##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-27C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 565156 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.58746965439631 40.0 40.0 40.0 40.0 40.0 2 39.28786742067677 40.0 40.0 40.0 40.0 40.0 3 39.254768594865844 40.0 40.0 40.0 40.0 40.0 4 39.143287870959526 40.0 40.0 40.0 40.0 40.0 5 39.186872297206435 40.0 40.0 40.0 40.0 40.0 6 39.333281430259966 40.0 40.0 40.0 40.0 40.0 7 39.35510903184254 40.0 40.0 40.0 40.0 40.0 8 39.366765990275255 40.0 40.0 40.0 40.0 40.0 9 39.3771489641798 40.0 40.0 40.0 40.0 40.0 10-11 39.38651982815364 40.0 40.0 40.0 40.0 40.0 12-13 39.29389407526418 40.0 40.0 40.0 40.0 40.0 14-15 39.337450190743795 40.0 40.0 40.0 40.0 40.0 16-17 39.443796049232425 40.0 40.0 40.0 40.0 40.0 18-19 39.48390532879417 40.0 40.0 40.0 40.0 40.0 20-21 39.47096376929556 40.0 40.0 40.0 40.0 40.0 22-23 39.509303625901524 40.0 40.0 40.0 40.0 40.0 24-25 39.4945855657553 40.0 40.0 40.0 40.0 40.0 26-27 39.466317264613664 40.0 40.0 40.0 40.0 40.0 28-29 39.48425213569351 40.0 40.0 40.0 40.0 40.0 30-31 39.45938167548467 40.0 40.0 40.0 40.0 40.0 32-33 39.46821428275686 40.0 40.0 40.0 40.0 40.0 34-35 39.4686703153625 40.0 40.0 40.0 40.0 40.0 36-37 39.45335885079576 40.0 40.0 40.0 40.0 40.0 38-39 39.39158156629168 40.0 40.0 40.0 40.0 40.0 40-41 39.436745584465626 40.0 40.0 40.0 40.0 40.0 42-43 39.42286754889075 40.0 40.0 40.0 40.0 40.0 44-45 39.319163230010545 40.0 40.0 40.0 40.0 40.0 46-47 39.30972823859014 40.0 40.0 40.0 40.0 40.0 48-49 39.3938255646117 40.0 40.0 40.0 40.0 40.0 50-51 39.34695415058472 40.0 40.0 40.0 40.0 40.0 52-53 39.30502127055536 40.0 40.0 40.0 40.0 40.0 54-55 39.228346749772555 40.0 40.0 40.0 40.0 40.0 56-57 39.240907302590365 40.0 40.0 40.0 40.0 40.0 58-59 39.18932510749724 40.0 40.0 40.0 40.0 40.0 60-61 39.11907765754582 40.0 40.0 40.0 40.0 40.0 62-63 39.40594765750361 40.0 40.0 40.0 40.0 40.0 64-65 39.400635831201186 40.0 40.0 40.0 40.0 40.0 66-67 39.401620236752386 40.0 40.0 40.0 40.0 40.0 68-69 39.419717342435675 40.0 40.0 40.0 40.0 40.0 70-71 39.40589999443681 40.0 40.0 40.0 40.0 40.0 72-73 39.41444525075755 40.0 40.0 40.0 40.0 40.0 74-75 39.42168832247073 40.0 40.0 40.0 40.0 40.0 76-77 39.418805255757846 40.0 40.0 40.0 40.0 40.0 78-79 39.40397248822029 40.0 40.0 40.0 40.0 40.0 80-81 39.41828797013798 40.0 40.0 40.0 40.0 40.0 82-83 39.398501880192626 40.0 40.0 40.0 40.0 40.0 84-85 39.41245944147884 40.0 40.0 40.0 40.0 40.0 86-87 39.39530249446895 40.0 40.0 40.0 40.0 40.0 88-89 39.4376937242055 40.0 40.0 40.0 40.0 40.0 90-91 39.49496878155671 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09589339153754395 1101 2 0.10321239654437875 1101 3 0.04185025342831494 1101 4 0.0886009926284288 1101 5 0.0010956112524809214 1101 6 0.14056645348029662 1101 7 0.0652410178595133 1101 8 -0.0016874050779662753 1101 9 0.0012816147580920756 1101 10-11 -0.013921080977247868 1101 12-13 -0.06780922178188575 1101 14-15 0.0675612114282842 1101 16-17 0.005762834906605008 1101 18-19 0.12480611517546691 1101 20-21 -0.013643770915329867 1101 22-23 0.05616176191340827 1101 24-25 -0.015974587551419006 1101 26-27 0.09193393891491297 1101 28-29 0.08538791470549967 1101 30-31 0.027353970942584738 1101 32-33 -0.020103087998151636 1101 34-35 0.06508935446024111 1101 36-37 -0.019772346643364358 1101 38-39 -0.12101886826349073 1101 40-41 -0.06711441243401595 1101 42-43 -0.12150075997718801 1101 44-45 -0.12768628540118243 1101 46-47 -0.03187868088027557 1101 48-49 -0.057690734234085994 1101 50-51 -0.09028744514142062 1101 52-53 0.07325708129083353 1101 54-55 -0.06022469123850982 1101 56-57 -0.17466713370311737 1101 58-59 -0.06744641270176999 