##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-26C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 547545 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.60634468399858 40.0 40.0 40.0 40.0 40.0 2 39.336343131614754 40.0 40.0 40.0 40.0 40.0 3 39.303896483394055 40.0 40.0 40.0 40.0 40.0 4 39.20857646403492 40.0 40.0 40.0 40.0 40.0 5 39.25738341140911 40.0 40.0 40.0 40.0 40.0 6 39.373603996018595 40.0 40.0 40.0 40.0 40.0 7 39.39827411445635 40.0 40.0 40.0 40.0 40.0 8 39.40077253924335 40.0 40.0 40.0 40.0 40.0 9 39.40948049931969 40.0 40.0 40.0 40.0 40.0 10-11 39.41201910345268 40.0 40.0 40.0 40.0 40.0 12-13 39.33767270270023 40.0 40.0 40.0 40.0 40.0 14-15 39.36866558912966 40.0 40.0 40.0 40.0 40.0 16-17 39.479220885954575 40.0 40.0 40.0 40.0 40.0 18-19 39.50830707978339 40.0 40.0 40.0 40.0 40.0 20-21 39.4942954460364 40.0 40.0 40.0 40.0 40.0 22-23 39.53167684847821 40.0 40.0 40.0 40.0 40.0 24-25 39.50989233761609 40.0 40.0 40.0 40.0 40.0 26-27 39.48286990110402 40.0 40.0 40.0 40.0 40.0 28-29 39.50698116136573 40.0 40.0 40.0 40.0 40.0 30-31 39.498728838763185 40.0 40.0 40.0 40.0 40.0 32-33 39.502370190709634 40.0 40.0 40.0 40.0 40.0 34-35 39.49596316813408 40.0 40.0 40.0 40.0 40.0 36-37 39.47746154106699 40.0 40.0 40.0 40.0 40.0 38-39 39.423095044736485 40.0 40.0 40.0 40.0 40.0 40-41 39.47329246957652 40.0 40.0 40.0 40.0 40.0 42-43 39.45748234187624 40.0 40.0 40.0 40.0 40.0 44-45 39.369986819944295 40.0 40.0 40.0 40.0 40.0 46-47 39.34443159694305 40.0 40.0 40.0 40.0 40.0 48-49 39.432940298973044 40.0 40.0 40.0 40.0 40.0 50-51 39.39349880233229 40.0 40.0 40.0 40.0 40.0 52-53 39.355682668309555 40.0 40.0 40.0 40.0 40.0 54-55 39.27692467608001 40.0 40.0 40.0 40.0 40.0 56-57 39.291851526993696 40.0 40.0 40.0 40.0 40.0 58-59 39.23692092332665 40.0 40.0 40.0 40.0 40.0 60-61 39.16710292219716 40.0 40.0 40.0 40.0 40.0 62-63 39.433250522610805 40.0 40.0 40.0 40.0 40.0 64-65 39.42950329388228 40.0 40.0 40.0 40.0 40.0 66-67 39.42165842516749 40.0 40.0 40.0 40.0 40.0 68-69 39.43581946891191 40.0 40.0 40.0 40.0 40.0 70-71 39.41374069147554 40.0 40.0 40.0 40.0 40.0 72-73 39.43447210075311 40.0 40.0 40.0 40.0 40.0 74-75 39.41945555941858 40.0 40.0 40.0 40.0 40.0 76-77 39.42200259396152 40.0 40.0 40.0 40.0 40.0 78-79 39.413607958819526 40.0 40.0 40.0 40.0 40.0 80-81 39.42829442220412 40.0 40.0 40.0 40.0 40.0 82-83 39.395523851227246 40.0 40.0 40.0 40.0 40.0 84-85 39.39328770494102 40.0 40.0 40.0 40.0 40.0 86-87 39.38330532590455 40.0 40.0 40.0 40.0 40.0 88-89 39.408196215916774 40.0 40.0 40.0 40.0 40.0 90-91 39.44585816060328 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.015664147454877764 1101 2 -0.011094956034341408 1101 3 0.020830622256958975 1101 4 -0.12145310359822759 1101 5 -0.0846053508749236 1101 6 0.037501295090109465 1101 7 0.0040533121574171105 1101 8 -0.1187088764547255 1101 9 -0.09826355766273309 1101 10-11 -0.06738177563972414 1101 12-13 0.08237086190165144 1101 14-15 0.0953820469151907 1101 16-17 0.0454524862157939 1101 18-19 0.011961682973741006 1101 20-21 -0.03806844527230879 1101 22-23 -0.023836300206028227 1101 24-25 -0.04276397445237734 1101 26-27 -0.018453593000650415 1101 28-29 0.11640625381002678 1101 30-31 0.03414679692070166 1101 32-33 0.036448104576827234 1101 34-35 -9.970424372980347E-4 1101 36-37 -0.04267373103614602 1101 38-39 -0.04991239568886385 1101 40-41 -0.10044672414164779 1101 42-43 0.005784022290946211 1101 44-45 0.08408476956208233 1101 46-47 