##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-26B_S17_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1057931
Sequences flagged as poor quality	0
Sequence length	61
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.512002200521586	40.0	40.0	40.0	40.0	40.0
2	39.20055466755393	40.0	40.0	40.0	40.0	40.0
3	39.14704456150732	40.0	40.0	40.0	40.0	40.0
4	39.03148693062213	40.0	40.0	40.0	40.0	40.0
5	39.08213673670589	40.0	40.0	40.0	40.0	40.0
6	39.16954886471802	40.0	40.0	40.0	40.0	40.0
7	39.22046333834626	40.0	40.0	40.0	40.0	40.0
8	39.21444782315671	40.0	40.0	40.0	40.0	40.0
9	39.24516060121123	40.0	40.0	40.0	40.0	40.0
10	39.25217807210489	40.0	40.0	40.0	40.0	40.0
11	39.24019997523468	40.0	40.0	40.0	40.0	40.0
12	39.25640140992182	40.0	40.0	40.0	40.0	40.0
13	39.09809240867315	40.0	40.0	40.0	40.0	40.0
14	39.24743674209377	40.0	40.0	40.0	40.0	40.0
15	39.16594182418324	40.0	40.0	40.0	40.0	40.0
16	39.34165838792889	40.0	40.0	40.0	40.0	40.0
17	39.28820688683856	40.0	40.0	40.0	40.0	40.0
18	39.37179267835048	40.0	40.0	40.0	40.0	40.0
19	39.326148869822326	40.0	40.0	40.0	40.0	40.0
20	39.332557605363675	40.0	40.0	40.0	40.0	40.0
21	39.319101151209296	40.0	40.0	40.0	40.0	40.0
22	39.37980832398332	40.0	40.0	40.0	40.0	40.0
23	39.371221752647386	40.0	40.0	40.0	40.0	40.0
24	39.37526738511302	40.0	40.0	40.0	40.0	40.0
25	39.330655780008335	40.0	40.0	40.0	40.0	40.0
26	39.29951575291772	40.0	40.0	40.0	40.0	40.0
27	39.31003817829329	40.0	40.0	40.0	40.0	40.0
28	39.32745708368504	40.0	40.0	40.0	40.0	40.0
29	39.33351418948873	40.0	40.0	40.0	40.0	40.0
30	39.33925747520396	40.0	40.0	40.0	40.0	40.0
31	39.2861954135005	40.0	40.0	40.0	40.0	40.0
32	39.302858125908024	40.0	40.0	40.0	40.0	40.0
33	39.30523635284343	40.0	40.0	40.0	40.0	40.0
34	39.307236483286715	40.0	40.0	40.0	40.0	40.0
35	39.30703987311082	40.0	40.0	40.0	40.0	40.0
36	39.276599324530615	40.0	40.0	40.0	40.0	40.0
37	39.27400180162979	40.0	40.0	40.0	40.0	40.0
38	39.26788041942244	40.0	40.0	40.0	40.0	40.0
39	38.06057672948425	40.0	40.0	40.0	40.0	40.0
40	39.25770584281962	40.0	40.0	40.0	40.0	40.0
41	39.27771092821744	40.0	40.0	40.0	40.0	40.0
42	39.17619580104941	40.0	40.0	40.0	40.0	40.0
43	39.26588028897915	40.0	40.0	40.0	40.0	40.0
44	39.22008902281907	40.0	40.0	40.0	40.0	40.0
45	39.07254820966585	40.0	40.0	40.0	40.0	40.0
46	39.07658628020164	40.0	40.0	40.0	40.0	40.0
47	39.07149710141777	40.0	40.0	40.0	40.0	40.0
48	39.237814186369434	40.0	40.0	40.0	40.0	40.0
49	39.222187458350305	40.0	40.0	40.0	40.0	40.0
50	39.20395564550051	40.0	40.0	40.0	40.0	40.0
51	39.1631287862819	40.0	40.0	40.0	40.0	40.0
52	39.16302670022903	40.0	40.0	40.0	40.0	40.0
53	39.0759359542352	40.0	40.0	40.0	40.0	40.0
54	38.98152904111894	40.0	40.0	40.0	40.0	40.0
55	39.042047165646906	40.0	40.0	40.0	40.0	40.0
56	39.048758378381955	40.0	40.0	40.0	40.0	40.0
57	38.95416997894948	40.0	40.0	40.0	40.0	40.0
58	38.89809070723894	40.0	40.0	40.0	40.0	40.0
59	38.81141964835136	40.0	40.0	40.0	40.0	40.0
60	38.78589813513358	40.0	40.0	40.0	40.0	40.0
61	38.66057427185705	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.019804545014281416
1101	2	0.0520695479342308
1101	3	0.07372273900956827
1101	4	-0.06639758637551552
1101	5	-0.11724821697668375
1101	6	0.1426715925313573
1101	7	0.08653052092589775
1101	8	0.09837681351518768
1101	9	0.007289952245514542
1101	10	0.0215389548042495
1101	11	0.026929700778858034
1101	12	-0.06327515887701196
1101	13	0.12457736594402746
1101	14	0.0707967810527137
