##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-30A_S29_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1172342 Sequences flagged as poor quality 0 Sequence length 61 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.44226172908588 40.0 40.0 40.0 40.0 40.0 2 39.181662006479336 40.0 40.0 40.0 40.0 40.0 3 39.144565323088315 40.0 40.0 40.0 40.0 40.0 4 39.045621499528295 40.0 40.0 40.0 40.0 40.0 5 39.05908344152133 40.0 40.0 40.0 40.0 40.0 6 39.15183112095276 40.0 40.0 40.0 40.0 40.0 7 39.17997649150163 40.0 40.0 40.0 40.0 40.0 8 39.19514271432739 40.0 40.0 40.0 40.0 40.0 9 39.21042835623052 40.0 40.0 40.0 40.0 40.0 10 39.21822130402221 40.0 40.0 40.0 40.0 40.0 11 39.206057617998844 40.0 40.0 40.0 40.0 40.0 12 39.21831342731046 40.0 40.0 40.0 40.0 40.0 13 39.04631583616385 40.0 40.0 40.0 40.0 40.0 14 39.21276214619966 40.0 40.0 40.0 40.0 40.0 15 39.12390070474316 40.0 40.0 40.0 40.0 40.0 16 39.30243222540863 40.0 40.0 40.0 40.0 40.0 17 39.24541473392576 40.0 40.0 40.0 40.0 40.0 18 39.33973192123118 40.0 40.0 40.0 40.0 40.0 19 39.27820380059744 40.0 40.0 40.0 40.0 40.0 20 39.299996076230315 40.0 40.0 40.0 40.0 40.0 21 39.269520327685946 40.0 40.0 40.0 40.0 40.0 22 39.333088808555864 40.0 40.0 40.0 40.0 40.0 23 39.326711829824404 40.0 40.0 40.0 40.0 40.0 24 39.32939705307837 40.0 40.0 40.0 40.0 40.0 25 39.2734782171073 40.0 40.0 40.0 40.0 40.0 26 39.241610383318175 40.0 40.0 40.0 40.0 40.0 27 39.25877261072281 40.0 40.0 40.0 40.0 40.0 28 39.266974995351184 40.0 40.0 40.0 40.0 40.0 29 39.27426297104429 40.0 40.0 40.0 40.0 40.0 30 39.26800541139019 40.0 40.0 40.0 40.0 40.0 31 39.21039764846777 40.0 40.0 40.0 40.0 40.0 32 39.238539607042995 40.0 40.0 40.0 40.0 40.0 33 39.24176392213194 40.0 40.0 40.0 40.0 40.0 34 39.23008132439169 40.0 40.0 40.0 40.0 40.0 35 39.23967579426481 40.0 40.0 40.0 40.0 40.0 36 39.20413326486639 40.0 40.0 40.0 40.0 40.0 37 39.21835095902049 40.0 40.0 40.0 40.0 40.0 38 39.2040547894727 40.0 40.0 40.0 40.0 40.0 39 37.97771469417627 40.0 40.0 40.0 40.0 40.0 40 39.199673815320104 40.0 40.0 40.0 40.0 40.0 41 39.20227032725945 40.0 40.0 40.0 40.0 40.0 42 39.1660965827378 40.0 40.0 40.0 40.0 40.0 43 39.191672737136436 40.0 40.0 40.0 40.0 40.0 44 39.15816715600055 40.0 40.0 40.0 40.0 40.0 45 39.03325138909977 40.0 40.0 40.0 40.0 40.0 46 39.04888164034045 40.0 40.0 40.0 40.0 40.0 47 39.05049550387174 40.0 40.0 40.0 40.0 40.0 48 39.20509202945898 40.0 40.0 40.0 40.0 40.0 49 39.189748384003984 40.0 40.0 40.0 40.0 40.0 50 39.169433492956834 40.0 40.0 40.0 40.0 40.0 51 39.12754469258971 40.0 40.0 40.0 40.0 40.0 52 39.14649479418122 40.0 40.0 40.0 40.0 40.0 53 39.04863597823843 40.0 40.0 40.0 40.0 40.0 54 38.93527315407961 40.0 40.0 40.0 40.0 40.0 55 39.016655549319225 40.0 40.0 40.0 40.0 40.0 56 39.021425488466676 40.0 40.0 40.0 40.0 40.0 57 38.915057210267996 40.0 40.0 40.0 40.0 40.0 58 38.90888153798124 40.0 40.0 40.0 40.0 40.0 59 38.904306081331214 40.0 40.0 40.0 40.0 40.0 60 38.854910938958085 40.0 40.0 40.0 40.0 40.0 61 38.77659590802001 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.05107459725369523 1101 2 -0.03774754984425499 1101 3 -0.01475260217918617 1101 4 -0.08471808362195077 1101 5 0.029573843696731217 1101 6 0.0771054046410029 