##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-26C_S18_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1216104
Sequences flagged as poor quality	0
Sequence length	61
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.5233302414925	40.0	40.0	40.0	40.0	40.0
2	39.212154552571164	40.0	40.0	40.0	40.0	40.0
3	39.16397939649898	40.0	40.0	40.0	40.0	40.0
4	39.07424036102176	40.0	40.0	40.0	40.0	40.0
5	39.11768072467486	40.0	40.0	40.0	40.0	40.0
6	39.231881483820466	40.0	40.0	40.0	40.0	40.0
7	39.24874188391782	40.0	40.0	40.0	40.0	40.0
8	39.256247820910055	40.0	40.0	40.0	40.0	40.0
9	39.26482603461546	40.0	40.0	40.0	40.0	40.0
10	39.26769421036359	40.0	40.0	40.0	40.0	40.0
11	39.27125311650977	40.0	40.0	40.0	40.0	40.0
12	39.2764697756113	40.0	40.0	40.0	40.0	40.0
13	39.11605915283561	40.0	40.0	40.0	40.0	40.0
14	39.27086828100228	40.0	40.0	40.0	40.0	40.0
15	39.1935623926901	40.0	40.0	40.0	40.0	40.0
16	39.37029069882181	40.0	40.0	40.0	40.0	40.0
17	39.31687750389769	40.0	40.0	40.0	40.0	40.0
18	39.39599244801431	40.0	40.0	40.0	40.0	40.0
19	39.345417826106974	40.0	40.0	40.0	40.0	40.0
20	39.365034569411826	40.0	40.0	40.0	40.0	40.0
21	39.35068382309408	40.0	40.0	40.0	40.0	40.0
22	39.40320564688546	40.0	40.0	40.0	40.0	40.0
23	39.395357633886576	40.0	40.0	40.0	40.0	40.0
24	39.40397860709282	40.0	40.0	40.0	40.0	40.0
25	39.35587416865663	40.0	40.0	40.0	40.0	40.0
26	39.32101859709367	40.0	40.0	40.0	40.0	40.0
27	39.35271325478742	40.0	40.0	40.0	40.0	40.0
28	39.35670962351904	40.0	40.0	40.0	40.0	40.0
29	39.363541276075075	40.0	40.0	40.0	40.0	40.0
30	39.364310947090054	40.0	40.0	40.0	40.0	40.0
31	39.324284765118776	40.0	40.0	40.0	40.0	40.0
32	39.341539868300735	40.0	40.0	40.0	40.0	40.0
33	39.35001282785025	40.0	40.0	40.0	40.0	40.0
34	39.33664883924401	40.0	40.0	40.0	40.0	40.0
35	39.3469144086361	40.0	40.0	40.0	40.0	40.0
36	39.309766269990064	40.0	40.0	40.0	40.0	40.0
37	39.3210383322479	40.0	40.0	40.0	40.0	40.0
38	39.312391045502686	40.0	40.0	40.0	40.0	40.0
39	38.029459651477175	40.0	40.0	40.0	40.0	40.0
40	39.298507364501724	40.0	40.0	40.0	40.0	40.0
41	39.312703518778	40.0	40.0	40.0	40.0	40.0
42	39.28471249169479	40.0	40.0	40.0	40.0	40.0
43	39.3064145829633	40.0	40.0	40.0	40.0	40.0
44	39.25488938446054	40.0	40.0	40.0	40.0	40.0
45	39.123486149210926	40.0	40.0	40.0	40.0	40.0
46	39.125696486484706	40.0	40.0	40.0	40.0	40.0
47	39.214928986336695	40.0	40.0	40.0	40.0	40.0
48	39.28765960805984	40.0	40.0	40.0	40.0	40.0
49	39.2722497417984	40.0	40.0	40.0	40.0	40.0
50	39.2440482064034	40.0	40.0	40.0	40.0	40.0
51	39.21435995605639	40.0	40.0	40.0	40.0	40.0
52	39.23030267148204	40.0	40.0	40.0	40.0	40.0
53	39.15637807292797	40.0	40.0	40.0	40.0	40.0
54	39.08464736568583	40.0	40.0	40.0	40.0	40.0
55	39.12622933564892	40.0	40.0	40.0	40.0	40.0
56	39.13078815627611	40.0	40.0	40.0	40.0	40.0
57	39.065714774394294	40.0	40.0	40.0	40.0	40.0
58	39.04052285001941	40.0	40.0	40.0	40.0	40.0
59	38.985534131949244	40.0	40.0	40.0	40.0	40.0
60	38.96252294211679	40.0	40.0	40.0	40.0	40.0
61	38.828320604158854	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.06381876362631544
1101	2	0.014532790815984242
1101	3	0.17276810510444562
1101	4	-0.09168029233002528
1101	5	0.09355549615548142
1101	6	0.08993313814985271
1101	7	-0.04069263039956894
1101	8	0.059673409213218065
1101	9	0.09144868947490181
1101	10	0.019393645005791882
1101	11	0.015219919311128649
1101	12	0.02186322989931