1101	15	-0.05236833104840599
1101	16	0.07175703791995147
1101	17	-0.02874808834097564
1101	18	-0.04530267208233596
1101	19	0.11117394015965232
1101	20	0.06360720235706907
1101	21	0.09974170796395754
1101	22	0.03798202660924943
1101	23	-0.07570233145808913
1101	24	0.06813485617518467
1101	25	0.1070866499840264
1101	26	0.08644212576988508
1101	27	0.06646566571796342
1101	28	0.10008374599628667
1101	29	0.08368814073373443
1101	30	0.1966361644253709
1101	31	0.01771634802221911
1101	32	0.12533556586703298
1101	33	0.10970303499014733
1101	34	0.004412363388361484
1101	35	-0.00538480946213582
1101	36	-0.052252420790658505
1101	37	0.029115387254961433
1101	38	0.03110238687202127
1101	39	1.3504618223640037
1101	40	0.030679867518728088
1101	41	-0.05222956241992449
1101	42	0.09013982481415184
1101	43	0.004583476466152092
1101	44	-0.024228227847260086
1101	45	-0.0033178685135411
1101	46	0.03987296134518914
1101	47	0.06748025014394443
1101	48	-0.014259289856845214
1101	49	-0.016825363506157487
1101	50	-0.012849646975034545
1101	51	-0.027991199802507083
1101	52	0.05556228291822407
1101	53	0.0016873177502247927
1101	54	0.1706341757926566
1101	55	-0.05063826117940806
1101	56	0.132483287210853
1101	57	-0.0071765415347684325
1101	58	0.05266327367431245
1101	59	0.1380527454015663
1101	60	0.12077500767119176
1101	61	0.04274866078298345
1102	1	0.044456617497218076
1102	2	0.08388512905412426
1102	3	0.08472061090250804
1102	4	-0.09178889240782695
1102	5	-0.06513277062767742
1102	6	-0.13199915969499187
1102	7	-0.09177143505078789
1102	8	-0.02257141177081934
1102	9	-0.027685099919395384
1102	10	-0.05808864029639693
1102	11	-0.08319361320134533
1102	12	-0.011156599237438058
1102	13	-0.0017143037206253098
1102	14	-0.002078778647749857
1102	15	-0.06798148324818953
1102	16	0.023075205146554367
1102	17	0.03834782038011042
1102	18	-0.07597826997249513
1102	19	0.01076997341236563
1102	20	-0.06488106676406602
1102	21	-0.04289162389344625
1102	22	-0.014255183993093112
1102	23	-0.0406048231974907
1102	24	-0.01242776385945632
1102	25	0.05472006486250791
1102	26	-0.06073176742072661
1102	27	-0.1075838812636647
1102	28	-0.09307483257209981
1102	29	0.042005331078932784
1102	30	-0.07212348677538927
1102	31	-0.023331350356656344
1102	32	-0.1533845396250868
1102	33	0.0033969611678017486
1102	34	-0.041487571301722426
1102	35	0.015591158702051189
1102	36	-0.11999347250172576
1102	37	0.043157019560119636
1102	38	-0.051030715293720164
1102	39	1.2173683046500727
1102	40	-0.04792137945791808
1102	41	0.0031915066702197237
1102	42	0.03634389340063393
1102	43	-0.03641129597306758
1102	44	-0.08242358475488487
1102	45	1.897721933588059E-4
1102	46	0.037225626598655026
1102	47	-0.0643489256781038
1102	48	-9.793759773799593E-4
1102	49	-0.04686585012039046
1102	50	-0.03811434585757212
1102	51	-0.016675772271611322
1102	52	0.0019545326235004268
1102	53	-0.017146014660653464
1102	54	0.016440504212980045
1102	55	-0.04562793889157035
1102	56	-0.07347956362239927
1102	57	-0.13926169902575936
1102	58	-0.0488085517378849
1102	59	0.008273152536951045
1102	60	-0.029778189470668792
1102	61	-0.08098492179809114
1103	1	-0.003942692914293389
1103	2	-0.033931768722375466
1103	3	-0.11650195100155969
1103	4	-0.05483634453712227
1103	5	-0.11619634541369805
1103	6	0.018890295977648464
1103	7	-0.048856321368823785
1103	8	-0.012479511697605972
1103	9	-0.06219414265344625
1103	10	-0.03273955614157842
1103	11	-3.351977929000327E-4
1103	12	-0.03155702584346898
