##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-28B_S23_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	932677
Sequences flagged as poor quality	0
Sequence length	61
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.48381272401914	40.0	40.0	40.0	40.0	40.0
2	39.22363261879514	40.0	40.0	40.0	40.0	40.0
3	39.173701077650676	40.0	40.0	40.0	40.0	40.0
4	39.04964741276991	40.0	40.0	40.0	40.0	40.0
5	39.06297678617571	40.0	40.0	40.0	40.0	40.0
6	39.18059092268813	40.0	40.0	40.0	40.0	40.0
7	39.22980410152711	40.0	40.0	40.0	40.0	40.0
8	39.21122103364831	40.0	40.0	40.0	40.0	40.0
9	39.230087157719126	40.0	40.0	40.0	40.0	40.0
10	39.2410577295248	40.0	40.0	40.0	40.0	40.0
11	39.22926372152417	40.0	40.0	40.0	40.0	40.0
12	39.248035493530985	40.0	40.0	40.0	40.0	40.0
13	39.07057212732811	40.0	40.0	40.0	40.0	40.0
14	39.24007561031311	40.0	40.0	40.0	40.0	40.0
15	39.15265413428229	40.0	40.0	40.0	40.0	40.0
16	39.33164428842997	40.0	40.0	40.0	40.0	40.0
17	39.28123884260039	40.0	40.0	40.0	40.0	40.0
18	39.3735108724671	40.0	40.0	40.0	40.0	40.0
19	39.3261675799875	40.0	40.0	40.0	40.0	40.0
20	39.330735077631374	40.0	40.0	40.0	40.0	40.0
21	39.313185593726445	40.0	40.0	40.0	40.0	40.0
22	39.374076984851136	40.0	40.0	40.0	40.0	40.0
23	39.35919937984961	40.0	40.0	40.0	40.0	40.0
24	39.36621145369726	40.0	40.0	40.0	40.0	40.0
25	39.32841916333307	40.0	40.0	40.0	40.0	40.0
26	39.29118869662273	40.0	40.0	40.0	40.0	40.0
27	39.309921870057906	40.0	40.0	40.0	40.0	40.0
28	39.322830947905864	40.0	40.0	40.0	40.0	40.0
29	39.32600460824058	40.0	40.0	40.0	40.0	40.0
30	39.327445621581745	40.0	40.0	40.0	40.0	40.0
31	39.27308167779413	40.0	40.0	40.0	40.0	40.0
32	39.29736446808488	40.0	40.0	40.0	40.0	40.0
33	39.30975460958081	40.0	40.0	40.0	40.0	40.0
34	39.304775393839456	40.0	40.0	40.0	40.0	40.0
35	39.30283688779717	40.0	40.0	40.0	40.0	40.0
36	39.272605628743925	40.0	40.0	40.0	40.0	40.0
37	39.29378766711305	40.0	40.0	40.0	40.0	40.0
38	39.27434685319784	40.0	40.0	40.0	40.0	40.0
39	38.048953710662964	40.0	40.0	40.0	40.0	40.0
40	39.272811487792666	40.0	40.0	40.0	40.0	40.0
41	39.25254294895232	40.0	40.0	40.0	40.0	40.0
42	39.23606993632308	40.0	40.0	40.0	40.0	40.0
43	39.270259693334346	40.0	40.0	40.0	40.0	40.0
44	39.22104651449537	40.0	40.0	40.0	40.0	40.0
45	39.067943135726516	40.0	40.0	40.0	40.0	40.0
46	39.089995786322596	40.0	40.0	40.0	40.0	40.0
47	39.17396912328705	40.0	40.0	40.0	40.0	40.0
48	39.25372663848256	40.0	40.0	40.0	40.0	40.0
49	39.24457877700426	40.0	40.0	40.0	40.0	40.0
50	39.22363261879514	40.0	40.0	40.0	40.0	40.0
51	39.18204480221985	40.0	40.0	40.0	40.0	40.0
52	39.19020625575628	40.0	40.0	40.0	40.0	40.0
53	39.11665024440401	40.0	40.0	40.0	40.0	40.0
54	39.02683994566179	40.0	40.0	40.0	40.0	40.0
55	39.084583408832856	40.0	40.0	40.0	40.0	40.0
56	39.08358413470044	40.0	40.0	40.0	40.0	40.0
57	39.006288350629426	40.0	40.0	40.0	40.0	40.0
58	38.98526928400722	40.0	40.0	40.0	40.0	40.0
59	38.94618608585824	40.0	40.0	40.0	40.0	40.0
60	38.89442111256094	40.0	40.0	40.0	40.0	40.0
61	38.768718430925176	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.01045363590058912
1101	2	0.031099222595379672
1101	3	0.030050113284126212
1101	4	-0.020359959268198224
1101	5	0.14595629060242032
1101	6	0.04796985756006222
1101	7	0.04622328250041363
1101	8	0.045655275127813866
1101	9	0.06655378990292604
1101	10	0.01731894755258878
1101	11	0.04061842525661774
