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"CACGA":45320, "CACGT":398918, "CACGC":44000, "CACGG":41231, "CAGAA":71050, "CAGAT":269922, "CAGAC":40246, "CAGAG":51982, "CAGTA":33244, "CAGTT":48757, "CAGTC":107892, "CAGTG":26889, "CAGCA":98559, "CAGCT":58168, "CAGCC":83666, "CAGCG":52202, "CAGGA":91797, "CAGGT":54078, "CAGGC":62814, "CAGGG":52690, "CTAAA":36903, "CTAAT":21217, "CTAAC":25243, "CTAAG":26392, "CTATA":20844, "CTATT":33037, "CTATC":35788, "CTATG":26870, "CTACA":37490, "CTACT":30531, "CTACC":44778, "CTACG":32876, "CTAGA":73874, "CTAGT":15864, "CTAGC":20716, "CTAGG":16379, "CTTAA":24112, "CTTAT":34918, "CTTAC":23418, "CTTAG":34603, "CTTTA":52514, "CTTTT":92298, "CTTTC":76764, "CTTTG":68449, "CTTCA":92928, "CTTCT":101896, "CTTCC":87227, "CTTCG":64309, "CTTGA":63575, "CTTGT":60931, "CTTGC":57767, "CTTGG":70789, "CTCAA":57897, "CTCAT":43907, "CTCAC":43172, "CTCAG":54381, "CTCTA":31558, "CTCTT":66129, "CTCTC":64309, "CTCTG":47533, "CTCCA":207479, "CTCCT":127735, "CTCCC":91310, "CTCCG":58449, "CTCGA":50143, "CTCGT":45124, "CTCGC":48905, "CTCGG":51718, "CTGAA":298707, "CTGAT":48435, "CTGAC":36466, "CTGAG":47987, "CTGTA":29302, "CTGTT":66820, "CTGTC":37506, "CTGTG":38934, "CTGCA":62444, "CTGCT":105878, "CTGCC":79597, "CTGCG":69471, "CTGGA":75330, "CTGGT":73581, "CTGGC":67881, "CTGGG":72978, "CCAAA":108303, "CCAAT":54353, "CCAAC":103886, "CCAAG":86008, "CCATA":45879, "CCATT":54979, "CCATC":78201, "CCATG":52378, "CCACA":80130, "CCACT":39326, "CCACC":148358, "CCACG":51673, "CCAGA":143041, "CCAGT":137179, "CCAGC":101243, "CCAGG":68411, "CCTAA":33073, "CCTAT":27989, "CCTAC":34407, "CCTAG":35443, "CCTTA":30092, "CCTTT":76965, "CCTTC":91145, "CCTTG":71359, "CCTCA":69295, "CCTCT":65695, "CCTCC":146854, "CCTCG":63803, "CCTGA":58966, "CCTGT":56904, "CCTGC":106814, "CCTGG":70758, "CCCAA":100233, "CCCAT":57963, "CCCAC":94144, "CCCAG":92288, "CCCTA":36793, "CCCTT":60460, "CCCTC":89138, "CCCTG":61508, "CCCCA":104852, "CCCCT":69713, "CCCCC":93128, "CCCCG":66883, "CCCGA":54444, "CCCGT":40460, "CCCGC":82593, "CCCGG":58918, "CCGAA":62639, "CCGAT":48266, "CCGAC":52010, "CCGAG":65023, "CCGTA":35586, "CCGTT":44165, "CCGTC":54724, "CCGTG":41410, "CCGCA":64590, "CCGCT":56645, "CCGCC":126359, "CCGCG":62854, "CCGGA":58664, "CCGGT":53798, "CCGGC":78253, "CCGGG":57139, "CGAAA":63245, "CGAAT":42405, "CGAAC":46504, "CGAAG":71515, "CGATA":38302, "CGATT":49521, "CGATC":50984, "CGATG":58301, "CGACA":44665, "