##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-27C_S21_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1115621
Sequences flagged as poor quality	0
Sequence length	61
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.503117994372644	40.0	40.0	40.0	40.0	40.0
2	39.15977020869991	40.0	40.0	40.0	40.0	40.0
3	39.111495749900726	40.0	40.0	40.0	40.0	40.0
4	38.996411863885676	40.0	40.0	40.0	40.0	40.0
5	39.04016148853419	40.0	40.0	40.0	40.0	40.0
6	39.182392586729726	40.0	40.0	40.0	40.0	40.0
7	39.19323139309855	40.0	40.0	40.0	40.0	40.0
8	39.20995033259503	40.0	40.0	40.0	40.0	40.0
9	39.22753336482551	40.0	40.0	40.0	40.0	40.0
10	39.2392434348224	40.0	40.0	40.0	40.0	40.0
11	39.226342996411866	40.0	40.0	40.0	40.0	40.0
12	39.233567672175404	40.0	40.0	40.0	40.0	40.0
13	39.04583725118118	40.0	40.0	40.0	40.0	40.0
14	39.235657987793346	40.0	40.0	40.0	40.0	40.0
15	39.13515790756897	40.0	40.0	40.0	40.0	40.0
16	39.320507591735904	40.0	40.0	40.0	40.0	40.0
17	39.26679400979365	40.0	40.0	40.0	40.0	40.0
18	39.365530050079734	40.0	40.0	40.0	40.0	40.0
19	39.311981398700816	40.0	40.0	40.0	40.0	40.0
20	39.32461292858417	40.0	40.0	40.0	40.0	40.0
21	39.30324007884398	40.0	40.0	40.0	40.0	40.0
22	39.36641924094294	40.0	40.0	40.0	40.0	40.0
23	39.356089568052234	40.0	40.0	40.0	40.0	40.0
24	39.368362553232686	40.0	40.0	40.0	40.0	40.0
25	39.32284530319885	40.0	40.0	40.0	40.0	40.0
26	39.28561760669618	40.0	40.0	40.0	40.0	40.0
27	39.30901981945482	40.0	40.0	40.0	40.0	40.0
28	39.31767508858295	40.0	40.0	40.0	40.0	40.0
29	39.31625525155945	40.0	40.0	40.0	40.0	40.0
30	39.315810656127844	40.0	40.0	40.0	40.0	40.0
31	39.263975848428814	40.0	40.0	40.0	40.0	40.0
32	39.29561024756615	40.0	40.0	40.0	40.0	40.0
33	39.294613493292076	40.0	40.0	40.0	40.0	40.0
34	39.29154793608224	40.0	40.0	40.0	40.0	40.0
35	39.298966225985346	40.0	40.0	40.0	40.0	40.0
36	39.2709352011122	40.0	40.0	40.0	40.0	40.0
37	39.2828926669541	40.0	40.0	40.0	40.0	40.0
38	39.26354918023235	40.0	40.0	40.0	40.0	40.0
39	38.05535930212859	40.0	40.0	40.0	40.0	40.0
40	39.256439238773744	40.0	40.0	40.0	40.0	40.0
41	39.26746090294105	40.0	40.0	40.0	40.0	40.0
42	39.23140564761689	40.0	40.0	40.0	40.0	40.0
43	39.25786624669131	40.0	40.0	40.0	40.0	40.0
44	39.198598807301046	40.0	40.0	40.0	40.0	40.0
45	39.04847255474753	40.0	40.0	40.0	40.0	40.0
46	39.0713728049221	40.0	40.0	40.0	40.0	40.0
47	39.164192857610246	40.0	40.0	40.0	40.0	40.0
48	39.23890281735464	40.0	40.0	40.0	40.0	40.0
49	39.21764828736641	40.0	40.0	40.0	40.0	40.0
50	39.20466179822718	40.0	40.0	40.0	40.0	40.0
51	39.17084386184914	40.0	40.0	40.0	40.0	40.0
52	39.18297342914843	40.0	40.0	40.0	40.0	40.0
53	39.10671097084045	40.0	40.0	40.0	40.0	40.0
54	39.04315175135642	40.0	40.0	40.0	40.0	40.0
55	39.08566798222694	40.0	40.0	40.0	40.0	40.0
56	39.08758261094045	40.0	40.0	40.0	40.0	40.0
57	39.02936212208268	40.0	40.0	40.0	40.0	40.0
58	39.004593854005975	40.0	40.0	40.0	40.0	40.0
59	38.96801512341557	40.0	40.0	40.0	40.0	40.0
60	38.9522965236402	40.0	40.0	40.0	40.0	40.0
61	38.86791302781142	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.059646733512835226
1101	2	-0.02723988423510093
1101	3	0.03344235157739206
1101	4	0.12054695068778898
1101	5	-0.08820687133504634
1101	6	-0.03480840375297589
1101	7	-0.08302532133709661
1101	8	-0.08782999945173486
1101	9	-0.1316716665000257
1101	10	-0.01370377706775372