0.013398853489889007 1101 48-49 -0.04034626825452392 1101 50-51 -0.009866850867751964 1101 52-53 -0.030220282789095165 1101 54-55 -0.04822376759030789 1101 56-57 0.021192323301200133 1101 58-59 -0.10855616618097486 1101 60-61 -0.1397995391787461 1101 62-63 -0.14203055805472786 1101 64-65 -0.26611832483343534 1101 66-67 -0.1911354705509325 1101 68-69 -0.15464179441811154 1101 70-71 0.011463455101527131 1101 72-73 -0.03479320826504306 1101 74-75 -0.09571412575945004 1101 76-77 -0.07688995868122817 1101 78-79 -0.3523013856454824 1101 80-81 -0.26825614778567086 1101 82-83 0.11705362353717419 1101 84-85 -0.1734742779623275 1101 86-87 0.01149878602698351 1101 88-89 -0.18319330141881096 1101 90-91 0.1181228214224248 1102 1 0.1557210445152748 1102 2 0.050668818593749165 1102 3 0.1086484726134529 1102 4 0.10278624056540053 1102 5 -0.1342483089882549 1102 6 0.09727799502869061 1102 7 0.13722292093513033 1102 8 -0.02839361908659299 1102 9 0.018260061949796125 1102 10-11 0.019590889791238908 1102 12-13 0.0585870020514605 1102 14-15 1.6607334396212536E-4 1102 16-17 0.06197809798938181 1102 18-19 0.006108120375166948 1102 20-21 -0.1322730792084741 1102 22-23 0.06962657163401786 1102 24-25 -0.016319943793440927 1102 26-27 0.011926439272357925 1102 28-29 0.11818478738451432 1102 30-31 -0.01220867158259864 1102 32-33 0.05037450068153504 1102 34-35 0.1140162701172116 1102 36-37 0.002645866712185807 1102 38-39 0.09859440159745247 1102 40-41 0.08635895453314646 1102 42-43 -0.052347014913976864 1102 44-45 -0.004007966045726619 1102 46-47 -0.010730513987638801 1102 48-49 -0.011810911719393857 1102 50-51 0.007585476328387131 1102 52-53 0.08057858717578625 1102 54-55 0.024276297000653813 1102 56-57 0.04127226459055322 1102 58-59 -0.010023758254689596 1102 60-61 0.04849125581748126 1102 62-63 0.20188173961072664 1102 64-65 0.011001235044751922 1102 66-67 -0.001888071554930093 1102 68-69 0.19298539203420262 1102 70-71 -0.1276677022217072 1102 72-73 -0.07500934855558228 1102 74-75 -0.12267211079119278 1102 76-77 0.24428277367871942 1102 78-79 -0.06434940885778673 1102 80-81 0.08942016484152049 1102 82-83 0.1600107766425225 1102 84-85 -0.023075400016885794 1102 86-87 0.22581459192493725 1102 88-89 0.2184085990961364 1102 90-91 0.05463075793036154 1103 1 0.028031639718228973 1103 2 0.05801215121937986 1103 3 0.02844851524664449 1103 4 -0.008625657171329237 1103 5 0.060264995515694864 1103 6 0.11890387465869878 1103 7 0.20053534919453142 1103 8 0.029891680706349177 1103 9 -0.05151654951443163 1103 10-11 0.03468850827028547 1103 12-13 -0.017675151932969868 1103 14-15 -0.08652626152396437 1103 16-17 -0.005344755324664163 1103 18-19 -0.08076021525143773 1103 20-21 -0.11833655311315994 1103 22-23 -0.08920642372375198 1103 24-25 -0.08357786478875084 1103 26-27 0.017816484079922645 1103 28-29 0.03637604638372238 1103 30-31 -0.06375980642437895 1103 32-33 -0.011204140254442052 1103 34-35 0.0348522600736203 1103 36-37 -0.03461115015549865 1103 38-39 -0.0800204958298707 1103 40-41 -0.04486801098333615 1103 42-43 -0.03735184846506456 1103 44-45 0.10423657597011982 1103 46-47 -0.06556731427625095 1103 48-49 -0.06451364998734732 1103 50-51 0.0995385367327799 1103 52-53 -0.043940836844576836 1103 54-55 -0.010130146232512516 1103 56-57 -0.0852399891286737 1103 58-59 -0.05746484153006293 1103 60-61 -0.1370207221278008 1103 62-63 0.024415419061199373 1103 64-65 -0.12903224587297046 1103 66-67 -0.09301226406263652 1103 68-69 -0.09732463099173572 