##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-30C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 832504 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.55477931637566 40.0 40.0 40.0 40.0 40.0 2 39.36875738735189 40.0 40.0 40.0 40.0 40.0 3 39.33048730096192 40.0 40.0 40.0 40.0 40.0 4 39.23730336430816 40.0 40.0 40.0 40.0 40.0 5 39.25822818869339 40.0 40.0 40.0 40.0 40.0 6 39.378088273449734 40.0 40.0 40.0 40.0 40.0 7 39.38695309572086 40.0 40.0 40.0 40.0 40.0 8 39.377660647876766 40.0 40.0 40.0 40.0 40.0 9 39.40612897956046 40.0 40.0 40.0 40.0 40.0 10-11 39.40689293985375 40.0 40.0 40.0 40.0 40.0 12-13 39.3291755955527 40.0 40.0 40.0 40.0 40.0 14-15 39.358422301874825 40.0 40.0 40.0 40.0 40.0 16-17 39.46818273545833 40.0 40.0 40.0 40.0 40.0 18-19 39.49767208325726 40.0 40.0 40.0 40.0 40.0 20-21 39.48112201262697 40.0 40.0 40.0 40.0 40.0 22-23 39.51963233810288 40.0 40.0 40.0 40.0 40.0 24-25 39.49623064874163 40.0 40.0 40.0 40.0 40.0 26-27 39.472084218213965 40.0 40.0 40.0 40.0 40.0 28-29 39.49151475548466 40.0 40.0 40.0 40.0 40.0 30-31 39.46385970453542 40.0 40.0 40.0 40.0 40.0 32-33 39.46765918376309 40.0 40.0 40.0 40.0 40.0 34-35 39.46526597439222 40.0 40.0 40.0 40.0 40.0 36-37 39.449687644846605 40.0 40.0 40.0 40.0 40.0 38-39 39.38803344326604 40.0 40.0 40.0 40.0 40.0 40-41 39.43057354988103 40.0 40.0 40.0 40.0 40.0 42-43 39.40846206591456 40.0 40.0 40.0 40.0 40.0 44-45 39.314012169305485 40.0 40.0 40.0 40.0 40.0 46-47 39.3053204640637 40.0 40.0 40.0 40.0 40.0 48-49 39.38240334773115 40.0 40.0 40.0 40.0 40.0 50-51 39.342709268223004 40.0 40.0 40.0 40.0 40.0 52-53 39.30610205896231 40.0 40.0 40.0 40.0 40.0 54-55 39.22026142710172 40.0 40.0 40.0 40.0 40.0 56-57 39.23187863113071 40.0 40.0 40.0 40.0 40.0 58-59 39.17385867220136 40.0 40.0 40.0 40.0 40.0 60-61 39.1000531893109 40.0 40.0 40.0 40.0 40.0 62-63 39.381424152830675 40.0 40.0 40.0 40.0 40.0 64-65 39.37914440848627 40.0 40.0 40.0 40.0 40.0 66-67 39.39370340074041 40.0 40.0 40.0 40.0 40.0 68-69 39.38513178786029 40.0 40.0 40.0 40.0 40.0 70-71 39.383396463167344 40.0 40.0 40.0 40.0 40.0 72-73 39.39207528715642 40.0 40.0 40.0 40.0 40.0 74-75 39.399608717514 40.0 40.0 40.0 40.0 40.0 76-77 39.39416867248631 40.0 40.0 40.0 40.0 40.0 78-79 39.37460684661381 40.0 40.0 40.0 40.0 40.0 80-81 39.38445840265449 40.0 40.0 40.0 40.0 40.0 82-83 39.386636836053725 40.0 40.0 40.0 40.0 40.0 84-85 39.37252480133673 40.0 40.0 40.0 40.0 40.0 86-87 39.352774488565544 40.0 40.0 40.0 40.0 40.0 88-89 39.42100777938737 40.0 40.0 40.0 40.0 40.0 90-91 39.470695480779696 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.045251591478468356 1101 2 0.04488157501340595 1101 3 -0.03014960915572118 1101 4 0.03085886450081432 1101 5 -0.007593362354093358 1101 6 -0.012285712780418123 1101 7 -0.0835110378628201 1101 8 -0.04048923656446135 1101 9 -0.19363803921091005 1101 10-11 0.028403542733528298 1101 12-13 -0.0033088884218841486 1101 14-15 -0.036193273717351815 1101 16-17 0.034327225257605676 1101 18-19 0.05774082230527 1101 20-21 0.0810378412328987 1101 22-23 -0.08426473169225801 1101 24-25 0.0032274086666248536 1101 26-27 -0.042565822764828454 1101 28-29 0.008981531343437155 1101 30-31 0.0027388854449839073 1101 32-33 0.011685422151103353 1101 