##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-28A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 492956 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.56279262246529 40.0 40.0 40.0 40.0 40.0 2 39.35071689968273 40.0 40.0 40.0 40.0 40.0 3 39.304266506544195 40.0 40.0 40.0 40.0 40.0 4 39.201616371440856 40.0 40.0 40.0 40.0 40.0 5 39.2188187181006 40.0 40.0 40.0 40.0 40.0 6 39.34942672368325 40.0 40.0 40.0 40.0 40.0 7 39.37550613036458 40.0 40.0 40.0 40.0 40.0 8 39.37041845519681 40.0 40.0 40.0 40.0 40.0 9 39.39812883908503 40.0 40.0 40.0 40.0 40.0 10-11 39.40104187797694 40.0 40.0 40.0 40.0 40.0 12-13 39.3325895211743 40.0 40.0 40.0 40.0 40.0 14-15 39.36288431421871 40.0 40.0 40.0 40.0 40.0 16-17 39.46914126210047 40.0 40.0 40.0 40.0 40.0 18-19 39.50617499330569 40.0 40.0 40.0 40.0 40.0 20-21 39.48573909233278 40.0 40.0 40.0 40.0 40.0 22-23 39.52541809005266 40.0 40.0 40.0 40.0 40.0 24-25 39.502706123873125 40.0 40.0 40.0 40.0 40.0 26-27 39.474370937771326 40.0 40.0 40.0 40.0 40.0 28-29 39.50086011733299 40.0 40.0 40.0 40.0 40.0 30-31 39.476734762959794 40.0 40.0 40.0 40.0 40.0 32-33 39.47794905017223 40.0 40.0 40.0 40.0 40.0 34-35 39.4811877623747 40.0 40.0 40.0 40.0 40.0 36-37 39.46225576712463 40.0 40.0 40.0 40.0 40.0 38-39 39.407500560705685 40.0 40.0 40.0 40.0 40.0 40-41 39.44848132331244 40.0 40.0 40.0 40.0 40.0 42-43 39.4281813017305 40.0 40.0 40.0 40.0 40.0 44-45 39.33760855831833 40.0 40.0 40.0 40.0 40.0 46-47 39.326067482706755 40.0 40.0 40.0 40.0 40.0 48-49 39.40198894199459 40.0 40.0 40.0 40.0 40.0 50-51 39.353805402128614 40.0 40.0 40.0 40.0 40.0 52-53 39.32251804829939 40.0 40.0 40.0 40.0 40.0 54-55 39.22657258433598 40.0 40.0 40.0 40.0 40.0 56-57 39.246048458020326 40.0 40.0 40.0 40.0 40.0 58-59 39.20000190228335 40.0 40.0 40.0 40.0 40.0 60-61 39.12708705742929 40.0 40.0 40.0 40.0 40.0 62-63 39.374830872239826 40.0 40.0 40.0 40.0 40.0 64-65 39.38311713435458 40.0 40.0 40.0 40.0 40.0 66-67 39.376686477655895 40.0 40.0 40.0 40.0 40.0 68-69 39.381346005580596 40.0 40.0 40.0 40.0 40.0 70-71 39.38825558942481 40.0 40.0 40.0 40.0 40.0 72-73 39.37561700629422 40.0 40.0 40.0 40.0 40.0 74-75 39.381471599845696 40.0 40.0 40.0 40.0 40.0 76-77 39.37501358633314 40.0 40.0 40.0 40.0 40.0 78-79 39.38109465933097 40.0 40.0 40.0 40.0 40.0 80-81 39.37960307159176 40.0 40.0 40.0 40.0 40.0 82-83 39.360405915304874 40.0 40.0 40.0 40.0 40.0 84-85 39.35391191411428 40.0 40.0 40.0 40.0 40.0 86-87 39.341631382411684 40.0 40.0 40.0 40.0 40.0 88-89 39.37070834063736 40.0 40.0 40.0 40.0 40.0 90-91 39.45817793583963 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.12543793810184667 1101 2 -0.1327062029803301 1101 3 -0.11492806217925988 1101 4 -0.1981060003709132 1101 5 -0.1710624282001021 1101 6 -0.13075240081059292 1101 7 -0.1829837097123601 1101 8 -0.13115427123467782 1101 9 -0.10711691607948381 1101 10-11 -0.11095970363034269 1101 12-13 -0.051689902935606824 1101 14-15 -0.03918315203845424 1101 16-17 -0.0035564742407458994 1101 18-19 0.025269041952689975 1101 20-21 -0.03372091675422695 1101 22-23 -0.03344114706890622 1101 24-25 -0.11300186926844447 1101 26-27 -0.061999004590518325 1101 28-29 -0.0976560929014596 1101 30-31 -0.0489713753486285 1101 32-33 -0.0959023398232759 1101 34-35 -0.1096364980016773 1101 36-37 -0.09941018964622828 1101 38-39 -0.12556021654526717 1101 40-41 -0.20291484959641082 