##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-29A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 613399 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.56618775055062 40.0 40.0 40.0 40.0 40.0 2 39.35294645084195 40.0 40.0 40.0 40.0 40.0 3 39.31748829065584 40.0 40.0 40.0 40.0 40.0 4 39.20982590450914 40.0 40.0 40.0 40.0 40.0 5 39.226721921620346 40.0 40.0 40.0 40.0 40.0 6 39.3450266466036 40.0 40.0 40.0 40.0 40.0 7 39.38457023894724 40.0 40.0 40.0 40.0 40.0 8 39.37967293719096 40.0 40.0 40.0 40.0 40.0 9 39.41047181361561 40.0 40.0 40.0 40.0 40.0 10-11 39.412460731106506 40.0 40.0 40.0 40.0 40.0 12-13 39.34046843897691 40.0 40.0 40.0 40.0 40.0 14-15 39.367445985402654 40.0 40.0 40.0 40.0 40.0 16-17 39.4741986863363 40.0 40.0 40.0 40.0 40.0 18-19 39.510175269278236 40.0 40.0 40.0 40.0 40.0 20-21 39.486031115146915 40.0 40.0 40.0 40.0 40.0 22-23 39.534146615824284 40.0 40.0 40.0 40.0 40.0 24-25 39.519715552193595 40.0 40.0 40.0 40.0 40.0 26-27 39.48945466164764 40.0 40.0 40.0 40.0 40.0 28-29 39.48464865446471 40.0 40.0 40.0 40.0 40.0 30-31 39.39433616102321 40.0 40.0 40.0 40.0 40.0 32-33 39.34420034205609 40.0 40.0 40.0 40.0 40.0 34-35 39.33335420828499 40.0 40.0 40.0 40.0 40.0 36-37 39.30637706167609 40.0 40.0 40.0 40.0 40.0 38-39 39.21608970218678 40.0 40.0 40.0 40.0 40.0 40-41 39.23619080265317 40.0 40.0 40.0 40.0 40.0 42-43 39.20593155919737 40.0 40.0 40.0 40.0 40.0 44-45 39.17443828280629 40.0 40.0 40.0 40.0 40.0 46-47 39.04365519391837 40.0 40.0 40.0 40.0 40.0 48-49 39.077528538680966 40.0 40.0 40.0 40.0 40.0 50-51 38.9382539229986 40.0 40.0 40.0 40.0 40.0 52-53 38.887390785021964 40.0 40.0 40.0 40.0 40.0 54-55 38.73825202191699 40.0 40.0 40.0 40.0 40.0 56-57 38.7440163944656 40.0 40.0 40.0 40.0 40.0 58-59 38.69671591549955 40.0 40.0 40.0 40.0 40.0 60-61 38.63136638225474 40.0 40.0 40.0 40.0 40.0 62-63 39.3921855399998 40.0 40.0 40.0 40.0 40.0 64-65 39.39079189184826 40.0 40.0 40.0 40.0 40.0 66-67 39.387808972431415 40.0 40.0 40.0 40.0 40.0 68-69 39.39753168759049 40.0 40.0 40.0 40.0 40.0 70-71 39.39401904632779 40.0 40.0 40.0 40.0 40.0 72-73 39.39522010443473 40.0 40.0 40.0 40.0 40.0 74-75 39.39656960135806 40.0 40.0 40.0 40.0 40.0 76-77 39.385189028096974 40.0 40.0 40.0 40.0 40.0 78-79 39.396340899904416 40.0 40.0 40.0 40.0 40.0 80-81 39.383479396819745 40.0 40.0 40.0 40.0 40.0 82-83 39.3921327758782 40.0 40.0 40.0 40.0 40.0 84-85 39.37753668371488 40.0 40.0 40.0 40.0 40.0 86-87 39.36197582872111 40.0 40.0 40.0 40.0 40.0 88-89 39.390575391489186 40.0 40.0 40.0 40.0 40.0 90-91 39.505471587982 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06590602291900893 1101 2 -0.14294023388866606 1101 3 -0.07640447184655841 1101 4 -0.17436318360353198 1101 5 0.021856833550536692 1101 6 -0.010906460707921894 1101 7 -0.05982542272099778 1101 8 -0.07370477697329392 1101 9 0.0070491676464499164 1101 10-11 -0.04485045778308461 1101 12-13 -0.19176119329109298 1101 14-15 -0.12081289614496171 1101 16-17 0.05295596796334934 1101 18-19 -0.030926213847720874 1101 20-21 -0.0025782316908902203 1101 22-23 -0.06332153492141401 1101 24-25 -0.046926685027891324 1101 26-27 -0.014901980169788942 1101 28-29 -0.03641248271378572 1101 30-31 -0.016433237758057828 1101 32-33 -0.03554946424607408 1101 34-35 -0.08623939569550743 1101 36-37 -0.19060243008564726 1101 38-39 0.06937068908739263 1101 40-41 -0.09797537037564297 1101 42-43 -0.11173226151166205 1101 44-45 -0.14888342490099404 1101 46-47 0.012635302059713638 1101 48-49 -0.18697123315799757 1101 50-51 -0.015804632531846607 1101 52-53 -0.14349498020857965 1101 54-55 -0.1194590692832378 1101 56-57 -7.268693617987765E-4 1101 58-59 -0.13320505138065641 1101 60-61 -0.16222222379938955 1101 62-63 0.23023018322459166 1101 64-65 -0.04187921379731563 1101 66-67 -0.05563533182571945 1101 68-69 0.013031242205570948 1101 70-71 -0.11329266773751101 1101 72-73 0.08086699150597099 1101 74-75 0.11035505885755015 1101 76-77 0.14845997794509458 1101 78-79 0.10081031207987223 1101 80-81 0.029288271600918847 1101 82-83 -0.0635865838287657 1101 84-85 -0.0104954885619577 1101 86-87 0.04006528163645129 1101 88-89 0.11172048642362142 1101 90-91 -0.5262397751933179 1102 1 -0.027775544832024934 1102 2 -0.003473931458252366 1102 3 0.030565043543028025 1102 4 -0.08887123264630503 1102 5 -0.08250868855617455 1102 6 -0.04533001447278906 1102 7 -0.01769480206000651 1102 8 -0.0641550082189255 1102 9 -0.04580274973863396 1102 10-11 -0.052473459813413115 1102 12-13 -0.06186214510712773 1102 14-15 -0.03125153316430129 1102 16-17 -0.04248926310406631 1102 18-19 0.05932784362709498 1102 20-21 -0.03194915076198157 1102 22-23 -0.06254208548575235 1102 24-25 -0.05429076727158133 1102 26-27 0.01608163928672468 1102 28-29 -0.04498508146814828 1102 30-31 -0.1494985050473403 1102 32-33 -9.887981186480488E-4 1102 34-35 0.007898780888687895 1102 36-37 -0.03882053961041976 1102 38-39 -0.015529450202556916 1102 40-41 -0.061730575417023203 1102 42-43 0.053193630960734595 1102 44-45 -0.13557110803500194 1102 46-47 -0.05035381107818182 1102 48-49 -0.06900636561134377 1102 50-51 -0.11392866232912269 1102 52-53 -0.1489733685220287 1102 54-55 -0.10115141717906795 1102 56-57 -0.14109879283570592 1102 58-59 0.019595315265206636 1102 60-61 -0.11580921927395593 1102 62-63 -0.06615316758659162 1102 64-65 -0.1978286695706828 1102 66-67 -0.013440569071626385 1102 68-69 -0.09782470325224324 1102 70-71 0.05388522200669854 1102 72-73 -0.342038916598689 1102 74-75 0.08099319360416501 1102 76-77 -0.21258072833649777 1102 78-79 -0.09936435795670917 1102 80-81 -0.18454955293458397 1102 82-83 -0.33373814202430196 1102 84-85 -0.08767685774034817 1102 86-87 0.3270893570894131 1102 88-89 0.1335591737830839 1102 90-91 0.4856649867114413 1103 1 0.06289661373050137 1103 2 0.06129132591450315 1103 3 -0.047518859534584124 1103 4 -0.04711526280383538 1103 5 -0.022744409028248924 1103 6 -0.07708402862559183 1103 7 -0.06835115545711545 1103 8 -0.10720412795901524 1103 9 -0.11239996946635245 1103 10-11 -0.0666821438737486 1103 12-13 0.021522076069402374 1103 14-15 -0.01408536739366184 1103 16-17 -0.08166292435466715 1103 18-19 -0.05279703314450046 1103 20-21 -0.08288089719284386 1103 22-23 -0.1423022939862193 1103 24-25 0.0012907311059962012 1103 26-27 -0.06641653473771925 1103 28-29 -0.01436456907745054 1103 30-31 -0.04167614552771681 1103 32-33 0.004212430334703754 1103 34-35 -0.06746543081995782 1103 36-37 -0.011125602703252468 1103 38-39 0.0957241504671913 1103 40-41 -0.07601443318483803 1103 42-43 -0.04460863176376506 1103 44-45 -0.1308542082087385 1103 46-47 0.06722332528983799 1103 48-49 0.013605779381038019 1103 50-51 -0.10712731130602293 1103 52-53 0.05260127835413897 1103 54-55 -0.13075071739328337 1103 56-57 -0.11357841614184849 1103 58-59 -0.1174024497680648 1103 60-61 0.03525643621066621 1103 62-63 0.11177877691953597 1103 64-65 -0.10498420284544352 1103 66-67 0.07257830762350181 1103 68-69 -0.04258122264094055 1103 70-71 0.10832482696265089 1103 72-73 0.2196655428815646 