1101	12	-0.003677367550615429
1101	13	0.15872792020411453
1101	14	0.07162242548637465
1101	15	0.1282222330903693
1101	16	0.07857461337069083
1101	17	0.14561899705952186
1101	18	0.08996312177168164
1101	19	0.06247350973238497
1101	20	0.09076593630558705
1101	21	0.03718934451868705
1101	22	0.043554144963870556
1101	23	0.030600869045208867
1101	24	0.021807704702219155
1101	25	-0.06717762492332469
1101	26	0.0560510796213336
1101	27	0.13646417294486923
1101	28	0.002977933886867845
1101	29	0.07852404029497961
1101	30	0.01077723984232648
1101	31	0.02523727609197124
1101	32	0.12185829335052745
1101	33	0.05275417724359244
1101	34	0.10705320419577902
1101	35	0.1015265986700058
1101	36	-0.1579167878986567
1101	37	-0.010406595436485588
1101	38	-0.04519967018840276
1101	39	1.236568218505603
1101	40	0.01364491241127297
1101	41	-0.08878784136968676
1101	42	0.01551834484639869
1101	43	-0.03590846609835552
1101	44	-0.10893977477177685
1101	45	0.14628281387444275
1101	46	0.09158571701848928
1101	47	0.10934454769903112
1101	48	0.012563966690528616
1101	49	0.008269626817480003
1101	50	0.09272166769961387
1101	51	-0.04741441929154888
1101	52	0.0856594439865006
1101	53	-0.023860350549576026
1101	54	0.024049699132312696
1101	55	0.019678013907274305
1101	56	0.21294589782949913
1101	57	0.11337055797655182
1101	58	0.09209696479388185
1101	59	0.004867950327678727
1101	60	0.1203312758583337
1101	61	0.1470042115424519
1102	1	-0.015021841431611449
1102	2	-0.17160422241295947
1102	3	0.08868254786523266
1102	4	-0.010442065680138057
1102	5	-0.20911213764548364
1102	6	-0.11486782951089225
1102	7	-0.006674351597162342
1102	8	-0.09341011399286003
1102	9	-0.13884752999504713
1102	10	-0.04086611622911818
1102	11	-0.008299105833401654
1102	12	-0.0949524590915587
1102	13	-0.1459646185998551
1102	14	-0.21338095456293615
1102	15	-0.12219885577537326
1102	16	-0.027583042784996792
1102	17	-0.1382725608751656
1102	18	-0.08528250009849359
1102	19	-0.05032381599418301
1102	20	-0.1719072121005354
1102	21	-0.03975799265083424
1102	22	0.09775206052857754
1102	23	-0.18558666650420008
1102	24	-0.07378301794582853
1102	25	6.008124866525577E-4
1102	26	-0.014238094302541526
1102	27	-0.20315675295636737
1102	28	-0.24092501527082533
1102	29	-0.146989150924
1102	30	0.008112829697537904
1102	31	-0.07809965433449406
1102	32	-0.16868878587626313
1102	33	-0.026802529371984463
1102	34	-0.06282572938407327
1102	35	-0.11070989536077036
1102	36	-0.09251258606969515
1102	37	-0.10973964527352109
1102	38	-0.07016789244098476
1102	39	1.2075749800352824
1102	40	-0.10689069523309058
1102	41	-0.14540930898148474
1102	42	-0.024062962759884954
1102	43	-0.11123672607362778
1102	44	-0.11114976890773676
1102	45	-0.07352023509405825
1102	46	-0.1454529965174629
1102	47	-0.1199162546160295
1102	48	-0.16096746765796865
1102	49	-0.18646172338480227
1102	50	-0.0345223003076498
1102	51	-0.16632302388104137
1102	52	-0.018942541859900075
1102	53	-0.17583256336289566
1102	54	-0.07616003895432044
1102	55	-0.1535390279700053
1102	56	-0.1409657950935994
1102	57	-0.2958742098821503
1102	58	-0.2308902216213724
1102	59	-0.1269659716292324
1102	60	-0.13754804784999664
1102	61	-0.15180645803095416
1103	1	-0.04494016299572934
1103	2	0.041253381408211
1103	3	0.02188490887908756
1103	4	-0.19622637402556364
1103	5	-0.1510179385485344
1103	6	-0.0013420473537095745
1103	7	-0.10522977813157297