1101 42-43 4.866277322150836E-4 1101 44-45 -0.20388000314103039 1101 46-47 -0.010908484965725052 1101 48-49 -0.15966339432610965 1101 50-51 -0.12635115967178479 1101 52-53 -0.09293747078280035 1101 54-55 -0.08868027060844952 1101 56-57 0.055276589201120885 1101 58-59 -0.14440921968798648 1101 60-61 -0.04237418719859676 1101 62-63 0.06767855493087893 1101 64-65 -0.15677124115532592 1101 66-67 -0.11658095287504722 1101 68-69 -0.09743843850390732 1101 70-71 -0.20659516917655907 1101 72-73 -0.19833756672321812 1101 74-75 -0.377708890108444 1101 76-77 -0.14495238449659098 1101 78-79 -0.22202757177231547 1101 80-81 -0.19443107838129947 1101 82-83 -0.10155467900863613 1101 84-85 -0.38607367847924934 1101 86-87 0.1977337440901792 1101 88-89 0.42572372252211466 1101 90-91 0.5711716705339569 1102 1 0.009519180251494674 1102 2 0.009117696390738672 1102 3 -0.015271115352497588 1102 4 0.0442153261013587 1102 5 -0.031679923911937635 1102 6 -0.23646995369796286 1102 7 -0.21016495613888964 1102 8 -0.046728885299856415 1102 9 -0.2191923877775963 1102 10-11 -0.12184020677499063 1102 12-13 0.026514785457763423 1102 14-15 -0.0019104247657253381 1102 16-17 -0.05435693164497479 1102 18-19 0.0043713861493728245 1102 20-21 -0.10111371263758429 1102 22-23 0.04097852131302204 1102 24-25 -0.04277888470583946 1102 26-27 -0.13379431619714666 1102 28-29 -0.19054345710957676 1102 30-31 -0.01974324498841895 1102 32-33 -0.054033192441941935 1102 34-35 -0.06807918426721926 1102 36-37 -0.05299232389720743 1102 38-39 0.011029213204935218 1102 40-41 -0.03771778243607571 1102 42-43 -0.030264215049967902 1102 44-45 -0.23015590473640657 1102 46-47 -0.10320403201286865 1102 48-49 -0.001151008662162667 1102 50-51 0.033953513334729735 1102 52-53 -0.05243699956292858 1102 54-55 -0.1489637143657987 1102 56-57 -0.04287314894572347 1102 58-59 0.02129887384090523 1102 60-61 -0.023315443175853545 1102 62-63 0.17665717029259298 1102 64-65 0.0632312525647265 1102 66-67 -0.1395514891390519 1102 68-69 -0.2505580000631795 1102 70-71 -0.021532529309205017 1102 72-73 0.04684813052035253 1102 74-75 -0.08334073176222034 1102 76-77 -0.24700786562183907 1102 78-79 -0.02757123733046285 1102 80-81 -0.11093829570653924 1102 82-83 0.1145606777360797 1102 84-85 -0.23619002285860802 1102 86-87 0.27688502247259095 1102 88-89 0.5086066054049994 1102 90-91 0.5711716705339569 1103 1 0.09474912871734631 1103 2 0.1813451170295366 1103 3 0.1553027432847074 1103 4 -0.03565618766203471 1103 5 0.11393185213096757 1103 6 -0.02392020019600949 1103 7 -0.031461829174936895 1103 8 -0.01275712434011922 1103 9 0.04810144069328004 1103 10-11 -0.056025087296760034 1103 12-13 -0.0883653173312311 1103 14-15 -0.06213248250073633 1103 16-17 0.03691298721317082 1103 18-19 -0.07811677775437431 1103 20-21 0.0349453910312576 1103 22-23 -0.08217721238528242 1103 24-25 0.0371697207995183 1103 26-27 0.09921603253125966 1103 28-29 0.019390763523638554 1103 30-31 0.05828923968400801 1103 32-33 0.09662620742177808 1103 34-35 0.009880576722324008 1103 36-37 0.009202307345837824 1103 38-39 0.07485777085252465 1103 40-41 0.04192794946089151 1103 42-43 0.03327784600162431 1103 44-45 0.057367677787006244 1103 46-47 -0.01947797584040245 1103 48-49 0.0999117288353446 1103 50-51 -0.05375725201445647 1103 52-53 -0.046310961278706486 1103 54-55 -0.049512214711505464 1103 56-57 0.10437592612290558 1103 58-59 