##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-30B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 662342 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.56917121366303 40.0 40.0 40.0 40.0 40.0 2 39.36774959160071 40.0 40.0 40.0 40.0 40.0 3 39.33975801021225 40.0 40.0 40.0 40.0 40.0 4 39.21333691657784 40.0 40.0 40.0 40.0 40.0 5 39.262417301031796 40.0 40.0 40.0 40.0 40.0 6 39.343595906646414 40.0 40.0 40.0 40.0 40.0 7 39.378985478800985 40.0 40.0 40.0 40.0 40.0 8 39.406191967291825 40.0 40.0 40.0 40.0 40.0 9 39.39822025479284 40.0 40.0 40.0 40.0 40.0 10-11 39.40412354946538 40.0 40.0 40.0 40.0 40.0 12-13 39.31987402278581 40.0 40.0 40.0 40.0 40.0 14-15 39.35467779485523 40.0 40.0 40.0 40.0 40.0 16-17 39.46181579908869 40.0 40.0 40.0 40.0 40.0 18-19 39.49876951786237 40.0 40.0 40.0 40.0 40.0 20-21 39.4777169498537 40.0 40.0 40.0 40.0 40.0 22-23 39.51781707939403 40.0 40.0 40.0 40.0 40.0 24-25 39.494922562664 40.0 40.0 40.0 40.0 40.0 26-27 39.46734466484082 40.0 40.0 40.0 40.0 40.0 28-29 39.49382041301925 40.0 40.0 40.0 40.0 40.0 30-31 39.47324521535589 40.0 40.0 40.0 40.0 40.0 32-33 39.47538346696699 40.0 40.0 40.0 40.0 40.0 34-35 39.47759305267189 40.0 40.0 40.0 40.0 40.0 36-37 39.45698511947312 40.0 40.0 40.0 40.0 40.0 38-39 39.39365650003462 40.0 40.0 40.0 40.0 40.0 40-41 39.44726467731376 40.0 40.0 40.0 40.0 40.0 42-43 39.43158192905029 40.0 40.0 40.0 40.0 40.0 44-45 39.3254993355183 40.0 40.0 40.0 40.0 40.0 46-47 39.3209965042645 40.0 40.0 40.0 40.0 40.0 48-49 39.4100752049423 40.0 40.0 40.0 40.0 40.0 50-51 39.37185003310077 40.0 40.0 40.0 40.0 40.0 52-53 39.335674306231766 40.0 40.0 40.0 40.0 40.0 54-55 39.26277808106589 40.0 40.0 40.0 40.0 40.0 56-57 39.27096092381478 40.0 40.0 40.0 40.0 40.0 58-59 39.216373699938174 40.0 40.0 40.0 40.0 40.0 60-61 39.12684449356533 40.0 40.0 40.0 40.0 40.0 62-63 39.39809504690836 40.0 40.0 40.0 40.0 40.0 64-65 39.41078703132297 40.0 40.0 40.0 40.0 40.0 66-67 39.42426173035575 40.0 40.0 40.0 40.0 40.0 68-69 39.42602258854934 40.0 40.0 40.0 40.0 40.0 70-71 39.419606943382874 40.0 40.0 40.0 40.0 40.0 72-73 39.41606138117224 40.0 40.0 40.0 40.0 40.0 74-75 39.41415865192812 40.0 40.0 40.0 40.0 40.0 76-77 39.43019871526067 40.0 40.0 40.0 40.0 40.0 78-79 39.42132376769962 40.0 40.0 40.0 40.0 40.0 80-81 39.40724749922127 40.0 40.0 40.0 40.0 40.0 82-83 39.408009541046056 40.0 40.0 40.0 40.0 40.0 84-85 39.39948651076837 40.0 40.0 40.0 40.0 40.0 86-87 39.39866427658431 40.0 40.0 40.0 40.0 40.0 88-89 39.47321614543179 40.0 40.0 40.0 40.0 40.0 90-91 39.51305488868675 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0013515296411981126 1101 2 0.004607629054021345 1101 3 -0.02872335894142708 1101 4 -0.13124322665380816 1101 5 0.04134538213882877 1101 6 -0.08103697218655981 1101 7 -0.08934983458286894 1101 8 -0.06308030487461025 1101 9 -0.02367947651300284 1101 10-11 -0.04085448827085969 1101 12-13 -0.05322172048021656 1101 14-15 -0.02005688466887534 1101 16-17 -0.026626913009188513 1101 18-19 -0.008908201873182975 1101 20-21 -0.020224164708565695 1101 22-23 -0.003916100815892776 1101 24-25 -0.0367193069545948 1101 26-27 -0.06982878234806122 1101 28-29 0.02587021488348995 1101 30-31 0.06829703116937935 1101 32-33 0.0077198621451159966 1101 34-35 0.020792760495631057 1101 36-37 -0.07444747033379429 1101 38-39 -0.005876966934131644 1101 40-41 -0.12234433782867882 1101 42-43 0.027002376381339843 1101 44-45 -0.13624968415994942 1101 46-47 -0.0494879665979866 1101 48-49 -0.15721110974273955 1101 50-51 -0.05652676327327555 1101 52-53 -0.1122241582908714 1101 54-55 -0.21257121696307735 1101 56-57 0.07592080745207852 