1101 7 0.028826477487847058 1101 8 0.06526741362618083 1101 9 -0.03435048848148625 1101 10 0.04256742371847366 1101 11 0.023422457234168803 1101 12 -0.08116564534946491 1101 13 0.06691390515960904 1101 14 0.01322717773355464 1101 15 0.05377429629123043 1101 16 0.04968060223121995 1101 17 0.11225699084640439 1101 18 0.03654895896275434 1101 19 -0.0023218960640889463 1101 20 -0.039739945784305064 1101 21 0.01942630854694727 1101 22 0.04545075940208676 1101 23 0.006491380481385534 1101 24 0.04312777237269927 1101 25 0.13522373089990936 1101 26 0.0738598084094022 1101 27 0.15757927095459223 1101 28 0.04146274378155823 1101 29 0.10345417963688419 1101 30 0.05409705427042866 1101 31 -0.011787443206607406 1101 32 0.15734379857663328 1101 33 0.10838967284673373 1101 34 0.14526302049905837 1101 35 0.14192671945675528 1101 36 -0.037675293524756626 1101 37 -0.010896991588317917 1101 38 -0.0010556708140754267 1101 39 1.2468063893577437 1101 40 -0.1249804527488152 1101 41 0.010242237429800127 1101 42 0.00418489734197891 1101 43 0.053870290651616415 1101 44 0.025749058210713827 1101 45 -0.041947015695605216 1101 46 0.02196647998078305 1101 47 0.0534810033627906 1101 48 -0.019220817862951378 1101 49 -0.006147120021381625 1101 50 -0.007574147750162297 1101 51 -0.029189290218631925 1101 52 -0.021220319503363783 1101 53 0.024316203706327144 1101 54 0.10398185875850885 1101 55 0.03632886119747525 1101 56 -0.015454430029663513 1101 57 0.11499963825358606 1101 58 0.13574497928593132 1101 59 0.05214063091413834 1101 60 0.02771248421245076 1101 61 0.12931821986767744 1102 1 0.07186085456994107 1102 2 0.06949435845650243 1102 3 0.11860678188561735 1102 4 -0.07770408116349614 1102 5 -0.06135700516939835 1102 6 -0.02158842899957847 1102 7 -0.05169939715380423 1102 8 -0.006410536537096334 1102 9 0.005996081392154906 1102 10 0.0030771598463559258 1102 11 -0.09243974086623297 1102 12 -0.004579063750135504 1102 13 -0.10945116434889002 1102 14 -0.06939379225848796 1102 15 -0.06772724769984961 1102 16 0.03635648084756582 1102 17 -0.017779355061833257 1102 18 -0.0537630660996129 1102 19 -0.03751407467924395 1102 20 -0.031041589420858884 1102 21 -0.015598034091915736 1102 22 -0.040473268273146346 1102 23 0.0274140758872079 1102 24 -0.03861205874378015 1102 25 0.02053039049158656 1102 26 0.014268332022254526 1102 27 0.04064098164561614 1102 28 -0.01171157543019774 1102 29 -0.0404820107233661 1102 30 -0.04935498638526781 1102 31 -0.06238256874178205 1102 32 -0.04113223363246021 1102 33 0.1550349442322272 1102 34 -0.0286398838197357 1102 35 0.04522151378704109 1102 36 -0.13019173567037257 1102 37 -0.02299516821313574 1102 38 -0.002294745598277359 1102 39 1.2927503461279954 1102 40 -0.06274927039431333 1102 41 -0.05102017740164655 1102 42 -0.03819168862593614 1102 43 0.03346965647138944 1102 44 0.007245712694526674 1102 45 -0.060559682944656856 1102 46 0.0821025669848936 1102 47 -0.08879909119052343 1102 48 -0.062381209257594605 1102 49 -0.06009468355250647 1102 50 0.008025464114872705 1102 51 0.009678153755054097 1102 52 0.0691342354237463 1102 53 -0.06560905647868509 1102 54 -0.04925923804960064 1102 55 0.018081836611081314 1102 56 0.01852750277000581 1102 57 -0.07476319495470563 1102 58 