##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-2A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 700536 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.252746468418465 40.0 40.0 40.0 40.0 40.0 2 39.14711021275138 40.0 40.0 40.0 40.0 40.0 3 39.13164491189603 40.0 40.0 40.0 40.0 40.0 4 39.05571733643953 40.0 40.0 40.0 40.0 40.0 5 39.189689038107964 40.0 40.0 40.0 40.0 40.0 6 39.24875809380246 40.0 40.0 40.0 40.0 40.0 7 39.2850845638197 40.0 40.0 40.0 40.0 40.0 8 39.29772631242363 40.0 40.0 40.0 40.0 40.0 9 39.334880719905904 40.0 40.0 40.0 40.0 40.0 10-11 39.331774526933664 40.0 40.0 40.0 40.0 40.0 12-13 39.33121495540558 40.0 40.0 40.0 40.0 40.0 14-15 39.34736259093037 40.0 40.0 40.0 40.0 40.0 16-17 39.48050921009056 40.0 40.0 40.0 40.0 40.0 18-19 39.486890038484816 40.0 40.0 40.0 40.0 40.0 20-21 39.50607534801923 40.0 40.0 40.0 40.0 40.0 22-23 39.504470862311145 40.0 40.0 40.0 40.0 40.0 24-25 39.51205648246486 40.0 40.0 40.0 40.0 40.0 26-27 39.5024581177841 40.0 40.0 40.0 40.0 40.0 28-29 39.50077654824305 40.0 40.0 40.0 40.0 40.0 30-31 39.49080382029189 40.0 40.0 40.0 40.0 40.0 32-33 39.4890452964559 40.0 40.0 40.0 40.0 40.0 34-35 39.48884465829026 40.0 40.0 40.0 40.0 40.0 36-37 39.48670913714487 40.0 40.0 40.0 40.0 40.0 38-39 39.47567936611891 40.0 40.0 40.0 40.0 40.0 40-41 39.45937245561812 40.0 40.0 40.0 40.0 40.0 42-43 39.4633105980869 40.0 40.0 40.0 40.0 40.0 44-45 39.43563059854815 40.0 40.0 40.0 40.0 40.0 46-47 39.44054640013428 40.0 40.0 40.0 40.0 40.0 48-49 39.4351807080168 40.0 40.0 40.0 40.0 40.0 50-51 39.43573947486475 40.0 40.0 40.0 40.0 40.0 52-53 39.41914452189893 40.0 40.0 40.0 40.0 40.0 54-55 39.40675643331452 40.0 40.0 40.0 40.0 40.0 56-57 39.406443562614996 40.0 40.0 40.0 40.0 40.0 58-59 39.391894333517925 40.0 40.0 40.0 40.0 40.0 60-61 39.36575633590708 40.0 40.0 40.0 40.0 40.0 62-63 39.35676985307106 40.0 40.0 40.0 40.0 40.0 64-65 39.34822642350697 40.0 40.0 40.0 40.0 40.0 66-67 39.35682295563633 40.0 40.0 40.0 40.0 40.0 68-69 39.34937255091745 40.0 40.0 40.0 40.0 40.0 70-71 39.35093358779271 40.0 40.0 40.0 40.0 40.0 72-73 39.34880601302398 40.0 40.0 40.0 40.0 40.0 74-75 39.34594729413354 40.0 40.0 40.0 40.0 40.0 76-77 39.329399260114954 40.0 40.0 40.0 40.0 40.0 78-79 39.31832405127443 40.0 40.0 40.0 40.0 40.0 80-81 39.311506232693446 40.0 40.0 40.0 40.0 40.0 82-83 39.2874349202782 40.0 40.0 40.0 40.0 40.0 84-85 39.315021129858266 40.0 40.0 40.0 40.0 40.0 86-87 39.328412822237524 40.0 40.0 40.0 40.0 40.0 88-89 39.35603796042961 40.0 40.0 40.0 40.0 40.0 90-91 39.454660829509635 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19212117512466165 1101 2 0.01891325832884405 1101 3 0.30091704520822304 1101 4 0.12822935404673785 1101 5 0.13198739522036362 1101 6 0.05229031754186053 1101 7 0.07744378215137004 1101 8 -0.031267134355751125 1101 9 0.1096747719777511 1101 10-11 0.08594550095148179 1101 12-13 0.1349012988818643 1101 14-15 -0.07315902689025222 1101 16-17 0.010258940874876998 1101 18-19 -0.024852597800304466 1101 20-21 0.038799525736827434 1101 22-23 -0.059347283664251904 1101 24-25 -0.048633733772454946 1101 26-27 0.050929223667090184 1101 28-29 0.01225701621473263 1101 30-31 0.023472270001285267 1101 32-33 0.07860013492825146 1101 34-35 -0.03950272232501817 1101 36-37 0.09980586354198806 1101 38-39 0.07134718164783038 1101 40-41 0.026113992833728616 1101 42-43 0.0038952899393720486 1101 44-45 0.07653075298954803 1101 46-47 0.1706300472671316 1101 48-49 -0.039462032925897006 1101 50-51 0.12680128625533627 1101 52-53 0.020141141941522278 1101 54-55 0.021035053792431313 1101 56-57 