##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-29C_S28_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1204397
Sequences flagged as poor quality	0
Sequence length	61
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.4823069137502	40.0	40.0	40.0	40.0	40.0
2	39.23599444369257	40.0	40.0	40.0	40.0	40.0
3	39.169994611411354	40.0	40.0	40.0	40.0	40.0
4	39.03324236111515	40.0	40.0	40.0	40.0	40.0
5	39.07351147503689	40.0	40.0	40.0	40.0	40.0
6	39.20125340730673	40.0	40.0	40.0	40.0	40.0
7	39.226620458204394	40.0	40.0	40.0	40.0	40.0
8	39.212339452854835	40.0	40.0	40.0	40.0	40.0
9	39.23303196537354	40.0	40.0	40.0	40.0	40.0
10	39.24217014821525	40.0	40.0	40.0	40.0	40.0
11	39.23168523335744	40.0	40.0	40.0	40.0	40.0
12	39.247344521781436	40.0	40.0	40.0	40.0	40.0
13	39.08828733382763	40.0	40.0	40.0	40.0	40.0
14	39.242369418057336	40.0	40.0	40.0	40.0	40.0
15	39.14529179332064	40.0	40.0	40.0	40.0	40.0
16	39.33315011578408	40.0	40.0	40.0	40.0	40.0
17	39.284923492835006	40.0	40.0	40.0	40.0	40.0
18	39.370875218055176	40.0	40.0	40.0	40.0	40.0
19	39.323110236906935	40.0	40.0	40.0	40.0	40.0
20	39.31555790989184	40.0	40.0	40.0	40.0	40.0
21	39.31083853579841	40.0	40.0	40.0	40.0	40.0
22	39.37389747732683	40.0	40.0	40.0	40.0	40.0
23	39.360606178859626	40.0	40.0	40.0	40.0	40.0
24	39.36377789051284	40.0	40.0	40.0	40.0	40.0
25	39.327547312057405	40.0	40.0	40.0	40.0	40.0
26	39.286972651044465	40.0	40.0	40.0	40.0	40.0
27	39.30753065641977	40.0	40.0	40.0	40.0	40.0
28	39.315800354866376	40.0	40.0	40.0	40.0	40.0
29	39.32041677287472	40.0	40.0	40.0	40.0	40.0
30	39.31623874851897	40.0	40.0	40.0	40.0	40.0
31	39.26990518906972	40.0	40.0	40.0	40.0	40.0
32	39.2866704251173	40.0	40.0	40.0	40.0	40.0
33	39.28648111876732	40.0	40.0	40.0	40.0	40.0
34	39.296192202405024	40.0	40.0	40.0	40.0	40.0
35	39.289363889149506	40.0	40.0	40.0	40.0	40.0
36	39.255292067316674	40.0	40.0	40.0	40.0	40.0
37	39.27381752030269	40.0	40.0	40.0	40.0	40.0
38	39.25349863873789	40.0	40.0	40.0	40.0	40.0
39	38.015831988953806	40.0	40.0	40.0	40.0	40.0
40	39.253638127627354	40.0	40.0	40.0	40.0	40.0
41	39.251333239787215	40.0	40.0	40.0	40.0	40.0
42	39.22585326931236	40.0	40.0	40.0	40.0	40.0
43	39.241100733396046	40.0	40.0	40.0	40.0	40.0
44	39.19448487500384	40.0	40.0	40.0	40.0	40.0
45	39.064796740609616	40.0	40.0	40.0	40.0	40.0
46	39.061429080278344	40.0	40.0	40.0	40.0	40.0
47	39.143441904953264	40.0	40.0	40.0	40.0	40.0
48	39.221834660830275	40.0	40.0	40.0	40.0	40.0
49	39.20913452956127	40.0	40.0	40.0	40.0	40.0
50	39.18713513899487	40.0	40.0	40.0	40.0	40.0
51	39.14007756578603	40.0	40.0	40.0	40.0	40.0
52	39.14773284888621	40.0	40.0	40.0	40.0	40.0
53	39.0786592792908	40.0	40.0	40.0	40.0	40.0
54	38.99032627945769	40.0	40.0	40.0	40.0	40.0
55	39.03624137223856	40.0	40.0	40.0	40.0	40.0
56	39.03580297858596	40.0	40.0	40.0	40.0	40.0
57	38.96473671056969	40.0	40.0	40.0	40.0	40.0
58	38.94055531523244	40.0	40.0	40.0	40.0	40.0
59	38.874098822896435	40.0	40.0	40.0	40.0	40.0
60	38.83102332536531	40.0	40.0	40.0	40.0	40.0
61	38.76249110550757	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.09456894702599072
1101	2	-0.06303024857952977
1101	3	0.07056690399996768
1101	4	-0.05927622684173883
1101	5	-0.0851644971393597
1101	6	-0.05174119147037004
1101	7	-0.01518905084662947
1101	8	-0.023753076491757952
1101	9	0.02925611884483459
1101	10	0.05326195825058733
1101	11	0.018956066056425414
1101	12	0.03150908864061108
1101	13	-0.01971042090303854
1101	14	0.023331700722373228
