##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-30B_S30_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1163891
Sequences flagged as poor quality	0
Sequence length	61
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.47099513614248	40.0	40.0	40.0	40.0	40.0
2	39.22516283741347	40.0	40.0	40.0	40.0	40.0
3	39.18987430953586	40.0	40.0	40.0	40.0	40.0
4	39.04888688030065	40.0	40.0	40.0	40.0	40.0
5	39.103441817146106	40.0	40.0	40.0	40.0	40.0
6	39.18108482667191	40.0	40.0	40.0	40.0	40.0
7	39.21883750282458	40.0	40.0	40.0	40.0	40.0
8	39.23982572251182	40.0	40.0	40.0	40.0	40.0
9	39.23410010043896	40.0	40.0	40.0	40.0	40.0
10	39.24363363923254	40.0	40.0	40.0	40.0	40.0
11	39.230470894611265	40.0	40.0	40.0	40.0	40.0
12	39.24472652507838	40.0	40.0	40.0	40.0	40.0
13	39.0652286167691	40.0	40.0	40.0	40.0	40.0
14	39.23275289524535	40.0	40.0	40.0	40.0	40.0
15	39.14166876451489	40.0	40.0	40.0	40.0	40.0
16	39.324283803208374	40.0	40.0	40.0	40.0	40.0
17	39.272811629267686	40.0	40.0	40.0	40.0	40.0
18	39.36041433433199	40.0	40.0	40.0	40.0	40.0
19	39.31309375190632	40.0	40.0	40.0	40.0	40.0
20	39.32194337785927	40.0	40.0	40.0	40.0	40.0
21	39.29986914582207	40.0	40.0	40.0	40.0	40.0
22	39.36421194080889	40.0	40.0	40.0	40.0	40.0
23	39.351619696346134	40.0	40.0	40.0	40.0	40.0
24	39.359696054011934	40.0	40.0	40.0	40.0	40.0
25	39.308024548690554	40.0	40.0	40.0	40.0	40.0
26	39.27946861003307	40.0	40.0	40.0	40.0	40.0
27	39.29457311724208	40.0	40.0	40.0	40.0	40.0
28	39.31015876916309	40.0	40.0	40.0	40.0	40.0
29	39.3199191333209	40.0	40.0	40.0	40.0	40.0
30	39.31416945401244	40.0	40.0	40.0	40.0	40.0
31	39.26299627714279	40.0	40.0	40.0	40.0	40.0
32	39.29146286035376	40.0	40.0	40.0	40.0	40.0
33	39.28700367989786	40.0	40.0	40.0	40.0	40.0
34	39.29355583985098	40.0	40.0	40.0	40.0	40.0
35	39.29146286035376	40.0	40.0	40.0	40.0	40.0
36	39.258067980592685	40.0	40.0	40.0	40.0	40.0
37	39.274313488118736	40.0	40.0	40.0	40.0	40.0
38	39.263274653726164	40.0	40.0	40.0	40.0	40.0
39	38.028873837842205	40.0	40.0	40.0	40.0	40.0
40	39.26800705564353	40.0	40.0	40.0	40.0	40.0
41	39.26851569433907	40.0	40.0	40.0	40.0	40.0
42	39.23792176415145	40.0	40.0	40.0	40.0	40.0
43	39.26798299840792	40.0	40.0	40.0	40.0	40.0
44	39.23357771475164	40.0	40.0	40.0	40.0	40.0
45	39.093392766161095	40.0	40.0	40.0	40.0	40.0
46	39.100238768063335	40.0	40.0	40.0	40.0	40.0
47	39.19122666985139	40.0	40.0	40.0	40.0	40.0
48	39.26762213987392	40.0	40.0	40.0	40.0	40.0
49	39.262807256005935	40.0	40.0	40.0	40.0	40.0
50	39.24851382131145	40.0	40.0	40.0	40.0	40.0
51	39.21436973049882	40.0	40.0	40.0	40.0	40.0
52	39.221102319718945	40.0	40.0	40.0	40.0	40.0
53	39.16107264340046	40.0	40.0	40.0	40.0	40.0
54	39.082068681689265	40.0	40.0	40.0	40.0	40.0
55	39.13286725303315	40.0	40.0	40.0	40.0	40.0
56	39.12855069761687	40.0	40.0	40.0	40.0	40.0
57	39.06521830652527	40.0	40.0	40.0	40.0	40.0
58	39.042680113515786	40.0	40.0	40.0	40.0	40.0
59	39.00695511864943	40.0	40.0	40.0	40.0	40.0
60	38.965638534879986	40.0	40.0	40.0	40.0	40.0
61	38.87059183377138	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.09368135684967172
1101	2	-0.13333565644533962
1101	3	0.08072086667544198
1101	4	-0.029845812244538195
1101	5	-0.04890897443402764
1101	6	0.08749442789135031
1101	7	-0.1131754604448929
1101	8	0.04338127183088858
1101	9	-0.055649784125265
1101	10	-0.07567544544386351
1101	11	-0.03734025142584585
1101	12	-0.11952591779262178