CGACT":35268, "CGACC":60339, "CGACG":58083, "CGAGA":84764, "CGAGT":28863, "CGAGC":49234, "CGAGG":57892, "CGTAA":42218, "CGTAT":21786, "CGTAC":22144, "CGTAG":43055, "CGTTA":24065, "CGTTT":44914, "CGTTC":46260, "CGTTG":47085, "CGTCA":43665, "CGTCT":354535, "CGTCC":48263, "CGTCG":56519, "CGTGA":40785, "CGTGT":26627, "CGTGC":33866, "CGTGG":47363, "CGCAA":48481, "CGCAT":34349, "CGCAC":43087, "CGCAG":59522, "CGCTA":22764, "CGCTT":46668, "CGCTC":47557, "CGCTG":56827, "CGCCA":83214, "CGCCT":69243, "CGCCC":84385, "CGCCG":101312, "CGCGA":42166, "CGCGT":30969, "CGCGC":56813, "CGCGG":61775, "CGGAA":698750, "CGGAT":44739, "CGGAC":33796, "CGGAG":62838, "CGGTA":35809, "CGGTT":50017, "CGGTC":50884, "CGGTG":65112, "CGGCA":70586, "CGGCT":65752, "CGGCC":68362, "CGGCG":94348, "CGGGA":51744, "CGGGT":46517, "CGGGC":55046, "CGGGG":57996, "GAAAA":106228, "GAAAT":70911, "GAAAC":65608, "GAAAG":80466, "GAATA":47341, "GAATT":57389, "GAATC":51516, "GAATG":44233, "GAACA":60423, "GAACT":233455, "GAACC":61847, "GAACG":43840, "GAAGA":734983, "GAAGT":50265, "GAAGC":75581, "GAAGG":75875, "GATAA":49672, "GATAT":42099, "GATAC":28843, "GATAG":50944, "GATTA":39598, "GATTT":77390, "GATTC":54540, "GATTG":56421, "GATCA":56770, "GATCT":55066, "GATCC":49594, "GATCG":756418, "GATGA":83938, "GATGT":44692, "GATGC":49104, "GATGG":70311, "GACAA":56025, "GACAT":37576, "GACAC":30501, "GACAG":48075, "GACTA":24090, "GACTT":46312, "GACTC":27743, "GACTG":34829, "GACCA":62504, "GACCT":46858, "GACCC":51624, "GACCG":49171, "GACGA":61719, "GACGT":28611, "GACGC":41111, "GACGG":48169, "GAGAA":80952, "GAGAT":205201, "GAGAC":49393, "GAGAG":64057, "GAGTA":26204, "GAGTT":43311, "GAGTC":25523, "GAGTG":28241, "GAGCA":570785, "GAGCT":39836, "GAGCC":50140, "GAGCG":40836, "GAGGA":112580, "GAGGT":51048, "GAGGC":57432, "GAGGG":62569, "GTAAA":34534, "GTAAT":29326, "GTAAC":36750, "GTAAG":30490, "GTATA":19180, "GTATT":33833, "GTATC":28180, "GTATG":28190, "GTACA":27432, "GTACT":23871, "GTACC":28031, "GTACG":21977, "GTAGA":94111, "GTAGT":27894, "GTAGC":33906, "GTAGG":29430, "GTTAA":24544, "GTTAT":28633, "GTTAC":27710, "GTTAG":38419, "GTTTA":39254, "GTTTT":84532, "GTTTC":68007, "GTTTG":73826, "GTTCA":52293, "GTTCT":55121, "GTTCC":63052, "GTTCG":43317, "GTTGA":64053, "GTTGT":72031, "GTTGC":53335, "GTTGG":89629, "GTCAA":45901, "GTCAT":39682, "GTCAC":79940, "GTCAG":47341, "GTCTA":28102, "GTCTT":50617, "GTCTC":49769, "GTCTG":327541, "GTCCA":54468, "GTCCT":46446, "GTCCC":41810, "GTCCG":33105, "GTCGA":49834, "GTCGT":44638, "GTCGC":46364, "GTCGG":47976, "GTGAA":62807, "GTGAT":47294, "GTGAC":30849, "GTGAG":46154, "GTGTA":21567, "GTGTT":49734, "GTGTC":28495, "GTGTG":42216, "GTGCA":34968, "GTGCT":47232, "GTGCC":40983, "GTGCG":36119, "GTGGA":60772, "GTGGT":82437, "GTGGC":57022, "GTGGG":74356, "GCAAA":61465, "GCAAT":44710, "GCAAC":53544, "GCAAG":66500, "GCATA":34352, "GCATT":44304, "GCATC":48911, "GCATG":37813, "GCACA":495861, "GCACT":25922, "GCACC":68040, "GCACG":33990, "GCAGA":118335, "GCAGT":37061, "GCAGC":80037, "GCAGG":88465, "GCTAA":24401, "GCTAT":28242, "GCTAC":35970, "GCTAG":36601, "GCTTA":30974, "GCTTT":65305, "GCTTC":84101, "GCTTG":72321, "GCTCA":48245, "GCTCT":43291, "GCTCC":66628, "GCTCG":49149, "GCTGA":62093, "GCTGT":49823, "GCTGC":82344, "GCTGG":94718, "GCCAA":77837, "GCCAT":52002, "GCCAC":62659, "GCCAG":88139, "GCCTA":29677, "GCCTT":57613, "GCCTC":65047, "GCCTG":69006, "GCCCA":75922, "GCCCT":59408, "GCCCC":67645, "GCCCG":58068, "GCCGA":75549, "GCCGT":57446, "GCCGC":95463, "GCCGG":76694, "GCGAA":50898, "GCGAT":49396, "GCGAC":45718, "GCGAG":54172, "GCGTA":37008, "GCGTT":40465, "GCGTC":39433, "GCGTG":38872, "GCGCA":46391, "GCGCT":42604, "GCGCC":67004, "GCGCG":53745, "GCGGA":58375, "GCGGT":61653, "GCGGC":86819, "GCGGG":68725, "GGAAA":86047, "GGAAT":58580, "GGAAC":57761, "GGAAG":701761, "GGATA":48835, "GGATT":66815, "GGATC":46776, "GGATG":63797, "GGACA":48511, "GGACT":38023, "GGACC":38956, "GGACG":44434, "GGAGA":144247, "GGAGT":39447, "GGAGC":57401, "GGAGG":112056, "GGTAA":35043, "GGTAT":32712, "GGTAC":28211, "GGTAG":56088, "GGTTA":35539, "GGTTT":88634, "GGTTC":57153, "GGTTG":97651, "GGTCA":52218, "GGTCT":55181, "GGTCC":38823, "GGTCG":54775, "GGTGA":75301, "GGTGT":58739, "GGTGC":59835, "GGTGG":121096, "GGCAA":66244, "GGCAT":51049, "GGCAC":40473, "GGCAG":87820, "GGCTA":36597, "GGCTT":76213, "GGCTC":50464, "GGCTG":78846, "GGCCA":61860, "GGCCT":50091, "GGCCC":44981, "GGCCG":67144, "GGCGA":80515, "GGCGT":50092, "GGCGC":64391, "GGCGG":106894, "GGGAA":60716, "GGGAT":55937, "GGGAC":39710, "GGGAG":82076, "GGGTA":32460, "GGGTT":80899, "GGGTC":46687, "GGGTG":76132, "GGGCA":63440, "GGGCT":56361, "GGGCC":43336, "GGGCG":70126, "GGGGA":67651, "GGGGT":70160, "GGGGC":56821, "GGGGG":125313 }, "overrepresented_sequences": { } }, "read2_before_filtering": { "total_reads": 1172342, "total_bases": 71512862, "q20_bases": 69281236, "q30_bases": 65804669, "total_cycles": 