0.09513401050617176 1103 60-61 0.013794953122221898 1103 62-63 -0.23368433811833 1103 64-65 -0.04886491101018464 1103 66-67 0.037329775289130396 1103 68-69 -0.23054018489118278 1103 70-71 -0.23534488548743582 1103 72-73 -0.08363829516166987 1103 74-75 -0.06331214280468345 1103 76-77 -0.17962868395694187 1103 78-79 0.08669301712711786 1103 80-81 0.039913183913775185 1103 82-83 0.001876283537036727 1103 84-85 0.0986573957326442 1103 86-87 0.09338424774374232 1103 88-89 0.20429287991480294 1103 90-91 -0.021420922058638325 1104 1 0.02890960115135499 1104 2 -0.1026384719692075 1104 3 0.0062092910335707074 1104 4 -0.06539280161997851 1104 5 0.007681469411721764 1104 6 0.10547489390726383 1104 7 0.1176144342820109 1104 8 -0.04638055438548605 1104 9 0.052970166649110695 1104 10-11 -0.032899713306193235 1104 12-13 0.05716790592220633 1104 14-15 0.022704959849662032 1104 16-17 0.027791108993632463 1104 18-19 0.043268338253874106 1104 20-21 -0.029821985496802483 1104 22-23 0.04510043713305123 1104 24-25 -0.04382387744894345 1104 26-27 0.028934275965404765 1104 28-29 -0.03820673722568557 1104 30-31 0.023333678088505394 1104 32-33 0.019779762290319525 1104 34-35 0.013709033145822502 1104 36-37 -0.09492104678076885 1104 38-39 -0.05187159481107528 1104 40-41 -0.1316160145737939 1104 42-43 -0.07550249492274475 1104 44-45 -0.07504119772593754 1104 46-47 0.03933320425979048 1104 48-49 -0.019340167805864894 1104 50-51 0.051996249375442005 1104 52-53 -0.06273734987031787 1104 54-55 -0.07680031601615411 1104 56-57 -0.06800951074431083 1104 58-59 -0.1132848738326544 1104 60-61 0.012398803612185816 1104 62-63 0.10656188613567252 1104 64-65 0.07582554413654208 1104 66-67 0.03790850604782037 1104 68-69 0.15370923802048253 1104 70-71 0.39228476666271916 1104 72-73 0.09232488563156949 1104 74-75 -0.02342415909193818 1104 76-77 0.22706210407655192 1104 78-79 0.05928214779075347 1104 80-81 0.030512615750218686 1104 82-83 0.007687106202858729 1104 84-85 0.05779558485248515 1104 86-87 0.1917252365609201 1104 88-89 0.2516960362993075 1104 90-91 0.5711716705339569 1201 1 0.10123401013872524 1201 2 -0.0116822378387198 1201 3 0.12190431386880363 1201 4 -0.13284555275752297 1201 5 -0.15263633722499037 1201 6 0.006311044095987484 1201 7 0.030107991614634955 1201 8 -0.07011908274850498 1201 9 -0.17109658201992772 1201 10-11 -0.04498294255554214 1201 12-13 0.023315048539934935 1201 14-15 -0.015505196007552513 1201 16-17 0.034685421844287134 1201 18-19 0.010992287479858476 1201 20-21 -0.008425478713682821 1201 22-23 -0.04817673827509594 1201 24-25 -0.05728786649561357 1201 26-27 0.060536811082556596 1201 28-29 0.1284414841765127 1201 30-31 0.027347367636778586 1201 32-33 0.07920843858331494 1201 34-35 0.0014380222209595672 1201 36-37 -0.0859924409000925 1201 38-39 0.006902051295803346 1201 40-41 0.01974327129271103 1201 42-43 -0.026491936560290696 1201 44-45 0.07423750692593956 1201 46-47 0.014209385830980636 1201 48-49 -0.06498845113475227 1201 50-51 -0.12422561228395068 1201 52-53 -0.14804643093488323 1201 54-55 -0.10453232017299996 1201 56-57 0.047774084558660945 1201 58-59 -0.03853114965999538 1201 60-61 -0.2149374200266294 1201 62-63 0.0562960374038326 1201 64-65 -0.11802876583844579 1201 66-67 -0.13957199242300788 1201 68-69 -0.1216994777532534 1201 70-71 0.07982389374207344 1201 72-73 0.004858188700971766 1201 74-75 0.07649890089155775 1201 76-77 