##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-27A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 580266 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.550488913705095 40.0 40.0 40.0 40.0 40.0 2 39.318030006927856 40.0 40.0 40.0 40.0 40.0 3 39.237170539028654 40.0 40.0 40.0 40.0 40.0 4 39.1570555572789 40.0 40.0 40.0 40.0 40.0 5 39.18833086894631 40.0 40.0 40.0 40.0 40.0 6 39.338703284355795 40.0 40.0 40.0 40.0 40.0 7 39.36880671967684 40.0 40.0 40.0 40.0 40.0 8 39.38335177315231 40.0 40.0 40.0 40.0 40.0 9 39.409491509066534 40.0 40.0 40.0 40.0 40.0 10-11 39.41094946110921 40.0 40.0 40.0 40.0 40.0 12-13 39.328111590201736 40.0 40.0 40.0 40.0 40.0 14-15 39.36512909596633 40.0 40.0 40.0 40.0 40.0 16-17 39.4714596409233 40.0 40.0 40.0 40.0 40.0 18-19 39.51365408278272 40.0 40.0 40.0 40.0 40.0 20-21 39.49542106551134 40.0 40.0 40.0 40.0 40.0 22-23 39.53803945087253 40.0 40.0 40.0 40.0 40.0 24-25 39.5205509197506 40.0 40.0 40.0 40.0 40.0 26-27 39.49558995357302 40.0 40.0 40.0 40.0 40.0 28-29 39.517765990080406 40.0 40.0 40.0 40.0 40.0 30-31 39.49718605982653 40.0 40.0 40.0 40.0 40.0 32-33 39.498200514608 40.0 40.0 40.0 40.0 40.0 34-35 39.50742274395273 40.0 40.0 40.0 40.0 40.0 36-37 39.48929700431929 40.0 40.0 40.0 40.0 40.0 38-39 39.41972373379948 40.0 40.0 40.0 40.0 40.0 40-41 39.47443219326925 40.0 40.0 40.0 40.0 40.0 42-43 39.45654602574235 40.0 40.0 40.0 40.0 40.0 44-45 39.34651042132829 40.0 40.0 40.0 40.0 40.0 46-47 39.355399741806856 40.0 40.0 40.0 40.0 40.0 48-49 39.43878762703241 40.0 40.0 40.0 40.0 40.0 50-51 39.404974841242876 40.0 40.0 40.0 40.0 40.0 52-53 39.370551016351186 40.0 40.0 40.0 40.0 40.0 54-55 39.296952955243206 40.0 40.0 40.0 40.0 40.0 56-57 39.3024700744575 40.0 40.0 40.0 40.0 40.0 58-59 39.252052988762536 40.0 40.0 40.0 40.0 40.0 60-61 39.1725361589687 40.0 40.0 40.0 40.0 40.0 62-63 39.33794434288798 40.0 40.0 40.0 40.0 40.0 64-65 39.34600925282467 40.0 40.0 40.0 40.0 40.0 66-67 39.33421562685986 40.0 40.0 40.0 40.0 40.0 68-69 39.35159725693957 40.0 40.0 40.0 40.0 40.0 70-71 39.344412907032684 40.0 40.0 40.0 40.0 40.0 72-73 39.348033703520684 40.0 40.0 40.0 40.0 40.0 74-75 39.342684077421524 40.0 40.0 40.0 40.0 40.0 76-77 39.33927905614914 40.0 40.0 40.0 40.0 40.0 78-79 39.334783193089294 40.0 40.0 40.0 40.0 40.0 80-81 39.32697587037377 40.0 40.0 40.0 40.0 40.0 82-83 39.341010749624346 40.0 40.0 40.0 40.0 40.0 84-85 39.32038015810067 40.0 40.0 40.0 40.0 40.0 86-87 39.307606148682105 40.0 40.0 40.0 40.0 40.0 88-89 39.35794100451996 40.0 40.0 40.0 40.0 40.0 90-91 39.47370467539504 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.015057516047981778 1101 2 -0.009977204596950173 1101 3 0.046103764707886796 1101 4 -0.02571878137091943 1101 5 -0.045989357608370085 1101 6 0.08592718263611943 1101 7 -0.03539166103647062 1101 8 -0.08079435491159614 1101 9 0.13072092140301095 1101 10-11 0.026242759538533278 1101 12-13 -0.09818212817442884 1101 14-15 0.09091300450518247 1101 16-17 0.10401218415928781 1101 18-19 0.04572831910202524 1101 20-21 0.05994653314893128 1101 22-23 -0.03552107809542804 1101 24-25 0.11036397285787558 1101 26-27 0.05295612598642663 1101 28-29 0.13566093235822763 1101 30-31 0.10290433523285714 1101 32-33 0.07053895381066866 1101 34-35 0.01576415801434905 1101 36-37 0.07836016715339866 1101 38-39 0.11568495238821441 1101 40-41 -0.005335746062733904 1101 42-43 