1103	8	-0.12167598926478007
1103	9	-0.08362623433490768
1103	10	-0.054640160442154695
1103	11	-0.0962611399579103
1103	12	-0.05171227707661785
1103	13	-0.08924760441752255
1103	14	-0.09585532167029953
1103	15	-0.10398989858575902
1103	16	-0.0414308412427431
1103	17	-0.04204697824955872
1103	18	-0.08324020994139403
1103	19	-0.05887228416681012
1103	20	-0.07834907465862528
1103	21	-0.031097137057699342
1103	22	-0.04211715739106836
1103	23	-0.024484128348454703
1103	24	-0.04925051229901811
1103	25	-0.10071030091708622
1103	26	-0.026761389449028172
1103	27	-0.0618651317458756
1103	28	-0.008208422541329696
1103	29	-0.060071968599558545
1103	30	-0.11440745820743103
1103	31	-0.009746591009012207
1103	32	-0.04465521891465585
1103	33	-0.06447558295136702
1103	34	-0.14552531034582472
1103	35	-0.015849644289033904
1103	36	-0.08904701459503173
1103	37	-0.01091378650851027
1103	38	-0.044204203236105855
1103	39	1.2255718937713524
1103	40	-0.008038495666390588
1103	41	-0.1286025604680887
1103	42	-0.011344306667588455
1103	43	0.06445642114730532
1103	44	-0.13013226764008579
1103	45	0.02948854297810044
1103	46	-0.01196352076527063
1103	47	-0.06334411484295543
1103	48	-0.009064015476404563
1103	49	0.01712501577760861
1103	50	-0.07212686780265898
1103	51	-0.05142795904974662
1103	52	0.011031534401112708
1103	53	-0.08602930720079627
1103	54	3.744773736826801E-4
1103	55	0.07534234403455287
1103	56	0.07730769706013518
1103	57	-0.0892907655150168
1103	58	-0.08660452928632623
1103	59	0.006651257011121459
1103	60	-0.04491139945360345
1103	61	0.06943213036548457
1104	1	0.009862297055690306
1104	2	0.10985138829417451
1104	3	0.0021045693047838654
1104	4	0.0723116759203819
1104	5	0.025468039703447687
1104	6	0.08819183285959298
1104	7	0.02091780678471622
1104	8	0.006459259056882161
1104	9	0.02397830422376046
1104	10	0.03427779847825718
1104	11	-0.014908656741312143
1104	12	-0.0644449508836118
1104	13	-0.017915197691444007
1104	14	-0.020841922202642138
1104	15	-0.15947550437796565
1104	16	-0.0842982364770748
1104	17	-0.01741376028790853
1104	18	-0.0376075279584569
1104	19	-0.028895631035872782
1104	20	-0.014740211932767977
1104	21	-0.0029191921272371246
1104	22	0.0532743341740769
1104	23	-0.018633784504487494
1104	24	-0.04988096860767399
1104	25	0.05111702309567079
1104	26	-0.02431405617020488
1104	27	-0.008000180211070074
1104	28	0.020252955196475853
1104	29	-0.03033150947474894
1104	30	0.04550860207655205
1104	31	-0.10748367074265985
1104	32	0.14032829292683857
1104	33	0.02567058480088491
1104	34	-0.042132292604016186
1104	35	-0.04192546256499696
1104	36	-0.02084690376553766
1104	37	0.00733129202599514
1104	38	-0.039011568855343626
1104	39	1.1132198556896071
1104	40	0.032581422755832534
1104	41	-0.040058878203538484
1104	42	-0.09853078183542152
1104	43	-0.08493628772541228
1104	44	-0.10085692901964194
1104	45	-0.12150120075140336
1104	46	-0.07468938128362623
1104	47	0.0598237918422484
1104	48	-0.11176661996953641
1104	49	-0.06826638494369064
1104	50	0.012819398060948117
1104	51	0.06946885142273374
1104	52	0.03836281466347202
1104	53	-0.026288575544441528
1104	54	-0.05747670223368573
1104	55	0.1069323839150016
1104	56	0.03898612800518464
1104	57	0.02967652161513712
1104	58	-0.13144141915632446
1104	59	-0.10318077271298876
1104	60	-0.08222460179010938
1104	61	0.0184959845066075
1201	1	0.10861861458892008
1201	2	0.03417542932794504