1101	13	0.06550289469014103
1101	14	0.048480770321383204
1101	15	0.05626098540248847
1101	16	0.1114038002705442
1101	17	0.11086510314618181
1101	18	0.004028770176873309
1101	19	0.10646489363250566
1101	20	0.09853807150810212
1101	21	0.055284124059134854
1101	22	0.05698905280707578
1101	23	0.0734757844240832
1101	24	0.12595553896041878
1101	25	0.0781840602224193
1101	26	-1.7413739931271266E-4
1101	27	0.007417436511978792
1101	28	0.029576174574202696
1101	29	0.19045836124697502
1101	30	0.071290428712949
1101	31	0.014937872104880512
1101	32	0.07210844395022775
1101	33	0.08907768849603315
1101	34	0.12151818542213277
1101	35	0.14605964539081384
1101	36	-0.049182022564380645
1101	37	0.06945769568743998
1101	38	-0.024429341494254686
1101	39	1.229317358409574
1101	40	0.036901517645020476
1101	41	-0.037733956118017886
1101	42	0.06892851679757683
1101	43	-0.001752203740721825
1101	44	0.07250270574984086
1101	45	0.10959939790502204
1101	46	0.15312956391425558
1101	47	0.07620603030462547
1101	48	0.0032618566147206707
1101	49	-0.04945073514119258
1101	50	0.06976399022413204
1101	51	0.08056571546357105
1101	52	-0.05088870743325202
1101	53	-0.06109286120902624
1101	54	0.08044968451140022
1101	55	-0.013961942781456571
1101	56	0.18011580081645917
1101	57	0.07933366414037835
1101	58	0.057164193904824856
1101	59	0.1207313413316271
1101	60	0.13474227104310899
1101	61	0.021554052102182197
1102	1	0.11975215177662335
1102	2	3.7291161945773865E-4
1102	3	0.04261611386004205
1102	4	0.015788758811204673
1102	5	-0.06228546052302164
1102	6	0.05108265640200216
1102	7	0.0781883282371183
1102	8	-0.024331770607972203
1102	9	-0.042348602237929356
1102	10	0.08443435680676004
1102	11	0.02948005143146304
1102	12	-0.0819805713582511
1102	13	-0.049379925265768065
1102	14	-0.031977799571961896
1102	15	0.09602728411922357
1102	16	-0.00948794558073729
1102	17	0.01046557571503115
1102	18	-0.002989976078289658
1102	19	-0.018296615077652234
1102	20	-0.026778343645240454
1102	21	-0.006293565316688898
1102	22	0.09426230868612606
1102	23	0.010940354129417074
1102	24	0.021086033113590474
1102	25	-0.002300809788593483
1102	26	0.05586151826913266
1102	27	-0.07859462456574562
1102	28	-0.0302073780613199
1102	29	0.061416964080564185
1102	30	0.0494850271895686
1102	31	0.03432600637713534
1102	32	0.02825208633945664
1102	33	0.1104997853341061
1102	34	0.03420257593773357
1102	35	-0.02948608856083723
1102	36	-0.07015545501877796
1102	37	-0.057702592072303105
1102	38	0.04450378772239105
1102	39	1.3505780870999047
1102	40	0.026244762043155845
1102	41	-0.025705167644659355
1102	42	0.09821513001163851
1102	43	-0.10857234504786817
1102	44	0.044599843281488916
1102	45	-0.010969976206865795
1102	46	0.0748869742328111
1102	47	0.015475673432860049
1102	48	0.005483565565121751
1102	49	-0.07717522552432854
1102	50	0.004149102586829656
1102	51	-0.0027705039387768693
1102	52	0.0315384653275359
1102	53	0.10708962098566843
1102	54	-0.0035635676231464686
1102	55	-0.09068459397913387
1102	56	-0.04833587480049317
1102	57	-0.03987513457700942
1102	58	-0.012518618864113762
1102	59	-0.044278461321148654
1102	60	-0.017503121033058733
1102	61	0.021155383981806608
1103	1	0.03998588722367913
1103	2	0.14237368879898327
1103	3	-4.945773908886508E-4
1103	4	-0.047027211039591066
1103	5	0.11720569735032171
1103	6	0.040012263230558176
1103	7	0.08986049444810362
1103	8	0.03983779023248246
1103	9	0.016651181126434267