0.0113884414470391 1201 78-79 -0.18963869980346715 1201 80-81 -0.08768421868361997 1201 82-83 -0.24747795595777689 1201 84-85 -0.21286184116539886 1201 86-87 0.18502717272000524 1201 88-89 0.5259979097528245 1201 90-91 0.5711716705339569 1202 1 0.0031619291841522568 1202 2 -0.1435334535886028 1202 3 -0.14379335746790645 1202 4 0.07543967536454232 1202 5 -0.028859375778068852 1202 6 -0.01743676267744121 1202 7 0.1059567901770464 1202 8 -0.1584269985074016 1202 9 -0.10200742702838284 1202 10-11 -0.0015923070366667957 1202 12-13 -0.08511171226982839 1202 14-15 -0.09913670213798298 1202 16-17 -0.04815943818677226 1202 18-19 -0.1155294539552969 1202 20-21 -0.03149795723199844 1202 22-23 -0.15748450030934436 1202 24-25 0.004118236902755257 1202 26-27 0.058228821111768525 1202 28-29 -0.12302658615516293 1202 30-31 -0.011546026974372126 1202 32-33 -0.06729866962741227 1202 34-35 -0.1471460874301158 1202 36-37 -0.32788887522416843 1202 38-39 -0.17923649067045488 1202 40-41 -0.24454816600234608 1202 42-43 -0.21239939643810857 1202 44-45 -0.13481396243562926 1202 46-47 -0.33028177154039895 1202 48-49 -0.11825939809580888 1202 50-51 -0.27947576395363427 1202 52-53 -0.11186248378102448 1202 54-55 -0.22182961796487177 1202 56-57 -0.1462569231077211 1202 58-59 -0.07028937124734114 1202 60-61 -0.1960279154465283 1202 62-63 -0.12701867902204356 1202 64-65 -0.06336773136402485 1202 66-67 -0.2408229534760551 1202 68-69 -0.3557642049447125 1202 70-71 -0.3593463120043481 1202 72-73 -0.5773252981439825 1202 74-75 -0.2465636565454119 1202 76-77 -0.3313005222829162 1202 78-79 -0.11340375885840359 1202 80-81 -0.7373053241920999 1202 82-83 -0.37496571951927393 1202 84-85 -0.2184813052215091 1202 86-87 -0.7728681619195257 1202 88-89 -1.213615616817222 1202 90-91 0.12672722608951403 1203 1 -0.04544311073859575 1203 2 0.021893133197949055 1203 3 -0.005694905577676934 1203 4 0.0033897537564229197 1203 5 -0.02275000094030588 1203 6 0.025362086882836365 1203 7 -0.13139096864593824 1203 8 -0.03552514928976791 1203 9 -0.0017115106740774877 1203 10-11 -0.021604559012033064 1203 12-13 -0.020192855649085573 1203 14-15 -0.18694598664339424 1203 16-17 -0.01333060243592854 1203 18-19 0.0023262426940959813 1203 20-21 -0.02197256553218807 1203 22-23 -0.041389765543847545 1203 24-25 0.006757603122608202 1203 26-27 -0.021184745355199652 1203 28-29 -0.04765824816677622 1203 30-31 0.022798959579603206 1203 32-33 -0.010852922082101202 1203 34-35 -0.08545354953092499 1203 36-37 -0.10934265904369767 1203 38-39 -0.005447450557255706 1203 40-41 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0.02691795271461217 2401 32-33 0.015833169977874206 2401 34-35 0.1100638876196598 2401 36-37 0.06055818432214011 2401 38-39 0.12337546016432555 2401 40-41 0.08217893502791185 2401 42-43 0.11172589271471622 2401 44-45 0.1178292448078011 2401 46-47 -0.0010364647342271383 2401 48-49 -0.03573310416479103 2401 50-51 -0.0065998921151688705 2401 52-53 -0.02262292411134581 2401 54-55 -0.0387461070856574 2401 56-57 -0.037615072710401876 2401 58-59 -0.1327514789078137 2401 60-61 -0.012817809730570673 2401 62-63 0.09896988963266296 2401 64-65 -0.06529451352707838 2401 66-67 0.03672832150416383 2401 68-69 0.26688382224730134 2401 70-71 -0.027152977171645887 2401 72-73 0.08896994522677204 2401 74-75 0.07953120264779301 2401 76-77 0.18863921837306918 2401 78-79 -0.016506916055618603 