##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-2B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 685988 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.27380945439279 40.0 40.0 40.0 40.0 40.0 2 39.15104637398905 40.0 40.0 40.0 40.0 40.0 3 39.129305468900334 40.0 40.0 40.0 40.0 40.0 4 39.04766847233479 40.0 40.0 40.0 40.0 40.0 5 39.175046793821465 40.0 40.0 40.0 40.0 40.0 6 39.24945625871006 40.0 40.0 40.0 40.0 40.0 7 39.290967188930416 40.0 40.0 40.0 40.0 40.0 8 39.28672804772095 40.0 40.0 40.0 40.0 40.0 9 39.31567899146924 40.0 40.0 40.0 40.0 40.0 10-11 39.30944564627953 40.0 40.0 40.0 40.0 40.0 12-13 39.31428246558249 40.0 40.0 40.0 40.0 40.0 14-15 39.323451722187556 40.0 40.0 40.0 40.0 40.0 16-17 39.46464952739698 40.0 40.0 40.0 40.0 40.0 18-19 39.475702198872284 40.0 40.0 40.0 40.0 40.0 20-21 39.489320512895276 40.0 40.0 40.0 40.0 40.0 22-23 39.48782777541298 40.0 40.0 40.0 40.0 40.0 24-25 39.48979573986717 40.0 40.0 40.0 40.0 40.0 26-27 39.480684793319995 40.0 40.0 40.0 40.0 40.0 28-29 39.47786841752334 40.0 40.0 40.0 40.0 40.0 30-31 39.47106907501795 40.0 40.0 40.0 40.0 40.0 32-33 39.47088248927068 40.0 40.0 40.0 40.0 40.0 34-35 39.47137467778266 40.0 40.0 40.0 40.0 40.0 36-37 39.466517364558364 40.0 40.0 40.0 40.0 40.0 38-39 39.45173763265129 40.0 40.0 40.0 40.0 40.0 40-41 39.43779664386659 40.0 40.0 40.0 40.0 40.0 42-43 39.43424107452206 40.0 40.0 40.0 40.0 40.0 44-45 39.41616701442737 40.0 40.0 40.0 40.0 40.0 46-47 39.41159999322171 40.0 40.0 40.0 40.0 40.0 48-49 39.41313658091788 40.0 40.0 40.0 40.0 40.0 50-51 39.41076657285969 40.0 40.0 40.0 40.0 40.0 52-53 39.40266393451517 40.0 40.0 40.0 40.0 40.0 54-55 39.37281937840602 40.0 40.0 40.0 40.0 40.0 56-57 39.37843859006944 40.0 40.0 40.0 40.0 40.0 58-59 39.36453742361929 40.0 40.0 40.0 40.0 40.0 60-61 39.33232808069662 40.0 40.0 40.0 40.0 40.0 62-63 39.37940784469603 40.0 40.0 40.0 40.0 40.0 64-65 39.37848632405917 40.0 40.0 40.0 40.0 40.0 66-67 39.365049539992114 40.0 40.0 40.0 40.0 40.0 68-69 39.367760278530014 40.0 40.0 40.0 40.0 40.0 70-71 39.36297711586204 40.0 40.0 40.0 40.0 40.0 72-73 39.35803709962826 40.0 40.0 40.0 40.0 40.0 74-75 39.34467556338828 40.0 40.0 40.0 40.0 40.0 76-77 39.3462507470382 40.0 40.0 40.0 40.0 40.0 78-79 39.32627981512975 40.0 40.0 40.0 40.0 40.0 80-81 39.323073963312666 40.0 40.0 40.0 40.0 40.0 82-83 39.29868983084191 40.0 40.0 40.0 40.0 40.0 84-85 39.3222050019218 40.0 40.0 40.0 40.0 40.0 86-87 39.307348748916766 40.0 40.0 40.0 40.0 40.0 88-89 39.36285429014052 40.0 40.0 40.0 40.0 40.0 90-91 39.438074524270775 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18999849719575934 1101 2 0.1159788931038861 1101 3 0.32672105530109263 1101 4 0.19048925713898512 1101 5 0.1879247653626237 1101 6 0.133430954453452 1101 7 -0.030663406104793012 1101 8 0.22331270189967256 1101 9 0.057375473377454966 1101 10-11 0.08360361637235769 1101 12-13 0.07338139480129513 1101 14-15 0.04057738865634519 1101 16-17 0.07227824926793858 1101 18-19 0.11752289864607235 1101 20-21 0.15940725768052033 1101 22-23 0.1310644358151407 1101 24-25 0.06728349605152317 1101 26-27 0.1839407863145297 1101 28-29 0.15496389156198376 1101 30-31 0.1123094769020696 1101 32-33 0.07236166497361296 1101 34-35 0.08740082347050304 1101 36-37 0.16772076200048502 1101 38-39 -0.003901483963517194 1101 40-41 0.08226426063332326 1101 42-43 0.036603351931432826 1101 44-45 0.22523639299097908 1101 46-47 0.11808326781691392 1101 48-49 0.018210460510090343 1101 50-51 0.14139398122417646 1101 52-53 0.1905116071894497 1101 54-55 -0.019462556390493546 1101 56-57 0.16099481755501444 