1101 58-59 -0.2752968025727469 1101 60-61 -0.3351336651058361 1101 62-63 -0.4734958356073662 1101 64-65 -0.23574741599106375 1101 66-67 -0.30054684284735345 1101 68-69 -0.23206887542295362 1101 70-71 -0.13598951351697508 1101 72-73 -0.3891069790821149 1101 74-75 -0.07163982749585074 1101 76-77 -0.06859330730556223 1101 78-79 -0.3023165763180131 1101 80-81 -0.5082519768318292 1101 82-83 -0.13468520026658126 1101 84-85 -0.5629116351960377 1101 86-87 -0.19509752199822117 1101 88-89 -0.03943960059798002 1101 90-91 0.3990442815828956 1102 1 0.020163458314712557 1102 2 -0.07228799984874712 1102 3 -0.05016734616481244 1102 4 0.06012200983818872 1102 5 -0.08627876296970527 1102 6 -0.09108176706057236 1102 7 -0.14239387168492357 1102 8 -0.34895409267927846 1102 9 -0.16046594788553392 1102 10-11 -0.06461207798050594 1102 12-13 -0.04746457200487697 1102 14-15 -0.007644771827997943 1102 16-17 -0.024537764495477177 1102 18-19 -0.034029781108316115 1102 20-21 -0.09614332291025107 1102 22-23 -0.07087052898582158 1102 24-25 -0.05837048246033305 1102 26-27 -0.006642111460898548 1102 28-29 -0.03433332841981951 1102 30-31 -0.12189413838929397 1102 32-33 -0.04398251966114941 1102 34-35 -0.09808729576199227 1102 36-37 -0.06676692771652881 1102 38-39 -0.05475435567427667 1102 40-41 -0.08827952140093487 1102 42-43 -0.08599320112363529 1102 44-45 -0.12328167707863003 1102 46-47 -0.04583904431792263 1102 48-49 -0.09662498300708933 1102 50-51 -0.07111451777114297 1102 52-53 0.03161698392810308 1102 54-55 -0.04954872155335721 1102 56-57 0.0595649002015648 1102 58-59 -0.14797446507940037 1102 60-61 -0.05480761211925511 1102 62-63 -0.028859593350276214 1102 64-65 0.13138774496386674 1102 66-67 -0.21660991723184253 1102 68-69 -0.0711589554129759 1102 70-71 0.13028078684885713 1102 72-73 -0.031655003266422455 1102 74-75 -0.15051357315589797 1102 76-77 -0.13656626896204926 1102 78-79 -0.23808105147873704 1102 80-81 -0.2767742014928132 1102 82-83 0.24806991607768936 1102 84-85 0.1269146675334909 1102 86-87 -0.09260197832621486 1102 88-89 0.46056039940202 1102 90-91 0.3990442815828956 1103 1 0.0075832965485034265 1103 2 0.10790541222694117 1103 3 0.015644882169404184 1103 4 -0.058263306480839105 1103 5 -0.0059392214624338635 1103 6 0.028777874224253708 1103 7 0.03696371727253478 1103 8 0.02701366576970088 1103 9 -0.009410930619921487 1103 10-11 0.06407862092770955 1103 12-13 -0.04475233529381484 1103 14-15 0.05322697865367587 1103 16-17 0.007727986302647594 1103 18-19 -0.016801400352719043 1103 20-21 -0.012966822756673935 1103 22-23 -0.013343932498507627 1103 24-25 -0.028924936615709385 1103 26-27 0.12299237880800007 1103 28-29 -5.355454918287705E-4 1103 30-31 -0.10275769817670266 1103 32-33 -0.08371794398812682 1103 34-35 -0.05089574974379474 1103 36-37 0.016188577986923747 1103 38-39 -0.040434513488683876 1103 40-41 0.0015439703973143537 1103 42-43 0.03091331190885427 1103 44-45 0.006624249478598188 1103 46-47 0.1374586890081062 1103 48-49 0.013198922376680855 1103 50-51 0.10048341587935994 1103 52-53 0.13688598155718523 1103 54-55 0.159856422669165 1103 56-57 0.0506487236947919 1103 58-59 -0.06601657041723286 1103 60-61 0.14784403397267454 1103 62-63 0.0050393609690644325 1103 64-65 0.025412569677776276 1103 66-67 -0.16779438778775813 1103 68-69 0.11847471823373468 1103 70-71 0.1497294104753948 1103 72-73 -0.10290737230677394 1103 74-75 0.07257594340657647 1103 76-77 -0.21749920071407303 1103 78-79 -0.22775441468151314 1103 80-81 -0.15867408872042432 1103 82-83 -0.6530870570305041 1103 84-85 -0.23400127687391148 1103 86-87 -0.0018769139169307891 1103 88-89 -0.055568632856044076 1103 90-91 