##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-28B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 493452 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.580733283075155 40.0 40.0 40.0 40.0 40.0 2 39.36151844556309 40.0 40.0 40.0 40.0 40.0 3 39.32014461386315 40.0 40.0 40.0 40.0 40.0 4 39.20446973565818 40.0 40.0 40.0 40.0 40.0 5 39.22680220163258 40.0 40.0 40.0 40.0 40.0 6 39.34492513962858 40.0 40.0 40.0 40.0 40.0 7 39.39055470440894 40.0 40.0 40.0 40.0 40.0 8 39.38019503416746 40.0 40.0 40.0 40.0 40.0 9 39.395385974724995 40.0 40.0 40.0 40.0 40.0 10-11 39.39928098376336 40.0 40.0 40.0 40.0 40.0 12-13 39.32006760535979 40.0 40.0 40.0 40.0 40.0 14-15 39.35905012037645 40.0 40.0 40.0 40.0 40.0 16-17 39.46194158702366 40.0 40.0 40.0 40.0 40.0 18-19 39.503092499371775 40.0 40.0 40.0 40.0 40.0 20-21 39.48107212048994 40.0 40.0 40.0 40.0 40.0 22-23 39.52524257678559 40.0 40.0 40.0 40.0 40.0 24-25 39.50338026798959 40.0 40.0 40.0 40.0 40.0 26-27 39.477663480946475 40.0 40.0 40.0 40.0 40.0 28-29 39.4957969569482 40.0 40.0 40.0 40.0 40.0 30-31 39.47820087395252 40.0 40.0 40.0 40.0 40.0 32-33 39.4870229220971 40.0 40.0 40.0 40.0 40.0 34-35 39.4847941989184 40.0 40.0 40.0 40.0 40.0 36-37 39.47534695136321 40.0 40.0 40.0 40.0 40.0 38-39 39.40728285470281 40.0 40.0 40.0 40.0 40.0 40-41 39.46077906400225 40.0 40.0 40.0 40.0 40.0 42-43 39.44098765997698 40.0 40.0 40.0 40.0 40.0 44-45 39.35113846420079 40.0 40.0 40.0 40.0 40.0 46-47 39.338821449065335 40.0 40.0 40.0 40.0 40.0 48-49 39.42272064577696 40.0 40.0 40.0 40.0 40.0 50-51 39.379847717121045 40.0 40.0 40.0 40.0 40.0 52-53 39.34357016638565 40.0 40.0 40.0 40.0 40.0 54-55 39.255971716753734 40.0 40.0 40.0 40.0 40.0 56-57 39.27975275416287 40.0 40.0 40.0 40.0 40.0 58-59 39.22689407267477 40.0 40.0 40.0 40.0 40.0 60-61 39.14770742436741 40.0 40.0 40.0 40.0 40.0 62-63 39.39802449921204 40.0 40.0 40.0 40.0 40.0 64-65 39.399712645678804 40.0 40.0 40.0 40.0 40.0 66-67 39.39487766607183 40.0 40.0 40.0 40.0 40.0 68-69 39.398851512814744 40.0 40.0 40.0 40.0 40.0 70-71 39.38581002873951 40.0 40.0 40.0 40.0 40.0 72-73 39.39122972000611 40.0 40.0 40.0 40.0 40.0 74-75 39.38835452297615 40.0 40.0 40.0 40.0 40.0 76-77 39.37918893364271 40.0 40.0 40.0 40.0 40.0 78-79 39.38151902464317 40.0 40.0 40.0 40.0 40.0 80-81 39.38442065049168 40.0 40.0 40.0 40.0 40.0 82-83 39.36943603727084 40.0 40.0 40.0 40.0 40.0 84-85 39.35039861658673 40.0 40.0 40.0 40.0 40.0 86-87 39.345883653829475 40.0 40.0 40.0 40.0 40.0 88-89 39.335488409059906 40.0 40.0 40.0 40.0 40.0 90-91 39.47704064199127 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06499747168819425 1101 2 -0.1957118546768868 1101 3 -0.22793242341746378 1101 4 -0.07510527721324678 1101 5 -0.11532582639328126 1101 6 -0.07780429110904663 1101 7 -0.1832841469830555 1101 8 0.03736119450974229 1101 9 0.19263138030814275 1101 10-11 -0.040230416012050796 1101 12-13 -0.09802291131057927 1101 14-15 0.0066678124878833955 1101 16-17 -0.02119815113004364 1101 18-19 -0.07480518865909147 1101 20-21 -0.12940271188497832 1101 22-23 -0.021050096044689326 1101 24-25 0.043883822858646226 1101 26-27 -0.04114275432484504 1101 28-29 -0.09739973490740539 1101 30-31 0.024844117000611732 1101 32-33 0.06390422948063446 1101 34-35 0.07302304097898826 1101 36-37 -0.11459113872650306 1101 38-39 -0.02686959271315459 1101 40-41 -0.18649680723098783 1101 42-43 -0.11738566973414777 1101 44-45 -0.11538860137457618 1101 46-47 -0.1443633381416447 1101 48-49 -0.061490089950311244 1101 50-51 -0.14387378124791184 1101 52-53 -0.08643703589328311 1101 54-55 -0.07022454298625291 1101 56-57 -0.07218428599122007 