1101	15	-0.00337953123321455
1101	16	0.07705516472859131
1101	17	-0.026006910571240383
1101	18	0.11447692193446812
1101	19	-0.0255101245365168
1101	20	0.06257323660172176
1101	21	-0.02729186039123732
1101	22	0.019159415352710596
1101	23	0.05170122736842586
1101	24	0.09209970955338775
1101	25	-0.014375519105300327
1101	26	0.05746825673268319
1101	27	0.13815125083840485
1101	28	0.054094414050936734
1101	29	0.10804832894044836
1101	30	-0.01475961631377487
1101	31	0.08121893810697145
1101	32	0.12547847269654966
1101	33	-0.03033573958572333
1101	34	0.10410698971728038
1101	35	0.06500867190417381
1101	36	-0.13419117422682092
1101	37	-0.060215886331981494
1101	38	-0.05943403314388718
1101	39	1.2463024767333124
1101	40	-0.07733052014287267
1101	41	-0.05137720861967665
1101	42	-0.0116951878828786
1101	43	-0.008339016764381313
1101	44	-0.045476412583525416
1101	45	0.10953042316087647
1101	46	0.01692763309513623
1101	47	-0.01575658012798442
1101	48	0.010323496988029035
1101	49	0.02278105933464758
1101	50	-0.11890277400616611
1101	51	0.09002963694505439
1101	52	0.034535036270845865
1101	53	-0.029680905185365702
1101	54	0.0419146758753115
1101	55	-0.008359971421512569
1101	56	-0.04977775812612606
1101	57	0.05468680978052731
1101	58	-0.01902606607286117
1101	59	-0.024823354279128296
1101	60	-0.02555875646458361
1101	61	0.057720600975954994
1102	1	-0.021428042466723696
1102	2	-0.02530268665592672
1102	3	0.04814749172498267
1102	4	-0.10116658221327413
1102	5	-0.13322134707120625
1102	6	-0.12965139093535072
1102	7	-0.14965901283787986
1102	8	-0.10990191776345881
1102	9	-0.13915466895299033
1102	10	-0.09561948260660103
1102	11	-0.10214998647135332
1102	12	-0.03204645017779484
1102	13	-0.09348768951910102
1102	14	-0.023769281770348982
1102	15	-0.16661510419540804
1102	16	-0.05643805243130373
1102	17	-0.04021172968118947
1102	18	-0.07351712404468458
1102	19	-0.04691975813167204
1102	20	-0.1582929241185198
1102	21	-0.09906701351964387
1102	22	-0.049562353094550815
1102	23	-0.028331859334194576
1102	24	-0.024953750444929312
1102	25	-0.21877073871102226
1102	26	-0.1150707050398907
1102	27	-0.13824058081581825
1102	28	-0.14929261249968562
1102	29	-0.07273508859616129
1102	30	-0.046455197508002755
1102	31	-0.1098247927623035
1102	32	-0.1766039150428682
1102	33	-0.04659288563298958
1102	34	-0.12704511860203382
1102	35	-0.0951954508522519
1102	36	-0.16171920509894022
1102	37	-0.14945220321644115
1102	38	-0.042785502060027625
1102	39	1.0871464519209297
1102	40	-0.02777419776158041
1102	41	-0.12210690651191669
1102	42	-0.028968455108874025
1102	43	0.05867979072690588
1102	44	-0.10282872320212277
1102	45	-0.07427598791630174
1102	46	0.014085400773360846
1102	47	-0.07104889995642338
1102	48	-0.03779783501602196
1102	49	-0.055147374814687566
1102	50	-0.0744582414714614
1102	51	-0.1020096019815071
1102	52	-0.08553348914440306
1102	53	-0.0880316308399145
1102	54	-0.13259759586905062
1102	55	-0.013246866827756776
1102	56	-0.05979626368802826
1102	57	-0.22879170731680176
1102	58	-0.0958479984340741
1102	59	-0.14686598247853766
1102	60	-0.005722062597847355
1102	61	-0.05300357365682373
1103	1	0.022938430340865068
1103	2	0.03255995074066931
1103	3	-0.030188083658444498
1103	4	0.014396794431803528
1103	5	0.013297274505632117
1103	6	-0.015429456088988047
1103	7	0.08466104185657741
1103	8	0.08737137448522958
1103	9	0.03742437133791299
1103	10	0.008743413288705426
1103	11	0.06398941461316099
1103	12	0.026279152120203264
1103	13	-0.045945460919313064