0.3990442815828956 1104 1 -0.033895221846776735 1104 2 -0.031211886804783262 1104 3 0.020385284271725368 1104 4 -0.12586346586058283 1104 5 -0.17237772915202498 1104 6 0.02253771113127101 1104 7 -0.037362460247805984 1104 8 -0.03414017366063149 1104 9 -0.002487194792450964 1104 10-11 -0.03770801154826131 1104 12-13 0.005039970715294828 1104 14-15 0.009639270665815047 1104 16-17 -0.018324935729673086 1104 18-19 0.024098432129761704 1104 20-21 -0.052190877283351256 1104 22-23 -0.04160610856249747 1104 24-25 -0.07033473605885376 1104 26-27 0.026626104381882953 1104 28-29 -0.08478015843836317 1104 30-31 -0.009110644742897023 1104 32-33 -0.10406985535162505 1104 34-35 -0.03938934811809247 1104 36-37 -0.16752496307734077 1104 38-39 -0.06342973949033848 1104 40-41 -0.1123341200012149 1104 42-43 -0.08803850810182468 1104 44-45 -0.15443991935924117 1104 46-47 -0.23735389999336576 1104 48-49 -0.028492791927632766 1104 50-51 -0.22642735805134606 1104 52-53 -0.06350472323254763 1104 54-55 0.03651306399372345 1104 56-57 0.0336776638046814 1104 58-59 -0.09112298290725107 1104 60-61 -0.20661006213808264 1104 62-63 -0.3569934311435361 1104 64-65 -0.014843347958176878 1104 66-67 -0.1459598703574443 1104 68-69 -0.10276103922713986 1104 70-71 -0.031502633073031916 1104 72-73 -0.11398722477897394 1104 74-75 0.07172777462043456 1104 76-77 -0.011645443077640039 1104 78-79 -0.13320904593273752 1104 80-81 0.11514359936430907 1104 82-83 0.45574325123801174 1104 84-85 0.163959195123077 1104 86-87 -0.31078379650802646 1104 88-89 0.46056039940202 1104 90-91 -0.022008349996049503 1201 1 0.1382431772615078 1201 2 -0.027539994691125003 1201 3 0.01098165812697971 1201 4 -0.1871649422419921 1201 5 0.01542611871013122 1201 6 -0.06847293057847281 1201 7 -0.0424430809113403 1201 8 0.060065522492109835 1201 9 -0.029424892441056727 1201 10-11 -0.004175673119384271 1201 12-13 -0.12912448018918354 1201 14-15 -0.011868044677584066 1201 16-17 -0.04173234026834649 1201 18-19 0.006552258146946599 1201 20-21 0.013639346721433299 1201 22-23 -0.08034735393178494 1201 24-25 -0.022232223221145375 1201 26-27 -0.05393404675387359 1201 28-29 -0.03666796361749647 1201 30-31 -0.029094257986400862 1201 32-33 -0.16730819082786041 1201 34-35 0.03339881849618109 1201 36-37 -0.01005903219765969 1201 38-39 0.061968882148157434 1201 40-41 0.09153981134089406 1201 42-43 0.01948418671933183 1201 44-45 -0.07456043280227931 1201 46-47 -0.034723913603933454 1201 48-49 0.036330778656179064 1201 50-51 -0.021306765515802795 1201 52-53 0.0016923770194452459 1201 54-55 0.1029199261827003 1201 56-57 0.006802322095083468 1201 58-59 0.19074249947644262 1201 60-61 -0.06361214066855325 1201 62-63 -0.1593429221754974 1201 64-65 -0.19540471520520697 1201 66-67 -0.02671189723157852 1201 68-69 -0.003160838562138224 1201 70-71 -0.19886100159743592 1201 72-73 -0.21973124812020473 1201 74-75 -0.03278183978875404 1201 76-77 0.019810518032763014 1201 78-79 -0.23320784466286426 1201 80-81 -0.022081885511632038 1201 82-83 0.3643712910730059 1201 84-85 -0.0574171297015269 1201 86-87 0.13638601481272872 1201 88-89 0.46056039940202 1201 90-91 0.3990442815828956 1202 1 0.13328418385738416 1202 2 0.05943219045752812 1202 3 -0.014009154074415164 1202 4 -0.05470106479755543 1202 5 0.1641523996826848 1202 6 0.04516563109583416 1202 7 0.005019152400713267 1202 8 -0.022474758751236834 1202 9 0.053004168082352976 1202 10-11 -0.06660434678812521 1202 12-13 -0.016153242834491266 1202 14-15 -0.11467346644589327 1202 16-17 -0.056090693427982785 1202 18-19 -0.09050837165246861 1202 20-21 0.005454952439137628 1202 22-23 0.0774011034851867 1202 24-25 -0.009377767622389399 1202 26-27 -0.012280678216761487 1202 28-29 -0.02163261477129197 1202 30-31 2.4383365947500124E-4 1202 32-33 -0.028643454040484073 1202 34-35 -0.021003091946624863 1202 36-37 -0.2938604170385659 1202 38-39 -0.09372319172778276 1202 40-41 -0.14047431532172538 1202 42-43 -0.06933528614055717 1202 44-45 -0.07161482468465152 1202 46-47 -0.0781358975642874 1202 48-49 -0.055794671015263475 1202 50-51 -0.0699285506675622 1202 52-53 -0.29090788189132155 1202 54-55 -0.17238366292832552 1202 56-57 -0.19024160171058924 1202 58-59 -0.14316213714644732 1202 60-61 -0.01297669003638191 1202 62-63 -0.04132581449473349 1202 64-65 0.20153801430486595 1202 66-67 0.09503395647863044 1202 68-69 0.18695056502919982 1202 70-71 0.09371003772448461 1202 72-73 0.024954834118133817 1202 74-75 0.04643028607650734 1202 76-77 -0.5558737006290286 1202 78-79 -0.3078952929648082 1202 80-81 0.10385414194681175 1202 82-83 -0.5486445664648301 1202 84-85 -0.3184061406905343 1202 86-87 -0.24034639077650155 1202 88-89 -0.513798574956958 1202 90-91 0.3990442815828956 1203 1 0.028202610497231717 1203 2 -0.015618094490513101 1203 3 -0.09468855959458722 1203 4 -0.08774945009199797 1203 5 -0.14649683486865683 1203 6 -0.0032196633504710803 1203 7 -0.026520684452563614 1203 8 -0.06407158521229661 1203 9 -0.29033028269427064 1203 10-11 -0.11834197110368905 1203 12-13 0.03457868252029783 1203 14-15 0.07485189951677995 1203 16-17 -0.04562164660710977 1203 18-19 -0.03976946227712119 1203 20-21 -0.08110499839335716 1203 22-23 -0.1163705469785512 1203 24-25 -0.04021221839901301 1203 26-27 0.01710109628317724 1203 28-29 -0.037744750205419564 1203 30-31 -0.01710447341393717 1203 32-33 0.054018471385766986 1203 34-35 0.03531661459288671 1203 36-37 -0.01425504751897222 1203 38-39 0.014882922974258861 1203 40-41 0.06153123972401886 1203 42-43 0.0027125302286918895 1203 44-45 -0.11680927945957364 1203 46-47 -0.0013074873306848644 1203 48-49 0.03364863101782589 1203 50-51 0.007055057893730066 1203 52-53 -0.07628258085622974 1203 54-55 -0.009387371402517886 1203 56-57 -0.16038661558517475 1203 58-59 -0.22494656526991008 1203 60-61 0.11437806571694864 1203 62-63 -0.045641686201086884 1203 64-65 -0.0841626761279457 1203 66-67 -0.15871512370465268 1203 68-69 -0.3271089548815169 1203 70-71 -0.2784576875982907 1203 72-73 -0.22816911944762097 1203 74-75 -0.8676966387489458 1203 76-77 -0.34592554561100997 1203 78-79 0.02191483498774005 1203 80-81 6.289228731830576E-4 1203 82-83 0.5456308916874448 1203 84-85 0.35714869662900384 1203 86-87 0.35392208584491414 1203 88-89 0.16426410310572237 1203 90-91 0.3990442815828956 1204 1 0.11732676149056687 1204 2 0.15844954132553113 1204 3 -0.2001450018131976 1204 4 -0.11208161675366313 1204 5 -0.2521857216814851 1204 6 -0.09093023980194204 1204 7 -0.03488646549420338 1204 8 -0.08045726084198179 1204 9 -0.0858257511368592 1204 10-11 -0.17377444594906422 1204 12-13 -0.24407178197963475 1204 14-15 -0.10113590659550908 1204 16-17 -0.011769817245905756 1204 18-19 -0.06723851878562215 1204 20-21 0.03573511197890156 1204 22-23 -0.04762589237008541 1204 24-25 -0.03598706522470252 1204 26-27 -0.08077031180834382 1204 28-29 -0.020150140250784432 1204 30-31 -0.09699868331919248 1204 32-33 -0.06671867390850394 1204 34-35 -0.05615821744547134 1204 36-37 -0.09602233782783998 1204 38-39 0.01973719880613345 1204 40-41 -0.0650833654544769 1204 42-43 -0.15841452296621128 1204 44-45 -0.08466946472157844 1204 46-47 -0.07529995192604844 1204 48-49 -0.04390389779962334 1204 50-51 -0.00904868840739681 1204 52-53 -0.07516325684284197 1204 54-55 -0.10626274098321886 1204 56-57 -0.11475112823285372 