##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-28C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 191877 Sequences flagged as poor quality 0 Sequence length 31-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.471703226546175 40.0 40.0 40.0 40.0 40.0 2 39.282550800773414 40.0 40.0 40.0 40.0 40.0 3 39.23753237751268 40.0 40.0 40.0 40.0 40.0 4 39.099600264752944 40.0 40.0 40.0 40.0 40.0 5 39.119706895563304 40.0 40.0 40.0 40.0 40.0 6 39.28798136306071 40.0 40.0 40.0 40.0 40.0 7 39.29240086096822 40.0 40.0 40.0 40.0 40.0 8 39.27013659792471 40.0 40.0 40.0 40.0 40.0 9 39.303491299113496 40.0 40.0 40.0 40.0 40.0 10-11 39.316749792836035 40.0 40.0 40.0 40.0 40.0 12-13 39.238293281633545 40.0 40.0 40.0 40.0 40.0 14-15 39.26585260349078 40.0 40.0 40.0 40.0 40.0 16-17 39.373682098427636 40.0 40.0 40.0 40.0 40.0 18-19 39.419961746327076 40.0 40.0 40.0 40.0 40.0 20-21 39.373723791804125 40.0 40.0 40.0 40.0 40.0 22-23 39.44229897277944 40.0 40.0 40.0 40.0 40.0 24-25 39.399479875128335 40.0 40.0 40.0 40.0 40.0 26-27 39.37185801320638 40.0 40.0 40.0 40.0 40.0 28-29 39.395300114135615 40.0 40.0 40.0 40.0 40.0 30-31 39.373400668136355 40.0 40.0 40.0 40.0 40.0 32-33 39.36966819587189 40.0 40.0 40.0 40.0 40.0 34-35 39.36366097199316 40.0 40.0 40.0 40.0 40.0 36-37 39.34147885854525 40.0 40.0 40.0 40.0 40.0 38-39 39.26861511932177 40.0 40.0 40.0 40.0 40.0 40-41 39.324374951538765 40.0 40.0 40.0 40.0 40.0 42-43 39.32893038824437 40.0 40.0 40.0 40.0 40.0 44-45 39.22421927762213 40.0 40.0 40.0 40.0 40.0 46-47 39.180312199754766 40.0 40.0 40.0 40.0 40.0 48-49 39.2785828644962 40.0 40.0 40.0 40.0 40.0 50-51 39.23148102911556 40.0 40.0 40.0 40.0 40.0 52-53 39.19015348061239 40.0 40.0 40.0 40.0 40.0 54-55 39.08272171015006 40.0 40.0 40.0 40.0 40.0 56-57 39.112923157154825 40.0 40.0 40.0 40.0 40.0 58-59 39.03790301812971 40.0 40.0 40.0 40.0 40.0 60-61 38.95858223819228 40.0 40.0 40.0 40.0 40.0 62-63 39.23135924709345 40.0 40.0 40.0 40.0 40.0 64-65 39.23341430415026 40.0 40.0 40.0 40.0 40.0 66-67 39.25995022574526 40.0 40.0 40.0 40.0 40.0 68-69 39.24401596564753 40.0 40.0 40.0 40.0 40.0 70-71 39.2518946452892 40.0 40.0 40.0 40.0 40.0 72-73 39.24052125490783 40.0 40.0 40.0 40.0 40.0 74-75 39.24392200600777 40.0 40.0 40.0 40.0 40.0 76-77 39.24173030977917 40.0 40.0 40.0 40.0 40.0 78-79 39.22632723824496 40.0 40.0 40.0 40.0 40.0 80-81 39.20667419552055 40.0 40.0 40.0 40.0 40.0 82-83 39.19917499815378 40.0 40.0 40.0 40.0 40.0 84-85 39.181861273552066 40.0 40.0 40.0 40.0 40.0 86-87 39.11154663522531 40.0 40.0 40.0 40.0 40.0 88-89 39.20216978373165 40.0 40.0 40.0 40.0 40.0 90-91 39.29880441784104 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1812908071439452 1101 2 0.03032238872148696 1101 3 0.23971503855720755 1101 4 0.15413710774871703 1101 5 0.049911844603499844 1101 6 0.03421937539300757 1101 7 0.3278006760417327 1101 8 0.2251669282468356 1101 9 0.015766445302254795 1101 10-11 -0.01250050315171336 1101 12-13 0.05943561900331673 1101 14-15 0.13564439491061364 1101 16-17 0.1333544399861708 1101 18-19 0.04876969514348417 1101 20-21 0.20069153076385504 1101 22-23 0.15206337768448464 1101 24-25 0.040891721626323374 1101 26-27 0.06415055289718907 1101 28-29 0.06366591986312642 1101 30-31 0.261404582689039 1101 32-33 0.06338361744604981 1101 34-35 0.09198200209380047 1101 36-37 -0.0831932891617484 1101 38-39 -0.06724613822616021 1101 40-41 -0.06177892361893811 1101 42-43 -0.02695525363645146 1101 44-45 0.09966315036607654 1101 46-47 0.02258503406945067 1101 48-49 0.02884562531038881 1101 50-51 