1103	14	-0.024455155698348108
1103	15	-0.012574997717472058
1103	16	0.02707028841374637
1103	17	0.12585031120447354
1103	18	0.016248620101606548
1103	19	-6.814875229039785E-4
1103	20	0.0030892235002895063
1103	21	0.10494857894683207
1103	22	0.01556935164709472
1103	23	-0.053186016418067084
1103	24	-0.0042371677080979
1103	25	0.09318625699369676
1103	26	0.04532171055026879
1103	27	-0.15894360557965115
1103	28	-0.015800810387872843
1103	29	0.11706058278169706
1103	30	-0.05599761791322777
1103	31	0.07425659176195154
1103	32	-0.03219953972547529
1103	33	0.05316588213867135
1103	34	-0.015237300476655946
1103	35	0.09426844895114073
1103	36	0.0043053519790348105
1103	37	0.09546699624905841
1103	38	0.007303232879408483
1103	39	1.2988884939390815
1103	40	-0.051197589930538356
1103	41	0.02046474986764224
1103	42	-0.030873749078629942
1103	43	-0.03153894922299827
1103	44	0.01261263168599669
1103	45	0.0261032565018553
1103	46	-0.0021155794282066154
1103	47	-0.13360618488460574
1103	48	0.03787000979862398
1103	49	-4.8003264195983775E-4
1103	50	0.0875946270467054
1103	51	0.01714823256297393
1103	52	-0.0778796146246421
1103	53	0.10840585225000154
1103	54	-0.022831448971203372
1103	55	0.0033656377306101604
1103	56	0.006231064799123942
1103	57	-0.013235887247851963
1103	58	-0.08134176485691569
1103	59	-0.003628390366571921
1103	60	-0.0015725253334579747
1103	61	0.03212933136418883
1104	1	-0.048384867110762286
1104	2	-0.025378622505002113
1104	3	0.027879182379749068
1104	4	0.030291095470253993
1104	5	0.10020250661554542
1104	6	0.04198639533241533
1104	7	-0.024679319316042836
1104	8	-0.1064348502840744
1104	9	0.039389395736314725
1104	10	0.033324410443583474
1104	11	0.00947972943615838
1104	12	0.013614164254384775
1104	13	0.006215142834506082
1104	14	-0.025121663082416035
1104	15	0.05075802702267396
1104	16	0.013988362851549141
1104	17	0.008930416201621938
1104	18	-0.06134087091233198
1104	19	0.003633696353489313
1104	20	0.052227231090604676
1104	21	-0.050634673633460636
1104	22	0.06830294153051852
1104	23	0.002002034745878234
1104	24	0.037816402721823295
1104	25	-0.09574769907799663
1104	26	0.05802928158094289
1104	27	-0.016379212355388972
1104	28	0.04463736768333604
1104	29	0.02213650950793067
1104	30	0.011564076805093748
1104	31	-0.002611141559093255
1104	32	-0.01393432665400951
1104	33	-0.008099972510166253
1104	34	-0.052884052371645396
1104	35	0.12400308710810748
1104	36	-0.022205363279340418
1104	37	-0.01869361747676379
1104	38	-0.006419596265210714
1104	39	1.1666664195002099
1104	40	-0.1333049391244856
1104	41	0.04108793831444757
1104	42	-0.09743393088179175
1104	43	-0.006267457964611367
1104	44	-0.008944960764281973
1104	45	-0.08069397338241657
1104	46	-0.08753444347377126
1104	47	0.025776941374616058
1104	48	0.06151822003224794
1104	49	0.06327505055489979
1104	50	0.013314225261069623
1104	51	-7.251075863621281E-4
1104	52	0.06937216089793452
1104	53	0.05010518734622593
1104	54	-0.03379742246210782
1104	55	0.044994724080510196
1104	56	0.0812968295112384
1104	57	0.09970382940969813
1104	58	-0.04728682206322077
1104	59	-0.06934418574260093
1104	60	0.021966531851013826
1104	61	0.10958190941772017
1201	1	0.03451020272733274
1201	2	0.02755795358404356
1201	3	0.15327432629245408
1201	4	-0.011352159539704587
1201	5	0.034656935502958675
1201	6	0.013026889547397502
1201	7	0.015188319535809569
1201	8	-0.06666180947616596
1201	9	-0.06358764276318851
1201	10	0.08264913535492013
1201	11	-0.05080803890083985
1201	12	0.07963055091899207