1101 58-59 0.02418096578205109 1101 60-61 0.04237626820930984 1101 62-63 0.08920343249097584 1101 64-65 -0.06938142327397401 1101 66-67 0.08093145805356272 1101 68-69 -0.08345954957496104 1101 70-71 0.16045240799380878 1101 72-73 0.07600807194047121 1101 74-75 0.05604861168276898 1101 76-77 0.014435104057746173 1101 78-79 -0.14511962801603318 1101 80-81 0.11995005632523714 1101 82-83 -0.197561216003713 1101 84-85 -0.1587249872390899 1101 86-87 -0.8363736720850596 1101 88-89 0.15386055868491155 1101 90-91 0.6071649253183153 1102 1 0.30527191719710345 1102 2 0.24424607562090728 1102 3 0.08491896412031252 1102 4 0.19007716556266985 1102 5 0.02257302036636588 1102 6 -0.03415417971086754 1102 7 0.11740992779884607 1102 8 0.14559296648454279 1102 9 0.0698743580626413 1102 10-11 0.01360139954044115 1102 12-13 -0.1405530035312097 1102 14-15 -0.11816617356258519 1102 16-17 0.10857910187437625 1102 18-19 0.025409393172523664 1102 20-21 -7.326326920775728E-4 1102 22-23 0.0625615700309794 1102 24-25 -0.04289397008200524 1102 26-27 0.029218796353745802 1102 28-29 -0.022178455722674073 1102 30-31 0.0246995436553874 1102 32-33 0.15772257473130935 1102 34-35 0.11317793851959834 1102 36-37 0.025336175092533608 1102 38-39 0.053342100328137576 1102 40-41 0.060796029176017896 1102 42-43 0.05218013416654799 1102 44-45 0.09922246219315412 1102 46-47 -0.042039496212083804 1102 48-49 0.013729575867643007 1102 50-51 -0.05217712634183158 1102 52-53 -0.1351641674072681 1102 54-55 0.05833098704355422 1102 56-57 -0.0030821588911322806 1102 58-59 0.045032459408808734 1102 60-61 0.007145360908999976 1102 62-63 0.011069313109246082 1102 64-65 0.04270507238992849 1102 66-67 -0.06325662230248241 1102 68-69 -0.009902284293453079 1102 70-71 0.04330329179064307 1102 72-73 0.18715175451427513 1102 74-75 0.09428093597207265 1102 76-77 0.00883740782546738 1102 78-79 0.12122949400723826 1102 80-81 0.05221995163392279 1102 82-83 -0.07307217847119318 1102 84-85 0.1251584312880496 1102 86-87 0.0022062542675058694 1102 88-89 0.12460063922891607 1102 90-91 0.13657669002419937 1103 1 0.2971597265496655 1103 2 0.09385596699287646 1103 3 0.2088909073313232 1103 4 0.03029524305502207 1103 5 0.048765441504180274 1103 6 -0.07001162006889672 1103 7 -0.09155017296463797 1103 8 0.014312019818909505 1103 9 -0.07295422992514489 1103 10-11 -0.1564820071901991 1103 12-13 -0.10246428481252678 1103 14-15 -0.009930614579282349 1103 16-17 0.010550713466429329 1103 18-19 0.007673559614815417 1103 20-21 0.018047952691432556 1103 22-23 -0.017825906942043446 1103 24-25 0.01201361014924629 1103 26-27 0.011521905205384542 1103 28-29 -0.018259875781971857 1103 30-31 0.05902180121987044 1103 32-33 -0.017077236104654503 1103 34-35 0.054447091264520964 1103 36-37 0.005148284817792614 1103 38-39 0.024966770543599637 1103 40-41 -0.009126211709087784 1103 42-43 -0.09066545605326581 1103 44-45 -0.0014435346773780111 1103 46-47 0.026993330784030434 1103 48-49 0.056050468941649 1103 50-51 -0.11773952095946072 1103 52-53 0.047881851295748845 1103 54-55 0.0719790064980046 1103 56-57 0.05092005127075083 1103 58-59 0.01647746401443584 1103 60-61 0.011082993137783603 1103 62-63 0.09024121285963105 1103 64-65 -0.15651413650130053 1103 66-67 -0.0635391158451597 1103 68-69 0.06414444213287851 1103 70-71 -0.027384460018524237 1103 72-73 0.03501030544054373 1103 74-75 -0.12618492676560322 1103 76-77 -0.026708137857752945 1103 78-79 0.13000287543390243 1103 80-81 0.04014182303716041 1103 82-83 -0.2761455114266198 1103 84-85 -0.30247834895151726 1103 86-87 -0.11925251417225269 1103 88-89 -0.493180109434185 1103 