1103	10	-7.831854084727752E-4
1103	11	0.06999908200589999
1103	12	0.05846226163041024
1103	13	0.04145726359104884
1103	14	-0.06677414381402969
1103	15	0.007659861046953154
1103	16	-0.0410770466636734
1103	17	0.02317637138659734
1103	18	0.0737313062719096
1103	19	-0.03652693892834691
1103	20	0.03648112178191809
1103	21	-0.010059559788793138
1103	22	-0.013498087119067748
1103	23	0.03198249304750789
1103	24	0.021511735753655614
1103	25	0.007355708862746724
1103	26	0.0294273229543478
1103	27	0.014505719561590524
1103	28	0.01035520537211454
1103	29	0.054629622798699984
1103	30	0.09219816581904894
1103	31	-0.012745870512738122
1103	32	-0.0012640048817402771
1103	33	-0.06020166200839583
1103	34	-0.04377855252879215
1103	35	-0.12609987369135212
1103	36	0.09921497446220684
1103	37	0.0848557413722375
1103	38	0.054147559195499184
1103	39	1.2220844058827964
1103	40	0.06678397564981253
1103	41	0.06429947113317525
1103	42	0.06356219152480946
1103	43	0.03599844916175243
1103	44	0.0294514153372063
1103	45	0.03967190728963743
1103	46	-0.0012027916619459234
1103	47	0.09923897506356383
1103	48	0.026415228938432733
1103	49	0.13649828750570947
1103	50	0.19516609968757592
1103	51	0.04178725760891666
1103	52	0.05449427437932286
1103	53	0.13519077542191127
1103	54	0.006813963297560122
1103	55	0.12279346231784416
1103	56	-0.005982843956374495
1103	57	0.027172367317973567
1103	58	0.0627773696188072
1103	59	0.03403887397153227
1103	60	0.03398193519096537
1103	61	0.09316127264702345
1104	1	-0.06979982729317413
1104	2	-0.06732845696821244
1104	3	-0.08802953927663282
1104	4	0.030947906560705007
1104	5	-0.077810703589833
1104	6	-0.10566297981723949
1104	7	-0.017045482483787566
1104	8	-0.034144148778239014
1104	9	0.02817633372906414
1104	10	-0.04154445653797012
1104	11	-0.1299586347416124
1104	12	-0.14148062361909552
1104	13	0.006653670377509968
1104	14	-0.10307652731665229
1104	15	0.04347388376988448
1104	16	0.0194403191328405
1104	17	-0.05013815021399637
1104	18	-0.03839077367580046
1104	19	-0.07374968429795103
1104	20	0.015430372171046258
1104	21	-0.027860593355441665
1104	22	-0.008650344580928504
1104	23	-0.05366967758267549
1104	24	-0.03504454142169777
1104	25	0.08308102304781073
1104	26	0.08268183901065385
1104	27	0.07353912656702022
1104	28	-0.02686052874388878
1104	29	-0.08145422532244595
1104	30	-0.04312927590395077
1104	31	-0.03215652610809627
1104	32	-0.057723472824669386
1104	33	-0.02959592205687045
1104	34	-0.12184077147190209
1104	35	0.0058743011650008725
1104	36	-0.10792442328190077
1104	37	-0.059922600541788995
1104	38	-0.07218794978376053
1104	39	1.0818024348360424
1104	40	-0.1966994740163983
1104	41	-0.010632492740533905
1104	42	-0.060696098743250104
1104	43	-0.10526320974399539
1104	44	-0.058099187037342404
1104	45	-0.10943022078762965
1104	46	-0.05128849075403963
1104	47	0.021369565594490325
1104	48	-0.08902209530831584
1104	49	-0.08622766632141321
1104	50	-0.02956291536072797
1104	51	-0.1927852878320948
1104	52	-0.06353937100872997
1104	53	-0.0017454793390001555
1104	54	-0.010688822327431069
1104	55	-0.06082048295770193
1104	56	0.0011513762466108801
1104	57	-0.023625243678260688
1104	58	-0.13226788532655576
1104	59	-0.08247252039421937
1104	60	0.020344777272725878
1104	61	0.0313109596754515
1201	1	0.03866097248732814
1201	2	0.010283176202783295
1201	3	0.1391679708406528
1201	4	0.041206669164445486
1201	5	0.03190189672568522