1103 70-71 -0.057817965168915464 1103 72-73 -0.07960460051591411 1103 74-75 0.1676528447341994 1103 76-77 0.030184508634945928 1103 78-79 0.11929009239226218 1103 80-81 -0.08908489839644318 1103 82-83 0.02232086871306649 1103 84-85 -0.10237447682609968 1103 86-87 -0.038618657452623495 1103 88-89 -0.41786232314058225 1103 90-91 -0.8539551006555044 1104 1 -0.10461668827911552 1104 2 0.009694861544780053 1104 3 -0.06343528511956009 1104 4 -0.15089032935600954 1104 5 -0.12087108826111148 1104 6 -0.006471803939881227 1104 7 0.06780478629232078 1104 8 0.09851156913243386 1104 9 0.021968904116008048 1104 10-11 0.03386701583526275 1104 12-13 -0.0991082664729177 1104 14-15 0.005052584403365756 1104 16-17 -0.012682507356444717 1104 18-19 -0.020127391960123475 1104 20-21 -0.04091153357165922 1104 22-23 -0.014080555064033717 1104 24-25 0.024298911345994156 1104 26-27 0.09977213291118403 1104 28-29 -0.008701530684732006 1104 30-31 -0.03591386837896238 1104 32-33 0.038749573670074255 1104 34-35 0.044032898855604685 1104 36-37 -0.03511881103850811 1104 38-39 0.014896967884148182 1104 40-41 -0.0026630197516226417 1104 42-43 -0.08281255928643816 1104 44-45 -0.1504772416360396 1104 46-47 -0.05478680438186245 1104 48-49 0.06565164077256469 1104 50-51 -0.05773757465986762 1104 52-53 -0.051872910125347005 1104 54-55 -0.009808900344324911 1104 56-57 0.04009851153423227 1104 58-59 -0.0797213770577585 1104 60-61 -0.06943792131195181 1104 62-63 -0.004701093333615347 1104 64-65 0.06480467119828148 1104 66-67 -0.11963786395690335 1104 68-69 -0.10654151154943747 1104 70-71 -0.2082441150327199 1104 72-73 -0.004590999064440382 1104 74-75 -0.1158185798036655 1104 76-77 -0.1988594501275429 1104 78-79 -0.1965003594789252 1104 80-81 0.025268048214478256 1104 82-83 0.05262109678388782 1104 84-85 -0.22836031278948354 1104 86-87 -0.3172661371888452 1104 88-89 0.14881152949907062 1104 90-91 0.09907520237480583 1201 1 0.10119650860012541 1201 2 -0.019082631953644125 1201 3 0.06818226439242636 1201 4 0.012868054252649586 1201 5 -0.07439078250287423 1201 6 0.0121971369615963 1201 7 0.03512824355039612 1201 8 -0.13289771943216522 1201 9 0.06476494947435185 1201 10-11 0.0064902825208790205 1201 12-13 -0.03428583577917266 1201 14-15 0.08997756624108177 1201 16-17 0.04592283920113971 1201 18-19 0.026701629803049798 1201 20-21 0.02618260500926084 1201 22-23 -0.05653041134502246 1201 24-25 -0.04818010989072974 1201 26-27 0.07631129502411937 1201 28-29 -0.05618165745225667 1201 30-31 -0.057775161112992635 1201 32-33 0.01878314107651846 1201 34-35 -0.08252675886324568 1201 36-37 -0.033053219875100126 1201 38-39 0.08368691698883879 1201 40-41 0.08172847713390041 1201 42-43 0.14235500699740555 1201 44-45 0.06525110946460444 1201 46-47 0.09096160558807753 1201 48-49 0.13599804302088359 1201 50-51 0.06838781773844715 1201 52-53 0.05689830161694687 1201 54-55 0.07116847053163156 1201 56-57 0.17532814321664603 1201 58-59 0.010344814884689413 1201 60-61 0.13943997793063545 1201 62-63 0.17091758918511601 1201 64-65 0.19697727066493798 1201 66-67 0.08691267871859054 1201 68-69 0.12482891128178153 1201 70-71 0.2814956896896561 1201 72-73 0.015663852291844194 1201 74-75 0.15321876195738326 1201 76-77 0.022179475015860817 1201 78-79 -0.11810770223105038 1201 80-81 0.19793733396036117 1201 82-83 -0.14643586172570622 1201 84-85 0.22909299616259204 1201 86-87 -0.14313742743275526 1201 88-89 0.11404617408153683 1201 90-91 -0.8960467488447037 1202 1 