1101 60-61 -0.09673061788873838 1101 62-63 -0.02065736952734909 1101 64-65 0.02410144364070277 1101 66-67 -0.018646179231936344 1101 68-69 0.06685759534646252 1101 70-71 0.04321378652353758 1101 72-73 -0.11869012611614238 1101 74-75 -0.0488852550811103 1101 76-77 0.1494487432410736 1101 78-79 0.026564671982917787 1101 80-81 0.2065122444167855 1101 82-83 -0.1864586435587583 1101 84-85 -0.27636190647538683 1101 86-87 0.10881304164448835 1101 88-89 0.28372387102730556 1101 90-91 0.12653569216207217 1102 1 0.10632752030581827 1102 2 0.11728250503150406 1102 3 -0.04247082864473839 1102 4 -0.1637167727719131 1102 5 -0.07942468748839815 1102 6 0.05775263778738804 1102 7 -0.14099868727935672 1102 8 -0.04592515832403876 1102 9 0.05849421914444264 1102 10-11 0.028910691442696645 1102 12-13 -5.461720395203429E-4 1102 14-15 -0.10075563725196446 1102 16-17 -0.06339030627601971 1102 18-19 0.07776927555942592 1102 20-21 -0.10218279546678133 1102 22-23 -0.021270185782043427 1102 24-25 0.01461536646664996 1102 26-27 -0.06332050670547318 1102 28-29 -0.12464055683539499 1102 30-31 -0.013509599991209598 1102 32-33 0.021971825507804965 1102 34-35 -0.03806695237592095 1102 36-37 -0.15033599064706493 1102 38-39 0.09200722673506334 1102 40-41 0.046306444561381 1102 42-43 0.09481372133186028 1102 44-45 -0.09161436932784284 1102 46-47 -0.035877945139340284 1102 48-49 -0.1426090527019852 1102 50-51 -0.0038831262464000815 1102 52-53 -0.01696317491263244 1102 54-55 0.027226720261815274 1102 56-57 0.059013051165145214 1102 58-59 0.07778917400754892 1102 60-61 0.3024943827868327 1102 62-63 0.1155463762751694 1102 64-65 0.18018478045241437 1102 66-67 0.08158310450791362 1102 68-69 0.10824320646261043 1102 70-71 0.07573645277049224 1102 72-73 0.08860833782861022 1102 74-75 0.09889379656009822 1102 76-77 0.27321994239291314 1102 78-79 0.051014250814425566 1102 80-81 0.21737449987313084 1102 82-83 -0.0722911065880183 1102 84-85 -0.18433456734845777 1102 86-87 -0.07282869979785289 1102 88-89 0.26818529544425473 1102 90-91 0.5507781164044943 1103 1 -0.06432132402723312 1103 2 0.13635240199614174 1103 3 0.16152063148707185 1103 4 0.06830030696446698 1103 5 0.01696443346212817 1103 6 -0.00761077073391192 1103 7 0.062458102844104246 1103 8 0.11186190191916268 1103 9 -0.1109961735038425 1103 10-11 0.003120289511940655 1103 12-13 0.1389358663898861 1103 14-15 0.04883314952981266 1103 16-17 0.0039335589425917306 1103 18-19 0.0012817604754218337 1103 20-21 -0.020296598492869578 1103 22-23 -0.05150131917107359 1103 24-25 -0.06047606581775966 1103 26-27 -0.044577352523717195 1103 28-29 -0.007139396527222175 1103 30-31 0.05655444902021145 1103 32-33 -0.0011780545056581104 1103 34-35 0.11460184220535297 1103 36-37 -0.06709531019323833 1103 38-39 0.07221072809652895 1103 40-41 -0.03248699789648413 1103 42-43 0.09852928368392355 1103 44-45 -0.007583844995515676 1103 46-47 0.0854476887439688 1103 48-49 -0.12996443664974322 1103 50-51 0.04574169936894634 1103 52-53 -0.07350252407675839 1103 54-55 -0.06974823377729678 1103 56-57 -0.18406058295584415 1103 58-59 0.03262305612614114 1103 60-61 0.0927584130146002 1103 62-63 0.008083552841021913 1103 64-65 0.15185744429370374 1103 66-67 0.10928899022535887 1103 68-69 0.047940177867118905 1103 70-71 -0.014092877238113033 1103 72-73 0.10779265706180041 1103 74-75 0.012310364753005842 1103 76-77 0.22679674772350467 1103 78-79 0.1430678978925286 1103 80-81 0.3633301938209428 1103 82-83 0.011871742785807271 1103 84-85 0.46857970764732215 1103 86-87 0.2437591176358609 1103 88-89 0.1648939685354307 1103 90-91 0.5507781164044943 1104 1 -0.11394903686544211 1104 2 0.05978676345673506 1104 3 -0.06093557148131623 