1201	6	0.11446528686771984
1201	7	0.05316252218045037
1201	8	0.10584316173688535
1201	9	0.0510131195168384
1201	10	0.02435083806386018
1201	11	-0.013849848130732312
1201	12	2.214821494703756E-4
1201	13	-0.0690716880146951
1201	14	-0.0331000200116236
1201	15	-0.016016650560317203
1201	16	0.0062149607406354335
1201	17	-0.07607442842238044
1201	18	-0.03357648600174912
1201	19	0.07859272601825751
1201	20	0.02899440262580555
1201	21	0.03000088548398594
1201	22	-0.023946318241677034
1201	23	-0.04631320594538835
1201	24	-0.018788282419016866
1201	25	0.09230662151135505
1201	26	0.125873244456848
1201	27	0.09431387106252487
1201	28	0.02562077998784673
1201	29	0.013318221347418557
1201	30	0.1150991691197163
1201	31	0.06547534164689495
1201	32	0.047997941538454825
1201	33	0.01400703425014882
1201	34	0.015008463729415666
1201	35	-0.06880074513447454
1201	36	0.08041153940416024
1201	37	0.06725518943008524
1201	38	0.08180066397575558
1201	39	1.3261779064047374
1201	40	0.050432530922215335
1201	41	0.01709145951956259
1201	42	0.036918621059207624
1201	43	0.09246554394281503
1201	44	0.12618128487430624
1201	45	0.05324383093059737
1201	46	0.15289646706199278
1201	47	0.12003031050154789
1201	48	-9.887939844901439E-4
1201	49	0.09408305457851185
1201	50	0.015416164137178612
1201	51	0.016848431901770766
1201	52	0.044732801293896785
1201	53	0.09062625397604052
1201	54	0.07217008431911864
1201	55	0.08159326284105362
1201	56	-0.08330228998564593
1201	57	0.13138367325705502
1201	58	0.011204746541203292
1201	59	0.04846924515896944
1201	60	-0.11419377132457242
1201	61	0.028485834495398876
1202	1	0.056420535328861376
1202	2	0.06334840844321832
1202	3	-0.02266604555278917
1202	4	-0.06425781170986511
1202	5	-0.03529414152568222
1202	6	-0.05715356559129958
1202	7	0.04973014513127083
1202	8	0.07327827102650275
1202	9	0.014486480172578808
1202	10	0.03872925336440147
1202	11	-0.0023915147974022943
1202	12	-0.08058452877948952
1202	13	-0.0793688191622337
1202	14	0.08573536250203517
1202	15	-0.03883085821058785
1202	16	0.027167829593096826
1202	17	0.06437980655029918
1202	18	0.03404646481792639
1202	19	0.02722660579967595
1202	20	0.033605058363512796
1202	21	0.04682124067524285
1202	22	-0.07071885922528054
1202	23	-0.0873821950164313
1202	24	0.09784423124218478
1202	25	-0.08441810526460358
1202	26	0.0477140914513825
1202	27	0.015471657947770723
1202	28	-0.016814329301766406
1202	29	0.037191172167091224
1202	30	0.04196050791752981
1202	31	0.0739452870020827
1202	32	0.007731548095833318
1202	33	0.06802001239222477
1202	34	-0.052973093647636915
1202	35	-0.0341012915825587
1202	36	-0.4087961108690692
1202	37	-0.1640631165808415
1202	38	-0.23671026498599446
1202	39	1.1750337807216766
1202	40	-0.0897826330122129
1202	41	-0.2004119557809787
1202	42	-0.1189727723834153
1202	43	-0.11755836316100954
1202	44	-0.07667049108197688
1202	45	-0.07510316360492197
1202	46	-0.1089375493314435
1202	47	-0.05148608294107504
1202	48	-0.1565386573724723
1202	49	-0.021439486405093078
1202	50	-0.042917169196151406
1202	51	-0.09703476481953999
1202	52	-0.05902402930719575
1202	53	-0.15858822689827434
1202	54	-0.07238251130929996
1202	55	-0.038113021311957596
1202	56	-0.02852086922061403
1202	57	-0.11544009723474602
1202	58	-0.1099701168467746
1202	59	-0.04980602806507051
1202	60	-0.07638639427539573
1202	61	-0.039888482444496276
1203	1	0.005812104211955216
1203	2	0.022237885844347716