1101 58-59 -0.015429266580312628 1101 60-61 0.08030083433330049 1101 62-63 -0.04111583343511427 1101 64-65 -0.055831615490490094 1101 66-67 0.05555793358467298 1101 68-69 -0.0036142438803565824 1101 70-71 0.09921891542516903 1101 72-73 0.06843392783837743 1101 74-75 -0.07368277198766293 1101 76-77 0.19872217738074482 1101 78-79 0.031049542039177425 1101 80-81 -0.05315767316744768 1101 82-83 -0.1135816203722868 1101 84-85 -0.3303111771293601 1101 86-87 -0.5093114681678728 1101 88-89 0.27261322688319467 1101 90-91 -0.5526922639552581 1102 1 0.051640704113061986 1102 2 -0.028199124174868473 1102 3 -0.016181833922061628 1102 4 0.028795052587817338 1102 5 -0.14263638285208202 1102 6 -0.02071108248021858 1102 7 -0.04173971987542302 1102 8 -0.2869307613495664 1102 9 0.057414072022666574 1102 10-11 -0.061805891195625406 1102 12-13 -0.13895372087672797 1102 14-15 -0.0105419507083937 1102 16-17 -0.04846464372562309 1102 18-19 -0.07057053841882777 1102 20-21 -0.010459657084226137 1102 22-23 0.07789474390744999 1102 24-25 0.034767122541502715 1102 26-27 -0.07570464089089768 1102 28-29 -0.04469596496144135 1102 30-31 -0.08984169070694747 1102 32-33 0.10482604729350697 1102 34-35 -0.02492116412118861 1102 36-37 0.027808482316963534 1102 38-39 8.014207020252684E-4 1102 40-41 0.004964384949914802 1102 42-43 -0.04174659534343306 1102 44-45 -0.06521825973270268 1102 46-47 -0.0716511367160848 1102 48-49 -0.10591447848970148 1102 50-51 -0.11054888164728993 1102 52-53 -0.0664201737782264 1102 54-55 -0.057346337888319 1102 56-57 -0.080706344464609 1102 58-59 0.057565375496800186 1102 60-61 -0.06999707052446524 1102 62-63 0.08355019461291135 1102 64-65 -0.10946324662202755 1102 66-67 -6.043567138007688E-4 1102 68-69 -0.23386344257922076 1102 70-71 0.020757220443016422 1102 72-73 -0.09612037687764996 1102 74-75 -0.13784082113139817 1102 76-77 -0.17115266412924512 1102 78-79 -0.12363599071853315 1102 80-81 -0.09263762116224683 1102 82-83 -0.02926119438862429 1102 84-85 -0.2050151828011053 1102 86-87 -0.29115586191780807 1102 88-89 -0.22009117563252545 1102 90-91 0.25726248717596434 1103 1 0.07121337875724976 1103 2 0.05000682038389925 1103 3 0.0933511769424129 1103 4 -0.09637672564503674 1103 5 0.08494686733242673 1103 6 0.09436065849389053 1103 7 -0.0153757167780455 1103 8 0.09050859962715663 1103 9 -0.0031707417083595146 1103 10-11 0.01789370534223167 1103 12-13 0.07969513497155134 1103 14-15 -0.06366620945470913 1103 16-17 -0.01564282473928813 1103 18-19 0.051878104223554544 1103 20-21 0.02328124768142459 1103 22-23 -0.061231728349639525 1103 24-25 0.05865454719160823 1103 26-27 -0.027867051603372772 1103 28-29 0.008541666042674478 1103 30-31 0.04206417949941965 1103 32-33 0.0052809053711371234 1103 34-35 0.005982537117532161 1103 36-37 0.06267355427532806 1103 38-39 0.0875658546812943 1103 40-41 0.14726790299162928 1103 42-43 0.13993109408865934 1103 44-45 0.1100177433219045 1103 46-47 0.028917024988750484 1103 48-49 0.0627445951011083 1103 50-51 -0.03985402326363641 1103 52-53 0.05359881441431469 1103 54-55 0.16260117722207923 1103 56-57 0.10570068121140963 1103 58-59 0.12334463590875089 1103 60-61 0.3056458995009521 1103 62-63 -0.003069405843156403 1103 64-65 0.06276519882020892 1103 66-67 0.1507331752982637 1103 68-69 0.06718882081573696 1103 70-71 -0.011802040324603524 1103 72-73 -0.0026937552439605383 1103 74-75 0.027340783062868468 1103 76-77 0.039525867572642426 1103 78-79 -0.014770342349905263 1103 80-81 0.44561703038986167 1103 82-83 0.017798799756974404 1103 84-85 0.14548727378358706 1103 86-87 0.5416632124159975 1103 88-89 0.5459465602165281 1103 90-91 -0.03397568641514681 