61, "quality_curves": { "A":[39.2971,39.1458,39.1853,38.9531,39.0947,39.1142,39.2168,39.1592,39.1774,39.1763,39.1599,39.1786,39.1941,39.1866,39.1202,39.1144,39.1949,39.1544,39.2267,39.1113,38.5113,39.1139,39.1504,39.1685,38.7085,39.1081,39.1324,38.5644,39.028,38.3194,39.0577,39.1711,39.0697,39.0318,38.9986,39.0585,39.0614,39.089,39.0172,38.7682,38.9298,38.8134,38.8108,38.6258,38.6024,38.7758,38.733,37.9467,38.2986,37.4449,37.1459,36.7045,37.6392,37.3198,36.8262,35.306,34.9725,34.3394,33.8866,34.3005,33.2932], 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"G":[39.2003,39.1665,39.1369,39.1104,39.1777,39.1452,39.1951,39.1935,39.2415,39.2363,39.242,39.2316,39.2014,39.2556,39.2104,39.2256,39.2678,39.1898,39.2704,38.6361,39.3225,39.0071,39.249,39.2535,38.6669,39.104,39.1659,38.608,39.0243,38.4501,39.1175,38.8993,39,38.9942,38.8617,38.9445,38.6834,39.0636,38.5809,39.0023,38.9312,38.375,38.7717,38.0138,38.5322,38.4408,38.0475,38.1645,37.7559,37.7593,37.4368,37.3205,36.8671,36.8118,36.2636,35.3512,35.2627,35.7599,35.4043,35.6484,34.5533], "mean":[39.3242,39.2386,39.1915,39.055,39.0933,39.1997,39.212,39.2042,39.2301,39.2457,39.2303,39.238,39.1209,39.2673,39.2136,39.2325,39.2611,39.2087,39.2738,38.915,39.1281,39.1213,39.2307,39.2434,39.0288,39.1907,39.2063,38.962,39.1602,38.8509,39.0004,39.0669,39.0661,38.9441,38.8995,38.9905,38.9822,38.902,38.9191,38.6853,38.7843,38.5469,38.8007,38.3558,38.6088,38.2237,37.7195,38.0574,37.6917,37.8424,37.4486,37.3912,36.6395,35.933,35.7424,35.4636,35.5164,35.299,34.7852,34.0576,33.8104] }, "content_curves": { 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"CCTGG":62439, "CCCAA":87133, "CCCAT":52010, "CCCAC":83215, "CCCAG":62920, "CCCTA":28467, "CCCTT":54161, "CCCTC":78405, "CCCTG":55004, "CCCCA":87124, "CCCCT":61599, "CCCCC":74351, "CCCCG":58703, "CCCGA":44870, "CCCGT":34856, "CCCGC":71729, "CCCGG":51221, "CCGAA":51827, "CCGAT":42444, "CCGAC":45008, "CCGAG":48145, "CCGTA":28289, "CCGTT":39949, "CCGTC":46899, "CCGTG":36320, "CCGCA":51219, "CCGCT":49784, "CCGCC":109740, "CCGCG":54825, "CCGGA":48310, "CCGGT":46673, "CCGGC":67390, "CCGGG":49252, "CGAAA":53041, "CGAAT":38544, "CGAAC":39736, "CGAAG":51900, "CGATA":30959, "CGATT":44585, "CGATC":43850, "CGATG":51329, "CGACA":35903, "CGACT":31465, "CGACC":52918, "CGACG":50087, "CGAGA":44761, "CGAGT":25773, "CGAGC":42254, "CGAGG":49763, "CGTAA":25457, "CGTAT":19609, "CGTAC":19410, "CGTAG":27791, "CGTTA":18