34-35 -0.11290290393512947 1101 36-37 -0.16596536624604852 1101 38-39 -0.048441707574696125 1101 40-41 -0.14816002145108342 1101 42-43 0.005423626107102564 1101 44-45 -0.05329396193319269 1101 46-47 -0.10478001269645887 1101 48-49 -0.09215509425776958 1101 50-51 -0.0883610758231228 1101 52-53 0.020848905353858527 1101 54-55 -0.08035106282699189 1101 56-57 -0.12048260477384787 1101 58-59 0.04300021987769753 1101 60-61 0.1352088195373824 1101 62-63 -0.01171626224275002 1101 64-65 -0.11253976515113351 1101 66-67 -0.08646863956000317 1101 68-69 0.09746413164671708 1101 70-71 0.12898412589566277 1101 72-73 -9.17977846704332E-4 1101 74-75 -0.2190103234334586 1101 76-77 -0.03339310644840765 1101 78-79 -0.18579680240905816 1101 80-81 -0.17229622779839104 1101 82-83 -0.2407918044147408 1101 84-85 0.1622221388309768 1101 86-87 -0.11057949628880692 1101 88-89 -0.5798657923388433 1101 90-91 0.24747727402904474 1102 1 0.04511944111077071 1102 2 0.15246773015587678 1102 3 0.12024881428001066 1102 4 -0.06596998023028533 1102 5 -0.03619958590027039 1102 6 -0.07304232985589465 1102 7 -0.02487276588427534 1102 8 -0.11521208573454089 1102 9 0.07810477069188693 1102 10-11 0.01178142629714074 1102 12-13 -0.029337533798518223 1102 14-15 -0.1007554891947322 1102 16-17 0.030498379710827805 1102 18-19 -0.11544388156281826 1102 20-21 -0.01688606637560497 1102 22-23 -0.01184171848706228 1102 24-25 -0.0455397993186466 1102 26-27 -0.04095932269997604 1102 28-29 -0.007392615547523462 1102 30-31 -0.05483558777780928 1102 32-33 0.013608367025760515 1102 34-35 -0.009269699810367626 1102 36-37 -0.11717088165134015 1102 38-39 -0.02851336242927971 1102 40-41 -0.0670512084871504 1102 42-43 -0.004824248021712663 1102 44-45 -0.11195458135707526 1102 46-47 0.12091196312839259 1102 48-49 0.026677376902384253 1102 50-51 -0.046748502511853474 1102 52-53 0.022936882184929175 1102 54-55 -0.05497583779012416 1102 56-57 0.08056931659611877 1102 58-59 0.18116609072465195 1102 60-61 -0.14230856346173226 1102 62-63 -0.09744263930336672 1102 64-65 0.016786169824520414 1102 66-67 -0.09313271657109823 1102 68-69 -0.49078722889177584 1102 70-71 -0.23663314234011779 1102 72-73 0.04457057666454034 1102 74-75 -0.25430535872849447 1102 76-77 -0.027569880714239048 1102 78-79 -0.3425228217660603 1102 80-81 -0.399845517840447 1102 82-83 -0.8249395708994669 1102 84-85 -0.5776067511618521 1102 86-87 -0.9632872460553799 1102 88-89 -0.4800850905844527 1102 90-91 -0.8239512973995247 1103 1 0.07071457436263984 1103 2 0.13369454308195117 1103 3 0.002802656472454146 1103 4 -0.08614910272122245 1103 5 -0.15627752843226972 1103 6 -0.017407569761402897 1103 7 -0.1021107504679577 1103 8 0.0346156081917357 1103 9 0.02509547651095545 1103 10-11 0.019564621577622177 1103 12-13 0.03805574346063878 1103 14-15 0.03878004661278567 1103 16-17 -0.09505323273123167 1103 18-19 -0.08727225318155973 1103 20-21 -0.03267322039108933 1103 22-23 -0.03896955829888782 1103 24-25 -0.04006731418562026 1103 26-27 -0.01724916971366497 1103 28-29 -0.01739371344091012 1103 30-31 -0.022123228784181492 1103 32-33 -0.010740605832118888 1103 34-35 0.012931906259673553 1103 36-37 0.02830541100341577 1103 38-39 -0.00927988830142823 1103 40-41 0.07055265710726388 1103 42-43 0.03376254536211576 