1204 58-59 -0.06850787982202178 1204 60-61 -0.013015864889410977 1204 62-63 -0.08331901444110201 1204 64-65 -0.07032599885514657 1204 66-67 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34-35 -0.0028331344047032303 2401 36-37 0.09628159280174486 2401 38-39 0.003274122950720937 2401 40-41 0.033720824126397986 2401 42-43 0.14929678902778676 2401 44-45 0.10063189151188823 2401 46-47 0.038555322605340336 2401 48-49 0.07834563467747557 2401 50-51 0.04117981532534287 2401 52-53 0.11269251031103522 2401 54-55 0.12605809968361825 2401 56-57 0.13440237216876483 2401 58-59 0.09576431381951522 2401 60-61 0.12156903581349354 2401 62-63 0.045708059861766515 2401 64-65 0.13238810347385055 2401 66-67 0.12186140316589444 2401 68-69 -0.016988807175728482 2401 70-71 -0.05295916376945797 2401 72-73 0.05468023250593035 2401 74-75 -0.13663481766971586 2401 76-77 0.1672711911811149 2401 78-79 0.22024944835125382 2401 80-81 0.2731852787166247 2401 82-83 0.4772548233113767 2401 84-85 0.21926271219570737 2401 86-87 -0.28893505701222466 2401 88-89 -0.11086817202654942 2401 90-91 0.3990442815828956 2402 1 -0.04329585319176488 2402 2 -0.01640141529598793 2402 3 0.058040535366579604 2402 4 0.057062038613146626 2402 5 -0.010523420302057218 2402 6 -0.014919364000597568 2402 7 -0.029638143011730733 2402 8 0.02003913467798668 2402 9 -0.027076468291184597 2402 10-11 0.08212617516894483 2402 12-13 0.08055592138038747 2402 14-15 0.05836919455443024 2402 16-17 0.029987273227796152 2402 18-19 -0.05300019904704101 2402 20-21 0.023911419694229608 2402 22-23 0.06812655842688997 2402 24-25 -0.022507058240236688 2402 26-27 -0.0783057953574442 2402 28-29 0.04698049408781202 2402 30-31 0.024451090933098385 2402 32-33 0.06053843838079587 2402 34-35 0.031780754317196624 2402 36-37 -0.004154298406696455 2402 38-39 -0.41116330100766874 2402 40-41 -0.029994277706165917 2402 42-43 0.07074860843636088 2402 44-45 -0.03499500257681376 2402 46-47 0.04952646691033635 2402 48-49 0.08343786195655412 2402 50-51 0.06061159043210296 2402 52-53 0.048534846812302135 2402 54-55 -0.03691290705765482 2402 56-57 0.06206273205550872 2402 58-59 0.1813630230687977 2402 60-61 0.1508903618181492 2402 62-63 0.2998273067243815 2402 64-65 0.16387975541058353 2402 66-67 0.23615891616555018 2402 68-69 0.10616140556508924 2402 70-71 0.13530694842080493 2402 72-73 0.17112212798796378 2402 74-75 0.2025600513431911 2402 76-77 0.2506558326583388 2402 78-79 0.08448041755331559 2402 80-81 0.07413769480300658 2402 82-83 0.31033677404038684 2402 84-85 -0.1343402066246071 2402 86-87 0.2948033563859198 2402 88-89 0.46056039940202 2402 90-91 0.3990442815828956 2403 1 -0.012537947072722488 2403 2 0.0035752155724182444 2403 3 0.14754921671975296 2403 4 0.1089418491894989 2403 5 0.08433393238653508 2403 6 0.07399434366188729 2403 7 0.02128862665848885 2403 8 0.03912770470203952 2403 9 0.07273832161084215 2403 10-11 0.08329277034061988 2403 12-13 0.09489214751101116 2403 14-15 0.043043376232930086 2403 16-17 0.05953289506921777 2403 18-19 -0.001597585799402168 2403 20-21 0.014880206420031072 2403 22-23 0.02546004067286134 2403 24-25 -6.690193520455523E-4 2403 26-27 0.007473639637538554 2403 28-29 0.03990308235874096 2403 30-31 0.04240250987596994 2403 32-33 0.049995800893690046 2403 34-35 0.06548971494446221 2403 36-37 0.061307449208158005 2403 38-39 0.12464541437454102 2403 40-41 0.04192651800413927 2403 42-43 0.16884560578839114 2403 44-45 0.030420729163289195 2403 46-47 0.0862533005199353 2403 48-49 0.022470475427482484 2403 50-51 0.0471468090372511 2403 52-53 0.050129581202824625 2403 54-55 -0.005259248957493412 2403 56-57 0.06113522178397801 