1201	3	0.14938045353157037
1201	4	0.09758454322598453
1201	5	0.09253665871005268
1201	6	0.13142950882767224
1201	7	0.15096260487430158
1201	8	0.10653901150528355
1201	9	0.17395665713062414
1201	10	0.0821885121943069
1201	11	0.06296527970282284
1201	12	0.06251183240392066
1201	13	0.09733574908404563
1201	14	0.0408281396396859
1201	15	0.07216024112513963
1201	16	0.03983029712605202
1201	17	0.10024252033133507
1201	18	0.18812015616715883
1201	19	0.04100670639445525
1201	20	0.08658459016723441
1201	21	0.07152766767707419
1201	22	0.07923990846006745
1201	23	-0.0772486439806741
1201	24	0.17841884591091883
1201	25	0.13058053976848782
1201	26	0.10101577716996246
1201	27	0.14956695995727642
1201	28	0.13563879754183716
1201	29	0.15938634914923
1201	30	0.09560772297670184
1201	31	0.2044049128082861
1201	32	0.10439320686255371
1201	33	0.16283294711397645
1201	34	0.1148341976958065
1201	35	0.12790076824314411
1201	36	0.0929511812642474
1201	37	0.1368629400761634
1201	38	0.10072110093761921
1201	39	1.3931281631603625
1201	40	0.07187613767500522
1201	41	0.07570977589414696
1201	42	0.13752370503732436
1201	43	0.1472180765564275
1201	44	0.11136296898836662
1201	45	0.12740938080344932
1201	46	0.20958936035945896
1201	47	0.03335782790858133
1201	48	0.18374582369105497
1201	49	0.20204063919623394
1201	50	0.0340226118567557
1201	51	0.1768794489280765
1201	52	0.0894016472699164
1201	53	0.0768317959652478
1201	54	0.1752309161759129
1201	55	0.12967778219873338
1201	56	0.11304288440474863
1201	57	0.13433907761274355
1201	58	0.1276701841413228
1201	59	0.23043511215182377
1201	60	0.048239466313923174
1201	61	0.1798675443277773
1202	1	-0.024240324083969256
1202	2	0.015635876486072675
1202	3	0.0835638063195745
1202	4	0.12209293866460769
1202	5	0.04715639472513544
1202	6	0.18306819069891134
1202	7	0.04978185601913765
1202	8	0.15723040056876414
1202	9	0.058247758863586796
1202	10	0.03560505165736316
1202	11	0.0346946502694081
1202	12	0.004668607118837542
1202	13	0.08024407269004286
1202	14	0.02863904024128061
1202	15	0.07887210248816245
1202	16	0.12599353677766345
1202	17	0.042327462416274386
1202	18	-0.0032509491574330696
1202	19	-0.023799794458554402
1202	20	0.10769889383967524
1202	21	0.05991771885781816
1202	22	0.03751412952498612
1202	23	-0.00993249061800583
1202	24	-0.07367327943701696
1202	25	0.05236827434691804
1202	26	0.06636551228714183
1202	27	0.04031850884835819
1202	28	0.035670224317449595
1202	29	-0.011620205578260823
1202	30	0.15615814050386945
1202	31	0.021191300524904477
1202	32	0.03636321673954512
1202	33	0.061602813325698946
1202	34	0.06869520155680675
1202	35	0.06662753487689344
1202	36	-0.10834180607525212
1202	37	-0.041708488495267204
1202	38	-0.12482886763559975
1202	39	1.1911348076786368
1202	40	-0.10964042872527813
1202	41	-0.20499418387958457
1202	42	-0.14629150552207193
1202	43	-0.04815320834973846
1202	44	-0.09992822253538236
1202	45	-0.03522418299290564
1202	46	0.012781422853848312
1202	47	-0.09630455873055155
1202	48	-0.019149929204701266
1202	49	-0.06545060620198484
1202	50	0.07012299795714227
1202	51	-0.013849652266138435
1202	52	-0.016210276946658553
1202	53	-0.027491630451201843
1202	54	-0.04492711561002949
1202	55	0.019024105265984304
1202	56	-0.05195666664131693
1202	57	-0.09241408751250901
1202	58	0.08562564791073868
1202	59	0.00852167047094099
1202	60	-0.10494361710171773
