##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-29C_S28_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1204397
Sequences flagged as poor quality	0
Sequence length	61
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.41748775528335	40.0	40.0	40.0	40.0	40.0
2	39.26432895465532	40.0	40.0	40.0	40.0	40.0
3	39.14251530018756	40.0	40.0	40.0	40.0	40.0
4	39.09201367987466	40.0	40.0	40.0	40.0	40.0
5	39.082100005230835	40.0	40.0	40.0	40.0	40.0
6	39.20046629143048	40.0	40.0	40.0	40.0	40.0
7	39.25984206204433	40.0	40.0	40.0	40.0	40.0
8	39.24951656306019	40.0	40.0	40.0	40.0	40.0
9	39.254548126572885	40.0	40.0	40.0	40.0	40.0
10	39.28941370661003	40.0	40.0	40.0	40.0	40.0
11	39.251881231852956	40.0	40.0	40.0	40.0	40.0
12	39.25073875142499	40.0	40.0	40.0	40.0	40.0
13	39.177390843716815	40.0	40.0	40.0	40.0	40.0
14	39.297706653204884	40.0	40.0	40.0	40.0	40.0
15	39.252246559896776	40.0	40.0	40.0	40.0	40.0
16	39.26872617583737	40.0	40.0	40.0	40.0	40.0
17	39.30224004211236	40.0	40.0	40.0	40.0	40.0
18	39.25769326891382	40.0	40.0	40.0	40.0	40.0
19	39.309181274945054	40.0	40.0	40.0	40.0	40.0
20	38.92931649613873	40.0	40.0	40.0	40.0	40.0
21	39.14662690126262	40.0	40.0	40.0	40.0	40.0
22	39.145796610253925	40.0	40.0	40.0	40.0	40.0
23	39.268998511288224	40.0	40.0	40.0	40.0	40.0
24	39.28593312670158	40.0	40.0	40.0	40.0	40.0
25	39.03393648439842	40.0	40.0	40.0	40.0	40.0
26	39.239964895296154	40.0	40.0	40.0	40.0	40.0
27	39.25110407946881	40.0	40.0	40.0	40.0	40.0
28	38.9757762598213	40.0	40.0	40.0	40.0	40.0
29	39.20146264064092	40.0	40.0	40.0	40.0	40.0
30	38.83315551267564	40.0	40.0	40.0	40.0	40.0
31	39.02154522138464	40.0	40.0	40.0	40.0	40.0
32	39.074856546470976	40.0	40.0	40.0	40.0	40.0
33	39.10295359420523	40.0	40.0	40.0	40.0	40.0
34	38.92035267440885	40.0	40.0	40.0	40.0	40.0
35	39.04431844317115	40.0	40.0	40.0	40.0	40.0
36	38.95673934757393	40.0	40.0	40.0	40.0	40.0
37	38.9542252263996	40.0	40.0	40.0	40.0	40.0
38	38.77104310289713	40.0	40.0	40.0	40.0	40.0
39	39.00538443719139	40.0	40.0	40.0	40.0	40.0
40	38.74890090227724	40.0	40.0	40.0	40.0	40.0
41	38.73376967893477	40.0	40.0	40.0	40.0	40.0
42	38.5012317367114	40.0	40.0	40.0	40.0	40.0
43	38.719126666705414	40.0	40.0	40.0	40.0	40.0
44	38.32543920318633	40.0	40.0	40.0	40.0	40.0
45	38.67493857922263	40.0	40.0	40.0	40.0	40.0
46	38.2871860358337	40.0	40.0	40.0	40.0	40.0
47	37.78648402478585	40.0	40.0	40.0	24.0	40.0
48	38.18528940208254	40.0	40.0	40.0	40.0	40.0
49	37.79837047086633	40.0	40.0	40.0	24.0	40.0
50	38.111337042520034	40.0	40.0	40.0	40.0	40.0
51	37.725364643053744	40.0	40.0	40.0	24.0	40.0
52	37.77762315914105	40.0	40.0	40.0	24.0	40.0
53	37.04118492490433	40.0	40.0	40.0	24.0	40.0
54	36.390522394193944	40.0	40.0	40.0	24.0	40.0
55	36.329791588653904	40.0	40.0	40.0	24.0	40.0
56	36.19082080078247	40.0	40.0	40.0	24.0	40.0
57	36.279549019135715	40.0	40.0	40.0	24.0	40.0
58	36.24899098885168	40.0	40.0	40.0	24.0	40.0
59	35.8254462606599	40.0	40.0	40.0	24.0	40.0
60	35.19871271682012	40.0	40.0	40.0	12.0	40.0
61	35.073308884030766	40.0	40.0	40.0	12.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.0067089011098318
1101	2	0.02054906452325156
1101	3	-5.763954752424638E-4
1101	4	-0.18446947963884952
1101	5	0.07687107401113735
1101	6	-0.06311899356033024
1101	7	-0.03913020477137508
1101	8	-0.05482477321213963
1101	9	0.009517819141450445
1101	10	0.007017498290650792
1101	11	-0.06155159089153983
1101	12	0.011323327636404201
1101	13	-0.12170647765067599
1101	14	0.04467171250782087
1101	15	-0.02918977684818458
