##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-30A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 689610 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.555110859761314 40.0 40.0 40.0 40.0 40.0 2 39.34844912341758 40.0 40.0 40.0 40.0 40.0 3 39.31737068778005 40.0 40.0 40.0 40.0 40.0 4 39.23289395455402 40.0 40.0 40.0 40.0 40.0 5 39.247650121082934 40.0 40.0 40.0 40.0 40.0 6 39.33945273415409 40.0 40.0 40.0 40.0 40.0 7 39.368541639477385 40.0 40.0 40.0 40.0 40.0 8 39.38858775249779 40.0 40.0 40.0 40.0 40.0 9 39.39925465117965 40.0 40.0 40.0 40.0 40.0 10-11 39.401467496121 40.0 40.0 40.0 40.0 40.0 12-13 39.32805498760169 40.0 40.0 40.0 40.0 40.0 14-15 39.358448978408084 40.0 40.0 40.0 40.0 40.0 16-17 39.45934078682154 40.0 40.0 40.0 40.0 40.0 18-19 39.4968257420861 40.0 40.0 40.0 40.0 40.0 20-21 39.4771044503415 40.0 40.0 40.0 40.0 40.0 22-23 39.51320601499398 40.0 40.0 40.0 40.0 40.0 24-25 39.485364191354535 40.0 40.0 40.0 40.0 40.0 26-27 39.456518901988076 40.0 40.0 40.0 40.0 40.0 28-29 39.47742637142733 40.0 40.0 40.0 40.0 40.0 30-31 39.44871273855075 40.0 40.0 40.0 40.0 40.0 32-33 39.45595873793045 40.0 40.0 40.0 40.0 40.0 34-35 39.450412454164194 40.0 40.0 40.0 40.0 40.0 36-37 39.42736026323668 40.0 40.0 40.0 40.0 40.0 38-39 39.36835836031133 40.0 40.0 40.0 40.0 40.0 40-41 39.41241931101833 40.0 40.0 40.0 40.0 40.0 42-43 39.38880763515229 40.0 40.0 40.0 40.0 40.0 44-45 39.30178498932132 40.0 40.0 40.0 40.0 40.0 46-47 39.27617040136103 40.0 40.0 40.0 40.0 40.0 48-49 39.36360533994542 40.0 40.0 40.0 40.0 40.0 50-51 39.316077247650405 40.0 40.0 40.0 40.0 40.0 52-53 39.26641700140517 40.0 40.0 40.0 40.0 40.0 54-55 39.1718910956757 40.0 40.0 40.0 40.0 40.0 56-57 39.19513092139637 40.0 40.0 40.0 40.0 40.0 58-59 39.15205043303392 40.0 40.0 40.0 40.0 40.0 60-61 39.052319887772526 40.0 40.0 40.0 40.0 40.0 62-63 39.38642801096607 40.0 40.0 40.0 40.0 40.0 64-65 39.38859592911895 40.0 40.0 40.0 40.0 40.0 66-67 39.38522213450254 40.0 40.0 40.0 40.0 40.0 68-69 39.38369943271489 40.0 40.0 40.0 40.0 40.0 70-71 39.3701232557869 40.0 40.0 40.0 40.0 40.0 72-73 39.39099724253123 40.0 40.0 40.0 40.0 40.0 74-75 39.37038323510966 40.0 40.0 40.0 40.0 40.0 76-77 39.3735007893807 40.0 40.0 40.0 40.0 40.0 78-79 39.37274763372142 40.0 40.0 40.0 40.0 40.0 80-81 39.381495892071136 40.0 40.0 40.0 40.0 40.0 82-83 39.360410589085916 40.0 40.0 40.0 40.0 40.0 84-85 39.35914545104469 40.0 40.0 40.0 40.0 40.0 86-87 39.34759119823059 40.0 40.0 40.0 40.0 40.0 88-89 39.42277549244301 40.0 40.0 40.0 40.0 40.0 90-91 39.493881599043505 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1316440598384503 1101 2 -0.04541406437783024 1101 3 -0.007022969134524715 1101 4 -0.15828005651633958 1101 5 0.03072656230576598 1101 6 -0.009495488794087237 1101 7 -0.06652245596450967 1101 8 0.030188195097586856 1101 9 -0.12406131724655722 1101 10-11 -0.0809400902749502 1101 12-13 0.013754329205447391 1101 14-15 -0.010077601513863499 1101 16-17 0.08877431746734032 1101 18-19 0.061543047203215906 1101 20-21 0.023826113163430307 1101 22-23 0.04297559262951722 1101 24-25 -0.026736267199289898 1101 26-27 0.06842188839840446 1101 28-29 0.0057583733380610624 1101 30-31 0.08079721066584966 1101 32-33 0.061398432052236274 1101 34-35 -0.015901456003120984 1101 36-37 -0.1701189430804746 1101 38-39 0.1147256336818856 1101 40-41 -0.10781060843115142 1101 42-43 -0.14727579757641962 1101 44-45 0.002538016818085964 1101 46-47 0.15471813557009284 1101 48-49 -0.020348960744001943 1101 50-51 -0.05554986213004298 1101 52-53 0.13899552909710167 1101 54-55 0.06813335668935849 1101 56-57 0.24875245948227587 1101 58-59 -5.139738892054879E-4 1101 60-61 -0.04080238748381504 1101 62-63 0.09330378550719587 1101 64-65 0.01740717816309001 1101 66-67 0.17993382055716722 1101 68-69 0.1944215176324846 1101 70-71 0.13475111866280542 1101 72-73 0.1996976262076373 1101 74-75 -0.07740907726272894 1101 76-77 -0.19193344662665623 1101 78-79 -8.321445452494913E-4 1101 80-81 -0.19480846926033735 1101 82-83 -0.1860341126227425 1101 84-85 0.04023942008186765 1101 86-87 -1.13618688273543 1101 88-89 -0.3469692462608478 1101 90-91 -0.579228795585216 1102 1 0.015731317172743786 1102 2 0.13461031437692839 1102 3 0.131777426462115 1102 4 0.035238588096333956 1102 5 0.023631719573288024 1102 6 0.06169404709424953 1102 7 -0.11588496938733783 1102 8 0.06338750481270239 1102 9 0.029513675330420597 1102 10-11 0.011367653551658918 1102 12-13 -0.061990595794163994 1102 14-15 -0.0044204802161686985 1102 16-17 0.019586205905511633 1102 18-19 -0.013969699179597228 1102 20-21 -0.06126623364891515 1102 22-23 0.08372740485059893 1102 24-25 -0.0378819570488389 1102 26-27 0.003431968075858549 1102 28-29 0.05276896202247627 1102 30-31 -0.11947693870089893 1102 32-33 0.03880383627321038 1102 34-35 0.008248635522086545 1102 36-37 0.0026323824206642144 1102 38-39 0.06843463047552945 1102 40-41 -0.005524978070084785 1102 42-43 0.05948881835512054 1102 44-45 0.09957013347985111 1102 46-47 0.032551622669373614 1102 48-49 -0.12350531153148125 1102 50-51 -0.08479217942334571 1102 52-53 0.11729394347010214 1102 54-55 0.06041961250166139 1102 56-57 -0.15611945480475242 1102 58-59 -0.08886968965821751 1102 60-61 -0.10337523503563517 1102 62-63 0.03637812298760679 1102 64-65 -0.005577822423603607 1102 66-67 -0.14795882785423942 1102 68-69 -0.10915443826737459 1102 70-71 -0.12148660528484356 1102 72-73 -0.0072442233765528385 1102 74-75 -0.3244006848863634 1102 76-77 -0.5425066086149215 1102 78-79 -0.0449559996927249 1102 80-81 0.13339806085242856 1102 82-83 0.04248575720147585 1102 84-85 0.29892632233806893 1102 86-87 -0.18514229713935038 1102 88-89 -0.04956871575687671 1102 90-91 0.5636283472719228 1103 1 0.04727231216888583 1103 2 0.1219579611930186 1103 3 -0.034332211408319324 1103 4 0.09071688609954975 1103 5 0.022405924327557614 1103 6 0.05284353990487034 1103 7 -0.10218549057157844 1103 8 -0.19477847096124634 1103 9 -0.06325180946164011 1103 10-11 0.01927281594187491 1103 12-13 -0.09973081791977023 1103 14-15 -0.047219320357200445 1103 16-17 -0.04430615865129539 1103 18-19 -0.04656998925497646 1103 20-21 -0.042916366461838606 1103 22-23 -0.09222620964122541 1103 24-25 -0.01247934194523026 1103 26-27 -0.09998842105881067 1103 28-29 0.02929354485934965 1103 30-31 0.004297404342544553 1103 32-33 0.046503398592129486 1103 34-35 -0.004060752316199512 1103 36-37 -0.04281950400822154 1103 38-39 -0.011734903893177773 1103 40-41 0.042276031686029114 1103 42-43 0.0999031117804492 1103 44-45 0.054055237299401426 1103 46-47 0.005522311203833397 1103 48-49 0.03156285534321057 1103 50-51 0.09279919696729877 1103 52-53 -0.16765431174093237 1103 54-55 0.03062156137894334 1103 56-57 0.060947893329185376 1103 58-59 -0.06716980290048014 1103 60-61 0.30675150783374505 1103 62-63 -0.02663501929649925 1103 64-65 -0.007552507239324768 1103 66-67 -0.204912563410673 1103 68-69 -0.025778348672325535 1103 70-71 -0.26410978414703123 1103 72-73 0.0351467827171561 1103 74-75 -0.13480677668934504 1103 76-77 0.00962129492808117 1103 78-79 -0.32579589470584835 1103 80-81 0.1853260771435714 1103 82-83 0.19741194212765834 1103 84-85 0.09554581931205774 1103 86-87 0.41607119913937396 1103 88-89 0.283764617576459 1103 90-91 -0.594266389570187 1104 1 -0.05214496830024018 1104 2 -0.00330665359043536 1104 3 0.06952603403888702 1104 4 -0.1459779123259466 1104 5 0.05733029375398502 1104 6 -0.03782515979030876 1104 7 -0.010838021678416965 1104 8 -0.04058856041176995 1104 9 0.042559673597139636 1104 10-11 -0.09753833511022947 1104 12-13 -0.051128866594957856 1104 14-15 -0.04996500187945685 1104 16-17 -0.0765045210249724 1104 18-19 -0.12376440990333037 1104 20-21 -0.03651615222872806 1104 22-23 -0.03252075284513012 1104 24-25 -0.03643847369995967 1104 26-27 0.019382597081317954 1104 28-29 -0.015605780606925634 1104 30-31 -0.07500109151693835 1104 32-33 0.012865670804053764 1104 34-35 -0.015860495803032393 1104 36-37 -0.15186565760666326 1104 38-39 -0.10468919359178841 1104 40-41 -0.0663526140247157 1104 42-43 -0.09522535405882593 1104 44-45 -0.02167765578474956 1104 46-47 0.0012104807639019555 1104 48-49 -0.09685657831673211 1104 50-51 0.05907304091531529 1104 52-53 0.15844778394463788 1104 54-55 0.053082819114173674 1104 56-57 -0.06352621423930316 1104 58-59 0.1461496744104025 1104 60-61 -0.11081729464346779 1104 62-63 -0.25982916785866195 1104 64-65 -0.22133369038966322 1104 66-67 -0.28185914811516 1104 68-69 -0.035821476625990556 1104 70-71 -0.01400710252156756 1104 72-73 -0.3103690937404977 1104 74-75 -0.3454717628082875 1104 76-77 0.1882023966431845 1104 78-79 -0.2853689790437599 1104 80-81 0.3009376200418217 1104 82-83 0.05915615773915306 1104 84-85 -0.09480228021406134 1104 86-87 -0.08820674053036726 1104 88-89 0.12525645906830363 1104 90-91 0.5636283472719228 1201 1 0.06535717139185948 1201 2 0.016668861831597326 1201 3 0.06332146864014732 1201 4 0.0801360818020882 1201 5 0.08062206108548509 1201 6 0.043543809798975985 1201 7 0.08477208850217721 1201 8 0.05517647431082651 1201 9 -0.08235284278911337 1201 10-11 -0.022323011664624914 1201 12-13 0.05745752821344752 1201 14-15 0.0065429122728488665 1201 16-17 0.03657847736077713 1201 18-19 0.11070900916213589 1201 20-21 0.04297043000586598 1201 22-23 0.1183172480865835 1201 24-25 0.10940998192987195 1201 26-27 0.020878214775223114 1201 28-29 -0.022275119631665063 1201 30-31 0.09498556953739978 1201 32-33 -0.0057951576276806804 1201 34-35 0.11560254697305794 1201 36-37 0.1394693770074653 1201 38-39 0.09988298710722177 1201 40-41 0.10564269047520725 1201 42-43 0.06268768273725556 1201 44-45 0.05402067430586044 1201 46-47 0.0018068814497809171 1201 48-49 0.031085221157141518 1201 50-51 0.06995198234583455 1201 52-53 0.1394040763462101 1201 54-55 0.10585594444239632 1201 56-57 0.031098277295072307 1201 58-59 0.19328168781913746 1201 60-61 0.1954645751118349 1201 62-63 -0.09121751379537102 1201 64-65 -0.11054052982533591 1201 66-67 0.2159602939323335 1201 68-69 -0.01962049102480279 1201 70-71 -0.06906172536600508 1201 72-73 0.06283657698516976 1201 74-75 0.19898974939516023 1201 76-77 0.3483456934531475 1201 78-79 0.2614165493268814 1201 80-81 0.15555965980941977 1201 82-83 0.49863541278934065 1201 84-85 0.34249930708752174 1201 86-87 -0.025843886678146077 1201 88-89 0.2726766064884458 1201 90-91 0.19999198363556303 1202 1 0.030196655995489152 1202 2 -0.041974818396553815 1202 3 -0.024271506964595346 1202 4 -0.00916760210868972 1202 5 0.028309500332859727 1202 6 0.02807249788482835 1202 7 -0.0672111769594963 1202 8 0.02714997653110629 1202 9 -0.006623490117711128 1202 10-11 0.07374642724875002 1202 12-13 -0.046716899801914735 1202 14-15 0.08720205953079585 1202 16-17 0.011316090248257638 1202 18-19 -0.003914870527324865 1202 20-21 0.0041583338636357325 1202 22-23 0.05729270723726643 1202 24-25 -0.07940489759258895 1202 26-27 -0.04141276407537475 1202 28-29 0.01671361410162575 1202 30-31 -0.07698855133527616 1202 32-33 -0.03150516709979456 1202 34-35 -0.10166638729342736 1202 36-37 -0.21097621757912322 