1103 74-75 0.03830354581367601 1103 76-77 0.03777653487699695 1103 78-79 -0.3332485195367525 1103 80-81 0.16078649401197254 1103 82-83 0.03520372709282782 1103 84-85 0.15650862768717388 1103 86-87 -0.09438979867545783 1103 88-89 -0.1669430842678139 1103 90-91 -0.014335013288558685 1104 1 -0.020788224610093664 1104 2 0.016019717978942083 1104 3 -0.049617691491839366 1104 4 -0.07247231096446427 1104 5 0.13581938460722398 1104 6 -0.001040517667917129 1104 7 -0.05657269473653059 1104 8 -0.02663135079641421 1104 9 -0.03176139819851187 1104 10-11 0.05667456610517263 1104 12-13 -0.1354264722823615 1104 14-15 -0.16184050541982486 1104 16-17 -0.0018408579328834662 1104 18-19 -0.04400430891983831 1104 20-21 0.04177261460041848 1104 22-23 0.047788061058511744 1104 24-25 0.025162148370235116 1104 26-27 -0.03477022805692087 1104 28-29 0.025467840177675782 1104 30-31 -0.12970865834277134 1104 32-33 -0.14319203337122133 1104 34-35 -0.0020915519880091438 1104 36-37 -0.19959014702901356 1104 38-39 -0.17218616326392322 1104 40-41 -0.12339234876974103 1104 42-43 -0.011762618878414344 1104 44-45 -0.1571265963630495 1104 46-47 -0.03346537164284058 1104 48-49 -0.15904155020368194 1104 50-51 -0.15083906084662146 1104 52-53 0.22891814997080218 1104 54-55 -9.358090356528237E-4 1104 56-57 -0.152240472660921 1104 58-59 -0.02675279352055071 1104 60-61 0.026722097895742536 1104 62-63 -0.32472795419914746 1104 64-65 -0.3447581179004402 1104 66-67 -0.34166255144258173 1104 68-69 -0.3289823699144421 1104 70-71 -0.1906007529474465 1104 72-73 -0.10506640484553742 1104 74-75 -0.31174633484421577 1104 76-77 -0.166517256369211 1104 78-79 -0.2013701011154012 1104 80-81 -0.3493819035398502 1104 82-83 -0.09685330567909034 1104 84-85 -0.11349258627944891 1104 86-87 -0.9129968160402342 1104 88-89 0.0042900922285156184 1104 90-91 0.16566498671144103 1201 1 -0.05447887618718994 1201 2 0.08073929564150006 1201 3 0.10094479335353412 1201 4 0.060455112460076066 1201 5 -0.0013802309213204467 1201 6 0.008252920778389239 1201 7 -0.014917530249704214 1201 8 -0.019193758799154637 1201 9 -0.08777128313150939 1201 10-11 0.006031396495089325 1201 12-13 -1.5696289028710453E-4 1201 14-15 0.0046865207476756154 1201 16-17 0.013894446962858353 1201 18-19 -0.048431477762797215 1201 20-21 0.027100191839373622 1201 22-23 0.025290604452806065 1201 24-25 0.04969044599273076 1201 26-27 -0.01629570930005997 1201 28-29 0.03144865082870041 1201 30-31 0.05587663229835016 1201 32-33 0.041750538320386 1201 34-35 0.05076297473992497 1201 36-37 0.1781485839345791 1201 38-39 0.1591661149751431 1201 40-41 0.10114385505367096 1201 42-43 0.16427873642835777 1201 44-45 0.11022710231747368 1201 46-47 0.08734072041092844 1201 48-49 0.04270445776849385 1201 50-51 -0.0046081595698197475 1201 52-53 0.03290329732101327 1201 54-55 0.06150999591254447 1201 56-57 0.0776803628837115 1201 58-59 0.11358488304780678 1201 60-61 -0.006431649955288776 1201 62-63 -0.07095985446861164 1201 64-65 -0.05462208005929625 1201 66-67 1.0124485349649603E-4 1201 68-69 0.2120967334913786 1201 70-71 0.16695999424459274 1201 72-73 0.040086425167409345 1201 74-75 0.01795164373853453 1201 76-77 -0.11699532445419436 1201 78-79 -0.17951284504854215 1201 80-81 -0.06837955874297563 1201 82-83 0.03065764848378194 1201 84-85 0.2182755232120357 1201 86-87 0.16296237296243277 1201 88-89 -0.32265053471210337 1201 90-91 -0.3714778704314199 1202 1 0.051278590893559794 1202 2 0.01853036744705605 1202 3 0.006823839744434679 1202 4 