1101	11	0.006281510279130487
1101	12	0.12626268304441624
1101	13	-0.015781928995025396
1101	14	-0.010736379807177343
1101	15	0.024211599162917707
1101	16	0.07054794067340708
1101	17	-0.09201381422749932
1101	18	-0.02083077166297187
1101	19	-0.04139741193078095
1101	20	0.02538672787989782
1101	21	-0.04544828883295082
1101	22	-0.03191772729876874
1101	23	1.4355842694158127E-5
1101	24	0.062165711667233836
1101	25	0.02196152839216836
1101	26	0.02100218045576696
1101	27	0.02514520721879876
1101	28	0.028007854245700514
1101	29	0.10690430789901484
1101	30	-0.0018917936414908354
1101	31	0.1566246238455662
1101	32	-0.013182004877982934
1101	33	0.11000738001169452
1101	34	0.07150321519824843
1101	35	0.04046336939854456
1101	36	-0.0023726516127879904
1101	37	-0.035042142407029075
1101	38	0.04008610928732281
1101	39	1.1564019904929523
1101	40	-0.12876389830867652
1101	41	-0.05444762646133938
1101	42	-0.001923713976808017
1101	43	0.017652924722519003
1101	44	-0.003807811603536493
1101	45	0.09553080932570168
1101	46	0.026808686658263525
1101	47	0.043079622935771056
1101	48	0.03577212087437687
1101	49	0.01895706806990205
1101	50	0.05772652152803204
1101	51	-0.01463669036499482
1101	52	0.117125630902855
1101	53	-0.08407671133463879
1101	54	-0.022132770001519475
1101	55	-0.01311480017685085
1101	56	-0.025796516736349417
1101	57	0.1490614537670183
1101	58	-0.09076694795662377
1101	59	0.03820867361061886
1101	60	-0.09820753654753389
1101	61	0.010353783645911108
1102	1	0.06845316618561981
1102	2	0.09512114072228428
1102	3	0.20792685108226294
1102	4	0.02339509989310784
1102	5	-0.1407604146584589
1102	6	-0.1861540230950709
1102	7	-0.045577604911400726
1102	8	-0.09554859382687653
1102	9	-0.046682613560264485
1102	10	0.017419042091276538
1102	11	0.0747659364315183
1102	12	-0.13067255468937589
1102	13	-0.07586653948538924
1102	14	-0.14939042559507243
1102	15	-0.13836651724108862
1102	16	-0.09461632901496131
1102	17	-0.02039181550000535
1102	18	-0.048187419390878006
1102	19	-0.07349996507544887
1102	20	0.022998626289897572
1102	21	0.0842098726668894
1102	22	-0.09325379487234642
1102	23	-0.09516431325629782
1102	24	0.013682912026339977
1102	25	0.03425444716705783
1102	26	-0.04603720185257032
1102	27	-0.03616598429655227
1102	28	0.04712319145183841
1102	29	0.0029054815386828636
1102	30	-0.01687611056247107
1102	31	0.010841673832089782
1102	32	-0.03846213959140243
1102	33	0.04555032390182845
1102	34	0.06578267857702258
1102	35	-0.019674820294156348
1102	36	-0.019702972179068468
1102	37	0.00975038967272468
1102	38	-0.006668442154293075
1102	39	1.285782369342563
1102	40	0.03889818276494594
1102	41	-0.04492775033135388
1102	42	-0.042129483627697084
1102	43	0.10328046315682826
1102	44	0.05884891952295135
1102	45	-0.012832808482372116
1102	46	0.10720523654033798
1102	47	0.01978893503303425
1102	48	-0.029128558273903593
1102	49	-0.07434824249014582
1102	50	0.030116967208130063
1102	51	-0.07545067963958729
1102	52	-0.02574363105958355
1102	53	-0.11595569887906976
1102	54	0.05005276712201123
1102	55	0.0185383893005735
1102	56	0.027297921391628677
1102	57	0.03068809283614371
1102	58	0.047793972074821056
1102	59	0.019816974559894618
1102	60	0.02662148041761725
1102	61	0.11369594585375609
1103	1	-0.030137627828949576
1103	2	0.0644776529168567
1103	3	0.05436190971838073
1103	4	-0.1147110908853648