1202 38-39 -0.18600407507427974 1202 40-41 -0.14401581335069125 1202 42-43 -0.20872249733015025 1202 44-45 -0.053404248681836464 1202 46-47 -0.10731129917197535 1202 48-49 -0.15597073239909065 1202 50-51 -0.09617658561808184 1202 52-53 -0.05961280912889322 1202 54-55 -0.14551333342935635 1202 56-57 -0.2021667547950443 1202 58-59 -0.22069451394011708 1202 60-61 0.1224535811702907 1202 62-63 -0.2460598689857676 1202 64-65 -0.2684020691330673 1202 66-67 -0.4417691411375273 1202 68-69 -0.3159428055820612 1202 70-71 -0.23168211836039632 1202 72-73 -0.23114555900775002 1202 74-75 -0.2444605174053578 1202 76-77 -0.08496193192258517 1202 78-79 -0.6265886527539521 1202 80-81 -0.9159430998289366 1202 82-83 -0.9246883288750496 1202 84-85 -0.6592928076078195 1202 86-87 0.25221879138452863 1202 88-89 0.08889282270466481 1202 90-91 -2.063822633120232 1203 1 0.10966262764517865 1203 2 0.15632403786040072 1203 3 0.10366939573354728 1203 4 9.750150828651272E-4 1203 5 0.15022949515986994 1203 6 -0.11275500409546169 1203 7 0.07660018391899825 1203 8 -0.13229613749068392 1203 9 0.07858388077512046 1203 10-11 0.022917574657739692 1203 12-13 -0.020783609542114334 1203 14-15 0.03798246743608047 1203 16-17 -0.04239493433337316 1203 18-19 0.05193210058472886 1203 20-21 0.048220053123294804 1203 22-23 -0.009502032785810854 1203 24-25 -0.07715318442892993 1203 26-27 -0.0130134359390226 1203 28-29 -0.0635298712875354 1203 30-31 0.07020820135852546 1203 32-33 0.025829679263814853 1203 34-35 -0.006659321983590871 1203 36-37 0.03837275067002821 1203 38-39 -0.006951831471106118 1203 40-41 -0.0791883516094316 1203 42-43 -0.041871523377189135 1203 44-45 -0.09579519552018922 1203 46-47 -0.04140314047674565 1203 48-49 0.04374122002048608 1203 50-51 -0.007971783008038358 1203 52-53 0.01350511061303905 1203 54-55 0.06243402871002246 1203 56-57 -0.08526988883164677 1203 58-59 -0.1686432331513359 1203 60-61 0.16403243850288618 1203 62-63 0.019207748217866083 1203 64-65 0.06601069672588977 1203 66-67 0.19026606715688388 1203 68-69 0.12137419689522488 1203 70-71 0.06538598903910042 1203 72-73 0.009328753864203065 1203 74-75 -0.3302494347071274 1203 76-77 -0.2848593816280669 1203 78-79 -0.25652649795109994 1203 80-81 0.2480913331710184 1203 82-83 -0.00398588709624903 1203 84-85 -0.49887496191832525 1203 86-87 0.2611931503588849 1203 88-89 -0.6311071772953412 1203 90-91 -0.10303831939474861 1204 1 -0.07750954189075543 1204 2 -0.12950562975450453 1204 3 -0.1900983135489298 1204 4 -0.30058082680692877 1204 5 -0.35543314112862845 1204 6 -0.2125159144411768 1204 7 -0.2495040314319965 1204 8 -0.008043961897200802 1204 9 -0.09217987529326876 1204 10-11 -0.20628573007220297 1204 12-13 -0.12266670189068662 1204 14-15 -0.1740251463125162 1204 16-17 -0.049584960383903365 1204 18-19 -0.11591148817699093 1204 20-21 -0.15792581043192655 1204 22-23 -0.150014752365621 1204 24-25 -0.07322648486044869 1204 26-27 -0.1413253936340766 1204 28-29 -0.03638970274691644 1204 30-31 -0.14098765992767426 1204 32-33 -0.19405246259583464 1204 34-35 -0.0903751866943523 1204 36-37 0.01579696582036405 1204 38-39 -0.11762125790082223 1204 40-41 -0.1770873697367108 1204 42-43 -0.01822904771997713 1204 44-45 -0.08843569032118381 1204 46-47 -0.21033897118931577 1204 48-49 -0.0627259564269167 1204 50-51 -0.2160009977725892 1204 52-53 -0.2595959740991276 1204 54-55 -0.2397262107568352 1204 56-57 -0.270492528913465 1204 58-59 0.0671483299561757 1204 60-61 -0.12612243932954925 1204 62-63 -0.14894962353967856 1204 64-65 -0.2603204994653865 1204 66-67 -0.03952274759980412 1204 68-69 -0.15569988641832566 1204 70-71 -0.3560287013377348 1204 72-73 -0.3243311257089019 1204 74-75 