1101	13	-0.1156024477164479
1101	14	-0.08931030480080437
1101	15	0.05953770989777496
1101	16	-0.013664625115502815
1101	17	-0.018174220891161497
1101	18	0.008112413092014492
1101	19	-0.07450860070252929
1101	20	-0.032521099607009774
1101	21	-0.03725009269463442
1101	22	-0.07741463344238042
1101	23	0.08644383899702746
1101	24	0.03826249468472298
1101	25	0.07382319173522944
1101	26	-0.02823923808085027
1101	27	-0.058393999831508836
1101	28	-0.02975776397106955
1101	29	-0.026051220991014645
1101	30	0.02212447715711363
1101	31	0.02220061380725724
1101	32	0.0032160906929661337
1101	33	7.03887491390276E-4
1101	34	0.019792121642232985
1101	35	0.09467586501686753
1101	36	-0.08372909999255995
1101	37	-0.11181107030194681
1101	38	-0.05543018203233885
1101	39	1.1890453073423615
1101	40	0.010246931957837546
1101	41	-0.04076734739057031
1101	42	-0.03051805176558986
1101	43	-0.024600070168247612
1101	44	-0.07943648256767943
1101	45	-0.0637363147169907
1101	46	-0.048529466231144625
1101	47	0.04951271892639397
1101	48	0.1289354861061014
1101	49	-0.02239919257645795
1101	50	0.007343081984188871
1101	51	0.12685084911268518
1101	52	0.017720297656381945
1101	53	-0.020883584821021373
1101	54	0.010013963701574369
1101	55	0.05665021117361135
1101	56	0.030540514840573962
1101	57	0.07865092219595482
1101	58	-0.05596309007189859
1101	59	0.04007639268354524
1101	60	0.10727146020522582
1101	61	0.006438690771183531
1102	1	-0.03363802103779534
1102	2	0.004188741732924939
1102	3	0.10620824933182149
1102	4	-0.021738646151035823
1102	5	-0.1464470820648458
1102	6	-0.059528994359787646
1102	7	-0.03171633320258849
1102	8	-0.1090161396385767
1102	9	-0.14772792800481227
1102	10	-0.015737835071149675
1102	11	-0.05824832838467131
1102	12	0.04065915981682622
1102	13	-0.12046872246317264
1102	14	-0.13397581739491216
1102	15	-0.1985636471056651
1102	16	-0.007562756912136592
1102	17	-0.07248398480974316
1102	18	-0.03103678757719308
1102	19	-0.03609947810749503
1102	20	-0.0771000567591571
1102	21	-0.07537398896752023
1102	22	-0.037904107371183215
1102	23	-0.07988260862821761
1102	24	-0.034287231126228335
1102	25	-0.0472626433795611
1102	26	-0.09871976601095866
1102	27	0.010199735645770147
1102	28	-0.12716865252948395
1102	29	-0.04799680143529628
1102	30	-0.08605123619914679
1102	31	-0.08383909713242588
1102	32	-0.12222888560594214
1102	33	0.04561454368620588
1102	34	-0.012921769053932053
1102	35	-0.06952329382689015
1102	36	-0.015165571783619214
1102	37	0.030066079061377593
1102	38	-0.020268340783154315
1102	39	1.1390209091640955
1102	40	-0.03218324277959539
1102	41	-0.04010046385120347
1102	42	-0.03201795882014835
1102	43	-0.0918484203866683
1102	44	-0.05723994943262767
1102	45	-0.10629312761781762
1102	46	0.06093412239233231
1102	47	0.02646980136899657
1102	48	-0.08055250069564579
1102	49	-0.03034021869412129
1102	50	0.001567684642424183
1102	51	-0.09776110367103286
1102	52	-0.13147804791476148
1102	53	-0.007322844975313103
1102	54	-0.10907238261314234
1102	55	-0.08717646881523677
1102	56	-0.008721382175878034
1102	57	-0.016681473937516955
1102	58	-0.08238457441058955
1102	59	-0.08119998058534605
1102	60	-0.0010425094945603064
1102	61	-0.08795728004394476
1103	1	0.023128470322411943
1103	2	0.04420062545374037
1103	3	-0.012892242202461546
1103	4	0.018630900159863018
1103	5	-0.0028290070118117683
1103	6	0.030247480908478508
1103	7	0.06597216800268768
1103	8	-0.04831823720162021