1202	61	-0.004059904715951745
1203	1	0.021705621861976
1203	2	0.1514466872968825
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2403	6	-0.017560999992824122
2403	7	-0.05721906428964019
2403	8	-0.00382833015640216
2403	9	0.12235289944544547
2403	10	0.01774730509912814
2403	11	0.005217535906950843
2403	12	0.026038552463766962
2403	13	0.19447223413850168
2403	14	-0.05481136638493922
2403	15	0.12429685315961336
2403	16	0.001259768958419727
2403	17	0.013039105261341888
2403	18	0.042432051424803774
2403	19	0.15635628239661514
2403	20	-0.009970095511036448
2403	21	-0.029988935538064254
2403	22	-0.09053838442051187
2403	23	0.09613403455901448
2403	24	-0.024009473866335895
2403	25	0.006667431043574368
2403	26	-0.054887985825288865
2403	27	0.04106414677259096
2403	28	-0.05118344658764329
2403	29	-0.04391928714766635
2403	30	0.0233510109281454
2403	31	0.028774456996174536
2403	32	0.00379085980999605
2403	33	-0.06683097139717376
2403	34	0.041370482814436116
2403	35	0.08011287567286018
2403	36	0.024840020643750904
2403	37	0.007805062581866196
2403	38	0.05642031066013686
2403	39	1.1739006172686288
2403	40	-0.10876425720977778
2403	41	0.04510066426946935
2403	42	-0.012832647080585957
2403	43	0.0026769477271173514
2403	44	0.06541651761825307
2403	45	-0.055107547942284896
2403	46	-0.011125006160298767
2403	47	-0.021219571032638385
2403	48	-9.062164161619535E-4
2403	49	0.07093445850135538
2403	50	-0.055642830414662114
2403	51	0.06640221182867379
2403	52	-0.09428626815677177
2403	53	0.01161116240420057
2403	54	-0.0654650266969341
2403	55	-0.017776037475783824
2403	56	-0.06300037191712704
2403	57	-0.06511190694417479
2403	58	-0.0020563008900396085
2403	59	-0.12115640927663662
2403	60	0.013133876653327548
2403	61	0.06506467504101465
2404	1	-0.012027609117531313
2404	2	-0.056132020610888844
2404	3	-0.08395882542196631
2404	4	-0.03365884706639122
2404	5	0.013520056620492937
2404	6	-0.08141003882954578
2404	7	-0.08231683428876835
2404	8	-0.026306704253414637
2404	9	0.048211622970157464
2404	10	0.022832043110192046
2404	11	0.03256160277785369
2404	12	-0.011832202662191094
2404	13	0.023634761612427724
2404	14	0.023939969851184628
2404	15	0.06983017174550099
2404	16	0.004820337253228502
2404	17	0.016655999474288308
2404	18	-0.009748856833034836
2404	19	-0.02352222211656141
2404	20	0.07807261012609956
2404	21	0.03595475346777022
2404	22	0.004535914038974909
2404	23	-0.030969010338282033
2404	24	-0.028922345614383005
2404	25	0.02678059799720245
2404	26	-0.060809428500547824
2404	27	0.061120987808095606
2404	28	0.031499149883124744
2404	29	0.08027262247667721
2404	30	-0.06191776359559498
2404	31	0.012950725346328795
2404	32	0.08491029169811526
2404	33	-0.003428688560667581
2404	34	0.15699363161138535
2404	35	-0.04537501148658407
2404	36	0.018723603636502162
2404	37	0.04878337156708312
2404	38	-0.0038180923989870053
2404	39	1.3108438482051312
2404	40	-0.05901788030224964
2404	41	0.07646697939661351
2404	42	0.03674475207255057
2404	43	0.08122031134244878
2404	44	0.012222705670275502
2404	45	0.09213658615865228
2404	46	0.011114584557290641
2404	47	0.059021272816274006
2404	48	0.09289521257719002
2404	49	0.11741152506679242
2404	50	0.08689014612366464
2404	51	0.07570417242796879
2404	52	-0.004143135333272596
2404	53	0.07809211401373517
2404	54	0.029202954883047028
2404	55	-0.02455007161265854
2404	56	0.05922205624506205