1103 44-45 0.009243281556202021 1103 46-47 -0.07462635284106511 1103 48-49 -0.06370432883210952 1103 50-51 0.06657027879090549 1103 52-53 -0.04793599598279741 1103 54-55 -0.13036605097522624 1103 56-57 0.10917976020276399 1103 58-59 -0.06752622865472091 1103 60-61 0.10910135069470783 1103 62-63 0.038672741690056966 1103 64-65 0.17421083769378498 1103 66-67 -0.08562093474527899 1103 68-69 -0.33922315117109747 1103 70-71 -0.4779560032663639 1103 72-73 -0.20652005226623515 1103 74-75 -0.43644306989846626 1103 76-77 -0.6634824813942188 1103 78-79 -0.5979415877415448 1103 80-81 -0.2898811960963954 1103 82-83 -0.860025598370477 1103 84-85 -0.37936863226799034 1103 86-87 -0.6537797221155799 1103 88-89 0.2280045780315305 1103 90-91 -1.0025227259709553 1104 1 -0.060114937544028635 1104 2 0.06599115958536572 1104 3 -0.022044870154125817 1104 4 -0.11049973895328691 1104 5 -0.046431675334041245 1104 6 -0.04378861591112582 1104 7 -0.05758907042966399 1104 8 0.03248446708971642 1104 9 -0.045331407589735306 1104 10-11 0.014563769309113184 1104 12-13 -0.058253041219501256 1104 14-15 -0.004523462647433973 1104 16-17 -0.03323322695421638 1104 18-19 -0.0110454328647549 1104 20-21 -0.01152192685046316 1104 22-23 0.03176966764586098 1104 24-25 0.05841959442507516 1104 26-27 0.061996955095651174 1104 28-29 0.016711309718807854 1104 30-31 0.03387692005741627 1104 32-33 0.019401686506277827 1104 34-35 -0.053147937137005385 1104 36-37 -0.04666611473777493 1104 38-39 0.03624551174382873 1104 40-41 -0.03330654515048792 1104 42-43 -0.03367832572937601 1104 44-45 0.008432161583883158 1104 46-47 -0.10838178491725614 1104 48-49 -0.22351263676677746 1104 50-51 -0.04736931645179965 1104 52-53 -0.16199497605105506 1104 54-55 -0.19574931978620214 1104 56-57 -0.035546411998382155 1104 58-59 0.0527632422892097 1104 60-61 -0.2440684096633987 1104 62-63 -0.1389311236047135 1104 64-65 0.05852674204805197 1104 66-67 -0.07182204489590305 1104 68-69 -0.260852035279612 1104 70-71 -0.3518051051158366 1104 72-73 -0.280477984227538 1104 74-75 -0.1426832455503373 1104 76-77 -0.06551798117888552 1104 78-79 0.37955422764173363 1104 80-81 0.20465392071281485 1104 82-83 -0.04936476034864512 1104 84-85 0.18280323101284068 1104 86-87 0.08233869662938531 1104 88-89 0.5243008743278281 1104 90-91 0.7474772740290447 1201 1 0.0015409844735643219 1201 2 0.04707721816701138 1201 3 0.08183213217213847 1201 4 0.08931924263848146 1201 5 0.030051751256252146 1201 6 0.09382390466461743 1201 7 0.013849107115831316 1201 8 -0.01441656518943546 1201 9 -0.023911513422149255 1201 10-11 0.03456174646730403 1201 12-13 0.0383354323611087 1201 14-15 0.04993173529652495 1201 16-17 -0.027953461633508425 1201 18-19 -0.00590682189020697 1201 20-21 -0.01176866909335672 1201 22-23 0.03790673259130273 1201 24-25 0.010124060941294033 1201 26-27 0.05135702945916876 1201 28-29 0.047390706704476315 1201 30-31 0.072413908979577 1201 32-33 -0.04459426673990663 1201 34-35 0.03357900930769375 1201 36-37 0.05068467240301544 1201 38-39 0.09197937731617856 1201 40-41 -0.025340600599243146 1201 42-43 0.056955545478011516 1201 44-45 0.0182825373220723 1201 46-47 0.004529043934823562 1201 48-49 0.058019753850466316 1201 50-51 0.05304568799770237 1201 52-53 -0.18721743849572903 1201 54-55 