2403 58-59 0.0012148236492564024 2403 60-61 2.6844772803258365E-4 2403 62-63 0.08793389092242165 2403 64-65 -0.06236092306104268 2403 66-67 0.09245414765161541 2403 68-69 -0.017274948593872352 2403 70-71 -0.10564857008568396 2403 72-73 -0.20790554507357228 2403 74-75 0.2030010646663385 2403 76-77 -0.04059643045030725 2403 78-79 0.059529312988523486 2403 80-81 -0.11431568262356961 2403 82-83 -0.17459461923574082 2403 84-85 -0.49934579717928074 2403 86-87 -0.41795739765579754 2403 88-89 -0.3488919636887502 2403 90-91 0.3990442815828956 2404 1 -0.062092285414642845 2404 2 -0.1163044558613393 2404 3 0.06437532845514227 2404 4 0.07596013383474087 2404 5 0.1324979347039772 2404 6 -0.013577280387735868 2404 7 0.0646483469494612 2404 8 0.008464736259703898 2404 9 0.1002551172967614 2404 10-11 0.07395979773021111 2404 12-13 0.04869051262829771 2404 14-15 0.08720022685471918 2404 16-17 -0.04260109807650991 2404 18-19 0.0770257860814425 2404 20-21 0.02100511221482293 2404 22-23 0.031432817385322664 2404 24-25 0.03457671553648112 2404 26-27 0.11000147445466979 2404 28-29 -0.026703395050006407 2404 30-31 0.08126523476541081 2404 32-33 0.035534401508094504 2404 34-35 0.0718907629852481 2404 36-37 0.055651906180059996 2404 38-39 0.1670530219959474 2404 40-41 0.043072993166539675 2404 42-43 0.05733896427935292 2404 44-45 0.10703252291222753 2404 46-47 0.10045503117840582 2404 48-49 0.07200334340858205 2404 50-51 0.11671012637490463 2404 52-53 0.02034438579833875 2404 54-55 -0.012791095250051399 2404 56-57 -0.12836638017714108 2404 58-59 0.06913208467878462 2404 60-61 0.03034819034932923 2404 62-63 0.22702394906494305 2404 64-65 0.2256749574379171 2404 66-67 0.1769183225965918 2404 68-69 0.23669201084400981 2404 70-71 0.10680101914452678 2404 72-73 0.35305858725093486 2404 74-75 -0.10005692923778753 2404 76-77 0.2920395115485661 2404 78-79 0.24786666093956455 2404 80-81 0.3712462068237983 2404 82-83 0.05817330967932577 2404 84-85 0.18098818591308685 2404 86-87 0.4839530455972323 2404 88-89 0.20249588327298795 2404 90-91 0.3990442815828956 >>END_MODULE >>Per sequence quality scores pass #Quality Count 29 1.0 30 18.0 31 388.0 32 2596.0 33 4970.0 34 6455.0 35 8124.0 36 11588.0 37 17310.0 38 43310.0 39 260894.0 40 306688.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 30.28510950536128 14.379882296457117 24.634856312901793 30.70015188527981 2 32.14836312242645 16.002609219544382 27.23554095251613 24.613486705513033 3 29.36810494896134 18.567928000833408 21.543283595610117 30.520683454595144 4 26.484504983830103 20.72494270331641 20.757554254448607 32.03299805840487 5 25.707715953389638 21.57480576499754 22.177968481539747 30.539509800073073 6 25.5855736160473 23.979756681593496 24.5312844421764 25.903385260182805 7 25.156943089823685 23.703162414583403 23.706785920264757 27.433108575328152 8 24.49308665311878 24.569633210637402 24.206980683695132 26.730299452548685 9 24.276733168061213 24.66535415238653 24.81557865875937 26.242334020792885 10-11 24.832110903430554 23.75925126294271 24.105371545213803 27.303266288412935 12-13 24.781381823891586 23.195947108895403 24.18984452141039 27.832826545802618 14-15 24.95402677166781 23.16091988730897 24.132925286332437 27.75212805469078 16-17 25.417684519477852 23.11200256061068 23.57928381410208 27.891029105809384 18-19 25.35668884050838 23.24433600768183 23.593400388319026 27.805574763490764 20-21 25.318717520555843 23.383161569098743 23.60751696253597 27.69060394780944 22-23 25.413608075586332 22.8795697690921 23.586983763674958 28.119838391646613 24-25 25.38786608730837 23.134800450522537 23.623445289593594 27.8538881725755 