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2403	4	0.09349052969231764
2403	5	0.06927876953761825
2403	6	0.14861902720052456
2403	7	0.03721165400700954
2403	8	0.024267266005878696
2403	9	-0.05351546980052291
2403	10	0.014086748433435048
2403	11	0.13797909215974613
2403	12	0.08668057790869454
2403	13	0.13290760633534404
2403	14	0.07379962196052503
2403	15	0.052304608886416304
2403	16	-0.021465508222938467
2403	17	0.02252542695553217
2403	18	0.02527158585073863
2403	19	-0.03336152401922021
2403	20	0.10987254088099263
2403	21	0.10570426470250283
2403	22	0.004266011673053072
2403	23	-0.040182199499128046
2403	24	0.017620768807184106
2403	25	-0.09828588812276706
2403	26	-0.12184223363379232
2403	27	-0.011389221557337237
2403	28	0.08339355576188723
2403	29	0.03989087630910859
2403	30	-0.07948863588738675
2403	31	0.004494865628586808
2403	32	0.01888337703611853
2403	33	0.04145050817848528
2403	34	0.09050626353364777
2403	35	0.039892342987997154
2403	36	0.055651620886358444
2403	37	0.06160368456672671
2403	38	0.03941713325679075
2403	39	1.2978310955862966
2403	40	-0.011778665825552537
2403	41	-0.05649329431424377
2403	42	0.013284153383125386
2403	43	0.043012396207828374
2403	44	0.002135878830159754
2403	45	-0.004207072516521748
2403	46	0.03883847272341967
2403	47	0.04475517580018362
2403	48	0.032446796276445866
2403	49	0.08033734253519498
2403	50	0.08655656220065566
2403	51	-0.006635020333256136
2403	52	0.032111437789808406
2403	53	0.06417154538073078
2403	54	0.06246541320209076
2403	55	0.019685750429125903
2403	56	-0.028202597748496316
2403	57	0.04462916043066656
2403	58	0.06440902964775574
2403	59	0.11883582687485728
2403	60	0.02742950136742195
2403	61	0.00786120376672983
2404	1	-0.04377895687280642
2404	2	-0.08899392470875256
2404	3	-0.03327200311535705
2404	4	0.13070501019799963
2404	5	-0.06072372984631613
2404	6	-0.06106406613537274
2404	7	0.008289040669900771
2404	8	-0.028606703583584192
2404	9	0.09952116461218452
2404	10	-0.05496180575783427
2404	11	0.03321217580833036
2404	12	0.0706985687903341
2404	13	-0.02666547313394574
2404	14	0.02733742089884572
2404	15	0.12004201084998556
2404	16	0.00924413536023394
2404	17	0.02367092914732183
2404	18	-0.0019301401875111424
2404	19	0.040320548491003194
2404	20	-0.027704789296990384
2404	21	0.03206901905754478
2404	22	0.05736533555165124
2404	23	0.0721378902211498
2404	24	-8.96063304622885E-6
2404	25	0.02828478737232132
2404	26	-0.007074854867084923
2404	27	-0.0808252680587529
2404	28	0.10103908926914329
2404	29	0.08631385987111884
2404	30	0.05714571829112458
2404	31	0.053334115438786966
2404	32	0.07367958944512054
2404	33	0.02907709436538397
2404	34	0.053572744219614776
2404	35	0.05019776536340004
2404	36	0.1475060291381851
2404	37	0.04020556314349477
2404	38	0.05371864324759201
2404	39	1.2881197890640763
2404	40	0.08983855850030409
2404	41	0.04158908701916175
2404	42	0.011933783655756258
2404	43	0.07801313665763132
2404	44	0.10475519807290112
2404	45	0.08580996660907658
2404	46	0.07947716775233715
2404	47	0.06784199105239708
2404	48	0.0769703651157414
2404	49	0.044817994552467155
2404	50	0.10491562006753696
2404	51	0.07569714486489687
2404	52	0.008445140079821556
2404	53	0.07471110208250309
2404	54	0.07863648578899785
2404	55	0.07395398993214997
2404	56	-0.039665229032003424
2404	57	0.016979652493219533
2404	58	0.06495280662075942
2404	59	0.07168585622945045