1103	5	0.04565001418031045
1103	6	0.0869143495207112
1103	7	-0.05494556442651799
1103	8	0.028668809476435797
1103	9	-0.03926996317187559
1103	10	-0.040840439144112395
1103	11	-0.042034733762776
1103	12	-0.039440393528686
1103	13	-0.04118916666900674
1103	14	0.023376493061824988
1103	15	-0.05646368677724212
1103	16	-0.12339165807635055
1103	17	-0.033795387330961546
1103	18	-0.05612579857434241
1103	19	-0.003618234425879052
1103	20	-0.08126997590039764
1103	21	-0.036327429070901474
1103	22	0.005087011017444354
1103	23	-0.076650117361865
1103	24	-0.04795046672725789
1103	25	0.07640225657151944
1103	26	-0.02062697675726355
1103	27	0.012088318949878385
1103	28	-0.043140108697613755
1103	29	-0.02290265179041029
1103	30	0.09301471199189137
1103	31	-0.10230810195196227
1103	32	0.022239122636641184
1103	33	0.03152712070129127
1103	34	0.10891253932091871
1103	35	0.029454755444433545
1103	36	-0.06254798464510714
1103	37	-0.004827749478174326
1103	38	-0.008970154423771248
1103	39	1.2221852019655373
1103	40	0.013303607130389139
1103	41	9.997162109485203E-4
1103	42	0.07102845759761323
1103	43	-0.04358096498669539
1103	44	0.07514274350508288
1103	45	-0.022477764889515583
1103	46	-0.06036437875353329
1103	47	-0.055806092056208456
1103	48	-0.10192692173104234
1103	49	-0.06830173323591282
1103	50	0.026811847829776525
1103	51	0.14146740802977575
1103	52	0.015615271096841354
1103	53	0.14398264418802142
1103	54	0.07285915862115644
1103	55	0.13432277611322974
1103	56	-0.08239221974096012
1103	57	0.03810137133540081
1103	58	0.025808835533332797
1103	59	-0.12080825484778046
1103	60	0.03565779739533781
1103	61	0.14305730235339098
1104	1	-0.021653997840886063
1104	2	0.12377552183983909
1104	3	-0.02097011526942083
1104	4	0.06976039844386861
1104	5	0.07797525681809958
1104	6	0.05133362810907727
1104	7	0.03605491374116099
1104	8	0.13170594347354125
1104	9	0.02170164646852868
1104	10	0.09108153029131216
1104	11	-0.03536838710536472
1104	12	0.043710319138078546
1104	13	-0.10302510900087469
1104	14	0.0055633624666597825
1104	15	-0.013081201253044128
1104	16	0.00631763197695534
1104	17	0.06151198463610541
1104	18	-0.04849696254031244
1104	19	-0.09467748352415839
1104	20	-0.018137996083140706
1104	21	0.05005537707795327
1104	22	-0.05278204734916869
1104	23	-0.05790403670599886
1104	24	-0.053662324239446946
1104	25	-0.12231887567045874
1104	26	-0.037349921974758615
1104	27	0.04186241087745657
1104	28	0.025484337670988566
1104	29	0.09893379517144041
1104	30	0.0184513246200666
1104	31	-0.00914879211346431
1104	32	-0.017831574792765537
1104	33	0.02360412231303144
1104	34	-0.03327834250766415
1104	35	-0.04278518011062715
1104	36	-0.09917119774727468
1104	37	-0.04929457405710025
1104	38	0.11403801873569819
1104	39	1.2409666682602563
1104	40	-0.08435675596934544
1104	41	-0.05118917786783328
1104	42	-0.022228085822412424
1104	43	-0.00924458725761923
1104	44	-0.04025444029038283
1104	45	-0.07213243182449247
1104	46	-0.031269691091800667
1104	47	0.005299367609389094
1104	48	-0.09297097038967905
1104	49	0.04927551361368643
1104	50	0.07333257796781112
1104	51	0.09474944141781805
1104	52	-0.06975458267739043
1104	53	0.12395529570471098
1104	54	0.018662206463154973
1104	55	0.022326921463893257
1104	56	0.012124975525615866
1104	57	-0.006085445144833557
1104	58	-0.12250776193133106
1104	59	0.012728530523858694
1104	60	-0.10042983098529845
