702396697638
1202	44	-0.08738717656609651
1202	45	-0.0993186913321793
1202	46	-0.034763060825810044
1202	47	-0.15628961251270823
1202	48	-0.0781506329202486
1202	49	-0.0993482613194061
1202	50	-0.16194841623702416
1202	51	-0.0658280736363821
1202	52	-0.05635954047111369
1202	53	-0.062035707410977636
1202	54	-0.04979836514981173
1202	55	-0.10589523565281667
1202	56	-0.01309669376231426
1202	57	-0.02571049953252924
1202	58	-0.027429596034323822
1202	59	-0.1419220944706865
1202	60	-0.034368384479094516
1202	61	-0.023697297923312988
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2403	6	-0.00560305079486767
2403	7	-0.0155507337643499
2403	8	0.07423448499603325
2403	9	0.03859590710821692
2403	10	-0.03463864018327456
2403	11	0.07383852577665806
2403	12	0.014201457986395383
2403	13	0.027801414968642746
2403	14	0.031111479811393394
2403	15	0.05590351219481704
2403	16	0.03512525340841677
2403	17	0.025697098410340402
2403	18	0.03389341240526278
2403	19	0.012871007650268496
2403	20	-0.01128248385234798
2403	21	0.0029807573715885383
2403	22	0.10229707007719213
2403	23	0.03158762486219757
2403	24	0.03584951723527752
2403	25	0.033557817186171235
2403	26	-0.03330914695722953
2403	27	0.06662776831865358
2403	28	0.010074477972558782
2403	29	0.12397588586586039
2403	30	-0.032650790400808205
2403	31	-0.041291370451837395
2403	32	-0.014372310122780618
2403	33	0.002083950871281104
2403	34	-0.011155569695340262
2403	35	-0.02318322562920372
2403	36	-0.07362413673779145
2403	37	-0.03110563382654874
2403	38	-0.05171543392527411
2403	39	1.227532225655601
2403	40	0.010703770935378998
2403	41	-0.07635028383734976
2403	42	0.0746684979983101
2403	43	-0.06049372405443165
2403	44	-0.10115609523315783
2403	45	0.028230519902173512
2403	46	-0.05810345107484238
2403	47	-0.07616115434524318
2403	48	-0.05367801391245308
2403	49	0.0764467408550118
2403	50	0.0014782818432763634
2403	51	-0.011799562422574184
2403	52	0.10499825471266178
2403	53	0.05921599508985054
2403	54	0.001724638324489547
2403	55	0.010072286605137037
2403	56	-0.04167691560115827
2403	57	-0.0768655238812741
2403	58	0.03215836292405072
2403	59	-0.09988951159174775
2403	60	0.05409398846058622
2403	61	-0.05723787837454353
2404	1	-0.013544944339777487
2404	2	-0.006039545296559368
2404	3	-0.07884416814151507
2404	4	0.014560856764191499
2404	5	0.008347164650771788
2404	6	-0.014730490561547072
2404	7	0.04008382930578591
2404	8	-0.03969227511150564
2404	9	0.12036370739827618
2404	10	-0.010555560925261886
2404	11	0.09637751361904634
2404	12	-0.014115122926021684
2404	13	0.08289362377503551
2404	14	0.030835300859010317
2404	15	0.015481937185285233
2404	16	-0.0347980397509815
2404	17	-0.03245445920315149
2404	18	0.022024778846237325
2404	19	0.08351044381760175
2404	20	0.01101523970634588
2404	21	0.09052301233448645
2404	22	-0.02895521169693893
2404	23	-0.06313842125998548
2404	24	0.021830100854558054
2404	25	-0.06499413125585107
2404	26	0.01673710762317171
2404	27	-0.06643066103614359
2404	28	-0.022975590495995846
2404	29	-0.025210962778800194
2404	30	-0.029994921781963058
2404	31	-0.06106464577386106
2404	32	6.205978351374597E-4
2404	33	-0.1155469025153053
2404	34	0.09019661363609544
2404	35	-0.025633136932704303
2404	36	0.042133093528612164
2404	37	0.036190428588326995
