##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-26B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 487030 Sequences flagged as poor quality 0 Sequence length 31-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.59958934767879 40.0 40.0 40.0 40.0 40.0 2 39.33859926493235 40.0 40.0 40.0 40.0 40.0 3 39.29559164733179 40.0 40.0 40.0 40.0 40.0 4 39.184970125043634 40.0 40.0 40.0 40.0 40.0 5 39.23431410796049 40.0 40.0 40.0 40.0 40.0 6 39.327704658850585 40.0 40.0 40.0 40.0 40.0 7 39.37499538016139 40.0 40.0 40.0 40.0 40.0 8 39.37840379442745 40.0 40.0 40.0 40.0 40.0 9 39.39917458883436 40.0 40.0 40.0 40.0 40.0 10-11 39.400024639139275 40.0 40.0 40.0 40.0 40.0 12-13 39.32379114222943 40.0 40.0 40.0 40.0 40.0 14-15 39.354684516354226 40.0 40.0 40.0 40.0 40.0 16-17 39.46386875551814 40.0 40.0 40.0 40.0 40.0 18-19 39.49658542594912 40.0 40.0 40.0 40.0 40.0 20-21 39.48105866168409 40.0 40.0 40.0 40.0 40.0 22-23 39.522193704699916 40.0 40.0 40.0 40.0 40.0 24-25 39.49677021949367 40.0 40.0 40.0 40.0 40.0 26-27 39.47142886475166 40.0 40.0 40.0 40.0 40.0 28-29 39.495838038724514 40.0 40.0 40.0 40.0 40.0 30-31 39.47553538796378 40.0 40.0 40.0 40.0 40.0 32-33 39.4755717300998 40.0 40.0 40.0 40.0 40.0 34-35 39.481534423798465 40.0 40.0 40.0 40.0 40.0 36-37 39.45971405319794 40.0 40.0 40.0 40.0 40.0 38-39 39.399035076494656 40.0 40.0 40.0 40.0 40.0 40-41 39.44779445442971 40.0 40.0 40.0 40.0 40.0 42-43 39.434544942564656 40.0 40.0 40.0 40.0 40.0 44-45 39.33558466590301 40.0 40.0 40.0 40.0 40.0 46-47 39.3230102769472 40.0 40.0 40.0 40.0 40.0 48-49 39.40221410710673 40.0 40.0 40.0 40.0 40.0 50-51 39.36298900715941 40.0 40.0 40.0 40.0 40.0 52-53 39.31120623822931 40.0 40.0 40.0 40.0 40.0 54-55 39.222174437559175 40.0 40.0 40.0 40.0 40.0 56-57 39.248078646178115 40.0 40.0 40.0 40.0 40.0 58-59 39.195094624815084 40.0 40.0 40.0 40.0 40.0 60-61 39.116903856569756 40.0 40.0 40.0 40.0 40.0 62-63 39.40370938904226 40.0 40.0 40.0 40.0 40.0 64-65 39.40731044613221 40.0 40.0 40.0 40.0 40.0 66-67 39.405147010398764 40.0 40.0 40.0 40.0 40.0 68-69 39.411065056689665 40.0 40.0 40.0 40.0 40.0 70-71 39.397534521755745 40.0 40.0 40.0 40.0 40.0 72-73 39.401963148722274 40.0 40.0 40.0 40.0 40.0 74-75 39.407763540979175 40.0 40.0 40.0 40.0 40.0 76-77 39.383363506158744 40.0 40.0 40.0 40.0 40.0 78-79 39.391693519127536 40.0 40.0 40.0 40.0 40.0 80-81 39.390659217651454 40.0 40.0 40.0 40.0 40.0 82-83 39.385498550608744 40.0 40.0 40.0 40.0 40.0 84-85 39.37482477157229 40.0 40.0 40.0 40.0 40.0 86-87 39.36075612958517 40.0 40.0 40.0 40.0 40.0 88-89 39.406793100690976 40.0 40.0 40.0 40.0 40.0 90-91 39.475773137493604 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.019224361449559524 1101 2 -0.08014886124256293 1101 3 -0.006219960347415565 1101 4 -0.07099607784012818 1101 5 -0.12469657749835505 1101 6 0.06766300068158415 1101 7 0.11759932675969509 1101 8 -0.02627381585737254 1101 9 -0.1926268658733079 1101 10-11 -0.1004554784281737 1101 12-13 -0.024650895440018417 