0.1252920701962097 1101 52-53 0.2868537553447439 1101 54-55 0.16124425497355332 1101 56-57 0.12939481471607905 1101 58-59 0.17424283156028508 1101 60-61 0.15377484469907188 1101 62-63 0.17257447040699248 1101 64-65 0.2381957065797593 1101 66-67 0.15733703147031264 1101 68-69 0.17224807390191188 1101 70-71 0.5886265687611001 1101 72-73 0.07425056089176252 1101 74-75 0.3198188883274895 1101 76-77 -0.05420685921137647 1101 78-79 -0.33679083303527335 1101 80-81 0.247011154247744 1101 82-83 0.5436594132454573 1101 84-85 0.02042953838552819 1101 86-87 0.5620906056478532 1101 88-89 0.24660228522487415 1101 90-91 -0.3538347069597094 1102 1 0.1963658385351863 1102 2 0.06917153346856963 1102 3 0.1687473199674443 1102 4 0.21533464276758707 1102 5 0.18209157255816422 1102 6 0.024022198614204626 1102 7 0.01393895670653933 1102 8 0.05748162947823943 1102 9 -0.008579404721807293 1102 10-11 0.10527363191565087 1102 12-13 0.09352270390620987 1102 14-15 0.0235550592469167 1102 16-17 0.08784709606485563 1102 18-19 -0.09752975736437719 1102 20-21 0.04626111437310243 1102 22-23 0.03761110503123177 1102 24-25 0.07609694976009962 1102 26-27 0.059583896724419105 1102 28-29 0.10104591848805455 1102 30-31 0.1404714216004237 1102 32-33 0.04243953596146355 1102 34-35 0.141601048930184 1102 36-37 -0.021387385039126627 1102 38-39 0.08440147653084296 1102 40-41 0.21815066801703864 1102 42-43 0.09374730545956567 1102 44-45 0.1505956549960885 1102 46-47 0.16551694177320542 1102 48-49 0.1426206004276409 1102 50-51 0.01509960581337566 1102 52-53 0.1943742879832584 1102 54-55 0.08260446870905724 1102 56-57 -0.0014219404987372286 1102 58-59 0.169830052681057 1102 60-61 -0.16052553285878446 1102 62-63 0.35955749770772627 1102 64-65 0.3586347727243151 1102 66-67 -0.24829389320471051 1102 68-69 -0.015636023483452277 1102 70-71 0.12838725841515952 1102 72-73 0.38351581803888024 1102 74-75 0.24786263438458178 1102 76-77 -0.1099785828871589 1102 78-79 0.3882048062460868 1102 80-81 0.6979620268853637 1102 82-83 0.18056864999113742 1102 84-85 0.19210709829838635 1102 86-87 0.554960480870669 1102 88-89 -0.07339771477512613 1102 90-91 0.44616529304028774 1103 1 -0.05564142293879826 1103 2 -0.06636321700094072 1103 3 0.0983256427618926 1103 4 0.07221468050218505 1103 5 0.19107268611181638 1103 6 0.0955647744880821 1103 7 0.012852868555867758 1103 8 -0.02797122924850015 1103 9 0.13747160312970408 1103 10-11 -0.00809726196622762 1103 12-13 0.13920106516628294 1103 14-15 -0.05085590328130252 1103 16-17 0.17641549612125118 1103 18-19 0.11562896641242304 1103 20-21 0.048363669639151396 1103 22-23 0.10217069106455057 1103 24-25 0.12482471236629777 1103 26-27 0.07741384386464745 1103 28-29 0.04726714977087454 1103 30-31 0.14422051435130356 1103 32-33 0.11437459521911819 1103 34-35 0.09850732381016769 1103 36-37 0.2321704565951066 1103 38-39 0.14986876641594904 1103 40-41 0.17764591328597845 1103 42-43 0.07980261954789825 1103 44-45 0.01700576735952808 1103 46-47 0.1845864515714979 1103 48-49 0.1418545030073446 1103 50-51 0.1516611056459638 1103 52-53 0.400925568520222 1103 54-55 0.16582559063461844 1103 56-57 0.2118374100904603 1103 58-59 0.023366351868780555 1103 60-61 -0.002075662178405935 1103 62-63 0.16225827880528954 1103 64-65 0.016726907509664102 1103 66-67 -0.01148726725505611 1103 68-69 -0.004748964401571243 1103 70-71 -0.2683598020231628 1103 72-73 -0.3815649428256549 1103 74-75 0.015986988602669783 1103 76-77 -0.433155900498555 1103 78-79 -0.4794426034574002 1103 80-81 -0.21141774996429064 1103 82-83 0.321437191023243 1103 