1104 4 -0.13594822817725571 1104 5 -0.1401437231463447 1104 6 -0.07950076012546248 1104 7 -0.012961426616648453 1104 8 -0.05453251426913397 1104 9 0.038667429601360936 1104 10-11 0.02442681420384929 1104 12-13 -0.056614930235241445 1104 14-15 0.06884921092000695 1104 16-17 -0.04343445509626065 1104 18-19 0.018275107887255615 1104 20-21 -0.048740725675280316 1104 22-23 -0.077694472259914 1104 24-25 -0.015030659672788715 1104 26-27 -0.12012061417566144 1104 28-29 -0.04236579272801322 1104 30-31 -0.015352989782918769 1104 32-33 -0.05846592994515731 1104 34-35 -0.09791787468078894 1104 36-37 -0.039480527949194766 1104 38-39 0.03740906079472239 1104 40-41 -0.05883104479973156 1104 42-43 -0.09436447262940817 1104 44-45 0.0025866136120455963 1104 46-47 -0.07487918195690924 1104 48-49 -0.04055594978763111 1104 50-51 -0.143183483513873 1104 52-53 -0.07845655610338298 1104 54-55 0.08507064773729667 1104 56-57 -0.02538613534688494 1104 58-59 -0.12048409065072008 1104 60-61 -0.11561710332228614 1104 62-63 0.10223461127529987 1104 64-65 0.06560266794269154 1104 66-67 -0.01483660841828538 1104 68-69 -0.1731463377809277 1104 70-71 -0.13045221254029116 1104 72-73 -0.03333147525879809 1104 74-75 0.06143297064659947 1104 76-77 0.1732392799440916 1104 78-79 0.09339693079582645 1104 80-81 0.1014421219289261 1104 82-83 0.08321070931164343 1104 84-85 0.04691160141239692 1104 86-87 -0.05519628236483243 1104 88-89 0.2524155709453879 1104 90-91 0.19522256084893996 1201 1 0.029747180567582632 1201 2 0.03262642037542207 1201 3 0.06678378938180884 1201 4 0.10398953002211186 1201 5 -0.030320445401699203 1201 6 0.07362565366040741 1201 7 0.07017740108971537 1201 8 -0.024111364580420513 1201 9 0.13702387662146975 1201 10-11 0.03508353094886729 1201 12-13 -0.04148184455414139 1201 14-15 -0.014475121146887204 1201 16-17 0.05370799503280921 1201 18-19 -0.015844388229680817 1201 20-21 0.11860064461490794 1201 22-23 0.022032515415389753 1201 24-25 0.015914911625849015 1201 26-27 0.03321713369182078 1201 28-29 -0.011533715658380572 1201 30-31 0.08061459939614934 1201 32-33 0.0863305191942203 1201 34-35 0.09690610500307173 1201 36-37 0.10487297368387516 1201 38-39 0.22778454240696533 1201 40-41 0.01690323465990673 1201 42-43 0.13681615416479076 1201 44-45 -0.09416286197768642 1201 46-47 0.006437837397065493 1201 48-49 0.06419838179533599 1201 50-51 -0.00806440736896974 1201 52-53 -0.04330513240761036 1201 54-55 0.020241286197510533 1201 56-57 0.06417399341635388 1201 58-59 0.018488181443316876 1201 60-61 0.05922065142909361 1201 62-63 -0.07977834444034926 1201 64-65 -0.10138341999017086 1201 66-67 -0.06176180073331494 1201 68-69 0.027642316536365286 1201 70-71 -0.0258060260089934 1201 72-73 -0.028770345854852053 1201 74-75 -0.15585182455642865 1201 76-77 -0.11329913993633767 1201 78-79 -0.12569628054416881 1201 80-81 -0.07869973900907468 1201 82-83 -0.21715755495592504 1201 84-85 -0.19559304033329283 1201 86-87 -0.26079068795924343 1201 88-89 0.16165631476470566 1201 90-91 0.050778116404494256 1202 1 0.14030272575810443 1202 2 0.12390921794005294 1202 3 -0.12473719670624206 1202 4 0.13150248113438323 1202 5 -0.02451809834298757 1202 6 0.02716334404020415 1202 7 0.06847960822044996 1202 8 0.03205883759996908 1202 9 -0.014278697431528542 1202 10-11 0.017343772122522694 1202 12-13 0.09876019976260864 1202 14-15 -0.13427213228514745 1202 16-17 0.002789756508462915 1202 18-19 0.01666243714642235 1202 20-21 -0.12787783446119505 1202 22-23 0.02170912629743782 1202 24-25 -0.10576024400516104 1202 26-27 0.00965637074226322 1202 28-29 -0.13408886283152555 1202 30-31 0.03690420678932327 1202 32-33 0.005821596482917357 1202 34-35 0.041602411777418524 