1201	13	-0.041679194412417075
1201	14	-0.01863082373408531
1201	15	0.002978947162624479
1201	16	0.07800405525350129
1201	17	0.013422735954975451
1201	18	-0.019286603711456962
1201	19	0.02589142002553757
1201	20	0.13608730971027683
1201	21	0.00805471396109425
1201	22	0.017646232667146933
1201	23	-0.013272831015967768
1201	24	0.031974701675025585
1201	25	0.012985141095072095
1201	26	0.09282287707589632
1201	27	0.06633970940088574
1201	28	0.004334251629202868
1201	29	0.040229744578901716
1201	30	0.07140114526607988
1201	31	-0.004418834065432975
1201	32	-9.691622653207332E-5
1201	33	-0.006765813890446282
1201	34	-0.06305008009375968
1201	35	-0.04249327589612051
1201	36	-0.04283806652989597
1201	37	-0.004859469050799703
1201	38	-0.01343724885824571
1201	39	1.1866940214696982
1201	40	0.06817693379718293
1201	41	0.06852843872722758
1201	42	0.06962349638305199
1201	43	0.16507515195736744
1201	44	0.1227132260576127
1201	45	-0.07339035198988597
1201	46	0.14820333835975674
1201	47	-0.03558565646164169
1201	48	0.06077883302213394
1201	49	-0.07834202689289782
1201	50	0.06464290643657478
1201	51	0.05157945570910982
1201	52	0.04978057979764827
1201	53	-0.0019318918250021966
1201	54	-0.04115106121120249
1201	55	0.08888441080060261
1201	56	0.07098183705325312
1201	57	0.08724678929007013
1201	58	0.08509281076045028
1201	59	0.043147567509812745
1201	60	0.03225651563071352
1201	61	0.16162116325661913
1202	1	0.09390933845411809
1202	2	0.010730862903059801
1202	3	-0.002923301476812412
1202	4	0.03265465372857079
1202	5	-0.0056063149107075105
1202	6	0.05694799287878283
1202	7	0.12659780332689508
1202	8	0.0603149095286426
1202	9	0.06814476866439634
1202	10	0.003579703454619221
1202	11	0.041249538294188426
1202	12	0.05702086067174861
1202	13	0.019813520713142907
1202	14	0.010684124146074225
1202	15	0.06616651583637179
1202	16	0.03583825918753547
1202	17	-0.04586355283296939
1202	18	0.07131282982377485
1202	19	-0.0030702094627628185
1202	20	0.07280543323764022
1202	21	0.04042378363615029
1202	22	0.06442719984870138
1202	23	0.04651482060027945
1202	24	0.053002385312929334
1202	25	-0.06302022773238747
1202	26	0.10162063937483623
1202	27	0.1278716522718497
1202	28	0.08078832138016168
1202	29	0.03387834270422019
1202	30	0.08292066825669764
1202	31	0.08210951180935666
1202	32	0.08731674075182383
1202	33	0.08987952873502536
1202	34	-0.011296055790559478
1202	35	0.02366942171532571
1202	36	-0.18317881860906482
1202	37	-0.19039112120655943
1202	38	-0.10101426521473655
1202	39	1.1193214434639103
1202	40	-0.1097192795297488
1202	41	-0.06825467926324791
1202	42	0.0013030040909640661
1202	43	-0.15085543696527992
1202	44	-0.11189287297014516
1202	45	-0.17212416903356598
1202	46	-0.06408862192725451
1202	47	-0.004940995485419819
1202	48	-0.04049269672962197
1202	49	-0.016740763759493404
1202	50	-0.06804924133756174
1202	51	-0.08443012338389622
1202	52	-0.023588469591835803
1202	53	-0.10866655683281579
1202	54	0.08136632292689683
1202	55	-0.046190419482300626
1202	56	-0.027919906982752707
1202	57	-0.005806527798675631
1202	58	-0.10442421911886157
1202	59	-0.05673809435964472
1202	60	0.03205339114079209
1202	61	-0.005170373921544069
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2403	3	-0.07123625526775612
2403	4	0.11483799538692807
2403	5	0.055366503442726867
2403	6	3.173316959106387E-4
2403	7	-0.04361293157968049
2403	8	-0.13998766907161553
2403	9	0.018733604384244984
2403	10	-0.10609511274627437
2403	11	-0.027686530130544895