1101	16	-0.0043506082626478815
1101	17	0.0506608438585161
1101	18	-0.020760787145007953
1101	19	-0.018319156124157132
1101	20	-0.05394727204742367
1101	21	-0.05086922925262627
1101	22	0.01469750008021009
1101	23	0.07251410502939848
1101	24	-8.025754017779718E-4
1101	25	0.056970583296440225
1101	26	-0.01797835334225084
1101	27	0.08900467375068644
1101	28	-5.731805687361202E-4
1101	29	-0.050746685464659436
1101	30	-0.027306567743210053
1101	31	0.0954807124993522
1101	32	0.10228323003993722
1101	33	0.013951424748078978
1101	34	0.039435711066985846
1101	35	0.03222911290224317
1101	36	0.1314701694990532
1101	37	-0.06663928835902766
1101	38	0.08859124944775942
1101	39	-0.07557425963378961
1101	40	0.12594403861838543
1101	41	0.003294322957906104
1101	42	0.1073727801519837
1101	43	0.0730269626165807
1101	44	-0.09010862952821697
1101	45	0.05579057447027935
1101	46	0.10425863755065023
1101	47	-0.21781758027606202
1101	48	0.25659105897980083
1101	49	-0.016286616681441046
1101	50	-0.03732628499955837
1101	51	0.12264301251487097
1101	52	0.070124121360152
1101	53	0.14241386352629348
1101	54	0.20571128510042058
1101	55	-0.10029360233089335
1101	56	0.19539111742584225
1101	57	-0.12761878895261702
1101	58	0.33324059312128895
1101	59	0.05177695274168315
1101	60	0.04760209539818305
1101	61	0.07993472820532332
1102	1	-0.03174855824490663
1102	2	-0.11496899541204186
1102	3	-0.05792949890620491
1102	4	0.023470932583940396
1102	5	-0.11617847213681642
1102	6	-0.2551339195744191
1102	7	-0.11911677981535718
1102	8	-0.030975391704878064
1102	9	-0.0817864166568043
1102	10	-0.08635227798430378
1102	11	-0.04695406539106273
1102	12	-0.08820897913705039
1102	13	-0.016611799337596267
1102	14	-0.010673461478148738
1102	15	-0.02487925602991936
1102	16	-0.0154735014463796
1102	17	-0.027306173825827784
1102	18	0.05961849428950927
1102	19	-0.045894755738054016
1102	20	-0.057772327428672554
1102	21	0.03366280333988669
1102	22	-0.05499440642871889
1102	23	-0.08151850202173705
1102	24	-0.025289642319769712
1102	25	0.013003588075115147
1102	26	-0.0815156574763023
1102	27	-0.1082018518878698
1102	28	0.010999765766456449
1102	29	-0.001182699230525941
1102	30	0.07374529605955615
1102	31	-0.09859182586781401
1102	32	-0.014699669658064352
1102	33	0.1380521938421353
1102	34	0.08185170103526929
1102	35	-0.027158082781689075
1102	36	-0.021468885032561502
1102	37	-0.07766334426815291
1102	38	0.008699019074981607
1102	39	-0.055766635553702315
1102	40	0.004928895241569364
1102	41	0.05399599493311058
1102	42	0.023428750062109316
1102	43	-0.00993556576774779
1102	44	0.0861367300419218
1102	45	0.038639136077414094
1102	46	-0.12046191260827754
1102	47	-0.05888116448826253
1102	48	0.12951407227460265
1102	49	0.204274575549789
1102	50	-0.04711408216380164
1102	51	-0.056710435789469216
1102	52	0.16731049635115625
1102	53	0.0464999523780989
1102	54	-0.07508779048036018
1102	55	0.1874978140217891
1102	56	-0.0976910000967024
1102	57	0.1725233226634728
1102	58	-0.02362618599259747
1102	59	0.26872159924316463
1102	60	0.06732990970038344
1102	61	-0.02572654667660146
1103	1	0.09592208828497917
1103	2	0.010183855779828832
1103	3	0.04511827360855847
1103	4	-0.04513958852273703
1103	5	-0.008832822182547773
1103	6	0.04031823782806754
1103	7	-0.01889783848967852
1103	8	0.04386860686834382
1103	9	0.007213700779168164
1103	10	-0.012651136781805405
1103	11	-0.014584006100847091
1103	12	0.08462168038456497
1103	13	0.05175148814387143
1103	14	0.008727997878132498
1103	15	0.03655590725094271