1104 1 0.09136853977827997 1104 2 0.04765194053443622 1104 3 0.10133275123125429 1104 4 -0.06820083297969859 1104 5 -0.028274450584511612 1104 6 -0.1160600927290929 1104 7 -0.12847956262859128 1104 8 0.0218418260028983 1104 9 -0.2874574931978344 1104 10-11 7.786778912972636E-4 1104 12-13 -0.12045007355497006 1104 14-15 0.026791632485540617 1104 16-17 -0.07254716386374582 1104 18-19 -0.08899592058154582 1104 20-21 -0.021751886511374607 1104 22-23 -0.09154822311370481 1104 24-25 -0.04679729877858563 1104 26-27 -0.0021232657941041566 1104 28-29 0.024016435381376766 1104 30-31 -0.07081995007879271 1104 32-33 -0.04551762453654362 1104 34-35 0.007601472036263601 1104 36-37 -0.02813104656240739 1104 38-39 -0.06600754086992566 1104 40-41 -0.17204088093781422 1104 42-43 -0.05678084318880394 1104 44-45 -0.10374011753805235 1104 46-47 -0.18946282039960494 1104 48-49 -0.06831602460655972 1104 50-51 0.05420667224459663 1104 52-53 0.08172949232606896 1104 54-55 -0.15068736549400086 1104 56-57 0.06017831392234285 1104 58-59 -0.06575087045383299 1104 60-61 -0.03372604554892433 1104 62-63 0.060240129465633174 1104 64-65 -0.2004425737592186 1104 66-67 -0.01177182409588795 1104 68-69 -0.05703713728697579 1104 70-71 -0.051325915311409176 1104 72-73 -0.32158710234437393 1104 74-75 0.10082897616543107 1104 76-77 -0.06422057909649226 1104 78-79 0.015495222458845603 1104 80-81 0.28569406394516506 1104 82-83 0.17815222327226365 1104 84-85 0.30290363246988505 1104 86-87 0.16391660185031043 1104 88-89 0.41896243323240157 1104 90-91 0.5649547948682709 1201 1 0.060665712720876286 1201 2 0.08607885065932663 1201 3 -0.13281070600812228 1201 4 0.12399714435331788 1201 5 -0.06412881641149681 1201 6 0.07466724498856792 1201 7 0.11430067726412574 1201 8 -0.011204564184239985 1201 9 -0.051482186613014846 1201 10-11 0.04522575018430075 1201 12-13 -0.008014944833689697 1201 14-15 -0.0014397029960591112 1201 16-17 0.11607096703716024 1201 18-19 0.06868395192002907 1201 20-21 0.07292376381396082 1201 22-23 -0.07096894375936813 1201 24-25 -0.0017256346606302486 1201 26-27 0.11119876181800947 1201 28-29 0.08738616740353677 1201 30-31 0.07240938316137147 1201 32-33 0.0941665432255192 1201 34-35 0.011165890100357956 1201 36-37 0.10061922098703491 1201 38-39 0.207724982329907 1201 40-41 0.0032978198975044393 1201 42-43 0.145065524942666 1201 44-45 0.09657762765030498 1201 46-47 0.12654468035584898 1201 48-49 0.1382057661466547 1201 50-51 0.07373271614761734 1201 52-53 -0.057096312786512726 1201 54-55 0.038512918386892636 1201 56-57 0.10591774263736653 1201 58-59 -0.061300454962427864 1201 60-61 -0.14588052869699197 1201 62-63 0.015163376606729173 1201 64-65 0.06645632742283425 1201 66-67 -0.1311664523611853 1201 68-69 0.05512577069286806 1201 70-71 -0.07760815023458179 1201 72-73 -0.10210984920128396 1201 74-75 0.21415969069756358 1201 76-77 -0.2797694544128362 1201 78-79 0.08165360534059829 1201 80-81 -0.3315461724355444 1201 82-83 0.20306909722506816 1201 84-85 0.12062065526588128 1201 86-87 0.37481115457395475 1201 88-89 0.2767157909857545 1201 90-91 0.31495479486827094 1202 1 0.08397234054316272 1202 2 0.1589120626039744 1202 3 -0.007537581463331833 1202 4 0.21431032716467513 1202 5 0.13106419160016003 1202 6 0.19994036953335836 1202 7 -0.07506567379196127 1202 8 0.0674553047159776 1202 9 0.04465765315391934 1202 10-11 0.06270572105102445 1202 12-13 0.10414820156106686 1202 14-15 0.05682686655965341 1202 16-17 0.01410447031464912 1202 18-19 0.049747316814411136 1202 20-21 9.055186931306025E-5 1202 22-23 0.0033209324241738614 1202 24-25 -0.06290553269413834 1202 26-27 -0.0504909686991013 