0.13139779915155003 1102 59 -0.11547984170683634 1102 60 -0.07706568900426447 1102 61 -0.02360661459052693 1103 1 0.08943368089781245 1103 2 0.08532117684727325 1103 3 0.05663791460291634 1103 4 -0.15823966060650463 1103 5 -0.15263180361280604 1103 6 -0.019857834570508714 1103 7 -0.0532193403065051 1103 8 0.006663258058786425 1103 9 0.0807267624508512 1103 10 0.05428825483195254 1103 11 0.006373975614565097 1103 12 -0.046003152009021164 1103 13 -0.023656153444548522 1103 14 -0.020869785505659877 1103 15 -0.0016332689724833926 1103 16 0.00279727777730443 1103 17 0.045128594469204586 1103 18 -0.0572176899450767 1103 19 8.746942395845281E-4 1103 20 -0.08449221003576923 1103 21 -0.03811231691922501 1103 22 -0.06429884102412586 1103 23 0.03632622331569735 1103 24 0.0026376285261306975 1103 25 -0.04591638630840578 1103 26 0.047221555878763866 1103 27 0.044633080224706134 1103 28 -0.011813761279711343 1103 29 0.039522373563364965 1103 30 0.008279259917742365 1103 31 0.011654219020350354 1103 32 -0.03445161964393151 1103 33 0.017819614242576165 1103 34 0.09837969604514285 1103 35 0.11422293682490192 1103 36 -0.03128211291740968 1103 37 0.05196928438397208 1103 38 9.85928714669626E-6 1103 39 1.137056788931531 1103 40 -0.008837671715248518 1103 41 0.08163275421190264 1103 42 0.09475495594612937 1103 43 0.022969917716082477 1103 44 0.03001117861561653 1103 45 0.1935351366751945 1103 46 0.016638829474658223 1103 47 0.01405638961745126 1103 48 -0.0011068061421255493 1103 49 -0.007212650122603748 1103 50 0.10004439247359898 1103 51 0.028349335247540353 1103 52 0.11516753345348008 1103 53 0.04882339603451413 1103 54 0.09971973835360615 1103 55 0.02887015048889907 1103 56 0.05700161685366112 1103 57 0.0500023020788376 1103 58 0.02812643906217005 1103 59 0.06173040182516587 1103 60 0.09164429028596999 1103 61 0.0898936061223452 1104 1 0.14264788100459214 1104 2 0.05091672711972706 1104 3 0.006927791288141805 1104 4 0.0012771760094310025 1104 5 0.008037097763356371 1104 6 -0.036301727372524795 1104 7 -0.02408023613060095 1104 8 -0.08862986223390834 1104 9 -0.004484493169933046 1104 10 0.003572021881808496 1104 11 -0.09655092470472226 1104 12 -0.028031057128963255 1104 13 -0.016596655962850093 1104 14 -0.08068888768549698 1104 15 0.04451970295941976 1104 16 0.0076518721475338225 1104 17 0.07480190594003489 1104 18 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0.05752074635956461 2304 19 -0.08041507607282483 2304 20 0.07052446132002643 2304 21 -0.022209579464643525 2304 22 0.026960220471330842 2304 23 -0.020578471601488957 2304 24 -0.017922912140598157 2304 25 -0.02889005334820638 2304 26 3.945009919945619E-4 2304 27 -0.09628567011201739 2304 28 0.009454339263228917 2304 29 -0.019858780920543495 2304 30 -0.04676135593471287 2304 31 0.010723376712817867 2304 32 0.02608725125332967 2304 33 0.09823181875252374 2304 34 0.002073733266861666 2304 35 0.03178622512262308 2304 36 -0.025430676546918107 2304 37 -0.020601696083303978 2304 38 0.06703768877838456 2304 39 1.211877354859709 2304 40 0.025045586891302207 2304 41 0.06467825474167199 2304 42 0.14686536402021488 2304 43 -0.07681697626367168 2304 44 5.46278254653032E-4 2304 45 0.031523679233544044 2304 46 -0.052262058267558587 2304 