2404	60	0.008073616605997813
2404	61	0.0519867442511881
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	6.0
17	32.0
18	100.0
19	120.0
20	160.0
21	244.0
22	340.0
23	503.0
24	813.0
25	995.0
26	1360.0
27	1885.0
28	2466.0
29	2964.0
30	3786.0
31	4911.0
32	6315.0
33	8014.0
34	10465.0
35	13645.0
36	19842.0
37	29325.0
38	78350.0
39	541040.0
40	488422.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	26.62247636715281	17.288981863393264	23.184283581009517	32.90425818844441
2	26.13582802980014	18.002071236383347	26.725118635451707	29.13698209836481
3	26.98938577621651	20.458858781814712	20.339625558340405	32.21212988362838
4	27.713665936465958	21.82231124969575	19.87519159545565	30.58883121838264
5	27.23056580687178	22.003463519567408	19.91120825192582	30.85476242163499
6	28.42117121561972	24.67058738397374	22.1128291659266	24.795412234479947
7	26.70684415148704	25.039305848841874	22.035944294237993	26.217905705433086
8	24.763753757902286	27.066435107523702	22.628410070191364	25.54140106438265
9	24.885207186227493	26.447080183931636	23.30294119581878	25.36477143402209
10	25.769917704406858	24.330484892739438	23.4688809509713	26.430716451882404
11	24.971055107128993	25.268973706196178	23.287399761862474	26.47257142481235
12	26.737351410734604	24.258698269226976	22.795912191720443	26.208038128317973
13	25.522817127482515	23.506624433436617	23.113237025780688	27.85732141330018
14	25.30836178484735	24.49313545552025	23.14736239663713	27.05114036299527
15	25.855683395499067	23.74040378125555	22.611799648714253	27.792113174531124
16	25.662607803279986	24.68900686125529	22.80569753902627	26.842687796438465
17	25.58202259017321	23.509502476761856	22.918927986422215	27.989546946642722
18	27.02893831448626	23.783163282087717	22.71647819594377	26.471420207482254
19	25.57347069000678	23.79212633130061	23.864077414431662	26.77032556426095
20	25.374063402472157	23.799033635281194	22.925259681737746	27.901643280508903
21	25.78751488359548	23.96554899909876	23.67091959240328	26.576016524902474
22	26.866287751705446	23.52068573082565	22.749616809088696	26.863409708380203
23	25.519116786064348	24.615986790603436	23.022784235558802	26.842112187773417
24	26.02277436798169	24.848286001855104	22.6748699124417	26.454069717721513
25	25.619848302447817	23.480968732937317	22.82263687974055	28.07654608487432
26	25.679793833422142	23.63144928394282	23.9996743699552	26.689082512679835
27	26.108046680218138	23.885539394657034	22.628656759619243	27.377757165505585
28	25.73891706630354	24.04917671514936	22.693124930104663	27.518781288442433
29	25.832659048897135	24.978784709202504	22.842700953207952	26.345855288692416
30	27.23064803668107	24.350302276778958	22.40786972166854	26.011179964871427
31	25.867442258227914	24.048436646865728	23.713925782663324	26.370195312243034
32	25.888328629788244	24.081904179247825	22.788100359837646	27.241666831126288
33	26.10245505318624	25.390509364330683	22.494128791616507	26.01290679086657
34	26.191345477031568	24.18625380724017	22.56566872570109	27.056731990027167
35	26.21165623992685	24.10920447593298	23.645675040950444	26.033464243189723
36	26.358518679323478	25.82756080072099	22.121216606474448	25.692703913481086
37	27.31411129311309	24.53194792550637	22.205008782143633	25.94893199923691
38	26.25277114457316	24.637202081400932	22.26413201502503	26.845894759000878
39	26.798613522317183	24.78528357339749	21.81121593549466	26.60488696879067