2404	57	0.11693490470797485
2404	58	-0.004422470869627659
2404	59	0.10832741663968903
2404	60	0.07338046382021446
2404	61	0.05899254849929747
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	8.0
17	40.0
18	109.0
19	123.0
20	182.0
21	196.0
22	313.0
23	438.0
24	722.0
25	871.0
26	1174.0
27	1532.0
28	2099.0
29	2514.0
30	3187.0
31	4000.0
32	5636.0
33	6563.0
34	8332.0
35	10989.0
36	16028.0
37	23182.0
38	60785.0
39	405844.0
40	377810.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	26.573293862719893	16.606928229172585	26.91263963837427	29.907138269733252
2	26.631036920011113	16.90536022832211	29.69549544331792	26.768107408348857
3	28.2903173234864	19.885683774429705	21.515295772594126	30.308703129489768
4	28.01076900148712	22.232133954198506	21.52749558528837	28.22960145902601
5	27.49472754233245	21.782246158101895	21.362379473279603	29.360646826286057
6	29.095388864526516	23.207176761086636	22.731234929134096	24.96619944525275
7	27.519923832151967	23.86024314955767	22.873835207687122	25.745997810603242
8	25.35508005450976	25.08489005304087	23.26904169396265	26.29098819848672
9	25.659043806162263	24.559949478758455	23.397918035933127	26.383088679146155
10	26.442594810422044	23.347418238039534	23.461820115645608	26.74816683589281
11	25.57562800412147	23.998018606655894	23.316753817237906	27.109599571984727
12	27.550909907717248	22.894849985579143	22.53363168599633	27.020608420707276
13	26.136379475423965	22.666689539894307	23.019115942603925	28.177815042077803
14	26.092419991057998	23.39266434146012	23.080766438970834	27.43414922851105
15	26.89494862637333	22.46812133246558	22.341603792095228	28.295326249065862
16	26.473473667732772	23.792373994426796	22.516155110504492	27.21799722733594
17	26.333124972525322	22.614367031673343	22.751499179244263	28.301008816557072
18	27.977531342576263	22.800497921574138	22.142499493393746	27.079471242455856
19	26.32368976612482	23.001532148857535	23.455494238627093	27.219283846390553
20	26.171654281171296	22.78227081830044	22.742600064116516	28.303474836411745
21	26.991337837214814	22.713543917133155	22.984270009874802	27.31084823577723
22	27.358989232070698	22.953069497800417	22.457292288755916	27.230648981372973
23	26.273940496013086	23.785940899153726	22.694030194804846	27.246088410028335
24	27.011387650815877	23.854775018575562	22.094894588373037	27.038942742235523
25	26.45556821922273	23.085591260425637	22.53834928919658	27.920491231155054
26	26.48055007253315	22.84467184244921	23.55241954074133	27.122358544276317
27	27.01267426987049	23.140594225010375	22.009227202986672	27.837504302132466
28	26.526546703735594	23.42654530989828	22.44249616962786	27.60441181673827
29	26.588411636611603	24.270031318452155	22.64095715880203	26.500599886134214
30	28.211588792261416	23.39384374226018	21.945003468510535	26.449563996967868
31	26.760496935166195	23.556493834414272	23.3395913054573	26.343417924962232
32	26.696058764180954	23.485729786410513	22.50157342788554	27.316638021522994
33	27.26892589824773	24.529177839702278	21.994967175131368	26.206929086918624
34	27.0554543534364	23.95395190403537	22.12513013615646	26.865463606371765
35	27.793866472530148	23.96681809458151	22.216801743797692	26.022513689090648
36	27.43822352218399	25.058514362421285	21.725634919698887	25.77762719569583
37	27.037120031908152	25.36526578869212	22.003544635495462	25.59406954390427
