##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-2C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 684855 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.24823210752641 40.0 40.0 40.0 40.0 40.0 2 39.12418541151047 40.0 40.0 40.0 40.0 40.0 3 39.106131954939364 40.0 40.0 40.0 40.0 40.0 4 39.06106256068803 40.0 40.0 40.0 40.0 40.0 5 39.20427535755744 40.0 40.0 40.0 40.0 40.0 6 39.24106562703054 40.0 40.0 40.0 40.0 40.0 7 39.2789364171978 40.0 40.0 40.0 40.0 40.0 8 39.30007957888896 40.0 40.0 40.0 40.0 40.0 9 39.33642595877959 40.0 40.0 40.0 40.0 40.0 10-11 39.33374217900139 40.0 40.0 40.0 40.0 40.0 12-13 39.32792780953632 40.0 40.0 40.0 40.0 40.0 14-15 39.34228997379007 40.0 40.0 40.0 40.0 40.0 16-17 39.467154361142136 40.0 40.0 40.0 40.0 40.0 18-19 39.48274890305247 40.0 40.0 40.0 40.0 40.0 20-21 39.49284155040118 40.0 40.0 40.0 40.0 40.0 22-23 39.495636302574994 40.0 40.0 40.0 40.0 40.0 24-25 39.50424250388768 40.0 40.0 40.0 40.0 40.0 26-27 39.488805659592174 40.0 40.0 40.0 40.0 40.0 28-29 39.494584985142836 40.0 40.0 40.0 40.0 40.0 30-31 39.48791383140477 40.0 40.0 40.0 40.0 40.0 32-33 39.48928844394618 40.0 40.0 40.0 40.0 40.0 34-35 39.48975254477246 40.0 40.0 40.0 40.0 40.0 36-37 39.48471877432527 40.0 40.0 40.0 40.0 40.0 38-39 39.47637751750183 40.0 40.0 40.0 40.0 40.0 40-41 39.46504341782739 40.0 40.0 40.0 40.0 40.0 42-43 39.46651326984819 40.0 40.0 40.0 40.0 40.0 44-45 39.4538739964458 40.0 40.0 40.0 40.0 40.0 46-47 39.45288536663495 40.0 40.0 40.0 40.0 40.0 48-49 39.45686588853924 40.0 40.0 40.0 40.0 40.0 50-51 39.45467928129692 40.0 40.0 40.0 40.0 40.0 52-53 39.440848244367174 40.0 40.0 40.0 40.0 40.0 54-55 39.437044288115565 40.0 40.0 40.0 40.0 40.0 56-57 39.43265851891421 40.0 40.0 40.0 40.0 40.0 58-59 39.421251596527526 40.0 40.0 40.0 40.0 40.0 60-61 39.39720521617081 40.0 40.0 40.0 40.0 40.0 62-63 39.35327710515399 40.0 40.0 40.0 40.0 40.0 64-65 39.35075458854168 40.0 40.0 40.0 40.0 40.0 66-67 39.34704950066239 40.0 40.0 40.0 40.0 40.0 68-69 39.34738768658903 40.0 40.0 40.0 40.0 40.0 70-71 39.340231539550935 40.0 40.0 40.0 40.0 40.0 72-73 39.35223954989922 40.0 40.0 40.0 40.0 40.0 74-75 39.334806335007634 40.0 40.0 40.0 40.0 40.0 76-77 39.331556472052114 40.0 40.0 40.0 40.0 40.0 78-79 39.31815942957901 40.0 40.0 40.0 40.0 40.0 80-81 39.315386990301775 40.0 40.0 40.0 40.0 40.0 82-83 39.30084693819887 40.0 40.0 40.0 40.0 40.0 84-85 39.30426998630156 40.0 40.0 40.0 40.0 40.0 86-87 39.31540863927454 40.0 40.0 40.0 40.0 40.0 88-89 39.288297687160664 40.0 40.0 40.0 40.0 40.0 90-91 39.42465343747801 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14145798838102053 1101 2 0.09974257702369727 1101 3 0.20363686901849576 1101 4 0.14833303560896383 1101 5 -0.004728902138744218 1101 6 0.039452222778784574 1101 7 0.0354929462212894 1101 8 0.04837154411718103 1101 9 0.0703700337733082 1101 10-11 -0.05720024612926977 1101 12-13 0.032340330486462676 1101 14-15 0.05834013870065036 1101 16-17 0.03526967807364656 1101 18-19 -0.059826187534412156 1101 20-21 -0.031947870483243435 1101 22-23 0.06894194951875221 1101 24-25 0.13520524928680544 1101 26-27 -0.015582779852230999 1101 28-29 -0.04106067180633488 1101 30-31 0.08696963540609914 1101 32-33 0.16574939457819937 1101 34-35 0.06770050703009645 1101 36-37 0.030801465302474185 1101 38-39 0.10183593561676929 1101 40-41 0.1131469858944456 1101 42-43 0.1300670612762289 1101 44-45 0.09357949852060443 1101 46-47 -0.021987464220963204 1101 48-49 0.10250672008238837 1101 50-51 -0.023232954697405717 1101 52-53 0.10904506882133802 1101 54-55 0.11766370704527418 1101 56-57 0.16986019630434157 