##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-27A_S19_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1297470
Sequences flagged as poor quality	0
Sequence length	61
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.463386436680615	40.0	40.0	40.0	40.0	40.0
2	39.177607189376246	40.0	40.0	40.0	40.0	40.0
3	39.084805043661895	40.0	40.0	40.0	40.0	40.0
4	38.99285532613471	40.0	40.0	40.0	40.0	40.0
5	39.03005079115509	40.0	40.0	40.0	40.0	40.0
6	39.17863842709273	40.0	40.0	40.0	40.0	40.0
7	39.20817899450469	40.0	40.0	40.0	40.0	40.0
8	39.21785628954812	40.0	40.0	40.0	40.0	40.0
9	39.24580606873376	40.0	40.0	40.0	40.0	40.0
10	39.256297255427874	40.0	40.0	40.0	40.0	40.0
11	39.248207665687836	40.0	40.0	40.0	40.0	40.0
12	39.25686143032209	40.0	40.0	40.0	40.0	40.0
13	39.08381080102045	40.0	40.0	40.0	40.0	40.0
14	39.25781097058121	40.0	40.0	40.0	40.0	40.0
15	39.161463463509754	40.0	40.0	40.0	40.0	40.0
16	39.350640862601836	40.0	40.0	40.0	40.0	40.0
17	39.287564259674596	40.0	40.0	40.0	40.0	40.0
18	39.38429713211095	40.0	40.0	40.0	40.0	40.0
19	39.3322049835449	40.0	40.0	40.0	40.0	40.0
20	39.33059261485815	40.0	40.0	40.0	40.0	40.0
21	39.32432503256337	40.0	40.0	40.0	40.0	40.0
22	39.38233639313433	40.0	40.0	40.0	40.0	40.0
23	39.3785628954812	40.0	40.0	40.0	40.0	40.0
24	39.37890509992524	40.0	40.0	40.0	40.0	40.0
25	39.343762861569054	40.0	40.0	40.0	40.0	40.0
26	39.3047916329472	40.0	40.0	40.0	40.0	40.0
27	39.3312585262087	40.0	40.0	40.0	40.0	40.0
28	39.3360709688856	40.0	40.0	40.0	40.0	40.0
29	39.3388455995129	40.0	40.0	40.0	40.0	40.0
30	39.34149074737759	40.0	40.0	40.0	40.0	40.0
31	39.29030189522687	40.0	40.0	40.0	40.0	40.0
32	39.3144812596823	40.0	40.0	40.0	40.0	40.0
33	39.31761196790677	40.0	40.0	40.0	40.0	40.0
34	39.318772688385856	40.0	40.0	40.0	40.0	40.0
35	39.320591612908196	40.0	40.0	40.0	40.0	40.0
36	39.28554802808543	40.0	40.0	40.0	40.0	40.0
37	39.30270141120797	40.0	40.0	40.0	40.0	40.0
38	39.27674320022814	40.0	40.0	40.0	40.0	40.0
39	38.054146916691714	40.0	40.0	40.0	40.0	40.0
40	39.27240861060371	40.0	40.0	40.0	40.0	40.0
41	39.278084271698	40.0	40.0	40.0	40.0	40.0
42	39.23987452503719	40.0	40.0	40.0	40.0	40.0
43	39.27568575766684	40.0	40.0	40.0	40.0	40.0
44	39.2097851973456	40.0	40.0	40.0	40.0	40.0
45	39.05130754468311	40.0	40.0	40.0	40.0	40.0
46	39.074053349981114	40.0	40.0	40.0	40.0	40.0
47	39.13896737496821	40.0	40.0	40.0	40.0	40.0
48	39.23624900768419	40.0	40.0	40.0	40.0	40.0
49	39.21151780002621	40.0	40.0	40.0	40.0	40.0
50	39.19820573886101	40.0	40.0	40.0	40.0	40.0
51	39.153290634850904	40.0	40.0	40.0	40.0	40.0
52	39.16485467872089	40.0	40.0	40.0	40.0	40.0
53	39.07865615389951	40.0	40.0	40.0	40.0	40.0
54	38.99269501414291	40.0	40.0	40.0	40.0	40.0
55	39.04675715045435	40.0	40.0	40.0	40.0	40.0
56	39.021881045419164	40.0	40.0	40.0	40.0	40.0
57	38.95828342851858	40.0	40.0	40.0	40.0	40.0
58	38.89119285995052	40.0	40.0	40.0	40.0	40.0
59	38.82683684401181	40.0	40.0	40.0	40.0	40.0
60	38.81731061219142	40.0	40.0	40.0	40.0	40.0
61	38.73667059739339	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.04409822321390777
1101	2	-0.0057226037999953405
1101	3	-0.0103755300428503
1101	4	-0.08477059762068251
1101	5	-0.03331521473103294
1101	6	0.0026978059719979797
1101	7	-0.11318329273080252
1101	8	-0.007078706086751652
1101	9	-0.03126119548289097
1101	10	-0.06351886807441787
1101	11	-0.07844423118080357
1101	12	-0.025922033275421086
1101	13	-0.011044585263547901
1101	14	-0.006477772600867127