38	27.76684747238326	24.396119985804305	22.001078615640786	25.835953926171655
39	27.89909701027769	24.365678204684308	22.08071486962899	25.65450991540901
40	28.27205988782826	24.848581020010144	21.46873998179434	25.410619110367254
41	27.26796093395677	24.87978153208453	21.380821013062402	26.4714365208963
42	27.818848325840563	25.02002300903743	21.697436518751935	25.46369214637007
43	27.447873165093593	26.36775646874534	21.046300058862823	25.13807030729824
44	27.295194370612762	25.449860991532976	22.017911881605315	25.23703275624895
45	27.939254425701503	25.505078392626817	20.37296942028162	26.18269776139006
46	27.497300780441673	25.709972477074057	21.53575139088881	25.25697535159546
47	28.196363800115154	25.84914177148144	20.63929956458667	25.315194863816732
48	28.831953613094353	25.834452870607937	20.0953813592487	25.238212157049013
49	28.36802022565154	26.275763206340457	20.23648058223801	25.119735985769992
50	28.317413209503396	26.201675392445615	20.239697129874543	25.241214268176442
51	28.797429335128882	26.2284799560834	19.678731222062943	25.295359486724774
52	28.389142221798114	26.62915457334104	19.897027588329077	25.084675616531772
53	28.19518439931509	26.572972207956237	19.938413834585823	25.29342955814285
54	28.65054032639381	26.483659401915133	19.497103498853303	25.368696772837758
55	28.1214182401839	26.95949401561312	19.683341607008643	25.235746137194333
56	27.904730147736036	26.905563233573897	19.601534078786116	25.58817253990395
57	28.15326206178559	26.961316725940492	19.255540771349565	25.629880440924353
58	27.857125242715323	27.23579545759143	19.37894898233794	25.528130317355313
59	27.693081313252073	27.057062627254663	19.409506184885014	25.840349874608254
60	28.040254021488682	27.03594063110809	19.034778385228755	25.889026962174473
61	28.091504347164133	27.1818646755522	19.0671582981032	25.659472679180467
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	3.0
2	2.0
3	2.0
4	5.0
5	8.0
6	6.0
7	8.0
8	12.0
9	10.0
10	8.0
11	9.5
12	13.0
13	15.0
14	22.0
15	29.0
16	33.5
17	61.5
18	85.0
19	115.0
20	187.0
21	229.0
22	274.0
23	319.0
24	442.0
25	776.0
26	987.0
27	1275.5
28	1564.0
29	1994.5
30	3003.0
31	3581.0
32	4421.0
33	5261.0
34	6252.5
35	8671.0
36	10098.0
37	11723.5
38	13349.0
39	15447.0
40	20020.0
41	22495.0
42	25464.5
43	31620.5
44	34807.0
45	38464.5
46	42122.0
47	45401.0
48	51693.0
49	54706.0
50	57741.0
51	60776.0
52	63146.5
53	67355.5
54	69194.0
55	69971.0
56	70748.0
57	69373.0
58	65255.5
59	62513.0
60	59423.0
61	55667.0
62	55001.0
63	46500.5
64	38000.0
65	33763.5
66	25530.5
67	21534.0
68	18377.5
69	15221.0
70	12724.0
71	8294.5
72	6362.0
73	5155.0
74	3948.0
75	3161.5
76	1853.0
77	1331.0
78	987.0
79	643.0
80	491.0
81	256.0
82	173.0
83	121.5
84	51.0
85	32.0
86	27.5
87	23.0
88	11.5
89	1.0
90	2.0
91	1.0
92	0.0
93	3.0
94	5.5
95	5.0
96	4.0
97	3.0
98	2.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.033237658910855526
3	1.0721825455114686E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.075019540526892
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	932677.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.41428782321947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.6823523260244	62.45966845221255
2	14.682063470444753	22.732029746795735
3	3.148173190901528	7.311407565533824
4	0.7755110457655499	2.4014254122792082
5	0.2768944752002148	1.0717794299904368
6	0.12536521955385177	0.582303551373779