90-91 -0.16672784857445322 1104 1 0.4337974085678269 1104 2 0.2565205795515766 1104 3 0.06430738265981262 1104 4 0.15905818615721756 1104 5 0.08405067118406606 1104 6 0.0893555566683304 1104 7 0.1452667497248612 1104 8 0.07043064455412207 1104 9 -0.061660586302998865 1104 10-11 0.10755283364111534 1104 12-13 0.01804469944094933 1104 14-15 0.07685742221690361 1104 16-17 0.034385278770770356 1104 18-19 0.02142028217691916 1104 20-21 -0.01848159239278857 1104 22-23 0.02285135615660039 1104 24-25 0.049743030801913335 1104 26-27 -0.008040510544091717 1104 28-29 0.05982091583963722 1104 30-31 0.04322788355207763 1104 32-33 0.07056958669434721 1104 34-35 0.024541508983602967 1104 36-37 0.04739812087240125 1104 38-39 0.022578195303864845 1104 40-41 0.04403039926721419 1104 42-43 -0.043938107366415124 1104 44-45 0.06580719778971655 1104 46-47 -4.873153537232611E-5 1104 48-49 0.07020149113202478 1104 50-51 -0.05245907112388437 1104 52-53 0.021231967137289587 1104 54-55 0.06795764304123253 1104 56-57 0.08649281417621779 1104 58-59 -0.017677746288995877 1104 60-61 -0.035925193489298124 1104 62-63 0.060380574869903114 1104 64-65 -0.03599346736639575 1104 66-67 0.0818183962000063 1104 68-69 0.020172769272143398 1104 70-71 0.014874003294558236 1104 72-73 -0.1341300042289859 1104 74-75 0.10646513925669865 1104 76-77 -0.015160843957517045 1104 78-79 0.00840579393044294 1104 80-81 -0.14498939732635563 1104 82-83 0.04528860321813255 1104 84-85 0.2280741469378853 1104 86-87 0.22982614117358224 1104 88-89 -0.11823534279513126 1104 90-91 -1.0678922829196722 1201 1 0.3262757445889548 1201 2 0.34135651815992674 1201 3 0.2017975748778582 1201 4 0.32660144897102583 1201 5 0.09223969862848236 1201 6 0.1264708093254825 1201 7 0.08866390481665576 1201 8 -0.0479298127852843 1201 9 0.11098648699695701 1201 10-11 0.014617042402825575 1201 12-13 0.05085524059889224 1201 14-15 -0.017174524370858535 1201 16-17 -0.010923054731456716 1201 18-19 -0.03142795560714973 1201 20-21 0.04321116562788063 1201 22-23 0.04498523998034898 1201 24-25 0.001745555491474704 1201 26-27 0.04595252339734657 1201 28-29 0.09179356117576987 1201 30-31 0.02744915290344352 1201 32-33 0.007288004672410864 1201 34-35 -0.0010752972897520863 1201 36-37 -0.004217472116408771 1201 38-39 -0.0493111433465927 1201 40-41 0.04204599571218637 1201 42-43 0.08726641157682025 1201 44-45 0.08286822946416805 1201 46-47 -0.016346058904062488 1201 48-49 -0.05341197062584513 1201 50-51 0.021876497021281693 1201 52-53 -0.008657815974615346 1201 54-55 0.09382825980618748 1201 56-57 0.08926768474930213 1201 58-59 0.037597019518841535 1201 60-61 0.030844578519612753 1201 62-63 0.03598847436477115 1201 64-65 0.010394112290235569 1201 66-67 -0.14200711992090476 1201 68-69 0.2782271233948066 1201 70-71 -0.13036213480842918 1201 72-73 -0.08063205334406831 1201 74-75 -0.1597063884006502 1201 76-77 0.18042852996492798 1201 78-79 -0.056029901763579915 1201 80-81 -0.34610248189331116 1201 82-83 -0.1313278160139646 1201 84-85 0.15705240691681865 1201 86-87 0.20872211281321995 1201 88-89 0.2542489448663332 1201 90-91 0.0738315919849839 1202 1 0.0031870453002653676 1202 2 0.15820712829695083 1202 3 -0.07586481836431602 1202 4 -0.14221700923562963 1202 5 -0.09260431682994863 1202 6 0.07984620192571157 1202 7 -0.022276052300696847 1202 8 -0.0030285534486864663 1202 9 -0.08968935266847922 1202 10-11 -0.0702170638042432 1202 12-13 -0.02861695533429298 1202 14-15 0.02960001431040382 1202 16-17 -0.05387980300343287 1202 18-19 -0.09233452285577926 1202 20-21 0.03131202170104075 1202 22-23 0.03943031472338987 1202 24-25 -0.04524555059639823 