1103	13	0.03883823839863254
1103	14	-0.07648635134396642
1103	15	-0.037975446808964364
1103	16	-0.10527201159519706
1103	17	0.04499195020341773
1103	18	-0.03764709615929007
1103	19	-0.07063439285062856
1103	20	0.0063160260330761275
1103	21	-0.03626815796135929
1103	22	-0.01228040478892467
1103	23	0.011845987485706644
1103	24	-0.0386275115361272
1103	25	0.02618460868171013
1103	26	-0.00599277518320207
1103	27	-0.15032025228659052
1103	28	-0.03951058255037765
1103	29	0.051031318763428146
1103	30	0.02254778644662281
1103	31	-0.002639218486244488
1103	32	-0.10340375273894864
1103	33	-0.05922186158792897
1103	34	-0.05365309285876663
1103	35	0.011683961270712473
1103	36	-0.09700351592775291
1103	37	-0.013599112858571516
1103	38	-0.03036062490728142
1103	39	1.26741934876636
1103	40	-0.04955761524428226
1103	41	-0.02070352833914768
1103	42	0.054661744169969495
1103	43	-0.027465897464324485
1103	44	0.0027781159177990844
1103	45	0.05599536561597063
1103	46	-0.08422053927229456
1103	47	-0.030882720654247464
1103	48	0.06431714268215671
1103	49	-0.02974202786532487
1103	50	0.02948688546926803
1103	51	-0.03360914232926859
1103	52	0.14000379069079116
1103	53	-0.03883552368057508
1103	54	-0.032281851697916863
1103	55	0.09467161692948167
1103	56	0.006930180676583575
1103	57	0.08898923259285851
1103	58	-0.07532170176471453
1103	59	0.09094765931354232
1103	60	0.09393514966519945
1103	61	0.08606576319699144
1104	1	0.05398740188767448
1104	2	0.011668521321631431
1104	3	0.04524367070002455
1104	4	0.016293825140522245
1104	5	0.09485123490483716
1104	6	0.11272930308263795
1104	7	0.16252058766542632
1104	8	-0.018592460227829122
1104	9	-0.022477582830184417
1104	10	0.06465682246803794
1104	11	0.15120641184607564
1104	12	-0.054090152247447065
1104	13	-0.1508910035144524
1104	14	0.08200637257129983
1104	15	-0.015960823267676005
1104	16	0.009809733298119738
1104	17	0.022630737531549983
1104	18	-0.02000142882979361
1104	19	0.023301962994224823
1104	20	0.06986576670828981
1104	21	0.03069396673357261
1104	22	-0.011046630334050178
1104	23	0.020200718699186382
1104	24	0.07429668149848112
1104	25	-0.04786188069230235
1104	26	0.01277102182154266
1104	27	-0.022435767267893425
1104	28	-0.01369418002990841
1104	29	-0.008860249584316193
1104	30	-0.035022601309457
1104	31	0.020771082201399338
1104	32	0.07629442646852169
1104	33	0.06650507157206675
1104	34	0.012087339671694508
1104	35	-0.06239981059942323
1104	36	-0.09843889202830525
1104	37	-0.036076315518364765
1104	38	-0.14109481650323374
1104	39	1.2309218563162787
1104	40	-0.1074034792216878
1104	41	-0.03139877180895212
1104	42	-0.024333651355455288
1104	43	0.0017856728234946218
1104	44	0.0025892869791235285
1104	45	-0.08316258677065491
1104	46	-0.05541428768810874
1104	47	-0.13606976011940475
1104	48	-0.045124934256655536
1104	49	0.026454776075432562
1104	50	-0.07067254035267467
1104	51	-0.009256768198035559
1104	52	0.004699398527954202
1104	53	0.012379234112223969
1104	54	0.0035065829093170464
1104	55	0.020235732596006528
1104	56	-0.06647456647257854
1104	57	0.002638829083196015
1104	58	-0.0033281661352901892
1104	59	-0.027396303990123272
1104	60	0.03078205332369066
1104	61	0.06149280096372678
1201	1	0.03921713110705127
1201	2	0.08273796016199952
1201	3	0.0798801841536374
1201	4	-0.08539475263097529
1201	5	0.14926908090019708
1201	6	-0.023448133175300256
1201	7	-0.057047122580286214
1201	8	-0.031751484542219544
1201	9	-0.08583349890521674
1201	10	0.008224321631935538
1201	11	-0.03157645632438033