0.03786501532380271 1202 5 0.06624762447560784 1202 6 0.07216726586523947 1202 7 0.03536619719898226 1202 8 0.0672191174163217 1202 9 -0.006135100314885733 1202 10-11 -0.06767860378469948 1202 12-13 0.03186634065865235 1202 14-15 0.02678762996811912 1202 16-17 0.014112552106077203 1202 18-19 0.004807161057634346 1202 20-21 -0.12298030994234921 1202 22-23 -0.0905874499730146 1202 24-25 -0.04919829876448034 1202 26-27 0.012077035569070915 1202 28-29 -0.1898918246532233 1202 30-31 -0.09803120142795052 1202 32-33 -0.0372691331165953 1202 34-35 0.0049674053800359275 1202 36-37 -0.22309104109508837 1202 38-39 -0.14063450707158864 1202 40-41 -0.3203304027089473 1202 42-43 -0.07975373663776253 1202 44-45 -0.17391804255225196 1202 46-47 -0.1798351342184361 1202 48-49 -0.20919673247240667 1202 50-51 -0.0749241220385315 1202 52-53 -0.2257800425153036 1202 54-55 -0.1304200574094665 1202 56-57 -0.10483813978380851 1202 58-59 -0.2957772059216879 1202 60-61 -0.13442711138046093 1202 62-63 -0.27106192222598224 1202 64-65 -0.05846141000363758 1202 66-67 -0.09021068106859076 1202 68-69 -0.26640129559856973 1202 70-71 -0.33486949475913264 1202 72-73 -0.3109998573540409 1202 74-75 -0.04510596569453895 1202 76-77 -0.024498750091510146 1202 78-79 -0.2068372427363414 1202 80-81 -0.2667073799210584 1202 82-83 0.07834318851372046 1202 84-85 0.1059063332499619 1202 86-87 -0.3548714272243032 1202 88-89 0.06176504970348162 1202 90-91 -1.087761586715132 1203 1 0.038731153592458156 1203 2 0.1443237539152662 1203 3 -0.1627292606835482 1203 4 -0.1021678558228345 1203 5 -0.031047651828330913 1203 6 0.0038435156961611483 1203 7 -0.037405876618173295 1203 8 0.0620587557316199 1203 9 -0.10592250974696782 1203 10-11 -0.0015528563361684178 1203 12-13 -0.027244938891556103 1203 14-15 -0.018876594331523222 1203 16-17 -0.02956996059703698 1203 18-19 -0.045726484653677346 1203 20-21 0.0920105152306192 1203 22-23 0.028303761333845046 1203 24-25 0.05497107951656943 1203 26-27 0.024773771168163705 1203 28-29 -0.005160063928634884 1203 30-31 0.05738130849336898 1203 32-33 0.03176049279431936 1203 34-35 0.034148306814437035 1203 36-37 0.12126595322119016 1203 38-39 0.011636455728869066 1203 40-41 -0.019164405998104428 1203 42-43 -0.04820616982493675 1203 44-45 -0.03569399516318583 1203 46-47 -0.03404434466857964 1203 48-49 0.006356050728307139 1203 50-51 -0.04451662074555429 1203 52-53 0.03728676401207309 1203 54-55 -0.11663023984819176 1203 56-57 0.028912681186938016 1203 58-59 -0.005321005732838557 1203 60-61 -0.10177651022074485 1203 62-63 -0.10281276880815682 1203 64-65 -0.03549632748099896 1203 66-67 -0.021288760341171553 1203 68-69 -0.045503295838841495 1203 70-71 0.05011506147668143 1203 72-73 0.03873514739130002 1203 74-75 -0.17394853792350773 1203 76-77 0.06877895815490831 1203 78-79 0.2071475428336953 1203 80-81 0.19705971541196732 1203 82-83 8.924445056806007E-4 1203 84-85 0.10704785912544423 1203 86-87 -0.2928472828472195 1203 88-89 0.2416224472079307 1203 90-91 0.4856649867114413 1204 1 0.09201908697969685 1204 2 -0.014101663467499748 1204 3 -0.14769939571482382 1204 4 -0.2801156300505738 1204 5 -0.16447206373656797 1204 6 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-0.02005502440871254 2401 36-37 0.10225723363760153 2401 38-39 0.1274834481636944 2401 40-41 0.09273582216631127 2401 42-43 0.09437038161368605 2401 44-45 0.12027244161219386 2401 46-47 0.07906059856030367 2401 48-49 -0.027289889875518725 2401 50-51 0.2200620085019338 2401 52-53 0.05365760477892678 