1101	15	-0.02319898255003494
1101	16	0.053779570907998675
1101	17	0.05184107035167784
1101	18	0.03746725029364484
1101	19	-0.02369681644912447
1101	20	0.04096420037247839
1101	21	0.021590212857141466
1101	22	0.015674619410290802
1101	23	0.0498833634172442
1101	24	0.051208065183232065
1101	25	0.09290942460671658
1101	26	0.001170494030944269
1101	27	0.07780911204341834
1101	28	-0.00785858232118386
1101	29	-0.011991314269785391
1101	30	0.11048348773648087
1101	31	0.04769646377103243
1101	32	0.10439334813622025
1101	33	0.0264679415758593
1101	34	-9.911241437023932E-4
1101	35	0.09751613469774156
1101	36	-0.047256859430568454
1101	37	-0.03513663435128933
1101	38	-0.025230813521758932
1101	39	1.1715873945653854
1101	40	-0.01980133177447385
1101	41	-0.055192023269299284
1101	42	-0.054969719355661084
1101	43	-0.07790007481427352
1101	44	0.05788821845240477
1101	45	-0.03485256128012537
1101	46	0.059583614644473926
1101	47	0.08468198900429513
1101	48	-0.04597938690043435
1101	49	-0.013654847329448216
1101	50	0.050470159406920345
1101	51	-0.048454376554069256
1101	52	0.09350002443405714
1101	53	-0.10867792611661287
1101	54	-0.030500667616323085
1101	55	-0.0804280690163992
1101	56	0.048863322000912035
1101	57	-0.025270839506724485
1101	58	0.08427737383320988
1101	59	-0.09481789801890272
1101	60	-0.041572114243976444
1101	61	0.14263567105408015
1102	1	0.06107848638348656
1102	2	0.03908431491317543
1102	3	0.02950651007429883
1102	4	-0.06755706846581688
1102	5	-0.1454024516503054
1102	6	-0.0013516290884396653
1102	7	-0.05346778804324259
1102	8	-0.13500997103696477
1102	9	-0.1464585882913454
1102	10	0.008817953389986144
1102	11	-0.0982483941479515
1102	12	-0.11766558564367102
1102	13	-0.09280443025341611
1102	14	-0.15909587085239707
1102	15	-0.09791551279371191
1102	16	-0.12797664626432237
1102	17	-0.01956507638051619
1102	18	-0.04879922778852119
1102	19	-0.06323165582285384
1102	20	0.02844729962631476
1102	21	-0.07885312505127473
1102	22	-0.015385545153094426
1102	23	-0.02815773603472138
1102	24	-0.0826504955127163
1102	25	-0.052879772217735876
1102	26	-0.13137647109037687
1102	27	-0.05544992854767372
1102	28	-0.0861606055736388
1102	29	-0.07286423228622141
1102	30	-0.11046967016241638
1102	31	-0.053770802384732974
1102	32	-0.11423498335142312
1102	33	0.07779551350083125
1102	34	-0.06612951840654091
1102	35	-0.11052803909704778
1102	36	-0.07659264789717923
1102	37	-0.09204430127856966
1102	38	-0.1153902188945537
1102	39	1.10580924863131
1102	40	-0.16057367552600255
1102	41	-0.07307251272700199
1102	42	-0.09116208298576822
1102	43	-0.1030329436319306
1102	44	-0.14001946103270768
1102	45	-0.10506687692723204
1102	46	-0.03172483217055344
1102	47	-0.034475306187957244
1102	48	-0.03551871453561262
1102	49	-0.06779095467192064
1102	50	-0.07411769959004033
1102	51	-0.009086974913266488
1102	52	0.007079829210965727
1102	53	-0.03083821704898071
1102	54	-0.030071143320881788
1102	55	0.03626370555846137
1102	56	-9.063813505889584E-4
1102	57	-0.08927995928191024
1102	58	0.0657562024946472
1102	59	0.04615234783084787
1102	60	0.02981752126733994
1102	61	-0.02261095309890493
1103	1	0.02647000934582877
1103	2	0.1366440968331588
1103	3	0.0623456432892624
1103	4	-0.07088204585232205
1103	5	-0.047919810510791194
1103	6	0.08234402023563092
1103	7	0.01802633390502706
1103	8	0.09085147561485485
1103	9	0.04943378167560297
1103	10	0.024809866425989924
1103	11	0.027047576790252492
1103	12	0.039455967892415345