1201	12	0.0022052647623240773
1201	13	-0.013094477685790196
1201	14	0.04145036125370183
1201	15	0.029260812208868003
1201	16	-0.012419329854132855
1201	17	0.006822448921667501
1201	18	0.08642063300472813
1201	19	0.039443072038189086
1201	20	-0.04498251738668557
1201	21	0.11039252553754864
1201	22	0.112575359650819
1201	23	-0.0040719846799675
1201	24	0.012289779396027711
1201	25	-0.044901279248946935
1201	26	0.09991916587448202
1201	27	0.08379226298159637
1201	28	0.007282373435465672
1201	29	0.11071197044647363
1201	30	0.020975800743251227
1201	31	-0.019261146137971252
1201	32	0.14998141618185912
1201	33	0.035535720975147456
1201	34	0.05968918685920244
1201	35	0.011600562289046934
1201	36	0.064832378194005
1201	37	0.05920887894245652
1201	38	0.07408962134523733
1201	39	1.324507143129665
1201	40	0.09110242642286437
1201	41	0.058920032485893614
1201	42	0.0652860936259998
1201	43	0.042005794666117424
1201	44	0.08874374379341532
1201	45	0.02031353681586978
1201	46	0.11589821066547756
1201	47	-0.023869717306283178
1201	48	0.03227525392669861
1201	49	0.0045063596621659485
1201	50	0.013393945644104122
1201	51	0.09751380027955037
1201	52	-0.0858032072156405
1201	53	-0.1058896665547735
1201	54	-0.016049686633969884
1201	55	-0.03635328932538329
1201	56	-0.03379269106012117
1201	57	0.08659551161709089
1201	58	-0.10372126764747946
1201	59	0.0983163848486015
1201	60	0.0659518978827407
1201	61	0.17797892987930197
1202	1	0.10126256762473673
1202	2	0.04444742344917074
1202	3	-0.028325467622757117
1202	4	0.08854963000958804
1202	5	0.030688329023234928
1202	6	-8.674739970686574E-4
1202	7	0.006563968106249263
1202	8	-0.023996827389893838
1202	9	0.06848976761375525
1202	10	0.083872273352263
1202	11	0.08691977612857471
1202	12	-0.10886825376000075
1202	13	-0.02449199017349457
1202	14	-0.06217863187372785
1202	15	-0.07776940612644978
1202	16	0.025473999646287382
1202	17	0.04302300431312034
1202	18	0.049729319667456195
1202	19	-0.06564319954504327
1202	20	-0.012073107469440458
1202	21	-0.007717235033069869
1202	22	0.03516715460396824
1202	23	-0.0017862113864524076
1202	24	-0.04292662398383129
1202	25	-0.01688172697526369
1202	26	-0.08321568171407989
1202	27	0.04263402992490484
1202	28	-0.00271920533965897
1202	29	-0.04490192130919013
1202	30	-0.04872205168804555
1202	31	-0.01642735980495047
1202	32	0.023769037443166496
1202	33	-0.008365303712103866
1202	34	0.03246370820619404
1202	35	-0.0584421839208531
1202	36	-0.12617027351772236
1202	37	-0.18138145515379023
1202	38	-0.1899466736129014
1202	39	1.1032254077686687
1202	40	-0.1362916309925737
1202	41	-0.10619172954216793
1202	42	-0.1284440135304834
1202	43	-0.07184152866251026
1202	44	-0.03692062190577161
1202	45	0.014996992402743103
1202	46	-0.016051264781587804
1202	47	-0.10135801132346245
1202	48	0.005895651121981871
1202	49	-0.07301260672662124
1202	50	-0.10072022731757357
1202	51	-0.07811449556263028
1202	52	-0.030075597642209573
1202	53	-0.042326288446311366
1202	54	0.04171490921876142
1202	55	-0.03798710432003105
1202	56	-0.04472023465387309
1202	57	-0.09666611510747458
1202	58	-0.05086288803649808
1202	59	-0.0866383567890665
1202	60	-0.138954199077574
1202	61	0.021108834103969798
1203	1	-0.043520480909229775
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2403	11	0.09521634967778425
2403	12	0.06494892052476331
2403	13	0.11503456158328618
2403	14	0.044018315902775385
2403	15	0.1469505968863558
2403	16	0.028944955270560513
2403	17	0.08271688215398854