1101 14-15 -0.0014624673955552225 1101 16-17 0.040867432075295085 1101 18-19 0.026045833299662036 1101 20-21 -0.046440380348308 1101 22-23 0.062163587011610844 1101 24-25 -0.09189637399292394 1101 26-27 -0.09892436207193356 1101 28-29 -0.05477937571970415 1101 30-31 0.13764504336329964 1101 32-33 0.08766245164285635 1101 34-35 -0.02034901590369742 1101 36-37 -0.10101835029129091 1101 38-39 -0.05773103406131952 1101 40-41 -0.15355097218520086 1101 42-43 0.03969961474319916 1101 44-45 -0.0732546850482052 1101 46-47 0.0753317203067283 1101 48-49 -0.0708513243904818 1101 50-51 -0.019780020113515207 1101 52-53 0.030210399894023965 1101 54-55 -0.015858082143097363 1101 56-57 -0.04281558601595492 1101 58-59 0.0016396636870794623 1101 60-61 -0.020617480055605597 1101 62-63 0.11049548680668408 1101 64-65 -0.014319259092467007 1101 66-67 -0.034917390040014595 1101 68-69 -0.07288196010926384 1101 70-71 0.010791217655182095 1101 72-73 0.08217637524863619 1101 74-75 -0.34574521746856135 1101 76-77 -0.13334032703959053 1101 78-79 -0.14125559173808 1101 80-81 -0.04411405579853067 1101 82-83 -0.117185470271572 1101 84-85 0.18315247563571546 1101 86-87 0.25491386322220677 1101 88-89 0.1624735335868479 1101 90-91 -0.5250525459622608 1102 1 0.09385006828978959 1102 2 0.019544180639123 1102 3 0.17099340233666993 1102 4 0.11042524650864038 1102 5 0.01320383772073086 1102 6 -0.09712331513205896 1102 7 -0.17199110778233262 1102 8 -0.27097203567524986 1102 9 -0.10375445306439701 1102 10-11 0.00304966178071453 1102 12-13 -0.14993289339071225 1102 14-15 -0.1820548845349066 1102 16-17 -0.1440947914081434 1102 18-19 -0.19878923275593507 1102 20-21 -0.09383353278740714 1102 22-23 -0.1624265249346024 1102 24-25 -0.12235269944071092 1102 26-27 -0.12107521224859141 1102 28-29 -0.18799832423231067 1102 30-31 -0.11591635974257741 1102 32-33 -0.11905352177485895 1102 34-35 -0.07985936935328652 1102 36-37 -0.15108788657231997 1102 38-39 -0.11104707337278796 1102 40-41 -0.23836853078552878 1102 42-43 -0.15907948075744116 1102 44-45 0.03154695745914182 1102 46-47 -0.18149721515018769 1102 48-49 -0.09393840663902608 1102 50-51 -0.21361717763352317 1102 52-53 -0.02671187135035069 1102 54-55 -0.10998669645223913 1102 56-57 -0.25564230937619925 1102 58-59 -0.14385844363624045 1102 60-61 -0.1201215409722991 1102 62-63 -0.028894584434191017 1102 64-65 -0.020613957273347694 1102 66-67 0.10780132754388205 1102 68-69 0.15382392777602405 1102 70-71 -0.09681718141472828 1102 72-73 0.06860741090710576 1102 74-75 -0.021833614238325083 1102 76-77 -0.0588514868788792 1102 78-79 -0.11950522053616908 1102 80-81 -0.31749999554027397 1102 82-83 -0.13278656037679326 1102 84-85 -0.6924065149783587 1102 86-87 -0.1396717677670125 1102 88-89 -0.0590304837463691 1102 90-91 0.40449290858320097 1103 1 0.15294004906029812 1103 2 0.11459994617191427 1103 3 -0.03685700546122206 1103 4 -0.13189290215524352 1103 5 -0.0340524450191424 1103 6 -0.1152717137793644 1103 7 -0.19583886323621158 1103 8 