1101 58-59 0.22132290346314676 1101 60-61 0.13543940386126962 1101 62-63 -0.006060530119491148 1101 64-65 0.020710472516782374 1101 66-67 0.04524767417834852 1101 68-69 -0.16589450570159414 1101 70-71 -0.11508845395488265 1101 72-73 0.01611379905400412 1101 74-75 -0.0930431128094753 1101 76-77 -9.259516069022311E-4 1101 78-79 -0.08307415716652145 1101 80-81 -0.16604385220799855 1101 82-83 0.06992682202159983 1101 84-85 -0.3164329265282575 1101 86-87 -0.05046272953699571 1101 88-89 0.6377569327058126 1101 90-91 -0.7749193765588771 1102 1 0.20285524637019137 1102 2 0.1365788977851281 1102 3 0.18578416955283217 1102 4 0.07800298678541395 1102 5 -0.02848368465664919 1102 6 0.008770883056783418 1102 7 0.025787901211550945 1102 8 0.13186178042425212 1102 9 -0.01384567397153802 1102 10-11 0.03090792098409878 1102 12-13 -0.04841142064564963 1102 14-15 -0.07362853991752871 1102 16-17 0.04896142593774755 1102 18-19 -0.008912330826547077 1102 20-21 0.05743699075848241 1102 22-23 0.06402908406251129 1102 24-25 0.005709275653281054 1102 26-27 0.005076205857335481 1102 28-29 0.02502231805807753 1102 30-31 -0.035582830756091255 1102 32-33 0.04020091004156967 1102 34-35 0.0361481475569434 1102 36-37 -0.03448614092880575 1102 38-39 0.09523614407383718 1102 40-41 -0.037334930671491406 1102 42-43 0.09962162285899012 1102 44-45 0.012759813290166733 1102 46-47 -0.06486987705715563 1102 48-49 0.07458111126567957 1102 50-51 0.007844013772633218 1102 52-53 -0.006274470154671974 1102 54-55 0.09319919471332838 1102 56-57 0.02849189262595786 1102 58-59 -0.06303684639980389 1102 60-61 -0.06267830124069462 1102 62-63 -0.20880692375151 1102 64-65 0.07435703769274937 1102 66-67 -0.05089272847618531 1102 68-69 -0.05266876418048838 1102 70-71 -0.12337528914785167 1102 72-73 -0.15488419632818307 1102 74-75 -0.15929875359535117 1102 76-77 -0.03219247778825718 1102 78-79 0.09861454196668262 1102 80-81 -0.10409467119801263 1102 82-83 -0.30470228391574494 1102 84-85 -0.19897614869517355 1102 86-87 0.14345668438241432 1102 88-89 0.03458232953120444 1102 90-91 0.5498118062368249 1103 1 0.29205948540737126 1103 2 0.04553344444926921 1103 3 0.05163220436141813 1103 4 0.18322235436569656 1103 5 -0.03211087008808278 1103 6 0.1432570020323709 1103 7 0.060535852582738414 1103 8 0.08950509349165259 1103 9 0.009399572337194684 1103 10-11 0.06072979882730323 1103 12-13 0.035323744964273374 1103 14-15 0.05631147072014642 1103 16-17 -0.00787436004618769 1103 18-19 -0.017958062399330288 1103 20-21 0.12061900385313606 1103 22-23 0.057831070323814515 1103 24-25 0.0853443204356168 1103 26-27 0.12658826520466704 1103 28-29 0.0236533654584008 1103 30-31 0.03529145634600894 1103 32-33 -0.031065229441800568 1103 34-35 -0.028530121969708944 1103 36-37 -0.0309238974590329 1103 38-39 -0.020770532304773326 1103 40-41 0.04234309994033225 1103 42-43 -0.10615082567961309 1103 44-45 0.10395301188214034 1103 46-47 0.017944359355659856 1103 48-49 -0.03668811490936008 1103 50-51 0.001506553206560568 1103 52-53 -0.10118638930050139 1103 54-55 0.022454000602046165 1103 56-57 0.023475605045689463 1103 58-59 -0.06719236245074711 1103 60-61 0.07553167472828903 1103 62-63 -0.07168970651878936 1103 64-65 0.011937784023743347 1103 66-67 0.08531637776290069 1103 68-69 0.12353860745091794 1103 70-71 0.11791753184609632 1103 72-73 0.11307080045629192 1103 74-75 0.03697893113669437 1103 76-77 -0.03656653720876335 1103 78-79 -0.030052678736382177 1103 80-81 0.23300540511485934 1103 82-83 -0.04672022409021537 1103 84-85 0.3279250833423575 1103 86-87 0.39405651051977486 1103 88-89 0.10177019970415557 1103 90-91 -0.25410976239061966 