1103	9	-0.02571290358635281
1103	10	0.001333000393266559
1103	11	0.0030805909952178467
1103	12	-0.05601275093729896
1103	13	0.08080611591606868
1103	14	0.012613475602833546
1103	15	-0.04101457100740902
1103	16	0.04287025796337218
1103	17	-0.01869158880848687
1103	18	-0.026645565704818353
1103	19	-0.1230040062223452
1103	20	-0.07727989514918931
1103	21	0.0025668183054321503
1103	22	-0.013155467488928707
1103	23	0.06369086650408917
1103	24	-0.05987175984534332
1103	25	-0.007976155788767869
1103	26	0.05127724867176653
1103	27	-0.041700185009531765
1103	28	0.019455457180569624
1103	29	-0.02690075118989199
1103	30	0.07318083948835863
1103	31	0.0052879457417702724
1103	32	0.008691160403913045
1103	33	-0.06543551828610106
1103	34	0.12759819408639572
1103	35	-0.04606862712857662
1103	36	0.03063250423335262
1103	37	-6.218315546249187E-4
1103	38	-0.005492243175510225
1103	39	1.2679975495959397
1103	40	-0.025840846310757115
1103	41	-0.022965749790650136
1103	42	-0.005062629956874787
1103	43	-0.05072786895900805
1103	44	0.031823214845424275
1103	45	-0.004755509383066681
1103	46	0.021146676982276347
1103	47	-0.04387894843048201
1103	48	0.025654873109957066
1103	49	0.017518373660443842
1103	50	0.030019268910741914
1103	51	0.033691263973310015
1103	52	0.0771552019016184
1103	53	0.04038381990736184
1103	54	0.03875189657660627
1103	55	-0.045845866148020775
1103	56	-0.08232880360269235
1103	57	0.03206854134874959
1103	58	0.0015268674113286806
1103	59	-0.004260801209497345
1103	60	0.008067959473599728
1103	61	0.10642758993649437
1104	1	0.013248575040833543
1104	2	0.09835526414940432
1104	3	0.1162509716310609
1104	4	-0.03140200257894321
1104	5	-0.028833188662588327
1104	6	-0.06910856446028646
1104	7	-0.033671929358305874
1104	8	-0.028983323327004484
1104	9	-0.031049066742454556
1104	10	0.008271020678172647
1104	11	0.007800898991931149
1104	12	0.032352094563854905
1104	13	0.05003121716994485
1104	14	-0.027013260864592326
1104	15	-0.008640407600410072
1104	16	-0.03851249529805756
1104	17	-0.011824429313662677
1104	18	0.0686276307260485
1104	19	-0.0811212465517599
1104	20	-0.009899349230174437
1104	21	-0.028701513503385456
1104	22	0.012102947553948695
1104	23	-0.06660887607824861
1104	24	-0.0770851707971616
1104	25	0.008068916282560679
1104	26	0.01157504258237907
1104	27	0.034279577033593966
1104	28	-0.06218711897786022
1104	29	0.02676736769089416
1104	30	-0.012412945847515289
1104	31	-0.13436225256042889
1104	32	0.001002764679235213
1104	33	0.04815548043480078
1104	34	-0.08277280103673945
1104	35	-0.015290847862530654
1104	36	-0.04187479104341918
1104	37	-0.13148385162580212
1104	38	-0.09122314125968956
1104	39	1.174805816860946
1104	40	-0.07724574068857493
1104	41	-0.01933658274051453
1104	42	-0.11057578665960932
1104	43	0.058380464658782216
1104	44	-0.09048682962836097
1104	45	-0.011296305970688536
1104	46	-0.01228290892426287
1104	47	-0.03911860120685162
1104	48	0.008158018997775685
1104	49	-0.11748381769508853
1104	50	0.0579960306043219
1104	51	-0.06417209597170626
1104	52	-0.06878201918303972
1104	53	-0.016854703417145345
1104	54	-0.011958792499925153
1104	55	0.033770840349035325
1104	56	0.010846798220740084
1104	57	0.037353143198245675
1104	58	-0.03601466068432302
1104	59	-0.049995392120223414
1104	60	0.03885956523527767
1104	61	0.06401366239222739
1201	1	0.11277496327105041
1201	2	0.020661634797590978
1201	3	0.03185164387314643
1201	4	0.13114363391305517