0.05748575890070384 1103 9 0.014697108503142431 1103 10-11 -0.16103282611796743 1103 12-13 -0.09223274088093092 1103 14-15 0.025480424794714907 1103 16-17 0.003345829240352316 1103 18-19 -0.0803758649421269 1103 20-21 -0.0425881246579749 1103 22-23 -0.012144713383648309 1103 24-25 0.05403029903419565 1103 26-27 -0.07988006603812181 1103 28-29 0.029341109492236228 1103 30-31 0.021223617712493592 1103 32-33 -0.014026555600807455 1103 34-35 -0.07742018958976615 1103 36-37 0.052585945289116864 1103 38-39 0.029136830238407185 1103 40-41 0.03791009469470197 1103 42-43 0.03181712349378785 1103 44-45 0.09234907014487703 1103 46-47 -0.013906534038966356 1103 48-49 -0.02614556625974984 1103 50-51 -0.0700864781086068 1103 52-53 0.01464419947509299 1103 54-55 -0.02522619380285107 1103 56-57 0.03249321860720045 1103 58-59 0.05157678899578855 1103 60-61 0.17881618314107328 1103 62-63 -0.048765835019978 1103 64-65 -0.08397098177476181 1103 66-67 -0.10235144182769318 1103 68-69 0.006655715933959527 1103 70-71 -0.09960424536602375 1103 72-73 -0.007966363541015653 1103 74-75 0.1373486853604291 1103 76-77 0.11293422606728143 1103 78-79 -0.29709833571701694 1103 80-81 -8.352280219270369E-4 1103 82-83 -0.5192378681543062 1103 84-85 0.03489536631334289 1103 86-87 -0.5099526496758031 1103 88-89 0.07809022683511557 1103 90-91 0.3337312378215245 1104 1 0.046002609462334476 1104 2 0.17334770726978377 1104 3 0.1781208954472291 1104 4 0.016963485964076597 1104 5 -0.04475072476915898 1104 6 0.14841851236585768 1104 7 0.10589221079251843 1104 8 0.05524672791043628 1104 9 -0.006866450545885527 1104 10-11 0.06106935801965108 1104 12-13 0.07358028663018246 1104 14-15 0.048710886749489646 1104 16-17 0.021254984578284564 1104 18-19 -0.0016355775895107172 1104 20-21 0.04126523530594994 1104 22-23 0.006513878201950263 1104 24-25 -0.05200101338124341 1104 26-27 0.05641115231120608 1104 28-29 0.04192174218343325 1104 30-31 -0.07787618255810713 1104 32-33 -8.544112183273E-4 1104 34-35 -0.03454580186366485 1104 36-37 0.03176331811614119 1104 38-39 -0.08165820316488492 1104 40-41 -0.015742227408658493 1104 42-43 0.00901551823554314 1104 44-45 0.025745549699323078 1104 46-47 -0.01803249457543643 1104 48-49 -0.007536753489119974 1104 50-51 0.07364743385561212 1104 52-53 -0.013205202522705406 1104 54-55 0.1083025767579926 1104 56-57 0.04089068280546826 1104 58-59 0.09744311529983918 1104 60-61 -0.09842942694554324 1104 62-63 -0.044364349432825634 1104 64-65 0.08289308297850795 1104 66-67 0.0035516837830016357 1104 68-69 0.048153022426213 1104 70-71 -0.010749729958448029 1104 72-73 0.05351297501244545 1104 74-75 -0.02095542810967288 1104 76-77 0.1273267548485464 1104 78-79 0.016009730990241167 1104 80-81 0.00867629110946666 1104 82-83 0.12841089010950668 1104 84-85 0.4549158788647034 1104 86-87 -0.21029267662132867 1104 88-89 -0.4565258187287924 1104 90-91 0.09402040236600584 1201 1 0.05796078271261251 1201 2 0.017068845693444246 1201 3 -0.0803912957323547 1201 4 0.010754625755438951 1201 5 