84-85 -0.46145813193683693 1103 86-87 0.17112209703228132 1103 88-89 0.6466022852248727 1103 90-91 -0.12526327838828166 1104 1 0.22082992344713404 1104 2 0.3600539806911698 1104 3 0.1927636429259323 1104 4 0.1698664516749986 1104 5 0.0653071139685153 1104 6 0.06969391786746115 1104 7 5.468037347782229E-4 1104 8 0.23168138219152468 1104 9 0.15784557225406814 1104 10-11 0.024167659890593995 1104 12-13 -0.005381805769005155 1104 14-15 0.20193731566186557 1104 16-17 0.012154973113496226 1104 18-19 0.13225353602656043 1104 20-21 0.113710346817534 1104 22-23 0.12161735334576207 1104 24-25 0.049963933930705196 1104 26-27 0.11426173708711218 1104 28-29 0.09964157417947206 1104 30-31 0.1258886288887595 1104 32-33 0.028541664307454084 1104 34-35 0.19690825634017273 1104 36-37 0.19866177252093564 1104 38-39 0.10439677357097565 1104 40-41 0.1633560385807371 1104 42-43 0.08156561867679102 1104 44-45 0.06970607387034988 1104 46-47 0.29634445087090455 1104 48-49 0.14499568363693527 1104 50-51 0.2548755332077519 1104 52-53 0.17640294311345173 1104 54-55 0.3077217195152855 1104 56-57 0.2068086474676747 1104 58-59 0.17578497067012222 1104 60-61 0.17021534350824652 1104 62-63 0.11156430384848193 1104 64-65 0.15123746255339654 1104 66-67 0.25646089961956164 1104 68-69 -0.05098281809477356 1104 70-71 0.03409471989780144 1104 72-73 -0.15863036061940505 1104 74-75 0.3343546057304465 1104 76-77 -0.09633069046206799 1104 78-79 0.07046262333536646 1104 80-81 0.0531860672711062 1104 82-83 -0.1781414186605801 1104 84-85 0.17556963059812603 1104 86-87 0.3231039909496616 1104 88-89 -2.9748810907342076 1104 90-91 -0.16921932234432546 1201 1 0.09444866962959964 1201 2 0.15215792871715905 1201 3 -0.17594089369465138 1201 4 -0.004268702969902449 1201 5 -0.08017570017996434 1201 6 0.1609816073560495 1201 7 0.15535828688209108 1201 8 -0.16811871775850307 1201 9 0.12341834142503672 1201 10-11 0.12134129529511029 1201 12-13 -0.08478654784241257 1201 14-15 0.056343445930068015 1201 16-17 0.14486639518023736 1201 18-19 0.020753730373833434 1201 20-21 -0.10995559798784171 1201 22-23 0.0777503979345795 1201 24-25 -0.026664418559718683 1201 26-27 0.038299718096610036 1201 28-29 0.03400772443767153 1201 30-31 0.19774159129644886 1201 32-33 0.14891729085536554 1201 34-35 0.1557780853212165 1201 36-37 0.1423954829967542 1201 38-39 0.07848251199731493 1201 40-41 0.1202196819256045 1201 42-43 0.07114564524235334 1201 44-45 0.15772910651798355 1201 46-47 0.2351646304474002 1201 48-49 0.10718871520924012 1201 50-51 0.19777739797955007 1201 52-53 0.21658426124259478 1201 54-55 0.09561091421882395 1201 56-57 0.19966055220904622 1201 58-59 -0.04477052687479244 1201 60-61 0.321254670919636 1201 62-63 -0.05266816549369935 1201 64-65 -0.14654316422722502 1201 66-67 0.01676013177854685 1201 68-69 0.24094250804107276 1201 70-71 -2.174406324044753E-4 1201 72-73 0.07499667959433509 1201 74-75 0.6149009888590555 1201 76-77 0.4856996229287205 1201 78-79 0.05357864809051449 1201 80-81 0.5769871113898972 1201 82-83 -0.3006115396822082 1201 84-85 0.2751943813856741 1201 86-87 0.3267964880007952 1201 88-89 0.6466022852248727 1201 90-91 0.44616529304028774 1202 1 0.23307665293524593 1202 2 0.18192412669925062 1202 3 -0.128750975140413 1202 4 0.07365990029602898 1202 5 -0.14346027410921636 1202 6 0.25569371076380065 1202 7 0.222962322702017 1202 8 0.002293373493166939 1202 9 0.07565303488911468 1202 10-11 0.1469663206202938 1202 12-13 0.2733762626442342 1202 14-15 0.0757283480735893 1202 16-17 0.048318723070345015 1202 18-19 -0.02270832238357201 1202 