1201	5	0.014479104223028116
1201	6	0.00593998562349185
1201	7	0.02885239335720513
1201	8	0.016469735243923367
1201	9	-0.013943770965767044
1201	10	0.0148602487571452
1201	11	-0.029155399845109287
1201	12	0.004347288475948119
1201	13	-0.035659258975130115
1201	14	-0.007820593859861447
1201	15	0.08191483167937008
1201	16	0.04654898240527672
1201	17	0.054516130216242686
1201	18	0.061246873824408965
1201	19	0.056835362237173115
1201	20	0.01779125101300849
1201	21	-0.10241842929284672
1201	22	0.07062473960689175
1201	23	-0.1140654003015058
1201	24	-0.01368952094921383
1201	25	-0.02084095519375495
1201	26	-0.02292951186214509
1201	27	0.007647184523314365
1201	28	0.11837826671568052
1201	29	0.016515045142021734
1201	30	0.06181561792812573
1201	31	0.06264070481774553
1201	32	0.05455769870113869
1201	33	-0.08377400895723497
1201	34	-0.015701441544912598
1201	35	-0.02477276467073608
1201	36	0.0633533624456959
1201	37	0.10917552884887272
1201	38	0.0028552520717539664
1201	39	1.2830264069665134
1201	40	0.08000416312072645
1201	41	0.07851052047082874
1201	42	0.09728384811349855
1201	43	0.02307381985058754
1201	44	-0.020518087389078232
1201	45	0.046316606804040816
1201	46	0.1289137459423273
1201	47	0.04500818011806018
1201	48	0.13224967146381772
1201	49	0.1297862073166911
1201	50	0.08357549615220705
1201	51	-0.012757927635121291
1201	52	0.021749456629493125
1201	53	0.045471590404631
1201	54	0.09431113965830917
1201	55	-0.024445253767204633
1201	56	0.05135676222788277
1201	57	0.08306173238612757
1201	58	0.045860458774775736
1201	59	0.07706111960521866
1201	60	-0.05539221339423506
1201	61	0.09329670831718317
1202	1	0.03483519133872903
1202	2	-0.0020225016358068615
1202	3	-0.0019357525999197378
1202	4	-0.02600534850446934
1202	5	0.02297469045236511
1202	6	-0.03241131675386555
1202	7	-0.014243974690202776
1202	8	0.039014518011363464
1202	9	-0.03428705918714314
1202	10	0.04496826838460066
1202	11	0.02507019203024896
1202	12	0.053938710861750394
1202	13	-0.020125285798215486
1202	14	-0.0723451550901757
1202	15	-0.08203490301325189
1202	16	-0.0687337581151155
1202	17	-0.02153840664772133
1202	18	-0.018798165280429657
1202	19	0.010621872714295932
1202	20	-0.029327303898277535
1202	21	-0.03841549083744411
1202	22	-0.006245676299258207
1202	23	0.048879521115495095
1202	24	-0.054419223684377016
1202	25	-0.04265963201749656
1202	26	0.09144552288459096
1202	27	0.034279577033593966
1202	28	0.002572729915826244
1202	29	-0.07361039809431702
1202	30	0.0858061583079035
1202	31	0.025378708044001996
1202	32	0.08950789150060956
1202	33	-0.05546027779509899
1202	34	-0.0158542905132677
1202	35	0.05594498592052588
1202	36	-0.1746324812754736
1202	37	-0.14443582140453515
1202	38	-0.18728358378532306
1202	39	1.1467432156736805
1202	40	-0.09667369535667802
1202	41	-0.03444721414903995
1202	42	-0.11381377910429791
1202	43	-0.09812250350096008
1202	44	-0.1239460848901004
1202	45	-0.09764277116227049
1202	46	-0.12777130611799947
1202	47	-0.05207057098558465
1202	48	-0.04472919093206684
1202	49	-0.05812062287587594
1202	50	-0.045619727625577866
1202	51	-0.039347487229129285
1202	52	-0.10439993607456444
1202	53	0.019842544289275565
1202	54	-0.042180055316975995
1202	55	0.01002556242135455
1202	56	-0.013977810521843992
1202	57	-0.04251733710396621
1202	58	-0.01658670601621992
1202	59	-0.12231055671816904
1202	60	-0.11440541047978314