1202 26-27 0.039707684465767557 1202 28-29 -0.021272433002188507 1202 30-31 -0.05371249858099958 1202 32-33 -0.02662907455882646 1202 34-35 -0.06212050305548189 1202 36-37 -0.023895551965900097 1202 38-39 -0.024066166883883966 1202 40-41 -0.04643133512651332 1202 42-43 -0.0853307541859678 1202 44-45 6.921324162192377E-4 1202 46-47 -0.05976337694731626 1202 48-49 -0.04081811624961773 1202 50-51 0.08838654484092956 1202 52-53 -0.0014473218657755638 1202 54-55 0.05713164056219 1202 56-57 -0.01084599609919934 1202 58-59 -0.07835453264555525 1202 60-61 -0.03548166725067148 1202 62-63 -0.09356558609474774 1202 64-65 0.0058779149879839565 1202 66-67 -0.002837649616552085 1202 68-69 -0.13157627471677102 1202 70-71 -0.14878535034276297 1202 72-73 -0.34368103658313487 1202 74-75 0.014017800372116085 1202 76-77 -0.16985804351860878 1202 78-79 0.11711983441124829 1202 80-81 0.13299651688714675 1202 82-83 -0.0157177941553428 1202 84-85 -0.5021104890107821 1202 86-87 0.42328496868377385 1202 88-89 0.0851254335757119 1202 90-91 -0.10712078896739996 1203 1 0.23888749363147355 1203 2 0.2294587231440488 1203 3 0.23724209280536002 1203 4 0.2014303546364573 1203 5 0.16926539037102373 1203 6 0.22596783181332114 1203 7 0.11929272375083855 1203 8 0.11931491736437039 1203 9 -0.027815955498688538 1203 10-11 0.03125878180178887 1203 12-13 0.06156020574562149 1203 14-15 -0.03046983963778871 1203 16-17 0.12186092275666027 1203 18-19 0.1046172790553399 1203 20-21 0.05079392169436403 1203 22-23 0.016172157408668397 1203 24-25 0.11584088976164253 1203 26-27 -0.0029756040912189974 1203 28-29 -0.042092372458320426 1203 30-31 0.11787502535057115 1203 32-33 0.0625170545646796 1203 34-35 0.01760175970973421 1203 36-37 0.09392492208193204 1203 38-39 0.06494053694845547 1203 40-41 0.053581586712383 1203 42-43 0.05525413374559207 1203 44-45 0.046815804038914166 1203 46-47 0.1339076869235214 1203 48-49 0.005969176122953002 1203 50-51 0.08547283887526191 1203 52-53 0.00892701452880118 1203 54-55 0.010862072111606835 1203 56-57 -0.041887745412459765 1203 58-59 0.054979669297694045 1203 60-61 0.046563526565179814 1203 62-63 -0.033710402511914594 1203 64-65 0.07938891655947344 1203 66-67 -0.07269070938699684 1203 68-69 0.10031025611390731 1203 70-71 0.09925703598721469 1203 72-73 0.037860106848746966 1203 74-75 -0.25071522182654604 1203 76-77 0.02389997425633794 1203 78-79 -0.26871590993250294 1203 80-81 -0.4244569436572476 1203 82-83 -0.046785852094707536 1203 84-85 -0.26530171226050214 1203 86-87 -0.050464342394853645 1203 88-89 0.026903676010931576 1203 90-91 -0.12549500734161967 1204 1 0.26664691200556945 1204 2 0.19857558337128722 1204 3 -0.033018820548747385 1204 4 -0.12696167633323796 1204 5 -0.21587293462189194 1204 6 -0.02470097503525892 1204 7 -0.13700979725756923 1204 8 -0.04007355712655425 1204 9 -0.20172934107763751 1204 10-11 -0.009998732439861158 1204 12-13 -0.10865507137646091 1204 14-15 -0.016515381450020072 1204 16-17 3.898624224092373E-4 1204 18-19 -0.13238032897663743 1204 20-21 -0.014375404463066843 1204 22-23 -0.08406231840412914 1204 24-25 -0.09849990273513498 1204 26-27 -0.06468680542567995 1204 28-29 0.06726934975866072 1204 30-31 -0.11198602019123882 1204 32-33 -0.07246965453944654 1204 34-35 -0.03772769467641979 1204 36-37 -0.011677971195780401 1204 38-39 -0.1370812409860207 1204 40-41 -0.05942041904211948 1204 42-43 -0.1355149528021542 1204 44-45 -0.02004899039423691 1204 46-47 0.012842638204134005 1204 48-49 -0.04316366722285636 1204 50-51 0.038804124120474626 1204 52-53 -0.07665160234143542 1204 54-55 -0.20265616486273075 1204 56-57 -0.13811911938180543 1204 58-59 -0.12143690030502796 1204 60-61 -0.05233543094735893 1204 62-63 -0.055224263227742654 1204 64-65 -0.17710741258999008 1204 66-67 -0.02226689003241944 1204 68-69 -0.15230235736847675 1204 70-71 -0.19285917253766627 1204 72-73 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0.1514043940154295 2401 52-53 0.04756053301724705 2401 54-55 0.18180615428279623 2401 56-57 0.17550240706150788 2401 58-59 0.11644219750901641 2401 60-61 0.1831053880494906 2401 62-63 0.08189232986568129 2401 64-65 0.0477230132426385 2401 66-67 0.09896511466433822 2401 68-69 0.2175959804079426 2401 70-71 0.1942880532003315 2401 72-73 0.1508415006260435 2401 74-75 0.2951204570992729 2401 76-77 0.1778580086759618 2401 78-79 0.0644545096489395 2401 80-81 0.17084578152628183 2401 82-83 0.49484962985541614 2401 84-85 0.17370111279219458 2401 86-87 0.19000047349533133 2401 88-89 -0.20584979269210635 2401 90-91 0.16272048087387248 2402 1 0.3353012230450503 2402 2 0.2793572110908684 2402 3 0.25755329870991517 2402 4 0.2609743199830987 2402 5 0.13985401369168216 2402 6 0.13009354551268615 2402 7 0.07702266504242061 2402 8 0.20944296180979194 2402 9 -0.023342095768555282 2402 10-11 0.07412985050016374 2402 12-13 0.12787652756448153 2402 14-15 0.13765464272402994 2402 16-17 -0.00475575746937551 2402 18-19 -0.056154359136641574 2402 20-21 0.0139974515286454 2402 22-23 -0.02366747553548265 2402 24-25 0.0071694883089534756 2402 26-27 -0.0011608470971467 2402 28-29 0.008163789726431503 2402 30-31 0.07997035139849373 2402 32-33 0.04694167675022243 2402 34-35 -0.02957953617487874 2402 36-37 0.05187349960817755 2402 38-39 0.029016467162165327 2402 40-41 0.054257064194644045 2402 42-43 0.015944668009737484 2402 44-45 0.05715139263526936 2402 46-47 0.07700153261063747 2402 48-49 0.11960578617639328 2402 50-51 0.012504271953034163 2402 52-53 0.04776849368909808 2402 54-55 0.027723315530039372 2402 56-57 0.08476441661348133 2402 58-59 0.04751214026657635 2402 60-61 0.08895580380319501 2402 62-63 0.08935002654517632 2402 64-65 0.07393294601374123 2402 66-67 -0.008296991858912861 2402 68-69 0.009212342582479494 2402 70-71 0.09253369796220312 2402 72-73 -0.02459868905773277 2402 74-75 0.028532030775139106 2402 76-77 -0.16333924099939878 2402 78-79 -0.03180319366619244 2402 80-81 0.12428112983492667 2402 82-83 0.019654948868129907 2402 84-85 -0.02272845429664727 2402 86-87 0.2631646412639981 2402 88-89 0.30030728552380026 2402 90-91 -0.4166250664782396 2403 1 -0.2141366254278836 2403 2 -0.3088266846952976 2403 3 -0.04212084473024191 2403 4 -0.10765788091206474 2403 5 -0.0028907440551364516 2403 6 0.10512416518248813 2403 7 0.08871975553352485 2403 8 0.0021065943797751174 2403 9 0.11459511018092883 2403 10-11 0.15764072519076677 2403 12-13 0.21797142217707233 2403 14-15 0.13109412424602596 2403 16-17 0.05587857060776713 2403 18-19 0.05279200785648186 2403 20-21 0.03445093900734264 2403 22-23 0.05669721866704691 2403 24-25 0.008610608906010953 2403 26-27 0.020645479913397935 2403 28-29 0.06462510499378027 2403 30-31 -0.037330176329447795 2403 32-33 0.016742794072825973 2403 34-35 0.029697523718027696 2403 36-37 0.0956143927190709 2403 38-39 0.14603013755389327 2403 40-41 -1.6248721982492498E-4 2403 42-43 0.047847589202959284 2403 44-45 0.0898010983060118 2403 46-47 0.15011542253036225 2403 48-49 0.10922843624804557 2403 50-51 0.016765175330206716 2403 52-53 -0.034753455544773715 2403 54-55 -0.08650634822660663 2403 56-57 -0.034705968568601975 2403 58-59 0.014347008584593368 2403 60-61 0.009183496956119086 2403 62-63 -0.03157973284055515 2403 64-65 