1104 1 0.33962265814520265 1104 2 0.2566470862801893 1104 3 0.14433247102535773 1104 4 0.08201801608636572 1104 5 0.054356574902882926 1104 6 0.13524172899614229 1104 7 0.05524991820971081 1104 8 -0.1140987083888163 1104 9 -0.0923217152248057 1104 10-11 -0.08671079039250884 1104 12-13 -0.11889412612597994 1104 14-15 0.00807460046417674 1104 16-17 -0.038726891306268385 1104 18-19 0.038780960105881945 1104 20-21 0.06725422703703288 1104 22-23 0.01428769008970221 1104 24-25 -0.0799425352331653 1104 26-27 -0.011397526367524335 1104 28-29 -0.06528878228672852 1104 30-31 -0.0866849021813465 1104 32-33 -0.031581219885921996 1104 34-35 0.07873303845359203 1104 36-37 -0.07826523014139752 1104 38-39 -0.08266521480577183 1104 40-41 -0.08597817749314629 1104 42-43 0.05572743265106794 1104 44-45 -0.1587160100805889 1104 46-47 -0.04816063345837307 1104 48-49 -0.05400902891874182 1104 50-51 0.04941704930375579 1104 52-53 -0.011743992601040532 1104 54-55 0.05928328337866873 1104 56-57 0.019152711988866145 1104 58-59 -0.0355097198042742 1104 60-61 0.003243957361846128 1104 62-63 -0.027373460879864808 1104 64-65 0.03232501570984425 1104 66-67 -0.062754957758834 1104 68-69 -0.06698924073084811 1104 70-71 -0.10401962654594854 1104 72-73 -0.31824411602013924 1104 74-75 -0.19109504941855704 1104 76-77 -0.20261170189351674 1104 78-79 -0.028962010024557117 1104 80-81 -0.4148422339717186 1104 82-83 0.1007939025451563 1104 84-85 -0.214091723380335 1104 86-87 -0.23976244338089003 1104 88-89 -0.5085069782639806 1104 90-91 -0.3073310509060292 1201 1 0.3115432711151058 1201 2 0.10523096161431056 1201 3 0.14551257491358882 1201 4 0.19381758900114932 1201 5 0.07764957783970772 1201 6 0.1737575834353322 1201 7 -0.0695808847007271 1201 8 -0.18785397976384388 1201 9 0.04146248457680457 1201 10-11 0.08128664905081706 1201 12-13 0.11987228660252214 1201 14-15 0.03784901248902628 1201 16-17 -0.07202573368054743 1201 18-19 -0.06423013726858073 1201 20-21 0.053604847385635424 1201 22-23 0.04381288762883173 1201 24-25 -0.010544852439231533 1201 26-27 0.05021098210821151 1201 28-29 0.050492114201304616 1201 30-31 0.011486669775671032 1201 32-33 0.039838416098312734 1201 34-35 0.011338234442476391 1201 36-37 -0.09724924893468767 1201 38-39 0.016162687358111327 1201 40-41 -0.053689466392903284 1201 42-43 0.016412191438121226 1201 44-45 0.037031739238322814 1201 46-47 0.0912460087990965 1201 48-49 -0.023801885214211893 1201 50-51 0.025225339751528963 1201 52-53 0.09204080287836547 1201 54-55 0.019170799591336163 1201 56-57 -0.02392444170232011 1201 58-59 0.039010922348268195 1201 60-61 -0.06963976760883384 1201 62-63 -0.10312410541292394 1201 64-65 0.028585852995092864 1201 66-67 -0.016221326152596305 1201 68-69 0.010161948174406632 1201 70-71 -0.06885338788075757 1201 72-73 -0.09118259530041684 1201 74-75 -0.11582433624070632 1201 76-77 -0.10442110965287554 1201 78-79 -2.529195507605664E-4 1201 80-81 0.1230445043190116 1201 82-83 -0.056160725549204926 1201 84-85 0.010496962668796073 1201 86-87 0.32536144628718233 1201 88-89 -0.13370349092852507 1201 90-91 0.32124037766539715 1202 1 0.24452818313922364 1202 2 0.25718523504842494 1202 3 0.04591104074551566 1202 4 0.007729568346590554 1202 5 0.1335840324489297 1202 6 0.0706638768076715 1202 7 0.08283901172800512 1202 8 0.040500669750386464 1202 9 0.04171320465082573 1202 10-11 0.11621195536209683 1202 12-13 0.02363756485704016 1202 14-15 0.1696424072089755 1202 16-17 0.029241298122101966 1202 18-19 -0.03891815493837214 1202 20-21 -0.010619487385362447 1202 22-23 0.014602208147579177 1202 24-25 0.03546765371684302 1202 26-27 