40	26.345115220408783	25.926401031490727	21.945409274206813	25.78307447389368
41	26.291830303987158	24.67905705433088	23.26544440278134	25.76366823890062
42	27.90090321222527	24.977469032253822	21.661634202337957	25.45999355318295
43	26.586459710682643	26.087160308657815	21.763105787005056	25.56327419365449
44	26.38483221829712	25.16051258773921	22.800928210087296	25.65372698387638
45	26.884953918414872	25.33319518725372	21.37555669580891	26.406294198522495
46	26.555623532197902	25.554228914632297	22.582361376987496	25.3077861761823
47	27.549370777499295	25.6434482577148	21.39307164518824	25.414109319597667
48	27.95065224684731	25.52355719576615	21.293984724990626	25.231805832395914
49	27.67740259056791	25.86415306585621	21.093179530698034	25.365264812877847
50	27.921954043404185	25.682014038273042	21.10321156743173	25.29282035089104
51	28.02498799444784	25.927963397867288	20.797727825909625	25.249320781775243
52	27.696891055370266	26.26987494490603	20.74904777880839	25.284186220915316
53	27.586949800346023	26.387628031813072	20.761957858867333	25.263464308973578
54	28.097679145862525	26.302849098432368	20.29530369113168	25.304168064573425
55	27.89654503233276	26.307289508134176	20.526616144671834	25.26954931486123
56	27.76604632498536	26.47898534993718	20.454500601922206	25.300467723155258
57	27.9607665133903	26.674692296053625	19.993602520837033	25.370938669719038
58	27.685872260925056	26.531859117312333	20.269812450250964	25.512456171511648
59	27.4678810364903	26.60964851690316	20.164887213593573	25.757583233012966
60	27.784054653220448	26.878046614434293	19.809572207640137	25.528326524705125
61	27.83273470032168	26.934209574181157	19.797895574720584	25.435160150776575
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	6.0
2	4.5
3	7.0
4	5.0
5	3.0
6	5.0
7	8.0
8	9.0
9	10.0
10	11.0
11	12.0
12	16.5
13	20.0
14	31.0
15	42.0
16	47.5
17	77.0
18	101.0
19	165.5
20	304.0
21	378.0
22	536.0
23	694.0
24	907.0
25	1553.5
26	1987.0
27	2418.5
28	2850.0
29	3787.5
30	5930.5
31	7136.0
32	8421.0
33	9706.0
34	11348.0
35	15195.5
36	17401.0
37	20309.0
38	23217.0
39	26566.5
40	33734.0
41	37552.0
42	40733.5
43	48108.5
44	52302.0
45	55729.0
46	59156.0
47	62376.0
48	68151.0
49	70706.0
50	72428.0
51	74150.0
52	76282.5
53	80164.5
54	81914.0
55	81334.0
56	80754.0
57	79111.0
58	74847.5
59	72227.0
60	68682.5
61	65641.0
62	66144.0
63	55804.0
64	45464.0
65	41016.0
66	32793.5
67	29019.0
68	25298.0
69	21577.0
70	18702.0
71	13434.0
72	11041.0
73	9218.0
74	7395.0
75	6031.0
76	3785.5
77	2904.0
78	2318.0
79	1732.0
80	1334.5
81	696.5
82	456.0
83	343.0
84	170.0
85	110.0
86	81.0
87	52.0
88	38.0
89	20.5
90	17.0
91	10.0
92	3.0
93	3.0
94	3.5
95	4.0
96	3.0
97	2.0
98	2.5
99	3.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.033303072763513644
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.1374783735601564
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1216104.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.94150398430777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.99797061260674	63.78932219951041
2	12.041215234161267	18.288559893619304
3	2.3359092493732376	5.321773847047772
4	0.6756027421380487	2.0522515333554354
5	0.26637643466873506	1.011451353736072
6	0.1325019590630064	0.6037438831267131
7	0.0891007035196944	0.47365090019418477
8	0.06169220616997936	0.37479991365265786
9	0.051403555669148296	0.35132969948905907