2404	38	0.019831587478350343
2404	39	1.2866066681916664
2404	40	0.07242760914609647
2404	41	-0.029123486829121248
2404	42	-0.012835507748874875
2404	43	0.053999712895041796
2404	44	-0.04024117889718326
2404	45	-0.05559704969116552
2404	46	-0.011846418797162528
2404	47	-0.003554923891265105
2404	48	0.04908272130612801
2404	49	-0.030577310791237267
2404	50	0.03964707612256291
2404	51	-0.01245489898502683
2404	52	-0.0024279040730732504
2404	53	-0.07155846571266977
2404	54	0.002314656995501707
2404	55	0.10374736196958168
2404	56	0.053583638791799615
2404	57	-0.030585542074170746
2404	58	0.0034671358475293346
2404	59	0.03019450182980421
2404	60	-0.006270531468850038
2404	61	0.123037540497279
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	11.0
17	64.0
18	151.0
19	232.0
20	327.0
21	413.0
22	617.0
23	826.0
24	1220.0
25	1527.0
26	2002.0
27	2697.0
28	3543.0
29	4242.0
30	5293.0
31	6706.0
32	8742.0
33	10579.0
34	13361.0
35	17596.0
36	25616.0
37	38089.0
38	89648.0
39	579284.0
40	642535.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.284500612235643	15.136718884205694	25.56815605068844	29.010624452870225
2	32.71116237252999	15.934473059802649	28.179017522057226	23.17534704561014
3	29.314199881538244	19.736182095783615	22.0257097741943	28.923908248483844
4	26.269086021690093	21.358616220911017	21.71819374309	30.654104014308892
5	25.645939524036603	22.010317991482296	22.58366189750447	29.760080586976628
6	26.162320091361224	24.05893678512384	24.578409451654	25.200333671860935
7	25.877778251136174	23.77934230362765	23.70080298380702	26.642076461429156
8	24.502343811197797	25.42626305381215	24.105799266416643	25.965593868573418
9	24.3312476551581	25.593373837542483	24.687457483635924	25.38792102366349
10	25.69534439800951	23.863378688702568	23.98726879687107	26.45400811641685
11	25.08784997409508	24.69357296873132	23.866883067802178	26.351693989371423
12	25.560116592754046	24.03419999147955	24.112533171353142	26.293150244413265
13	25.006493408331625	22.804712634042502	23.951881439296596	28.236912518329277
14	25.105097016330408	23.874716385789537	23.76855431306611	27.251632284813944
15	25.086063427887435	23.84606293315156	23.82757905123402	27.240294587726975
16	25.478072893833804	24.135826985368187	23.274505573337034	27.111594547460978
17	25.53139442680039	23.6158046123126	23.34404344880377	27.508757512083235
18	26.119236842430748	24.0385289303673	23.548946556157333	26.293287671044617
19	25.594060970699267	23.52221707635836	23.919036474402226	26.964685478540147
20	25.493602103177164	23.751719550724857	23.30652597844326	27.448152367654714
21	25.443991089257224	23.96356270296196	24.139743644361864	26.452702563418956
22	26.33623349334374	23.29374530172704	23.146561379543495	27.22345982538572
23	25.50260354753106	24.449228418178244	23.25231117237285	26.795856861917844
24	25.655147108337534	24.93558126655132	23.187102235793866	26.22216938931728
25	25.75684281554185	23.59924470323406	23.06774720646015	27.576165274763937
26	25.802537170468113	24.15424215397005	23.827922617812415	26.21529805774942
27	25.736847240679385	24.72428782083965	23.158792349734288	26.380072588746685
28	25.83125933642177	24.24343203772086	22.96234098020919	26.962967645648177
29	25.725028550382667	25.510780432096812	23.045140525601894	25.719050491918626