2401 80-81 0.31583815316347597 2401 82-83 0.2622282298803569 2401 84-85 0.28418615268997627 2401 86-87 0.07237993539744281 2401 88-89 0.01916723121986763 2401 90-91 0.37117167053395406 2402 1 -0.1096227402832568 2402 2 0.032630525938742494 2402 3 0.01730942302936711 2402 4 -0.04543326280852966 2402 5 0.022799793336140795 2402 6 0.058736717880556455 2402 7 -0.11040563696450789 2402 8 0.014521501775561774 2402 9 0.11297363287354756 2402 10-11 -0.03379501772202076 2402 12-13 -0.06768516969487592 2402 14-15 0.008774351691549498 2402 16-17 0.04988541493968057 2402 18-19 -0.07967256426226754 2402 20-21 0.04652037562629374 2402 22-23 -0.0306217819405461 2402 24-25 0.007124693472718491 2402 26-27 -0.036709608078709266 2402 28-29 -0.01874988309541692 2402 30-31 -0.038887514670541634 2402 32-33 0.04865520005474622 2402 34-35 -0.002229602770299266 2402 36-37 0.07429863017037519 2402 38-39 -0.2327977719628862 2402 40-41 -0.06581090255703259 2402 42-43 0.029155025389513867 2402 44-45 0.007664514630228325 2402 46-47 0.07497160316378171 2402 48-49 0.04218804898336259 2402 50-51 -0.051251315309336576 2402 52-53 0.028721071339681714 2402 54-55 0.026762265191614176 2402 56-57 -0.08255384188758796 2402 58-59 0.04188565007837042 2402 60-61 0.02010799878977565 2402 62-63 0.056390658540955485 2402 64-65 0.20845996827796398 2402 66-67 0.07161197919599971 2402 68-69 0.29029014105591955 2402 70-71 0.1064326455237321 2402 72-73 0.11547871247918096 2402 74-75 0.027639703054646247 2402 76-77 0.12628744341785136 2402 78-79 0.1664618469223882 2402 80-81 0.10709266577332954 2402 82-83 0.29494364640903115 2402 84-85 0.1196967133559923 2402 86-87 0.3239346420622482 2402 88-89 0.43611026930338426 2402 90-91 -0.062368702136851084 2403 1 -0.011214654398408186 2403 2 0.022343172834837333 2403 3 -0.07721781267471073 2403 4 0.1261567501075973 2403 5 0.10571484706795076 2403 6 0.10406813546326532 2403 7 0.08184512644123743 2403 8 0.031176131732955525 2403 9 -0.07117504911469297 2403 10-11 0.04785341356735273 2403 12-13 0.07668891035097403 2403 14-15 0.045579793233159194 2403 16-17 -0.02912095075546972 2403 18-19 0.07934106458256451 2403 20-21 -0.022716800691412686 2403 22-23 0.09313710047076285 2403 24-25 0.051147731829175314 2403 26-27 0.00469730450377881 2403 28-29 -0.04271258289926294 2403 30-31 -0.044601234589443095 2403 32-33 -0.022239689324862866 2403 34-35 -0.004913100008621996 2403 36-37 0.15816045138645762 2403 38-39 0.06607675202270258 2403 40-41 0.09520487143221601 2403 42-43 0.15532586596432907 2403 44-45 0.11737702121643423 2403 46-47 0.06171198097601405 2403 48-49 0.010971100500221098 2403 50-51 0.12098158061999698 2403 52-53 0.08572316810052882 2403 54-55 0.061246989691618126 2403 56-57 0.14482181958928209 2403 58-59 0.0445493999887816 2403 60-61 0.10016141984535665 2403 62-63 -0.1277945624467094 2403 64-65 0.12264747334884873 2403 66-67 0.06011728350421919 2403 68-69 0.09086719533973309 2403 70-71 0.09812870295839815 2403 72-73 0.21374207947047097 2403 74-75 0.2970053468401517 2403 76-77 0.004128014727527329 2403 78-79 0.24300092458892664 2403 80-81 0.1511216925626755 2403 82-83 0.08780669906887084 2403 84-85 0.2503836944843414 2403 86-87 -0.05767868510780971 2403 88-89 0.30712512392351243 2403 90-91 0.3660434654057525 2404 1 -0.09631289417834665 2404 2 -0.00646839697996171 2404 3 6.233659618999354E-4 2404 4 0.05724429998215186 2404 