>10	0.30988664224353973	4.6476681891971445
>50	0.03589041441242828	1.8000222482423567
>100	0.0023335675944867774	0.25439160534760685
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	1.1667837972433887E-4	1.0310347334812795
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCCATGATCTGGGGGGGGGGGGGGG	11636	0.9568260609289996	TruSeq Adapter, Index 10 (97% over 37bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0706321169899944	0.0	0.0	0.0	0.0
2	1.0727700920316026	0.0	0.0	0.0	0.0
3	1.073756849743114	0.0	0.0	0.0	0.0
4	1.0750725266917962	0.0	0.0	0.0	0.0
5	1.0760592844033077	0.0	0.0	0.0	0.0
6	1.07926624696572	0.0	0.0	0.0	0.0
7	1.082802128765303	0.0	0.0	0.0	0.0
8	1.1152829034358904	0.0	0.0	0.0	0.0
9	1.119558853519107	0.0	0.0	0.0	0.0
10	1.1234236545558605	0.0	0.0	0.0	0.0
11	1.1276173748297842	0.0	0.0	0.0	0.0
12	1.1351825172847059	0.0	0.0	0.0	0.0
13	1.1500661127666714	0.0	0.0	0.0	0.0
14	1.1704591054712425	0.0	0.0	0.0	0.0
15	1.1976771723471018	0.0	0.0	0.0	0.0
16	1.227773282548203	0.0	0.0	0.0	0.0
17	1.256635945609915	0.0	0.0	0.0	0.0
18	1.2924881424615	0.0	0.0	0.0	0.0
19	1.3447040713623177	0.0	0.0	0.0	8.222980929262628E-5
20	1.4109812976521745	0.0	0.0	0.0	1.6445961858525257E-4
21	1.6041391196805537	0.0	8.222980929262628E-5	0.0	3.2891923717050513E-4
22	1.6996079282692929	0.0	8.222980929262628E-5	0.0	3.2891923717050513E-4
23	1.8173610151763335	0.0	8.222980929262628E-5	0.0	3.2891923717050513E-4
24	1.9590429765875288	0.0	8.222980929262628E-5	0.0	3.2891923717050513E-4
25	2.121611309559051	0.0	8.222980929262628E-5	0.0	3.2891923717050513E-4
26	2.3161670383454047	0.0	8.222980929262628E-5	0.0	3.2891923717050513E-4
27	2.5228105490977746	0.0	8.222980929262628E-5	0.0	3.2891923717050513E-4
28	2.7603724681441717	0.0	8.222980929262628E-5	0.0	3.2891923717050513E-4
29	3.0379803043160782	0.0	8.222980929262628E-5	0.0	3.2891923717050513E-4
30	3.3505358094373507	0.0	8.222980929262628E-5	0.0	3.2891923717050513E-4
31	3.6863623505884364	0.0	8.222980929262628E-5	0.0	3.2891923717050513E-4
32	4.066017380092492	0.0	8.222980929262628E-5	0.0	3.2891923717050513E-4
33	4.478399873695013	0.0	1.6445961858525257E-4	0.0	4.1114904646313144E-4
34	4.956895133968805	0.0	1.6445961858525257E-4	0.0	4.1114904646313144E-4
35	5.461950622644116	0.0	1.6445961858525257E-4	0.0	4.1114904646313144E-4
36	5.97276219796991	0.0	1.6445961858525257E-4	0.0	4.1114904646313144E-4
37	6.568106017248525	0.0	1.6445961858525257E-4	0.0	4.1114904646313144E-4
38	7.177675593534763	0.0	1.6445961858525257E-4	0.0	4.1114904646313144E-4
39	7.842750291093525	0.0	1.6445961858525257E-4	0.0	4.1114904646313144E-4
40	8.579611612164749	0.0	1.6445961858525257E-4	0.0	4.933788557557578E-4
41	9.322229019886457	0.0	1.6445961858525257E-4	0.0	4.933788557557578E-4
42	10.136468591502043	0.0	1.6445961858525257E-4	0.0	4.933788557557578E-4
43	10.956135330530941	0.0	1.6445961858525257E-4	0.0	4.933788557557578E-4
44	11.841339227566063	0.0	1.6445961858525257E-4	0.0	5.75608665048384E-4
45	12.793642648983969	0.0	1.6445961858525257E-4	0.0	5.75608665048384E-4
46	13.74553492135541	0.0	1.6445961858525257E-4	0.0	5.75608665048384E-4
47	14.693233473452928	0.0	1.6445961858525257E-4	0.0	5.75608665048384E-4
48	15.701288705571233	0.0	1.6445961858525257E-4	0.0	5.75608665048384E-4
49	16.74947208462434	0.0	1.6445961858525257E-4	0.0	5.75608665048384E-4
50	17.804973916704494	0.0	1.6445961858525257E-4	0.0	5.75608665048384E-4
>>END_MODULE