0.07421609624945802 1101 44-45 0.008176448846072049 1101 46-47 0.0573279266102702 1101 48-49 0.008378754221993745 1101 50-51 0.0025128684666029244 1101 52-53 0.034646621512258946 1101 54-55 0.01725677350446375 1101 56-57 -0.08133843380382899 1101 58-59 -0.019088347933113425 1101 60-61 -0.038890947413534604 1101 62-63 -0.13257731500699066 1101 64-65 -0.2811976123070252 1101 66-67 -0.02158352857728829 1101 68-69 -0.13237534824672537 1101 70-71 -0.22281474997923567 1101 72-73 -0.07753691504938587 1101 74-75 -0.050707155965589834 1101 76-77 -0.2981012033938697 1101 78-79 -0.5283668636691061 1101 80-81 -0.7030323113694905 1101 82-83 -0.10008381001736666 1101 84-85 -0.29508065096494107 1101 86-87 -0.08475547509811321 1101 88-89 -0.38867756242981955 1101 90-91 0.14464841969754616 1102 1 0.07193725602630963 1102 2 0.07287679586268325 1102 3 -0.07335518010317088 1102 4 -0.05790631215000275 1102 5 -0.07560621961960123 1102 6 0.0072542124138550435 1102 7 0.029479148904762553 1102 8 -0.056853976041644216 1102 9 -0.12479633129733969 1102 10-11 0.015459411431237413 1102 12-13 -0.1112899124089779 1102 14-15 -0.02876426658053788 1102 16-17 0.044384487911869996 1102 18-19 -0.024136088790555732 1102 20-21 0.041296872949970975 1102 22-23 -0.038736876381648244 1102 24-25 -0.04791108541719069 1102 26-27 0.10733250078533274 1102 28-29 -0.016267889993123674 1102 30-31 -0.004338065516769518 1102 32-33 0.014581547521672178 1102 34-35 0.007333129037895958 1102 36-37 -0.033787421938221485 1102 38-39 5.040118519090697E-4 1102 40-41 -0.04884639989938222 1102 42-43 -0.052275952876897236 1102 44-45 0.06548349660761943 1102 46-47 0.009518116270186283 1102 48-49 -0.12159718021812438 1102 50-51 0.04973174004633307 1102 52-53 -0.05399158109022295 1102 54-55 0.01220574967374688 1102 56-57 -0.13125618052925603 1102 58-59 -0.025622078264539994 1102 60-61 0.012459200130237491 1102 62-63 -0.12624939307115568 1102 64-65 -0.14379640683460337 1102 66-67 -0.14017588977235107 1102 68-69 -0.03143874786503886 1102 70-71 -0.03984257452648876 1102 72-73 -0.2268609711885503 1102 74-75 -0.08214037230446536 1102 76-77 -0.07190891293824109 1102 78-79 0.17307297043252845 1102 80-81 0.14245116680361747 1102 82-83 0.021072491929373882 1102 84-85 -0.15964447110204816 1102 86-87 -0.2475526830548418 1102 88-89 0.1420367232844697 1102 90-91 -1.3558094557603297 1103 1 0.040112189433926915 1103 2 0.13410222236257852 1103 3 0.10342682895307576 1103 4 -0.004724201167441322 1103 5 -0.12360654882259325 1103 6 0.10340047983748235 1103 7 0.030441434586485627 1103 8 0.06039926811144625 1103 9 0.017976475902074185 1103 10-11 0.046969519387666026 1103 12-13 0.04782750701938454 1103 14-15 0.04694450905337533 1103 16-17 -0.05854367777853042 1103 18-19 0.06011772038883123 1103 20-21 -0.024597538354349524 1103 22-23 -0.010594768311271707 1103 24-25 0.04636917079137248 1103 26-27 -0.008143363728635222 1103 28-29 -0.007770337649056103 1103 30-31 0.008191013943523728 1103 32-33 0.03769342027243994 1103 34-35 0.02280305719548892 1103 36-37 -0.06163090651202907 1103 38-39 0.025545447405235677 1103 40-41 0.03758987743717768 1103 42-43 0.021321251217997883 1103 44-45 -0.022602553580981066 1103 46-47 -0.012855474363377084 1103 48-49 0.07449547496182873 1103 50-51 0.04155601179923707 1103 52-53 -0.04686918797296613 1103 54-55 0.02546393615833864 1103 56-57 -0.008150654155691939 1103 58-59 0.021734772467134178 