1202 36-37 -0.1733639599285226 1202 38-39 -0.07901309684778113 1202 40-41 -0.20481551565380585 1202 42-43 -0.21833921973155412 1202 44-45 -0.16145792318503283 1202 46-47 -0.11561384975400557 1202 48-49 -0.06346258349249467 1202 50-51 -0.10623119727115693 1202 52-53 -0.1113048842387272 1202 54-55 -0.1214136881959007 1202 56-57 -0.1946516012990429 1202 58-59 -0.05034091957107023 1202 60-61 -0.13130033989413192 1202 62-63 -0.14032251651148187 1202 64-65 -0.239443799160604 1202 66-67 -0.1694220898279113 1202 68-69 0.062096683500705296 1202 70-71 -0.11274351753088041 1202 72-73 -0.0475788752704247 1202 74-75 -0.025648529356431027 1202 76-77 -0.3437852367555294 1202 78-79 -0.3237724748494841 1202 80-81 -0.1924086421148985 1202 82-83 -0.23874793655416937 1202 84-85 0.22624937425737812 1202 86-87 0.10937529064047169 1202 88-89 -0.4724945291171565 1202 90-91 -0.2867218835955043 1203 1 -6.678400347652769E-4 1203 2 -0.0309222818887136 1203 3 0.0470734967009534 1203 4 -5.939626077662297E-4 1203 5 -0.02866376131861159 1203 6 -0.0015198371853486492 1203 7 0.009899587912705954 1203 8 0.03697355109860467 1203 9 -0.046783098443306415 1203 10-11 -0.07937634756096656 1203 12-13 -0.011066823951786375 1203 14-15 -0.05194166887213214 1203 16-17 -9.413401696036772E-4 1203 18-19 0.034641997225492105 1203 20-21 -0.09969934264137237 1203 22-23 -0.1509847735697747 1203 24-25 -0.05338970585004432 1203 26-27 0.05141941906627068 1203 28-29 0.09835966185318767 1203 30-31 -0.04505975598382861 1203 32-33 -0.11086171281083779 1203 34-35 0.016013825390231773 1203 36-37 -0.11410726251639858 1203 38-39 -0.05406133309318051 1203 40-41 -0.0755686350180298 1203 42-43 -0.11347577302184675 1203 44-45 -0.0951738484787299 1203 46-47 -0.01261957709825623 1203 48-49 -0.17389483692119256 1203 50-51 -0.08533011839768534 1203 52-53 0.03628110414262409 1203 54-55 -0.07528900421556983 1203 56-57 -0.1466344837815612 1203 58-59 -0.11699127907938589 1203 60-61 -0.20277073007975588 1203 62-63 -0.11948723949696927 1203 64-65 -0.20449767655862416 1203 66-67 -0.2951104227210877 1203 68-69 -0.11421516057725256 1203 70-71 -0.16396380171426017 1203 72-73 -0.1644743601460803 1203 74-75 -0.2126246965623153 1203 76-77 -0.3102059637947363 1203 78-79 -0.06485574172968711 1203 80-81 -0.5291330741881737 1203 82-83 -0.18358722674313555 1203 84-85 -0.020011367122549473 1203 86-87 0.2795332083279618 1203 88-89 0.4008676302119909 1203 90-91 -0.41473912497481535 1204 1 0.07310152208344789 1204 2 -0.05912783044035308 1204 3 -0.1995966614456819 1204 4 -0.25290029790300395 1204 5 -0.2550509354975219 1204 6 -0.01413268766925313 1204 7 -0.21846061095657632 1204 8 -0.15825288975344876 1204 9 -0.08049715784005684 1204 10-11 -0.1920563679295455 1204 12-13 -0.18703596352442986 1204 14-15 -0.08537210596963973 1204 16-17 -0.07443215762565103 1204 18-19 -0.16423889156410354 1204 20-21 -0.08124213188896334 1204 22-23 -0.0545872845338522 1204 24-25 -0.12077792892369388 1204 26-27 -0.08026909836525675 1204 28-29 -0.12986053930828234 1204 30-31 -0.1108797145793261 1204 32-33 -0.1636437681280114 1204 34-35 -0.09408062720164168 1204 36-37 0.04165850859460818 1204 38-39 0.004111150809130493 1204 40-41 -0.10342520176175896 1204 42-43 -0.1224243882284668 1204 44-45 0.010518103448561078 1204 46-47 -0.11471476934843139 1204 48-49 0.018433025677389026 1204 50-51 -0.0498121079916487 1204 52-53 -0.07198394051946622 1204 54-55 -0.03902332061387881 1204 56-57 -0.058581513911740046 1204 58-59 -0.28201482140801204 1204 60-61 -0.27320251022947417 1204 62-63 -0.21645187997327042 1204 64-65 -0.11477595988979061 1204 66-67 -0.02489678225433778 1204 68-69 -0.08640636760532061 1204 70-71 -0.0861470362417407 1204 72-73 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2401 36-37 0.04575623347314206 2401 38-39 0.03009568044499389 2401 40-41 0.03522338374389733 2401 42-43 0.011262311829433713 2401 44-45 0.014428565221486167 2401 46-47 0.0922394149005541 2401 48-49 0.13677653350295316 2401 50-51 0.18865870442838428 2401 52-53 0.1417465118337944 2401 54-55 0.11802065227070813 2401 56-57 0.0745483095658912 2401 58-59 0.12480826601277784 2401 60-61 0.03903803743882861 2401 62-63 0.05145023853589947 2401 64-65 -0.027383162555828733 2401 66-67 -0.07753421393243087 2401 68-69 6.090696747449442E-4 2401 70-71 -0.02959640179253853 2401 72-73 -0.06939308400487931 2401 74-75 -0.12935569496600152 2401 76-77 -0.02012238587588655 2401 78-79 0.09086661959654663 2401 80-81 -0.0596491959590395 2401 82-83 0.0024662640908559297 2401 84-85 0.10231030519636874 2401 86-87 -0.31884929601784506 2401 88-89 -0.22464460539480058 2401 90-91 -0.5899626243362448 2402 1 0.01781995213794829 2402 2 0.04211727899608064 2402 3 0.0818014131224345 2402 4 0.17074007171498806 2402 5 0.0871345466601241 2402 6 0.060874652651911276 2402 7 0.0037064428175455078 2402 8 0.11511618636866672 2402 9 0.017791532813745903 2402 10-11 0.06503390489169192 2402 12-13 0.08143001677247952 2402 14-15 0.05741026962087403 2402 16-17 0.03973132027819304 2402 18-19 -0.016678145930697497 2402 20-21 0.0597982601677316 2402 22-23 0.08805663947055109 2402 24-25 0.10574855949052875 2402 26-27 0.01853401154382084 2402 28-29 -0.006873159886488622 2402 30-31 0.003983779318062375 2402 32-33 0.035327833829668975 2402 34-35 0.0023605002412452336 2402 36-37 0.04805371052371754 2402 38-39 -0.3285242643417874 2402 40-41 0.019146525289805538 2402 42-43 0.09834497192675684 2402 44-45 0.09001003134509489 2402 46-47 0.16361800222263412 2402 48-49 0.07068339942354385 2402 50-51 0.08862705290717798 2402 52-53 0.17579557679169255 2402 54-55 0.05684642070465884 2402 56-57 0.13634692921394986 2402 58-59 0.14302028394496347 2402 60-61 0.3057573446101074 2402 62-63 0.17611182246722734 2402 64-65 0.07665723911484434 2402 66-67 0.10768487423754891 2402 68-69 0.14456220559471689 2402 70-71 0.0622862871800578 2402 72-73 0.10064601730579881 2402 74-75 0.08893259173040491 2402 76-77 0.08823451542316008 2402 78-79 0.014615593155021145 2402 80-81 0.13557109508221288 2402 82-83 0.2833217294445092 2402 84-85 -0.15945082298127033 2402 86-87 -0.1477535850061713 2402 88-89 -0.4849287631103749 2402 90-91 -1.4492218835955057 2403 1 -0.0548999210574479 2403 2 0.08909457022217993 2403 3 0.18453417708126096 2403 4 0.03916471137726063 2403 5 0.09067853298267892 2403 6 -0.028028775668047956 2403 7 -0.035137205073787925 2403 8 -0.14502214593262863 2403 9 0.10956520060592112 2403 10-11 0.11644896800191873 2403 12-13 0.10391096297788494 2403 14-15 0.11270132436573022 2403 16-17 0.0257238117005798 2403 18-19 0.05467281386647471 2403 20-21 0.021719879652643215 2403 22-23 0.018287816424518155 2403 24-25 0.03304718512484328 2403 26-27 0.11511542781421724 2403 28-29 0.04584947839713749 2403 30-31 0.012203101443056141 2403 32-33 0.015116120743762451 2403 34-35 0.05498097963491233 2403 36-37 -0.011955024980466078 2403 38-39 0.040083886310959826 2403 40-41 0.09843750242838212 2403 42-43 0.0259059908004744 2403 44-45 0.12420971950222537 2403 46-47 0.04593397001931265 2403 48-49 0.10806419290592117 2403 50-51 0.2105711449092098 2403 52-53 0.14279443463075125 2403 54-55 0.09533763940160611 2403 56-57 0.06795975314290814 2403 58-59 -0.10399609152440803 2403 60-61 0.057106074094797066 2403 62-63 0.01220396894698439 2403 64-65 0.10194314537293536 2403 66-67 0.10290365855968986 2403 68-69 0.025398990182395664 