0.03805310094804781 1202 2 -0.025412653890604986 1202 3 -0.06623707349511676 1202 4 0.019575925700408447 1202 5 0.11373823684001394 1202 6 0.06227846218742883 1202 7 0.036652987271672544 1202 8 0.03056181396956248 1202 9 0.022445804265039726 1202 10-11 -0.07902632033676582 1202 12-13 0.04614189320346895 1202 14-15 0.10471619947681887 1202 16-17 -0.08015962040732916 1202 18-19 -0.007187217380597133 1202 20-21 -0.06126495064638959 1202 22-23 0.047903690198900506 1202 24-25 0.06657270312798147 1202 26-27 0.03244405115687954 1202 28-29 0.018268875957815567 1202 30-31 -0.01486213322886698 1202 32-33 0.012337541632071236 1202 34-35 0.024956260239441974 1202 36-37 -0.2016717413956428 1202 38-39 -0.11428998757872932 1202 40-41 -0.19022588324864387 1202 42-43 -0.11080973990445386 1202 44-45 -0.13918762770494197 1202 46-47 -0.03639156940363364 1202 48-49 -0.02422200471024638 1202 50-51 -0.05781861998040938 1202 52-53 -0.09426792371623804 1202 54-55 0.040828774425271774 1202 56-57 -0.045653634661469766 1202 58-59 -0.03593234571818016 1202 60-61 -0.13595471146930294 1202 62-63 0.08121096686620888 1202 64-65 0.0015406332920591126 1202 66-67 -0.1571484447132363 1202 68-69 -0.1730422878705582 1202 70-71 -0.17332466019788484 1202 72-73 -0.23969611790193568 1202 74-75 -0.07750289764412344 1202 76-77 0.05680943201668498 1202 78-79 -0.1570640993840371 1202 80-81 -0.23348070108698238 1202 82-83 -0.18868894408890924 1202 84-85 -0.20705609608950226 1202 86-87 0.1438158154028386 1202 88-89 0.18022780492943014 1202 90-91 -0.37049001501650025 1203 1 0.009225097649235181 1203 2 -0.03615630408261694 1203 3 -0.059274568392780225 1203 4 -0.10669049549866116 1203 5 -0.08357605887069042 1203 6 -0.007508197592812849 1203 7 -0.11039098863696495 1203 8 0.004012993963399936 1203 9 -0.11927674515475672 1203 10-11 -0.032547142042318455 1203 12-13 -0.13728237976538082 1203 14-15 -0.16589516478431676 1203 16-17 -0.1061228169285755 1203 18-19 -0.12277663117399129 1203 20-21 -0.03089250077426442 1203 22-23 -0.053272554979912456 1203 24-25 -0.08853171778785196 1203 26-27 -0.1322269417373363 1203 28-29 -0.07728521715881698 1203 30-31 -0.09166461234051582 1203 32-33 -0.05432047139118623 1203 34-35 0.03485490879177888 1203 36-37 -0.025340166324895108 1203 38-39 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2401 24-25 0.07679490791212373 2401 26-27 -0.021036808366282855 2401 28-29 -0.0028817760085502186 2401 30-31 0.03295047712150989 2401 32-33 0.013370351535847647 2401 34-35 -0.06889105466098755 2401 36-37 0.013031735736888095 2401 38-39 0.03920293191838198 2401 40-41 -0.006433733971356048 2401 42-43 0.011163523554827748 2401 44-45 0.09268592633327444 2401 46-47 -0.03778068161969372 2401 48-49 0.0948314403507382 2401 50-51 0.1067066450671561 2401 52-53 0.06262539305374304 2401 54-55 0.05574872984902868 2401 56-57 0.07248142270002944 2401 58-59 -0.060675359330574 2401 60-61 0.10114852945408614 2401 62-63 0.08934174770942604 2401 64-65 0.07372780233662724 2401 66-67 0.12631190305079087 2401 68-69 0.16166458676497086 2401 70-71 0.1305068872342332 2401 72-73 -0.0012649226251468804 2401 74-75 0.15583946343273425 2401 76-77 0.12077146418901208 2401 78-79 0.09255787247540326 2401 80-81 0.18291888669303802 2401 82-83 0.1708053004501977 2401 84-85 0.13060306939827626 2401 86-87 0.007313445119052631 2401 88-89 0.36485095602236584 2401 90-91 -0.017053829883259652 2402 1 0.021924303687178792 2402 2 -0.03275253481538698 2402 3 0.026136947180788184 