1103	16	0.05135693755879345
1103	17	-0.0327365407743585
1103	18	-0.061459787253369313
1103	19	0.031285999357301364
1103	20	0.10387938459439994
1103	21	0.023341995779162517
1103	22	0.05321257357466891
1103	23	0.07480014144723413
1103	24	0.04249224737854718
1103	25	-0.03645111224483344
1103	26	0.06118998357756311
1103	27	0.10364227369165491
1103	28	0.09880678157884404
1103	29	0.12933352657272934
1103	30	-0.03026349748222401
1103	31	0.09344649374904179
1103	32	0.01569172310265543
1103	33	0.05942591956713272
1103	34	0.1056539189202681
1103	35	0.07431297722649788
1103	36	0.11362036530922381
1103	37	0.008503156811798362
1103	38	0.029060701930468724
1103	39	0.0039075862934225825
1103	40	-0.06800263161149189
1103	41	0.21186822260178673
1103	42	0.10333944289646269
1103	43	0.09282669178628566
1103	44	0.22312489413087633
1103	45	0.045170888949861876
1103	46	0.03777717785909118
1103	47	0.17636482000899179
1103	48	0.30947463053610136
1103	49	0.22299893378413316
1103	50	0.05161587471586415
1103	51	0.21855150723953187
1103	52	0.13283416607972498
1103	53	0.02853324557584358
1103	54	0.0013026145421477509
1103	55	0.32605312028344
1103	56	0.3714443740704567
1103	57	0.3956245534682239
1103	58	0.24869925959117722
1103	59	0.2102907232725002
1103	60	0.2747804545016095
1103	61	0.2526640980030237
1104	1	-0.008187820480593189
1104	2	0.07881902357165416
1104	3	0.04433452776429192
1104	4	0.0414295600012764
1104	5	-0.05233664584081055
1104	6	-0.010288349922291218
1104	7	0.0014752412420406813
1104	8	0.013572032643530463
1104	9	0.02978062204195453
1104	10	-0.015990950572096096
1104	11	-0.025477210892319135
1104	12	-0.015104318510701376
1104	13	-0.03624958667677447
1104	14	-0.026693648896397804
1104	15	0.035386487299675196
1104	16	0.009853983621241014
1104	17	-0.09649324105343027
1104	18	0.050291643493807214
1104	19	0.10555427302636389
1104	20	-0.012797948077007959
1104	21	0.05347281907749135
1104	22	0.11081600653239576
1104	23	0.03335971468845855
1104	24	-0.012653220620542527
1104	25	0.0496568983357335
1104	26	0.06425974463883932
1104	27	0.010107004522254215
1104	28	0.0688438653166159
1104	29	0.04086861867163094
1104	30	0.054500178754743445
1104	31	0.006654457393800328
1104	32	0.06408209968471823
1104	33	-0.036480261635283284
1104	34	-0.02364716602061634
1104	35	-0.05950354027107352
1104	36	0.03362357791255022
1104	37	0.09139074817188941
1104	38	-0.031066187815383728
1104	39	0.09959087966461055
1104	40	0.0835354898214149
1104	41	0.027637550136802247
1104	42	0.11689948576530895
1104	43	0.006839962371437025
1104	44	0.057865291121864004
1104	45	0.13246718544080238
1104	46	-0.1510414263483142
1104	47	-0.03384751231811123
1104	48	-0.34795795768617666
1104	49	0.02472767552033872
1104	50	0.14932941480124384
1104	51	0.11976179894364236
1104	52	-0.1403796891345479
1104	53	-0.0028562067089836773
1104	54	0.13240189490014131
1104	55	0.03274108931307751
1104	56	0.302710294750149
1104	57	0.1328414175334629
1104	58	0.0710126493148664
1104	59	-0.051100643907169285
1104	60	0.1258803331800209
1104	61	-0.04860694208943528
1201	1	0.026687325052243693
1201	2	-0.025136071715493813
1201	3	0.10088730436638826
1201	4	0.11981219441000945
1201	5	0.05515065342037673
1201	6	0.03789413079180548
1201	7	0.07478600696155979
1201	8	0.07227881757949461
1201	9	0.0968490041952137
1201	10	0.11888520074454334
1201	11	0.01337213981987162
1201	12	-0.059701315892674245
1201	13	0.07450895643723499
1201	14	0.07270823594244291
1201	15	-0.03733929281477799
1201	16	-0.019052242805699393
1201	17	-0.052442628925255974