2403 70-71 0.08835332390556516 2403 72-73 0.12946532485568696 2403 74-75 0.10256082132216449 2403 76-77 -0.06069584395487482 2403 78-79 0.028189540825728443 2403 80-81 0.2269342238224823 2403 82-83 -0.16073722966111603 2403 84-85 0.01853519337313969 2403 86-87 -8.910693793424684E-4 2403 88-89 0.3499818335079823 2403 90-91 -0.13969807407169554 2404 1 -0.07727286123126476 2404 2 -0.047091145965680425 2404 3 -0.20475052813957006 2404 4 -0.048617902179657335 2404 5 0.008822261864807501 2404 6 -0.18137230635591095 2404 7 -0.15011585697238417 2404 8 0.038928294367615024 2404 9 -0.09709963814551514 2404 10-11 0.05042504086985389 2404 12-13 0.038010907258943405 2404 14-15 0.005321047014376745 2404 16-17 0.049407376376535694 2404 18-19 0.01752391625245764 2404 20-21 -0.0013628854727727457 2404 22-23 0.024334782711541436 2404 24-25 -0.03675829964151944 2404 26-27 -0.014177099399219628 2404 28-29 0.061360313434285274 2404 30-31 0.04396150486580552 2404 32-33 -0.02219717247493236 2404 34-35 0.05423288733487652 2404 36-37 -0.030638286115532765 2404 38-39 0.07843069419397608 2404 40-41 0.1133559087519771 2404 42-43 0.037239839782508 2404 44-45 0.06368672027412714 2404 46-47 0.1090337597151958 2404 48-49 0.06543058452025008 2404 50-51 0.13177260819990977 2404 52-53 0.08623668919162242 2404 54-55 0.006541654148527698 2404 56-57 0.025599015963599925 2404 58-59 -0.010100349548586962 2404 60-61 0.0769948761392456 2404 62-63 0.06706813969802283 2404 64-65 0.020096532016921742 2404 66-67 0.018773244649558762 2404 68-69 0.02535943102009952 2404 70-71 0.08778305595687641 2404 72-73 0.21569456828303402 2404 74-75 0.18231153580363468 2404 76-77 -0.08544053997365353 2404 78-79 -0.011692994845198257 2404 80-81 -0.081768266220962 2404 82-83 0.1688888816878844 2404 84-85 -0.02239836069866641 2404 86-87 -0.036934084792292765 2404 88-89 -0.29547735435267697 2404 90-91 0.012316577942954154 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 14.0 31 306.0 32 2270.0 33 4160.0 34 5567.0 35 6900.0 36 10147.0 37 15374.0 38 39024.0 39 253250.0 40 228144.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.31289060011749 15.029478586443389 23.07132897819363 33.58630183524549 2 25.97679098256934 17.77505623505634 28.102661940149265 28.14549084222505 3 27.11092158625229 18.678205663569 20.20769486655012 34.003177883628595 4 28.143202938657648 21.35162680746555 19.03474438915981 31.470425864717 5 27.927864164938534 20.990310639894115 20.332970011819747 30.748855183347608 6 27.773393540898443 23.96931112825485 22.721867944425963 25.53542738642074 7 26.096157521109216 24.160054922888545 22.870853357303115 26.872934198699124 8 24.86163112485756 24.83809779954561 23.622150344329707 26.678120731267118 9 24.873663200956905 24.508985129769478 23.98187403124093 26.635477638032683 10-11 24.794569994833285 23.556416281522267 23.86456128927234 27.784452434372103 12-13 25.347514668516304 23.236239197672855 23.430167953626963 27.986078180183878 14-15 25.62796820700833 22.805827063677995 23.390798292860733 28.175406436452942 16-17 25.711484970521415 22.86802228057386 23.07522170869636 28.34527104020837 18-19 25.8924084677505 22.98887386845402 22.973126004147527 28.145591659647955 20-21 25.745900247011445 22.975603196285626 23.116272321270586 28.162224235432344 22-23 25.698214298353022 22.843869657227387 23.08628060217002 28.37163544224957 24-25 25.97176708731748 22.885981923575084 22.967375379541224 28.174875609566207 26-27 25.977960067662735 23.00993000162787 22.952246813269255 28.05986311744014 28-29 25.920984648486435 22.953485409338306 23.106540495013768 28.01898944716149 30-31 26.206443210953495 22.965197035139155 22.97422114011604 