2403	12	-0.04955498399340996
2403	13	-0.19262263822508174
2403	14	0.015294397693260464
2403	15	-0.0823519717078085
2403	16	0.02104980100265408
2403	17	0.05517730731424564
2403	18	0.010788255567469207
2403	19	-0.007267932424255719
2403	20	0.027700455922733624
2403	21	-0.044675911592449324
2403	22	0.0178698563203028
2403	23	-0.07688871619782844
2403	24	-0.08491153231350523
2403	25	-0.05751263590926925
2403	26	-0.027597619461090517
2403	27	-0.08785384359644866
2403	28	0.017639858992303914
2403	29	-0.04974851096324073
2403	30	0.04102700019570449
2403	31	-0.08540926826943007
2403	32	-0.04314110669839977
2403	33	-0.10021359881829994
2403	34	-0.08553938966414165
2403	35	-0.18850775174455237
2403	36	-0.12569483347832033
2403	37	-0.11246111798099889
2403	38	-0.08687576097069893
2403	39	1.283666821351325
2403	40	-0.1572525223980179
2403	41	-0.12767441482828445
2403	42	-0.16208003245704106
2403	43	-0.10305297094485155
2403	44	-0.04779728740667366
2403	45	-0.013796329803227536
2403	46	-0.16494880096617948
2403	47	-0.14769760952421507
2403	48	-0.07211078390466241
2403	49	0.05975734860022186
2403	50	-0.14037038277732705
2403	51	-0.16285208834075604
2403	52	-0.13517551724661558
2403	53	-0.04791177326242746
2403	54	-0.14891244132223846
2403	55	-0.06651469226677875
2403	56	-0.018084269377311557
2403	57	-0.23768762634811225
2403	58	-0.16980200415524394
2403	59	-0.13899642006141733
2403	60	-0.05505759195327897
2403	61	-0.16139395614033702
2404	1	-0.048812952615847394
2404	2	-0.08334227238847092
2404	3	-0.036172091062049105
2404	4	-0.026833692580282786
2404	5	0.09387438400127479
2404	6	-0.06557170615761976
2404	7	0.14539164971784402
2404	8	-0.02932654676216373
2404	9	0.0483088288340241
2404	10	0.11501387620497638
2404	11	0.04564324458949898
2404	12	0.05637824565705074
2404	13	0.05417422900563906
2404	14	-0.017857719097726488
2404	15	0.11030609988502249
2404	16	-0.007218615927264693
2404	17	0.06896145732611814
2404	18	-0.02388015254207687
2404	19	0.060369801904009535
2404	20	-0.029791531213156475
2404	21	0.056105815501368284
2404	22	0.0707735099484168
2404	23	0.016441828853913876
2404	24	-0.057485112591095344
2404	25	0.08863190834704682
2404	26	0.1412602491906867
2404	27	0.04292990942621344
2404	28	0.06917896649122213
2404	29	0.024086113908794005
2404	30	-0.06384314269792668
2404	31	0.05493385139551066
2404	32	0.029416015174092536
2404	33	0.05202338422621011
2404	34	-0.0011580389157970217
2404	35	0.03239813657084056
2404	36	0.0380646289259019
2404	37	0.08503423047041991
2404	38	0.016786803570873587
2404	39	1.2767181683111133
2404	40	0.11115918493193533
2404	41	0.07720248116148554
2404	42	0.05104390992359242
2404	43	0.03054198502184846
2404	44	0.01785499313420047
2404	45	0.11403201882394853
2404	46	0.07311357834760912
2404	47	0.09216453156703608
2404	48	0.09941321580838292
2404	49	0.0028117021747817716
2404	50	0.08131396126093904
2404	51	-0.04850265848413926
2404	52	-0.010860519476004526
2404	53	0.10236877037124259
2404	54	0.018716088195070313
2404	55	0.0369514449455437
2404	56	0.0900102411824335
2404	57	0.11944192044949631
2404	58	-0.05009710067483297
2404	59	0.008741593755082988
2404	60	0.1349825124433437
2404	61	0.05361359956599898
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	12.0
17	42.0
18	134.0
19	195.0
20	244.0
21	321.0
22	421.0
23	620.0
24	889.0
25	1199.0
26	1585.0
27	2030.0
28	2698.0
29	3312.0
30	4117.0
31	5189.0
32	7186.0
33	8712.0
34	11173.0
35	14246.0
36	20891.0
37	30247.0
38	78887.0
39	541828.0