1104	61	0.05352978986533685
1201	1	0.05939706998105976
1201	2	0.058031560727584974
1201	3	0.14885968323260101
1201	4	0.1010516578538585
1201	5	-0.017094410722833686
1201	6	0.10917805375351008
1201	7	0.08281993024645828
1201	8	-0.032148709128755115
1201	9	0.006388856750284333
1201	10	0.04028157772139451
1201	11	0.019162998154079958
1201	12	-0.0026409129074522752
1201	13	0.03802255515973485
1201	14	-0.0042000462080054035
1201	15	0.06141628255652165
1201	16	0.014261249159673639
1201	17	0.12487537954867634
1201	18	0.04258016110782137
1201	19	-0.0246351712216466
1201	20	0.0341459896902947
1201	21	0.04690975505270245
1201	22	0.0538124543247136
1201	23	0.04425956144324772
1201	24	0.0906114456349556
1201	25	0.06742940698580213
1201	26	0.0748001045357114
1201	27	0.026527196314702906
1201	28	0.06556490737262521
1201	29	0.04922344429327552
1201	30	0.08957064383865543
1201	31	0.04868733941039949
1201	32	0.06992820781526632
1201	33	-0.021646449213314156
1201	34	0.07000158283295832
1201	35	0.06936331045158539
1201	36	0.06397906898550332
1201	37	0.12603299762714215
1201	38	0.04217315023437607
1201	39	1.3209705265622702
1201	40	0.11537334720337356
1201	41	0.07981461346683716
1201	42	0.17306128375683727
1201	43	0.004261312934005446
1201	44	0.06859698374868373
1201	45	-0.010891410210014385
1201	46	0.0831569179215137
1201	47	0.06427210084159896
1201	48	0.02474963325027346
1201	49	0.04282668127226685
1201	50	0.021440873378587355
1201	51	0.09162808737498551
1201	52	0.11779944430246303
1201	53	0.1563100456861264
1201	54	0.07101623147699598
1201	55	0.13340915674782394
1201	56	0.19303633349432658
1201	57	0.046272266144818275
1201	58	0.10390483431886821
1201	59	0.17483066616775034
1201	60	0.13706954695565798
1201	61	0.23781531895957642
1202	1	0.12831551512178407
1202	2	0.06622640332354734
1202	3	0.009699291648708197
1202	4	-0.020300397559473993
1202	5	-0.00325412298407457
1202	6	-9.7666143950903E-5
1202	7	0.10537776993137982
1202	8	0.017041217988897017
1202	9	-0.03750399581321773
1202	10	0.02295328701396926
1202	11	-0.05242424193671269
1202	12	-0.054280483785483113
1202	13	-0.07557480164364705
1202	14	-0.04364971582761257
1202	15	-0.021227349908208737
1202	16	-0.0606982796161617
1202	17	-0.07528240009986575
1202	18	-0.07117987094841283
1202	19	-0.04750270775586074
1202	20	-0.1117190777865531
1202	21	-0.008116223494575081
1202	22	0.015370450469461616
1202	23	-0.027406867732899798
1202	24	0.02223136350083621
1202	25	-0.137161119835703
1202	26	-0.06552817258987176
1202	27	-0.05728861877387459
1202	28	0.05371815881738229
1202	29	-0.03239103699634427
1202	30	0.02343854953826252
1202	31	0.08783038152510869
1202	32	-0.06929234291873598
1202	33	0.026393394560123795
1202	34	-0.0027572260127968207
1202	35	-0.07429121519313497
1202	36	-0.1295151639849479
1202	37	-0.15943813023952202
1202	38	-0.12802261024903316
1202	39	1.1042183364507636
1202	40	-0.20441274404716125
1202	41	-0.12142511008038781
1202	42	-0.16335298074498894
1202	43	-0.06290607123028025
1202	44	-0.026259876991865383
1202	45	-0.17319680706128082
1202	46	0.04264249273183651
1202	47	-0.11585882050883356
1202	48	0.02294178940255165
1202	49	0.15068190858445973
1202	50	-0.12767309513868952
1202	51	-0.028705252634168232
1202	52	-0.08785276874472459
1202	53	-0.008333577724044972
1202	54	-0.002706658442733101
1202	55	-0.002406570250570894