2402 4 -0.047886341761859796 2402 5 0.10275921076664929 2402 6 0.06443347188960757 2402 7 0.023590476095954216 2402 8 0.06682081249271477 2402 9 0.09432007991289737 2402 10-11 0.10053022714431137 2402 12-13 0.09755218612320249 2402 14-15 0.07420565953231772 2402 16-17 0.04188717522437457 2402 18-19 0.023950487342013105 2402 20-21 -0.05029784533966364 2402 22-23 0.019939558461189222 2402 24-25 0.06734887748906004 2402 26-27 0.013871496735262667 2402 28-29 0.04688268947728602 2402 30-31 0.03279179367034857 2402 32-33 -0.02636196950909664 2402 34-35 -0.05968017302529205 2402 36-37 -0.007731941913242224 2402 38-39 -0.3916514070417989 2402 40-41 0.06477026987401757 2402 42-43 0.10946100427793226 2402 44-45 0.0794569722199725 2402 46-47 0.14766850653980157 2402 48-49 0.09964129126544208 2402 50-51 0.1726371765064414 2402 52-53 0.09614158013909702 2402 54-55 0.25505068043479184 2402 56-57 0.10283411395000286 2402 58-59 0.12775199914256063 2402 60-61 0.2494276136831033 2402 62-63 0.10773875345599038 2402 64-65 0.1202143002074294 2402 66-67 0.16760609122101755 2402 68-69 0.13925128812157084 2402 70-71 0.15917216637458154 2402 72-73 0.16022421082666227 2402 74-75 0.2918310411230678 2402 76-77 0.24551009650363653 2402 78-79 0.18631914535317406 2402 80-81 0.24696377233891553 2402 82-83 0.29562689068187353 2402 84-85 0.43553711552154084 2402 86-87 0.0859054717782115 2402 88-89 0.3704534524400316 2402 90-91 0.02848696708068843 2403 1 -0.07634828713754871 2403 2 0.08899399209499137 2403 3 0.05971066297893657 2403 4 0.2744684016455139 2403 5 0.09969438595405933 2403 6 0.11747589633223754 2403 7 0.15349404095483266 2403 8 0.18009648982003057 2403 9 0.04353524114394958 2403 10-11 0.11730366546689908 2403 12-13 7.583293988560058E-4 2403 14-15 0.06026065502127409 2403 16-17 0.038479635074025964 2403 18-19 0.06204092290017371 2403 20-21 0.07763562246471167 2403 22-23 0.09329251190351329 2403 24-25 -0.008331340197109682 2403 26-27 0.056180840683133226 2403 28-29 0.03306723549301438 2403 30-31 0.10232068395395544 2403 32-33 0.04444571975422207 2403 34-35 0.06525363855890731 2403 36-37 -0.024620412747637488 2403 38-39 0.20506558568615674 2403 40-41 0.021284939509243372 2403 42-43 0.14599620288570492 2403 44-45 0.1416407389539387 2403 46-47 0.02975638148191706 2403 48-49 0.051931259981451205 2403 50-51 0.05424987713583107 2403 52-53 0.08221202946283768 2403 54-55 0.1679856825619055 2403 56-57 0.0928134861468024 2403 58-59 0.10949661635979879 2403 60-61 0.10523179143481087 2403 62-63 0.08986427851893097 2403 64-65 -0.03680686252877763 2403 66-67 0.04838234986867462 2403 68-69 0.14749786820780741 2403 70-71 0.009209620582360856 2403 72-73 0.15026888928017712 2403 74-75 0.12904183289794702 2403 76-77 0.08924864240008645 2403 78-79 0.1511505378155178 2403 80-81 0.03823162751510267 2403 82-83 0.07517460971016732 2403 84-85 0.05134347736066047 2403 86-87 0.2515200769532484 2403 88-89 -0.09333822895503374 2403 90-91 -0.11186391011229802 2404 1 0.03219772175933855 2404 2 -0.1339359824139308 2404 3 -0.0231843237757019 2404 4 0.08611432557214016 2404 5 0.0605011036028813 2404 6 -0.04341979017748798 2404 7 -0.10032312303777502 2404 8 -0.054947244872828094 2404 9 0.14006645521902072 2404 10-11 0.03755857624813075 2404 12-13 -0.031202984141401657 2404 14-15 0.02483805293201158 2404 16-17 -0.07712290403728161 2404 18-19 0.003705840179122788 2404 20-21 0.03440925169447695 2404 22-23 -0.009870880909005564 2404 24-25 