1103 60-61 0.02769067082186183 1103 62-63 -0.03004155720441304 1103 64-65 0.022828768142971967 1103 66-67 0.027204020418125197 1103 68-69 -0.0205792670541598 1103 70-71 0.020044271290707627 1103 72-73 0.030299907844799634 1103 74-75 -0.1418528174290543 1103 76-77 -0.021404231543485253 1103 78-79 -0.01651643998071961 1103 80-81 -0.3118083980544242 1103 82-83 -0.33165652229847353 1103 84-85 -0.03197343243169115 1103 86-87 0.16716457811911312 1103 88-89 0.15220863974921883 1103 90-91 0.5256008006499258 1104 1 0.13292352101075977 1104 2 0.15557283675954636 1104 3 0.10846935610573638 1104 4 0.09388419629657108 1104 5 0.006864240406514455 1104 6 0.05424810902156452 1104 7 0.07536102713557113 1104 8 0.06081027701561226 1104 9 0.13998477582815383 1104 10-11 0.04117245350047938 1104 12-13 0.0331247370034049 1104 14-15 -0.02343769855519895 1104 16-17 -0.021049850580169505 1104 18-19 0.03869771529721788 1104 20-21 -0.07243560084610579 1104 22-23 -0.008598001172352099 1104 24-25 0.012431797506344822 1104 26-27 0.014701369989737145 1104 28-29 0.049970278925641765 1104 30-31 -0.06682271191353806 1104 32-33 0.06299502976873583 1104 34-35 -0.06278237763125816 1104 36-37 -0.05631344988939446 1104 38-39 -0.043478830878285635 1104 40-41 -0.0514639128384502 1104 42-43 0.01646382054450868 1104 44-45 -0.08711807492259993 1104 46-47 -0.08752711087729637 1104 48-49 0.043541403043811044 1104 50-51 0.05433940473482579 1104 52-53 -0.10131576386891794 1104 54-55 -0.08333284788313478 1104 56-57 -0.07831084769460972 1104 58-59 0.1021957162499092 1104 60-61 -0.04553026216256484 1104 62-63 -0.12162889277856692 1104 64-65 0.09068389349742034 1104 66-67 0.1548857825066321 1104 68-69 0.09297287598337078 1104 70-71 -0.002518746307956121 1104 72-73 0.04358807040431856 1104 74-75 -0.06490294176226286 1104 76-77 0.2489208518012589 1104 78-79 -0.019017074021888902 1104 80-81 0.026990128625975274 1104 82-83 -0.2995050736494136 1104 84-85 0.048592096648697236 1104 86-87 0.10896294097010184 1104 88-89 0.16555508652444928 1104 90-91 0.02560080064992576 1201 1 0.09450032293207045 1201 2 -0.045131399392680294 1201 3 -0.04737663210996601 1201 4 -0.0022520132811649773 1201 5 0.10630692460138391 1201 6 -0.005375787158811818 1201 7 0.15157864101417573 1201 8 -0.10770950815555125 1201 9 0.027865227447193774 1201 10-11 -0.035848774802687444 1201 12-13 0.07502157376845275 1201 14-15 0.04323622722395726 1201 16-17 0.07001009230799582 1201 18-19 0.019326620265218253 1201 20-21 0.019901843598681523 1201 22-23 0.015363204494661886 1201 24-25 -0.006782868763629324 1201 26-27 0.06231399103032231 1201 28-29 -0.03496440439813142 1201 30-31 -0.033836180123230974 1201 32-33 -0.0440289886177041 1201 34-35 0.07268855268519303 1201 36-37 -0.0020331291321298295 1201 38-39 0.05532009211881217 1201 40-41 0.08244986715136093 1201 42-43 0.02283024257844346 1201 44-45 0.04326583764019176 1201 46-47 0.02048624537142274 1201 48-49 0.12425632419286359 1201 50-51 0.10228737178378822 1201 52-53 -0.019666276721423515 1201 54-55 0.12830762467007872 1201 56-57 0.14502989922203824 1201 58-59 0.004942262080462001 1201 60-61 0.047008430253264066 1201 62-63 -0.09670613152016472 1201 64-65 0.052819699187274693 1201 66-67 -0.0772618219582526 1201 68-69 -0.14744553944711924 1201 70-71 -0.067553216560853 1201 72-73 -0.009924552982688795 1201 74-75 -0.07353426908239413 1201 76-77 0.14451197743950672 