1202 28-29 0.03931624752006968 1202 30-31 -0.01207714269442306 1202 32-33 -0.03147054901415913 1202 34-35 -0.06151526722889855 1202 36-37 -0.3075006743902762 1202 38-39 -0.07762854883966241 1202 40-41 -0.11084935726634626 1202 42-43 -0.10501369442367547 1202 44-45 -0.16656759633177387 1202 46-47 -0.13353972157475624 1202 48-49 -0.11713873388735152 1202 50-51 -0.052206023833747395 1202 52-53 -0.034788291373793356 1202 54-55 -0.013066307559668644 1202 56-57 -0.20255443432796483 1202 58-59 0.03852095365440533 1202 60-61 -0.05299457014053388 1202 62-63 -0.07561225530115223 1202 64-65 -0.06269580475485981 1202 66-67 -0.035464029035154 1202 68-69 -0.005512569325006211 1202 70-71 -0.01874294849814362 1202 72-73 -0.17014703913960005 1202 74-75 -0.07568126573321621 1202 76-77 -0.2918813731390273 1202 78-79 -0.45097485760857836 1202 80-81 -0.431403593913366 1202 82-83 -0.34648520725534127 1202 84-85 -0.3886309280072524 1202 86-87 -0.09395270910824394 1202 88-89 -0.2968762144426549 1202 90-91 0.5649547948682709 1203 1 -0.05043089117499733 1203 2 0.06948399470810784 1203 3 0.04364597133969994 1203 4 -0.0869118969801903 1203 5 -0.1752128357351026 1203 6 -0.08856094948712467 1203 7 0.08548200679271645 1203 8 0.036293806375830684 1203 9 0.04818762567235524 1203 10-11 -0.04470255445882998 1203 12-13 -0.11098296376508188 1203 14-15 -0.06303698934279822 1203 16-17 -0.012989065043015557 1203 18-19 -0.05456620263425549 1203 20-21 -0.07872138061236456 1203 22-23 0.03182918344653274 1203 24-25 -0.10679108230173995 1203 26-27 0.08147400214401301 1203 28-29 0.08234080264232801 1203 30-31 0.13805260948913656 1203 32-33 -0.05791073249024237 1203 34-35 -0.06045535333647223 1203 36-37 0.07140500885152079 1203 38-39 0.06341893780483332 1203 40-41 0.019968118910597354 1203 42-43 -0.039019490601639006 1203 44-45 -0.14143544916763062 1203 46-47 0.0042844895025524465 1203 48-49 -0.06345691538648168 1203 50-51 -0.04439626422342968 1203 52-53 -0.01705391733695194 1203 54-55 0.03285942209107873 1203 56-57 -0.287031765651669 1203 58-59 0.11531596396050503 1203 60-61 0.05259852760079298 1203 62-63 0.017121719440858385 1203 64-65 0.06313336457492369 1203 66-67 0.12533171024349343 1203 68-69 -0.08424247785691108 1203 70-71 -0.17988461783438936 1203 72-73 -0.08628561503123677 1203 74-75 -0.03171894231041961 1203 76-77 1.738902248646923E-4 1203 78-79 -0.008236133601826623 1203 80-81 -0.008369520143993725 1203 82-83 -0.39346532073791707 1203 84-85 0.248614383740545 1203 86-87 0.17352467539700456 1203 88-89 0.0844080986780682 1203 90-91 0.5649547948682709 1204 1 -0.01586087571114092 1204 2 -0.14450291000814985 1204 3 -0.04493463451952806 1204 4 -0.3482658999025574 1204 5 -0.16914870548090732 1204 6 0.015366357630085759 1204 7 -0.16858524179188805 1204 8 -0.0889357018907262 1204 9 -0.1977131756363164 1204 10-11 -0.154426675937394 1204 12-13 -0.06748821849263464 1204 14-15 -0.0160138872856308 1204 16-17 -0.017686795733439453 1204 18-19 -0.14798192464276383 1204 20-21 -0.14280583965445715 1204 22-23 -0.03068226534170293 1204 24-25 -0.037660636406045 1204 26-27 0.03367664250083635 1204 28-29 -0.08508201654988312 1204 30-31 -0.08396761705841982 1204 32-33 -0.1097416492316583 1204 34-35 -0.05648909029144278 1204 36-37 -0.1009447276647748 1204 38-39 -0.021373157982928603 1204 40-41 -0.03918479910801409 1204 42-43 -0.05869098935816908 1204 44-45 -0.003188169124186402 1204 46-47 0.12840362260487836 1204 48-49 0.11786021687566262 1204 50-51 0.00783370416574769 1204 52-53 0.012039444521846576 1204 54-55 -0.0834154420526545 1204 56-57 0.12805073907886566 1204 58-59 0.01445906462275559 1204 60-61 0.09213188544001838 