0.062257351444152675 2403 66-67 -0.013391510764321879 2403 68-69 0.007481250597614064 2403 70-71 0.01800923401985699 2403 72-73 0.05377633623452738 2403 74-75 0.11332293266335114 2403 76-77 0.13516402645343817 2403 78-79 -3.914988953539478E-4 2403 80-81 0.06847512566886849 2403 82-83 0.30718625874376215 2403 84-85 0.153267163345582 2403 86-87 0.36352446898955293 2403 88-89 0.09603180029118619 2403 90-91 -0.05276773461434914 2404 1 -0.4471884511020434 2404 2 -0.14244486129460654 2404 3 -0.30459974482267427 2404 4 -0.15197053962769758 2404 5 -0.24649362075267334 2404 6 -0.13902469768281378 2404 7 -0.13938132739755815 2404 8 0.102151976188253 2404 9 0.0502652994239412 2404 10-11 0.12340862137966013 2404 12-13 0.09387280408386545 2404 14-15 0.07781921669938896 2404 16-17 0.010289603390639002 2404 18-19 0.03212441248665243 2404 20-21 -6.888009733216904E-4 2404 22-23 -0.0015178981274672765 2404 24-25 -0.0017885842282581166 2404 26-27 0.028499956166065488 2404 28-29 0.022643859881974038 2404 30-31 -0.029482722406896755 2404 32-33 0.018897425809697665 2404 34-35 -0.024009901837686698 2404 36-37 0.05892500220421937 2404 38-39 0.007773151067596018 2404 40-41 0.031862376725811714 2404 42-43 0.02481634356783502 2404 44-45 0.06802112799972804 2404 46-47 0.09736885870496792 2404 48-49 -0.017267083128402305 2404 50-51 0.02917327244318102 2404 52-53 -0.07225892060964867 2404 54-55 -0.019588280839855088 2404 56-57 -0.04165595929223542 2404 58-59 0.033465175783092604 2404 60-61 -0.10358285045052895 2404 62-63 0.0888479281010035 2404 64-65 0.04443382696923237 2404 66-67 0.12625193737178364 2404 68-69 0.10442876942469326 2404 70-71 0.22492018972888417 2404 72-73 0.18733081068965873 2404 74-75 -0.006631175553920343 2404 76-77 0.023455621304307783 2404 78-79 0.06458227594305299 2404 80-81 -0.14922751530343703 2404 82-83 0.1275138590713638 2404 84-85 0.16688213657413797 2404 86-87 -0.13302970719914242 2404 88-89 0.27317557262358605 2404 90-91 -0.03553659973614742 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 12.0 31 400.0 32 2763.0 33 4989.0 34 6421.0 35 8231.0 36 11753.0 37 18346.0 38 49288.0 39 312013.0 40 271772.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.740142891088187 13.782677844884011 26.649289990772285 31.827889273255515 2 25.78587652823499 19.26151953137529 27.27132239525777 27.681281545131952 3 25.53831665891621 19.910901322339832 21.914455356635244 32.636326662108715 4 26.232091523466885 21.765395313037548 20.138544697574883 31.86396846592069 5 26.704257217327417 21.752129774864866 20.350647533193 31.192965474614713 6 26.781372268902665 24.85976431074596 23.234371446730847 25.12449197362053 7 24.64503752252226 24.470690449395615 23.68467086887817 27.19960115920395 8 23.62592348554202 25.936167979614805 24.488620792200447 25.949287742642728 9 23.431167892149716 26.06984378735488 24.903351079027622 25.595637241467784 10-11 23.511854434771454 24.844239257829585 24.694601071738866 26.9493052356601 12-13 23.98117751330927 24.473824615007842 24.502615206096902 27.042382665585983 14-15 24.383954238266558 23.980229974868365 24.380309859647692 27.25550592721739 16-17 24.295104287538557 23.8352565934098 24.123089616727988 27.74654950232366 18-19 24.45684181064392 24.05246155909433 23.98394724105961 27.506749389202145 20-21 24.604001819273808 24.177973958728142 23.78911876009493 27.428905461903124 22-23 24.44459669848452 23.917255112334328 23.88846452124527 27.74968366793588 24-25 24.56835979638128 24.221779389726933 23.85799751599153 27.351863297900252 26-27 24.761949188615546 24.19320746135501 23.833653066817494 27.21119028321195 28-29 