0.17158240969937566 1201 56-57 -0.03176578820641396 1201 58-59 0.1388016525080218 1201 60-61 0.07921784002758159 1201 62-63 -0.05480451964979949 1201 64-65 -0.05347821877072789 1201 66-67 -0.08145582114512706 1201 68-69 -0.11684753258936098 1201 70-71 0.11287136439545264 1201 72-73 -0.16192348917554256 1201 74-75 0.10539769113848507 1201 76-77 -0.1380727097783705 1201 78-79 0.1738189722339598 1201 80-81 -0.07154574708425088 1201 82-83 -0.018779432018732223 1201 84-85 -0.31700069055578695 1201 86-87 0.06016267445335899 1201 88-89 0.5243008743278281 1201 90-91 -0.7070681805164085 1202 1 0.03841625084874778 1202 2 0.01990620455542569 1202 3 -0.013919872906498654 1202 4 -0.03987905375252865 1202 5 7.065200798521687E-4 1202 6 -0.04912307661535209 1202 7 0.04866490215819397 1202 8 0.04473288209138104 1202 9 0.0026082780675551476 1202 10-11 0.07324687386542195 1202 12-13 -0.029638298202812052 1202 14-15 0.09413213288477351 1202 16-17 0.013431927743674521 1202 18-19 0.05135080277337778 1202 20-21 0.04049823958354182 1202 22-23 -0.01113685011390686 1202 24-25 -0.08664426790650737 1202 26-27 -0.023115510426322317 1202 28-29 -0.03586960629940705 1202 30-31 0.025988626659142255 1202 32-33 -0.03947850622406435 1202 34-35 -0.0020039822882225167 1202 36-37 -0.2494658782928596 1202 38-39 -0.1463023666685217 1202 40-41 -0.10860166719908904 1202 42-43 -0.061561242326185095 1202 44-45 -0.15541266654496866 1202 46-47 -0.11692405348088641 1202 48-49 -0.007881422915595238 1202 50-51 -0.07712752155406122 1202 52-53 0.11190196660218277 1202 54-55 -0.03906961813103038 1202 56-57 -0.06751563746273348 1202 58-59 -0.11073728765272506 1202 60-61 -0.04789483432019637 1202 62-63 -0.0751008441503771 1202 64-65 -0.37255844722113096 1202 66-67 -0.251715312727562 1202 68-69 -0.18276723380317605 1202 70-71 -0.15994921489994596 1202 72-73 -0.20195919512860172 1202 74-75 0.04026180082939135 1202 76-77 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-0.009639463925957159 2401 36-37 0.037616667602954124 2401 38-39 0.1337040153215696 2401 40-41 -0.058412322487932045 2401 42-43 0.04209339752745933 2401 44-45 0.05206218537748697 2401 46-47 -0.021251362929902484 2401 48-49 -2.662142212770391E-4 2401 50-51 0.09109328915333492 2401 52-53 0.022007935897583764 2401 54-55 0.1046298109695698 2401 56-57 0.07348211395265025 2401 58-59 0.025651125628932903 2401 60-61 0.08987422642458398 2401 62-63 0.05894590054207072 2401 64-65 -0.008303776992001133 2401 66-67 0.046430761347927785 2401 68-69 -0.010783644573891138 2401 70-71 -0.08681175257613205 2401 72-73 0.11570231355138105 2401 74-75 0.15849295858109969 2401 76-77 0.19136515142415078 2401 78-79 0.08001923485250728 2401 80-81 0.19162027165979367 2401 82-83 0.31173972202982725 2401 84-85 0.2843786197890381 2401 86-87 0.2240636383543304 2401 88-89 0.3191726691996237 2401 90-91 -0.7763322497804737 2402 1 -0.009554955806201804 2402 2 -0.06881217638422754 2402 3 0.05466817737564611 2402 4 -0.04276729624155706 2402 5 0.07564322200157392 2402 6 0.08269452898708352 2402 7 0.002232035808795274 2402 8 0.0925207898457785 2402 9 0.07811372050313281 2402 10-11 0.007755204543492766 2402 12-13 0.04285641696439768 2402 14-15 0.020741480100852527 2402 16-17 0.023387456346860347 2402 18-19 -0.016225677408073125 