0.034019837899393224 1202 28-29 0.005797082338958148 1202 30-31 -0.019094612776896724 1202 32-33 0.12475929213391623 1202 34-35 0.0068549795844319306 1202 36-37 0.08866638179665642 1202 38-39 0.031337114129918575 1202 40-41 0.1018522574525349 1202 42-43 -0.03321680713818154 1202 44-45 0.022598244154188762 1202 46-47 -0.005091682526874308 1202 48-49 0.010438560350451098 1202 50-51 0.07708791215905819 1202 52-53 -0.050623381966609315 1202 54-55 -0.05628564129873581 1202 56-57 0.0960083692165199 1202 58-59 0.1609684260505162 1202 60-61 0.15500040874765375 1202 62-63 -0.05755704228449332 1202 64-65 -0.05265229874832755 1202 66-67 0.08637837410939397 1202 68-69 -0.11195271805015494 1202 70-71 -0.005257134100190797 1202 72-73 0.038922722997945414 1202 74-75 0.11247502236219731 1202 76-77 -0.17432325973955187 1202 78-79 0.10057234579477381 1202 80-81 0.34976228886431215 1202 82-83 0.049611045480190796 1202 84-85 0.16538540080267694 1202 86-87 -0.1274870385612985 1202 88-89 -0.02690773195885754 1202 90-91 0.14981180623682633 1203 1 0.2402813304233149 1203 2 0.1981424789372639 1203 3 0.04021805133917411 1203 4 0.07983685478613012 1203 5 -0.04295732976018485 1203 6 -0.09501972210068743 1203 7 -0.0805321770503511 1203 8 -0.06875073148798805 1203 9 0.008588222991640748 1203 10-11 -0.05662133395328084 1203 12-13 0.009915699669377886 1203 14-15 -0.02244844585907657 1203 16-17 0.0021086279926834095 1203 18-19 0.015110075246212773 1203 20-21 -0.004125958025376519 1203 22-23 -0.07438684605320134 1203 24-25 -0.01857935746371453 1203 26-27 0.002623881682595197 1203 28-29 -6.682755270475127E-4 1203 30-31 -0.017047484215112263 1203 32-33 0.006891536797390074 1203 34-35 -0.014177083750816166 1203 36-37 -0.05369595452015119 1203 38-39 -0.08876232596526279 1203 40-41 -0.08211353622354522 1203 42-43 0.008417601535818164 1203 44-45 0.056001387090653054 1203 46-47 0.06029454267216039 1203 48-49 -0.015299120099669494 1203 50-51 -0.08406936607151749 1203 52-53 -0.09141127654707049 1203 54-55 -0.036156615933457203 1203 56-57 -0.08646787779106546 1203 58-59 -0.21627863874774533 1203 60-61 -0.04415867498408943 1203 62-63 -0.12961219721853467 1203 64-65 0.06371282244910503 1203 66-67 -0.19978098013174161 1203 68-69 -0.04443360928399187 1203 70-71 -0.1532498497509991 1203 72-73 0.12735317008284142 1203 74-75 -0.15199113622008298 1203 76-77 -0.06967999798327185 1203 78-79 0.20081352893578952 1203 80-81 0.30761510747959164 1203 82-83 0.08085572128597107 1203 84-85 -0.26604035671494586 1203 86-87 -0.16139069046495536 1203 88-89 0.07064102974085529 1203 90-91 -0.7634576876072217 1204 1 0.26546491045353093 1204 2 0.13258363715227262 1204 3 -0.11842357414435867 1204 4 -0.03627403335134716 1204 5 -0.18445161292675039 1204 6 0.06357482053828534 1204 7 -0.06244662292833425 1204 8 -0.10605642144975036 1204 9 -0.15622010676741382 1204 10-11 -0.21465030827648235 1204 12-13 -0.14186331412876996 1204 14-15 -0.15630382458601133 1204 16-17 -0.0329537366800281 1204 18-19 -0.18648029137411726 1204 20-21 -0.0589011058626383 1204 22-23 -0.08900492092755741 1204 24-25 -0.05431695902915834 1204 26-27 -0.009536124197026652 1204 28-29 -0.06187367673317823 1204 30-31 -0.08589810585220192 1204 32-33 -0.1446771354932892 1204 34-35 -0.16240588935589528 1204 36-37 -0.13978999957097926 1204 38-39 0.01953704424423819 1204 40-41 -0.2102875961361761 1204 42-43 -0.13625771728405311 1204 44-45 -0.22452587427886783 1204 46-47 -0.036809607068541084 1204 48-49 -0.0692755127021627 1204 50-51 -0.1079741708750106 1204 52-53 -0.1551763904665293 1204 54-55 -0.0678760343055913 1204 56-57 -0.19634769653897166 1204 58-59 -0.08786229670407408 1204 60-61 -0.12630449547215505 1204 62-63 -0.214672255885219 1204 64-65 -0.2539450333581641 1204 66-67 -0.18212912092534594 1204 68-69 -0.215865538567364 1204 70-71 -0.270170976175649 1204 72-73 -0.2705807578765089 1204 74-75 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0.12154926288214796 2401 46-47 0.06063128559576825 2401 48-49 0.08438552621771578 2401 50-51 0.0660740627870382 2401 52-53 0.056556236907070456 2401 54-55 -0.00548684675078448 2401 56-57 0.1675998390208946 2401 58-59 0.05238272522492338 2401 60-61 0.16913434008650086 2401 62-63 0.24507785578642682 2401 64-65 0.0998620923192135 2401 66-67 0.08135055750645392 2401 68-69 -0.0793129565763877 2401 70-71 0.07463696996538971 2401 72-73 0.1529801247046052 2401 74-75 0.12324818500715651 2401 76-77 0.1962560052275819 2401 78-79 0.20055949554736685 2401 80-81 0.2363630763846487 2401 82-83 0.2454966518410373 2401 84-85 0.2545363782668346 2401 86-87 0.06287703804006384 2401 88-89 -0.8402091689891051 2401 90-91 -0.09534948408575161 2402 1 0.41026651098837164 2402 2 0.28336671241724076 2402 3 0.173721474624557 2402 4 0.17830240277167064 2402 5 0.21985047227737198 2402 6 0.11320623069566693 2402 7 0.10802620419752174 2402 8 0.22824040345317798 2402 9 0.14254347329111994 2402 10-11 0.18689664683834195 2402 12-13 0.1983627612181209 2402 14-15 0.17890433404345885 2402 16-17 0.10135955282611064 2402 18-19 0.07253303239671993 2402 20-21 0.08112815169737786 2402 22-23 0.09498402984866061 2402 24-25 0.05159748124508923 2402 26-27 0.12771448241198158 2402 28-29 0.1431453163626557 2402 30-31 0.07985181197427949 2402 32-33 0.09941802876279127 2402 34-35 0.10927912230223313 2402 36-37 0.11799792088147854 2402 38-39 0.09307477930065744 2402 40-41 0.11460453313382146 2402 42-43 0.1427650447148494 2402 44-45 0.11318979326303236 2402 46-47 0.07622070227896671 2402 48-49 0.09192082200286222 2402 50-51 0.013992274145401495 2402 52-53 0.1262216614016296 2402 54-55 0.17685803332430083 2402 56-57 0.11724900979845643 2402 58-59 0.12227646501646205 2402 60-61 0.16713703904882493 2402 62-63 -0.012298219553734668 2402 64-65 0.07740134915214014 2402 66-67 0.19484764538400867 2402 68-69 0.038397418271529205 2402 70-71 0.1289325964417003 2402 72-73 0.1444650387812274 2402 74-75 0.11776980973596096 2402 76-77 0.037850160726961235 2402 78-79 0.17946684990452866 2402 80-81 0.20095523112510705 2402 82-83 -0.01853513601378154 2402 84-85 -0.07135129387519612 2402 86-87 0.06855081505794658 2402 88-89 0.19110232149424178 2402 90-91 -0.19326511684010228 2403 1 -0.3982914313949948 2403 2 -0.24497026374510256 2403 3 -0.056240419642776374 2403 4 -0.108047471300452 2403 5 -0.051536463467236615 2403 6 -0.10250293961652801 2403 7 0.06252179499688992 2403 8 -0.03503285406106471 2403 9 0.10841150549182288 2403 10-11 0.07011679034275886 2403 12-13 0.04581648640091629 2403 14-15 0.038923672037498136 2403 16-17 0.023736951788464467 2403 18-19 0.0773014939222918 2403 20-21 0.02730477232448436 2403 22-23 0.06055158673797223 2403 24-25 0.016586897727385974 2403 26-27 0.012378428092134186 2403 28-29 0.0035977668486424363 2403 30-31 0.09864630333532887 2403 32-33 0.005666860717433053 2403 34-35 0.015486745021718207 2403 36-37 -3.118178105694369E-4 2403 38-39 0.051333447900404394 2403 40-41 0.030433788822513463 2403 42-43 0.05548216125394134 2403 44-45 0.04830624739228284 2403 46-47 0.0537895752058688 2403 48-49 -0.01242431267900912 2403 50-51 0.0835583872908714 2403 52-53 0.06498873168646213 2403 54-55 -0.04402672026359511 2403 56-57 0.004760204115370925 2403 58-59 -0.018200657715702562 2403 60-61 -0.012152105939691182 2403 