1202	56	-0.08681513172243882
1202	57	-0.06399822827054891
1202	58	0.015783342915220544
1202	59	0.008042798764343217
1202	60	-0.07733213921937931
1202	61	0.02452381465163711
1203	1	0.07466135457202938
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2403	8	0.15184457822264363
2403	9	0.09647256187406583
2403	10	0.07385264661677127
2403	11	0.04112828695228643
2403	12	0.16311416101410003
2403	13	0.12952176633548618
2403	14	0.13040998163011608
2403	15	0.0869374555972442
2403	16	0.05935690464682608
2403	17	0.07981004934667624
2403	18	0.12398203056382329
2403	19	0.11727151151742277
2403	20	0.13931505682338496
2403	21	0.09850578214688
2403	22	-4.7469726771964815E-4
2403	23	0.10094827997251343
2403	24	0.10668971617416645
2403	25	0.0025636220512552654
2403	26	0.13780919943111059
2403	27	0.10278061494034318
2403	28	0.07961431851183676
2403	29	0.025845584148108003
2403	30	0.03258747657293526
2403	31	0.06125570275231951
2403	32	-0.00121980232022878
2403	33	0.10116997740670541
2403	34	0.0755520882503049
2403	35	0.06210578495083041
2403	36	0.06289224989514963
2403	37	0.15010051345070252
2403	38	0.037046220574119104
2403	39	1.229966302298962
2403	40	0.10139730368952371
2403	41	-0.024613929767603793
2403	42	0.12044300108262718
2403	43	-7.84423220849817E-4
2403	44	0.132572574828842
2403	45	0.07469339212659776
2403	46	0.0741092223619404
2403	47	0.010591454952155743
2403	48	0.16286467711066166
2403	49	0.09876998472785914
2403	50	0.08184297198614132
2403	51	0.05896775526909437
2403	52	0.08192561020573663
2403	53	0.07082991449502885
2403	54	0.06760414778018742
2403	55	-0.020985674062764303
2403	56	0.01742065072419763
2403	57	0.007910780430840703
2403	58	0.04615375257436938
2403	59	0.09199861713469204
2403	60	-0.07255936693202614
2403	61	-0.0321051529801224
2404	1	-0.09207115867211257
2404	2	-0.11827315551797568
2404	3	-0.06220406566389158
2404	4	0.0836365393194427
2404	5	0.03782417137489347
2404	6	-0.11952956995207842
2404	7	0.0051855278802790394
2404	8	-0.05229343774648498
2404	9	-0.03866807456483201
2404	10	0.07847131010877462
2404	11	0.018401014225013057
2404	12	0.02606735518201475
2404	13	0.031018102467179176
2404	14	-0.11313668394183907
2404	15	-0.047010969726208884
2404	16	-0.06354004862944862
2404	17	-0.14255391543093054
2404	18	-0.06511120479267163
2404	19	0.032213662765741446
2404	20	-0.13802403743826375
2404	21	0.005645022826421098
2404	22	-0.08462085516642048
2404	23	-0.08436203701538858
2404	24	-0.1696268475210374
2404	25	-0.06727839714727679
2404	26	0.010932963602513723
2404	27	0.03195677482321457
2404	28	-0.09001867888137838
2404	29	-0.09525740783995928
2404	30	-0.09564654574552378
2404	31	-0.06343384342063274
2404	32	-0.05091567730397628
2404	33	-0.08687130696557688
2404	34	0.005135002513732445
2404	35	-0.10818506666763028
2404	36	0.007815859438309758
2404	37	-0.04325115632905607
2404	38	-0.08348170487591489
2404	39	1.1772168616289989
2404	40	-0.12353068470651607
2404	41	-0.041064390573453124
2404	42	0.0096648652423994
2404	43	-0.09552056312519852
2404	44	-0.06755810656171946
2404	45	-0.056773797265634585
2404	46	0.023897121675695132
2404	47	-0.08536221131083721
2404	48	0.02580384526545032
2404	49	-0.04665342981951426
2404	50	-0.06545719153664464
2404	51	-0.1527754386935598
2404	52	-0.014015398435653026
2404	53	-0.027519463228927066
2404	54	-0.082173146499251