40	468218.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	25.431730567246515	18.166933328462292	25.38274339773347	31.018592706557722
2	24.79841924809632	17.552724953155526	29.886443673670083	27.76241212507807
3	27.484504668318948	19.908086632707708	21.62670884551995	30.98069985345339
4	28.332684322528202	22.47606063449178	20.90141373650051	28.289841306479506
5	27.39910511235083	21.168020179392673	21.383231608846582	30.049643099409913
6	28.783034165644715	22.668356032105695	22.70289613806743	25.845713664182156
7	27.458969094077784	23.755040904286542	22.567143558145695	26.21884644348998
8	25.162550222227388	24.97382507595087	23.36148296616481	26.50214173565693
9	25.438477045755715	24.30338526531141	23.29756990225806	26.960567786674815
10	25.863647949969987	23.414040387015245	23.651337557300458	27.070974105714313
11	25.29523072541695	24.160472003832623	23.408643495458723	27.1356537752917
12	27.2922466595317	23.164205822498726	22.316478702620483	27.227068815349092
13	25.675005832794334	23.07096414222221	22.957712448636123	28.296317576347334
14	25.42309554075608	23.507697212796113	23.26051957950742	27.808687666940386
15	26.80553007023432	22.435708491469175	22.236438649382222	28.522322788914284
16	26.320557092055196	23.755123933387413	22.53401494689874	27.39030402765865
17	25.664876282488247	22.547216573937	23.11438836197699	28.673518781597764
18	27.301628947929963	22.555187367620476	22.44866103120483	27.694522653244736
19	25.97158578110042	23.155155650503943	23.70771431679089	27.165544251604747
20	25.64793834591086	23.06897144380134	23.0649860469596	28.218104163328206
21	26.525805029404758	22.771063029881343	22.947748956531775	27.755382984182127
22	26.891880335138662	23.134481404387426	22.647017553182213	27.3266207072917
23	25.77704859776303	23.998482228036107	22.997649446154384	27.22681972804648
24	26.870790943517793	23.723988020561325	22.043562048062228	27.361658987858657
25	26.167368400950846	23.13315293877351	22.726808519117867	27.972670141157774
26	25.935883267726506	23.037088269067425	23.787837399130023	27.239191064076046
27	26.88880825840649	23.212196642801334	22.127338410839613	27.77165668795256
28	26.44177957932476	23.67574811295611	22.474150965171784	27.40832134254735
29	26.10210752766737	24.3842354306761	22.86056840061873	26.653088641037797
30	27.837332706740387	23.536010136192633	21.9778860292744	26.648771127792582
31	26.57678489733867	24.186377083303928	23.109489645025686	26.12734837433172
32	26.299052554929975	24.080099834190886	22.53011257915787	27.09073503172127
33	27.196098960724747	24.751888289326526	21.809253925408317	26.242758824540413
34	26.997576380545617	24.537092005376966	22.020562987121355	26.444768626956062
35	26.87137214722388	24.501389492003053	22.00852376749527	26.618714593277797
36	28.37104376712994	24.69949692667783	21.15755851268311	25.77190079350912
37	27.11597587838562	25.13398821152826	22.38912916588135	25.360906744204776
38	26.82396253062736	25.16171993121869	21.59180071023093	26.42251682792302
39	28.222210412084287	25.12647800542771	21.26870597271058	25.38260560977742
40	27.52672084038735	25.75446468232651	21.596616398081363	25.122198079204782
41	27.290918193917786	25.707885356738686	20.96368556215268	26.03751088719085
42	27.965114492978643	26.597791259858667	20.076104473857043	25.360989773305647
43	27.671689650505606	27.096464039681265	20.30252483192834	24.929321477884784
44	27.386152572615174	26.518000293923016	20.966757638884854	25.129089494576956