0.13086235330104756 1101 58-59 0.15486117604054783 1101 60-61 0.07304452812486772 1101 62-63 -0.01810198266154117 1101 64-65 -0.034859663834581056 1101 66-67 -0.023361010688141448 1101 68-69 0.10612244746119615 1101 70-71 0.08204089906151069 1101 72-73 -0.04673030907562037 1101 74-75 -0.07079119948589607 1101 76-77 -0.02423937214435057 1101 78-79 -0.12683306632918345 1101 80-81 -0.14450106670629026 1101 82-83 -0.23663269505464513 1101 84-85 -0.36947401114324663 1101 86-87 -0.40651934160409553 1101 88-89 -0.6135760910134707 1101 90-91 0.2912911161553424 1102 1 0.3861773777058204 1102 2 0.0662706827985744 1102 3 0.2929818055410891 1102 4 0.08336078518045298 1102 5 0.11507224051062082 1102 6 0.20492621478205564 1102 7 0.04033514783783687 1102 8 0.14743078988858827 1102 9 0.2163354122826604 1102 10-11 0.02744964268387662 1102 12-13 0.049345247754786215 1102 14-15 8.733962080498259E-4 1102 16-17 0.04702650537404196 1102 18-19 0.042567125575381226 1102 20-21 0.10171378841781831 1102 22-23 0.10158460016211279 1102 24-25 0.11196542810214538 1102 26-27 0.024810550452514235 1102 28-29 0.05720667860331474 1102 30-31 0.07712159775983451 1102 32-33 0.04713227597488867 1102 34-35 0.10293016505030295 1102 36-37 -0.05781815370188781 1102 38-39 0.072035513330448 1102 40-41 0.02591740500230344 1102 42-43 0.10369279990922564 1102 44-45 0.12856363027717777 1102 46-47 -0.03065780728559986 1102 48-49 0.016565023410933577 1102 50-51 0.06690610520516316 1102 52-53 -0.01255667193512977 1102 54-55 -0.007782720957649758 1102 56-57 0.04363207150883852 1102 58-59 -0.048056783613837695 1102 60-61 0.04754437067214923 1102 62-63 -0.018412020206703517 1102 64-65 -0.13448416719562317 1102 66-67 -0.060568764898512484 1102 68-69 -0.1374476137106697 1102 70-71 -0.1941669649366915 1102 72-73 -0.001490045248239369 1102 74-75 0.11829942195286236 1102 76-77 0.006993825124453679 1102 78-79 0.18134653263593492 1102 80-81 -0.2155532630444057 1102 82-83 0.12363090291386669 1102 84-85 0.1177656700052836 1102 86-87 0.21064372022563305 1102 88-89 0.15536182662049214 1102 90-91 -0.025826000961771456 1103 1 0.3348310195713182 1103 2 0.34572181122013035 1103 3 0.16110308924170624 1103 4 -0.004573439762125986 1103 5 0.07811648294586604 1103 6 -0.0652901412957192 1103 7 0.058688015198043786 1103 8 0.020483476547752844 1103 9 -0.05874448341018734 1103 10-11 0.05501525656585926 1103 12-13 -0.047866015298126285 1103 14-15 -0.026869614834922118 1103 16-17 0.01990140793209605 1103 18-19 0.044195845818570945 1103 20-21 -0.04950479018445009 1103 22-23 0.07021209082867585 1103 24-25 0.06659937828747076 1103 26-27 -0.02530414752732213 1103 28-29 -1.3784662561988625E-4 1103 30-31 0.029772628672482426 1103 32-33 0.03321751695975195 1103 34-35 -0.03692950609848111 1103 36-37 -0.003739447341281732 1103 38-39 0.06601291293870304 1103 40-41 -0.07983898056025396 1103 42-43 -0.039897807356943815 1103 44-45 0.038815810187784905 1103 46-47 -0.03973015137722058 1103 48-49 -0.12067511124372743 1103 50-51 -0.07471123192837581 1103 52-53 -0.022108286955479173 1103 54-55 -0.09221809465238096 1103 56-57 -0.2331350310264071 1103 58-59 -0.10506615511525297 1103 60-61 -0.08128908632905052 1103 62-63 -0.22843651825416345 1103 64-65 -0.12334448438023315 1103 66-67 -0.15650538591195584 1103 68-69 -0.05055932119041273 1103 70-71 -0.035388550952063724 1103 72-73 -0.08451411643117979 1103 74-75 -0.028562111170813864 1103 76-77 0.1462115047512924 1103 78-79 0.12131989592826642 1103 80-81 0.17045702868860957 1103 82-83 0.04309553161242263 1103 84-85 0.09431394306282925 1103 86-87 0.10972491069934875 1103 88-89 0.27361760355088904 