30	26.545534252900733	25.388539443504598	22.745481755927553	25.320444547667115
31	26.10535675266367	24.948087090004822	23.261862323252174	25.68469383407933
32	26.105081899400957	25.510162012255705	22.638014130206237	25.746741958137097
33	26.269049920945275	26.503293775050313	22.687228453379014	24.540427850625395
34	26.718210815201033	25.661468733379966	22.23123130138897	25.389089150030024
35	26.853163767193788	26.919403403507953	21.956378038674604	24.271054790623655
36	27.17996429656117	27.07222181757714	21.484798553172425	24.26301533268926
37	27.301449438680926	26.95802028691932	21.84733000669268	23.893200267707076
38	27.47254559472062	27.535143425303815	20.748810228939025	24.24350075103654
39	28.53633968312253	27.972294664433267	20.376861749071207	23.114503903373
40	27.753514342530384	28.556773002813124	19.88315987802012	23.806552776636373
41	27.773784770655567	28.331530754018697	20.548304773788896	23.346379701536843
42	27.86393664082588	28.69949055947756	19.619506885761364	23.8170659139352
43	28.0785970390058	29.390815228519873	18.996689392450605	23.533898340023722
44	27.947285892744013	29.342715907544857	19.311052811680167	23.398945388030963
45	27.996347200138526	29.466880868976077	18.48017139849463	24.05660053239077
46	28.149165614207718	29.458635271094646	18.730287867564705	23.661911247132934
47	28.648092999349974	29.656941900143064	17.97938875382905	23.715576346677917
48	28.630364963904896	29.927534937285355	17.767820454854665	23.674279643955085
49	28.61641616082214	29.88884934055831	17.47276547733079	24.021969021288758
50	28.42848524244119	29.88850577397992	17.43950823254235	24.24350075103654
51	28.046576633899576	30.136423416948276	17.460328367192965	24.356671581959183
52	27.921518399364537	29.99432428012495	17.242163589913435	24.84199373059708
53	27.746917864225235	30.121375200814665	17.149263187115977	24.98244374784412
54	27.359031197219586	30.189538809967832	17.311357898801777	25.14007209401081
55	27.100875270215113	29.90444726321735	17.193514562412993	25.801162904154545
56	26.59212188093082	30.10804481757302	17.22670309388575	26.07313020761041
57	26.234469072823746	30.01136518241324	17.34083591122789	26.41332983353512
58	25.969991520776848	29.934818548747288	17.165891809510196	26.929298120965665
59	25.43766946421479	29.9489047784614	17.3404923446495	27.27293341267431
60	25.015975845895273	29.92842821038918	17.310670765644993	27.744925178070556
61	24.873258289230836	29.635365919019986	17.26916792297512	28.222207868774056
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	4.5
2	5.0
3	7.0
4	7.0
5	7.0
6	4.0
7	5.5
8	10.0
9	9.5
10	9.0
11	14.5
12	26.0
13	32.0
14	47.0
15	62.0
16	76.5
17	113.5
18	136.0
19	157.0
20	282.5
21	387.0
22	473.0
23	559.0
24	811.0
25	1529.0
26	1995.0
27	2563.5
28	3132.0
29	4176.0
30	6622.5
31	8025.0
32	9456.0
33	10887.0
34	13173.0
35	18311.0
36	21163.0
37	24227.5
38	27292.0
39	31890.5
40	41837.0
41	47185.0
42	52592.0
43	62927.0
44	67855.0
45	72541.0
46	77227.0
47	81201.5
48	87909.0
49	90642.0
50	93771.0
51	96900.0
52	99098.5
53	101942.0
54	102587.0
55	101889.5
56	101192.0
57	98588.0
58	91550.5
59	87117.0
60	81613.0
61	72705.0
62	69301.0
63	58858.5
64	48416.0
65	42277.5
66	31274.5
67	26410.0
68	22444.5
69	18479.0
70	15557.0
71	10692.5
72	8750.0