5 -0.03204832248251677 2404 6 -0.23381114953472348 2404 7 -0.04421390230643851 2404 8 -0.13368928453721907 2404 9 -0.04985573441189217 2404 10-11 0.023708067398558796 2404 12-13 -0.013256424027247249 2404 14-15 0.03952524613433184 2404 16-17 -0.18461749862037635 2404 18-19 -0.01840764763785785 2404 20-21 -0.015175161019065797 2404 22-23 -0.04784470780047201 2404 24-25 0.027839453958300453 2404 26-27 -0.05208568029287619 2404 28-29 -0.07603234576694717 2404 30-31 0.0193986863952631 2404 32-33 -0.06358238415456441 2404 34-35 0.04581697793428674 2404 36-37 0.07407270101661112 2404 38-39 -0.021686273741913453 2404 40-41 -0.07499444351020657 2404 42-43 -0.10322688838456173 2404 44-45 0.0024161963590998425 2404 46-47 -0.009137640307031347 2404 48-49 0.021257964834362042 2404 50-51 0.14259636815005194 2404 52-53 0.0804203753210544 2404 54-55 -0.02141735362419439 2404 56-57 0.02705962958024344 2404 58-59 0.1005543873899768 2404 60-61 -0.016422656365918442 2404 62-63 -0.01884278821363239 2404 64-65 -0.199239571407702 2404 66-67 -0.1797547165625346 2404 68-69 0.05520772180972244 2404 70-71 0.0592970692987862 2404 72-73 -0.027801823500119838 2404 74-75 0.24106738508243808 2404 76-77 -0.078512345002693 2404 78-79 -0.04694145031898245 2404 80-81 0.11137371858668388 2404 82-83 -0.052546496657576824 2404 84-85 -0.07069276843517969 2404 86-87 0.19398020759310697 2404 88-89 0.03638665168239186 2404 90-91 -0.40985813976413965 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 13.0 31 319.0 32 2164.0 33 3761.0 34 5040.0 35 6243.0 36 8672.0 37 12564.0 38 31596.0 39 211348.0 40 211236.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.93769829355967 15.477040547229368 26.770340557777978 30.814920601432988 2 26.73603220465539 16.846370483394473 28.43006363016589 27.987533681784242 3 28.173362680163507 18.812264811189664 21.05425444513191 31.960118063514926 4 27.653583686982202 21.816957294362986 20.3817379238715 30.147721094783307 5 27.617880703348778 21.354847085743963 20.89253401926338 30.13473819164388 6 28.172494096836232 23.05297024480887 22.78803787761991 25.986497780734997 7 26.675200220709357 23.56721492384716 22.849098093947536 26.908486761495954 8 25.448315874033383 23.932561932505134 23.244468066115434 27.374654127346048 9 25.690731018589897 23.511834727643034 23.370442798140196 27.42699145562687 10-11 25.518808980923247 23.01949869765253 23.325509781806083 28.136182539618144 12-13 26.292204578096218 22.81674226502974 22.79250075057409 28.098552406299955 14-15 26.3779120246026 22.368527819927134 22.72839766632316 28.525162489147103 16-17 26.39464779818077 22.45535098467206 22.491459684028595 28.658541533118576 18-19 26.636657227014176 22.577471417327306 22.370049253888784 28.415822101769734 20-21 26.647408693676518 22.547042738094273 22.31629192057709 28.489256647652123 22-23 26.322836115190807 22.50829688653754 22.589135744366637 28.579731253905017 24-25 26.67144735027061 22.563778511672442 22.257767427518885 28.507006710538057 26-27 26.617487159097365 22.5329441167163 22.450279538133223 28.399289186053117 28-29 26.516869659766794 22.582948579589253 22.720283351861017 28.179898408782933 30-31 26.946694499047574 22.56836886424817 22.545344263425775 27.939592373278483 32-33 26.863581996371583 22.437159449494214 22.62208307449177 28.077175479642435 34-35 26.703619825897828 22.53530346462263 22.7931309356012 