2403	18	0.0356604039718178
2403	19	0.061966648915856126
2403	20	0.08835186468181888
2403	21	0.022958389044845262
2403	22	-0.034356416419264235
2403	23	-0.0017239027849598187
2403	24	0.02514112489161846
2403	25	0.0038157243555332343
2403	26	0.010384665967919204
2403	27	0.01780295266691212
2403	28	0.03658945873552
2403	29	0.003934094455253501
2403	30	-0.030636796844490277
2403	31	0.06906260708993983
2403	32	-0.027803425490319
2403	33	0.05965663001518351
2403	34	-0.05216456738218511
2403	35	0.05534981585630305
2403	36	0.12166695369086966
2403	37	0.0013165274494610912
2403	38	0.02777277476172202
2403	39	1.2818879921223711
2403	40	-0.09876022814464847
2403	41	0.008664281235752469
2403	42	-0.015012304078425132
2403	43	-0.00829335252232255
2403	44	0.010654560981429029
2403	45	0.012380397661274856
2403	46	-0.05584446986366487
2403	47	0.011720738227204208
2403	48	-0.09671085512385957
2403	49	-0.02303451094895337
2403	50	-0.007538446829123302
2403	51	-0.0500063523873564
2403	52	-0.03804670037892066
2403	53	-0.06314285916231199
2403	54	-0.005294364958416509
2403	55	-0.21087294827308511
2403	56	-0.13036890522197098
2403	57	-0.03737728166102272
2403	58	-0.07979093910316237
2403	59	-0.022635327857990717
2403	60	-0.028959358779559352
2403	61	0.004861683004421025
2404	1	0.04975386380261426
2404	2	-0.0022503523420027705
2404	3	0.06214389636712525
2404	4	0.01785846493775267
2404	5	0.0966048426234849
2404	6	-0.0692092305408849
2404	7	0.029379554120339435
2404	8	-0.050351474325999845
2404	9	-0.11373923919602902
2404	10	0.01036684936860155
2404	11	0.059059013948946415
2404	12	0.030052009997930895
2404	13	0.02000786362650331
2404	14	0.07504264930496873
2404	15	-0.08897175349327568
2404	16	0.04221153017146406
2404	17	0.010395247021165233
2404	18	-0.02946040418589746
2404	19	0.005688547585094739
2404	20	0.01935770565778938
2404	21	-0.04680951166243119
2404	22	0.03237841688969212
2404	23	0.025071932318304846
2404	24	0.009661261905790752
2404	25	-0.04977052965922013
2404	26	0.007772127174447974
2404	27	0.022608275390702204
2404	28	0.048562724412875014
2404	29	-0.022350297395391294
2404	30	0.09332784006561923
2404	31	0.003056251575756619
2404	32	0.06290579387395923
2404	33	-0.0648925687366173
2404	34	0.003818351496413186
2404	35	0.011452355199132569
2404	36	-0.046411791547718906
2404	37	0.07710452532322165
2404	38	-0.08511033376096577
2404	39	1.30156849870653
2404	40	0.055783453828951224
2404	41	0.05329679649200614
2404	42	0.02667418971887514
2404	43	0.025350483348496766
2404	44	0.0134520240371927
2404	45	-0.023695695190816934
2404	46	0.05600491977020994
2404	47	0.09346461270773432
2404	48	0.05052019420140397
2404	49	0.04448953887691687
2404	50	0.10467692262092498
2404	51	0.050163793408785295
2404	52	5.58219415864869E-5
2404	53	0.0835380598261608
2404	54	0.016214493223245086
2404	55	0.09179584154075826
2404	56	0.042813827030137475
2404	57	0.04520184696656315
2404	58	-0.07100239419403209
2404	59	-0.06596254089460984
2404	60	-0.1203645587879123
2404	61	0.01690768040000279
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	11.0
17	28.0
18	78.0
19	145.0
20	172.0
21	189.0
22	361.0
23	482.0
24	701.0
25	945.0
26	1322.0
27	1601.0
28	2329.0
29	2878.0
30	3636.0
31	4489.0
32	6280.0
33	7435.0
34	9687.0
35	12543.0
36	18804.0
37	28921.0
38	73964.0
39	466316.0
40	414614.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	26.69285615035385	16.96112506392194	23.243292804540182	33.10272598118403