45	28.16978122662212	26.187212356058676	19.504781230773574	26.138225186545633
46	27.60974994125691	26.785686115126488	20.523382240241382	25.081181703375215
47	28.293494586917767	26.65366984474388	19.851676814206613	25.201158754131736
48	28.960301296001234	26.748655136138666	19.054431387657058	25.23661218020304
49	28.65981897995428	27.188626341646483	19.13455446999619	25.017000208403044
50	28.291750975799506	27.161475825662134	19.208699457072708	25.338073741465646
51	28.829115316627323	27.131668378449962	18.585732113248373	25.45348419167434
52	28.40691233870559	27.51551191176996	18.85084403232489	25.226731717199563
53	27.92633990287256	27.523067559949087	18.94948260415793	25.60110993302042
54	28.39371071166733	27.35136337935083	18.415854572869243	25.839071336112596
55	28.031620802775166	27.50853746729691	18.6200231319075	25.83981859802042
56	27.373532149283005	27.678331978575173	18.716419918017067	26.231715954124763
57	27.848375577156038	27.260529542999524	18.380152059495334	26.510942820349104
58	27.19194750568127	27.549221726723	18.602753078926636	26.6560776886691
59	26.859166869396056	27.51517979536648	18.739585037159674	26.88606829807779
60	27.337829635909088	27.008038047255184	18.366120141448373	27.28801217538735
61	27.066407505166485	27.270991209709088	18.661703740544024	27.0008975445804
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	3.5
2	2.5
3	2.0
4	3.5
5	5.0
6	5.0
7	6.0
8	7.0
9	9.5
10	12.0
11	11.5
12	18.0
13	25.0
14	35.0
15	45.0
16	47.0
17	79.5
18	110.0
19	157.0
20	264.5
21	325.0
22	413.0
23	501.0
24	677.5
25	1203.5
26	1553.0
27	1934.5
28	2316.0
29	3080.5
30	4569.5
31	5294.0
32	6414.5
33	7535.0
34	8773.5
35	11899.5
36	13787.0
37	15755.5
38	17724.0
39	20399.0
40	26438.5
41	29803.0
42	33459.0
43	40452.0
44	43789.0
45	47845.5
46	51902.0
47	55727.0
48	63480.0
49	67408.0
50	71597.0
51	75786.0
52	79767.5
53	86608.5
54	89468.0
55	91042.5
56	92617.0
57	91359.0
58	87109.0
59	84117.0
60	80090.5
61	73657.5
62	71251.0
63	60284.5
64	49318.0
65	43336.5
66	32163.0
67	26971.0
68	22854.0
69	18737.0
70	15640.5
71	10328.5
72	8113.0
73	6603.5
74	5094.0
75	4008.0
76	2282.5
77	1643.0
78	1241.0
79	839.0
80	626.0
81	298.5
82	184.0
83	144.5
84	73.5
85	42.0
86	34.5
87	27.0
88	24.0
89	15.0
90	9.0
91	7.5
92	6.0
93	5.0
94	6.5
95	9.0
96	7.0
97	5.0
98	5.5
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03429101865912984
3	1.6605820173912756E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	8.302910086956378E-5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.1377527509616843
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1204397.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.13197588901559
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.55160586626131	66.11603660897524
2	12.433254860525546	19.677360476901868
3	2.4219258796077123	5.749553409303011
4	0.6900429957926207	2.18417862821783
5	0.3166948918751495	1.2530346274019364
6	0.17673105428887623	0.8391046516096593
7	0.10973049719256066	0.6078233740691983
8	0.08525978983874784	0.5397420507057864
9	0.05723322311487607	0.407608022841932
>10	0.1561541557180548	1.7652636430535966
>50	9.111905230376392E-4	0.04930131956890593
>100	3.4169644613911475E-4	0.04037841163961582
>500	0.0	0.0
>1k	0.0	0.0
>5k	1.138988153797049E-4	0.7706147757114181
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCTTCAATCTGGGGGGGGGGGGGGG	8550	0.7098988124347703	TruSeq Adapter, Index 13 (97% over 37bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.814432450429551	0.0	0.0	0.0	0.0