47 -0.0332435690782944 2304 48 0.029617574742985653 2304 49 -0.050140343728827474 2304 50 -0.007610663107563198 2304 51 0.05545330823814254 2304 52 -0.028189963867724543 2304 53 -0.007680228563671676 2304 54 -0.0368087885210997 2304 55 -0.06193935308181864 2304 56 -0.031000394484060223 2304 57 0.022776212155278586 2304 58 -0.029105696846791318 2304 59 -0.09637570821176666 2304 60 0.07329083359857691 2304 61 -1.1783392301631466 2401 1 -0.041883657742630476 2401 2 -0.11111208589102262 2401 3 0.07249640750892183 2401 4 0.14866412319949518 2401 5 0.04973437769462663 2401 6 0.05700064219110601 2401 7 0.0161440274730964 2401 8 0.09723449080079405 2401 9 0.036541606782186875 2401 10 0.0053678012037963185 2401 11 9.682449412409255E-4 2401 12 0.0017848456603104523 2401 13 0.12959290614441699 2401 14 0.03830147864453437 2401 15 0.06266721638674966 2401 16 0.02733206234502461 2401 17 0.014384968936987264 2401 18 0.005265817087270364 2401 19 -0.03504100853328396 2401 20 -0.028636711181292185 2401 21 0.04392469484123751 2401 22 0.030213385420104544 2401 23 -0.04806787018383574 2401 24 0.03278126961567551 2401 25 0.011878201209633232 2401 26 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2401 61 0.02930926706655157 2402 1 -0.008855183099448993 2402 2 -0.11458984650695214 2402 3 -0.11149717248072477 2402 4 -0.04332692917882497 2402 5 0.13241480852077814 2402 6 -0.1438502080854107 2402 7 -0.03253574696972095 2402 8 0.030425378241766055 2402 9 -0.03953551596379157 2402 10 -0.009730545368135779 2402 11 -0.05442071573160945 2402 12 0.0010648130400525702 2402 13 0.07528812548584796 2402 14 -0.02444601344328845 2402 15 0.01934622275397402 2402 16 -0.006997550717770196 2402 17 0.02177777304788009 2402 18 -0.020017640293332306 2402 19 -0.025648453817460393 2402 20 0.05769406776517627 2402 21 -0.03373509204073599 2402 22 0.0029834225157046035 2402 23 -0.04383886431386941 2402 24 -0.08119778272898515 2402 25 -0.07708484318498421 2402 26 -0.027588362694437762 2402 27 -0.08437980126784339 2402 28 -0.05007874923590805 2402 29 -0.03900639597129185 2402 30 -0.023480993738196787 2402 31 -0.1081278163078494 2402 32 -0.08094169513796601 2402 33 -0.016319070669581492 2402 34 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2403 8 0.02419526499493685 2403 9 -0.040183252896270005 2403 10 0.06730883748439709 2403 11 0.06040815153881596 2403 12 0.1535931413184315 2403 13 0.09131167585652378 2403 14 0.005984057211982474 2403 15 0.0464740990809247 2403 16 0.010049861791308956 2403 17 -0.032216233458626675 2403 18 0.05807396823771427 2403 19 0.04804310640307108 2403 20 0.06249120563734323 2403 21 0.04106665413594612 2403 22 -0.01219847063190116 2403 23 -0.03567232285527666 2403 24 0.09018705863905296 2403 25 0.0687885353434865 2403 26 -0.009386985721640428 2403 27 0.08816455395219691 2403 28 0.00808500596731676 2403 29 0.014713532413850317 2403 30 -0.05747870446138137 2403 31 0.04446710462993764 2403 32 0.007236543197933543 2403 33 0.03185486072440824 2403 34 0.006831940378908996 2403 35 0.07268441545714666 2403 36 -0.039022658260030596 2403 37 -0.018503569366174588 2403 38 0.1134197611462966 2403 39 1.2416496731837086 2403 40 0.03444568106999668 2403 41 -0.03195251799141374 2403 42 -0.06431570543813336 