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2403	6	-0.07331624712085016
2403	7	-0.10578488793348129
2403	8	-0.11648401045302847
2403	9	-0.14550276822855324
2403	10	-0.04484415781956841
2403	11	0.04106423908493895
2403	12	-0.14433253249109868
2403	13	-0.07561810755930054
2403	14	-0.09850911661471429
2403	15	-6.354456143924381E-4
2403	16	0.09562409022707641
2403	17	-0.03403953188679054
2403	18	0.03259961006388323
2403	19	0.030440759026433284
2403	20	-0.05462552408086907
2403	21	-0.004627613343139103
2403	22	-0.0444335568491212
2403	23	-0.04743794773241916
2403	24	-0.11027811323779702
2403	25	-0.04800595925963336
2403	26	-0.07731232851315184
2403	27	-0.14795538067314595
2403	28	0.00687986798314455
2403	29	-0.15074914991997446
2403	30	-0.03909904825752619
2403	31	0.03643892814300642
2403	32	0.026433691925660696
2403	33	-0.12010928783185193
2403	34	0.024625557859181413
2403	35	0.00357152907076852
2403	36	-0.059526328355893554
2403	37	-0.1021385153429577
2403	38	-0.002266570769698717
2403	39	1.2504710110184192
2403	40	-0.05224798367760286
2403	41	-0.08383131707100944
2403	42	-0.020879891037459686
2403	43	-0.016073889877880276
2403	44	-0.05444952246737955
2403	45	0.013206570522321215
2403	46	-0.0693481446313271
2403	47	-0.05294709664875086
2403	48	-0.03798749235054544
2403	49	0.025439892668600805
2403	50	-0.06530650600283394
2403	51	4.5234977783792374E-4
2403	52	0.0479286010816935
2403	53	0.05680550568784781
2403	54	-0.24162738881118173
2403	55	-0.02856643060957964
2403	56	0.03760150089625114
2403	57	-0.07781142479458225
2403	58	-0.054378861890398866
2403	59	-0.010291715310749794
2403	60	0.0027632438720885943
2403	61	-0.08692609384316796
2404	1	0.0014128057797577753
2404	2	0.040448422589520305
2404	3	-0.03143181215043711
2404	4	-0.003074177400804956
2404	5	0.010451479636458316
2404	6	-0.01931900423556243
2404	7	-0.08156448390837312
2404	8	-0.039585741563236354
2404	9	0.02974788377631654
2404	10	0.05575979251567276
2404	11	0.044112204164541424
2404	12	0.042940240657081574
2404	13	0.056240788723179946
2404	14	0.11177203109736666
2404	15	-0.046191605196746366
2404	16	-0.09847036274256027
2404	17	-0.07246022355099058
2404	18	-0.059927508063111645
2404	19	-0.046296028269736667
2404	20	-0.11651701487251387
2404	21	0.039051322713362424
2404	22	-0.025087878431456545
2404	23	0.03467636013315456
2404	24	-0.03323579542642108
2404	25	0.00925945087838187
2404	26	-0.0747387156148136
2404	27	0.007113742165266501
2404	28	-0.014653152020841276
2404	29	0.020741549978872342
2404	30	0.06658514971339713
2404	31	-0.06201438096808687
2404	32	-0.0247275304716581
2404	33	-0.053466935155135786
2404	34	-0.003378653507382978
2404	35	-0.04205527870879422
2404	36	0.051036265258701974
2404	37	0.05814297643501476
2404	38	0.03505678006512625
2404	39	1.2195656360210023
2404	40	-0.03549793837559179
2404	41	-0.037064963045551735
2404	42	0.03775994766444768
2404	43	-0.04144485025754108
2404	44	0.05067774750737186
2404	45	0.007526271964877651
2404	46	-0.03020812586826338
2404	47	7.363581398465158E-4
2404	48	0.0679939785540924
2404	49	0.010683594542385322
2404	50	0.05293655590838142
2404	51	0.038221610013223994
2404	52	-0.06452773122173738
2404	53	-0.02233699034966463
2404	54	-0.08867037181661885
2404	55	0.011064455497724168
2404	56	-0.08960470697001455
2404	57	-0.052757005038778004
2404	58	-0.06339338835250885
2404	59	0.04356147865295412
2404	60	-0.07885469159491265
2404	61	-0.028429386553789016