7	0.08285876735139773	0.44901167250928
8	0.05297867115205418	0.3281044877645453
9	0.03730841417254498	0.259938388168315
>10	0.13514688517935597	1.4658133798119117
>50	0.0011978082261076867	0.06108422137166132
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	1.4972602826346084E-4	0.8774336921887594
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGTGCTATCTGGGGGGGGGGGGGGG	7570	0.8116421869521818	TruSeq Adapter, Index 7 (97% over 35bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.938159727322535	0.0	0.0	0.0	0.0
2	0.9416979297227229	0.0	0.0	0.0	0.0
3	0.9434134217955412	0.0	0.0	0.0	0.0
4	0.9463083146684221	0.0	0.0	0.0	0.0
5	0.9497392988140589	0.0	0.0	0.0	0.0
6	0.9546713385234117	0.0	0.0	0.0	0.0
7	0.9638921084148103	0.0	0.0	0.0	0.0
8	1.0152496523448096	0.0	0.0	0.0	0.0
9	1.0231838031815945	0.0	0.0	0.0	0.0
10	1.0326190095820955	0.0	0.0	0.0	0.0
11	1.0477367834738072	0.0	0.0	0.0	0.0
12	1.0677865970748717	0.0	0.0	0.0	0.0
13	1.0969499623127836	0.0	0.0	0.0	0.0
14	1.1302948394781902	0.0	0.0	0.0	0.0
15	1.1765059071897346	0.0	0.0	0.0	0.0
16	1.2276490146106316	0.0	0.0	0.0	0.0
17	1.283402506977228	0.0	0.0	0.0	0.0
18	1.3407642731620915	0.0	0.0	0.0	0.0
19	1.4217140553482073	0.0	0.0	0.0	0.0
20	1.5119918256802731	0.0	0.0	0.0	0.0
21	1.6276803223409604	0.0	0.0	0.0	0.0
22	1.7716744382031506	0.0	0.0	0.0	0.0
23	1.9585558558857996	0.0	1.0721825455114686E-4	0.0	0.0
24	2.174278984042707	0.0	1.0721825455114686E-4	0.0	0.0
25	2.4381431084930796	0.0	1.0721825455114686E-4	0.0	1.0721825455114686E-4
26	2.7322427807268754	0.0	1.0721825455114686E-4	0.0	1.0721825455114686E-4
27	3.094747699364303	0.0	1.0721825455114686E-4	0.0	1.0721825455114686E-4
28	3.4806261974938804	0.0	1.0721825455114686E-4	0.0	1.0721825455114686E-4
29	3.9272974459539585	0.0	1.0721825455114686E-4	0.0	1.0721825455114686E-4
30	4.434547008235434	0.0	1.0721825455114686E-4	0.0	2.1443650910229372E-4
31	5.001302701792796	0.0	1.0721825455114686E-4	0.0	2.1443650910229372E-4
32	5.63078107426258	0.0	1.0721825455114686E-4	0.0	2.1443650910229372E-4
33	6.314297447026141	0.0	1.0721825455114686E-4	0.0	2.1443650910229372E-4
34	7.100100034631496	0.0	1.0721825455114686E-4	0.0	2.1443650910229372E-4
35	7.929218797075515	0.0	1.0721825455114686E-4	0.0	2.1443650910229372E-4
36	8.78417715886636	0.0	1.0721825455114686E-4	0.0	2.1443650910229372E-4
37	9.69649728684207	0.0	1.0721825455114686E-4	0.0	2.1443650910229372E-4
38	10.635836414964666	0.0	1.0721825455114686E-4	0.0	2.1443650910229372E-4
39	11.632322872763025	0.0	1.0721825455114686E-4	0.0	2.1443650910229372E-4
40	12.746642192313095	0.0	1.0721825455114686E-4	0.0	2.1443650910229372E-4
41	13.902883849392662	0.0	1.0721825455114686E-4	0.0	2.1443650910229372E-4
42	15.124957514766635	0.0	1.0721825455114686E-4	0.0	2.1443650910229372E-4
43	16.407395057452902	0.0	1.0721825455114686E-4	0.0	3.216547636534406E-4
44	17.70130495337614	0.0	1.0721825455114686E-4	0.0	3.216547636534406E-4
45	19.039710424938107	0.0	1.0721825455114686E-4	0.0	3.216547636534406E-4
46	20.360746539262788	0.0	1.0721825455114686E-4	0.0	4.2887301820458744E-4
47	21.681568217078368	0.0	1.0721825455114686E-4	0.0	4.2887301820458744E-4
48	23.010752918748935	0.0	1.0721825455114686E-4	0.0	4.2887301820458744E-4
49	24.400837589004553	0.0	2.1443650910229372E-4	0.0	4.2887301820458744E-4
50	25.805289505370027	0.0	2.1443650910229372E-4	0.0	4.2887301820458744E-4
>>END_MODULE