1103 90-91 0.3023791272433556 1104 1 0.34350688421834974 1104 2 0.2078139275712445 1104 3 0.12891179497730576 1104 4 0.05598543591145244 1104 5 0.09323569086299699 1104 6 0.04467771420976874 1104 7 0.18811878869101406 1104 8 0.06011304208719537 1104 9 0.11372934845217486 1104 10-11 0.047269031384530535 1104 12-13 0.0407559545264391 1104 14-15 0.024482999032187536 1104 16-17 -0.06612057581682507 1104 18-19 0.055218053455185157 1104 20-21 0.046082253449384325 1104 22-23 0.06907445247691157 1104 24-25 0.026333105046845162 1104 26-27 0.0470476872106218 1104 28-29 -0.027596588457697635 1104 30-31 0.023656305873238637 1104 32-33 0.03226250445080581 1104 34-35 -0.05064739687498587 1104 36-37 0.059060224236745285 1104 38-39 0.10279218339120888 1104 40-41 -0.008317104444714118 1104 42-43 -0.05282256153675746 1104 44-45 -0.024587848292391357 1104 46-47 0.004150674960008871 1104 48-49 0.017044110149015523 1104 50-51 0.04858735091744393 1104 52-53 0.14085764715973426 1104 54-55 0.1346808648392681 1104 56-57 0.007224826877063606 1104 58-59 0.015105744262861265 1104 60-61 0.018733962609445598 1104 62-63 -0.0766081922132642 1104 64-65 0.031784714244416534 1104 66-67 0.06767846734611282 1104 68-69 0.14951687578636808 1104 70-71 0.04961820602170519 1104 72-73 0.14769883896966007 1104 74-75 0.15983305909052348 1104 76-77 -0.13098333664866857 1104 78-79 0.256300523077428 1104 80-81 0.01174212956642151 1104 82-83 0.0038458341814262553 1104 84-85 0.09861863223993339 1104 86-87 -0.4262018293333867 1104 88-89 0.20052778210612843 1104 90-91 0.50750733237156 1201 1 0.25867649508385426 1201 2 0.12758560751713333 1201 3 0.08344070540408666 1201 4 0.06793758022551799 1201 5 0.12923232820077146 1201 6 0.15101433599046743 1201 7 0.10754500500620168 1201 8 0.11241713308321266 1201 9 0.08182555783005796 1201 10-11 -0.04980737047716133 1201 12-13 -0.038047328051035834 1201 14-15 -0.0034995550544607568 1201 16-17 -0.004806950361093243 1201 18-19 -0.060649663136764786 1201 20-21 0.0038863385568177478 1201 22-23 -5.117931353879612E-4 1201 24-25 -0.07685789091470241 1201 26-27 -0.04419228607202541 1201 28-29 0.08701994410814251 1201 30-31 0.04387434053288075 1201 32-33 0.007661052714283301 1201 34-35 -0.052678436324278266 1201 36-37 -0.02840255939525349 1201 38-39 0.005035899452877857 1201 40-41 0.026932372992803266 1201 42-43 0.013040493724872704 1201 44-45 -0.045195240714960505 1201 46-47 0.07206224948103568 1201 48-49 0.014161115703082316 1201 50-51 0.1248938561519779 1201 52-53 0.13575819294661073 1201 54-55 0.10649726699206497 1201 56-57 0.03568166646337545 1201 58-59 0.07961421240414523 1201 60-61 0.08311383825090957 1201 62-63 -0.018723808328090286 1201 64-65 -0.09391689429354244 1201 66-67 -0.1843543750548804 1201 68-69 -0.12748194969191928 1201 70-71 -0.1878887714139097 1201 72-73 -0.2114502181763811 1201 74-75 -0.5092110992276275 1201 76-77 -0.12482172548399006 1201 78-79 -0.08272888230069952 1201 80-81 0.0206607970733117 1201 82-83 0.02272198798056735 1201 84-85 -0.055983561371675705 1201 86-87 -0.23588692983539516 1201 88-89 -0.5940244103730379 1201 90-91 0.10072767135461191 1202 1 0.060342174791031766 1202 2 0.13364702333938538 1202 3 0.09171247060999832 1202 4 0.0279313560576 1202 5 -0.08042865808791078 1202 6 0.05454743102394133 1202 7 0.041621609557651595 1202 8 0.035158274228315634 1202 9 0.07492838801199042 1202 10-11 0.034637873065406666 1202 12-13 0.08978399479908461 1202 14-15 -0.017679881075494563 1202 16-17 0.008276448540442516 1202 18-19 0.08160281484634169 1202 20-21 -0.00485388875727466 1202 22-23 -0.056502912149504425 1202 24-25 -0.06767394420942452 1202 26-27 0.07229917466651159 1202 28-29 0.02903841097241866 1202 30-31 -0.02142828763851412 1202 32-33 -0.024388802772314477 1202 34-35 -0.040964747679289815 1202 36-37 0.03184153284949787 1202 38-39 -0.008806530169309212 1202 40-41 0.04165767820116173 1202 42-43 -0.060107293831755726 1202 44-45 -0.025354349552415556 1202 46-47 0.008443860497699518 1202 48-49 -3.0242064195817875E-4 1202 50-51 -0.023193228253333587 1202 52-53 0.050899180864988125 1202 54-55 0.002832155679641346 1202 56-57 0.06833857147653077 1202 58-59 0.03144503238302576 1202 60-61 -0.08534427067745298 1202 62-63 0.07867315118310358 1202 64-65 0.05385199133186802 1202 66-67 0.07699320204869764 1202 68-69 0.052152857784264484 1202 70-71 0.07910892151642201 1202 72-73 0.008256433740285729 1202 74-75 -0.10074125663053479 1202 76-77 0.09333279489389668 1202 78-79 0.10725040057010204 1202 80-81 -0.1443703435686885 1202 82-83 -0.29722920272325837 1202 84-85 -0.3005168061076944 1202 86-87 -0.14438412063043415 1202 88-89 -0.2756462096656094 1202 90-91 0.12655495141918038 1203 1 0.11094776703495768 1203 2 0.18803162894501213 1203 3 0.06662217708075246 1203 4 -0.006652504183136898 1203 5 -0.12724024938172818 1203 6 -0.06144044508758384 1203 7 0.029872410791455195 1203 8 -0.07202118207015218 1203 9 -0.02134759772303596 1203 10-11 -0.003850253954411187 1203 12-13 -0.06552435475964558 1203 14-15 -0.06591261691255568 1203 16-17 0.02789038432129587 1203 18-19 -0.03103372944399041 1203 20-21 -0.02980394128073982 1203 22-23 0.03552141591809743 1203 24-25 0.048229359081830125 1203 26-27 0.07190467992889182 1203 28-29 -0.017447200273622343 1203 30-31 -0.014418913704879799 1203 32-33 -0.03710947273545884 1203 34-35 0.026682354308306344 1203 36-37 0.006317848666242298 1203 38-39 0.029155179175191392 1203 40-41 0.02426033737347666 1203 42-43 0.01693358832441305 1203 44-45 0.08577675909367599 1203 46-47 -0.02103512022167564 1203 48-49 -0.01265300075587561 1203 50-51 -0.019775450808054984 1203 52-53 -0.013517312756448518 1203 54-55 -0.07963664674585402 1203 56-57 0.08353776638656996 1203 58-59 -0.06512547881228414 1203 60-61 -0.04058363888427863 1203 62-63 -0.1446490023049023 1203 64-65 -0.24757539848204146 1203 66-67 -0.011243005216329038 1203 68-69 -0.021756904487169493 1203 70-71 -0.055493536009279865 1203 72-73 -0.053166831558613126 1203 74-75 -0.16576805398904781 1203 76-77 -0.08236047516402323 1203 78-79 -0.14469372243234346 1203 80-81 -0.12022639576225203 1203 82-83 -0.13939731826885549 1203 84-85 0.02190181846788164 1203 86-87 -0.3165804019549512 1203 88-89 -0.01766725835435068 1203 90-91 -0.0176208727566447 1204 1 0.1577280894208002 1204 2 -0.020001605066759964 1204 3 -0.051210975969567585 1204 4 -0.09747797916804046 1204 5 -0.050734067775906055 1204 6 -0.037799701569475985 1204 7 -0.14304001148223477 1204 8 -0.045413798651324555 1204 9 -0.07768721109892596 1204 10-11 -0.1686888287674293 1204 12-13 -0.11411555916076566 1204 14-15 -0.11024604536453353 1204 16-17 -0.12445570597063238 1204 18-19 -0.12543584240512473 1204 20-21 -0.14587133837010668 1204 22-23 -0.11329740960997015 1204 24-25 -0.052913524605763484 1204 26-27 -0.04279566316184713 1204 28-29 -0.12043281966190023 1204 30-31 -0.045696578298837665 1204 32-33 -0.141689933544761 1204 34-35 0.006996056017484875 1204 36-37 -0.16849932347943053 1204 38-39 -0.1553002396138865 1204 40-41 -0.00544882273018743 1204 42-43 -0.11899508858372343 1204 44-45 -0.1611291008279565 1204 46-47 -0.10433610679631045 1204 48-49 -0.1411716648252721 1204 50-51 -0.2305746485168214 1204 52-53 -0.20279672685271066 1204 54-55 -0.23108961435053033 1204 56-57 -0.18301769347496588 1204 58-59 -0.23832080615559903 1204 60-61 -0.13779036167832714 1204 62-63 -0.044334597277206456 1204 64-65 -0.03930561667611698 1204 66-67 -0.1654024557207947 1204 68-69 -0.06115008820925283 1204 70-71 -0.28210235639966186 1204 72-73 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0.16398787782308943 2401 42-43 0.0539946055992786 2401 44-45 -0.018107258737664722 2401 46-47 0.08646064241350615 2401 48-49 0.06987511239631061 2401 50-51 0.019012176818115734 2401 52-53 0.08461160003076884 2401 54-55 0.09248589931096518 2401 56-57 0.15172114080459664 2401 58-59 0.13527918180172804 2401 60-61 0.09704404946727863 2401 62-63 0.08493307241452186 2401 64-65 0.12801266032124659 2401 66-67 0.10836380352037622 2401 68-69 0.1112800568270984 2401 70-71 0.1262843267302003 2401 72-73 0.16748796732778715 2401 74-75 0.20757442858484154 2401 76-77 0.17995631784020816 2401 78-79 0.02284202676334246 2401 80-81 0.1854303939902664 2401 82-83 0.19302262230558398 2401 84-85 0.4709186371441376 2401 86-87 0.3069950153792078 2401 88-89 0.34679462261026117 2401 90-91 0.19981502467925338 2402 1 0.3797415467565699 2402 2 0.3838363129971185 2402 3 0.34416084307302697 2402 4 0.3630097541389148 2402 5 0.12805140054909714 2402 6 0.34696764167102145 2402 7 0.24700693720433264 2402 8 0.2739246781025102 2402 9 0.1373234524944067 2402 10-11 0.19025020076460208 2402 12-13 0.1974752039100096 2402 14-15 0.1486754830363637 2402 16-17 0.11263096638146664 2402 18-19 0.04083309788675393 2402 20-21 0.06625056549541597 2402 22-23 0.06230312599877408 2402 24-25 0.12726696141839966 2402 26-27 0.12362780216747637 2402 28-29 0.06843072477956014 2402 30-31 0.11468558240992621 2402 32-33 0.09535050269902712 2402 34-35 0.14016459190347064 2402 36-37 0.013070188158245344 2402 38-39 0.033671146586101486 2402 40-41 0.12790222231044623 2402 42-43 0.0602679751859867 2402 44-45 0.044992771966327894 2402 46-47 0.12035149460037076 2402 48-49 0.10406304555502288 2402 50-51 0.12626107077598192 2402 52-53 0.07373839052884534 2402 54-55 0.13780035399792467 2402 56-57 0.08465432676090501 2402 58-59 0.08302875390767639 2402 60-61 0.10601953754798643 2402 62-63 0.1962643823449568 2402 64-65 0.09854069572943303 2402 66-67 0.14450837602803546 2402 68-69 0.12062427622819172 2402 70-71 0.12489989950084635 2402 72-73 0.014687034401546839 2402 74-75 0.12737281871949335 2402 76-77 0.14356594514940468 2402 78-79 0.0776167425137686 2402 80-81 0.3269130442758197 2402 82-83 0.3498722873580675 2402 84-85 0.19814456170962558 2402 86-87 -0.12324417168465374 2402 88-89 0.3658660016816384 2402 90-91 0.35656393614514315 2403 1 -0.3228631721198383 2403 2 -0.2598390573022229 2403 3 0.011083638205121815 2403 4 0.09701324006885415 2403 5 0.06953530126078533 2403 6 0.10995884333538442 2403 7 0.04078521088125342 2403 8 0.10145680931088208 2403 9 0.11097020097582089 2403 10-11 0.05193738568892314 2403 12-13 0.07384479168953817 2403 14-15 0.1259355799863755 2403 16-17 0.05372658494893301 2403 18-19 0.053470777698620964 2403 20-21 0.02269150765213368 2403 22-23 0.0571357880172485 2403 24-25 0.10514693230154393 2403 26-27 0.10693769651771134 2403 28-29 0.062415364820061825 2403 30-31 0.06531090176338239 2403 32-33 0.03446638475690378 2403 34-35 0.03247789755328512 2403 36-37 0.0698241365913077 2403 38-39 0.10084843513978115 2403 40-41 0.03207399458548821 2403 42-43 0.05411500535485203 2403 44-45 0.01649518460785515 2403 46-47 0.016512851329672174 2403 48-49 0.04180087836699897 2403 50-51 -0.0010360781819542808 2403 52-53 -0.0885774721764534 2403 54-55 0.0135569466286114 