1201 78-79 0.17976376979417807 1201 80-81 0.03374206953725434 1201 82-83 -0.10823267033782713 1201 84-85 0.030672585373700656 1201 86-87 -0.40290835969575767 1201 88-89 -0.05948736010877553 1201 90-91 -0.07838780333867845 1202 1 0.1235002927834401 1202 2 0.13558252023106832 1202 3 -0.0415760351670329 1202 4 -0.09832666129587864 1202 5 -0.0060268810605279555 1202 6 0.0886789024610124 1202 7 -0.14445982864122442 1202 8 -0.061407237963507555 1202 9 0.11564900145304335 1202 10-11 -0.013844795183167946 1202 12-13 -0.09877624950026132 1202 14-15 -0.07825522563865661 1202 16-17 -0.01643809726660095 1202 18-19 -0.04276448890298212 1202 20-21 0.0018789909350473977 1202 22-23 -0.12266929573145546 1202 24-25 0.05364102101296453 1202 26-27 -0.15104637557705303 1202 28-29 0.0036409198504117057 1202 30-31 0.011065686077138537 1202 32-33 -0.0070769873133613714 1202 34-35 0.006335012691806696 1202 36-37 -0.13797307870550668 1202 38-39 -0.029009304670630343 1202 40-41 -0.1227930891840856 1202 42-43 -0.15033092365960954 1202 44-45 0.024661061902776282 1202 46-47 -0.028145961621774518 1202 48-49 0.028201177702364078 1202 50-51 -0.029858362612039002 1202 52-53 -0.08512010652976443 1202 54-55 0.005719055177728194 1202 56-57 -0.12268534440606516 1202 58-59 -0.03832401829616572 1202 60-61 -0.16710892513616926 1202 62-63 -0.10935061576332572 1202 64-65 -0.2204822954910881 1202 66-67 -0.29063297564694324 1202 68-69 -0.2082966622690634 1202 70-71 -0.21744836292764802 1202 72-73 -0.234259283533369 1202 74-75 -0.1419197982218705 1202 76-77 -0.13724575419293217 1202 78-79 -0.3540665150985376 1202 80-81 -0.3786392604065796 1202 82-83 -0.3254036083023948 1202 84-85 -0.2814303670754228 1202 86-87 -0.1198251557396901 1202 88-89 -0.4071409955484313 1202 90-91 0.5256008006499258 1203 1 -0.006853335936220617 1203 2 -0.07117399476684483 1203 3 -0.004388367446836128 1203 4 -0.11528056433123623 1203 5 -0.25010822162943924 1203 6 -0.005584321344819898 1203 7 0.07614366590227917 1203 8 0.1031941097614606 1203 9 -0.0028255647107116033 1203 10-11 -0.01413401934680536 1203 12-13 -0.04563056400917276 1203 14-15 -0.024957473834732014 1203 16-17 -0.016563328347771744 1203 18-19 0.014633089962785562 1203 20-21 -0.0971251238004811 1203 22-23 0.05440745303114625 1203 24-25 -0.022016442163135252 1203 26-27 0.014792374497488936 1203 28-29 0.008590529248706957 1203 30-31 -0.036572813535237 1203 32-33 -0.14696587286061202 1203 34-35 0.017705917067544874 1203 36-37 -0.10280364945284504 1203 38-39 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2401 30-31 0.05607003084971751 2401 32-33 0.022766633871775355 2401 34-35 0.017894057474663327 2401 36-37 0.03071629747410043 2401 38-39 0.03489690989171379 2401 40-41 0.09903423290688806 2401 42-43 0.03967715535649319 2401 44-45 0.1409998680790565 2401 46-47 0.018918183552045775 2401 48-49 0.07331173784816514 2401 50-51 0.03282770438405436 2401 52-53 -0.06269509765439096 2401 54-55 0.11803408848921038 2401 56-57 0.07052905309572566 2401 58-59 0.13370750852197943 2401 60-61 0.07571445693070444 2401 62-63 0.06518742966551372 2401 64-65 0.026476253468459277 2401 66-67 0.022669973497265516 2401 68-69 0.15467147465682274 2401 70-71 0.1443100196921847 2401 72-73 0.024214310160623143 2401 74-75 0.10613821487731911 2401 76-77 -0.04139729458334784 2401 78-79 -0.03376640385759799 2401 80-81 0.3719344737196124 2401 82-83 0.10923654216730938 