2401 54-55 0.05910373537657421 2401 56-57 0.14044278877351246 2401 58-59 0.11078844840282898 2401 60-61 0.03551416366758531 2401 62-63 -0.0026576213311670926 2401 64-65 -0.11158795048437753 2401 66-67 0.08470140530757675 2401 68-69 0.012630520200289652 2401 70-71 0.0057422023903441755 2401 72-73 -0.03153328246969522 2401 74-75 -0.1467572847182339 2401 76-77 -0.17207407946828823 2401 78-79 -0.16455808331105715 2401 80-81 0.28885026838030115 2401 82-83 0.07023897099506371 2401 84-85 0.24098628416877688 2401 86-87 -0.1539528377165098 2401 88-89 -0.20317001523158496 2401 90-91 0.08566498671144274 2402 1 -0.018902694373032602 2402 2 -0.06131960336680464 2402 3 0.17826240602181542 2402 4 0.14176974844655632 2402 5 0.1688193168164176 2402 6 0.0919579536232078 2402 7 0.05765323151443624 2402 8 0.06278944773720241 2402 9 0.1206859286104418 2402 10-11 0.14775755308356509 2402 12-13 0.1723789817767596 2402 14-15 0.06517775553061966 2402 16-17 0.0622624405363581 2402 18-19 0.018055806282482934 2402 20-21 0.004989535184812155 2402 22-23 -0.0029441584386518116 2402 24-25 0.029976871902000823 2402 26-27 0.06054258971246185 2402 28-29 0.06859803057523095 2402 30-31 0.0906592722983035 2402 32-33 0.01276171184376551 2402 34-35 0.051178929272403195 2402 36-37 0.08678767129912046 2402 38-39 -0.4342562147899045 2402 40-41 0.12226797740574114 2402 42-43 0.10149034607731267 2402 44-45 0.10765530895686481 2402 46-47 0.19897333521124239 2402 48-49 -0.05740336183788486 2402 50-51 0.14195656932299272 2402 52-53 -0.03427919278558278 2402 54-55 0.21436954517570683 2402 56-57 0.14273972509077737 2402 58-59 0.07487806147446463 2402 60-61 -0.0076605290160216555 2402 62-63 0.039657136143858907 2402 64-65 0.21233907274795172 2402 66-67 0.1676091440772396 2402 68-69 0.30216660198605894 2402 70-71 0.19711776943812254 2402 72-73 0.07480155769696495 2402 74-75 0.09237464112064941 2402 76-77 0.26010070963842225 2402 78-79 0.06213158773370253 2402 80-81 -0.08117451437028933 2402 82-83 0.12552654056835166 2402 84-85 -0.032005060401886 2402 86-87 -0.35340018452161814 2402 88-89 -0.6077536668740606 2402 90-91 -0.5424867880743562 2403 1 0.054535122346038634 2403 2 0.056356151946424404 2403 3 0.16123218924308702 2403 4 0.1356691946967885 2403 5 0.14295528063464502 2403 6 -0.005690001590672011 2403 7 0.01914869158228072 2403 8 0.11900837075744164 2403 9 0.1131974946331269 2403 10-11 0.08631729376328678 2403 12-13 0.08577670040082808 2403 14-15 0.11497826368346153 2403 16-17 0.048678127237479885 2403 18-19 0.09728099006188273 2403 20-21 0.1532679220311337 2403 22-23 0.12452962333100004 2403 24-25 0.06288144684986463 2403 26-27 0.12232833177156266 2403 28-29 0.13884236293727525 2403 30-31 0.04738044138129993 2403 32-33 0.1626812100435373 2403 34-35 0.028921736284111432 2403 36-37 0.06486266257597606 2403 38-39 0.09447989304175763 2403 40-41 0.08751206677918333 2403 42-43 0.14741953050429402 2403 44-45 0.1101314076517923 2403 46-47 -0.04446797683505821 2403 48-49 0.13895606729295196 2403 50-51 0.14341844024071548 2403 52-53 0.03128738404880238 2403 54-55 -0.014807626568604348 2403 56-57 0.1801357812244646 2403 58-59 -0.0384369818780641 2403 60-61 0.06946000015442877 2403 62-63 0.09774136076949702 2403 64-65 0.08397652353990281 2403 66-67 -0.0411425179231415 2403 68-69 -0.05208918303311805 2403 70-71 -0.08733768010122134 2403 72-73 -0.05140960338992073 2403 74-75 0.10351084467248484 2403 76-77 0.1723218049564963 2403 78-79 0.06142427654118876 2403 80-81 -0.11462564231300121 