20-21 -0.15277337828688076 1202 22-23 -0.07973572386939054 1202 24-25 -0.051920709333252546 1202 26-27 0.10649535001037691 1202 28-29 0.1842123518536951 1202 30-31 -0.03022955826390472 1202 32-33 0.16299058384311138 1202 34-35 0.003019950404471672 1202 36-37 -0.29470986617815953 1202 38-39 -0.3767608646992997 1202 40-41 -0.17190534940558422 1202 42-43 -0.23389316777804225 1202 44-45 -0.1577682959494311 1202 46-47 -0.10998361851642358 1202 48-49 0.09765125839653876 1202 50-51 -0.0162031734292043 1202 52-53 -0.1834068452871307 1202 54-55 0.23923320631027423 1202 56-57 -0.06131091964103774 1202 58-59 0.018885992873912016 1202 60-61 0.013454408955958286 1202 62-63 0.32441258006880247 1202 64-65 0.11271885319924024 1202 66-67 0.2649755445943853 1202 68-69 0.13697802841670637 1202 70-71 0.27711157147312093 1202 72-73 0.05373076930010967 1202 74-75 -0.07473091902671314 1202 76-77 0.11409340140416901 1202 78-79 -0.0515527661773163 1202 80-81 -0.35816071216420653 1202 82-83 -0.35500515863198956 1202 84-85 -0.1515966054053166 1202 86-87 -0.21055178464159496 1202 88-89 -0.04904988868817384 1202 90-91 0.44616529304028774 1203 1 -0.08450787720158814 1203 2 0.1142020112783868 1203 3 0.12611728223830454 1203 4 -0.05869337347983361 1203 5 -0.0810455581380154 1203 6 -0.03136573361781814 1203 7 -0.16153765845068335 1203 8 0.1516544500508985 1203 9 0.12702323353965284 1203 10-11 0.09719437294039324 1203 12-13 -0.13056282288481214 1203 14-15 -0.03503345975934735 1203 16-17 0.02079035788508321 1203 18-19 -0.07508119735059182 1203 20-21 -0.004192579915290651 1203 22-23 -0.008319537808844757 1203 24-25 -0.2121114440009748 1203 26-27 0.07607580913058598 1203 28-29 0.034267009021320405 1203 30-31 -0.012245465384410181 1203 32-33 -0.0178428772011614 1203 34-35 0.0864465673291761 1203 36-37 0.01533372807096356 1203 38-39 0.03595801642125451 1203 40-41 -0.06587730268005743 1203 42-43 0.03024880831598864 1203 44-45 -0.19421562683901783 1203 46-47 0.21624222420271622 1203 48-49 -0.09628173484228597 1203 50-51 -0.04649459708517156 1203 52-53 0.20241610243126473 1203 54-55 0.2930334228539806 1203 56-57 0.0939464853921379 1203 58-59 -0.06736863882547794 1203 60-61 0.11339210314972803 1203 62-63 -0.12734252119344802 1203 64-65 0.08946351619324844 1203 66-67 0.14155251843502725 1203 68-69 0.19136476831221927 1203 70-71 0.13975671306064186 1203 72-73 -0.0012947107939638158 1203 74-75 0.397165372042771 1203 76-77 0.4476957689048362 1203 78-79 0.5103204476140704 1203 80-81 0.7568180644648095 1203 82-83 0.4446071631835551 1203 84-85 0.7083657298112556 1203 86-87 0.2993004321071737 1203 88-89 0.31326895189154413 1203 90-91 0.44616529304028774 1204 1 -1.5282318967280162E-4 1204 2 0.02813816915993783 1204 3 0.08779855025629502 1204 4 -0.1395710249992348 1204 5 -0.15677814962548098 1204 6 -0.014330200546972094 1204 7 0.03938142276723511 1204 8 -0.22235701415989695 1204 9 0.005889974241341633 1204 10-11 -0.036614579314502294 1204 12-13 -0.09789393516751232 1204 14-15 -0.044886100154798214 1204 16-17 -0.10237325577175937 1204 18-19 -0.04746015196113973 1204 20-21 -0.09304582231268199 1204 22-23 -0.07456218571997653 1204 24-25 -0.0065109564167897815 1204 26-27 0.12671127639252688 1204 28-29 0.04544704499187446 1204 30-31 -0.12414304279401733 1204 32-33 -6.90599620909893E-4 1204 34-35 -0.04445060716721372 1204 36-37 -0.021922841844435936 1204 38-39 0.13683589192445567 1204 40-41 0.07100254355119517 1204 42-43 0.11027605802753015 1204 44-45 0.27148890552867755 1204 46-47 0.007948630991627681 1204 48-49 -0.19725889538639052 1204 