2404	55	-0.07956035745117873
2404	56	-0.12878411538347478
2404	57	-0.07617516824079473
2404	58	-0.1668897554341413
2404	59	-0.22345462834790197
2404	60	-0.07215158625782436
2404	61	-0.15418175553764257
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	8.0
17	28.0
18	92.0
19	109.0
20	192.0
21	221.0
22	341.0
23	489.0
24	772.0
25	1014.0
26	1408.0
27	1727.0
28	2335.0
29	3012.0
30	3734.0
31	4585.0
32	6362.0
33	7954.0
34	9960.0
35	13110.0
36	19342.0
37	28485.0
38	76519.0
39	510561.0
40	423260.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.25663912744561	16.258119917068612	21.87212323898528	33.6131177165005
2	26.21576159272928	17.796798617014026	27.960454348192542	28.026985442064152
3	27.231136049909466	19.297520661157026	19.759326652444383	33.712016636489125
4	28.397547195687423	21.248255455929925	19.42281473726292	30.93138261111973
5	28.13509247316069	20.82248362122979	19.978827935293435	31.063595970316083
6	28.808170516689806	23.807457909092783	22.188538939299278	25.19583263491813
7	27.131167305025635	24.063369190791498	22.33410808867886	26.47135541550401
8	25.028481894837046	25.624024646362876	23.148363108976973	26.1991303498231
9	25.20587188659948	25.26798975637784	23.428565794297526	26.09757256272516
10	25.89580153116515	23.620028665649	23.40122676070099	27.082943042484857
11	24.91437504313741	24.3416895164218	23.41816799791327	27.32576744252753
12	26.606437132323613	23.500454007230054	22.852832637607214	27.040276222839115
13	25.590948897519855	22.908675975084726	23.029146995260934	28.47122813213448
14	25.62796863809484	23.521787417052924	23.194705011827494	27.655538933024747
15	26.25829022580249	22.92463121436402	22.596383538854145	28.22069502097935
16	26.084127136366202	23.782987233119492	22.391564877319446	27.74132075319486
17	25.827140220558775	22.994099250551937	22.81455799057207	28.364202538317222
18	27.032388239375198	23.13285605057632	22.53596875641459	27.298786953633897
19	25.958188309470685	23.15965726711849	23.397910222199116	27.4842442012117
20	25.684260156450982	23.132407869697687	22.799947293928675	28.383384679922663
21	26.08807112809816	23.258167424241748	23.269282310031812	27.384479137628283
22	26.940331886904247	23.046357141000392	22.453951655624984	27.559359316470378
23	25.912025678971624	23.94755925175306	22.66710648150223	27.473308587773086
24	26.3205873679323	24.129072507598906	22.308292870069675	27.24204725439912
25	26.26519221133342	22.97599274305521	22.33634899307202	28.422466052539345
26	26.033482697080817	23.29088462838186	23.377114629430604	27.298518045106718
27	26.4492152800996	23.53379866460025	22.138342680892524	27.87864337440762
28	26.191152730183457	23.636611358158373	22.168729344463756	28.003506567194414
29	26.196261992199858	24.599393521635037	22.425985168798366	26.77835931736674
30	27.411369990346184	24.037374699830856	22.013838032808632	26.537417277014324
31	26.36056510230625	23.77931214991471	23.074323627827013	26.785799119952024
32	26.27693455035357	23.779939603144797	22.310444138287107	27.63268170821453
33	26.632341987108525	24.88192674752447	22.00953549637377	26.476195768993232
34	26.664162829491378	24.039794876575467	22.038129436430474	27.25791285750268
35	26.66846536592624	24.13167195669497	21.955126337707878	27.24473633967091
36	26.944992968042015	25.425659789480477	21.499864201193773	26.129483041283734