2401 84-85 0.20291507587439384 2401 86-87 0.14245339186946637 2401 88-89 0.1011257501995857 2401 90-91 0.13194306418191104 2402 1 0.05018716982169735 2402 2 0.12084272876535351 2402 3 0.14603133243206656 2402 4 0.06369161990388506 2402 5 0.15280826661297908 2402 6 -0.02432922762028511 2402 7 -0.03983900277194863 2402 8 0.02718768188399423 2402 9 0.05008931195784072 2402 10-11 -0.015137799638637262 2402 12-13 0.02245382720728628 2402 14-15 -0.03711104301038404 2402 16-17 -0.04525624985491561 2402 18-19 -0.029673680028793115 2402 20-21 0.08119091964755398 2402 22-23 -0.07154164886176773 2402 24-25 0.03238338470779212 2402 26-27 0.006780550149827036 2402 28-29 0.01597383978977973 2402 30-31 0.05641730061022798 2402 32-33 -0.018894842341616425 2402 34-35 -0.014029607000949795 2402 36-37 -0.06343009979665482 2402 38-39 -0.5694845650282545 2402 40-41 0.03116374581603054 2402 42-43 0.03357335464763622 2402 44-45 -0.015680039213378905 2402 46-47 0.016615556483571936 2402 48-49 0.009196947584030113 2402 50-51 0.04008093061859341 2402 52-53 0.022768750371540136 2402 54-55 0.16650890372718408 2402 56-57 -0.03495307892929134 2402 58-59 -0.0737606125685275 2402 60-61 -0.01613587286552587 2402 62-63 0.05997586744232564 2402 64-65 0.07617100408567268 2402 66-67 0.06903828936479073 2402 68-69 0.12040517830256192 2402 70-71 0.10106017390431532 2402 72-73 0.14484577669420418 2402 74-75 0.30310191367102135 2402 76-77 0.22885418498056254 2402 78-79 0.03396431815598078 2402 80-81 0.049452281630820494 2402 82-83 -0.04225450138011411 2402 84-85 -0.11196641139416386 2402 86-87 0.08113286712327294 2402 88-89 0.16372011683458254 2402 90-91 0.08164475669388338 2403 1 -0.1128194929365236 2403 2 -0.0027245766602845833 2403 3 0.14001267881224067 2403 4 0.05033806080970038 2403 5 0.015423822045853797 2403 6 -0.005405697296104961 2403 7 -0.03206154923730509 2403 8 -0.0180722778572715 2403 9 0.10455251868737747 2403 10-11 0.05256329025645812 2403 12-13 -0.0733792979970076 2403 14-15 0.017101094374055492 2403 16-17 -0.09807107460149211 2403 18-19 -0.015349582366766867 2403 20-21 0.011546561784790299 2403 22-23 -0.016114914226726285 2403 24-25 -0.0487571448951698 2403 26-27 -0.05052372749342737 2403 28-29 0.0390459295542982 2403 30-31 0.009404675283690267 2403 32-33 0.009568777603639944 2403 34-35 0.0683938713561929 2403 36-37 0.004002235307360991 2403 38-39 0.04565103157001715 2403 40-41 -0.006380192431542753 2403 42-43 -0.03134126123958225 2403 44-45 0.013444471658708324 2403 46-47 0.04220892121938391 2403 48-49 -0.03843924321987657 2403 50-51 0.0221880609423053 2403 52-53 -0.04542355438972123 2403 54-55 -0.03687821171389061 2403 56-57 -0.0787242679045832 2403 58-59 -0.10668001100511049 2403 60-61 -0.03602495915934156 2403 62-63 -0.0021572778955558647 2403 64-65 0.06622390460130134 2403 66-67 -0.011103575297894963 2403 68-69 -0.009834892715687715 2403 70-71 -0.05781800651917024 2403 72-73 0.01882315597118378 2403 74-75 0.00870864920720038 2403 76-77 0.19653807723472028 2403 78-79 0.1973937002575994 2403 80-81 0.3417131970642018 2403 82-83 0.28346523115495614 2403 84-85 0.2528155840047148 2403 86-87 -0.05771205926683365 2403 88-89 0.0032293011983597353 2403 90-91 0.3986166736657992 2404 1 -0.030831078116477784 2404 2 -0.020083305392702755 2404 3 -0.06788385686594012 2404 4 0.004555312351449459 2404 5 0.03427589438101819 2404 6 -0.06386945750534778 