2403 82-83 0.25623693417853133 2403 84-85 -0.13160644195654925 2403 86-87 0.213505397189671 2403 88-89 0.5309071751836285 2403 90-91 0.4856649867114413 2404 1 0.10232497098405702 2404 2 -0.041495085041631796 2404 3 -0.13288204260850023 2404 4 -0.1185263873911282 2404 5 0.03372500004121548 2404 6 0.10963898532225613 2404 7 0.04215352751572965 2404 8 0.03441368755206753 2404 9 0.07248480918737243 2404 10-11 -0.027025323241716137 2404 12-13 0.01843308274010269 2404 14-15 0.041422810963588574 2404 16-17 -0.07405204065410231 2404 18-19 0.043480803591570805 2404 20-21 -0.036866056548682025 2404 22-23 -0.0354403911494785 2404 24-25 0.013160298520588753 2404 26-27 0.015258596655037593 2404 28-29 0.03621111652324771 2404 30-31 0.032200699024535595 2404 32-33 0.09631458772220469 2404 34-35 0.13770110241794242 2404 36-37 0.014680269012060876 2404 38-39 0.12985737010176734 2404 40-41 0.1388448762824055 2404 42-43 0.12804948515892534 2404 44-45 0.04387833497454352 2404 46-47 0.0649305886253515 2404 48-49 0.11939064085171935 2404 50-51 0.17846731874038113 2404 52-53 0.15656939763005795 2404 54-55 -0.008187699993357 2404 56-57 0.012395399872957569 2404 58-59 0.030102761273319345 2404 60-61 0.31766119028770845 2404 62-63 0.12416313169706683 2404 64-65 0.12797542078271817 2404 66-67 0.01816728336136464 2404 68-69 0.04936495710170874 2404 70-71 0.15491268477139641 2404 72-73 0.18454933683314323 2404 74-75 0.06471714313213539 2404 76-77 0.18642248522688476 2404 78-79 0.192461728112427 2404 80-81 0.054070526383171114 2404 82-83 -0.16019479307626483 2404 84-85 0.05699325317064563 2404 86-87 -0.07018068849921377 2404 88-89 -0.009978022039589973 2404 90-91 0.2805367815832369 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 25.0 31 405.0 32 2687.0 33 4892.0 34 6460.0 35 7980.0 36 11294.0 37 18287.0 38 59756.0 39 321792.0 40 179821.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.032810617558884 17.22614480949594 24.574379808248793 32.16666476469639 2 24.6883163047342 17.296121523214442 29.63302363349976 28.382538538551593 3 28.103567172427734 18.65376370029948 21.167461961952984 32.0752071653198 4 28.64302028532815 22.284190225285663 19.967101348388244 29.105688140997948 5 27.632421963518038 21.196643620221096 21.190285605291173 29.980648810969697 6 28.229260236811605 22.716209188472757 22.645781946172068 26.408748628543577 7 26.90695615741141 23.746370633144167 22.842717382975845 26.503955826468577 8 25.5037911701845 24.083671476477793 23.57193278763089 26.840604565706823 9 25.6200287251854 23.441674994579383 23.449011165652372 27.48928511458284 10-11 25.42790255608503 23.299923866846864 23.718574696078733 27.553598880989373 12-13 26.337995334195195 22.977947469754596 22.879642777376553 27.80441441867365 14-15 26.34549453129203 22.259002704601734 23.019600618846788 28.375902145259445 16-17 26.2693613781568 22.454552420202837 23.034354474004683 28.24173172763568 18-19 26.539658525690456 22.43401114119847 22.87996882942424 28.146361503686833 20-21 26.41706295575963 22.37491420755495 22.98960383045946 28.218419006225965 22-23 26.153449875203577 22.61439943658206 23.210015014696797 28.02213567351756 24-25 26.66486251200279 22.52897380008771 22.789489386190716 28.016674301718787 26-27 26.40271666566134 22.266012823627037 23.059705020712457 28.27156548999917 28-29 26.454151376184182 22.556199146069687 23.23275714502306 27.75689233272307 30-31 26.962300709088048 22.49363581453015 22.89142287944036 27.652640596941442 32-33 26.765377930145434 