27.967945773878338 36-37 26.9699486928903 22.553302386187802 22.572087846458725 27.904661074463178 38-39 27.253374846903753 22.34195756822597 22.630092553129444 27.774575031740834 40-41 26.870794156274336 22.505201008821377 22.91663276809893 27.70737206680536 42-43 27.168617165684143 22.528990847036923 22.687761790257525 27.614630197021405 44-45 27.203611158347314 22.43725269732818 22.70044999885787 27.65868614546664 46-47 27.051903657904862 22.601401693057717 22.857211341915363 27.489483307122054 48-49 27.356937761476946 22.534265396996307 22.686104287941497 27.42269255358525 50-51 27.479699622601235 22.321669448487885 22.643925895840315 27.55470503307057 52-53 27.378522424448605 22.45657802725331 22.93473946816352 27.230160080134564 54-55 27.57438704270674 22.398993673511544 22.661995556175086 27.364623727606634 56-57 27.553449732976105 22.3951234423606 22.742883858090735 27.308542966572563 58-59 27.480094934081066 22.462647224105066 22.85234815085172 27.204909690962143 60-61 28.122436060289285 22.452511151185504 22.489785888908788 26.935266899616423 62-63 27.925555957331404 22.346546736575664 22.4392062918098 27.288691014283135 64-65 27.75669248757312 22.479745363225334 22.62839871693883 27.135163432262715 66-67 28.030630234181668 22.451939923886204 22.411737062420375 27.105692779511752 68-69 28.066066732437527 22.23904810169095 22.5020316000012 27.19285356587032 70-71 28.12491644608433 22.273468623833587 22.488035079275956 27.11357985080613 72-73 28.57277143587661 22.259697092752184 22.07132006798117 27.096211403390036 74-75 28.31112936485527 22.08717303693816 22.22578740662782 27.37591019157875 76-77 28.3230493777837 22.33714604203351 22.07474487405237 27.265059706130423 78-79 28.908614314563962 22.164486132729998 21.676686900015625 27.25021265269042 80-81 28.884837773711205 21.970651062142096 21.7744348346426 27.3700763295041 82-83 29.429938553159264 21.817931461815178 21.403122256837467 27.34900772818809 84-85 30.394472921774014 21.274793848896813 20.529306886561177 27.801426342768 86-87 30.920477261369317 20.405422288855572 20.324212893553224 28.349887556221887 88-89 31.381316531592184 20.217982266710706 19.3719671756307 29.02873402606641 90-91 29.93775805755839 18.007025328156775 19.529179762124855 32.526036852159976 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.5 8 3.5 9 4.0 10 2.5 11 2.0 12 3.0 13 3.5 14 8.0 15 20.0 16 31.0 17 37.0 18 49.0 19 73.5 20 106.0 21 138.0 22 184.0 23 251.0 24 356.0 25 493.5 26 658.5 27 841.0 28 1065.0 29 1357.0 30 1706.0 31 2126.5 32 2668.5 33 3233.0 34 3853.0 35 4626.0 36 5636.0 37 6733.0 38 7895.0 39 9320.5 40 10865.5 41 12476.0 42 14168.5 43 15991.0 44 17831.5 45 19693.5 46 21772.5 47 23719.5 48 25324.0 49 27008.0 50 28637.0 51 30268.5 52 31672.0 53 32567.0 54 33357.0 55 34057.5 56 34614.0 57 34941.5 58 34937.0 59 34671.0 60 34162.0 61 33114.5 62 31837.0 63 30284.5 64 28109.0 65 25791.0 66 23140.0 67 20475.0 68 18004.5 69 15353.5 70 12754.5 71 10383.5 72 8302.0 73 6488.5 74 4996.5 75 3828.0 76 2890.5 77 2137.0 78 1584.0 79 1140.0 80 795.5 81 534.5 82 337.0 83 222.0 84 155.0 85 107.0 86 63.5 87 34.5 88 19.5 89 11.5 90 8.0 91 7.0 92 6.0 93 4.5 94 3.5 95 2.0 96 0.5 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.021908649047785198 3 2.0285786155356666E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.062321218805764036 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.2599840445126457E-4 