2404 7 0.13215917892816265 2404 8 -0.025197537935468972 2404 9 0.11933276932520442 2404 10-11 0.11433294521492599 2404 12-13 0.10555792487596705 2404 14-15 0.10986083828060345 2404 16-17 0.0042067022848399915 2404 18-19 -0.04343171626490516 2404 20-21 0.06053892925856985 2404 22-23 -0.03625494193273937 2404 24-25 0.03685380486493983 2404 26-27 0.025272660027098937 2404 28-29 0.09028515870659959 2404 30-31 0.07498214248308699 2404 32-33 0.01930749533693188 2404 34-35 0.07503341797293928 2404 36-37 0.06389336935606593 2404 38-39 0.07857431476072207 2404 40-41 0.048417668896568955 2404 42-43 0.0658860777077308 2404 44-45 0.12431236976664906 2404 46-47 0.0912254451272787 2404 48-49 0.10205479959650177 2404 50-51 -0.05172172991108681 2404 52-53 0.07816578223118853 2404 54-55 0.0729123817722126 2404 56-57 -0.040668907462780624 2404 58-59 -0.015256029050505049 2404 60-61 -0.01712785705776554 2404 62-63 0.11356823728521448 2404 64-65 0.1375096325387304 2404 66-67 0.25784892662455405 2404 68-69 0.044415931270194164 2404 70-71 0.019842735513378784 2404 72-73 0.011056250948044521 2404 74-75 0.1693305544627819 2404 76-77 0.11678312702336768 2404 78-79 0.0364290944846033 2404 80-81 -0.0799303323276348 2404 82-83 0.016323614083390225 2404 84-85 -0.3947929496775302 2404 86-87 -0.2151614292069013 2404 88-89 0.2520821656795462 2404 90-91 0.08174115152711892 >>END_MODULE >>Per sequence quality scores pass #Quality Count 29 2.0 30 17.0 31 407.0 32 2619.0 33 4377.0 34 5741.0 35 7146.0 36 10058.0 37 14795.0 38 35541.0 39 254673.0 40 244890.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.28409729331031 15.081014569180343 21.827058624837576 34.80782951267177 2 25.38447218458802 17.7165747932417 28.106200852888758 28.792752169281528 3 26.877156338644692 18.38174216652363 19.69217565737093 35.04892583746075 4 28.26686381762847 21.504275625316666 18.258695150155273 31.970165406899593 5 28.00232996591219 21.077574767434246 19.57378167943667 31.346313587216894 6 27.68592335239356 24.416733015548044 22.15363298900849 25.743710643049912 7 26.07735073225038 24.63353013962562 22.35250729837695 26.936611829747047 8 24.75623248648034 25.348374710908445 23.231414558150917 26.663978244460303 9 24.671616120882494 24.860667349112305 23.734976717574355 26.73273981243085 10-11 24.715302981735963 23.896454384713216 23.48586682659332 27.902375806957497 12-13 25.331227402605013 23.554886896699102 23.024009678319942 28.089876022375943 14-15 25.538546115057574 23.13921546325306 23.07648561177115 28.245752809918212 16-17 25.762064294651072 23.097251949967774 22.70139901355585 28.4392847418253 18-19 25.853660217900064 23.25950512351232 22.590415430164786 28.296419228422824 20-21 25.726821836881708 23.27940978792485 22.67089576159899 28.32287261359446 22-23 25.636001420038397 23.17842162042925 22.6139391244705 28.571637835061853 24-25 26.00514246914346 23.154380921853082 22.616696480579595 28.223780128423858 26-27 26.025305635691215 23.181351311295163 22.623417536095513 28.169925516918106 28-29 25.98696115229912 23.201169808329283 22.62203885804097 28.189830181330635 30-31 26.20917257275802 23.312294004868487 22.508606018827688 27.969927403545807 32-33 26.134916528607437 23.225830053882294 22.654517454693053 27.984735962817208 34-35 26.11793642270977 23.101479477106114 22.78871422470544 27.99186987547867 36-37 26.31488120622484 23.293084879543763 