2403 56-57 0.03408771793465348 2403 58-59 0.07387612210746397 2403 60-61 0.020547329326994657 2403 62-63 0.24651720501619678 2403 64-65 0.2401764868339029 2403 66-67 0.21146379689373873 2403 68-69 0.014355487568415981 2403 70-71 0.12681724560948737 2403 72-73 0.10724444286923074 2403 74-75 0.2381369639571531 2403 76-77 0.22483905977215812 2403 78-79 0.13219678688783176 2403 80-81 0.12208342615844714 2403 82-83 0.35100209693133166 2403 84-85 -0.01620247352056481 2403 86-87 0.25440871369753637 2403 88-89 0.28252111833675286 2403 90-91 -0.1619545823733759 2404 1 -0.3868469149360436 2404 2 -0.4094074504664249 2404 3 -0.5352036698403495 2404 4 -0.34024166436923053 2404 5 -0.12631345111462622 2404 6 -0.2118976830308128 2404 7 -0.15247941743261606 2404 8 -0.04151678918996993 2404 9 0.0066573894451948945 2404 10-11 0.013765122824210607 2404 12-13 0.10792159413642821 2404 14-15 0.052177705167295585 2404 16-17 0.05728129971893736 2404 18-19 0.04647617939949811 2404 20-21 0.03374422515872055 2404 22-23 0.05229266110922026 2404 24-25 0.02450479824856444 2404 26-27 0.019603062980280583 2404 28-29 0.008231864777876297 2404 30-31 -0.03139469100937475 2404 32-33 0.01794496736143003 2404 34-35 0.04335954897706529 2404 36-37 0.09252042718108555 2404 38-39 0.13113656101858595 2404 40-41 0.020892107376248248 2404 42-43 0.10546058742524878 2404 44-45 0.07497572665025842 2404 46-47 0.010903419657530833 2404 48-49 0.052855129734041384 2404 50-51 -0.004375274164551968 2404 52-53 -0.019198293109170095 2404 54-55 0.04369618012846388 2404 56-57 0.05610383678893527 2404 58-59 0.1253363607520086 2404 60-61 0.022787619792381975 2404 62-63 0.13517602933514894 2404 64-65 0.07671357310888993 2404 66-67 0.07273704554673088 2404 68-69 0.1225433626304806 2404 70-71 0.23673108643725982 2404 72-73 0.09209070390197382 2404 74-75 0.25471372599466235 2404 76-77 0.22117865817071447 2404 78-79 0.38071828207472436 2404 80-81 0.2202509744800878 2404 82-83 0.23403530313630938 2404 84-85 0.12336623554087822 2404 86-87 -0.02842329334401228 2404 88-89 0.18952966534530447 2404 90-91 -0.21776739290316982 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 10.0 31 377.0 32 2809.0 33 4982.0 34 6533.0 35 8357.0 36 11888.0 37 18264.0 38 47818.0 39 311886.0 40 287612.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.524213982370366 13.984797116448377 26.89554262874273 31.595446272438526 2 25.925513507957447 19.37011403674073 27.57125154894672 27.133120906355106 3 25.506511526322328 20.02235459875208 22.02099276687408 32.45014110805151 4 26.3276976486576 21.829142256786234 20.383106649765324 31.460053444790848 5 26.636032980460676 22.027989996231454 20.69329770347277 30.642679319835096 6 26.544817111468934 24.93305126360387 23.669590142405244 24.852541482521957 7 24.605016730046707 24.615009078762547 24.021320817202827 26.75865337398792 8 23.54197357452008 26.052337067616797 24.7370584809346 25.66863087692852 9 23.365679993604896 26.109578950974683 25.241386595406944 25.283354460013474 10-11 23.32678120753252 24.88872805965718 25.13525357726084 26.649237155549464 12-13 23.789855196592324 24.513087121860973 24.903288339214544 26.79376934233216 14-15 24.29040049333653 24.00683191156486 24.801080886635376 26.901686708463235 16-17 24.23151700983247 23.80448685006909 24.58795836331038 27.37603777678806 18-19 24.317665330546895 24.00483344182169 24.55334201240193 27.124159215229483 20-21 24.442783811253097 24.21788459122729 24.21039032969041 27.128941267829205 22-23 24.320020669887057 23.860586750716596 24.44635250722304 27.373040072173303 24-25 24.436217410668405 24.151078602669955 