1204 62-63 -0.04183416288168473 1204 64-65 -0.10170436126099958 1204 66-67 -0.07102824070376101 1204 68-69 -0.037190575402036075 1204 70-71 -0.08299682478568116 1204 72-73 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-0.03602896592652627 2401 38-39 0.02268941146091663 2401 40-41 -0.031650838688570104 2401 42-43 0.0013211080581783108 2401 44-45 0.03213225401869124 2401 46-47 0.03913531915688395 2401 48-49 0.04881150224403541 2401 50-51 0.14539982936791063 2401 52-53 0.059782521026356505 2401 54-55 0.12895124993213614 2401 56-57 0.12491571001633162 2401 58-59 -0.11890992561864522 2401 60-61 0.06914784048040445 2401 62-63 0.07008004014154068 2401 64-65 -0.07601740624820508 2401 66-67 -0.05609362656286976 2401 68-69 0.1288243674743228 2401 70-71 0.15110274585550343 2401 72-73 0.12969138954713344 2401 74-75 -0.09180585029064758 2401 76-77 -0.00632158508950198 2401 78-79 0.18805213243565078 2401 80-81 -0.010956010523308635 2401 82-83 0.10257694898856329 2401 84-85 0.25589054907236175 2401 86-87 0.2961065402290828 2401 88-89 0.21896243323239872 2401 90-91 0.01495479486827378 2402 1 0.0771467813990796 2402 2 0.07529201491497872 2402 3 0.09548667256844823 2402 4 0.2083953875279576 2402 5 0.1571484541495849 2402 6 0.1740604650421389 2402 7 0.06253596368829051 2402 8 0.0802611980754051 2402 9 0.08482954280383836 2402 10-11 0.15990168326275978 2402 12-13 0.16965640326737486 2402 14-15 0.08548931037140761 2402 16-17 0.11055983892346433 2402 18-19 0.0810108299910155 2402 20-21 0.08990755331535638 2402 22-23 0.08537641481953528 2402 24-25 0.09487278458891524 2402 26-27 0.04138023994801898 2402 28-29 0.05637197105794911 2402 30-31 0.18997330431839998 2402 32-33 0.06342530841158833 2402 34-35 0.13734363440052277 2402 36-37 0.10040030985909709 2402 38-39 -0.3479140687839646 2402 40-41 0.07596369982694995 2402 42-43 0.19645995030654717 2402 44-45 0.125062811705817 2402 46-47 0.09586095282574547 2402 48-49 0.03090053270884141 2402 50-51 0.18761967135132807 2402 52-53 0.1264924402197849 2402 54-55 0.23301538346868966 2402 56-57 0.23031875315488293 2402 58-59 0.11287218007492328 2402 60-61 0.3232547411965072 2402 62-63 0.22151224116263535 2402 64-65 0.14638036075690763 2402 66-67 0.1691169778078887 2402 68-69 0.19021598294106923 2402 70-71 0.19397489526313905 2402 72-73 0.2042898508410289 2402 74-75 0.0860800471675347 2402 76-77 0.20147559966905249 2402 78-79 0.09131744924551555 2402 80-81 0.0664610201596858 2402 82-83 0.17555861307474885 2402 84-85 0.2576442008260216 2402 86-87 0.48236109452091824 2402 88-89 0.11657593084590445 2402 90-91 0.25384368375716093 2403 1 0.042404667054974254 2403 2 0.027416912313505293 2403 3 0.02690701194898537 2403 4 -0.027998401104632364 2403 5 0.06712718004870766 2403 6 0.11268384645158847 2403 7 0.05305475022697692 2403 8 0.03955563498178094 2403 9 0.023988964550319736 2403 10-11 0.1279737806880874 2403 12-13 0.1440772284344689 2403 14-15 0.029808120361863644 2403 16-17 0.056118242193306855 2403 18-19 0.04516313245177628 2403 20-21 0.024661393541826726 2403 22-23 0.06802210890802485 2403 24-25 0.027985001283212796 2403 26-27 0.11391319805311895 2403 28-29 0.06888516267562039 2403 30-31 0.10978502015001368 2403 32-33 -0.020973954779059056 2403 34-35 0.07061036551514377 2403 36-37 0.10398732193694826 2403 38-39 0.01742809661825362 2403 40-41 0.08959679752179284 2403 42-43 0.046298786440985396 2403 44-45 0.08737022238281611 2403 46-47 0.006818504349823229 2403 48-49 0.025405036868505704 2403 50-51 0.05591152897428486 2403 52-53 0.13107985001917655 2403 54-55 -0.11346478080191957 2403 56-57 -0.041890295058315985 2403 58-59 -0.11768512273779663 