2	26.5049850976595	17.8178472547922	26.906572915563494	28.77059473198481
3	26.803449374297568	20.09923142435565	20.669117362096394	32.428201839250384
4	27.780828806415542	21.309990916231776	19.879935458928795	31.029244818423884
5	27.217275984917734	21.577021563788186	20.110007174380936	31.095695276913144
6	28.448358163245054	24.1890066554435	22.5807732262312	24.781861955080245
7	26.910545205689218	24.289296749977076	22.46091663823066	26.339241406103042
8	24.678641612732775	26.375066048730964	23.170131133315877	25.776161205220376
9	24.877047746970266	26.066350262918846	23.683586169608414	25.37301582050247
10	25.916245955549083	23.968765448786357	23.625359309822663	26.489629285841893
11	24.83498451222244	25.041425196917377	23.611653311983485	26.511936978876694
12	26.632171663369352	23.73595253376638	23.345378857411305	26.286496945452964
13	25.30287892121509	23.096118744984313	23.68717808628351	27.913824247517088
14	25.025261571879454	24.170952547945	23.79578630364362	27.007999576531926
15	25.928439567419804	23.128256946814112	23.094606359015852	27.84869712675023
16	25.621425215822203	24.084084878881516	23.18024521447996	27.11424469081632
17	25.38530395649622	23.153400363539777	23.539814978481584	27.92148070148242
18	26.969055637844058	23.335926445108424	23.126933609091708	26.568084307955814
19	25.585317000825196	23.313335179704538	24.275307179768813	26.826040639701453
20	25.250796129426213	23.364567254386156	23.37770610748716	28.006930508700474
21	25.68863186729569	23.584052268059068	24.15252034395438	26.57479552069086
22	26.72140243550856	23.106610922640513	23.225805841779852	26.946180800071083
23	25.521796790149832	24.25725307227031	23.374397763181154	26.84655237439871
24	25.99517359827815	24.501976026791915	22.994410788605308	26.508439586324627
25	25.588530821008177	23.040727608889426	23.28261483972017	28.08812673038223
26	25.748371113049906	23.065587453246007	24.366806530860707	26.81923490284338
27	26.16843631578997	23.52639255301149	22.814625906604494	27.490545224594044
28	25.750923264371682	23.482911456418236	23.045642863286925	27.720522415923156
29	25.7890164859523	24.728739397938053	23.187996192568324	26.294247923541324
30	27.401503500700898	23.739166353949358	22.835515737793862	26.02381440755588
31	25.771056902576824	23.689824761728318	24.32275828952928	26.216360046165583
32	25.739013224870057	23.59293753562378	23.288002714732812	27.380046524773356
33	26.04753996243611	24.885460393919832	23.01615133690193	26.050848306742125
34	26.05188807209544	23.72262463241932	22.9373182182959	27.288169077189345
35	26.038938267240493	23.90619047934128	22.770577665273066	27.284293588145164
36	26.16456082674579	25.4449486781274	22.612911428060997	25.77757906706581
37	26.0520771203415	24.201389315560277	22.619339068426957	27.12719449567127
38	26.112667083202968	24.330320219371586	23.69351120252644	25.863501494899005
39	26.817007657795145	24.342290668567546	23.412687190818836	25.428014482818472
40	26.311829410424686	24.40074069102805	22.194169563043335	27.09326033550392
41	26.17580919738622	25.677194448409207	22.465359272013014	25.68163708219156
42	26.795603872086176	24.750668994480737	21.907194325527847	26.546532807905244
43	26.455222505059407	25.87285938307886	21.92988011505476	25.742037996806978
44	26.41429355978793	24.99822767269321	23.0189870605928	25.568491706926068
45	26.74304845968215	25.13245192739413	21.52626210972171	26.598237503202004
46	26.286307897206907	25.13386978923956	22.910756939724802	25.669065373828726