26-27 25.524577937077826 23.371007727125864 23.539425251607177 27.564989084189133 28-29 25.7053002829354 23.12476032019712 23.392597781810608 27.777341615056873 30-31 25.719915148678542 23.25069639985808 23.549563287460273 27.479825164003106 32-33 25.639170639197722 23.27460873696338 23.690573553272667 27.39564707056623 34-35 25.788992426734264 22.954627155188014 23.627511453991428 27.6288689640863 36-37 25.693615080136585 23.316744644445738 23.66467787560419 27.32496239981349 38-39 25.885268272933338 23.393474367773333 23.569893198685605 27.151364160607727 40-41 25.806242037856723 23.199037339803795 23.622084313742107 27.37263630859737 42-43 25.630083836769852 23.322962742343808 23.780382276351432 27.26657114453491 44-45 25.810001169676777 23.38790380924738 23.890756520948045 26.911338500127798 46-47 25.824621162803517 23.253607149434107 23.703437832461237 27.21833385530114 48-49 25.782308577272143 23.454557075290808 23.825642336699477 26.937492010737568 50-51 25.908569859051507 23.47469289259719 23.776965613500114 26.83977163485119 52-53 25.913406625337522 23.2763164199128 23.693471880259846 27.116805074489836 54-55 25.73562472756229 23.414026380551295 23.96353539201856 26.886813499867856 56-57 25.913424301245946 23.558075713097278 23.922011111217355 26.60648887443942 58-59 25.817840600885216 23.369781780632334 23.961926226171755 26.850451392310692 60-61 26.106751983322717 23.47366186036636 23.695157025112437 26.724429131198484 62-63 26.07417445806307 23.37555532483429 24.088939404702707 26.46133081239993 64-65 26.09210591931382 23.38085323891687 23.733356604996757 26.793684236772553 66-67 25.935603023708627 23.37790631084641 23.847497422975607 26.83899324246936 68-69 26.057286374038547 23.581265852066654 23.70229922092514 26.659148552969654 70-71 26.191847904364106 23.20322627106438 23.674684334341542 26.930241490229967 72-73 26.161348517307477 23.43612583154809 23.750140940354044 26.652384710790393 74-75 26.168574179056588 23.75254037865012 23.60279174243235 26.476093699860947 76-77 26.168884323387314 23.296630980851642 23.385711763478483 27.148772932282554 78-79 26.21576682316119 23.378325508607197 23.597417840375588 26.808489827856025 80-81 26.627168440429607 23.42951882870692 23.00771678308891 26.935595947774566 82-83 26.749628238240586 23.163496908507476 22.960006261250683 27.12686859200125 84-85 26.847295797650705 22.68682342595113 23.128624971209614 27.337255805188548 86-87 27.21936241307685 21.97537553809567 22.670760709232667 28.13450133959481 88-89 26.26849369476715 21.63141693972793 22.83288650580876 29.267202859696155 90-91 22.581035316884375 22.08514755684567 25.374939525882922 29.958877600387034 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.5 7 2.5 8 3.5 9 3.0 10 9.5 11 15.0 12 14.0 13 17.0 14 30.0 15 56.0 16 95.5 17 130.5 18 167.5 19 234.5 20 355.0 21 516.5 22 691.0 23 942.5 24 1258.0 25 1620.5 26 2095.5 27 2703.5 28 3452.5 29 4266.0 30 5168.5 31 6184.0 32 7308.5 33 8487.0 34 9997.0 35 11739.0 36 13632.5 37 15619.5 38 17628.0 39 19988.0 40 22426.5 41 24888.5 42 27419.5 43 29770.5 44 31930.0 45 34057.5 46 36297.0 47 38501.0 48 40312.0 49 41912.0 50 43300.5 51 44517.5 52 45435.0 53 46006.0 54 46047.0 55 45424.5 56 44888.0 57 44309.5 58 43293.0 59 42014.5 60 40639.0 61 39152.0 62 37537.0 63 35574.5 64 33273.0 65 30779.5 66 27859.5 67 25254.5 68 22834.5 69 20221.5 70 17898.0 71 15587.5 72 13299.5 73 11131.5 74 9146.0 75 7602.0 76 6338.0 77 5156.0 78 4186.0 79 3355.5 80 2658.0 81 2103.0 82 1641.5 83 1257.5 84 954.0 85 742.5 86 563.5 87 419.5 88 309.0 89 220.5 90 163.5 91 119.5 92 77.5 93 52.5 94 44.5 95 33.5 96 19.0 97 12.5 98 8.5 99 3.