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	7.0
17	43.0
18	116.0
19	187.0
20	253.0
21	288.0
22	430.0
23	601.0
24	940.0
25	1096.0
26	1501.0
27	1907.0
28	2597.0
29	3158.0
30	4032.0
31	4966.0
32	6843.0
33	8183.0
34	10511.0
35	13655.0
36	20065.0
37	29256.0
38	73256.0
39	484486.0
40	495514.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.17731041824363	14.736173748229001	24.744928863613517	30.34158696991385
2	32.006356562230884	16.119935849945254	27.73226462413259	24.141442963691276
3	28.939590511130774	19.24709379752382	21.750423149954035	30.062892541391367
4	26.428894851656814	21.006556467154514	21.116876265692003	31.44767241549667
5	25.653003588824042	21.653230414188272	22.23103366208691	30.462732334900778
6	25.954148627319913	24.101999242197078	24.658924246342657	25.28492788414035
7	25.49628788262818	23.764854268999418	23.77653921200525	26.96231863636715
8	24.181731794472164	25.290168924753264	24.35348327291817	26.174616007856404
9	24.057493356336632	25.292230973518997	25.067295820656742	25.582979849487625
10	25.187152405165087	23.710209976707443	24.306227988703412	26.796409629424062
11	24.679974327492868	24.40615143514298	24.203555143909526	26.710319093454626
12	25.146856535534685	24.11033335595859	24.37307273619265	26.369737372314074
13	24.627392083966626	22.75866039001934	24.36035676880395	28.253590757210084
14	24.726112668626186	23.742429488672048	24.261464346747246	27.26999349595452
15	24.861692374973256	23.693799505280133	24.139030201281734	27.30547791846487
16	25.171429283326358	23.904558072877958	23.633828253676676	27.290184390119006
17	25.26439331518158	23.462248612627814	23.75368483818502	27.519673234005587
18	25.534349866095706	23.971918332558634	23.96805199112288	26.52567981022278
19	25.238531786911317	23.328387280252187	24.044004120660784	27.389076812175713
20	25.124775430001606	23.728081066010475	23.64405257880678	27.50309092518114
21	24.989195723654532	23.90464399157653	24.293855696109	26.812304588659934
22	25.775867327782414	23.17528015939637	23.59911709945347	27.449735413367748
23	25.247123656768544	24.036271437789278	23.620339018000827	27.09626588744135
24	25.355209379572486	24.546972182102962	23.645341359285364	26.452477079039188
25	25.36216879415684	23.50031059609534	23.39497427164571	27.74254633810211
26	25.539504988010048	23.848195406614536	23.90490174767225	26.707397857703167
27	25.377977834694143	24.494475857275294	23.485790336036622	26.64175597199394
28	25.507113638648292	24.054743957982318	23.258019866121487	27.180122537247904
29	25.55015890663301	25.049166975257993	23.222019931419695	26.178654186689304
30	26.002349017218968	25.195572437625174	23.18507489103361	25.61700365412225
31	25.727237344390495	24.52386005218702	23.559164904617358	26.189737698805125
32	25.84417269314738	24.9229524070553	23.020626501966248	26.21224839783107
33	25.802224956353186	25.982053255983395	23.000580811899425	25.21514097576399
34	26.347312591986704	25.1037253488514	22.620245366619383	25.928716692542515
35	26.369479616218356	26.290348494833278	22.288942864924636	25.051229024023726
36	26.593641500793456	26.996428359700346	22.032905143179214	24.377024996326977
37	26.692877597644454	26.344649112330963	21.66293922712694	25.299534062897642
38	26.806633954554165	27.36991694239409	21.405354968807217	24.41809413424453
39	27.329113778115953	27.265261744561712	20.98541424450185	24.420210232820477
40	27.70242230586885	27.095406700455626	20.62925136460373	24.572919629071794