1103	13	0.05209368885742549
1103	14	0.08290384656996963
1103	15	0.0324209439449632
1103	16	-0.02893324437921052
1103	17	0.037922750204209876
1103	18	0.0333337963602105
1103	19	0.02079912474687262
1103	20	-0.023670736255219538
1103	21	-0.06799818205475816
1103	22	-0.025855017300990824
1103	23	-0.0011426152182210103
1103	24	-0.050639635209485334
1103	25	0.11845596059782793
1103	26	0.061883790191970434
1103	27	-0.07582045482666899
1103	28	-0.0045416979255392675
1103	29	0.060297301615079846
1103	30	-0.074659801211439
1103	31	-0.016987934163502416
1103	32	0.07917792811583979
1103	33	0.008733773201328177
1103	34	0.06837790071289618
1103	35	-0.012983172614980276
1103	36	0.018137628839539843
1103	37	0.011174491007935217
1103	38	-1.814135175948195E-5
1103	39	1.178485185980648
1103	40	0.01690628549576445
1103	41	0.029337350106317217
1103	42	0.04670830127611936
1103	43	0.06406074541906293
1103	44	0.019898271005047263
1103	45	-0.022626687978927862
1103	46	-0.006479288230458735
1103	47	0.009201057940991575
1103	48	-0.006582998033813681
1103	49	-0.004845010025086083
1103	50	0.0421045579139232
1103	51	-0.03795300835356841
1103	52	0.010430675560101577
1103	53	0.06919752756493125
1103	54	-0.056763018631315276
1103	55	-0.003159661281202375
1103	56	0.03438237948822831
1103	57	0.10364959877541224
1103	58	-0.02527644396140971
1103	59	0.07203226398977591
1103	60	0.08988665082626568
1103	61	0.1037677854104544
1104	1	-0.047166973248749855
1104	2	-0.033799854913496574
1104	3	-0.039380467172030365
1104	4	-0.1478588211646752
1104	5	-0.005085130017548067
1104	6	0.033676108246204706
1104	7	0.0019882060790479272
1104	8	-0.02213165433515485
1104	9	0.002355692955084976
1104	10	0.007761979692169518
1104	11	-0.024316051638024305
1104	12	0.08261955325812664
1104	13	-0.07893800868044565
1104	14	0.014995115926296876
1104	15	0.036365241937900805
1104	16	-0.06775612340250348
1104	17	-0.03614648741384485
1104	18	0.06960504395249245
1104	19	0.039698003374461166
1104	20	0.0039849727349619
1104	21	-0.03515704217708304
1104	22	0.013561892319998492
1104	23	-0.030298996333534944
1104	24	0.07370665762758222
1104	25	-0.04031966599156078
1104	26	-0.0049707223572781345
1104	27	0.03776392160996522
1104	28	-0.02498244907629754
1104	29	0.07867756732273534
1104	30	-0.1620081366106092
1104	31	-0.011041808921270047
1104	32	-0.018991214648195864
1104	33	-0.0594496267625928
1104	34	-0.06227321687699572
1104	35	0.011921886072315147
1104	36	-0.11448784906761489
1104	37	-0.08233109257707838
1104	38	-0.08910410935743585
1104	39	1.1544802512437045
1104	40	-0.058423172206907736
1104	41	-0.01864388858248134
1104	42	-0.046374803651055174
1104	43	-0.046621679817924644
1104	44	-0.11070443029788635
1104	45	-0.09502626200870878
1104	46	-0.11413569553247527
1104	47	0.038546681562557694
1104	48	-0.04326275804731239
1104	49	-0.016216319883504582
1104	50	-0.0846932749916931
1104	51	0.025007950195806927
1104	52	-0.00647266062466656
1104	53	0.04030278061568282
1104	54	-0.05014977281972932
1104	55	-0.09671948041843592
1104	56	-0.029830652174389627
1104	57	0.020958304978165643
1104	58	-0.11662773554733263
1104	59	-0.14392811845233666
1104	60	-0.1582673036554425
1104	61	0.048947280647389846
1201	1	-0.011458434841067344
1201	2	0.02267561802017326
1201	3	0.020432623450801657
1201	4	-0.02046646125091911
1201	5	-0.046458074484633016
1201	6	-0.06687667815253917
1201	7	0.045696298635064636
1201	8	-0.06901574335222449
1201	9	-0.18304405834328463
1201	10	-0.03560893754313099