62-63 0.041371505605503955 2403 64-65 0.02532422531549372 2403 66-67 0.11085585050964397 2403 68-69 0.12673531516008296 2403 70-71 0.15606111889927377 2403 72-73 0.20754295176421067 2403 74-75 0.10365648396882676 2403 76-77 0.22073082728303461 2403 78-79 0.11875263138756509 2403 80-81 0.14171517086496976 2403 82-83 0.1922856119772689 2403 84-85 0.24520039996686194 2403 86-87 0.32669267767622046 2403 88-89 0.2469559314542522 2403 90-91 0.18617544260046515 2404 1 -0.3901479040136522 2404 2 -0.21246079419655928 2404 3 -0.31372003481574495 2404 4 -0.38127094349090385 2404 5 -0.17580668020951862 2404 6 -0.035236304844460165 2404 7 -0.14758420762948532 2404 8 0.07625221780791236 2404 9 -0.06055794879002718 2404 10-11 0.08105454024181569 2404 12-13 0.1372373882229141 2404 14-15 0.12275367666632775 2404 16-17 0.0571735749960709 2404 18-19 0.10593145516189395 2404 20-21 0.010769699796625787 2404 22-23 0.021598926365982152 2404 24-25 -0.02002235541593933 2404 26-27 0.0651110553701102 2404 28-29 -0.010567265670395898 2404 30-31 0.0639543329382164 2404 32-33 0.05597602602261276 2404 34-35 0.013267127736696693 2404 36-37 -0.0034128644168021083 2404 38-39 -0.019476909692066613 2404 40-41 0.12272293117219135 2404 42-43 0.03783207279244749 2404 44-45 0.09176552736580135 2404 46-47 0.06479814542348805 2404 48-49 0.05658616811018646 2404 50-51 0.05873442861017253 2404 52-53 0.07687844129691257 2404 54-55 0.08292115108344689 2404 56-57 0.12216937096086156 2404 58-59 0.03448014754199136 2404 60-61 0.03807763698098654 2404 62-63 0.11691732258021403 2404 64-65 0.15428845403764058 2404 66-67 0.11406772079682526 2404 68-69 0.14018811209138704 2404 70-71 0.1310766462589612 2404 72-73 0.12142552475675217 2404 74-75 0.1480703619239776 2404 76-77 0.29965664150493865 2404 78-79 0.15995261565477392 2404 80-81 0.01036416146351371 2404 82-83 0.23895141337785475 2404 84-85 0.21489618049433545 2404 86-87 0.07278061815331682 2404 88-89 0.3377569327058154 2404 90-91 -0.14584036767622166 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 13.0 31 347.0 32 2717.0 33 5062.0 34 6454.0 35 8177.0 36 11813.0 37 17966.0 38 46148.0 39 294794.0 40 291364.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.464236537504 13.86572475312004 26.720566783529755 31.94947192584621 2 25.714953271028033 19.03533878504673 27.398510514018692 27.851197429906545 3 25.679787864717028 19.674204127643293 22.006553298113737 32.639454709525936 4 26.293156945630823 21.496229128793686 20.3464967036818 31.864117221893686 5 26.758510925670397 21.525578407108075 20.478641464251556 31.23726920296997 6 26.67586569419804 24.327047331186893 23.492710135722163 25.504376838892906 7 24.780573990114696 24.107584817224083 23.668805805608486 27.443035387052735 8 23.846799687525095 25.325652875426186 24.30762716195399 26.519920275094726 9 23.687057844361213 25.415306889779586 24.861759058486832 26.035876207372365 10-11 23.621715545626447 24.42487825890152 24.608201736133925 27.345204459338106 12-13 23.973249812004003 24.128319133247185 24.401223616677985 27.49720743807083 14-15 24.479123318074627 23.534324784078382 24.274846500354087 27.711705397492896 16-17 24.422834030561212 23.412693197830198 23.999386731497907 28.165086040110683 18-19 24.54935716319513 23.748457702725396 23.87337465594907 27.828810478130407 20-21 24.667265333537756 23.908126537734265 23.625365953376992 27.799242175350987 22-23 24.559286272276612 23.548415357995488 23.805404063633908 28.08689430609399 24-25 24.69442436720182 23.83818472523381 23.74028078936417 27.727110118200205 26-27 24.90213256820787 23.806937234889137 23.646246285710113 