2403 43 -0.010566629330561739 2403 44 0.027202591501733764 2403 45 0.00732879793924468 2403 46 0.07961275267338408 2403 47 0.024181735053403486 2403 48 -0.014526038413343656 2403 49 0.024656252116706412 2403 50 0.03024137654728065 2403 51 -0.07802534718944543 2403 52 -0.0019331304548728667 2403 53 -0.01054020480069795 2403 54 -0.01263261822605699 2403 55 -0.15147148454530424 2403 56 0.020058803999098984 2403 57 -0.10331159856424676 2403 58 -0.01786288109088474 2403 59 -0.09224712622484788 2403 60 -0.008469996171875493 2403 61 -0.03066921734611583 2404 1 0.01379500262043365 2404 2 -0.08242856549451716 2404 3 -0.0545639940453313 2404 4 0.0786279135443877 2404 5 0.10944963266593533 2404 6 0.07427000112382132 2404 7 0.08800876456212592 2404 8 0.10381959552007913 2404 9 0.07631749187367376 2404 10 0.027809366519512935 2404 11 0.13292777674197964 2404 12 0.08215825020997869 2404 13 0.1622381357328777 2404 14 0.14248784408536608 2404 15 0.11401455239069236 2404 16 0.03906892281153773 2404 17 0.005941863808693881 2404 18 0.04192779213749276 2404 19 -0.03340315320348708 2404 20 0.08183401433508664 2404 21 0.08514776315838901 2404 22 0.09275924897827537 2404 23 0.12779256024190033 2404 24 0.06051533917189289 2404 25 0.01293321341986342 2404 26 0.14210491755643773 2404 27 0.03702721934384812 2404 28 0.11620572535812101 2404 29 0.11080703528082125 2404 30 0.118289426656915 2404 31 0.12240827931818643 2404 32 0.0865651473330118 2404 33 -0.058492581424111734 2404 34 -0.009890841582262055 2404 35 0.0055411929848503405 2404 36 0.14725466764620876 2404 37 0.12950870085502686 2404 38 0.052641928020818796 2404 39 1.4163815499433667 2404 40 0.07578647301072294 2404 41 0.1293635008041818 2404 42 0.044041655664393886 2404 43 0.1321349888018304 2404 44 0.024338045096321537 2404 45 0.17528502558554493 2404 46 0.08320479811828818 2404 47 0.12608129231221454 2404 48 0.08084420739103848 2404 49 0.07395317474426122 2404 50 -0.004338007202548511 2404 51 0.16594347064430792 2404 52 0.13375498485903137 2404 53 0.11918793295932062 2404 54 0.07398067225118865 2404 55 0.13179500735191851 2404 56 0.18688636278987047 2404 57 0.1435578224786198 2404 58 0.16230296808623734 2404 59 0.1238882631340914 2404 60 0.06396842547336234 2404 61 0.035975572839646475 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 6.0 17 43.0 18 119.0 19 198.0 20 295.0 21 348.0 22 548.0 23 704.0 24 1110.0 25 1322.0 26 1810.0 27 2221.0 28 3014.0 29 3642.0 30 4444.0 31 5726.0 32 7314.0 33 8813.0 34 11276.0 35 14497.0 36 21314.0 37 31160.0 38 75341.0 39 497579.0 40 479498.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.445161906679107 15.203157440405615 25.857386325833247 29.494294327082027 2 31.8437656262533 16.31171783402423 28.256617851602435 23.58789868812004 3 28.977916834777595 19.478632923355917 22.35586744812303 29.18758279374346 4 26.170798428102405 21.36042328981073 21.89252103270294 30.576257249383925 5 25.52855736636579 21.83262222116072 22.98083665005604 29.65798376241745 6 25.96247511391727 23.92765933490398 24.88377964791844 25.22608590326031 7 25.388495848481075 23.866499707423262 23.994789916253108 26.75021452784256 8 24.29265521494581 25.183777430135574 24.286684261077397 26.236883093841218 