1201	11	0.0010877401910462936
1201	12	-0.01904437908388701
1201	13	-0.061478736184966465
1201	14	-0.07033462272799795
1201	15	-0.09850000984637575
1201	16	0.012821811816642992
1201	17	-0.08180796757170583
1201	18	-0.028342993429383512
1201	19	0.028081477603883798
1201	20	0.016268882714726374
1201	21	-0.01552563610722757
1201	22	-0.07402706031174233
1201	23	-0.0713689009979106
1201	24	-0.04423295037127417
1201	25	-0.06847798513078374
1201	26	0.02428271993105824
1201	27	0.00913536829411754
1201	28	0.02862494370160107
1201	29	-0.06917450745495302
1201	30	0.020770842876729034
1201	31	0.039255135253220885
1201	32	-0.032145232015508896
1201	33	0.007512416631314522
1201	34	0.041233336794135766
1201	35	-0.04379981889231743
1201	36	-0.047071594291253405
1201	37	-0.08752874767598229
1201	38	-0.039388862275501424
1201	39	1.3161035692857226
1201	40	0.0014052445668681912
1201	41	-0.03397630931318929
1201	42	-2.904610558474019E-4
1201	43	0.10962982231902885
1201	44	0.06379842768093624
1201	45	-0.022189426301565618
1201	46	0.07603954507067812
1201	47	-0.018354440381358472
1201	48	0.06031345994628623
1201	49	0.06873514149982185
1201	50	-0.013384177876140768
1201	51	0.027269950746159566
1201	52	-0.022925980303796223
1201	53	-0.021451854674175763
1201	54	0.04717476887611127
1201	55	0.14156594127712196
1201	56	0.016590865741534344
1201	57	-0.023100199119838294
1201	58	-0.07317949091410014
1201	59	-0.005626325641003405
1201	60	0.12503241664230558
1201	61	0.11901356625021009
1202	1	0.06208663736147457
1202	2	0.07975671552640762
1202	3	-0.014669344077049118
1202	4	0.03189367438350388
1202	5	-0.08242605813115489
1202	6	0.019416234189229442
1202	7	0.12545955993850555
1202	8	0.059060029605142006
1202	9	-0.007474828849012738
1202	10	0.04782556912464031
1202	11	-0.015564078511857815
1202	12	-0.07434579274815434
1202	13	0.011629184340449683
1202	14	0.005012923214366083
1202	15	-0.04913501927310904
1202	16	0.03765796626309026
1202	17	0.03697574248554503
1202	18	0.03296528482836436
1202	19	0.00950425783330644
1202	20	0.0581118257883233
1202	21	0.04725362646692588
1202	22	0.030329953797277653
1202	23	0.04653636208728784
1202	24	-0.0813880805154028
1202	25	-0.03209011488127089
1202	26	-0.04006006775686899
1202	27	0.03946319752181182
1202	28	0.09573635867801755
1202	29	0.042433837791286066
1202	30	0.0012150387041103272
1202	31	0.013607522322374166
1202	32	0.06037760967757322
1202	33	0.07717215655024035
1202	34	0.005102370847772875
1202	35	0.09391124663900285
1202	36	-0.1168162783749267
1202	37	-0.144878489735774
1202	38	-0.16876504061681175
1202	39	1.1471157860521757
1202	40	-0.0920519829384645
1202	41	-0.08534819210405686
1202	42	-0.15483635507592197
1202	43	-0.09765051414273529
1202	44	-0.026768626413989693
1202	45	0.03165826812043093
1202	46	-0.0636770314723023
1202	47	-0.06882508630231143
1202	48	-0.08324770330534648
1202	49	-0.11810265658168362
1202	50	-0.10189668814886232
1202	51	0.009107783357812593
1202	52	-0.11079415800975312
1202	53	0.07886651329289407
1202	54	-0.03201667317194534
1202	55	-0.09233506769380995
1202	56	-0.002695603274567304
1202	57	0.05115205051932037
1202	58	-0.029734348960658963
1202	59	-0.05352102250083135
1202	60	-0.0280072723101199
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2402	58	0.06739286321096927
2402	59	0.06527566533026885
2402	60	0.07087286631475109
2402	61	0.03232492913940632
2403	1	0.03456242180597968
2403	2	0.14299760399681105
2403	3	0.018780526163034494
2403	4	0.08384704799617992