0.0025408564162603398 1201 6 0.08331201827805046 1201 7 0.03525523564735522 1201 8 -0.13553497475160725 1201 9 0.016075098933761467 1201 10-11 0.055133043100532575 1201 12-13 -0.14918967867997424 1201 14-15 -0.002844934056959403 1201 16-17 0.02633785475869388 1201 18-19 0.010367356748460566 1201 20-21 -0.15331220759258457 1201 22-23 -0.018141523590983866 1201 24-25 0.11211004385078382 1201 26-27 0.040107174791224054 1201 28-29 -0.02353289369755629 1201 30-31 -0.039663144647953175 1201 32-33 -0.12634952682161327 1201 34-35 -0.034192409966195214 1201 36-37 -6.927204484838967E-4 1201 38-39 0.14026510237263778 1201 40-41 -0.03201388787587689 1201 42-43 0.051767361497397246 1201 44-45 -0.05459343448175957 1201 46-47 0.08776337625047148 1201 48-49 0.005209713371819191 1201 50-51 2.481739899522495E-4 1201 52-53 -0.08778546333208936 1201 54-55 0.005875286607476937 1201 56-57 0.0643679857991799 1201 58-59 0.0489783893304363 1201 60-61 -0.030892400732064118 1201 62-63 -0.08648312597502184 1201 64-65 0.015190332155313513 1201 66-67 0.049697944018106455 1201 68-69 0.054119699384749254 1201 70-71 -0.013341119574860727 1201 72-73 -0.14956800660749536 1201 74-75 -0.10457876072237582 1201 76-77 -0.14389447489861595 1201 78-79 0.11174501360295608 1201 80-81 -0.026623780336116454 1201 82-83 0.20487023249897618 1201 84-85 0.031195633690693114 1201 86-87 0.019595938705329274 1201 88-89 0.05314293609086462 1201 90-91 0.2536511577414444 1202 1 0.09397422056255067 1202 2 -0.03695131108147365 1202 3 0.1380185586909377 1202 4 0.13554745890213837 1202 5 0.09651622035803342 1202 6 0.07713060730380761 1202 7 0.017576997498792934 1202 8 0.10873527534171501 1202 9 -0.004469880536184689 1202 10-11 0.015297283488735047 1202 12-13 0.023501624044925506 1202 14-15 -0.04108308261985627 1202 16-17 0.07762564871165978 1202 18-19 -0.10270468505818542 1202 20-21 0.0026265755354515363 1202 22-23 0.05155612478527871 1202 24-25 0.022778203611892422 1202 26-27 0.03337331887482975 1202 28-29 0.03165187673917558 1202 30-31 0.05219202519153754 1202 32-33 -0.004818279739879472 1202 34-35 -0.043417986283010634 1202 36-37 -0.09064359510609421 1202 38-39 -0.19325014222211223 1202 40-41 -0.19531833382588104 1202 42-43 -0.21031074573095054 1202 44-45 -0.2149764293592611 1202 46-47 -0.07523060418699146 1202 48-49 -0.0903538542847997 1202 50-51 0.020505092858272178 1202 52-53 -0.1508815306042237 1202 54-55 -0.14036817646510258 1202 56-57 -0.15317267057144335 1202 58-59 -0.08681632663964933 1202 60-61 -0.20675032994468978 1202 62-63 -0.05616116567563978 1202 64-65 0.014229956259022458 1202 66-67 -0.25019368701717326 1202 68-69 -0.013914311929845269 1202 70-71 -0.2439016680706132 1202 72-73 -0.1563034497837421 1202 74-75 -0.25868619808142057 1202 76-77 -0.27282689185165054 1202 78-79 -0.08794907440781685 1202 80-81 -0.2398457379450747 1202 82-83 -0.14676412282269524 1202 84-85 0.1364749772898648 1202 86-87 0.1329083931852395 1202 88-89 0.20093603182377962 1202 90-91 -0.37934547525518525 1203 1 