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011323455254234217 3 1.5097940338978957E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 7.737434792768285E-5 34-35 0.0 36-37 0.0 38-39 0.06361944039736862 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 5.464421153867171E-4 64-65 0.0 66-67 3.579187739134481E-4 68-69 4.130814637954751E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0016050333846944016 78-79 9.780812003012491E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 10445.0 32-33 23600.0 34-35 26935.0 36-37 29874.0 38-39 32045.0 40-41 33855.0 42-43 34988.0 44-45 35632.0 46-47 35106.0 48-49 34674.0 50-51 33432.0 52-53 32742.0 54-55 30255.0 56-57 28612.0 58-59 26706.0 60-61 24552.0 62-63 23047.0 64-65 21185.0 66-67 19146.0 68-69 17355.0 70-71 15888.0 72-73 14150.0 74-75 12875.0 76-77 11483.0 78-79 10345.0 80-81 9272.0 82-83 8347.0 84-85 7483.0 86-87 6664.0 88-89 6077.0 90-91 5572.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.89965358480862 #Duplication Level Percentage of deduplicated Percentage of total 1 99.90689991658795 99.8066469239928 2 0.0876900346873368 0.17520408176209598 3 0.004284224378854353 0.01283977593981425 4 3.145704624095768E-4 0.0012570192089091912 5 8.112538834651694E-4 0.004052199096375056 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.529382101693687E-4 0.0 0.0 0.0 0.0 2 4.529382101693687E-4 0.0 0.0 0.0 0.0 3 4.529382101693687E-4 0.0 0.0 0.0 3.0195880677957914E-4 4 7.548970169489478E-4 0.0 0.0 0.0 3.0195880677957914E-4 5 9.058764203387374E-4 0.0 0.0 0.0 3.0195880677957914E-4 6 9.058764203387374E-4 0.0 0.0 0.0 3.0195880677957914E-4 7 0.0015097940338978956 0.0 0.0 0.0 3.0195880677957914E-4 8 0.005888196732201793 0.0 0.0 0.0 3.0195880677957914E-4 9 0.006341134942371162 0.0 0.0 0.0 3.0195880677957914E-4 10-11 0.006341134942371162 0.0 0.0 0.0 3.0195880677957914E-4 12-13 0.006341134942371162 0.0 0.0 0.0 3.0195880677957914E-4 14-15 0.006341134942371162 0.0 0.0 0.0 3.0195880677957914E-4 16-17 0.006341134942371162 0.0 0.0 0.0 3.0195880677957914E-4 18-19 0.006341134942371162 0.0 0.0 0.0 3.0195880677957914E-4 20-21 0.006416624644066057 0.0 0.0 0.0 4.529382101693687E-4 22-23 0.006718583450845635 0.0 0.0 0.0 4.529382101693687E-4 24-25 0.0070960319593201095 0.0 0.0 0.0 4.529382101693687E-4 26-27 0.007473480467794584 0.0 0.0 0.0 4.529382101693687E-4 28-29 0.007699949572879268 0.0 0.0 0.0 4.529382101693687E-4 30-31 0.008001908379658846 0.0 0.0 0.0 4.529382101693687E-4 32-33 0.008001908379658846 0.0 0.0 0.0 4.529382101693687E-4 34-35 0.008303867186438427 0.0 0.0 0.0 4.529382101693687E-4 36-37 0.008454846589828216 0.0 0.0 0.0 4.529382101693687E-4 38-39 0.008983274501692479 7.548970169489478E-5 0.0 0.0 4.529382101693687E-4 40-41 0.009511702413556743 1.5097940338978957E-4 0.0 0.0 4.529382101693687E-4 42-43 0.009738171518641427 1.5097940338978957E-4 0.0 0.0 4.529382101693687E-4 44-45 0.0101156200271159 1.5097940338978957E-4 0.0 0.0 4.529382101693687E-4 46-47 0.011096986149149533 1.5097940338978957E-4 0.0 0.0 4.529382101693687E-4 48-49 0.0115499243593189 1.5097940338978957E-4 0.0 0.0 4.529382101693687E-4 50-51 0.01245580077965764 1.5097940338978957E-4 0.0 0.0 4.529382101693687E-4 52-53 0.012833249288132113 1.5097940338978957E-4 0.0 0.0 4.529382101693687E-4 54-55 0.012833249288132113 1.5097940338978957E-4 0.0 0.0 4.529382101693687E-4 56-57 0.012833249288132113 3.0195880677957914E-4 0.0 0.0 4.529382101693687E-4 58-59 0.012833249288132113 3.0195880677957914E-4 0.0 0.0 4.529382101693687E-4 60-61 0.013286187498301481 3.0195880677957914E-4 0.0 0.0 4.529382101693687E-4 62-63 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