27.854138613791307 32-33 26.17546139102845 22.894488432024236 23.06174492308355 27.868305253863767 34-35 26.14831232260444 22.855153484429554 23.166507350850125 27.830026842115885 36-37 26.362857081022895 22.95104488454 23.001574219312367 27.68452381512474 38-39 26.532738513486663 22.92873501279806 23.061319232368383 27.47720724134689 40-41 26.367827022698265 22.864736354763632 23.22060111614405 27.546835506394054 42-43 26.53627772055621 23.005943998162444 23.034629843464668 27.423148437816675 44-45 26.592183006677534 22.97050078798152 23.14466907483929 27.292647130501656 46-47 26.571519496977857 22.868277192199624 23.164319131851915 27.395884178970604 48-49 26.668916566352035 23.055213251275372 22.996509882723977 27.279360299648616 50-51 26.86978077698346 22.88833523218109 23.122582099082294 27.119301891753157 52-53 26.70219574472596 22.991915853857297 23.148537075635332 27.157351325781416 54-55 26.96864837763347 23.081278176110462 22.935653192834803 27.014420253421275 56-57 26.940621431576417 23.02076446901857 22.969078670593184 27.069535428811825 58-59 27.056757509372126 22.958292622859783 22.917085843700754 27.067864024067333 60-61 27.408665482460243 23.054456182630947 22.746458634146002 26.790419700762808 62-63 27.30328855379628 22.931902284898182 22.827269034734872 26.93754012657067 64-65 27.310691331441063 22.938892695181234 22.830617271867148 26.919798701510555 66-67 27.597050192291373 23.018014195167925 22.53717409850857 26.847761514032133 68-69 27.57991205055246 22.935343606420062 22.584038530234164 26.90070581279332 70-71 27.541794679603864 22.870729584510503 22.673596525186632 26.913879210698997 72-73 27.927393412379832 22.782881705278317 22.29463515931361 26.995089723028244 74-75 28.08195840256287 22.94989830722809 22.073830006217484 26.894313283991554 76-77 28.041894501143904 22.85862634064026 22.023413541886388 27.07606561632944 78-79 28.67807702948949 22.72675233979221 21.567589226938377 27.02758140377992 80-81 28.695147794425907 22.60189401988204 21.63593239417207 27.06702579151998 82-83 29.182754412383936 22.46265620873984 21.13662153771491 27.217967841161318 84-85 30.18990634755463 21.970231901293296 20.195666716218224 27.64419503493385 86-87 31.031748095374294 20.741568943550273 19.33097584440573 28.8957071166697 88-89 30.736549867496738 20.946031211879024 18.668237075674085 29.64918184495015 90-91 28.443711916891417 20.7371890919988 18.63450204774748 32.1845969433623 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 1.5 10 3.0 11 5.5 12 8.5 13 13.5 14 20.0 15 23.0 16 28.0 17 43.5 18 60.5 19 80.5 20 111.0 21 161.5 22 242.0 23 360.5 24 494.5 25 633.0 26 822.5 27 1078.0 28 1389.5 29 1759.0 30 2234.5 31 2789.0 32 3455.5 33 4156.0 34 5003.5 35 6127.5 36 7406.5 37 8703.0 38 10275.0 39 12092.0 40 13947.5 41 15978.0 42 18076.5 43 20320.0 44 22523.5 45 24636.0 46 26811.5 47 28798.5 48 30615.0 49 32230.0 50 33572.5 51 34753.5 52 35747.0 53 36549.5 54 37120.0 55 37294.5 56 37234.5 57 37077.0 58 36595.5 59 35928.5 60 35129.5 61 34145.0 62 32906.0 63 31196.5 64 28932.0 65 26525.0 66 24119.5 67 21829.5 68 19337.5 69 16657.0 70 14138.0 71 11740.5 72 9617.0 73 7634.0 74 5872.0 75 4515.0 76 3441.0 77 2526.5 78 1847.0 79 1351.5 80 992.0 81 711.5 82 528.0 83 387.0 84 256.5 85 165.0 86 97.5 87 61.5 88 44.5 89 30.0 90 21.0 91 16.0 92 10.5 93 7.0 94 7.5 95 8.5 96 6.0 97 4.0 98 3.