73	7283.0
74	5816.0
75	4803.5
76	3065.0
77	2339.0
78	1932.5
79	1526.0
80	1212.0
81	700.0
82	502.0
83	408.0
84	250.5
85	187.0
86	159.0
87	131.0
88	98.5
89	53.0
90	40.0
91	28.5
92	17.0
93	17.5
94	14.5
95	11.0
96	10.5
97	10.0
98	12.5
99	11.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03229525836893828
3	0.0
4	2.0613994703577628E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	6.871331567859209E-5
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.0659194322631005
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1455322.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.46607230615967
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.28959640128757	59.78850891050658
2	14.149873705332611	21.073710369286513
3	3.1537433827183574	7.045406483177334
4	0.9968750986463308	2.9693349270403093
5	0.45287885355490926	1.6862054727375286
6	0.2767110420292504	1.2363350678197769
7	0.18351594601599178	0.9565998193752097
8	0.12411827890647284	0.73940805852524
9	0.09087048790152609	0.6090091490313945
>10	0.27817609887806954	3.064951900141782
>50	0.002834365919774437	0.14106446106446055
>100	7.055464580191107E-4	0.08248665948638335
>500	0.0	0.0
>1k	0.0	0.0
>5k	1.0079235114558726E-4	0.606978721807445
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGATCATCTGGGGGGGGGGGGGGG	8087	0.5556845838927743	TruSeq Adapter, Index 8 (97% over 39bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.6881638565210998	1.3742663135718418E-4	0.0	0.0	6.871331567859209E-5
2	0.6907062492012077	1.3742663135718418E-4	0.0	0.0	6.871331567859209E-5
3	0.6914620956736722	1.3742663135718418E-4	0.0	0.0	6.871331567859209E-5
4	0.6926302220402083	1.3742663135718418E-4	0.0	0.0	6.871331567859209E-5
5	0.6942793416164945	1.3742663135718418E-4	6.871331567859209E-5	0.0	6.871331567859209E-5
6	0.6973714408220312	1.3742663135718418E-4	6.871331567859209E-5	0.0	6.871331567859209E-5
7	0.7021813729195325	1.3742663135718418E-4	6.871331567859209E-5	0.0	6.871331567859209E-5
8	0.7363318908117928	1.3742663135718418E-4	6.871331567859209E-5	0.0	6.871331567859209E-5
9	0.7412792495406515	1.3742663135718418E-4	6.871331567859209E-5	0.0	6.871331567859209E-5
10	0.7480818677928321	1.3742663135718418E-4	6.871331567859209E-5	0.0	6.871331567859209E-5
11	0.7565336056212989	1.3742663135718418E-4	6.871331567859209E-5	0.0	6.871331567859209E-5
12	0.7721315282803394	1.3742663135718418E-4	6.871331567859209E-5	0.0	6.871331567859209E-5
13	0.7894472838313445	1.3742663135718418E-4	1.3742663135718418E-4	0.0	6.871331567859209E-5
14	0.8106109850603509	1.3742663135718418E-4	1.3742663135718418E-4	0.0	6.871331567859209E-5
15	0.8402264241178241	1.3742663135718418E-4	1.3742663135718418E-4	6.871331567859209E-5	6.871331567859209E-5
16	0.8719719759613337	1.3742663135718418E-4	1.3742663135718418E-4	6.871331567859209E-5	6.871331567859209E-5
17	0.9090771664277734	1.3742663135718418E-4	1.3742663135718418E-4	6.871331567859209E-5	6.871331567859209E-5
18	0.951748135464179	1.3742663135718418E-4	1.3742663135718418E-4	6.871331567859209E-5	6.871331567859209E-5
19	1.0197056046703066	1.3742663135718418E-4	1.3742663135718418E-4	6.871331567859209E-5	6.871331567859209E-5
20	1.107589935423226	1.3742663135718418E-4	1.3742663135718418E-4	6.871331567859209E-5	6.871331567859209E-5