791, "CGTTT":40499, "CGTTC":40970, "CGTTG":41885, "CGTCA":34509, "CGTCT":35429, "CGTCC":42443, "CGTCG":48600, "CGTGA":34620, "CGTGT":23143, "CGTGC":29330, "CGTGG":41675, "CGCAA":40599, "CGCAT":30547, "CGCAC":36361, "CGCAG":39364, "CGCTA":17071, "CGCTT":41274, "CGCTC":40937, "CGCTG":48673, "CGCCA":66877, "CGCCT":61118, "CGCCC":73381, "CGCCG":86626, "CGCGA":34240, "CGCGT":27124, "CGCGC":48576, "CGCGG":53650, "CGGAA":50340, "CGGAT":39846, "CGGAC":29216, "CGGAG":45555, "CGGTA":27245, "CGGTT":45065, "CGGTC":44798, "CGGTG":56878, "CGGCA":56000, "CGGCT":57791, "CGGCC":58629, "CGGCG":79942, "CGGGA":42639, "CGGGT":41149, "CGGGC":46950, "CGGGG":49798, "GAAAA":93028, "GAAAT":65599, "GAAAC":60156, "GAAAG":60195, "GAATA":40611, "GAATT":53483, "GAATC":47292, "GAATG":40521, "GAACA":49510, "GAACT":43125, "GAACC":55010, "GAACG":38658, "GAAGA":87067, "GAAGT":45389, "GAAGC":67117, "GAAGG":67049, "GATAA":43006, "GATAT":38931, "GATAC":26250, "GATAG":30464, "GATTA":32776, "GATTT":71914, "GATTC":49978, "GATTG":50723, "GATCA":45639, "GATCT":49454, "GATCC":43851, "GATCG":40536, "GATGA":72705, "GATGT":40150, "GATGC":43525, "GATGG":63312, "GACAA":48597, "GACAT":33563, "GACAC":27001, "GACAG":30049, "GACTA":18404, "GACTT":42342, "GACTC":24625, "GACTG":30295, "GACCA":52233, "GACCT":41683, "GACCC":45871, "GACCG":43212, "GACGA":52168, "GACGT":24819, "GACGC":35314, "GACGG":42373, "GAGAA":69785, "GAGAT":56390, "GAGAC":44530, "GAGAG":47790, "GAGTA":21310, "GAGTT":39059, "GAGTC":22643, "GAGTG":25119, "GAGCA":50890, "GAGCT":34680, "GAGCC":44257, "GAGCG":34582, "GAGGA":95982, "GAGGT":44298, "GAGGC":49923, "GAGGG":54530, "GTAAA":30747, "GTAAT":27076, "GTAAC":23776, "GTAAG":18145, "GTATA":15685, "GTATT":31303, "GTATC":25510, "GTATG":25873, "GTACA":22990, "GTACT":21671, "GTACC":25285, "GTACG":19175, "GTAGA":35088, "GTAGT":25526, "GTAGC":30304, "GTAGG":25615, "GTTAA":20863, "GTTAT":26231, "GTTAC":25503, "GTTAG":19142, "GTTTA":31780, "GTTTT":77618, "GTTTC":62028, "GTTTG":67221, "GTTCA":43962, "GTTCT":49850, "GTTCC":56500, "GTTCG":38067, "GTTGA":55345, "GTTGT":65382, "GTTGC":47803, "GTTGG":81518, "GTCAA":39250, "GTCAT":35577, "GTCAC":28599, "GTCAG":28116, "GTCTA":21462, "GTCTT":45440, "GTCTC":46157, "GTCTG":35794, "GTCCA":45743, "GTCCT":41485, "GTCCC":37714, "GTCCG":28741, "GTCGA":41035, "GTCGT":38934, "GTCGC":40270, "GTCGG":40945, "GTGAA":54538, "GTGAT":41357, "GTGAC":27519, "GTGAG":32866, "GTGTA":16800, "GTGTT":45041, "GTGTC":