41	27.180895805535055	27.67389729794285	20.55115126760152	24.59405562892058
42	27.243616455492827	27.916531702710994	20.937355817683958	23.902496024112224
43	27.370862048078386	28.474058137746578	19.843009353968714	24.312070460206325
44	27.415367933938832	28.40171459354871	20.010550816184676	24.172366656327785
45	27.430919218380417	28.727174623740538	19.50990255960395	24.332003598275094
46	27.647520257481155	28.6618764128256	19.489024315850884	24.201579013842363
47	28.087509912869844	28.77898359897963	18.850820222855923	24.282686265294604
48	27.947977946388452	29.295698652193376	18.749607995937765	24.00671540548041
49	28.026593555582096	29.04936974338662	18.46719323373065	24.456843467300633
50	28.003309588269005	29.245608050925732	18.23641560936548	24.51466675143978
51	27.713162143190385	29.554228016197392	18.26854920263152	24.464060637980705
52	27.59055616032773	29.518829512385608	17.83208221388429	25.05853211340237
53	27.423616129001772	29.718332730470465	17.79341879952676	25.064632341001
54	27.156752651236243	29.771430486188137	17.868425823380367	25.20339103919525
55	27.112246765375797	29.508003756365504	17.70371967821729	25.676029800041412
56	26.657221337736953	29.92582638752254	17.717638507386003	25.699313767354504
57	26.281327031483187	29.91302450143527	17.709562149720206	26.096086317361333
58	26.078387065455445	29.90237058281231	17.447166444280434	26.572075907451815
59	25.72732326308907	30.09912440254285	17.4640065092006	26.709545825167474
60	25.33957217643233	30.284708791458993	17.26390186022574	27.111817171882933
61	25.37411149325839	30.049119719973778	17.140780365171654	27.43598842159618
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	7.0
2	7.0
3	4.0
4	4.0
5	4.0
6	6.5
7	7.0
8	5.0
9	7.0
10	9.0
11	11.5
12	22.0
13	30.0
14	42.0
15	54.0
16	57.5
17	89.0
18	117.0
19	176.5
20	274.5
21	313.0
22	421.5
23	530.0
24	763.0
25	1329.5
26	1663.0
27	2148.0
28	2633.0
29	3581.5
30	5655.0
31	6780.0
32	7967.5
33	9155.0
34	11068.0
35	15065.5
36	17150.0
37	19842.0
38	22534.0
39	26157.5
40	33436.5
41	37092.0
42	41661.0
43	49821.5
44	53413.0
45	57161.0
46	60909.0
47	64557.0
48	70185.0
49	72165.0
50	74906.5
51	77648.0
52	79330.5
53	81589.5
54	82166.0
55	81458.5
56	80751.0
57	78438.0
58	72401.0
59	68677.0
60	64296.5
61	56384.5
62	52853.0
63	45658.0
64	38463.0
65	33681.5
66	25329.0
67	21758.0
68	18785.0
69	15812.0
70	13393.5
71	9312.0
72	7649.0
73	6242.0
74	4835.0
75	4059.0
76	2668.5
77	2054.0
78	1691.5
79	1329.0
80	1074.0
81	678.5
82	538.0
83	412.5
84	227.5
85	168.0
86	134.5
87	101.0
88	79.5
89	45.5
90	33.0
91	27.5
92	22.0
93	14.0
94	8.5
95	11.0
96	9.0
97	7.0
98	9.5
99	8.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03170399977317464
3	8.591869857228899E-5
4	1.7183739714457798E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	2.5775609571686693E-4
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.1174740589969336
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1163891.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.28216437923018
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.06746164659062	60.27651809106344
2	14.424496931143995	21.718146981161677
3	3.3033920685244444	7.460595141351075
4	1.002350794403064	3.0183654907961373
5	0.4257911174688603	1.6027238448253422
6	0.24674064509675758	1.1145101881926842
7	0.15058007681462607	0.7935195866497053
8	0.10120542600165444	0.6095170813061255