47	27.59489985641786	25.004182692444026	21.693664331605746	25.707253119532368
48	28.015343155650037	25.36271269109233	21.251196911707854	25.37074724154978
49	27.69084184129211	25.622275932929462	21.19372624490633	25.4931559808721
50	27.826294909592402	25.377080357792707	21.284469403013997	25.512155329600887
51	27.958723205955778	25.725212702907847	20.906278386775696	25.409785704360683
52	27.772510683589008	25.686741384835116	21.0611089002969	25.479639031278978
53	27.65359933681875	25.94167294464384	20.964221674192363	25.440506044345046
54	27.934903126952516	26.069847655470912	20.499635609505724	25.49561360807085
55	27.83829947321706	26.00906864436339	20.605880723790115	25.546751158629437
56	27.475988509647603	26.201425234727026	20.565424399133782	25.757161856491585
57	27.572970259875174	26.394632542197932	20.296503269116794	25.7358939288101
58	27.4684265798053	26.30077008803032	20.464378111615975	25.76642522054841
59	27.392901805505275	26.412308553204323	20.340362462202165	25.85442717908824
60	27.559925930897194	26.622435678697382	19.868498040042308	25.949140350363116
61	27.545085643581668	26.57224336936908	20.02654237374649	25.856128613302758
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	2.5
2	2.5
3	1.0
4	5.5
5	10.0
6	7.5
7	7.5
8	10.0
9	9.0
10	8.0
11	12.0
12	19.0
13	22.0
14	31.5
15	41.0
16	50.0
17	82.0
18	105.0
19	135.5
20	245.0
21	324.0
22	465.0
23	606.0
24	775.0
25	1278.0
26	1612.0
27	2249.5
28	2887.0
29	3559.5
30	4905.0
31	5578.0
32	6710.5
33	7843.0
34	9518.0
35	13431.5
36	15670.0
37	17905.0
38	20140.0
39	22594.0
40	28291.0
41	31534.0
42	35092.0
43	41894.0
44	45138.0
45	48365.0
46	51592.0
47	54069.0
48	59150.0
49	61754.0
50	63525.5
51	65297.0
52	66585.5
53	69654.5
54	71435.0
55	71017.0
56	70599.0
57	69302.5
58	65025.5
59	62045.0
60	59546.0
61	57519.5
62	57992.0
63	48816.5
64	39641.0
65	35486.5
66	28262.0
67	25192.0
68	22280.0
69	19368.0
70	16612.5
71	11849.5
72	9842.0
73	8166.0
74	6490.0
75	5401.0
76	3450.5
77	2589.0
78	2066.0
79	1543.0
80	1194.5
81	649.0
82	452.0
83	338.5
84	170.5
85	116.0
86	79.5
87	43.0
88	32.0
89	15.0
90	9.0
91	9.0
92	9.0
93	5.5
94	2.5
95	3.0
96	2.5
97	2.0
98	1.5
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03582464262792186
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.066362551054842
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1057931.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.54488192901516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.26460688783854	64.50024385024479
2	11.68784917638708	17.892900704213723
3	2.3502452759193915	5.3969774144842635
4	0.70895605985273	2.170678315771481
5	0.29005147921086377	1.1100978114765883
6	0.1515882924520228	0.6961984768536662
7	0.08976466598084848	0.48097180312310717
8	0.0683208883964106	0.41836914684709364
9	0.042871970881354644	0.29534669542597203
>10	0.32709599613538687	4.959448625195048
>50	0.01636960700172416	0.7965047782154195
>100	0.002145599946968493	0.26708667210561676
>500	0.0	0.0
>1k	0.0	0.0
>5k	1.340999966855308E-4	1.0151757060432551
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTTCACATCTGGGGGGGGGGGGGGG	9890	0.9348435767550057	TruSeq Adapter, Index 7 (97% over 35bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1296577943173989	0.0	0.0	0.0	0.0
2	1.1330606627464361	0.0	0.0	0.0	0.0
3	1.1347620969609549	0.0	0.0	0.0	9.452412302881757E-5
4	1.1360854346833584	0.0	0.0	0.0	9.452412302881757E-5
5	1.137314248282733	0.0	0.0	0.0	9.452412302881757E-5