64-65 2.4693187149665407E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 5.786434283465843E-4 80-81 6.957973838018369E-4 82-83 8.605926040671606E-4 84-85 0.0 86-87 0.0015616947511439413 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 4954.0 32-33 11367.0 34-35 12695.0 36-37 14073.0 38-39 15485.0 40-41 17191.0 42-43 18561.0 44-45 18591.0 46-47 18619.0 48-49 19181.0 50-51 19815.0 52-53 19729.0 54-55 19776.0 56-57 19259.0 58-59 18755.0 60-61 18963.0 62-63 18807.0 64-65 18581.0 66-67 17552.0 68-69 17185.0 70-71 16693.0 72-73 16302.0 74-75 15695.0 76-77 14982.0 78-79 14753.0 80-81 13979.0 82-83 13241.0 84-85 13006.0 86-87 12529.0 88-89 11690.0 90-91 10947.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.77455540109584 #Duplication Level Percentage of deduplicated Percentage of total 1 99.79134828875078 99.56637408386015 2 0.19678254083224928 0.3926778104447132 3 0.009472909862767026 0.028354661097367076 4 0.0013636156942321376 0.005442165985198727 5 0.0 0.0 6 5.468608335001538E-4 0.0032737677917250327 7 0.0 0.0 8 4.857840264561523E-4 0.0038775108208493414 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.002231436477089233 0.0 0.0 0.0 2.0285786155356663E-4 2 0.002231436477089233 0.0 0.0 0.0 2.0285786155356663E-4 3 0.002231436477089233 0.0 0.0 0.0 2.0285786155356663E-4 4 0.002231436477089233 0.0 0.0 0.0 2.0285786155356663E-4 5 0.002840010061749933 0.0 0.0 0.0 2.0285786155356663E-4 6 0.002840010061749933 0.0 0.0 0.0 2.0285786155356663E-4 7 0.0036514415079642 0.0 0.0 0.0 4.0571572310713327E-4 8 0.0103457509392319 0.0 0.0 0.0 4.0571572310713327E-4 9 0.010548608800785465 0.0 0.0 0.0 4.0571572310713327E-4 10-11 0.010548608800785465 0.0 0.0 0.0 4.0571572310713327E-4 12-13 0.010548608800785465 0.0 2.0285786155356663E-4 0.0 4.0571572310713327E-4 14-15 0.01065003773156225 0.0 2.0285786155356663E-4 0.0 4.0571572310713327E-4 16-17 0.010751466662339032 0.0 2.0285786155356663E-4 0.0 4.0571572310713327E-4 18-19 0.010751466662339032 0.0 2.0285786155356663E-4 0.0 4.0571572310713327E-4 20-21 0.010852895593115815 1.0142893077678332E-4 2.0285786155356663E-4 0.0 4.0571572310713327E-4 22-23 0.010954324523892599 2.0285786155356663E-4 2.0285786155356663E-4 0.0 4.0571572310713327E-4 24-25 0.010954324523892599 2.0285786155356663E-4 2.0285786155356663E-4 0.0 4.0571572310713327E-4 26-27 0.011055753454669384 3.0428679233034995E-4 2.0285786155356663E-4 0.0 4.0571572310713327E-4 28-29 0.011157182385446166 4.0571572310713327E-4 2.0285786155356663E-4 0.0 4.0571572310713327E-4 30-31 0.011157182385446166 4.0571572310713327E-4 2.0285786155356663E-4 0.0 4.0571572310713327E-4 32-33 0.011157182385446166 4.0571572310713327E-4 2.0285786155356663E-4 0.0 4.0571572310713327E-4 34-35 0.011157182385446166 4.0571572310713327E-4 2.0285786155356663E-4 0.0 4.0571572310713327E-4 36-37 0.011360040246999732 4.0571572310713327E-4 2.0285786155356663E-4 0.0 4.0571572310713327E-4 38-39 0.011461469177776514 4.0571572310713327E-4 2.0285786155356663E-4 0.0 4.0571572310713327E-4 40-41 0.011562898108553299 4.0571572310713327E-4 2.0285786155356663E-4 0.0 4.0571572310713327E-4 42-43 0.011562898108553299 4.0571572310713327E-4 2.0285786155356663E-4 0.0 4.0571572310713327E-4 44-45 0.011765755970106866 4.0571572310713327E-4 2.0285786155356663E-4 0.0 4.0571572310713327E-4 46-47 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