27.644683911192878 28-29 24.789992042111102 23.55228479021107 23.787517065656232 27.870206102021594 30-31 24.979794948846724 23.72346786575523 23.75449639959495 27.542240785803095 32-33 25.074239531391925 23.779635568242906 23.667970415407716 27.478154484957457 34-35 24.99048880336617 23.51327277048818 23.75855153397946 27.73768689216619 36-37 25.02983868764417 23.793670061004406 23.859943562142142 27.316547689209287 38-39 25.18785002923454 23.794981323720567 23.70470055940249 27.312468087642404 40-41 25.137458678961604 23.494128699703474 23.849393679987852 27.519018941347067 42-43 25.29604517516929 23.815971284377934 23.75213756123912 27.135845979213656 44-45 25.383888924202935 23.830716562389327 23.699318035287813 27.08607647811992 46-47 25.343152261090403 23.516312904890103 23.7852462408937 27.3552885931258 48-49 25.483815153338067 23.76392999342104 23.769865141367177 26.982389711873715 50-51 25.565672333601498 23.710235112974633 23.729414961347278 26.994677592076595 52-53 25.534821862219538 23.522175872759377 23.767174896848488 27.175827368172595 54-55 25.72106659220997 23.77823234131922 23.633284572288755 26.867416494182063 56-57 25.830935880415133 23.805990198047432 23.508123518165434 26.854950403371998 58-59 25.82445344904511 23.502289229712556 23.54507593937236 27.128181381869975 60-61 25.81451069801677 23.731409609355552 23.569690963149835 26.88438872947784 62-63 26.205555694628718 23.651171135087324 23.291361053395747 26.85191211688821 64-65 26.013009072599136 23.416697544565064 23.43614144890105 27.134151933934746 66-67 26.32679053498193 23.712718135597008 23.153909123963786 26.806582205457275 68-69 26.463931562742836 23.68208211655267 23.036857975926434 26.81712834477806 70-71 26.3276801582991 23.396290884797118 23.2461005331885 27.029928423715287 72-73 26.62146636958641 23.547091862786058 22.93262312584134 26.898818641786193 74-75 26.806337898995153 23.55167802245812 22.683902123416274 26.958081955130446 76-77 26.85471301360013 23.56526234369959 22.58888279947294 26.99114184322734 78-79 27.110040516469624 23.374034388238226 22.38834897693607 27.127576118356085 80-81 27.552911015454885 23.331784672738163 21.944147462342357 27.171156849464595 82-83 28.1911042060641 22.81875891934452 21.490787151925876 27.499349722665507 84-85 28.76315740792127 22.309784116765634 20.862238008028562 28.064820467284534 86-87 29.43534652168217 21.418839250622298 20.094327263199265 29.051486964496263 88-89 29.523789121912557 20.820033846747073 19.813200231357513 29.84297679998286 90-91 27.569515962924818 22.281153450051495 21.513903192584962 28.63542739443872 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 1.5 13 3.0 14 5.5 15 10.0 16 16.0 17 27.0 18 42.5 19 57.0 20 77.0 21 114.0 22 166.0 23 243.0 24 353.5 25 479.0 26 653.5 27 935.5 28 1286.5 29 1692.0 30 2250.5 31 3053.5 32 4017.5 33 4988.5 34 6352.0 35 8083.0 36 10109.0 37 12220.5 38 14599.5 39 17733.0 40 21007.0 41 24436.5 42 28037.0 43 31608.5 44 35333.0 45 38961.0 46 42194.5 47 45009.5 48 47173.5 49 48713.5 50 49747.0 51 50369.5 52 50515.5 53 50012.5 54 48909.5 55 47059.5 56 44938.0 57 43027.5 58 40974.0 59 38578.0 60 36141.5 61 33933.0 62 31997.0 63 29724.5 64 27222.0 65 25089.5 66 22641.0 67 20141.5 68 17955.5 69 15591.0 70 13169.5 71 11018.0 72 9128.0 73 7302.0 74 5616.5 75 4294.0 76 3281.5 77 2406.5 78 1773.5 79 1320.0 80 928.5 81 633.5 82 420.5 83 268.0 84 174.0 85 125.0 86 86.5 87 57.5 88 39.5 89 22.0 90 10.5 91 5.5 92 2.0 93 0.5 94 1.5 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0011681304801746356 2 0.008030897051200619 3 0.0013141467901964649 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 