50-51 -0.11375981078590769 1204 52-53 -0.07732349381657855 1204 54-55 -0.2174970003243999 1204 56-57 0.15000975340271339 1204 58-59 -0.22675200903403692 1204 60-61 -0.11812877598318039 1204 62-63 -0.12273149561175956 1204 64-65 -0.10275848886359995 1204 66-67 -0.3703066870657068 1204 68-69 -0.1354329320192278 1204 70-71 -0.6340614776452682 1204 72-73 -0.36830048071326615 1204 74-75 -0.13504636716040608 1204 76-77 -0.09336521689299104 1204 78-79 -0.07278939668360351 1204 80-81 0.3351659993834559 1204 82-83 0.4332590341883673 1204 84-85 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-0.6587016573983817 2401 66-67 0.07637375365005994 2401 68-69 -0.2826011997131559 2401 70-71 -0.032410136685008695 2401 72-73 -0.17441585825721972 2401 74-75 -0.2384681842083367 2401 76-77 -0.5019508934130528 2401 78-79 -0.6665639544050279 2401 80-81 -0.9690295771522415 2401 82-83 0.40064720157653255 2401 84-85 -0.1407609140263304 2401 86-87 0.4495586363383879 2401 88-89 0.6466022852248727 2401 90-91 0.44616529304028774 2402 1 0.0380104457885011 2402 2 -0.02380777970379455 2402 3 0.030472678042968937 2402 4 0.03622111923309035 2402 5 0.05526999978884106 2402 6 0.09211671094519147 2402 7 0.039795263024892336 2402 8 0.140113072386697 2402 9 0.13779705810696896 2402 10-11 -0.022777920264893226 2402 12-13 0.09421790966037946 2402 14-15 0.051367745860645186 2402 16-17 0.21608581902536628 2402 18-19 -0.003988931852106248 2402 20-21 0.02614916842089343 2402 22-23 0.029950472393991845 2402 24-25 -0.1341626828503948 2402 26-27 -0.1652260358049631 2402 28-29 0.0574574089913682 2402 30-31 0.01578894345929882 2402 32-33 -0.08070692067751395 2402 34-35 -0.053430152217011084 2402 36-37 -0.03329894909504105 2402 38-39 -0.4228766028771034 2402 40-41 0.18891996841256997 2402 42-43 0.05888333075835561 2402 44-45 0.22319325513099386 2402 46-47 -0.030749485120367126 2402 48-49 0.19986335167821068 2402 50-51 0.1369787350746563 2402 52-53 -0.07364349877612142 2402 54-55 0.0017675775034433627 2402 56-57 -0.18632321934902762 2402 58-59 0.09737215822954681 2402 60-61 -0.14961745980429697 2402 62-63 -0.14194849369416573 2402 64-65 0.12305407017008463 2402 66-67 -0.24721282435567815 2402 68-69 -0.3502016758227313 2402 70-71 -0.34117334574090563 2402 72-73 -0.5591163482208685 2402 74-75 -0.531245554388498 2402 76-77 -0.7706087350273236 2402 78-79 -0.8463126143178457 2402 80-81 -0.40130582854687447 2402 82-83 -5.189967980427923E-4 2402 84-85 -1.0581218332035007 2402 86-87 -0.39433941463612143 2402 88-89 -0.242286603664013 2402 90-91 -0.6966918498168511 2403 1 -0.13948051962192665 2403 2 0.12017776495288501 2403 3 0.11095796456852725 2403 4 -0.212410785774658 2403 5 0.09811976747082696 2403 6 -0.0788177237476333 2403 7 -0.038160338420638595 2403 8 -0.1548791836793839 2403 9 -0.08796442872834831 2403 10-11 -0.037233263579295794 2403 12-13 -0.02121833195035805 2403 14-15 0.04984477219001349 2403 16-17 0.20330832805995414 2403 18-19 0.11263471295945493 2403 20-21 0.0028399273264270164 2403 22-23 0.08658444245078556 2403 24-25 0.05940985199730875 2403 26-27 0.012316555831588971 2403 28-29 0.024648942290284026 2403 30-31 0.09191181387747349 2403 32-33 -0.07113051085439537 2403 34-35 -0.17735910577791714 2403 36-37 0.03395651331080529 2403 38-39 0.18975123093350277 2403 40-41 0.02323201374980499 2403 42-43 0.10895078729158314 2403 44-45 -0.167295959140084 2403 46-47 0.1906792250993874 2403 48-49 0.1802743079340985 2403 50-51 0.1343133666877705 2403 52-53 -0.05095413072789512 2403 54-55 0.09662164034916998 2403 56-57 0.033960659390182 2403 