22.311275188024744 22.984999444914838 27.938347436914984 34-35 26.611636857672643 22.592460026304803 23.278391377057346 27.5175117389652 36-37 26.965165766196424 22.39601254091952 22.91703328982009 27.72178840306396 38-39 27.101853605319885 22.3224568312111 22.948117716668495 27.62757184680052 40-41 26.808372377745858 22.52536905798978 23.220304664139572 27.445953900124785 42-43 27.273372025674064 22.413484004933075 22.826904967355798 27.48623900203706 44-45 27.115753438141432 22.283135207832323 22.950610747371595 27.650500606654653 46-47 26.88052504834332 22.399927416701374 23.30798383491235 27.41156370004296 48-49 27.39441084327871 22.438997302137466 22.81949772555843 27.34709412902539 50-51 27.35137104586483 22.251476780784063 22.887855747536033 27.509296425815077 52-53 27.281862202107792 22.455586500828137 23.14341551413109 27.11913578293298 54-55 27.862961460216034 22.158744300464477 22.730187464001066 27.24810677531842 56-57 27.641423462472144 22.170420345680526 22.82563580251641 27.362520389330925 58-59 27.431989721376844 22.474847790278858 22.942405470408236 27.150757017936066 60-61 28.077997557820588 22.242674367624094 22.476384414982082 27.202943659573236 62-63 27.907170782874037 22.058461269508282 22.540208512126124 27.49415943549156 64-65 27.614097418689827 22.30651139329342 22.723644099392505 27.35574708862425 66-67 28.16984829576476 22.09363544185321 22.36101903127681 27.375497231105218 68-69 28.193221549118835 21.937992734843593 22.417885474319878 27.450900241717697 70-71 27.9844088403229 22.067582201220713 22.631792675723567 27.316216282732817 72-73 28.77068938937396 21.942665339929647 21.92339495185907 27.36325031883733 74-75 28.55597139961807 21.84634763756162 21.89802534630121 27.699655616519102 76-77 28.40580804743113 21.942089462056984 22.373066973810918 27.279035516700965 78-79 29.162712606759616 21.776435113175022 21.755097227759464 27.305755052305898 80-81 29.041036098399342 21.673361659176006 21.541735151097683 27.743867091326972 82-83 29.229091720828006 21.650511277291734 21.650511277291734 27.469885724588533 84-85 31.088042020311317 20.900821695465456 20.27637822788805 27.734758056335167 86-87 31.074530882564666 20.028474988402042 20.12925725072387 28.767736878309417 88-89 31.82609599542631 20.020269743509783 19.661651204490525 28.49198305657337 90-91 30.689997517994538 17.00173740382229 18.497145693720526 33.81111938446264 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 3.0 9 4.5 10 5.0 11 5.0 12 8.0 13 21.0 14 37.0 15 43.5 16 51.5 17 80.5 18 115.0 19 156.5 20 217.5 21 288.0 22 408.0 23 584.5 24 793.0 25 1023.0 26 1299.5 27 1660.5 28 2105.0 29 2597.5 30 3163.5 31 3850.0 32 4657.0 33 5398.5 34 6292.5 35 7509.0 36 8860.5 37 10139.0 38 11515.5 39 13206.5 40 14929.0 41 16885.0 42 18920.5 43 20800.5 44 22736.5 45 24584.5 46 26575.0 47 28836.0 48 30968.0 49 32951.0 50 34673.5 51 36335.5 52 38067.0 53 39727.0 54 40979.5 55 41811.0 56 42641.5 57 43197.0 58 43502.5 59 43385.5 60 42609.0 61 41452.0 62 39984.0 63 38066.5 64 35582.0 65 32656.5 66 29079.0 67 25710.0 68 22578.0 69 19071.5 70 15933.5 71 13214.0 72 10778.5 73 8540.0 74 6477.5 75 4904.5 76 3737.0 77 2706.0 78 1915.0 79 1368.5 80 965.5 81 653.5 82 436.5 83 290.5 84 199.0 85 145.0 86 103.0 87 72.5 88 48.0 89 28.5 90 18.0 91 11.0 92 6.0 93 3.0 94 2.5 95 4.0 96 4.0 97 3.0 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.019074044789769793 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.06383915069480539 