2403 60-61 -0.1121951502676879 2403 62-63 -0.0999543969076413 2403 64-65 0.10972506550703542 2403 66-67 0.08042424304314011 2403 68-69 0.051195477382975696 2403 70-71 0.06804798084726826 2403 72-73 -0.11215458991804184 2403 74-75 -0.1850324970628776 2403 76-77 0.048173031260795085 2403 78-79 -0.04386614258203991 2403 80-81 -0.1357992768533549 2403 82-83 -0.03809457545911954 2403 84-85 -0.02711334876158844 2403 86-87 -0.010199054137480346 2403 88-89 -0.29898153927822335 2403 90-91 -0.27932287867768935 2404 1 0.014572524938039066 2404 2 -0.0968301228428956 2404 3 0.02170003303755408 2404 4 0.10908431697435361 2404 5 0.12608481845581565 2404 6 -0.04661131576709465 2404 7 0.03950100811580626 2404 8 -0.02367196608565081 2404 9 0.15183589376750461 2404 10-11 0.008875535853910321 2404 12-13 0.10618026002997283 2404 14-15 0.06948553961994008 2404 16-17 0.049797881616015616 2404 18-19 0.05235872139678577 2404 20-21 0.01776574577834822 2404 22-23 0.04737415797120548 2404 24-25 -0.04356896698360657 2404 26-27 0.04780976093713463 2404 28-29 -0.046423025388698136 2404 30-31 -0.05300205730409857 2404 32-33 -0.07339525592622209 2404 34-35 -0.022297889116572378 2404 36-37 0.046346534614663426 2404 38-39 0.011935707164219878 2404 40-41 -0.01872081376629353 2404 42-43 -0.07218067591853128 2404 44-45 0.02399336804744223 2404 46-47 -0.04111484710263369 2404 48-49 -0.024583985737109515 2404 50-51 -0.03307485204218352 2404 52-53 0.06355707147533707 2404 54-55 -0.055015127515495976 2404 56-57 -0.013118610881747372 2404 58-59 -0.008096003979467525 2404 60-61 -0.20610388557382464 2404 62-63 -0.014498699308951757 2404 64-65 0.1288376076167168 2404 66-67 -0.020008305576773466 2404 68-69 0.09907105805440608 2404 70-71 0.10999359699121669 2404 72-73 -0.06393661634742642 2404 74-75 -0.11097848349721318 2404 76-77 0.21578838662737354 2404 78-79 0.10601061440085147 2404 80-81 0.14679702002668904 2404 82-83 0.09734151342991026 2404 84-85 0.07384731197868177 2404 86-87 -0.00844229938621055 2404 88-89 -0.5075006251092162 2404 90-91 0.35982658974006654 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 13.0 31 274.0 32 2100.0 33 3690.0 34 4973.0 35 6102.0 36 8514.0 37 12721.0 38 31158.0 39 210459.0 40 213448.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.983779577344908 15.028006776748295 28.161604370840525 29.826609275066268 2 26.420616935107898 17.062605656927648 29.967851916114984 26.548925491849467 3 28.52070718124559 19.117968920989277 21.878521112489157 30.482802785275975 4 27.881739257313782 22.21371075606138 21.12262185582387 28.78192813080097 5 27.469541110381556 21.903650203059264 21.7234908359881 28.90331785057108 6 28.109116996182 23.42274425881342 23.204485947974675 25.263652797029906 7 26.639267851787 23.910532331412174 23.27865729594773 26.17154252085309 8 25.512106547344015 24.169726741405444 23.66228123505427 26.655885476196268 9 25.505621620745277 23.7244959996109 23.88641651062312 26.883465869020696 10-11 25.51008000778191 23.22343409288036 23.787014745101853 27.479471154235874 12-13 26.285839352155833 22.849132235759505 23.255656071917834 27.60937234016683 14-15 26.414727268305732 22.45770611933886 23.164259137666885 27.96330747468852 16-17 26.41361267154657 22.64799818422055 22.97619626630351 27.962192877929365 18-19 26.705333041511636 22.806372250999086 22.692582054586868 27.79571265290241 20-21 26.689526032927212 22.625503595081184 22.787728087027716 27.897242284963887 22-23 26.365482356946572 22.668263579841604 23.0318247772833 27.93442928592852 24-25 26.63227629029774 22.725715976427292 22.8066762319334 27.83533150134157 26-27 26.70290119403711 