9 24.19242848929749 25.249287324006136 25.07450897434366 25.48377521235271 10 25.04252172147718 23.810202142378248 24.4245279960967 26.72274814004787 11 24.80086868848851 24.265018228469167 24.29035213273942 26.643760950302898 12 25.146331019446542 23.908893479888974 24.486114120282306 26.458661380382175 13 24.43442271965007 22.75197851821397 24.494644054380036 28.31895470775593 14 24.6608071706038 23.536391257841142 24.38742278277158 27.415378788783478 15 24.719749015219108 23.728485373722002 24.245484679385367 27.30628093167352 16 25.116135052740585 23.73735650518364 23.76541998836517 27.381088453710607 17 25.07809154666471 23.457489367437148 23.997007699118516 27.467411386779627 18 25.43805476558888 23.983189205880194 24.05799672791728 26.520759300613644 19 25.02085568886895 23.45143311422776 24.24881135368348 27.27889984321981 20 25.050881056892955 23.650436476727783 23.86812039490183 27.43056207147744 21 25.05941099099068 23.823935336275593 24.448326512229364 26.668327160504358 22 25.725087047977468 23.108529763499046 23.69496273271793 27.471420455805557 23 25.144795631308952 23.876394430976628 23.91904410146527 27.05976583624915 24 25.185312818273164 24.50044440956649 23.809605046991404 26.504637725168934 25 25.377492233495 23.44128249265146 23.566160727842217 27.615064546011318 26 25.424662769055445 23.9358480716378 24.037354287400774 26.60213487190598 27 25.242292778046 24.55401239570023 23.6522277628883 26.551467063365468 28 25.563615395507455 23.968005923186237 23.4539067951161 27.014471886190204 29 25.56668617178264 24.991342116890806 23.312736385798683 26.129235325527876 30 25.88707049649334 25.147013414174364 23.312736385798683 25.653179703533613 31 25.580078168316074 24.621825371777177 23.74000078475394 26.058095675152813 32 25.69318509445196 25.05855799758091 23.249700172816464 25.998556735150665 33 25.864295572452406 25.980899771568367 23.135399055906895 25.019405600072336 34 26.303757777167412 25.300296329910555 22.683483147409202 25.712462745512827 35 26.45542000542504 25.93859129844363 22.943560838048967 24.66242785808237 36 27.135426351696008 26.660564920475426 22.10685960240271 24.097149125425858 37 26.781689984663178 26.642822657552145 21.65084932553811 24.924638032246563 38 27.325985079439274 27.203921722500773 21.49850470255267 23.971588495507284 39 27.430084068962422 27.46915725991328 21.435693359933612 23.665065311190688 40 27.35524275339449 27.935704768744955 20.54101960008257 24.168032877777986 41 27.347224615342625 28.307439296723995 20.511847225468337 23.83348886246505 42 27.511255248041955 28.371499101797937 20.11767897081227 23.999566679347836 43 27.614552749965455 28.751081169146886 19.675828384549902 23.95853769633776 44 27.659164305296578 28.822903214249767 19.71370129194382 23.804231188509835 45 27.83735462859814 29.1165035458936 19.06141723149047 23.984724594017788 46 27.848102345561276 29.02565974775279 19.21060577885975 23.915632127826182 47 28.160980328265982 29.27319843526889 18.609330724310823 23.956490512154303 48 28.341047237069045 29.499156389517733 18.327245803698922 23.832550569714297 49 28.20038862379749 29.471092906336203 18.06341494205616 24.265103527810144 50 27.918474301867548 29.64698014743138 18.071518379449 