1201	18	0.08191946651826498
1201	19	-0.015029418651430149
1201	20	0.03511487384516698
1201	21	0.042194992505606876
1201	22	-0.08087170683987921
1201	23	0.02115493601934304
1201	24	0.012664804139681962
1201	25	-0.017042877646311183
1201	26	0.030150082128237443
1201	27	0.09591993471569538
1201	28	0.04801870397665908
1201	29	-0.004787985535863015
1201	30	0.005349427257549166
1201	31	0.009833821537277743
1201	32	-0.027579599225475704
1201	33	0.07209926446657278
1201	34	0.08161762287488017
1201	35	0.06358863057190689
1201	36	0.05969349402202795
1201	37	-0.00245105508429333
1201	38	0.14498077195842995
1201	39	0.14456083255315377
1201	40	-0.022017442541262255
1201	41	0.06805918557931534
1201	42	0.20415639529614538
1201	43	0.05769130399188782
1201	44	0.16258858776583196
1201	45	0.05506804448890534
1201	46	0.19025472451708225
1201	47	0.6763897085870809
1201	48	0.24030704622958865
1201	49	0.18939894867681062
1201	50	0.003550567086968215
1201	51	0.1696954308044809
1201	52	0.14927843407831887
1201	53	-7.114407127346567E-4
1201	54	0.04530319244279468
1201	55	0.11655604357845561
1201	56	0.4920580657021887
1201	57	0.30645383684310445
1201	58	-0.010044826336333301
1201	59	0.09784615094527993
1201	60	0.547703340783471
1201	61	0.3050701355269041
1202	1	-0.21685437074975056
1202	2	-0.1867377244395172
1202	3	-0.06904755742499447
1202	4	-0.06695029542872533
1202	5	-0.025603690750159558
1202	6	-0.003909480337107141
1202	7	-0.03228128783266726
1202	8	-0.014027864665422385
1202	9	-4.863194353532663E-5
1202	10	0.032542246269287034
1202	11	0.030750031035694292
1202	12	-0.030149389440936147
1202	13	-0.018297709485175062
1202	14	-7.991852887130335E-4
1202	15	0.0209246564438601
1202	16	-0.08290990734963088
1202	17	0.017590450005407376
1202	18	-0.026243786998698226
1202	19	-0.010315909489129638
1202	20	-0.09407525726013688
1202	21	-0.19982595645385715
1202	22	-0.09599050981329071
1202	23	0.03115075665235878
1202	24	-0.09790423864019004
1202	25	-0.04564877059406314
1202	26	0.0630326053750423
1202	27	-0.02036588024944308
1202	28	-0.06217821258820777
1202	29	-0.058466050146378734
1202	30	0.03647153825836824
1202	31	-0.08634938600530262
1202	32	0.15231239281989417
1202	33	0.007221409858843231
1202	34	0.10657855969792251
1202	35	0.06867749014745783
1202	36	-0.0823848322321794
1202	37	0.04468242443955717
1202	38	0.05254300805276557
1202	39	0.043019544880912974
1202	40	-0.05842643262698033
1202	41	0.06382446985755053
1202	42	-0.05580426142467587
1202	43	0.11414217909974411
1202	44	0.08903253487769547
1202	45	-0.004174986305820028
1202	46	-0.11600568779607556
1202	47	-0.13565202704720747
1202	48	0.26410995851352936
1202	49	0.01888588066544372
1202	50	0.05502247404305649
1202	51	0.08067800387858881
1202	52	0.04358142354150374
1202	53	-0.23244907802831705
1202	54	-0.16407382394056924
1202	55	-0.15452352950007509
1202	56	0.20329161574194643
1202	57	-0.0037379478728851723
1202	58	0.15193244548413531
1202	59	0.026816526071343105
1202	60	0.16984611338708078
1202	61	-0.18535083863504553
1203	1	0.09222786411803696
1203	2	-0.004071516087911675
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2403	7	0.02077753196137877
2403	8	-0.0035909712432697916
2403	9	-0.01701494771393186
2403	10	0.0595021918546621
2403	11	0.03153777853232498
2403	12	0.053169780624052976
2403	13	0.05712600051085559
2403	14	-0.03829148368730273
2403	15	0.09625650406936614
2403	16	-0.013047191096930533
2403	17	-0.029184928306044355
2403	18	-0.056811267484341954
2403	19	-0.015487594933283333