25226, "GTGTG":36931, "GTGCA":28169, "GTGCT":41530, "GTGCC":36011, "GTGCG":31172, "GTGGA":51167, "GTGGT":74293, "GTGGC":50103, "GTGGG":65023, "GCAAA":51794, "GCAAT":40703, "GCAAC":47466, "GCAAG":47731, "GCATA":27794, "GCATT":40765, "GCATC":43369, "GCATG":33759, "GCACA":33883, "GCACT":23030, "GCACC":59129, "GCACG":29224, "GCAGA":47714, "GCAGT":33422, "GCAGC":71548, "GCAGG":78053, "GCTAA":19550, "GCTAT":25154, "GCTAC":32040, "GCTAG":17938, "GCTTA":24047, "GCTTT":59383, "GCTTC":76187, "GCTTG":65328, "GCTCA":39698, "GCTCT":38120, "GCTCC":59368, "GCTCG":42541, "GCTGA":52679, "GCTGT":43962, "GCTGC":73136, "GCTGG":83094, "GCCAA":65446, "GCCAT":46424, "GCCAC":54974, "GCCAG":56942, "GCCTA":21855, "GCCTT":51411, "GCCTC":58133, "GCCTG":60608, "GCCCA":61390, "GCCCT":52816, "GCCCC":59682, "GCCCG":49810, "GCCGA":62378, "GCCGT":49935, "GCCGC":81275, "GCCGG":65005, "GCGAA":41502, "GCGAT":43331, "GCGAC":39531, "GCGAG":37493, "GCGTA":18279, "GCGTT":35801, "GCGTC":34143, "GCGTG":33805, "GCGCA":36041, "GCGCT":36235, "GCGCC":57856, "GCGCG":45519, "GCGGA":47583, "GCGGT":54606, "GCGGC":74471, "GCGGG":59316, "GGAAA":72488, "GGAAT":54100, "GGAAC":51984, "GGAAG":65559, "GGATA":39950, "GGATT":61340, "GGATC":41703, "GGATG":56913, "GGACA":39503, "GGACT":33492, "GGACC":34556, "GGACG":38094, "GGAGA":82903, "GGAGT":35147, "GGAGC":50581, "GGAGG":97653, "GGTAA":28391, "GGTAT":30063, "GGTAC":25028, "GGTAG":32588, "GGTTA":26773, "GGTTT":81424, "GGTTC":51639, "GGTTG":88380, "GGTCA":41409, "GGTCT":49598, "GGTCC":34447, "GGTCG":47287, "GGTGA":62744, "GGTGT":52851, "GGTGC":52341, "GGTGG":107474, "GGCAA":54863, "GGCAT":45963, "GGCAC":35464, "GGCAG":58390, "GGCTA":28027, "GGCTT":68731, "GGCTC":44933, "GGCTG":69127, "GGCCA":48261, "GGCCT":43641, "GGCCC":38800, "GGCCG":56631, "GGCGA":65478, "GGCGT":43473, "GGCGC":54160, "GGCGG":92177, "GGGAA":50637, "GGGAT":50477, "GGGAC":34080, "GGGAG":59233, "GGGTA":25179, "GGGTT":73494, "GGGTC":41414, "GGGTG":66541, "GGGCA":50890, "GGGCT":50140, "GGGCC":37128, "GGGCG":59948, "GGGGA":55944, "GGGGT":61519, "GGGGC":49565, "GGGGG":53382 }, "overrepresented_sequences": { } }, "command": "/redser4/software/fastp -l 30 -q 25 -y -Y 30 --detect_adapter_for_pe -i /scratch3/cwanket/andy-lake/raw/b2/AM-AL-30A_S29_R1_001.fastq.gz -I /scratch3/cwanket/andy-lake/raw/b2/AM-AL-30A_S29_R2_001.fastq.gz -o AM-AL-30A.trim.R1.fastq.gz -O AM-AL-30A.trim.R2.fastq.gz -m --merged_out AM-AL-30A.trim.merged.fastq.gz " }