6	1.1422295026802316	0.0	0.0	0.0	9.452412302881757E-5
7	1.1451597504941249	0.0	0.0	0.0	9.452412302881757E-5
8	1.1758800904784905	0.0	0.0	0.0	1.8904824605763513E-4
9	1.1816460619832485	0.0	9.452412302881757E-5	0.0	1.8904824605763513E-4
10	1.1858996475195451	0.0	9.452412302881757E-5	0.0	1.8904824605763513E-4
11	1.1897751365637268	0.0	9.452412302881757E-5	0.0	1.8904824605763513E-4
12	1.1996056453587238	0.0	9.452412302881757E-5	0.0	1.8904824605763513E-4
13	1.2140678361821329	0.0	9.452412302881757E-5	0.0	1.8904824605763513E-4
14	1.2349576673715015	0.0	9.452412302881757E-5	0.0	1.8904824605763513E-4
15	1.2583996498826484	0.0	9.452412302881757E-5	0.0	1.8904824605763513E-4
16	1.2906323758354752	0.0	9.452412302881757E-5	0.0	1.8904824605763513E-4
17	1.322865101788302	0.0	9.452412302881757E-5	0.0	2.835723690864527E-4
18	1.3615254681070883	0.0	9.452412302881757E-5	0.0	2.835723690864527E-4
19	1.4172947006940906	0.0	9.452412302881757E-5	0.0	2.835723690864527E-4
20	1.487998744719646	0.0	9.452412302881757E-5	0.0	2.835723690864527E-4
21	1.667972674966515	0.0	9.452412302881757E-5	0.0	2.835723690864527E-4
22	1.7787549471562891	0.0	9.452412302881757E-5	0.0	2.835723690864527E-4
23	1.9120339606269219	0.0	9.452412302881757E-5	0.0	2.835723690864527E-4
24	2.05845182719856	0.0	9.452412302881757E-5	0.0	4.7262061514408783E-4
25	2.237858612707256	0.0	9.452412302881757E-5	0.0	5.671447381729054E-4
26	2.4423142908185884	0.0	9.452412302881757E-5	0.0	5.671447381729054E-4
27	2.681460322081497	0.0	9.452412302881757E-5	0.0	5.671447381729054E-4
28	2.94820739726882	0.0	9.452412302881757E-5	0.0	5.671447381729054E-4
29	3.2566396107118516	0.0	9.452412302881757E-5	0.0	5.671447381729054E-4
30	3.5945633505398744	0.0	9.452412302881757E-5	0.0	5.671447381729054E-4
31	3.9716200773018278	0.0	9.452412302881757E-5	0.0	5.671447381729054E-4
32	4.386770025644394	0.0	9.452412302881757E-5	0.0	5.671447381729054E-4
33	4.833774603447673	0.0	9.452412302881757E-5	0.0	5.671447381729054E-4
34	5.3052609291154145	0.0	9.452412302881757E-5	0.0	5.671447381729054E-4
35	5.791209445606566	9.452412302881757E-5	9.452412302881757E-5	0.0	5.671447381729054E-4
36	6.343608420586976	9.452412302881757E-5	9.452412302881757E-5	0.0	5.671447381729054E-4
37	6.92285224650757	9.452412302881757E-5	9.452412302881757E-5	0.0	6.61668861201723E-4
38	7.563631276519924	9.452412302881757E-5	9.452412302881757E-5	0.0	6.61668861201723E-4
39	8.252901181646061	9.452412302881757E-5	1.8904824605763513E-4	0.0	6.61668861201723E-4
40	8.990189341270838	9.452412302881757E-5	2.835723690864527E-4	0.0	9.452412302881757E-4
41	9.74071087811965	9.452412302881757E-5	2.835723690864527E-4	0.0	9.452412302881757E-4
42	10.564772182684882	9.452412302881757E-5	2.835723690864527E-4	0.0	9.452412302881757E-4
43	11.40263400921232	9.452412302881757E-5	3.7809649211527027E-4	0.0	9.452412302881757E-4
44	12.311483452134402	9.452412302881757E-5	3.7809649211527027E-4	0.0	9.452412302881757E-4
45	13.215039544166869	9.452412302881757E-5	5.671447381729054E-4	0.0	9.452412302881757E-4
46	14.161982208669563	9.452412302881757E-5	5.671447381729054E-4	0.0	9.452412302881757E-4
47	15.150988107920082	9.452412302881757E-5	5.671447381729054E-4	0.0	9.452412302881757E-4
48	16.149824515965598	9.452412302881757E-5	5.671447381729054E-4	0.0	9.452412302881757E-4
49	17.21114136933316	9.452412302881757E-5	5.671447381729054E-4	0.0	9.452412302881757E-4
50	18.30015379074817	9.452412302881757E-5	5.671447381729054E-4	0.0	9.452412302881757E-4
>>END_MODULE