22.684577223316555 22.837986268167928 27.77453531447841 28-29 26.54939082220763 22.780331217626028 23.179964819273202 27.490313140893136 30-31 26.929550177778722 22.70288509181772 22.968463908740596 27.399100821662962 32-33 26.864504085549317 22.601326659943936 23.113652179901916 27.420517074604835 34-35 26.69556053830524 22.644388727393576 23.281091586772064 27.37895914752912 36-37 26.918746187769553 22.73876033013241 23.17738949254827 27.165103989549767 38-39 26.99489018856128 22.555261726255175 23.24157626502043 27.208271820163116 40-41 26.72568387222192 22.739491873823116 23.35914952648585 27.17567472746911 42-43 27.076522400445786 22.715293239807817 23.149359423540975 27.058824936205422 44-45 27.061680306123087 22.69043739895254 23.33239698621073 26.915485308713645 46-47 26.814838064225043 22.717826993873054 23.48902446201228 26.978310479889622 48-49 27.122730282407954 22.83193718284397 23.24446324905145 26.80086928569662 50-51 27.175941297322222 22.6018648336337 23.37609991402221 26.84609395502187 52-53 26.958169470583083 22.752525368950526 23.546289594081422 26.74301556638497 54-55 27.344394401602536 22.76918393799343 23.24951884680344 26.6369028136006 56-57 27.175472860402927 22.721799879597352 23.380864379016337 26.721862880983384 58-59 27.145935041052503 22.72868152984689 23.512163601425332 26.613219827675273 60-61 27.521595734336003 22.759189329357234 23.194750575346017 26.52446436096075 62-63 27.46127430935704 22.699872050230177 23.335443845000505 26.503409795412274 64-65 27.212569990683917 22.81120043026863 23.49598063791166 26.48024894113579 66-67 27.767539853101912 22.729864876995297 22.999952477255086 26.502642792647702 68-69 27.817549361785538 22.55999625526238 22.949390456185107 26.673063926766975 70-71 27.3974036166247 22.708871521532977 23.35437036068147 26.53935450116085 72-73 27.970299768727706 22.76206249147022 22.98448225621429 26.283155483587784 74-75 27.900078787597906 22.582126591024956 22.97143001596818 26.546364605408957 76-77 28.053545091374378 22.524198825954034 23.011593948095836 26.41066213457575 78-79 28.360915239602818 22.679522233414882 22.600086343358758 26.359476183623542 80-81 28.368695748865463 22.3773550812768 22.487855390538673 26.766093779319068 82-83 28.632430512849382 22.335495829471732 22.397858868976986 26.6342147887019 84-85 29.506623152236518 22.055718044560884 21.623208699874652 26.814450103327953 86-87 29.786559259141796 21.03485458754876 21.553391900034885 27.625194253274554 88-89 30.65375801426475 20.985143033704972 20.387774544892757 27.97332440713752 90-91 28.501628664495115 18.967677273866197 20.590077674768228 31.940616386870456 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 3.0 10 8.0 11 11.0 12 10.0 13 11.5 14 16.0 15 24.5 16 40.5 17 61.0 18 85.0 19 127.0 20 164.0 21 208.5 22 293.0 23 405.0 24 524.0 25 670.5 26 911.5 27 1181.5 28 1513.5 29 1880.0 30 2248.0 31 2716.0 32 3279.5 33 3876.0 34 4683.5 35 5714.5 36 6847.0 37 7990.0 38 9163.0 39 10593.5 40 12181.0 41 13940.0 42 15871.0 43 17652.0 44 19417.5 45 21346.5 46 23167.0 47 24962.5 48 26578.0 49 27989.5 50 29330.0 51 30479.0 52 31313.5 53 32005.0 54 32767.5 55 33235.5 56 33181.5 57 33064.0 58 32932.5 59 32500.0 60 31800.0 61 30814.5 62 29687.5 63 28010.5 64 25898.0 65 23784.0 66 21131.5 67 18456.0 68 16051.5 69 13715.5 70 11633.0 71 9635.5 72 7749.0 73 6022.0 74 4612.0 75 3573.0 76 2697.5 77 1948.0 78 1456.0 79 1071.5 80 729.5 81 493.0 82 334.5 83 221.5 84 146.5 85 100.5 86 70.0 87 47.5 88 25.5 89 11.5 90 7.0 91 5.5 92 3.5 93 3.0 94 3.0 95 2.