24.733814585677884 23.774031032612815 23.866816912249195 27.62533746946011 30-31 24.844694691958672 24.095191238907415 23.83592995499189 27.22418411414202 32-33 24.879993491172403 24.148933809661173 23.780149555106842 27.190923144059585 34-35 24.79491330529246 23.849214754743244 23.92622628377765 27.42964565618664 36-37 24.914695636514924 24.09006091616421 23.922013648698158 27.073229798622712 38-39 25.07900621257048 24.1113449963265 23.79340145229128 27.016247338811738 40-41 24.96411847187682 23.89726308270279 23.91214482247738 27.226473622943015 42-43 25.0385454845307 24.120926740817154 23.935994310299954 26.90453346435219 44-45 25.206903223032718 24.15106980610212 23.742189627251754 26.89983734361341 46-47 25.190443862662875 23.84064602759045 23.82498668487026 27.143923424876416 48-49 25.327184160688216 24.121076131502647 23.74230655097862 26.80943315683052 50-51 25.457583020084385 24.144697578062264 23.681724488828756 26.715994913024595 52-53 25.354680745839147 23.91183847601514 23.804518715127994 26.92896206301772 54-55 25.48821215294484 24.16638364512873 23.678407343465505 26.66699685846093 56-57 25.715241950252278 24.074034314437448 23.629095914977995 26.581627820332283 58-59 25.597620518949604 23.93307030470124 23.654785106513014 26.81452406983614 60-61 25.711294240825378 24.148711523643144 23.546236331445176 26.593757904086303 62-63 25.935188829897882 24.066731311424522 23.320276372341002 26.677803486336593 64-65 25.872848021240515 23.867344988259145 23.381050837292257 26.878756153208084 66-67 26.077629602094078 24.035631863451204 23.297205829964533 26.58953270449019 68-69 26.257025328853196 23.926693343722892 23.146830718364765 26.669450609059147 70-71 26.185962836410177 23.8146408606305 23.20031875203748 26.799077550921847 72-73 26.56436604129374 23.980705901643827 22.738340871513877 26.71658718554856 74-75 26.544633164288463 24.007708696373093 22.765369682673267 26.682288456665177 76-77 26.77426293397303 23.695118794591263 22.527041856090296 27.00357641534541 78-79 26.899220788196065 23.91713747645951 22.238635774633288 26.94500596071114 80-81 27.448794833956065 23.81184129276691 21.890273896616325 26.8490899766607 82-83 27.587473115459265 23.439385196453863 21.60140061900016 27.371741069086713 84-85 28.572042160964173 22.890448497135154 20.971385865596893 27.566123476303783 86-87 29.482559902942068 21.89384288747346 20.508340915984228 28.115256293600243 88-89 29.385668244936937 21.091791668324515 19.979707953686386 29.542832133052162 90-91 27.086758874165778 22.92774929877164 21.43824354386304 28.547248283199533 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 3.0 12 4.5 13 3.5 14 6.0 15 11.0 16 17.0 17 25.0 18 40.0 19 61.0 20 88.0 21 130.5 22 188.5 23 272.5 24 390.0 25 555.5 26 777.5 27 1053.5 28 1423.0 29 1912.0 30 2547.5 31 3354.5 32 4325.0 33 5390.0 34 6864.0 35 8799.5 36 10901.0 37 13099.5 38 15825.0 39 19142.5 40 22388.5 41 25859.5 42 29492.0 43 33255.0 44 36889.0 45 39927.5 46 42740.5 47 45462.5 48 47731.0 49 49038.5 50 49819.0 51 50088.5 52 49696.5 53 49057.0 54 47777.0 55 45898.0 56 43613.5 57 41248.5 58 39033.5 59 36750.0 60 34561.0 61 32420.5 62 30362.0 63 28275.0 64 26057.5 65 23664.0 66 21087.5 67 18869.0 68 16837.0 69 14565.0 70 12381.0 71 10326.0 72 8406.5 73 6655.5 74 5063.0 75 3846.5 76 2934.0 77 2166.0 78 1604.5 79 1174.0 80 813.5 81 550.0 82 374.5 83 243.0 84 157.0 85 111.0 86 75.5 87 42.5 88 27.0 89 21.0 90 13.5 91 7.5 92 4.5 93 4.0 94 2.5 95 1.