24.35991869083102 27.052785295830624 26-27 24.649411307912796 24.147224411022417 24.256997499057864 26.94636678200692 28-29 24.610227026162825 23.824899791017167 24.313525643221762 27.251347539598253 30-31 24.737718502488455 24.05445548441157 24.2501629110873 26.957663102012674 32-33 24.877010968392035 24.11265476783377 24.157680769988072 26.85265349378612 34-35 24.714979647479403 23.82047471708776 24.318210901376634 27.146334734056204 36-37 24.83279315770804 24.111363092007725 24.273491575988913 26.78235217429532 38-39 25.002980775749812 24.138906487804537 24.20265222802272 26.655460508422934 40-41 24.898321080906246 23.931053848190054 24.309850565177896 26.8607745057258 42-43 25.020678798228296 24.106559181386412 24.33452625540317 26.538235764982122 44-45 25.076085938069344 24.11429721579256 24.202702655806995 26.6069141903311 46-47 25.09192442977844 23.782227943576167 24.34447184247743 26.781375784167967 48-49 25.10636193208245 24.153028021720377 24.29931431865112 26.441295727546056 50-51 25.22104790782049 24.261228692804764 24.108370821576 26.409352577798746 52-53 25.26535880664838 23.905924792397006 24.288384829615058 26.540331571339554 54-55 25.393137975805335 24.12113322830886 24.16133380327843 26.324394992607374 56-57 25.52962194490466 24.099795876439767 24.0597881392077 26.31079403944787 58-59 25.571312542705016 23.877808862016064 24.091301713357485 26.459576881921436 60-61 25.742583897098093 24.12723095068542 24.00650369414278 26.123681458073705 62-63 25.753825195620756 24.202602839653657 23.848892124272982 26.194679840452608 64-65 25.83873419516305 23.93564140145976 23.790391054820876 26.43523334855632 66-67 25.99781806583497 24.144080144399098 23.79341215359077 26.064689636175164 68-69 26.13036785498538 24.105148444508668 23.602606634595507 26.161877065910446 70-71 26.2283977615539 23.94217721405421 23.490002130785363 26.339422893606525 72-73 26.217425905180935 24.027402537497274 23.451320704984262 26.30385085233753 74-75 26.367599982618017 24.1311180960934 23.21421846256355 26.28706345872503 76-77 26.542688365674277 23.975496053450918 23.183493495346607 26.2983220855282 78-79 26.850881372081943 23.991980308083214 22.877560743211053 26.27957757662379 80-81 27.235183515281747 23.874733792521248 22.555687726636098 26.334394965560907 82-83 27.76845436455611 23.472225552012656 22.108077006065546 26.651243077365685 84-85 28.86805931717733 22.831932047447463 21.374908739784743 26.925099895590463 86-87 29.316944727176175 22.15117050935965 20.67641722352525 27.855467539938928 88-89 28.750901225666908 21.49062725306417 20.44340302811824 29.315068493150687 90-91 26.570907828501994 23.40305502516924 22.760805415726438 27.265231730602324 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.5 12 4.0 13 6.0 14 5.5 15 6.5 16 11.5 17 22.5 18 37.5 19 54.0 20 78.5 21 117.0 22 169.5 23 255.5 24 380.5 25 537.0 26 768.0 27 1077.5 28 1473.0 29 1941.5 30 2574.0 31 3448.5 32 4520.0 33 5700.5 34 7341.0 35 9434.0 36 11580.5 37 13869.5 38 16675.5 39 20110.0 40 23736.5 41 27493.5 42 31425.0 43 35365.5 44 39036.0 45 42498.5 46 45685.5 47 48228.5 48 50052.0 49 51308.5 50 51938.0 51 51937.0 52 51382.0 53 50161.0 54 48149.0 55 45893.0 56 43623.0 57 40878.0 58 38197.5 59 35739.0 60 33277.0 61 30894.0 62 28647.5 63 26587.5 64 24556.0 65 22441.5 66 19949.5 67 17535.0 68 15456.0 69 13355.0 70 11348.0 71 9480.0 72 7652.5 73 5951.5 74 4604.5 75 3569.0 76 2730.5 77 2004.5 78 1455.0 79 1051.5 80 735.0 81 507.5 82 355.0 83 252.5 84 168.5 85 103.0 86 57.0 87 32.5 88 22.5 89 17.5 90 17.5 91 16.0 92 9.0 93 4.0 94 2.5 95 2.