2403	5	0.07693888838102225
2403	6	0.01900210677748504
2403	7	0.06789149046972653
2403	8	0.10668177579880478
2403	9	0.07168598917613878
2403	10	0.0034921398881166965
2403	11	0.09015611085334996
2403	12	0.12056000416376378
2403	13	0.11258182269533279
2403	14	0.07588247453274732
2403	15	0.09388715180075025
2403	16	0.13675938243353158
2403	17	-0.018502799902208267
2403	18	0.07659978324014816
2403	19	0.1224607788143075
2403	20	0.060592231022894794
2403	21	0.03414540407614908
2403	22	0.03998711442349645
2403	23	0.005838535602315176
2403	24	-0.0019206628976959905
2403	25	0.05311551027675421
2403	26	-1.5707528672237459E-4
2403	27	0.06984561875768946
2403	28	0.06131873806522492
2403	29	0.176596588411023
2403	30	0.0325971780427281
2403	31	0.12043495760951828
2403	32	0.07404526734386963
2403	33	0.030929381983767712
2403	34	0.06182184239213484
2403	35	0.08290998827290252
2403	36	0.037278351918274666
2403	37	0.07139046930775095
2403	38	0.08985098916549106
2403	39	1.3071229933222241
2403	40	0.004733225883470027
2403	41	0.08500929430239523
2403	42	0.17139142371901528
2403	43	0.10626291724434367
2403	44	0.10131444318557215
2403	45	0.105666469296267
2403	46	0.141327661971701
2403	47	0.07380620661800208
2403	48	0.20034384210190836
2403	49	0.1670306052601802
2403	50	0.21272825772395976
2403	51	-8.676014900643736E-4
2403	52	0.12668829606570142
2403	53	0.1481173350088696
2403	54	0.007515784245583745
2403	55	0.10678756342474571
2403	56	0.08667309218049724
2403	57	3.5650201965609085E-5
2403	58	0.04233108607655112
2403	59	0.17844845122315434
2403	60	0.06415133412934182
2403	61	0.09056246151826741
2404	1	0.10141002266606591
2404	2	-0.10888495679143517
2404	3	0.0014827474455998413
2404	4	0.03362670201507001
2404	5	0.11363896122963268
2404	6	-0.005522826061614694
2404	7	0.164082297143608
2404	8	0.011407641089896003
2404	9	0.06979902376632907
2404	10	0.13110972987836078
2404	11	0.2021652540816845
2404	12	0.15066157090679866
2404	13	0.05763563695716556
2404	14	0.09643380037490346
2404	15	0.22239792244099732
2404	16	0.0015760569774485589
2404	17	-0.022048967102669792
2404	18	0.03754445072441115
2404	19	0.07019056026081216
2404	20	-0.08700012623098274
2404	21	0.08536310958238147
2404	22	0.05243853272440191
2404	23	0.01697801502966456
2404	24	0.049552382988757415
2404	25	-0.033562845872523894
2404	26	0.0828699134254407
2404	27	-0.0013412365238139046
2404	28	0.08148756401781299
2404	29	0.11260726776708196
2404	30	0.11049999600469818
2404	31	0.06862894786726059
2404	32	0.06473708912343312
2404	33	-0.036786886849824896
2404	34	0.07861660056053665
2404	35	0.0787724527518634
2404	36	0.09526921635014673
2404	37	0.10254863454412799
2404	38	0.07021173916146495
2404	39	1.2211437591339802
2404	40	-0.05951245650221182
2404	41	0.0347547327103328
2404	42	0.08397309114786822
2404	43	0.09175345547888014
2404	44	0.10953385002476068
2404	45	0.0908904321738575
2404	46	0.03684493179064674
2404	47	0.0900233848463472
2404	48	0.10497318872928219
2404	49	0.09977394911098258
2404	50	0.0887329263674701
2404	51	0.08893714227458105
2404	52	0.04315369067766284
2404	53	0.10358392997595445
2404	54	0.09859059106143775
2404	55	0.08283918916417576
2404	56	0.05008639046069874
2404	57	-0.08690008905056601
2404	58	0.08209779689157415
2404	59	0.03123195500895548
2404	60	-0.04386530691391499
2404	61	0.05609253155047611
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	8.0
17	43.0
18	103.0
19	149.0
20	224.0
21	296.0
22	400.0
23	629.0
24	984.0