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0016035265923019064 2 0.009621159553811437 3 0.0010204260132830312 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 2.4147823315206365E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 6473.0 32-33 14636.0 34-35 16271.0 36-37 18249.0 38-39 20192.0 40-41 22205.0 42-43 23590.0 44-45 24807.0 46-47 25754.0 48-49 26763.0 50-51 28212.0 52-53 27777.0 54-55 28117.0 56-57 27858.0 58-59 28124.0 60-61 27520.0 62-63 27645.0 64-65 26987.0 66-67 26137.0 68-69 25527.0 70-71 24260.0 72-73 23421.0 74-75 22751.0 76-77 21778.0 78-79 20436.0 80-81 19631.0 82-83 18313.0 84-85 17186.0 86-87 16390.0 88-89 15154.0 90-91 13824.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.7710794622358 #Duplication Level Percentage of deduplicated Percentage of total 1 99.78065305271134 99.55223464515845 2 0.21074762123804922 0.42053035330037164 3 0.007755797035753096 0.02321412727041285 4 6.248677021945871E-4 0.0024937490067616338 5 0.0 0.0 6 0.0 0.0 7 2.1866131265364818E-4 0.0015271252640058745 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.3732543426415623E-4 0.0 0.0 0.0 0.0 2 4.3732543426415623E-4 1.4577514475471874E-4 0.0 0.0 0.0 3 4.3732543426415623E-4 2.915502895094375E-4 0.0 0.0 0.0 4 4.3732543426415623E-4 2.915502895094375E-4 0.0 0.0 0.0 5 4.3732543426415623E-4 2.915502895094375E-4 0.0 0.0 0.0 6 4.3732543426415623E-4 2.915502895094375E-4 0.0 0.0 1.4577514475471874E-4 7 7.288757237735937E-4 2.915502895094375E-4 0.0 0.0 1.4577514475471874E-4 8 0.009621159553811437 2.915502895094375E-4 0.0 0.0 1.4577514475471874E-4 9 0.009766934698566156 2.915502895094375E-4 0.0 0.0 1.4577514475471874E-4 10-11 0.009912709843320874 2.915502895094375E-4 0.0 0.0 1.4577514475471874E-4 12-13 0.009912709843320874 2.915502895094375E-4 7.288757237735937E-5 0.0 1.4577514475471874E-4 14-15 0.009912709843320874 2.915502895094375E-4 1.4577514475471874E-4 0.0 2.1866271713207811E-4 16-17 0.009912709843320874 2.915502895094375E-4 1.4577514475471874E-4 0.0 2.915502895094375E-4 18-19 0.009985597415698233 2.915502895094375E-4 1.4577514475471874E-4 0.0 2.915502895094375E-4 20-21 0.010058484988075593 2.915502895094375E-4 1.4577514475471874E-4 0.0 2.915502895094375E-4 22-23 0.010131372560452953 2.915502895094375E-4 1.4577514475471874E-4 0.0 2.915502895094375E-4 24-25 0.01027714770520767 2.915502895094375E-4 1.4577514475471874E-4 0.0 4.3732543426415623E-4 26-27 0.01042292284996239 2.915502895094375E-4 1.4577514475471874E-4 0.0 4.3732543426415623E-4 28-29 0.010641585567094469 4.3732543426415623E-4 1.4577514475471874E-4 0.0 5.83100579018875E-4 30-31 0.010641585567094469 4.3732543426415623E-4 1.4577514475471874E-4 0.0 7.288757237735937E-4 32-33 0.010933135856603906 4.3732543426415623E-4 2.915502895094375E-4 0.0 7.288757237735937E-4 34-35 0.011078911001358624 4.3732543426415623E-4 2.915502895094375E-4 0.0 7.288757237735937E-4 36-37 0.011224686146113343 4.3732543426415623E-4 2.915502895094375E-4 0.0 7.288757237735937E-4 38-39 0.011370461290868062 4.3732543426415623E-4 2.915502895094375E-4 0.0 7.288757237735937E-4 40-41 0.01158912400800014 4.3732543426415623E-4 2.915502895094375E-4 0.0 8.017632961509531E-4 42-43 0.0116620115803775 4.3732543426415623E-4 2.915502895094375E-4 0.0 8.746508685283125E-4 44-45 0.01173489915275486 5.102130066415156E-4 2.915502895094375E-4 0.0 8.746508685283125E-4 46-47 0.011953561869886936 5.83100579018875E-4 2.915502895094375E-4 0.0 8.746508685283125E-4 48-49 0.012099337014641654 5.83100579018875E-4 2.915502895094375E-4 0.0 8.746508685283125E-4 50-51 0.012317999731773733 5.83100579018875E-4 2.915502895094375E-4 0.0 8.746508685283125E-4 52-53 0.01260955002128317 5.83100579018875E-4 2.915502895094375E-4 0.0 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