24.363027171252075 51 27.77900987936967 29.759660576862384 17.932053956951126 24.529275586816816 52 27.61719702953575 29.692444696172277 17.73620667006727 24.954151604224705 53 27.485921343771697 29.703704209181282 17.757616804652567 25.052757642394457 54 27.15547169682567 29.873620496408044 17.7691322156845 25.20177559108178 55 26.92900194653096 29.586673513360434 17.691253917372237 25.79307062273637 56 26.781689984663178 29.584541029836004 17.714967134163924 25.918801851336898 57 26.397928249606345 29.678370304911024 17.83626279703363 26.087438648449 58 26.094262595727187 29.581726151583755 17.72733553860563 26.596675714083435 59 25.801941754198005 29.3918498185683 17.890854375259096 26.915354051974592 60 25.602767793016028 29.331969681202242 17.935977726636086 27.12928479914564 61 25.578116283473594 28.883465746343646 17.958667351336043 27.57975061884672 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 9.0 1 8.0 2 4.5 3 2.0 4 5.0 5 8.0 6 7.5 7 11.5 8 16.0 9 14.5 10 13.0 11 19.5 12 28.0 13 30.0 14 35.0 15 40.0 16 53.5 17 101.0 18 135.0 19 152.0 20 268.0 21 367.0 22 439.5 23 512.0 24 725.5 25 1318.5 26 1698.0 27 2199.5 28 2701.0 29 3596.0 30 5517.5 31 6544.0 32 8046.0 33 9548.0 34 11098.0 35 14964.0 36 17280.0 37 20157.5 38 23035.0 39 26676.0 40 34553.5 41 38790.0 42 43043.0 43 51065.5 44 54835.0 45 58986.5 46 63138.0 47 66202.0 48 71711.0 49 74156.0 50 76570.5 51 78985.0 52 80138.0 53 82456.5 54 83622.0 55 82701.0 56 81780.0 57 79056.5 58 72452.5 59 68572.0 60 64156.5 61 56305.0 62 52869.0 63 45241.0 64 37613.0 65 32991.0 66 24498.0 67 20627.0 68 17617.5 69 14608.0 70 12446.5 71 8661.0 72 7037.0 73 5813.5 74 4590.0 75 3756.5 76 2453.5 77 1984.0 78 1591.5 79 1199.0 80 990.5 81 601.0 82 420.0 83 335.0 84 195.0 85 140.0 86 109.5 87 79.0 88 67.0 89 45.5 90 36.0 91 27.5 92 19.0 93 14.0 94 11.5 95 14.0 96 12.5 97 11.0 98 9.5 99 4.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.03719051266609914 3 8.529934097729162E-5 4 8.529934097729162E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 3.071885166615203 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 61 1172342.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.54868188519565 #Duplication Level Percentage of deduplicated Percentage of total 1 82.02331010902394 63.60799582815449 2 13.288464645682804 20.610058351014498 3 2.813143087086701 6.544666150740714 4 0.8513888664191582 2.640963374501465 5 0.35948028880978194 1.3938611280454019 6 0.20948398657331443 0.9747124220889934 7 0.12905228026955548 0.7005483960428007 8 0.08395518774291505 0.5208491317509756 9 0.061028199460258 0.4259390783372834 >10 0.17792567699098105 2.0045953012016122 >50 0.0021663121470736177 0.11077001535002749 >100 4.810878347854687E-4 0.04635485136733022 >500 0.0 0.0 >1k 1.2027195869636718E-4 0.4186859714043166 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGTAACATCTGGGGGGGGGGGGGGG 4489 0.382908741647062 TruSeq Adapter, Index 11 (97% over 36bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.48339136531831156 0.0 0.0 0.0 0.0 2 0.4845002567510163 0.0 0.0 0.0 0.0 3 0.48603564488860757 0.0 0.0 0.0 1.705986819545832E-4 4 0.48816812841303986 0.0 0.0 0.0 1.705986819545832E-4 5 0.48936231918672196 0.0 0.0 0.0 1.705986819545832E-4 