37	27.67158380847976	24.45561709576998	21.668290575383576	26.20450852036668
38	26.613966571084624	24.791663118568046	22.586523559524245	26.007846750823084
39	27.36971360200906	24.687148058903265	21.275966202559033	26.667172136528645
40	27.07021470553172	25.764932714604694	21.167672533951944	25.997180045911648
41	26.88144091945204	25.082891053502937	21.446889221339504	26.58877880570552
42	27.175447575834443	26.15009936170079	20.90781726052127	25.766635801943494
43	27.198932253874748	26.264833666630516	20.786808423290708	25.749425656204032
44	27.10355936290192	25.448696286642146	21.836537677221923	25.611206673234012
45	27.31357692262874	25.637380436546103	20.557519085782715	26.49152355504244
46	27.224478563956755	25.648047141457536	21.530250864765005	25.597223429820705
47	28.041422669526657	25.76815961693084	20.49907629920914	25.691341414333362
48	28.210207588419365	25.920541115665625	20.272924227851576	25.596327068063434
49	28.0075401951021	26.2654611198606	20.04919233323862	25.677806351798683
50	27.986654966157865	26.14248028676405	20.19359621233376	25.677268534744325
51	28.192728534152728	26.352587482666607	19.843208401419478	25.61147558176119
52	28.059080996144747	26.565831944719577	19.715745759536617	25.65934129959906
53	27.729040597120346	26.728611239838617	19.792474325958366	25.749873837082664
54	27.94380887416067	26.74071212356168	19.529571422552998	25.785907579724654
55	27.658228018296537	26.892466169066374	19.503039114538	25.946266698099084
56	27.507459970724824	26.975110723086065	19.60011509284963	25.917314213339477
57	27.752883819863555	26.87740729154435	19.156684931531405	26.213023957060688
58	27.379549147963335	27.090024300367237	19.36015905043021	26.17026750123922
59	27.20475860529696	27.013385370121213	19.305570619412865	26.47628540516896
60	27.386809678197167	27.168366317952064	18.9746338586312	26.47019014521957
61	27.46398642549755	27.212288044057974	19.00959196716448	26.314133563280002
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	2.0
2	3.5
3	4.0
4	2.0
5	0.0
6	1.0
7	5.0
8	8.0
9	8.5
10	9.0
11	9.5
12	13.0
13	16.0
14	24.5
15	33.0
16	36.0
17	53.0
18	67.0
19	88.0
20	154.0
21	199.0
22	270.0
23	341.0
24	498.0
25	874.5
26	1094.0
27	1398.0
28	1702.0
29	2102.0
30	3283.5
31	4065.0
32	4983.0
33	5901.0
34	7139.5
35	10096.0
36	11814.0
37	14040.0
38	16266.0
39	18821.0
40	24353.5
41	27331.0
42	31004.5
43	38614.5
44	42551.0
45	47086.5
46	51622.0
47	55168.0
48	61960.5
49	65207.0
50	68852.0
51	72497.0
52	75133.0
53	80174.5
54	82580.0
55	82441.0
56	82302.0
57	80431.5
58	75990.5
59	73420.0
60	69981.5
61	65532.5
62	64522.0
63	54861.5
64	45201.0
65	40291.0
66	30984.0
67	26587.0
68	23061.5
69	19536.0
70	16526.0
71	11239.0
72	8962.0
73	7340.5
74	5719.0
75	4610.0
76	2805.0
77	2109.0
78	1632.5
79	1156.0
80	847.0
81	392.0
82	246.0
83	198.5
84	102.0
85	53.0
86	44.0
87	35.0
88	22.5
89	10.0
90	10.0
91	7.0
92	4.0
93	4.0
94	4.0
95	4.0
96	4.5
97	5.0
98	3.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03164157003139955
3	8.963617572634434E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.058296679607143
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1115621.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.40915995414598
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.32379560921152	63.72615862345875
2	13.24495962217699	20.50562395958607
3	2.8067662319612956	6.518082486113627