25	1274.0
26	1649.0
27	2086.0
28	2848.0
29	3558.0
30	4505.0
31	5551.0
32	7651.0
33	9244.0
34	11801.0
35	15118.0
36	23629.0
37	34531.0
38	81637.0
39	585484.0
40	504068.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	28.30161776380186	16.296099331776457	20.272684532204984	35.1295983722167
2	25.273871266434394	18.020879672426144	27.903066263392883	28.80218279774658
3	27.13089869584553	18.758829690728643	19.22843628633902	34.88183532708681
4	28.875041426776725	21.514254664847744	18.15440819440912	31.45629571396641
5	28.56744279251158	20.947459286149197	19.25716972261401	31.227928198725213
6	28.6424348925216	24.331660847649655	21.514408810993704	25.511495448835042
7	26.84362644222988	24.756487625918133	21.891373210941293	26.508512720910694
8	24.98778391793259	26.041064533284008	22.909508505013605	26.0616430437698
9	25.162585647452353	25.346019561145923	23.167934518717196	26.323460272684535
10	25.764295128211057	24.04733828142462	23.121074090345058	27.06729250001927
11	25.214301679422263	24.469082136773874	22.978411832258164	27.338204351545702
12	26.310974434861695	23.973656423655267	22.493853422429805	27.221515719053237
13	25.775162431501307	23.428826870756165	22.735169213931727	28.0608414838108
14	25.738783941054518	23.804635174609047	22.85070175032949	27.60587913400695
15	26.302419323760855	23.275682674743926	22.327529730937904	28.09436827055731
16	26.25948962211072	23.8660624137745	22.251998119417017	27.622449844697762
17	25.95412610696201	23.410252260167866	22.49755292993287	28.138068702937254
18	26.8158801359569	23.46289316901354	22.29916684008108	27.42205985494848
19	26.072047908622164	23.5250911389088	22.748579928630335	27.6542810238387
20	25.87250572267567	23.588522277971748	22.53662897793398	28.002343021418607
21	26.325387099509044	23.443547827695436	22.66819271351168	27.562872359283837
22	26.63907450653965	23.574803270981217	22.025017919489468	27.761104302989665
23	26.042297702451695	24.01489051769983	22.333387284484417	27.609424495364053
24	26.488088356570866	24.142292307336586	22.02363060417582	27.34598873191673
25	26.320377349765312	23.41857615204976	22.138546555989734	28.122499942195194
26	26.233824288808222	23.370790846801853	22.814323259882695	27.581061604507234
27	26.63152134538756	23.60617201168428	21.933994620299508	27.828312022628655
28	26.382498246587588	23.747369881384543	22.06763932884768	27.80249254318019
29	26.21286041295752	24.46075824489198	22.140088017449344	27.18629332470115
30	27.132727538979708	24.05874509622573	21.954110692347413	26.854416672447144
31	26.52639367384217	24.027607574741612	22.464257362405295	26.98174138901092
32	26.24322720371184	24.102753820897593	22.131147540983605	27.52287143440696
33	26.7099252467689	24.542397544758295	21.865724730224766	26.881952478248035
34	26.758306550440476	24.139286457490346	21.83927181360648	27.2631351784627
35	26.559226802931864	24.692671121490285	22.01916036594295	26.728941709634906
36	26.91414830400703	24.524035237808967	21.505160042236042	27.05665641594796
37	26.813028432256626	24.88573916930642	21.77707384371122	26.524158554725734
38	27.08602125675353	24.459833368016216	21.800426984824313	26.653718390405945
39	27.950065023643155	24.575675987385015	21.330538841187206	26.14372014778462
40	26.96779116280145	24.723731569901425	21.48304007029064	26.82543719700648
41	26.84886741119255	25.394190231758728	21.595643829915144	26.161298527133575
42	27.23292253385435	24.95325518123733	21.598803825907343	26.215018459000976