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0.06059453273813631 2401 42-43 0.1648725942796503 2401 44-45 0.04692360138766816 2401 46-47 0.013249891688317916 2401 48-49 0.037667290703559786 2401 50-51 0.062281916161062156 2401 52-53 0.08106844749332254 2401 54-55 -0.04196784112505014 2401 56-57 0.07527294118565919 2401 58-59 -0.15866631645203455 2401 60-61 0.2523229757851482 2401 62-63 0.11365183027906767 2401 64-65 -0.021748977254254953 2401 66-67 0.1578250737121465 2401 68-69 -0.13155495174620313 2401 70-71 0.10163123000713625 2401 72-73 0.26038876691772117 2401 74-75 0.4202753720706909 2401 76-77 0.3577311139116901 2401 78-79 0.12053419638570517 2401 80-81 0.3854106778607189 2401 82-83 0.100597356644343 2401 84-85 0.36218988374012184 2401 86-87 0.29119315035888604 2401 88-89 0.4888928227046634 2401 90-91 -0.579228795585216 2402 1 0.04730840087492538 2402 2 0.08994727893603027 2402 3 -0.014988664971177457 2402 4 0.10805750935150371 2402 5 0.011741711692906165 2402 6 -0.028031250329526358 2402 7 0.024503911979891768 2402 8 0.03415023579997012 2402 9 0.007788808377007683 2402 10-11 0.04995723825191334 2402 12-13 0.13807571052715417 2402 14-15 0.06685707943348973 2402 16-17 0.0029610240838593427 2402 18-19 0.022052685279028594 2402 20-21 -0.03217997641510806 2402 22-23 0.013107701111401582 2402 24-25 0.03596974591273039 2402 26-27 0.0037305200985500164 2402 28-29 0.08957070612174078 2402 30-31 0.0048387349331378005 2402 32-33 0.019780573019588132 2402 34-35 0.09110430105119605 2402 36-37 0.04604510241164661 2402 38-39 -0.4436428586810237 2402 40-41 -0.004585574305423279 2402 42-43 -0.03594109527639233 2402 44-45 0.09603250277023534 2402 46-47 0.06642878543718211 2402 48-49 0.09583189304461115 2402 50-51 7.360249639773997E-4 2402 52-53 0.00663364523744292 2402 54-55 0.026097924247061144 2402 56-57 -0.033462809225738965 2402 58-59 0.08214033979589175 2402 60-61 0.03870776029111056 2402 62-63 0.21443036931454884 2402 64-65 0.13008343636744257 2402 66-67 0.2646344156140259 2402 68-69 0.23432679822443703 2402 70-71 0.05871875133363602 2402 72-73 -0.037954059346596125 2402 74-75 -0.05545716613961815 2402 76-77 -0.0027996002344607973 2402 78-79 0.20992447435249062 2402 80-81 -0.11058121025651957 2402 82-83 -0.11589871546870967 2402 84-85 0.15113412251604075 2402 86-87 0.45433040526084767 2402 88-89 0.3028463110767561 2402 90-91 0.5636283472719228 2403 1 0.010763457614920924 2403 2 0.04504590082230919 2403 3 0.022215751906564662 2403 4 0.07348699819296911 2403 5 0.2614761273075388 2403 6 0.04115497784037103 2403 7 0.06685248899271556 2403 8 0.09408654873083577 2403 9 0.04824671707447692 2403 10-11 0.15463870318745876 2403 12-13 0.1372792897670294 2403 14-15 0.11358929458675959 2403 16-17 0.016031498678842127 2403 18-19 0.09012339255119173 2403 20-21 0.06288660249902733 2403 22-23 0.08204463229878911 2403 24-25 0.04097297015370316 2403 26-27 0.06305655401623511 2403 28-29 0.16127184225118185 2403 30-31 0.17091174238545648 2403 32-33 0.07251564064734595 2403 34-35 0.05075100565105117 2403 36-37 -0.016010026931063237 2403 38-39 0.13436550180156814 2403 40-41 0.10841524763460342 2403 42-43 0.04400527911348462 2403 44-45 0.1327171878988409 2403 46-47 0.17844813271050697 2403 48-49 0.06752195267984007 2403 50-51 0.14392026030783 2403 52-53 -0.013315582251877345 2403 54-55 -0.03238663160174582 2403 56-57 0.1904787454224035 2403 58-59 0.11667810067662288 2403 60-61 0.10601087345157367 2403 62-63 0.19517056716549064 2403 64-65 -0.029591213801396066 2403 66-67 0.036618312535779296 2403 68-69 0.13018774228412866 2403 70-71 0.2884031978918742 2403 72-73 0.17224195373117368 2403 74-75 0.19569468677806867 2403 76-77 