21	1.2342972895345496	1.3742663135718418E-4	1.3742663135718418E-4	6.871331567859209E-5	6.871331567859209E-5
22	1.413158050245925	1.3742663135718418E-4	1.3742663135718418E-4	6.871331567859209E-5	6.871331567859209E-5
23	1.662656099474893	1.3742663135718418E-4	1.3742663135718418E-4	6.871331567859209E-5	6.871331567859209E-5
24	2.023813286681573	1.3742663135718418E-4	1.3742663135718418E-4	6.871331567859209E-5	6.871331567859209E-5
25	2.4858416213044263	1.3742663135718418E-4	1.3742663135718418E-4	6.871331567859209E-5	1.3742663135718418E-4
26	3.084197174233606	2.7485326271436836E-4	1.3742663135718418E-4	6.871331567859209E-5	1.3742663135718418E-4
27	3.8157191329478977	2.7485326271436836E-4	1.3742663135718418E-4	6.871331567859209E-5	1.3742663135718418E-4
28	4.682537610233337	2.7485326271436836E-4	2.0613994703577628E-4	6.871331567859209E-5	1.3742663135718418E-4
29	5.7328893536962955	2.7485326271436836E-4	2.0613994703577628E-4	6.871331567859209E-5	1.3742663135718418E-4
30	6.94451124905691	2.7485326271436836E-4	2.0613994703577628E-4	6.871331567859209E-5	1.3742663135718418E-4
31	8.28902469693992	2.7485326271436836E-4	2.0613994703577628E-4	6.871331567859209E-5	1.3742663135718418E-4
32	9.79570156982441	2.7485326271436836E-4	2.0613994703577628E-4	6.871331567859209E-5	1.3742663135718418E-4
33	11.45416615704291	2.7485326271436836E-4	2.0613994703577628E-4	6.871331567859209E-5	1.3742663135718418E-4
34	13.247514982938483	2.7485326271436836E-4	2.0613994703577628E-4	6.871331567859209E-5	1.3742663135718418E-4
35	15.14283436930109	2.7485326271436836E-4	2.0613994703577628E-4	6.871331567859209E-5	1.3742663135718418E-4
36	17.179084766120486	2.7485326271436836E-4	2.0613994703577628E-4	6.871331567859209E-5	1.3742663135718418E-4
37	19.298684414858016	2.7485326271436836E-4	2.0613994703577628E-4	6.871331567859209E-5	1.3742663135718418E-4
38	21.50211430872343	2.7485326271436836E-4	4.1227989407155257E-4	6.871331567859209E-5	1.3742663135718418E-4
39	23.776799910947542	2.7485326271436836E-4	4.1227989407155257E-4	6.871331567859209E-5	2.0613994703577628E-4
40	26.186644605111447	2.7485326271436836E-4	4.1227989407155257E-4	6.871331567859209E-5	2.7485326271436836E-4
41	28.633869343004502	2.7485326271436836E-4	4.1227989407155257E-4	6.871331567859209E-5	2.7485326271436836E-4
42	31.115244598789822	2.7485326271436836E-4	4.1227989407155257E-4	6.871331567859209E-5	2.7485326271436836E-4
43	33.627953126524574	2.7485326271436836E-4	4.8099320975014464E-4	6.871331567859209E-5	2.7485326271436836E-4
44	36.143272760255115	2.7485326271436836E-4	4.8099320975014464E-4	6.871331567859209E-5	2.7485326271436836E-4
45	38.65872982061702	2.7485326271436836E-4	4.8099320975014464E-4	6.871331567859209E-5	2.7485326271436836E-4
46	41.144708868552804	2.7485326271436836E-4	4.8099320975014464E-4	6.871331567859209E-5	2.7485326271436836E-4
47	43.61144818809858	2.7485326271436836E-4	4.8099320975014464E-4	6.871331567859209E-5	2.7485326271436836E-4
48	46.070354189656996	2.7485326271436836E-4	4.8099320975014464E-4	6.871331567859209E-5	2.7485326271436836E-4
49	48.466387507369504	2.7485326271436836E-4	4.8099320975014464E-4	6.871331567859209E-5	2.7485326271436836E-4
50	50.772681234805766	2.7485326271436836E-4	4.8099320975014464E-4	6.871331567859209E-5	2.7485326271436836E-4
>>END_MODULE