2403	20	-0.11800595816933424
2403	21	0.018789330371525637
2403	22	0.08925881455211737
2403	23	-0.026887999722063682
2403	24	-0.158841086788307
2403	25	0.018727659264833107
2403	26	-0.05924893771079809
2403	27	-0.061355589381221876
2403	28	-0.07150141311470293
2403	29	-0.03316128739374591
2403	30	-0.1151921287006985
2403	31	-0.0023561896312926933
2403	32	0.05477779761024948
2403	33	-0.0661741339446067
2403	34	-0.06311143731308988
2403	35	-0.03287387022149346
2403	36	-0.10077833507233436
2403	37	-0.10762815282758709
2403	38	-0.054913553255097725
2403	39	-0.12578503169131494
2403	40	-0.11599065898499816
2403	41	0.0017379586084516063
2403	42	-0.015490792153016741
2403	43	-0.053537083433575106
2403	44	-0.16846101002684577
2403	45	0.1011715074188686
2403	46	-0.015338641090352212
2403	47	-0.1932154233235579
2403	48	-0.37455459504192135
2403	49	-0.1967864899765388
2403	50	0.11096306067145179
2403	51	0.031139560410366585
2403	52	-0.11881353913560133
2403	53	-0.2807471028005821
2403	54	-0.03645983626216065
2403	55	-0.29529735514377364
2403	56	-0.19679778405298265
2403	57	-0.5615279218560474
2403	58	-0.4502774117494326
2403	59	-0.27429891691314623
2403	60	-0.5763771015205776
2403	61	-0.48885940704923314
2404	1	-0.058213031367586154
2404	2	0.05221101859786614
2404	3	-0.07103465658084218
2404	4	0.07451541900859837
2404	5	-0.01221031417755114
2404	6	-1.0942791251977724E-4
2404	7	0.08498315568742498
2404	8	0.023701982346999273
2404	9	0.076089285200716
2404	10	0.05061363759825355
2404	11	0.013682621346575274
2404	12	-0.0099031218414396
2404	13	0.022858861229003935
2404	14	-0.0228169560975644
2404	15	0.03443209022687199
2404	16	0.04026353423419238
2404	17	-0.043822681276047604
2404	18	-0.09889516995280445
2404	19	0.031498576597243755
2404	20	-0.07793449285845355
2404	21	-0.07110794350293759
2404	22	-0.007712454469256613
2404	23	0.04138892132161942
2404	24	0.08809998189387613
2404	25	0.017849103942864986
2404	26	0.03787436664369892
2404	27	-0.006371895098162383
2404	28	-0.05908587124474707
2404	29	0.014173927086687854
2404	30	-0.013008426931925499
2404	31	0.04970111463282478
2404	32	-0.03092051568974341
2404	33	-0.032451304366858835
2404	34	0.020371338193463373
2404	35	0.0017492754606323047
2404	36	-0.01623276295271836
2404	37	-0.09869694743764512
2404	38	-0.026195413488636632
2404	39	-0.0475872248735314
2404	40	0.036469444615185864
2404	41	0.08771277891698048
2404	42	-0.11323123229676213
2404	43	-0.060663014314506825
2404	44	0.11212745085404663
2404	45	-0.06095850759824373
2404	46	-0.06574067517223625
2404	47	-0.275294773436336
2404	48	-0.2555947807513448
2404	49	-0.24639004841573353
2404	50	-0.014164629786350247
2404	51	-0.16668510121490954
2404	52	-0.1455006725434913
2404	53	-0.004334100849149536
2404	54	-0.14908726287048069
2404	55	0.06553761768316946
2404	56	-0.3361964857611781
2404	57	-0.5079868223751092
2404	58	-0.17058462467266367
2404	59	-0.31997915135511334
2404	60	0.08479003514272421
2404	61	-0.1812211330535618
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	9.0
14	34.0
15	140.0
16	398.0
17	787.0
18	1081.0
19	1264.0
20	1318.0
21	1332.0
22	1489.0
23	1750.0
24	2238.0
25	2607.0
26	3087.0
27	3653.0
28	5483.0
29	7166.0
30	8856.0
31	9562.0
32	11164.0
33	13569.0
34	18852.0
35	28800.0
36	45452.0
37	69763.0
38	143725.0
39	461234.0
40	359584.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	35.78391932678217	19.912568033777138	16.811929938183194	27.49158270125751
2	26.48478865357519	23.097035279895252	19.869694129095304	30.548481937434254