2402 20-21 -0.0034121255410184403 2402 22-23 0.04854235967939502 2402 24-25 -0.03086658377675633 2402 26-27 -0.0026108731354455017 2402 28-29 0.038410131805868275 2402 30-31 0.07276325123901017 2402 32-33 -0.005820693617231143 2402 34-35 0.033555800472399255 2402 36-37 0.06601346623701687 2402 38-39 -0.4013018190415565 2402 40-41 0.02261187479731319 2402 42-43 0.05366543996117201 2402 44-45 0.038974380159167765 2402 46-47 -0.0460235639006612 2402 48-49 -0.05019782159880748 2402 50-51 -0.015067133051488213 2402 52-53 0.07323475841338478 2402 54-55 0.06903987224037422 2402 56-57 -0.001420860588147832 2402 58-59 -0.006925273128864262 2402 60-61 -0.004063951435483659 2402 62-63 -0.06568717972402993 2402 64-65 -0.020840500192775835 2402 66-67 0.22961850757626223 2402 68-69 0.08652928749215505 2402 70-71 0.22052435350341426 2402 72-73 0.012661254515137443 2402 74-75 0.22931561962932534 2402 76-77 0.0693209729704094 2402 78-79 0.002495086665277313 2402 80-81 -0.30283220105977904 2402 82-83 0.10997109796245041 2402 84-85 -0.10274781699256863 2402 86-87 0.3675951068857941 2402 88-89 -0.13125468122772332 2402 90-91 0.7474772740290447 2403 1 -0.06398565847625548 2403 2 0.04353724185366303 2403 3 0.05539185490702181 2403 4 0.1378236550126033 2403 5 0.1163427075310608 2403 6 0.020246971745841336 2403 7 -0.006492877354240534 2403 8 0.006479089012678685 2403 9 -0.03130736350333052 2403 10-11 0.10162654960318207 2403 12-13 0.06640218522966279 2403 14-15 0.12186803950011438 2403 16-17 0.08214699149251459 2403 18-19 0.05714472487507294 2403 20-21 0.01053493666661609 2403 22-23 -0.0069467430732714774 2403 24-25 -0.0011463450152433552 2403 26-27 0.06969266880618363 2403 28-29 0.07167348361294046 2403 30-31 -0.06079234828096958 2403 32-33 0.06117971406673206 2403 34-35 0.0681800233157972 2403 36-37 0.05334584240603846 2403 38-39 0.09111864291682537 2403 40-41 0.02834646744535263 2403 42-43 0.0230003874426572 2403 44-45 0.05179296524358534 2403 46-47 0.001389790624770626 2403 48-49 0.04762966476594244 2403 50-51 -0.043860426006396835 2403 52-53 -0.044462169838517696 2403 54-55 0.0800579368788874 2403 56-57 0.014054049836346394 2403 58-59 0.040584211789486346 2403 60-61 0.10068012962207717 2403 62-63 0.12064094943212922 2403 64-65 -0.05080369089207437 2403 66-67 0.16324740477644895 2403 68-69 0.08112859051409771 2403 70-71 -0.040015066042577985 2403 72-73 0.09157155516457038 2403 74-75 -0.02804670634821349 2403 76-77 0.1757548913862479 2403 78-79 -0.14975714162906684 2403 80-81 0.13766862658194867 2403 82-83 -0.35205374773379106 2403 84-85 -0.13849463159394304 2403 86-87 -0.1937685294778433 2403 88-89 -0.7693499193229698 2403 90-91 -1.891411614859841 2404 1 0.020047089792022632 2404 2 -0.029716005380940658 2404 3 -0.012157213958239765 2404 4 -0.05390444211204226 2404 5 -0.022956038052122096 2404 6 -0.06371000085137268 2404 7 0.029166165071899286 2404 8 -0.050368313611464544 2404 9 0.0882435832807289 2404 10-11 0.033170231806600725 2404 12-13 0.04914962985988325 2404 14-15 0.053893967957996836 2404 16-17 -0.045357517983561024 2404 18-19 0.06337233192879665 2404 20-21 0.05277451957095991 2404 22-23 -0.0037604584697348287 2404 24-25 0.007327351766768686 2404 26-27 0.05747301819791062 2404 28-29 0.01944221955942993 