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.4374014375793678E-4 68-69 5.450200158600825E-4 70-71 6.152748124950009E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0020459660369637864 82-83 0.0 84-85 0.0 86-87 0.0015996928589710774 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 6334.0 32-33 14939.0 34-35 16553.0 36-37 18394.0 38-39 20813.0 40-41 22587.0 42-43 24841.0 44-45 25385.0 46-47 24877.0 48-49 25878.0 50-51 26981.0 52-53 26650.0 54-55 26326.0 56-57 25725.0 58-59 24896.0 60-61 24463.0 62-63 24017.0 64-65 22959.0 66-67 22059.0 68-69 21170.0 70-71 19853.0 72-73 19350.0 74-75 18129.0 76-77 17023.0 78-79 16053.0 80-81 15397.0 82-83 13972.0 84-85 13394.0 86-87 12240.0 88-89 11324.0 90-91 10817.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.83929038098614 #Duplication Level Percentage of deduplicated Percentage of total 1 99.85205624023016 99.69158438106899 2 0.1384642028776235 0.2764833551694166 3 0.006709339990158757 0.020095672306266684 4 0.0022442786027546085 0.008962687324650047 5 2.7709959022035913E-4 0.001383271322623135 6 2.4883870908504776E-4 0.0014906328080623089 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0014672342145976762 0.0 1.6302602384418624E-4 0.0 0.0 2 0.0014672342145976762 0.0 1.6302602384418624E-4 0.0 0.0 3 0.0016302602384418625 0.0 1.6302602384418624E-4 0.0 0.0 4 0.0016302602384418625 0.0 1.6302602384418624E-4 0.0 0.0 5 0.0016302602384418625 0.0 1.6302602384418624E-4 0.0 1.6302602384418624E-4 6 0.0017932862622860486 0.0 1.6302602384418624E-4 0.0 1.6302602384418624E-4 7 0.001956312286130235 0.0 1.6302602384418624E-4 0.0 1.6302602384418624E-4 8 0.007499197096832567 0.0 1.6302602384418624E-4 0.0 1.6302602384418624E-4 9 0.0076622231206767534 0.0 1.6302602384418624E-4 0.0 1.6302602384418624E-4 10-11 0.00782524914452094 0.0 1.6302602384418624E-4 0.0 1.6302602384418624E-4 12-13 0.00782524914452094 0.0 1.6302602384418624E-4 0.0 1.6302602384418624E-4 14-15 0.00782524914452094 0.0 1.6302602384418624E-4 0.0 1.6302602384418624E-4 16-17 0.00782524914452094 0.0 1.6302602384418624E-4 0.0 2.4453903576627937E-4 18-19 0.007906762156443033 0.0 2.4453903576627937E-4 0.0 3.2605204768837247E-4 20-21 0.008314327216053498 0.0 3.2605204768837247E-4 0.0 3.2605204768837247E-4 22-23 0.008803405287586058 0.0 3.2605204768837247E-4 0.0 4.075650596104656E-4 24-25 0.009047944323352337 0.0 3.2605204768837247E-4 0.0 4.890780715325587E-4 26-27 0.009292483359118617 0.0 3.2605204768837247E-4 0.0 4.890780715325587E-4 28-29 0.009455509382962803 0.0 3.2605204768837247E-4 0.0 5.705910834546519E-4 30-31 0.009537022394884895 0.0 3.2605204768837247E-4 0.0 6.521040953767449E-4 32-33 0.009781561430651176 0.0 3.2605204768837247E-4 0.0 6.521040953767449E-4 34-35 0.01018912649026164 0.0 3.2605204768837247E-4 0.0 6.521040953767449E-4 36-37 0.010352152514105827 0.0 3.2605204768837247E-4 0.0 6.521040953767449E-4 38-39 0.010678204561794198 0.0 3.2605204768837247E-4 0.0 6.521040953767449E-4 40-41 0.010922743597560479 0.0 3.2605204768837247E-4 0.0 6.521040953767449E-4 42-43 0.011574847692937224 0.0 3.2605204768837247E-4 0.0 6.521040953767449E-4 44-45 0.01173787371678141 0.0 3.2605204768837247E-4 0.0 6.521040953767449E-4 46-47 0.011819386728703503 0.0 3.2605204768837247E-4 0.0 6.521040953767449E-4 48-49 0.011982412752547689 0.0 3.2605204768837247E-4 0.0 6.521040953767449E-4 50-51 0.01255300383600234 0.0 3.2605204768837247E-4 0.0 6.521040953767449E-4 52-53 0.013042081907534898 0.0 3.2605204768837247E-4 0.0 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