3	24.19675572091262	19.938276166413566	23.157646523530033	32.70732158914378
4	25.498818080749118	19.33565095230227	21.673999520091797	33.49153144685681
5	28.23587239091429	22.971163162976993	21.06788708374398	27.725077362364736
6	27.175673801910833	22.032436148545703	22.502380859467436	28.28950919007603
7	27.426670773839522	22.61305865092656	22.61546649485178	27.344804080382133
8	25.866969114004768	24.550459690616965	24.192853353171753	25.389717842206515
9	25.633823398763035	24.60085835484479	24.257366964547405	25.507951281844775
10	28.00081700635256	23.23436541273351	23.059423097201336	25.705394483712595
11	26.236448612874323	24.717680299768265	23.57843800673698	25.467433080620427
12	27.009615600171706	23.165036113507423	23.56515335059785	26.260194935723018
13	28.146699136580377	23.276710254176987	21.875096002397882	26.701494606844754
14	28.170611517630817	24.088402744277843	22.374931189632655	25.36605454845869
15	26.68879115441171	23.264172859945685	23.87177982010915	26.175256165533455
16	27.98064093484125	22.808426125272646	21.91536511631962	27.29556782356648
17	28.630924852851674	23.766914065710893	22.094957061500487	25.507204019936946
18	27.048805335782138	23.164537938902203	23.45306406442394	26.333592660891714
19	28.941370661002974	22.80427467022917	21.823036756152664	26.431317912615192
20	27.28892549549692	23.509191736611765	23.503296670450027	25.698586097441297
21	26.942611115769964	23.937123722493496	22.939030900940473	26.181234260796067
22	28.996418954879495	23.094876523272642	21.852927232465706	26.055777289382153
23	28.39578643918907	23.771895811763063	22.31473509150222	25.517582657545645
24	27.735622058175167	23.094046232263945	22.977639432844818	26.192692276716066
25	28.07238809130212	24.057681976956104	21.696749493730056	26.173180438011716
26	27.585256356500388	24.847039638923047	22.211945064625702	25.355758939950864
27	27.156743166912573	24.080265892392624	22.784679802423952	25.978311138270854
28	28.1024446258169	24.043982175312625	21.761927337912663	26.091645860957808
29	27.722005285632562	24.92193188790739	22.16495059353353	25.191112232926514
30	27.312588789244742	24.521316476211748	22.528368968039608	25.6377257665039
31	28.463289098196025	23.705721618370024	22.473569761465694	25.357419521968254
32	28.58650428388646	24.605757071796095	22.15631556704309	24.651423077274355
33	27.686569704638618	24.35035524236771	23.039150971986626	24.92392408100705
34	28.884329668705583	24.21610150141523	21.276373156027457	25.62319567385173
35	28.291003713891683	24.970254824613477	21.705218462018752	25.033522999476087
36	27.861992349698646	24.78202785294218	22.067806545516138	25.288173251843038
37	29.16687769896471	24.355756449077838	21.062075046683113	25.415290805274342
38	29.38682178716818	25.260358503051734	21.360481635208327	23.992338074571755
39	28.63964290844298	24.77588369947783	21.581256014420493	25.003217377658693
40	29.731724672180356	24.59106092094218	20.483777359126602	25.193437047750866
41	29.40857541159601	25.797141640173464	20.871025085582247	23.923257862648278
42	29.195190622361235	26.032113995634333	21.238677944232673	23.534017437771766
43	30.397285944750774	24.94883331658913	20.87567471523094	23.778206023429153
44	30.099709647234256	25.659313332729987	21.34379278593354	22.897184234102212
45	30.247584475882956	25.183971730251738	21.502378368594407	23.066065425270903
46	31.21885889785511	24.740181186103918	19.740168731738787	24.300791184302188
47	31.270752085898586	25.269408675046517	20.011424804279653	23.448414434775245