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.022291935183158647 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.06270686873695692 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.640145524821328E-4 68-69 0.0 70-71 0.0 72-73 7.37705220370992E-4 74-75 8.426410054392477E-4 76-77 4.883694808144049E-4 78-79 0.0011512315299291418 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.012526619065514218 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 4579.0 32-33 10491.0 34-35 11954.0 36-37 13206.0 38-39 14607.0 40-41 16162.0 42-43 17413.0 44-45 18164.0 46-47 18182.0 48-49 18993.0 50-51 19479.0 52-53 19684.0 54-55 19952.0 56-57 19592.0 58-59 19319.0 60-61 19434.0 62-63 19261.0 64-65 18866.0 66-67 18695.0 68-69 18121.0 70-71 17484.0 72-73 16967.0 74-75 16496.0 76-77 15629.0 78-79 15134.0 80-81 14402.0 82-83 13601.0 84-85 12925.0 86-87 12285.0 88-89 11612.0 90-91 10763.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.7799030066464 #Duplication Level Percentage of deduplicated Percentage of total 1 99.7935546847093 99.57391207128758 2 0.19452910905407803 0.38820191266770493 3 0.01029451226477339 0.030815563058394616 4 0.0010224206917573146 0.004080681498221326 5 5.992732800900681E-4 0.0029897714880930924 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0020265395621053314 0.0 0.0 0.0 0.0 2 0.0020265395621053314 0.0 0.0 0.0 0.0 3 0.0020265395621053314 0.0 0.0 0.0 0.0 4 0.0020265395621053314 0.0 0.0 0.0 0.0 5 0.0024318474745263978 0.0 0.0 0.0 0.0 6 0.002634501430736931 0.0 0.0 0.0 0.0 7 0.002837155386947464 0.0 0.0 0.0 0.0 8 0.011145967591579323 0.0 0.0 0.0 0.0 9 0.011145967591579323 0.0 0.0 0.0 0.0 10-11 0.011145967591579323 0.0 0.0 0.0 0.0 12-13 0.011145967591579323 0.0 0.0 0.0 0.0 14-15 0.011247294569684589 0.0 0.0 0.0 0.0 16-17 0.011348621547789857 0.0 0.0 0.0 0.0 18-19 0.011348621547789857 0.0 0.0 0.0 0.0 20-21 0.011348621547789857 0.0 0.0 0.0 0.0 22-23 0.011753929460210923 0.0 1.0132697810526657E-4 0.0 0.0 24-25 0.011956583416421456 0.0 2.0265395621053313E-4 0.0 1.0132697810526657E-4 26-27 0.01215923737263199 0.0 2.0265395621053313E-4 0.0 2.0265395621053313E-4 28-29 0.01215923737263199 0.0 2.0265395621053313E-4 0.0 2.0265395621053313E-4 30-31 0.012665872263158321 0.0 2.0265395621053313E-4 0.0 2.0265395621053313E-4 32-33 0.013476488088000452 0.0 2.0265395621053313E-4 0.0 2.0265395621053313E-4 34-35 0.013881796000421519 0.0 2.0265395621053313E-4 0.0 2.0265395621053313E-4 36-37 0.014591084847158386 0.0 2.0265395621053313E-4 0.0 2.0265395621053313E-4 38-39 0.014895065781474186 0.0 2.0265395621053313E-4 0.0 2.0265395621053313E-4 40-41 0.014996392759579452 0.0 2.0265395621053313E-4 0.0 2.0265395621053313E-4 42-43 0.015199046715789985 0.0 2.0265395621053313E-4 0.0 2.0265395621053313E-4 44-45 0.015199046715789985 0.0 2.0265395621053313E-4 0.0 2.0265395621053313E-4 46-47 0.015300373693895253 0.0 2.0265395621053313E-4 0.0 4.0530791242106626E-4 48-49 0.015807008584421585 0.0 3.0398093431579967E-4 0.0 4.0530791242106626E-4 50-51 0.01600966254063212 0.0 4.0530791242106626E-4 0.0 4.0530791242106626E-4 52-53 0.01600966254063212 0.0 4.0530791242106626E-4 0.0 4.0530791242106626E-4 54-55 0.01600966254063212 0.0 4.0530791242106626E-4 0.0 4.0530791242106626E-4 56-57 0.016110989518737386 0.0 4.0530791242106626E-4 0.0 4.0530791242106626E-4 58-59 0.016617624409263718 0.0 4.0530791242106626E-4 0.0 4.0530791242106626E-4 60-61 0.01682027836547425 0.0 4.0530791242106626E-4 0.0 4.0530791242106626E-4 62-63 0.01702293232168478 0.0 4.0530791242106626E-4 0.0 4.0530791242106626E-4 64-65 0.017934875124632182 0.0 4.0530791242106626E-4 0.0 4.0530791242106626E-4 66-67 0.018137529080842714 0.0 4.0530791242106626E-4 0.0 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