6 0.49234779612092716 0.0 8.52993409772916E-5 0.0 1.705986819545832E-4 7 0.49669806251076903 0.0 8.52993409772916E-5 0.0 1.705986819545832E-4 8 0.5359357593603232 0.0 8.52993409772916E-5 0.0 1.705986819545832E-4 9 0.539688930363324 0.0 8.52993409772916E-5 0.0 1.705986819545832E-4 10 0.5452333875268479 0.0 8.52993409772916E-5 0.0 1.705986819545832E-4 11 0.5508631440313492 0.0 8.52993409772916E-5 0.0 2.5589802293187483E-4 12 0.5634874464959884 0.0 8.52993409772916E-5 0.0 2.5589802293187483E-4 13 0.5788413278719009 0.0 8.52993409772916E-5 0.0 2.5589802293187483E-4 14 0.5976924822278823 0.0 8.52993409772916E-5 0.0 4.2649670488645807E-4 15 0.621576297701524 0.0 8.52993409772916E-5 0.0 5.117960458637497E-4 16 0.6516016657255306 0.0 8.52993409772916E-5 0.0 5.117960458637497E-4 17 0.6893039744374935 0.0 8.52993409772916E-5 0.0 5.117960458637497E-4 18 0.7322948422900485 0.0 8.52993409772916E-5 0.0 5.117960458637497E-4 19 0.7935397691117438 0.0 8.52993409772916E-5 0.0 5.117960458637497E-4 20 0.8754271364499437 0.0 8.52993409772916E-5 0.0 5.117960458637497E-4 21 0.992201934247856 0.0 8.52993409772916E-5 0.0 5.117960458637497E-4 22 1.15836505047162 0.0 8.52993409772916E-5 0.0 5.117960458637497E-4 23 1.386199590221966 0.0 8.52993409772916E-5 0.0 5.117960458637497E-4 24 1.6960920959924664 0.0 8.52993409772916E-5 0.0 5.117960458637497E-4 25 2.1136323700763087 0.0 8.52993409772916E-5 0.0 5.117960458637497E-4 26 2.61877506734383 0.0 8.52993409772916E-5 0.0 5.117960458637497E-4 27 3.2719121212069515 0.0 8.52993409772916E-5 0.0 5.117960458637497E-4 28 4.040373884071371 0.0 8.52993409772916E-5 0.0 5.117960458637497E-4 29 4.948385368774641 0.0 8.52993409772916E-5 0.0 5.117960458637497E-4 30 6.008656177122376 0.0 8.52993409772916E-5 0.0 5.117960458637497E-4 31 7.200714467280026 0.0 8.52993409772916E-5 0.0 5.117960458637497E-4 32 8.53377256807314 0.0 8.52993409772916E-5 0.0 5.117960458637497E-4 33 9.99162360471603 0.0 8.52993409772916E-5 0.0 5.117960458637497E-4 34 11.60258695841316 0.0 8.52993409772916E-5 0.0 5.117960458637497E-4 35 13.348664468218319 0.0 8.52993409772916E-5 0.0 5.117960458637497E-4 36 15.215867042211231 0.0 1.705986819545832E-4 0.0 5.117960458637497E-4 37 17.188499601652076 0.0 2.5589802293187483E-4 0.0 5.117960458637497E-4 38 19.255899728918696 0.0 2.5589802293187483E-4 0.0 5.117960458637497E-4 39 21.401604651202465 0.0 2.5589802293187483E-4 0.0 5.117960458637497E-4 40 23.66152539105483 0.0 2.5589802293187483E-4 0.0 5.117960458637497E-4 41 25.955139370593223 0.0 2.5589802293187483E-4 0.0 5.970953868410413E-4 42 28.303942023743925 0.0 2.5589802293187483E-4 0.0 5.970953868410413E-4 43 30.688058604059226 0.0 2.5589802293187483E-4 0.0 5.970953868410413E-4 44 33.06731312193882 0.0 2.5589802293187483E-4 0.0 5.970953868410413E-4 45 35.45885074491915 0.0 2.5589802293187483E-4 0.0 5.970953868410413E-4 46 37.82940473001906 0.0 2.5589802293187483E-4 0.0 5.970953868410413E-4 47 40.209085744603534 0.0 2.5589802293187483E-4 0.0 5.970953868410413E-4 48 42.579725029044425 0.0 2.5589802293187483E-4 0.0 5.970953868410413E-4 49 44.87965115981514 0.0 2.5589802293187483E-4 0.0 5.970953868410413E-4 50 47.1403395937363 0.0 2.5589802293187483E-4 0.0 5.970953868410413E-4 >>END_MODULE