2404 30-31 0.004799319689809067 2404 32-33 0.04960607623319646 2404 34-35 0.022055642127746466 2404 36-37 0.01739769551517867 2404 38-39 0.07611211119407812 2404 40-41 0.02810506078363062 2404 42-43 -0.04960700492717507 2404 44-45 0.004467987844329002 2404 46-47 1.5162375859745225E-4 2404 48-49 0.016711317314822338 2404 50-51 0.11062716064297717 2404 52-53 -0.09824207227296711 2404 54-55 0.025194777127950374 2404 56-57 -0.15314422554487805 2404 58-59 -0.08177780627927689 2404 60-61 0.09642311562686956 2404 62-63 0.18310985880105335 2404 64-65 0.24540607391079305 2404 66-67 0.28255022571806165 2404 68-69 0.2913230625790675 2404 70-71 0.044209347334778215 2404 72-73 0.2575416973221323 2404 74-75 -0.07833860540043247 2404 76-77 0.22277261711170837 2404 78-79 0.44317215130021737 2404 80-81 0.07503104688068873 2404 82-83 -0.16331310842181068 2404 84-85 -0.09809237047099373 2404 86-87 0.03491485610209821 2404 88-89 0.31377455853835556 2404 90-91 0.7474772740290447 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 23.0 31 543.0 32 3485.0 33 6400.0 34 8292.0 35 10414.0 36 14738.0 37 21658.0 38 54505.0 39 316795.0 40 395651.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 30.6844171319297 14.527738004862439 25.042161959582177 29.745682903625685 2 32.83522022854177 15.809194529605675 27.486400899558873 23.869184342293686 3 29.89595245188011 18.785975803119264 21.60374004208989 29.714331702910734 4 26.57777019689995 21.124342946099958 20.991490731576064 31.306396125424023 5 25.95110654122983 21.873648655141597 22.50607804887424 29.669166754754333 6 25.725642159076713 23.99712193575046 24.32156482131017 25.955671083862658 7 25.46173952317346 23.682889211343127 23.597364096749086 27.258007168734323 8 24.973693820089753 24.501263657592034 23.71724340063231 26.8077991216859 9 24.69021169868253 24.72156289939748 24.31399729010311 26.27422811181688 10-11 25.431109039716326 23.753399383066025 23.629375954950365 27.18611562226728 12-13 25.322400853329235 23.171600376694887 23.74583185185897 27.76016691811691 14-15 25.519757262427568 23.197125779575835 23.592018777086956 27.69109818090964 16-17 25.92528083949146 23.197005660032865 23.06085015807732 27.816863342398356 18-19 25.93140693618289 23.377365153801062 23.02727674581744 27.663951164198608 20-21 25.89507077443472 23.395503204789406 23.116765805329464 27.59266021544641 22-23 25.993989218069824 22.948838684258575 23.042411808231552 28.014760289440048 24-25 25.967382739302153 23.161990813257354 23.1103994695521 27.760226977888397 26-27 26.138072609861336 23.47105839731701 23.073162411231657 27.317706581590002 28-29 26.263777711578562 23.189918606997683 22.981270960860247 27.565032720563504 30-31 26.278526567538528 23.30191959917909 23.008943634859936 27.410610198422447 32-33 26.171302145656533 23.284715950137517 23.341667682924943 27.202314221281004 34-35 26.268856690128732 23.034194719769737 23.260614566300184 27.436334023801344 36-37 26.27982202016583 23.335728634973187 23.292901465251997 27.091547879608985 38-39 26.442273564532286 23.34978202976865 23.306751478809286 26.901192926889777 40-41 26.21840832710905 23.075557691839474 23.480273538589376 27.2257604424621 42-43 26.21287099258312 23.19410185753409 23.599284661531687 26.993742488351103 