22.630810486523213 27.761223427708188 38-39 26.450680603875874 23.141271415931577 22.73779993398748 27.670248046205064 40-41 26.335208308047093 23.184321519601895 22.80009709916252 27.68037307318849 42-43 26.61319231524341 23.27218490456567 22.5229123465388 27.591710433652118 44-45 26.49410638401008 23.32956987509897 22.710417036953253 27.46590670393769 46-47 26.519449328636124 23.143349337647724 22.860427149632308 27.476774184083848 48-49 26.696799027331757 23.437706160413928 22.589020407495653 27.27647440475866 50-51 26.818577797516358 23.27611043734235 22.64534900272734 27.259962762413952 52-53 26.76027564191401 23.292888648886297 22.696134418442394 27.250701290757302 54-55 27.110769284993193 23.335213097255124 22.496746078300557 27.05727153945113 56-57 26.97669773635153 23.32223701731025 22.597336884154462 27.103728362183755 58-59 26.941596802150762 23.36495808129046 22.581060525664153 27.112384590894617 60-61 27.536989106372182 23.243715853595926 22.348698774731133 26.87059626530076 62-63 27.388679514308585 23.237877967925183 22.38221464657103 26.991227871195207 64-65 27.386983018590687 23.288040246010034 22.4732544552585 26.851722280140777 66-67 27.632449575613766 23.181147516047577 22.156878403455934 27.029524504882723 68-69 27.563875642921097 23.2253295296004 22.195406367782105 27.015388459696393 70-71 27.718270822254908 23.36908354684475 22.076735197500938 26.835910433399402 72-73 28.109970052568666 23.244090844826033 21.79607470148966 26.849864401115635 74-75 28.132282057011526 23.178669576345705 21.790381227927114 26.89866713871566 76-77 28.151818366348657 23.0905406349724 21.802094390666753 26.95554660801219 78-79 28.79403425641822 23.060939138540412 21.121684079698603 27.02334252534276 80-81 28.78288176711522 23.09041065390128 20.837450077232436 27.289257501751063 82-83 29.683593906422512 22.70790071335816 20.489454083646535 27.119051296572792 84-85 30.651986087418866 22.23059143239032 19.41262913814366 27.704793342047157 86-87 31.596304717425593 21.125996026137365 18.83897662795223 28.438722628484815 88-89 31.83340685914089 20.580690489028317 17.745862130941294 29.8400405208895 90-91 29.316215898861014 20.701397314963405 17.245293357861364 32.73709342831422 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 1.0 7 2.0 8 3.0 9 1.5 10 2.0 11 5.0 12 4.0 13 3.5 14 8.0 15 17.0 16 25.0 17 33.5 18 51.0 19 79.0 20 118.0 21 174.0 22 238.5 23 320.0 24 449.0 25 607.0 26 801.0 27 1046.0 28 1357.5 29 1719.5 30 2146.5 31 2651.0 32 3236.0 33 3880.5 34 4710.5 35 5743.5 36 6864.5 37 8154.5 38 9701.0 39 11456.0 40 13337.5 41 15381.0 42 17497.0 43 19542.5 44 21557.5 45 23863.0 46 26157.5 47 27947.0 48 29546.0 49 31283.0 50 32828.0 51 34024.5 52 35101.5 53 35998.5 54 36429.0 55 36623.0 56 36727.5 57 36481.0 58 36130.5 59 35632.0 60 34901.0 61 34051.0 62 32962.5 63 31317.5 64 29256.5 65 26992.0 66 24347.0 67 21888.5 68 19540.5 69 16940.0 70 14445.5 71 12165.0 72 9993.0 73 7936.0 74 6180.0 75 4825.0 76 3673.0 77 2663.0 78 1956.5 79 1447.0 80 1044.5 81 723.0 82 499.0 83 348.0 84 239.5 85 173.0 86 120.0 87 86.5 88 63.0 89 40.0 90 24.5 91 19.0 92 17.0 93 11.0 94 6.0 95 3.0 96 0.5 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.02068017081821096 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 8.71035373617558E-5 34-35 0.0 36-37 0.0 38-39 0.07317039370660705 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 3.240524705760357E-4 