58-59 0.2894295837702643 2403 60-61 0.32277774933641723 2403 62-63 -0.10902027326594066 2403 64-65 0.2845985697482831 2403 66-67 0.37945162502639107 2403 68-69 0.07377341159103423 2403 70-71 0.024487518023860844 2403 72-73 0.30704347479412775 2403 74-75 0.2316518739619582 2403 76-77 0.1386118140377448 2403 78-79 0.22499814988874078 2403 80-81 0.17470915271036347 2403 82-83 0.7824653833947153 2403 84-85 0.8439589501502383 2403 86-87 0.01649894240912886 2403 88-89 0.6466022852248727 2403 90-91 -1.2205013736263837 2404 1 -0.12501530356308876 2404 2 -0.3956359034504189 2404 3 -0.2004425870710662 2404 4 0.05862813546347212 2404 5 0.0705377876976101 2404 6 0.11698368599800091 2404 7 0.10955648658701733 2404 8 0.012328907034678593 2404 9 0.14547106008562594 2404 10-11 0.08535331319055217 2404 12-13 0.013841429005815087 2404 14-15 0.008946825515181445 2404 16-17 0.040038331124620186 2404 18-19 0.09237728114823796 2404 20-21 0.025012774399662874 2404 22-23 -0.0460542336141927 2404 24-25 -0.07131340880563641 2404 26-27 0.030367429260948597 2404 28-29 0.08378164225963758 2404 30-31 0.12851923036535595 2404 32-33 0.18284872969782384 2404 34-35 0.0884789107067121 2404 36-37 0.08471421296971471 2404 38-39 0.3136155995376768 2404 40-41 0.13371562975699902 2404 42-43 0.09143998480784887 2404 44-45 -0.02891594029264155 2404 46-47 0.07365245628086825 2404 48-49 0.2708591510402769 2404 50-51 -0.02460549512812804 2404 52-53 0.05231963808375184 2404 54-55 0.056262663254457834 2404 56-57 0.25068056124709415 2404 58-59 0.05365773477215896 2404 60-61 0.11887246910738014 2404 62-63 0.23941864111430533 2404 64-65 0.09136303749048125 2404 66-67 -0.10398458082592299 2404 68-69 -0.3232290757741083 2404 70-71 -0.04056165417274116 2404 72-73 0.4413316563666996 2404 74-75 -0.21823577876966027 2404 76-77 0.46586105572684033 2404 78-79 0.47450228070145073 2404 80-81 0.20713059917686394 2404 82-83 0.7824653833947153 2404 84-85 -0.20366009746881275 2404 86-87 0.7973847232949112 2404 88-89 0.6466022852248727 2404 90-91 0.44616529304028774 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 7.0 31 148.0 32 960.0 33 1841.0 34 2332.0 35 2952.0 36 4338.0 37 6311.0 38 15382.0 39 79216.0 40 78390.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.84608368902995 15.529740406614653 27.33417762420717 28.28999828014822 2 27.341249569958613 18.273370794716485 28.295680820675344 26.08969881464955 3 28.723088228396314 20.356269902072682 20.9847975525988 29.935844316932204 4 28.243240426108528 22.711021701515563 20.573182680481146 28.472555191894767 5 27.9069403836833 22.470124089911767 21.23391547710252 28.389020049302417 6 27.82459596512349 24.430755119164882 22.95011908670659 24.79452982900504 7 26.503437097724063 24.71166424323916 22.939174575379017 25.84572408365776 8 25.38761810951808 25.021758730853623 23.36392584832992 26.226697311298388 9 25.728461462290948 24.739286105161117 23.327965311110763 26.20428712143717 10-11 25.54214418611923 24.110497871031964 23.58802774694205 26.759330195906756 12-13 26.227739645710535 23.98567832517707 22.84614622909468 26.94043580001772 14-15 26.31477456912501 23.80483330466914 22.68510556241759 27.19528656378826 16-17 26.48285099308411 23.83740625504881 22.296054243082807 27.38368850878427 18-19 26.58161217863527 24.017208941144588 22.116772724193105 27.284406156027035 20-21 26.58161217863527 24.08808768117075 22.023483794305726 27.30681634588825 22-23 26.642588741746014 23.75818883972545 22.365369481490745 27.23385293703779 24-25 26.990207268197857 23.823595324087826 22.183742710173703 