1.6688751447749186E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 3.886846135308887E-4 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 6746.0 32-33 15474.0 34-35 17417.0 36-37 19164.0 38-39 21128.0 40-41 23060.0 42-43 24842.0 44-45 25993.0 46-47 27073.0 48-49 27642.0 50-51 28556.0 52-53 28697.0 54-55 28616.0 56-57 28216.0 58-59 27889.0 60-61 27883.0 62-63 27274.0 64-65 26554.0 66-67 25636.0 68-69 24893.0 70-71 24024.0 72-73 22710.0 74-75 21587.0 76-77 20469.0 78-79 19442.0 80-81 18286.0 82-83 17317.0 84-85 16332.0 86-87 15024.0 88-89 13807.0 90-91 13104.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.71806794532043 #Duplication Level Percentage of deduplicated Percentage of total 1 99.73723752037053 99.4560462773487 2 0.24601815435732768 0.4906491006397266 3 0.01474691073062189 0.04411600338659186 4 0.001250831102915107 0.00498921843634435 5 2.6822751414075705E-4 0.0013373564739946207 6 4.783559244603102E-4 0.0028620437146427867 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0016061794102401239 0.0 0.0 0.0 0.0 2 0.0016061794102401239 0.0 0.0 0.0 1.4601631002182945E-4 3 0.0016061794102401239 0.0 0.0 0.0 1.4601631002182945E-4 4 0.0016061794102401239 0.0 0.0 0.0 2.920326200436589E-4 5 0.0016061794102401239 0.0 0.0 0.0 2.920326200436589E-4 6 0.0016061794102401239 0.0 0.0 0.0 2.920326200436589E-4 7 0.002044228340305612 0.0 0.0 0.0 2.920326200436589E-4 8 0.008468945981266107 0.0 0.0 0.0 2.920326200436589E-4 9 0.008468945981266107 0.0 0.0 0.0 2.920326200436589E-4 10-11 0.008468945981266107 7.300815501091472E-5 0.0 0.0 2.920326200436589E-4 12-13 0.008468945981266107 1.4601631002182945E-4 0.0 0.0 2.920326200436589E-4 14-15 0.008468945981266107 1.4601631002182945E-4 0.0 0.0 2.920326200436589E-4 16-17 0.008468945981266107 1.4601631002182945E-4 0.0 0.0 3.650407750545736E-4 18-19 0.008614962291287937 2.1902446503274417E-4 0.0 0.0 4.380489300654883E-4 20-21 0.008614962291287937 2.920326200436589E-4 0.0 0.0 4.380489300654883E-4 22-23 0.008687970446298852 2.920326200436589E-4 0.0 0.0 4.380489300654883E-4 24-25 0.008906994911331595 2.920326200436589E-4 0.0 0.0 5.840652400873178E-4 26-27 0.009053011221353425 2.920326200436589E-4 0.0 0.0 5.840652400873178E-4 28-29 0.00912601937636434 3.650407750545736E-4 0.0 0.0 7.300815501091472E-4 30-31 0.00927203568638617 4.380489300654883E-4 0.0 0.0 7.300815501091472E-4 32-33 0.009637076461440743 4.380489300654883E-4 0.0 0.0 7.300815501091472E-4 34-35 0.009637076461440743 4.380489300654883E-4 0.0 0.0 7.300815501091472E-4 36-37 0.009783092771462573 4.380489300654883E-4 0.0 0.0 7.300815501091472E-4 38-39 0.009929109081484401 4.380489300654883E-4 0.0 0.0 7.300815501091472E-4 40-41 0.009929109081484401 4.380489300654883E-4 0.0 0.0 8.760978601309766E-4 42-43 0.010002117236495316 5.840652400873178E-4 0.0 0.0 8.760978601309766E-4 44-45 0.010075125391506231 5.840652400873178E-4 0.0 0.0 0.001022114170152806 46-47 0.010221141701528061 5.840652400873178E-4 0.0 0.0 0.0011681304801746356 48-49 0.010221141701528061 5.840652400873178E-4 0.0 0.0 0.0012411386351855501 50-51 0.010440166166560804 5.840652400873178E-4 0.0 0.0 0.0013871549452073796 52-53 0.010513174321571719 5.840652400873178E-4 0.0 0.0 0.0014601631002182944 54-55 0.010513174321571719 5.840652400873178E-4 0.0 0.0 0.0014601631002182944 56-57 0.010513174321571719 5.840652400873178E-4 0.0 0.0 0.0014601631002182944 58-59 0.010513174321571719 5.840652400873178E-4 0.0 0.0 0.0014601631002182944 60-61 0.010659190631593549 5.840652400873178E-4 0.0 0.0 0.0014601631002182944 62-63 0.010805206941615379 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