44-45 26.103059044166837 23.41474352915886 23.665015483318598 26.8171819433557 46-47 26.24626737443788 23.11182778425653 23.606461038862008 27.03544380244358 48-49 26.253957308794178 23.385827921221058 23.576070733490482 26.78414403649428 50-51 26.250657847149995 23.421603326274727 23.68898233851879 26.638756488056487 52-53 26.27124676038804 23.186759355273605 23.749897072102563 26.792096812235787 54-55 26.217629915835033 23.330942669581496 23.782605488691953 26.668821925891518 56-57 26.205973351300077 23.482593239421636 23.888972615490996 26.422460793787288 58-59 26.32684979981763 23.211895401944858 23.81274444088833 26.64851035734918 60-61 26.405145905528265 23.429853508874665 23.652380394669457 26.51262019092761 62-63 26.303691695332994 23.498741317728307 23.837201639581934 26.36036534735677 64-65 26.287216690605447 23.249628746013585 23.720695279596853 26.742459283784115 66-67 26.05757267277457 23.42565886211967 23.954445198506956 26.562323266598803 68-69 26.163739163834155 23.310714536466097 23.954999194644177 26.57054710505557 70-71 26.39748382423715 23.22358282816989 23.765124520560597 26.613808827032358 72-73 26.16790089279535 23.45606385678363 23.796917895125382 26.57911735529564 74-75 26.593250001729352 23.249379163409724 23.723912757759592 26.433458077101335 76-77 26.563746106899703 23.15376801739408 23.55755912055809 26.724926755148125 78-79 26.296254174377847 23.29276992586925 23.6008726129796 26.810103286773295 80-81 26.64275783209241 23.203264075676657 23.40986759183461 26.744110500396317 82-83 26.46565883417692 23.001374317031477 23.428753393892666 27.10421345489894 84-85 26.378023965634185 23.131358806240108 22.993443364232423 27.497173863893288 86-87 27.08519289893881 22.291646004826607 22.962742570002316 27.660418526232274 88-89 25.74760098192368 21.328944432046416 24.085025663914305 28.8384289221156 90-91 22.573488630061007 21.08985024958403 26.552967276760953 29.783693843594012 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.5 8 2.5 9 3.0 10 8.0 11 14.0 12 18.5 13 33.0 14 66.5 15 99.0 16 128.0 17 170.5 18 238.0 19 361.5 20 518.0 21 697.0 22 951.5 23 1305.0 24 1729.5 25 2201.5 26 2888.0 27 3736.0 28 4670.0 29 5771.0 30 6944.0 31 8186.5 32 9594.5 33 11190.5 34 13137.5 35 15279.0 36 17584.5 37 20022.5 38 22671.0 39 25669.5 40 28690.0 41 31757.5 42 34612.0 43 37215.5 44 40009.0 45 42965.5 46 45896.5 47 48721.5 48 51056.0 49 53011.5 50 54837.0 51 56509.5 52 57460.5 53 57892.5 54 58364.0 55 58187.0 56 57529.0 57 57087.5 58 56386.5 59 54995.5 60 53439.0 61 51599.0 62 49517.0 63 47218.5 64 44311.0 65 41139.0 66 37760.0 67 34559.0 68 31363.5 69 27837.0 70 24541.0 71 21417.5 72 18397.5 73 15572.5 74 12991.0 75 10910.0 76 9111.5 77 7353.0 78 5953.0 79 4782.0 80 3726.5 81 2947.0 82 2354.0 83 1843.5 84 1419.5 85 1074.0 86 788.0 87 570.0 88 412.5 89 285.0 90 205.5 91 161.0 92 126.0 93 86.0 94 58.5 95 40.0 96 17.5 97 8.5 98 7.5 99 4.