27.002454697540614 26-27 26.8914460826467 23.814996065187593 22.275207554839817 27.018350297325895 28-29 26.88779791220417 23.887177723228945 22.37891982884869 26.846104535718197 30-31 27.06629768028477 24.00782793143524 22.3622424782543 26.56363191002569 32-33 26.998878684322943 23.780969671080733 22.467695429303717 26.752456215292607 34-35 26.956214181896556 23.702534968416515 22.82604490768117 26.51520594200576 36-37 27.181956266299828 23.826268879144813 22.65669331957699 26.33508153497836 38-39 27.468908929008972 23.662680555306547 22.713663337934992 26.15474717774949 40-41 27.201629225961256 23.685404225100967 22.98466187617507 26.12830467276271 42-43 27.579589855038954 23.66970416585472 22.865796261861274 25.884909717245048 44-45 27.699964825888145 23.796693633485756 22.86774533943018 25.63559620119592 46-47 27.51393191176581 23.49599105957533 23.24097894649996 25.7490980821589 48-49 28.19351505904684 23.45655281778974 23.08893667631759 25.26099544684583 50-51 28.195522638129322 23.565650360225284 23.04668536888186 25.19214163276353 52-53 28.127586573630936 23.47163843688876 23.217778517624538 25.182996471855766 54-55 28.630304849075948 23.58616550378873 22.87843549153475 24.905094155600572 56-57 28.633977389069205 23.39784782277213 23.047683359836192 24.920491428322478 58-59 28.09637180804009 23.456489601082936 23.449958736812107 24.997179854064868 60-61 28.54984306503074 23.38590438639724 22.983839589115718 25.080412959456304 62-63 27.916435570345723 23.288820626591008 23.461609568098755 25.333134234964515 64-65 27.7069788962694 23.37644004924988 23.331738724679052 25.584842329801667 66-67 27.868480725623584 23.399616256759113 23.01936159079016 25.71254142682714 68-69 28.01429171100286 23.17619610101819 23.1195759344768 25.689936253502154 70-71 27.648025771432984 23.35094822545591 23.154573436448484 25.84645256666262 72-73 28.321401160084047 23.230620179422978 22.43793801980447 26.0100406406885 74-75 27.93885387234659 23.4325871187423 22.74288198217779 25.885677026733315 76-77 27.962165357655604 23.499704416856684 22.542015032514147 25.99611519297357 78-79 28.688393000481614 23.579226200032107 22.138384973511 25.593995825975274 80-81 28.729551161713434 23.061169758880464 22.383402004047102 25.825877075359 82-83 28.91183478951549 22.869188000244396 21.983258996761776 26.235718213478343 84-85 29.178538464625387 22.665436012681088 21.481600385248203 26.674425137445322 86-87 30.274129069103367 21.399200456881783 20.79954311821816 27.527127355796686 88-89 29.875902944989814 21.522504167438413 20.92054084089646 27.68105204667531 90-91 28.74916685181071 19.462341701844036 20.417684958898022 31.370806487447233 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.5 8 1.5 9 1.5 10 1.0 11 0.5 12 1.0 13 1.5 14 2.0 15 6.0 16 12.0 17 17.0 18 23.0 19 24.5 20 34.5 21 63.0 22 101.0 23 155.0 24 228.5 25 287.0 26 339.0 27 438.0 28 586.5 29 768.5 30 962.5 31 1188.0 32 1460.5 33 1712.0 34 2084.5 35 2610.5 36 3186.5 37 3656.0 38 4100.5 39 4747.5 40 5489.0 41 6208.0 42 6925.0 43 7707.5 44 8436.0 45 9158.5 46 9983.5 47 10745.0 48 11390.0 49 11967.0 50 12407.5 51 12731.5 52 13077.5 53 13430.5 54 13797.5 55 14078.0 56 14146.0 57 14044.0 58 13762.5 59 13362.5 60 12936.0 61 12454.5 62 11947.5 63 11233.5 64 10277.0 65 9278.0 66 8159.5 67 7149.5 68 6225.0 69 5240.5 70 4360.0 71 3536.5 72 2832.0 73 2260.0 74 1722.5 75 1319.5 76 998.5 77 730.5 78 547.5 79 392.5 80 256.5 81 163.0 82 113.0 83 78.0 84 58.5 85 47.5 86 31.0 87 17.0 88 10.5 89 6.0 90 4.0 91 2.5 92 2.5 93 2.5 94 1.0 95 0.5 