43	27.268607366644314	25.624176281532524	20.986149968785405	26.121066383037757
44	27.028601817383063	25.13877006790138	21.733912922842148	26.098715191873417
45	27.323791686898346	25.312107409034507	20.806646781813836	26.55745412225331
46	27.12979876220645	25.415847765266246	21.486200066282844	25.96815340624446
47	27.66044686967714	25.436657494970984	20.925262241130817	25.977633394221062
48	27.8573685711423	25.63311675799826	20.660670381588783	25.848844289270655
49	27.764495518200803	25.827263828836124	20.49712132072418	25.911119332238897
50	27.749003830531727	25.788650219272892	20.575735855164282	25.8866100950311
51	28.0320161545161	25.823564321333052	20.257115771462924	25.887303752687924
52	27.83047006867211	26.084996184882886	20.31322496859272	25.77130877785228
53	27.7100048556036	26.14673171634026	20.336501036632832	25.80676239142331
54	28.014058128511643	26.286079832288994	19.87175040656046	25.828111632638905
55	27.84758029087378	26.485159579797607	19.96431516721003	25.702944962118586
56	27.5163972962766	26.595605293378654	20.019884852828966	25.868112557515783
57	27.7601794261139	26.514986859041056	19.703962326681925	26.02087138816312
58	27.549384571512253	26.828828412217625	19.611012200667453	26.010774815602673
59	27.207796712062702	26.817961108927374	19.90019037049026	26.074051808519656
60	27.694359021788557	26.86258641818308	19.269193121998967	26.173861438029395
61	27.734514092811395	26.85464789166609	19.38850223897277	26.02233577654975
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	1.5
3	1.0
4	4.5
5	8.0
6	4.5
7	2.0
8	3.0
9	4.5
10	6.0
11	6.5
12	17.0
13	27.0
14	29.0
15	31.0
16	37.0
17	55.5
18	68.0
19	103.0
20	182.0
21	226.0
22	372.5
23	519.0
24	658.0
25	1075.0
26	1353.0
27	1710.0
28	2067.0
29	2776.5
30	4272.0
31	5058.0
32	6267.0
33	7476.0
34	8927.5
35	12372.5
36	14366.0
37	16815.5
38	19265.0
39	22356.5
40	29160.0
41	32872.0
42	36995.5
43	45300.0
44	49481.0
45	53713.5
46	57946.0
47	62631.0
48	70379.5
49	73443.0
50	77329.0
51	81215.0
52	84600.5
53	90140.5
54	92295.0
55	92847.0
56	93399.0
57	91591.5
58	86713.0
59	83642.0
60	80432.0
61	74144.5
62	71067.0
63	63163.0
64	55259.0
65	49446.0
66	38796.5
67	33960.0
68	29621.5
69	25283.0
70	21786.0
71	15291.0
72	12293.0
73	10273.0
74	8253.0
75	6574.0
76	3880.0
77	2865.0
78	2206.5
79	1548.0
80	1172.0
81	612.5
82	429.0
83	317.0
84	148.5
85	92.0
86	67.0
87	42.0
88	32.5
89	17.0
90	11.0
91	8.5
92	6.0
93	5.0
94	5.5
95	7.0
96	7.0
97	7.0
98	6.5
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03229361757882649
3	7.707307298049281E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	7.707307298049281E-5
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.101035091370128
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1297470.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.33193761424364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.60868546660507	61.477404023507255
2	13.504002034691675	20.34565277640025
3	2.8884008115004014	6.527664892106368
4	0.8578233733429022	2.5848598737883015
5	0.359433629587065	1.3538415880256969
6	0.1942829531735076	0.8781426784786616
7	0.1162536475739352	0.6130328768527568
8	0.08566140811773502	0.5162431881818783
9	0.06261712716724148	0.42453625656112165
>10	0.31208738114852047	4.243170379442182
>50	0.009634948438890463	0.4697970121338199
>100	0.0010054967877610916	0.15822502224383217
>500	0.0	0.0
>1k	1.1172186530678794E-4	0.40742943227786776