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011531476125039639 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0645772784516665 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 3.0430562022049984E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 6.917351484463628E-4 76-77 0.0 78-79 0.0010338909451831021 80-81 0.0012993762993762994 82-83 0.003351880404907153 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 16051.0 32-33 36347.0 34-35 40716.0 36-37 44195.0 38-39 46582.0 40-41 48127.0 42-43 48803.0 44-45 48106.0 46-47 46985.0 48-49 44723.0 50-51 43248.0 52-53 40616.0 54-55 37208.0 56-57 34398.0 58-59 31369.0 60-61 28702.0 62-63 25960.0 64-65 23457.0 66-67 21034.0 68-69 18692.0 70-71 16689.0 72-73 14835.0 74-75 13111.0 76-77 11588.0 78-79 10161.0 80-81 8910.0 82-83 7943.0 84-85 7187.0 86-87 6364.0 88-89 5542.0 90-91 4855.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.91563593921549 #Duplication Level Percentage of deduplicated Percentage of total 1 99.92731303852732 99.84301029941516 2 0.06494578378391277 0.1297819857668087 3 0.005655967621201903 0.01695358805172 4 0.00120022908664715 0.0047968660986037505 5 2.543525969001625E-4 0.0012706900736035335 6 2.2429315092486766E-4 0.0013446235686881156 7 4.063352331033395E-4 0.0028419470254020677 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.002762749488290747 1.2011954296916291E-4 0.0 0.0 1.2011954296916291E-4 2 0.002762749488290747 1.2011954296916291E-4 0.0 0.0 1.2011954296916291E-4 3 0.002762749488290747 1.2011954296916291E-4 0.0 0.0 1.2011954296916291E-4 4 0.002762749488290747 1.2011954296916291E-4 0.0 0.0 1.2011954296916291E-4 5 0.002762749488290747 1.2011954296916291E-4 0.0 0.0 1.2011954296916291E-4 6 0.002762749488290747 1.2011954296916291E-4 0.0 0.0 1.2011954296916291E-4 7 0.003123108117198236 1.2011954296916291E-4 0.0 0.0 1.2011954296916291E-4 8 0.008408368007841404 1.2011954296916291E-4 0.0 0.0 1.2011954296916291E-4 9 0.00864860709377973 1.2011954296916291E-4 0.0 0.0 1.2011954296916291E-4 10-11 0.00864860709377973 1.2011954296916291E-4 0.0 0.0 1.2011954296916291E-4 12-13 0.00864860709377973 1.2011954296916291E-4 6.0059771484581455E-5 0.0 1.2011954296916291E-4 14-15 0.00864860709377973 1.2011954296916291E-4 1.2011954296916291E-4 6.0059771484581455E-5 1.2011954296916291E-4 16-17 0.008708666865264312 1.2011954296916291E-4 1.2011954296916291E-4 1.2011954296916291E-4 1.2011954296916291E-4 18-19 0.008768726636748892 1.2011954296916291E-4 1.2011954296916291E-4 1.2011954296916291E-4 1.2011954296916291E-4 20-21 0.008828786408233473 1.2011954296916291E-4 1.2011954296916291E-4 1.2011954296916291E-4 1.2011954296916291E-4 22-23 0.009069025494171799 1.2011954296916291E-4 1.2011954296916291E-4 1.2011954296916291E-4 1.2011954296916291E-4 24-25 0.009129085265656381 1.2011954296916291E-4 1.2011954296916291E-4 1.2011954296916291E-4 1.8017931445374436E-4 26-27 0.009249204808625544 2.4023908593832582E-4 1.2011954296916291E-4 1.2011954296916291E-4 2.4023908593832582E-4 28-29 0.009369324351594707 2.4023908593832582E-4 2.4023908593832582E-4 1.2011954296916291E-4 2.4023908593832582E-4 30-31 0.009669623209017614 2.4023908593832582E-4 2.4023908593832582E-4 1.2011954296916291E-4 2.4023908593832582E-4 32-33 0.01027022092386343 2.4023908593832582E-4 2.4023908593832582E-4 1.2011954296916291E-4 2.4023908593832582E-4 34-35 0.010870818638709243 2.4023908593832582E-4 2.4023908593832582E-4 1.2011954296916291E-4 2.4023908593832582E-4 36-37 0.011711655439493384 2.4023908593832582E-4 2.4023908593832582E-4 1.2011954296916291E-4 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