64-65 5.247626760797429E-4 66-67 1.9013684148481663E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 2.9019321063964386E-4 76-77 6.6551532515415E-4 78-79 3.8839325593950386E-4 80-81 4.6385631586759685E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 4063.0 32-33 9325.0 34-35 10575.0 36-37 11881.0 38-39 13405.0 40-41 15085.0 42-43 16407.0 44-45 17831.0 46-47 18335.0 48-49 19365.0 50-51 20325.0 52-53 21063.0 54-55 21675.0 56-57 21988.0 58-59 22135.0 60-61 22529.0 62-63 22783.0 64-65 22796.0 66-67 22829.0 68-69 22985.0 70-71 22741.0 72-73 22379.0 74-75 22149.0 76-77 21475.0 78-79 21226.0 80-81 20496.0 82-83 19919.0 84-85 19074.0 86-87 18370.0 88-89 17891.0 90-91 17166.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.71185338200046 #Duplication Level Percentage of deduplicated Percentage of total 1 99.73464388233837 99.44726187901752 2 0.2506550389132113 0.4998655697914749 3 0.010373617761281198 0.0310311795976136 4 0.0022789112767472477 0.00908937868390436 5 0.0011301330559112172 0.005634383078658571 6 2.5468144095303584E-4 0.0015236855099655432 7 4.709465915848824E-4 0.003287127024360525 8 0.0 0.0 9 0.0 0.0 >10 1.92788621936792E-4 0.0023067972965135184 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.002068017081821096 0.0 0.0 0.0 0.0 2 0.002068017081821096 0.0 0.0 0.0 0.0 3 0.002068017081821096 0.0 0.0 0.0 0.0 4 0.002068017081821096 0.0 0.0 0.0 0.0 5 0.002068017081821096 0.0 0.0 0.0 0.0 6 0.0022403518386395205 0.0 0.0 0.0 0.0 7 0.0024126865954579454 0.0 0.0 0.0 0.0 8 0.005342377461371164 0.0 0.0 0.0 0.0 9 0.005859381731826438 0.0 0.0 0.0 0.0 10-11 0.005859381731826438 0.0 0.0 0.0 0.0 12-13 0.005859381731826438 0.0 0.0 0.0 0.0 14-15 0.00594554911023565 8.616737840921232E-5 0.0 0.0 3.446695136368493E-4 16-17 0.006031716488644863 1.7233475681842464E-4 0.0 0.0 4.3083689204606167E-4 18-19 0.006031716488644863 1.7233475681842464E-4 0.0 1.7233475681842464E-4 5.17004270455274E-4 20-21 0.006031716488644863 1.7233475681842464E-4 0.0 1.7233475681842464E-4 6.893390272736986E-4 22-23 0.006031716488644863 1.7233475681842464E-4 0.0 1.7233475681842464E-4 6.893390272736986E-4 24-25 0.006117883867054075 1.7233475681842464E-4 0.0 1.7233475681842464E-4 6.893390272736986E-4 26-27 0.006204051245463287 1.7233475681842464E-4 0.0 1.7233475681842464E-4 8.616737840921232E-4 28-29 0.006204051245463287 1.7233475681842464E-4 0.0 1.7233475681842464E-4 8.616737840921232E-4 30-31 0.0062902186238725 1.7233475681842464E-4 0.0 1.7233475681842464E-4 8.616737840921232E-4 32-33 0.0063763860022817125 1.7233475681842464E-4 0.0 1.7233475681842464E-4 8.616737840921232E-4 34-35 0.0063763860022817125 1.7233475681842464E-4 0.0 1.7233475681842464E-4 8.616737840921232E-4 36-37 0.006548720759100137 1.7233475681842464E-4 0.0 1.7233475681842464E-4 8.616737840921232E-4 38-39 0.006548720759100137 1.7233475681842464E-4 0.0 1.7233475681842464E-4 8.616737840921232E-4 40-41 0.006548720759100137 1.7233475681842464E-4 0.0 1.7233475681842464E-4 8.616737840921232E-4 42-43 0.00663488813750935 1.7233475681842464E-4 0.0 1.7233475681842464E-4 8.616737840921232E-4 44-45 0.0069795576511461985 1.7233475681842464E-4 0.0 1.7233475681842464E-4 8.616737840921232E-4 46-47 0.007238059786373836 1.7233475681842464E-4 0.0 1.7233475681842464E-4 8.616737840921232E-4 48-49 0.007755064056829109 1.7233475681842464E-4 0.0 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