4	0.7283227192635753	2.255153994948506
5	0.3035851556580934	1.175013593702083
6	0.14326371543972885	0.6653954318459534
7	0.09450857976159634	0.5121080837463202
8	0.07017760819366746	0.43459117582903917
9	0.051779588465950835	0.36073930013285765
>10	0.23119704156912835	2.8857508905606855
>50	0.00126471407571007	0.06030667977666682
>100	2.5294281514201403E-4	0.0415097925735499
>500	0.0	0.0
>1k	0.0	0.0
>5k	1.2647140757100702E-4	0.8595659877258681
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTCACAATCTGGGGGGGGGGGGGGG	8780	0.7870056228773034	TruSeq Adapter, Index 16 (97% over 38bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.8693812683698138	0.0	0.0	0.0	0.0
2	0.8723392621687831	0.0	0.0	0.0	0.0
3	0.8736838048046783	0.0	0.0	0.0	8.963617572634434E-5
4	0.8757454368463842	0.0	0.0	0.0	8.963617572634434E-5
5	0.8771796156580057	0.0	0.0	0.0	8.963617572634434E-5
6	0.8816614244443229	0.0	0.0	0.0	6.274532300844104E-4
7	0.887667048217988	0.0	0.0	0.0	6.274532300844104E-4
8	0.9274655102404848	0.0	0.0	0.0	6.274532300844104E-4
9	0.9334711340141499	0.0	0.0	0.0	6.274532300844104E-4
10	0.9373254895703828	0.0	0.0	0.0	6.274532300844104E-4
11	0.9439585665741322	0.0	0.0	0.0	6.274532300844104E-4
12	0.9570454482301786	0.0	0.0	0.0	6.274532300844104E-4
13	0.9799026730403964	0.0	0.0	0.0	6.274532300844104E-4
14	1.010020428084448	0.0	0.0	0.0	6.274532300844104E-4
15	1.0508048880399348	0.0	0.0	0.0	6.274532300844104E-4
16	1.0963400653089177	0.0	0.0	0.0	6.274532300844104E-4
17	1.1510181325019877	0.0	0.0	0.0	6.274532300844104E-4
18	1.2183349004724724	0.0	0.0	0.0	6.274532300844104E-4
19	1.3128114296880393	0.0	0.0	0.0	6.274532300844104E-4
20	1.425215194048875	0.0	0.0	0.0	6.274532300844104E-4
21	1.6016191878783206	0.0	0.0	0.0	6.274532300844104E-4
22	1.7668186597419733	0.0	0.0	0.0	6.274532300844104E-4
23	1.9744160427241868	0.0	0.0	0.0	7.170894058107548E-4
24	2.2167026257124958	0.0	0.0	0.0	8.067255815370991E-4
25	2.510978190622084	0.0	0.0	0.0	8.067255815370991E-4
26	2.83375805941265	0.0	0.0	0.0	8.067255815370991E-4
27	3.2009078351877562	0.0	0.0	0.0	8.963617572634434E-4
28	3.610007341202792	0.0	0.0	0.0	8.963617572634434E-4
29	4.0526307769394805	0.0	0.0	0.0	8.963617572634434E-4
30	4.545719379610101	0.0	0.0	0.0	8.963617572634434E-4
31	5.109082744050175	0.0	0.0	0.0	8.963617572634434E-4
32	5.6832024495774105	0.0	0.0	0.0	8.963617572634434E-4
33	6.335484900338018	0.0	0.0	0.0	8.963617572634434E-4
34	7.04970594852553	0.0	0.0	0.0	8.963617572634434E-4
35	7.796733837028883	0.0	0.0	0.0	8.963617572634434E-4
36	8.602742329160172	0.0	0.0	0.0	8.963617572634434E-4
37	9.428022599072625	0.0	0.0	0.0	8.963617572634434E-4
38	10.323487994578803	0.0	0.0	0.0	8.963617572634434E-4
39	11.27838217459155	0.0	0.0	0.0	9.859979329897878E-4
40	12.298800399060255	0.0	0.0	0.0	0.001075634108716132
41	13.368428884002721	0.0	0.0	0.0	0.001075634108716132
42	14.497217244924576	0.0	0.0	0.0	0.001075634108716132
43	15.669389514897981	0.0	0.0	0.0	0.001075634108716132
44	16.836183614327805	0.0	0.0	0.0	0.001075634108716132
45	18.059807049168132	0.0	0.0	0.0	0.001075634108716132
46	19.281817032845385	0.0	0.0	0.0	0.001075634108716132
47	20.50965336794485	0.0	0.0	0.0	0.001075634108716132
48	21.785176148530727	0.0	0.0	0.0	0.001075634108716132
49	23.053259126531323	0.0	0.0	0.0	0.001075634108716132
50	24.34500605492367	0.0	0.0	0.0	0.001075634108716132
>>END_MODULE