48	30.728821144522943	26.152838308298676	20.340386101924864	22.777954445253517
49	32.22002379614031	25.013097840662173	19.284172909763143	23.482705453434374
50	31.667465129853362	26.23935463140476	19.719494485622267	22.37368575311961
51	31.40733495682902	26.11589035841172	20.08000684159791	22.39676784316135
52	32.256639629623784	26.01891236859607	19.346942910020534	22.377505091759613
53	32.31343153461857	25.538755078267382	19.023959707637932	23.12385367947612
54	31.692622947416837	25.20348356895608	19.18046956277706	23.923423920850016
55	33.15161030789682	24.99117815803261	19.37035711646575	22.486854417604825
56	31.853533344902058	26.232961390637804	20.690934965796163	21.222570298663978
57	32.531133837098565	26.79523446172649	19.813151311403136	20.86048038977181
58	31.769424865721184	27.177583471230832	19.938774341018785	21.114217322029198
59	31.530051967914236	28.090322377089944	19.196411150144012	21.183214504851804
60	30.939964148034242	26.113399485385635	21.811578740232665	21.135057626347457
61	31.80902974683597	27.36248927886735	18.964095725910973	21.864385248385705
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	3.5
3	5.0
4	6.0
5	7.0
6	6.0
7	7.5
8	10.0
9	12.5
10	15.0
11	20.5
12	23.5
13	21.0
14	35.0
15	49.0
16	44.0
17	82.0
18	125.0
19	168.0
20	256.5
21	302.0
22	407.0
23	512.0
24	692.0
25	1177.5
26	1483.0
27	1955.0
28	2427.0
29	3123.5
30	4529.0
31	5238.0
32	6147.0
33	7056.0
34	8347.5
35	11398.0
36	13157.0
37	14984.5
38	16812.0
39	19322.5
40	25316.5
41	28800.0
42	32144.5
43	38978.5
44	42468.0
45	46641.0
46	50814.0
47	54555.5
48	62407.5
49	66518.0
50	69665.5
51	72813.0
52	77651.5
53	86193.5
54	89897.0
55	91716.0
56	93535.0
57	91767.0
58	86937.5
59	83876.0
60	80257.0
61	71786.5
62	66935.0
63	60231.5
64	53528.0
65	47350.5
66	35471.0
67	29769.0
68	25516.5
69	21264.0
70	17551.0
71	11410.5
72	8983.0
73	7277.5
74	5572.0
75	4465.0
76	2610.0
77	1862.0
78	1434.0
79	1006.0
80	735.5
81	349.0
82	233.0
83	188.0
84	104.0
85	65.0
86	57.0
87	49.0
88	50.0
89	49.0
90	47.0
91	52.5
92	58.0
93	57.5
94	59.5
95	62.0
96	71.5
97	81.0
98	89.0
99	248.0
100	399.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002656931227826041
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	6.642328069565102E-4
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1204397.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.09036328656592
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.42635888047342	70.94771198033185
2	10.288654983642259	16.891988506746596
3	1.927813000541526	4.747646086890557
4	0.56572408881062	1.8576198388170109
5	0.25447979221963296	1.044516929619974
6	0.1566116609168304	0.771378488374505
7	0.10116676274780456	0.581337141454517
8	0.07224406565306503	0.4744433275806901
9	0.04905206196926281	0.3624031428310735
>10	0.15410041390505258	1.8392822336779555
>50	0.002277204814775585	0.128162310010974
>100	0.0014087211410401658	0.2567261951271454
>500	0.0	0.0
>1k	1.0836316469539737E-4	0.09678381853719133
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.8141833631269424	0.0	0.0	8.302910086956377E-5	0.0
2	0.8156778869425945	0.0	0.0	8.302910086956377E-5	0.0
3	0.8164251488504206	0.0	0.0	8.302910086956377E-5	0.0
4	0.8179196726660727	0.0	0.0	8.302910086956377E-5	0.0
5	0.8189160218765075	0.0	0.0	8.302910086956377E-5	0.0
6	0.8220711277095509	0.0	0.0	8.302910086956377E-5	0.0
7	0.8258074372486813	0.0	0.0	8.302910086956377E-5	0.0
8	0.8336121727304203	0.0	0.0	8.302910086956377E-5	0.0
9	0.8374315113704203	0.0	0.0	8.302910086956377E-5	0.0