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01824085221261537 3 0.0 4 5.211672060747249E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.06599319762424488 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 8.721361230060789E-4 68-69 9.762002381928582E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.009260116677470136 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 3018.0 32-33 6360.0 34-35 6308.0 36-37 6867.0 38-39 7570.0 40-41 8132.0 42-43 9129.0 44-45 9048.0 46-47 8435.0 48-49 8519.0 50-51 8356.0 52-53 8041.0 54-55 8315.0 56-57 7673.0 58-59 7285.0 60-61 6825.0 62-63 6620.0 64-65 6465.0 66-67 6171.0 68-69 5981.0 70-71 5643.0 72-73 5340.0 74-75 4982.0 76-77 4725.0 78-79 4506.0 80-81 4212.0 82-83 3936.0 84-85 3793.0 86-87 3383.0 88-89 3208.0 90-91 3031.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.87613379034538 #Duplication Level Percentage of deduplicated Percentage of total 1 99.88145887512782 99.75773949787146 2 0.11411751868778809 0.22795233128567524 3 0.0033685089257674238 0.010093009444317595 4 0.0010550972586197043 0.004215161398549129 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.00104233441214945 0.0 0.0 0.0 0.0 2 0.00104233441214945 0.0 0.0 0.0 0.0 3 0.00104233441214945 0.0 0.0 0.0 0.0 4 0.00104233441214945 0.0 0.0 0.0 0.0 5 0.00104233441214945 0.0 0.0 0.0 0.0 6 0.0015635016182241749 0.0 0.0 0.0 0.0 7 0.0020846688242989 0.0 0.0 0.0 0.0 8 0.013029180151868124 0.0 0.0 0.0 0.0 9 0.013550347357942848 0.0 0.0 0.0 0.0 10-11 0.013550347357942848 0.0 0.0 0.0 0.0 12-13 0.013550347357942848 0.0 0.0 0.0 0.0 14-15 0.013550347357942848 0.0 0.0 0.0 0.0 16-17 0.013550347357942848 2.605836030373625E-4 0.0 0.0 0.0 18-19 0.013550347357942848 5.21167206074725E-4 0.0 0.0 0.0 20-21 0.013550347357942848 5.21167206074725E-4 0.0 0.0 0.0 22-23 0.013810930960980211 5.21167206074725E-4 0.0 0.0 0.0 24-25 0.014071514564017574 5.21167206074725E-4 0.0 0.0 0.0 26-27 0.014071514564017574 5.21167206074725E-4 0.0 0.0 0.0 28-29 0.014071514564017574 5.21167206074725E-4 0.0 0.0 0.0 30-31 0.014071514564017574 5.21167206074725E-4 0.0 0.0 0.0 32-33 0.01485326537312966 5.21167206074725E-4 0.0 0.0 0.0 34-35 0.015113848976167023 5.21167206074725E-4 0.0 0.0 0.0 36-37 0.015113848976167023 5.21167206074725E-4 0.0 0.0 0.0 38-39 0.015113848976167023 5.21167206074725E-4 0.0 0.0 0.0 40-41 0.015113848976167023 5.21167206074725E-4 0.0 0.0 0.0 42-43 0.015113848976167023 5.21167206074725E-4 0.0 0.0 0.0 44-45 0.015113848976167023 5.21167206074725E-4 0.0 0.0 0.0 46-47 0.015635016182241748 5.21167206074725E-4 0.0 0.0 0.0 48-49 0.015635016182241748 5.21167206074725E-4 0.0 0.0 0.0 50-51 0.01589559978527911 5.21167206074725E-4 0.0 0.0 0.0 52-53 0.016156183388316474 5.21167206074725E-4 0.0 0.0 0.0 54-55 0.01641676699135384 5.21167206074725E-4 0.0 0.0 0.0 56-57 0.0166773505943912 5.21167206074725E-4 0.0 0.0 0.0 58-59 0.0166773505943912 5.21167206074725E-4 0.0 0.0 0.0 60-61 0.01771968500654065 5.21167206074725E-4 0.0 0.0 0.0 62-63 0.01771968500654065 5.21167206074725E-4 0.0 0.0 0.0 64-65 0.01771968500654065 5.21167206074725E-4 0.0 0.0 0.0 66-67 0.018240852212615375 5.21167206074725E-4 0.0 0.0 0.0 68-69 0.018240852212615375 5.21167206074725E-4 0.0 0.0 0.0 70-71 0.018240852212615375 5.21167206074725E-4 0.0 0.0 0.0 72-73 0.018501435815652736 5.21167206074725E-4 0.0 0.0 0.0 74-75 0.019283186624764824 5.21167206074725E-4 0.0 0.0 0.0 76-77 0.019283186624764824 5.21167206074725E-4 0.0 0.0 0.0 78-79 0.019283186624764824 5.21167206074725E-4 0.0 0.0 0.0 80 0.019283186624764824 5.21167206074725E-4 0.0 0.0 0.0 >>END_MODULE