0.054284744173351385 2404 26-27 0.03878043082658422 2404 28-29 7.540451096446077E-4 2404 30-31 -0.05534313370986155 2404 32-33 -0.0782036314573773 2404 34-35 0.007212078738625394 2404 36-37 0.11280389661264678 2404 38-39 0.031946861244975366 2404 40-41 0.05246310974797552 2404 42-43 0.002556439697414703 2404 44-45 -0.06006132096699446 2404 46-47 0.13450580352331798 2404 48-49 0.04723229409389518 2404 50-51 0.07125046186224893 2404 52-53 -0.008601857662490886 2404 54-55 0.05220267502687648 2404 56-57 0.06430006451849835 2404 58-59 0.0812220414673277 2404 60-61 0.1163063818048542 2404 62-63 0.1955236960979576 2404 64-65 0.06829314855805535 2404 66-67 0.2475494222990804 2404 68-69 0.10583503642831005 2404 70-71 0.1401235137537853 2404 72-73 0.15806221661615183 2404 74-75 0.20184271062949932 2404 76-77 0.21939134192065524 2404 78-79 0.22252155822202724 2404 80-81 0.08579817300403647 2404 82-83 0.10212173072510211 2404 84-85 0.13359301915268418 2404 86-87 0.008823950604643471 2404 88-89 -0.18850731108064167 2404 90-91 -0.16321987959241113 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 17.0 31 330.0 32 2147.0 33 3774.0 34 5152.0 35 6516.0 36 9207.0 37 13814.0 38 34243.0 39 243244.0 40 229101.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 27.199408267813602 15.465030271484537 24.315992292870906 33.01956916783095 2 26.34051881622214 17.95907928388747 26.88472780416514 28.815674095725246 3 26.88454830196605 19.67911313225397 20.965582737491893 32.470755828288084 4 27.394278095864266 21.796382032527006 19.32681332127953 31.482526550329197 5 26.967646494808644 22.18666958880092 20.420969965938873 30.424713950451558 6 27.123432777214656 24.968176131642146 22.84561086303409 25.062780228109105 7 25.458181519327177 25.12578874795679 22.666082239815903 26.749947492900127 8 24.630852258718463 26.186158215306506 23.10969874622177 26.07329077975326 9 24.528029659662675 25.615611502250957 23.90963299820106 25.946725839885303 10-11 24.613410769891058 24.36311170771352 23.87027550247012 27.153202019925303 12-13 25.138664402012623 24.027705485393895 23.50026025258198 27.33336986001151 14-15 25.287054032088683 23.55797240409464 23.527929211297703 27.627044352518972 16-17 25.36102055538814 23.46181592380535 23.403829822206394 27.773333698600116 18-19 25.517628688053033 23.754668566053933 23.2536138582217 27.474088887671332 20-21 25.4108794710937 23.70207014948543 23.457889305901798 27.42916107351907 22-23 25.373348309271382 23.339725502013533 23.484097197490616 27.802828991224466 24-25 25.463203937575905 23.618880639947402 23.407756440109946 27.510158982366743 26-27 25.652412130509823 23.627099142536228 23.39204996849574 27.328438758458205 28-29 25.578993507382954 23.53815668118602 23.436521199170844 27.446328612260178 30-31 25.672183422702105 23.64266616016247 23.421040135587276 27.26411028154815 32-33 25.698956478414516 23.643191275384513 23.506915291143446 27.150936955057524 34-35 25.7556797928822 23.420257771607602 23.53010149522541 27.29396094028479 36-37 25.857620923379272 23.743164410679242 23.378312889094506 27.020901776846983 38-39 26.090518244222128 23.61572465624065 23.42382941950469 26.869927680032536 40-41 25.84876952833505 23.515335696549432 23.470261639874312 27.1656331352412 42-43 26.020569082059875 23.637437460961415 23.440981906711443 26.901011550267267 44-45 25.973006020064044 23.694810315305777 23.56157502838699 26.770608636243193 46-47 25.97276564960587 23.69287028173922 23.58204050304589 