10	0.8427453738260723	0.0	0.0	8.302910086956377E-5	0.0
11	0.8505501093078113	0.0	0.0	8.302910086956377E-5	0.0
12	0.8640838527495502	0.0	0.0	8.302910086956377E-5	0.0
13	0.882018138537376	0.0	0.0	8.302910086956377E-5	8.302910086956377E-5
14	0.902360268250419	0.0	0.0	8.302910086956377E-5	8.302910086956377E-5
15	0.9330810355721577	0.0	0.0	8.302910086956377E-5	8.302910086956377E-5
16	0.9734331785947656	0.0	0.0	8.302910086956377E-5	8.302910086956377E-5
17	1.0157780200382431	0.0	0.0	8.302910086956377E-5	8.302910086956377E-5
18	1.0785480202956335	8.302910086956377E-5	0.0	8.302910086956377E-5	8.302910086956377E-5
19	1.1644001105947623	8.302910086956377E-5	0.0	8.302910086956377E-5	8.302910086956377E-5
20	1.2723379417251952	8.302910086956377E-5	0.0	8.302910086956377E-5	1.6605820173912753E-4
21	1.414981937019106	8.302910086956377E-5	0.0	8.302910086956377E-5	1.6605820173912753E-4
22	1.600302890159972	8.302910086956377E-5	0.0	8.302910086956377E-5	1.6605820173912753E-4
23	1.8227378513895336	8.302910086956377E-5	0.0	8.302910086956377E-5	1.6605820173912753E-4
24	2.1047877070434415	8.302910086956377E-5	0.0	8.302910086956377E-5	2.490873026086913E-4
25	2.465466121220827	8.302910086956377E-5	0.0	8.302910086956377E-5	2.490873026086913E-4
26	2.886091546226037	8.302910086956377E-5	0.0	8.302910086956377E-5	2.490873026086913E-4
27	3.3733893392295067	8.302910086956377E-5	0.0	8.302910086956377E-5	2.490873026086913E-4
28	3.9481998045494966	8.302910086956377E-5	0.0	8.302910086956377E-5	3.3211640347825507E-4
29	4.5937510638103545	8.302910086956377E-5	0.0	8.302910086956377E-5	3.3211640347825507E-4
30	5.323410802252082	8.302910086956377E-5	0.0	8.302910086956377E-5	3.3211640347825507E-4
31	6.1610083718242405	8.302910086956377E-5	0.0	8.302910086956377E-5	3.3211640347825507E-4
32	7.056892370206834	8.302910086956377E-5	0.0	8.302910086956377E-5	3.3211640347825507E-4
33	8.047927718185948	8.302910086956377E-5	0.0	8.302910086956377E-5	3.3211640347825507E-4
34	9.163091571965058	8.302910086956377E-5	0.0	8.302910086956377E-5	3.3211640347825507E-4
35	10.291872198286777	8.302910086956377E-5	0.0	8.302910086956377E-5	3.3211640347825507E-4
36	11.508580642429365	8.302910086956377E-5	0.0	8.302910086956377E-5	3.3211640347825507E-4
37	12.762403094660648	8.302910086956377E-5	0.0	8.302910086956377E-5	3.3211640347825507E-4
38	14.065046658203233	1.6605820173912753E-4	0.0	8.302910086956377E-5	3.3211640347825507E-4
39	15.472389917942339	1.6605820173912753E-4	0.0	8.302910086956377E-5	3.3211640347825507E-4
40	16.972559712453616	1.6605820173912753E-4	0.0	8.302910086956377E-5	3.3211640347825507E-4
41	18.509428369549244	1.6605820173912753E-4	0.0	8.302910086956377E-5	3.3211640347825507E-4
42	20.11886446080487	1.6605820173912753E-4	0.0	8.302910086956377E-5	3.3211640347825507E-4
43	21.82361795985875	1.6605820173912753E-4	0.0	8.302910086956377E-5	3.3211640347825507E-4
44	23.524635149373506	1.6605820173912753E-4	0.0	8.302910086956377E-5	3.3211640347825507E-4
45	25.182062060931734	1.6605820173912753E-4	0.0	8.302910086956377E-5	3.3211640347825507E-4
46	26.887230705489966	1.6605820173912753E-4	0.0	8.302910086956377E-5	3.3211640347825507E-4
47	28.516510751853417	1.6605820173912753E-4	0.0	8.302910086956377E-5	3.3211640347825507E-4
48	30.163475996702083	1.6605820173912753E-4	0.0	8.302910086956377E-5	3.3211640347825507E-4
49	31.88458622862727	1.6605820173912753E-4	0.0	8.302910086956377E-5	3.3211640347825507E-4
50	33.5611098333855	1.6605820173912753E-4	0.0	8.302910086956377E-5	3.3211640347825507E-4
>>END_MODULE