9	0.06777441756252536	0.45919843592837584
>10	0.20695310470106024	2.3268965552249323
>50	0.0025029013018395144	0.1242655140582322
>100	6.257253254598786E-4	0.059918777059162764
>500	0.0	0.0
>1k	1.251450650919757E-4	0.43582431238315555
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACACGCTATCTGGGGGGGGGGGGGGG	4626	0.39745989959540884	TruSeq Adapter, Index 1 (97% over 39bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.4756459152961918	0.0	0.0	0.0	0.0
2	0.4770206144733484	0.0	0.0	0.0	0.0
3	0.47848123234907736	0.0	0.0	0.0	1.7183739714457798E-4
4	0.4805432811148123	0.0	0.0	0.0	1.7183739714457798E-4
5	0.4819179802919689	0.0	0.0	0.0	1.7183739714457798E-4
6	0.4855265656320051	0.0	0.0	0.0	1.7183739714457798E-4
7	0.48939290706775807	0.0	0.0	0.0	1.7183739714457798E-4
8	0.5192066954723423	0.0	0.0	0.0	1.7183739714457798E-4
9	0.5230730369080954	0.0	0.0	0.0	1.7183739714457798E-4
10	0.5277126466309989	0.0	0.0	0.0	1.7183739714457798E-4
11	0.5328677685453362	0.0	0.0	0.0	1.7183739714457798E-4
12	0.5446386302497399	0.0	0.0	0.0	1.7183739714457798E-4
13	0.5631970691413543	0.0	0.0	0.0	1.7183739714457798E-4
14	0.5865669551530169	0.0	0.0	0.0	1.7183739714457798E-4
15	0.6132876704089988	0.0	0.0	0.0	1.7183739714457798E-4
16	0.6496312799050771	0.0	0.0	0.0	1.7183739714457798E-4
17	0.6879510194683179	0.0	0.0	0.0	1.7183739714457798E-4
18	0.7362373280659443	0.0	0.0	0.0	1.7183739714457798E-4
19	0.8025665633637514	0.0	0.0	0.0	1.7183739714457798E-4
20	0.8948432456303897	0.0	0.0	0.0	2.5775609571686693E-4
21	1.0348907243032208	0.0	0.0	0.0	2.5775609571686693E-4
22	1.2150622352093108	0.0	0.0	0.0	2.5775609571686693E-4
23	1.4545176481302802	0.0	0.0	0.0	2.5775609571686693E-4
24	1.7586698410761832	0.0	0.0	0.0	2.5775609571686693E-4
25	2.1726261307974717	0.0	0.0	0.0	3.4367479428915596E-4
26	2.6818662572354284	0.0	0.0	0.0	3.4367479428915596E-4
27	3.3057219275688188	0.0	0.0	0.0	3.4367479428915596E-4
28	4.0386943450890165	0.0	0.0	0.0	3.4367479428915596E-4
29	4.901747672247659	0.0	0.0	0.0	3.4367479428915596E-4
30	5.902786429313398	0.0	0.0	0.0	3.4367479428915596E-4
31	7.000827397067251	0.0	0.0	0.0	3.4367479428915596E-4
32	8.212538803032242	0.0	0.0	0.0	3.4367479428915596E-4
33	9.553729687745674	0.0	0.0	0.0	3.4367479428915596E-4
34	11.0115122464217	0.0	0.0	0.0	3.4367479428915596E-4
35	12.567328040168709	0.0	0.0	0.0	3.4367479428915596E-4
36	14.225558922613887	0.0	0.0	0.0	3.4367479428915596E-4
37	15.984744275881503	0.0	0.0	0.0	3.4367479428915596E-4
38	17.851070246268765	0.0	0.0	0.0	3.4367479428915596E-4
39	19.772126427646576	8.591869857228899E-5	0.0	0.0	3.4367479428915596E-4
40	21.786404396975318	8.591869857228899E-5	0.0	0.0	3.4367479428915596E-4
41	23.91022870698373	8.591869857228899E-5	0.0	0.0	3.4367479428915596E-4
42	26.05501717944378	8.591869857228899E-5	0.0	0.0	3.4367479428915596E-4
43	28.26587713110592	8.591869857228899E-5	0.0	0.0	3.4367479428915596E-4
44	30.516689277604176	8.591869857228899E-5	0.0	0.0	3.4367479428915596E-4
45	32.780818822381136	8.591869857228899E-5	0.0	0.0	3.4367479428915596E-4
46	35.07029438323692	8.591869857228899E-5	0.0	0.0	3.4367479428915596E-4
47	37.28235719667907	8.591869857228899E-5	0.0	0.0	3.4367479428915596E-4
48	39.556367391791845	8.591869857228899E-5	0.0	0.0	3.4367479428915596E-4
49	41.78441108316844	8.591869857228899E-5	0.0	0.0	3.4367479428915596E-4
50	44.004292498180675	8.591869857228899E-5	0.0	0.0	3.4367479428915596E-4
>>END_MODULE