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.020702248582692213 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.06382838590700234 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 1.871789880355191E-4 64-65 2.0390810269627682E-4 66-67 2.2333442767202892E-4 68-69 0.0 70-71 2.7455782462344395E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.770195816538269E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.00499425660490436 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 5061.0 32-33 11816.0 34-35 13370.0 36-37 14694.0 38-39 16352.0 40-41 17983.0 42-43 19300.0 44-45 20051.0 46-47 20493.0 48-49 21219.0 50-51 21965.0 52-53 22221.0 54-55 22440.0 56-57 22091.0 58-59 21810.0 60-61 21760.0 62-63 21952.0 64-65 21392.0 66-67 20987.0 68-69 20954.0 70-71 20452.0 72-73 19641.0 74-75 19241.0 76-77 18571.0 78-79 17928.0 80-81 17212.0 82-83 16364.0 84-85 15628.0 86-87 14907.0 88-89 13806.0 90-91 13495.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.79258141750688 #Duplication Level Percentage of deduplicated Percentage of total 1 99.80077045208823 99.5937651086993 2 0.19287295243412098 0.384945796180339 3 0.0048078369119711846 0.014393593694399378 4 0.0013104920848968935 0.005231095523162864 5 0.0 0.0 6 0.0 0.0 7 2.3826648078559772E-4 0.001664405902800172 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0010616537734713955 0.0 0.0 0.0 0.0 2 0.0010616537734713955 0.0 0.0 0.0 0.0 3 0.0010616537734713955 0.0 0.0 0.0 1.769422955785659E-4 4 0.0012385960690499615 0.0 0.0 0.0 1.769422955785659E-4 5 0.0012385960690499615 0.0 0.0 0.0 1.769422955785659E-4 6 0.0014155383646285273 0.0 0.0 0.0 3.538845911571318E-4 7 0.002477192138099923 0.0 0.0 0.0 3.538845911571318E-4 8 0.007962403301035465 0.0 0.0 0.0 3.538845911571318E-4 9 0.007962403301035465 0.0 0.0 0.0 3.538845911571318E-4 10-11 0.007962403301035465 0.0 0.0 0.0 3.538845911571318E-4 12-13 0.007962403301035465 0.0 0.0 0.0 3.538845911571318E-4 14-15 0.007962403301035465 0.0 0.0 0.0 3.538845911571318E-4 16-17 0.007962403301035465 0.0 0.0 0.0 3.538845911571318E-4 18-19 0.007962403301035465 0.0 0.0 0.0 3.538845911571318E-4 20-21 0.007962403301035465 0.0 0.0 0.0 3.538845911571318E-4 22-23 0.008227816744403316 0.0 0.0 0.0 3.538845911571318E-4 24-25 0.008316287892192598 0.0 0.0 0.0 5.308268867356978E-4 26-27 0.008316287892192598 0.0 0.0 0.0 6.192980345249806E-4 28-29 0.008316287892192598 0.0 0.0 0.0 7.077691823142636E-4 30-31 0.008316287892192598 0.0 0.0 0.0 7.077691823142636E-4 32-33 0.008493230187771164 0.0 0.0 0.0 7.077691823142636E-4 34-35 0.008493230187771164 0.0 0.0 0.0 7.077691823142636E-4 36-37 0.008493230187771164 0.0 0.0 0.0 7.077691823142636E-4 38-39 0.00867017248334973 0.0 0.0 0.0 7.077691823142636E-4 40-41 0.00867017248334973 0.0 0.0 0.0 7.077691823142636E-4 42-43 0.00867017248334973 0.0 0.0 0.0 7.077691823142636E-4 44-45 0.008935585926717579 0.0 0.0 0.0 7.077691823142636E-4 46-47 0.009024057074506861 0.0 0.0 0.0 7.077691823142636E-4 48-49 0.009112528222296145 0.0 0.0 0.0 7.077691823142636E-4 50-51 0.009200999370085427 0.0 0.0 0.0 7.077691823142636E-4 52-53 0.009200999370085427 0.0 0.0 0.0 7.077691823142636E-4 54-55 0.009200999370085427 0.0 0.0 0.0 7.077691823142636E-4 56-57 0.009377941665663993 0.0 0.0 0.0 7.077691823142636E-4 58-59 0.00955488396124256 0.0 0.0 0.0 7.077691823142636E-4 60-61 0.009731826256821126 0.0 0.0 0.0 7.077691823142636E-4 62-63 0.009731826256821126 0.0 0.0 0.0 7.077691823142636E-4 64-65 0.009908768552399692 0.0 0.0 0.0 7.077691823142636E-4 66-67 0.009908768552399692 0.0 0.0 0.0 7.077691823142636E-4 68-69 0.009908768552399692 0.0 0.0 0.0 7.077691823142636E-4 70-71 0.009908768552399692 0.0 0.0 0.0 7.077691823142636E-4 72-73 0.009908768552399692 0.0 0.0 0.0 7.077691823142636E-4 74-75 0.010085710847978257 0.0 0.0 0.0 7.077691823142636E-4 76-77 0.010085710847978257 0.0 0.0 0.0 7.077691823142636E-4 78-79 0.010085710847978257 0.0 0.0 0.0 7.077691823142636E-4 80 0.010085710847978257 0.0 0.0 0.0 7.077691823142636E-4 >>END_MODULE