0.268502495724924 2403 78-79 0.14427342686921207 2403 80-81 0.2892050200250651 2403 82-83 0.3446303024939823 2403 84-85 0.2755649028037155 2403 86-87 0.6111931503588863 2403 88-89 -0.1521328183209718 2403 90-91 0.5636283472719228 2404 1 0.031447307376417655 2404 2 -0.14293181192872595 2404 3 0.06681269743107521 2404 4 -0.07602677099771427 2404 5 0.031160658513698536 2404 6 0.008371877668004402 2404 7 -0.03809960945599755 2404 8 0.024829323413165127 2404 9 0.0458768930475415 2404 10-11 0.04543920024121917 2404 12-13 0.07518605210318441 2404 14-15 0.06402822511948614 2404 16-17 0.10429221138979017 2404 18-19 -0.02062659742756523 2404 20-21 -0.03168508948798632 2404 22-23 0.001464201786099295 2404 24-25 0.09332395423997042 2404 26-27 0.06888651353040132 2404 28-29 0.0165137551064376 2404 30-31 0.02529102646771264 2404 32-33 0.026880247114526412 2404 34-35 0.08912338368013906 2404 36-37 0.05678864056537236 2404 38-39 0.11761346339343959 2404 40-41 0.05715421407911947 2404 42-43 0.1912598426925527 2404 44-45 0.07907167909839785 2404 46-47 0.0828707402117459 2404 48-49 0.005536013060364553 2404 50-51 0.052225279188000684 2404 52-53 -0.08182946975384198 2404 54-55 -0.0755494605339706 2404 56-57 0.03900820236437852 2404 58-59 -0.06553092241193781 2404 60-61 -0.06929664799507407 2404 62-63 -0.1804599920613157 2404 64-65 0.11679299476845983 2404 66-67 -0.11103841413671489 2404 68-69 0.04490544140480068 2404 70-71 0.03423118248750967 2404 72-73 0.11506656220437605 2404 74-75 -0.09024307424705569 2404 76-77 -0.21853125427710296 2404 78-79 7.01527121677259E-4 2404 80-81 -0.14615516897905678 2404 82-83 -0.08270357694818387 2404 84-85 -0.38730245869625435 2404 86-87 -0.97882467495306 2404 88-89 0.4888928227046634 2404 90-91 0.5636283472719228 >>END_MODULE >>Per sequence quality scores pass #Quality Count 29 1.0 30 19.0 31 424.0 32 3044.0 33 5313.0 34 7178.0 35 8824.0 36 12467.0 37 18527.0 38 46660.0 39 268990.0 40 318163.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.566856629109207 14.895375647104887 25.758472179927782 29.779295543858126 2 32.07767722473605 16.18531732219515 27.705360250609118 24.031645202459682 3 29.435188004814318 18.874146256579806 22.090601934426704 29.60006380417917 4 26.346195675816762 21.171096706834298 21.41543771117008 31.067269906178858 5 25.69336291527095 21.74446426240919 22.93223706152753 29.629935760792332 6 25.537187685793423 23.89031481562042 24.806484824756023 25.766012673830136 7 25.063876683922796 23.801569002769682 23.988631255347226 27.145923057960296 8 24.620727657661575 24.524296341410363 24.10956917678108 26.745406824146983 9 24.413364075346937 24.62826815156393 24.81721552761706 26.141152245472078 10-11 24.871884108409102 23.739359928075288 24.214193529676194 27.174562433839416 12-13 24.746523397282523 23.166862429489132 24.321790577282812 27.764823595945536 14-15 24.968387929409378 23.089354852742854 24.221444004582303 27.720813213265473 16-17 25.340119777845448 23.11908179985789 23.727034120734906 27.81376430156175 18-19 25.220631951392818 23.360015080987807 23.76321399051638 27.656138977103 20-21 25.308217688258583 23.348414321138034 23.80671683995302 27.536651150650364 22-23 25.36332129754499 22.851611780571627 23.729934310697352 28.055132611186036 24-25 25.384855208016127 23.077174054900595 23.75755862008962 27.780412116993663 26-27 25.500572787517584 23.401705311697913 23.71282318991894 27.384898710865563 28-29 25.76122736039211 23.135250358898507 23.50306695088528 27.600455329824104 30-31 25.622540219317454 23.250492318668666 23.690098811764603 27.43686865024928 32-33 25.580794043703552 23.241522992574005 23.88554158513668 27.292141378585764 34-35 25.74066538706618 