26.75232356560902 48-49 26.070138892075306 23.714089722353503 23.548217620526845 26.66755376504435 50-51 26.195064404668518 23.701277314346285 23.536597780718722 26.567060500266482 52-53 26.08151855879568 23.699226706381996 23.51199371118149 26.707261023640832 54-55 26.35377533202815 23.84425101857241 23.25466955923594 26.547304090163497 56-57 26.39217731931658 23.809137555608075 23.361056375157506 26.437628749917835 58-59 26.254084724615296 23.737151684391897 23.35036539718377 26.65839819380904 60-61 26.7151781989935 23.91297107230259 23.027170370323375 26.34468035838053 62-63 26.673356799912767 23.85259671445171 23.05080298636644 26.423243499269084 64-65 26.550317550472453 23.73293304516519 23.204419889502763 26.51232951485959 66-67 26.792849293989317 23.79644714612583 23.005763393511813 26.40494016637304 68-69 26.87344880334684 23.802354953469614 22.92023928654873 26.403956956634822 70-71 26.74353356787009 23.791981185012418 22.969557389127708 26.49492785798978 72-73 27.01618183008533 23.874473517665802 22.58500098203959 26.524343670209284 74-75 27.134048489038122 23.67358779379814 22.687882678254 26.504481038909738 76-77 27.160769866873935 23.58116573148355 22.603748540440016 26.654315861202498 78-79 27.65943324607873 23.79644648361176 22.069054207096247 26.475066063213266 80-81 27.788760237221123 23.434256355375872 22.105461337614017 26.67152206978899 82-83 28.261227576868414 23.117554849373892 21.847647744115548 26.773569829642145 84-85 29.116603865993635 22.692766468390328 20.760559597443738 27.430070068172302 86-87 29.776612041128953 21.928828838757315 20.29226683124125 28.002292288872482 88-89 29.201833076773358 21.825291505210824 19.893129500858148 29.079745917157666 90-91 26.115107913669068 23.004655099449852 20.15234870926788 30.727888277613204 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 3.0 8 3.5 9 3.5 10 5.0 11 5.5 12 6.5 13 11.5 14 22.0 15 35.0 16 48.5 17 69.5 18 108.5 19 144.5 20 180.5 21 251.5 22 377.0 23 548.5 24 744.5 25 940.5 26 1192.5 27 1511.0 28 1921.5 29 2408.5 30 2965.0 31 3627.5 32 4391.5 33 5258.0 34 6368.0 35 7543.0 36 8816.5 37 10391.5 38 12046.0 39 13870.5 40 15709.0 41 17520.5 42 19481.5 43 21371.0 44 23309.0 45 25297.5 46 27123.5 47 28552.5 48 29801.0 49 30902.0 50 31722.5 51 32457.5 52 32996.5 53 33322.0 54 33447.0 55 33430.0 56 32999.5 57 32364.0 58 31722.0 59 30771.0 60 29857.5 61 28716.0 62 27310.5 63 25831.0 64 24040.0 65 21981.0 66 19700.0 67 17759.0 68 15799.5 69 13535.0 70 11488.5 71 9663.0 72 7912.0 73 6292.5 74 4898.0 75 3813.5 76 2985.0 77 2244.0 78 1625.5 79 1172.5 80 848.0 81 600.0 82 417.5 83 290.5 84 199.5 85 138.5 86 92.0 87 54.5 88 36.0 89 27.0 90 15.0 91 8.0 92 7.5 93 8.0 94 6.5 95 6.0 96 5.5 97 4.5 98 3.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.026481841675113464 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.06994441568083465 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 1.703766175130125E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 2.4396967944823815E-4 72-73 0.0 74-75 0.0 76-77 3.5490694339944065E-4 78-79 4.148379228235528E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 3634.0 32-33 8540.0 34-35 9613.0 36-37 10955.0 38-39 12317.0 40-41 13707.0 42-43 14943.0 44-45 16233.0 46-47 17204.0 48-49 18193.0 50-51 19152.0 52-53 19877.0 54-55 20518.0 56-57 20742.0 58-59 21287.0 60-61 21640.0 62-63 22360.0 64-65 22174.0 66-67 22010.0 68-69 22145.0 70-71 21572.0 72-73 21429.0 74-75 21240.0 76-77 20489.0 78-79 19777.0 80-81 19187.0 82-83 18723.0 84-85 18116.0 86-87 17358.0 88-89 16497.0 90-91 15913.