2	0.8160930324469423	0.0	0.0	0.0	8.302910086956377E-5
3	0.8172554398591162	0.0	0.0	0.0	8.302910086956377E-5
4	0.818999050977377	0.0	0.0	0.0	8.302910086956377E-5
5	0.8206596329947683	0.0	0.0	0.0	8.302910086956377E-5
6	0.8252262335425944	8.302910086956377E-5	0.0	0.0	8.302910086956377E-5
7	0.8299588922921595	8.302910086956377E-5	0.0	0.0	8.302910086956377E-5
8	0.8619250961269416	8.302910086956377E-5	0.0	0.0	8.302910086956377E-5
9	0.867155929481724	8.302910086956377E-5	0.0	0.0	8.302910086956377E-5
10	0.8726358501391153	8.302910086956377E-5	0.0	0.0	8.302910086956377E-5
11	0.8786969745025934	8.302910086956377E-5	0.0	0.0	8.302910086956377E-5
12	0.8922307179443323	8.302910086956377E-5	0.0	0.0	1.6605820173912753E-4
13	0.9095007709252015	8.302910086956377E-5	0.0	0.0	1.6605820173912753E-4
14	0.9283483768225925	8.302910086956377E-5	0.0	0.0	1.6605820173912753E-4
15	0.9574915912278095	8.302910086956377E-5	0.0	0.0	1.6605820173912753E-4
16	0.996017094031287	8.302910086956377E-5	0.0	0.0	1.6605820173912753E-4
17	1.0367843825582428	8.302910086956377E-5	0.0	0.0	1.6605820173912753E-4
18	1.0951538404695462	8.302910086956377E-5	0.0	0.0	1.6605820173912753E-4
19	1.1812550180712837	8.302910086956377E-5	0.0	0.0	1.6605820173912753E-4
20	1.286286830671282	8.302910086956377E-5	0.0	0.0	1.6605820173912753E-4
21	1.4266890402417143	8.302910086956377E-5	0.0	0.0	1.6605820173912753E-4
22	1.613255429895624	8.302910086956377E-5	0.0	0.0	2.490873026086913E-4
23	1.833697692704316	8.302910086956377E-5	0.0	0.0	3.3211640347825507E-4
24	2.112509413424311	8.302910086956377E-5	0.0	0.0	3.3211640347825507E-4
25	2.474931438719957	8.302910086956377E-5	0.0	0.0	3.3211640347825507E-4
26	2.8950586891199497	8.302910086956377E-5	0.0	0.0	4.151455043478189E-4
27	3.3823564821234195	8.302910086956377E-5	0.0	0.0	4.151455043478189E-4
28	3.9387344870503664	8.302910086956377E-5	0.0	0.0	4.151455043478189E-4
29	4.5639436165981815	8.302910086956377E-5	0.0	0.0	4.151455043478189E-4
30	5.271849730612082	8.302910086956377E-5	0.0	0.0	4.981746052173826E-4
31	6.079058649265981	8.302910086956377E-5	0.0	0.0	4.981746052173826E-4
32	6.9426443274103145	8.302910086956377E-5	0.0	0.0	4.981746052173826E-4
33	7.904868577387688	8.302910086956377E-5	0.0	0.0	4.981746052173826E-4
34	8.983416597683322	8.302910086956377E-5	0.0	0.0	4.981746052173826E-4
35	10.076743797933737	8.302910086956377E-5	0.0	0.0	4.981746052173826E-4
36	11.255424913878064	8.302910086956377E-5	0.0	0.0	4.981746052173826E-4
37	12.472963649029348	8.302910086956377E-5	0.0	0.0	4.981746052173826E-4
38	13.743059805031065	8.302910086956377E-5	0.0	0.0	4.981746052173826E-4
39	15.108888514335389	8.302910086956377E-5	0.0	0.0	4.981746052173826E-4
40	16.615119433210147	8.302910086956377E-5	0.0	0.0	4.981746052173826E-4
41	18.157301952761422	8.302910086956377E-5	0.0	0.0	4.981746052173826E-4
42	19.774874895902265	8.302910086956377E-5	0.0	0.0	4.981746052173826E-4
43	21.48867856695093	8.302910086956377E-5	0.0	0.0	4.981746052173826E-4
44	23.20024045227612	8.302910086956377E-5	0.0	0.0	4.981746052173826E-4
45	24.882160948590872	8.302910086956377E-5	0.0	0.0	4.981746052173826E-4
46	26.607920810164753	8.302910086956377E-5	0.0	0.0	4.981746052173826E-4
47	28.256463607929945	8.302910086956377E-5	0.0	0.0	4.981746052173826E-4
48	29.917377741724696	8.302910086956377E-5	0.0	0.0	4.981746052173826E-4
49	31.64571150542554	8.302910086956377E-5	0.0	0.0	4.981746052173826E-4
50	33.32912652555594	8.302910086956377E-5	0.0	0.0	4.981746052173826E-4
>>END_MODULE