23.003321411315827 23.777539354717238 27.47847384690075 36-37 25.63322894540891 23.32377923059847 23.869911293473695 27.173080530518927 38-39 25.7579737270564 23.344199217934623 23.86143040035624 27.036396654652734 40-41 25.70212109439623 23.115021645906793 23.890798305503765 27.29205895419321 42-43 25.56072627785105 23.351469249557617 24.012714168546854 27.07509030404448 44-45 25.678561066964544 23.418411310310955 24.05386110727779 26.84916651544671 46-47 25.660309754147182 23.166425820596967 24.056963061568798 27.116301363687057 48-49 25.759055530853153 23.346898646120064 24.096142035474966 26.797903787551814 50-51 25.654438944251094 23.42260939740458 24.160451928550327 26.762499729793994 52-53 25.70065106480392 23.257277053927762 24.193452852630752 26.848619028637565 54-55 25.588066449318 23.415175896831514 24.24176850041938 26.754989153431108 56-57 25.708513012273453 23.38309057828976 24.236502719057526 26.67189369037926 58-59 25.716599704625935 23.350658012645276 24.183467673328085 26.749274609400697 60-61 25.883437836338274 23.51356141617198 24.087150625605247 26.515850121884498 62-63 25.849522372169474 23.427162487057544 24.235005761952394 26.488309378820585 64-65 25.83413764444087 23.35183248880667 24.134025040885813 26.680004825866643 66-67 25.682939742088568 23.418775674244795 24.21020902651379 26.688075557152853 68-69 25.788829784850346 23.273895683114716 24.584690809197525 26.352583722837412 70-71 25.812218534887254 23.311995721992417 24.125655987375684 26.750129755744638 72-73 25.938478063596126 23.51735590248841 24.02047027303986 26.523695760875604 74-75 26.154703097286756 23.47737500183228 23.89147036836165 26.476451532519313 76-77 26.127135009302776 23.2975036814306 24.0276173428448 26.547743966421827 78-79 26.18522305332831 23.483829306355272 23.673249744390034 26.657697895926386 80-81 26.181876770633334 23.434793700237652 23.746290900790825 26.63703862833819 82-83 26.229989465867686 23.033484725508142 23.639629060389936 27.09689674823424 84-85 26.362983387010818 22.648810213396974 23.547811580435134 27.44039481915707 86-87 27.113280592492845 22.491163103854568 22.659484935196094 27.73607136845649 88-89 26.360298208523425 21.525536886613995 23.767664404139314 28.346500500723266 90-91 22.108797850906647 21.9341840161182 25.54734721289456 30.40967092008059 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 2.5 9 7.5 10 13.5 11 22.0 12 25.5 13 25.5 14 42.5 15 70.0 16 104.0 17 155.5 18 218.5 19 304.0 20 441.5 21 622.5 22 830.0 23 1108.0 24 1487.5 25 1901.5 26 2412.5 27 3073.0 28 3892.0 29 4875.5 30 5991.5 31 7070.5 32 8239.5 33 9579.5 34 11211.0 35 13131.0 36 15175.5 37 17249.5 38 19616.5 39 22320.5 40 24842.0 41 27334.5 42 29830.5 43 32305.5 44 34737.0 45 36998.5 46 39173.5 47 41351.5 48 43201.0 49 44778.0 50 46256.0 51 47117.0 52 47536.0 53 47998.0 54 48366.0 55 48258.0 56 47632.0 57 46761.5 58 45514.5 59 44092.5 60 42557.0 61 40629.5 62 38833.5 63 36935.0 64 34433.5 65 31785.0 66 29070.0 67 26494.5 68 23934.5 69 21211.5 70 18493.0 71 15938.5 72 13673.0 73 11470.0 74 9474.0 75 7934.5 76 6553.0 77 5211.0 78 4165.0 79 3295.0 80 2577.5 81 2082.5 82 1654.5 83 1247.0 84 933.0 85 711.5 86 535.0 87 400.5 88 301.5 89 213.0 90 157.0 91 126.0 92 90.0 93 46.5 94 27.0 95 20.0 96 13.0 97 9.5 98 7.5 99 3.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01305085483099143 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0611918503581114 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.9248403768364345E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 7.3290678158645E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 12096.0 32-33 27174.0 34-35 31477.0 36-37 34473.0 38-39 36879.0 40-41 38476.0 42-43 39144.0 44-45 38869.0 46-47 38313.0 48-49 36814.0 50-51 35138.0 52-53 33128.0 54-55 31587.0 56-57 28836.0 58-59 26513.0 60-61 23920.0 62-63 22468.0 64-65 20189.0 66-67 18289.0 68-69 16680.0 70-71 14750.0 72-73 13107.0 74-75 11899.0 76-77 10529.0 78-79 9473.0 80-81 8491.0 82-83 7527.0 84-85 6926.0 86-87 6081.0 88-89 5356.0 90-91 5008.