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.80573033780875 #Duplication Level Percentage of deduplicated Percentage of total 1 99.8175231934862 99.62360802837054 2 0.17195803907576143 0.3432479535482765 3 0.008866537567764168 0.02654793772554964 4 0.0016522298702963986 0.006596080355635004 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0029221342538056235 0.0 0.0 0.0 0.0 2 0.0029221342538056235 0.0 0.0 0.0 0.0 3 0.0029221342538056235 0.0 0.0 0.0 0.0 4 0.0029221342538056235 0.0 0.0 0.0 0.0 5 0.0029221342538056235 0.0 0.0 0.0 0.0 6 0.003104767644668475 0.0 0.0 0.0 0.0 7 0.0034700344263941777 0.0 0.0 0.0 0.0 8 0.009131669543142573 0.0 0.0 0.0 0.0 9 0.009131669543142573 0.0 0.0 0.0 0.0 10-11 0.009131669543142573 0.0 0.0 0.0 0.0 12-13 0.009131669543142573 0.0 0.0 0.0 0.0 14-15 0.009131669543142573 0.0 0.0 0.0 0.0 16-17 0.009131669543142573 0.0 0.0 0.0 0.0 18-19 0.009131669543142573 0.0 0.0 0.0 0.0 20-21 0.009314302934005423 0.0 9.131669543142573E-5 0.0 9.131669543142573E-5 22-23 0.009314302934005423 0.0 1.8263339086285147E-4 0.0 1.8263339086285147E-4 24-25 0.0095882530202997 0.0 1.8263339086285147E-4 0.0 1.8263339086285147E-4 26-27 0.009679569715731128 0.0 1.8263339086285147E-4 0.0 1.8263339086285147E-4 28-29 0.01004483649745683 0.0 1.8263339086285147E-4 0.0 1.8263339086285147E-4 30-31 0.010136153192888256 0.0 1.8263339086285147E-4 0.0 1.8263339086285147E-4 32-33 0.010410103279182533 0.0 1.8263339086285147E-4 0.0 1.8263339086285147E-4 34-35 0.010410103279182533 0.0 1.8263339086285147E-4 0.0 1.8263339086285147E-4 36-37 0.010410103279182533 0.0 1.8263339086285147E-4 0.0 1.8263339086285147E-4 38-39 0.010592736670045384 0.0 1.8263339086285147E-4 0.0 1.8263339086285147E-4 40-41 0.010775370060908236 0.0 1.8263339086285147E-4 0.0 1.8263339086285147E-4 42-43 0.011140636842633939 0.0 1.8263339086285147E-4 0.0 1.8263339086285147E-4 44-45 0.011140636842633939 0.0 1.8263339086285147E-4 0.0 3.6526678172570294E-4 46-47 0.01132327023349679 0.0 1.8263339086285147E-4 0.0 3.6526678172570294E-4 48-49 0.01132327023349679 0.0 1.8263339086285147E-4 0.0 3.6526678172570294E-4 50-51 0.01132327023349679 0.0 1.8263339086285147E-4 0.0 3.6526678172570294E-4 52-53 0.01132327023349679 0.0 1.8263339086285147E-4 0.0 3.6526678172570294E-4 54-55 0.011688537015222494 0.0 1.8263339086285147E-4 0.0 3.6526678172570294E-4 56-57 0.011688537015222494 0.0 1.8263339086285147E-4 0.0 3.6526678172570294E-4 58-59 0.011688537015222494 0.0 1.8263339086285147E-4 0.0 3.6526678172570294E-4 60-61 0.01177985371065392 0.0 1.8263339086285147E-4 0.0 3.6526678172570294E-4 62-63 0.011962487101516771 0.0 1.8263339086285147E-4 0.0 3.6526678172570294E-4 64-65 0.012145120492379622 0.0 1.8263339086285147E-4 0.0 3.6526678172570294E-4 66-67 0.012327753883242473 0.0 1.8263339086285147E-4 0.0 3.6526678172570294E-4 68-69 0.0124190705786739 0.0 1.8263339086285147E-4 0.0 3.6526678172570294E-4 70-71 0.0124190705786739 0.0 1.8263339086285147E-4 0.0 3.6526678172570294E-4 72-73 0.0124190705786739 0.0 1.8263339086285147E-4 0.0 3.6526678172570294E-4 74-75 0.0124190705786739 0.0 1.8263339086285147E-4 0.0 3.6526678172570294E-4 76-77 0.0124190705786739 0.0 1.8263339086285147E-4 0.0 3.6526678172570294E-4 78-79 0.0124190705786739 0.0 1.8263339086285147E-4 0.0 5.479001725885544E-4 80 0.0124190705786739 0.0 1.8263339086285147E-4 0.0 5.479001725885544E-4 >>END_MODULE