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGGCATATCTGGGGGGGGGGGGGGG	4841	0.37311074629856567	TruSeq Adapter, Index 5 (97% over 37bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.5418237030528644	0.0	0.0	0.0	7.707307298049281E-5
2	0.5425944337826694	0.0	0.0	0.0	7.707307298049281E-5
3	0.5433651645124743	0.0	0.0	0.0	7.707307298049281E-5
4	0.5442129683152597	0.0	0.0	0.0	7.707307298049281E-5
5	0.5445212606071816	0.0	0.0	0.0	1.5414614596098562E-4
6	0.5474500373804404	0.0	0.0	0.0	1.5414614596098562E-4
7	0.5497622295698552	0.0	0.0	0.0	1.5414614596098562E-4
8	0.5724987860991005	0.0	0.0	0.0	1.5414614596098562E-4
9	0.5754275628723593	0.0	0.0	0.0	1.5414614596098562E-4
10	0.5794353626673449	0.0	0.0	0.0	2.3121921894147843E-4
11	0.5819787740757012	0.0	0.0	0.0	2.3121921894147843E-4
12	0.5893777890818285	0.0	0.0	0.0	2.3121921894147843E-4
13	0.6023260653425513	0.0	0.0	0.0	2.3121921894147843E-4
14	0.6194362875442206	0.0	0.0	0.0	3.85365364902464E-4
15	0.6467972284522956	0.0	0.0	0.0	3.85365364902464E-4
16	0.6742352424333511	0.0	0.0	0.0	3.85365364902464E-4
17	0.7082244676177484	0.0	0.0	0.0	6.165845838439425E-4
18	0.7526956307274927	0.0	0.0	7.707307298049281E-5	6.165845838439425E-4
19	0.8148936006227504	0.0	0.0	7.707307298049281E-5	6.165845838439425E-4
20	0.8864174123486478	0.0	0.0	7.707307298049281E-5	6.936576568244352E-4
21	0.9793675383631221	0.0	0.0	7.707307298049281E-5	6.936576568244352E-4
22	1.0891195942873437	0.0	0.0	7.707307298049281E-5	6.936576568244352E-4
23	1.2215311336678305	0.0	0.0	7.707307298049281E-5	6.936576568244352E-4
24	1.37313386822046	0.0	0.0	7.707307298049281E-5	6.936576568244352E-4
25	1.5471648670104126	0.0	0.0	7.707307298049281E-5	6.936576568244352E-4
26	1.749635829730167	0.0	0.0	7.707307298049281E-5	7.70730729804928E-4
27	1.9895643059184414	0.0	0.0	7.707307298049281E-5	7.70730729804928E-4
28	2.2702644377133963	0.0	0.0	7.707307298049281E-5	7.70730729804928E-4
29	2.558517730660439	0.0	0.0	7.707307298049281E-5	7.70730729804928E-4
30	2.8842285370760017	0.0	0.0	7.707307298049281E-5	8.478038027854209E-4
31	3.2507109990982452	0.0	0.0	7.707307298049281E-5	8.478038027854209E-4
32	3.6555758514647736	0.0	0.0	7.707307298049281E-5	8.478038027854209E-4
33	4.101597724802885	0.0	0.0	7.707307298049281E-5	8.478038027854209E-4
34	4.611744394860768	0.0	0.0	7.707307298049281E-5	8.478038027854209E-4
35	5.1328354412818795	0.0	0.0	7.707307298049281E-5	8.478038027854209E-4
36	5.690844489660647	0.0	0.0	7.707307298049281E-5	8.478038027854209E-4
37	6.291243728178686	0.0	0.0	7.707307298049281E-5	8.478038027854209E-4
38	6.922549268961903	0.0	0.0	7.707307298049281E-5	8.478038027854209E-4
39	7.602179626503888	0.0	0.0	7.707307298049281E-5	8.478038027854209E-4
40	8.35140696894726	0.0	0.0	7.707307298049281E-5	8.478038027854209E-4
41	9.14788010512767	0.0	0.0	7.707307298049281E-5	9.248768757659137E-4
42	9.95483517923343	0.0	0.0	7.707307298049281E-5	0.0010019499487464065
43	10.837090645641132	0.0	0.0	7.707307298049281E-5	0.0010019499487464065
44	11.743084618526826	0.0	0.0	7.707307298049281E-5	0.0010019499487464065
45	12.704648277031454	0.0	0.0	7.707307298049281E-5	0.0010019499487464065
46	13.687638249824658	0.0	0.0	7.707307298049281E-5	0.0010019499487464065
47	14.656909215627337	0.0	0.0	7.707307298049281E-5	0.0010019499487464065
48	15.6554679491626	0.0	0.0	7.707307298049281E-5	0.0010019499487464065
49	16.71121490284939	0.0	0.0	7.707307298049281E-5	0.0010019499487464065
50	17.782761836497183	0.0	0.0	7.707307298049281E-5	0.0010019499487464065
>>END_MODULE