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.92715022441793 #Duplication Level Percentage of deduplicated Percentage of total 1 99.93423360901198 99.86143174409816 2 0.05987644620353518 0.11966565269369876 3 0.004643395386681103 0.013920038050687552 4 0.0012465493978029178 0.004982565157456395 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.002610170966198286 0.0 0.0 0.0 0.0 2 0.002610170966198286 0.0 0.0 0.0 0.0 3 0.002610170966198286 0.0 0.0 0.0 2.90018996244254E-4 4 0.002755180464320413 0.0 0.0 0.0 2.90018996244254E-4 5 0.00290018996244254 0.0 0.0 0.0 2.90018996244254E-4 6 0.003045199460564667 0.0 0.0 0.0 2.90018996244254E-4 7 0.003335218456808921 0.0 0.0 0.0 2.90018996244254E-4 8 0.010875712359159524 0.0 0.0 0.0 2.90018996244254E-4 9 0.011020721857281653 0.0 0.0 0.0 2.90018996244254E-4 10-11 0.011020721857281653 0.0 0.0 0.0 3.625237453053175E-4 12-13 0.011020721857281653 0.0 0.0 0.0 4.35028494366381E-4 14-15 0.011020721857281653 0.0 0.0 0.0 5.80037992488508E-4 16-17 0.011310740853525906 0.0 0.0 0.0 5.80037992488508E-4 18-19 0.011455750351648033 0.0 0.0 0.0 5.80037992488508E-4 20-21 0.011528255100709095 0.0 0.0 0.0 5.80037992488508E-4 22-23 0.01160075984977016 0.0 0.0 0.0 5.80037992488508E-4 24-25 0.011890778846014414 0.0 0.0 0.0 5.80037992488508E-4 26-27 0.012325807340380794 0.0 0.0 0.0 5.80037992488508E-4 28-29 0.012543321587563987 0.0 0.0 0.0 5.80037992488508E-4 30-31 0.013195864329113557 0.0 0.0 0.0 5.80037992488508E-4 32-33 0.013920911819724192 0.0 0.0 0.0 5.80037992488508E-4 34-35 0.014355940314090572 0.0 0.0 0.0 5.80037992488508E-4 36-37 0.015153492553762271 0.0 2.175142471831905E-4 0.0 5.80037992488508E-4 38-39 0.01573353054625078 0.0 2.90018996244254E-4 0.0 5.80037992488508E-4 40-41 0.01595104479343397 0.0 2.90018996244254E-4 0.0 6.525427415495714E-4 42-43 0.01660358753498354 0.0 2.90018996244254E-4 0.0 7.25047490610635E-4 44-45 0.017038616029349925 0.0 2.90018996244254E-4 0.0 7.25047490610635E-4 46-47 0.017328635025594175 0.0 2.90018996244254E-4 0.0 7.25047490610635E-4 48-49 0.01776366351996056 0.0 2.90018996244254E-4 0.0 7.25047490610635E-4 50-51 0.018126187265265874 0.0 2.90018996244254E-4 0.0 7.25047490610635E-4 52-53 0.018271196763388002 0.0 2.90018996244254E-4 0.0 7.25047490610635E-4 54-55 0.018488711010571195 0.0 2.90018996244254E-4 0.0 7.25047490610635E-4 56-57 0.01870622525775438 0.0 2.90018996244254E-4 0.0 7.25047490610635E-4 58-59 0.018923739504937573 0.0 2.90018996244254E-4 0.0 7.25047490610635E-4 60-61 0.019431272748365015 0.0 2.90018996244254E-4 0.0 7.25047490610635E-4 62-63 0.019721291744609272 0.0 2.90018996244254E-4 0.0 7.25047490610635E-4 64-65 0.019721291744609272 0.0 2.90018996244254E-4 0.0 7.25047490610635E-4 66-67 0.019938805991792465 0.0 2.90018996244254E-4 0.0 7.25047490610635E-4 68-69 0.02008381548991459 0.0 2.90018996244254E-4 0.0 7.25047490610635E-4 70-71 0.020156320238975654 0.0 2.90018996244254E-4 0.0 7.25047490610635E-4 72-73 0.02030132973709778 0.0 2.90018996244254E-4 0.0 7.25047490610635E-4 74-75 0.02030132973709778 0.0 2.90018996244254E-4 0.0 7.25047490610635E-4 76-77 0.02030132973709778 0.0 2.90018996244254E-4 0.0 7.25047490610635E-4 78-79 0.020446339235219907 0.0 2.90018996244254E-4 0.0 7.25047490610635E-4 80 0.020446339235219907 0.0 2.90018996244254E-4 0.0 7.25047490610635E-4 >>END_MODULE