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0015702262267749266 2 0.008564870327863235 3 0.0012847305491794855 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.8122343943965713E-4 68-69 2.0069520820120896E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 6388.0 32-33 14256.0 34-35 15936.0 36-37 17518.0 38-39 19714.0 40-41 21361.0 42-43 23098.0 44-45 24334.0 46-47 25640.0 48-49 26353.0 50-51 27257.0 52-53 27632.0 54-55 27841.0 56-57 28337.0 58-59 28265.0 60-61 28501.0 62-63 28031.0 64-65 27376.0 66-67 26990.0 68-69 26309.0 70-71 25744.0 72-73 24970.0 74-75 24342.0 76-77 22820.0 78-79 21938.0 80-81 21137.0 82-83 19905.0 84-85 18881.0 86-87 17674.0 88-89 16576.0 90-91 15412.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.7342131291608 #Duplication Level Percentage of deduplicated Percentage of total 1 99.74636147183837 99.48124873890642 2 0.24393623261567654 0.4865757642723286 3 0.007347337508101149 0.02198342774894512 4 0.001555559744051406 0.00620570108193463 5 7.993982937900116E-4 0.003986367990397027 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.13739193988603E-4 0.0 0.0 0.0 0.0 2 7.13739193988603E-4 0.0 0.0 0.0 0.0 3 7.13739193988603E-4 0.0 0.0 0.0 0.0 4 7.13739193988603E-4 0.0 0.0 0.0 0.0 5 7.13739193988603E-4 0.0 0.0 0.0 0.0 6 7.13739193988603E-4 0.0 0.0 0.0 0.0 7 0.0011419827103817649 0.0 0.0 0.0 0.0 8 0.007708383295076913 0.0 0.0 0.0 0.0 9 0.008279374650267795 0.0 0.0 0.0 0.0 10-11 0.008422122489065516 0.0 0.0 0.0 1.427478387977206E-4 12-13 0.008422122489065516 0.0 0.0 0.0 1.427478387977206E-4 14-15 0.008422122489065516 0.0 0.0 0.0 1.427478387977206E-4 16-17 0.008422122489065516 0.0 0.0 0.0 1.427478387977206E-4 18-19 0.008422122489065516 0.0 0.0 0.0 1.427478387977206E-4 20-21 0.008493496408464377 0.0 0.0 0.0 1.427478387977206E-4 22-23 0.008636244247262096 0.0 0.0 0.0 1.427478387977206E-4 24-25 0.008707618166660956 0.0 0.0 0.0 1.427478387977206E-4 26-27 0.008707618166660956 0.0 0.0 0.0 1.427478387977206E-4 28-29 0.008778992086059817 0.0 0.0 0.0 3.5686959699430154E-4 30-31 0.008850366005458677 0.0 0.0 0.0 4.282435163931618E-4 32-33 0.008850366005458677 0.0 0.0 0.0 4.282435163931618E-4 34-35 0.00949273128004842 0.0 0.0 0.0 5.709913551908824E-4 36-37 0.009778226957643861 0.0 0.0 0.0 5.709913551908824E-4 38-39 0.009920974796441582 0.0 0.0 0.0 5.709913551908824E-4 40-41 0.009992348715840442 0.0 0.0 0.0 6.423652745897426E-4 42-43 0.009992348715840442 0.0 0.0 7.13739193988603E-5 7.13739193988603E-4 44-45 0.010135096554638163 0.0 0.0 1.427478387977206E-4 7.13739193988603E-4 46-47 0.010420592232233603 0.0 0.0 1.427478387977206E-4 7.851131133874633E-4 48-49 0.010563340071031324 0.0 0.0 2.141217581965809E-4 8.564870327863236E-4 50-51 0.010706087909829045 0.0 0.0 2.854956775954412E-4 8.564870327863236E-4 52-53 0.010706087909829045 0.0 0.0 2.854956775954412E-4 8.564870327863236E-4 54-55 0.010848835748626766 7.13739193988603E-5 0.0 2.854956775954412E-4 8.564870327863236E-4 56-57 0.011062957506823347 1.427478387977206E-4 0.0 2.854956775954412E-4 8.564870327863236E-4 58-59 0.011134331426222208 2.141217581965809E-4 0.0 2.854956775954412E-4 8.564870327863236E-4 60-61 0.011277079265019927 2.854956775954412E-4 0.0 2.854956775954412E-4 8.564870327863236E-4 62-63 0.011277079265019927 2.854956775954412E-4 0.0 2.854956775954412E-4 9.992348715840442E-4 64-65 0.011277079265019927 2.854956775954412E-4 0.0 2.854956775954412E-4 0.0010706087909829044 66-67 0.011348453184418787 2.854956775954412E-4 0.0 2.854956775954412E-4 0.0011419827103817649 68-69 0.011419827103817648 2.854956775954412E-4 0.0 2.854956775954412E-4 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