0.0662519526263452 1203 2 0.012643140546600762 1203 3 0.11191848424244455 1203 4 -0.26378642299903987 1203 5 0.103110789536359 1203 6 -0.07044197997725377 1203 7 0.019845717376540506 1203 8 0.11153297135675899 1203 9 0.017284752902938294 1203 10-11 -0.013565221898979019 1203 12-13 -0.08346126925863473 1203 14-15 -0.024112744466961544 1203 16-17 0.11734783828103446 1203 18-19 -0.061873604771363944 1203 20-21 -0.029329460670027174 1203 22-23 -0.13150684296677184 1203 24-25 0.09217595448372151 1203 26-27 0.036993866659010166 1203 28-29 0.06110605173231676 1203 30-31 -0.03482259022322154 1203 32-33 0.015756526486072175 1203 34-35 -0.03832792433434662 1203 36-37 -0.0810621504704443 1203 38-39 -0.08970000572649894 1203 40-41 -0.10443340233322829 1203 42-43 -0.01574431491146555 1203 44-45 -0.08170411927669363 1203 46-47 -0.06451375349210053 1203 48-49 0.040038102873531045 1203 50-51 0.05960077457545765 1203 52-53 -0.05682591443246565 1203 54-55 -0.059692463552046604 1203 56-57 0.0358371874447414 1203 58-59 -0.019765860992052353 1203 60-61 -0.03307736554534557 1203 62-63 -0.09175954964898381 1203 64-65 0.019547512684262358 1203 66-67 -0.01806933169453373 1203 68-69 0.11522467664661207 1203 70-71 0.08869503883296659 1203 72-73 -0.15732995383454806 1203 74-75 0.19726341240171053 1203 76-77 -0.09716420702868334 1203 78-79 0.020533441060145208 1203 80-81 0.08379052057331648 1203 82-83 0.057854109901583683 1203 84-85 0.05807764997949505 1203 86-87 0.15311761339446406 1203 88-89 0.18087475293424404 1203 90-91 0.3990040578113252 1204 1 -0.027001658087520752 1204 2 -0.17942920025417664 1204 3 -0.2269250137887724 1204 4 -0.23015799584018737 1204 5 -0.31927659491505267 1204 6 -0.1656145312672166 1204 7 -0.06639542118208652 1204 8 -0.0797744770465556 1204 9 -0.10497534118457708 1204 10-11 -0.1095352549868096 1204 12-13 -0.15520012464220656 1204 14-15 -0.1828748840186094 1204 16-17 -0.07345639143972704 1204 18-19 -0.10071729450493194 1204 20-21 0.006855317396151861 1204 22-23 -0.12762007771397066 1204 24-25 -0.0486428446160474 1204 26-27 -0.06537902509433735 1204 28-29 0.017227299637966098 1204 30-31 -0.019503655014780463 1204 32-33 -0.11774885332981455 1204 34-35 -0.0799294137696478 1204 36-37 -0.1008110553047814 1204 38-39 -0.13318494406986758 1204 40-41 -0.05987524322695492 1204 42-43 -0.020216017851453216 1204 44-45 -0.08679079185927208 1204 46-47 -0.10572632494292122 1204 48-49 -0.11734859964323618 1204 50-51 0.029697152784891045 1204 52-53 -0.025666937290836245 1204 54-55 -0.017316966468953865 1204 56-57 -0.1270882404964837 1204 58-59 -0.188524216300344 1204 60-61 0.045609803327316456 1204 62-63 -0.1449058790003832 1204 64-65 -0.002951514493034324 1204 66-67 -0.08182185141556886 1204 68-69 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0.027527152316338288 2401 36-37 0.16703023303917774 2401 38-39 0.06765365821613756 2401 40-41 0.07048796406332514 2401 42-43 0.19854241328945932 2401 44-45 0.16021686698497462 2401 46-47 0.1636172065842061 2401 48-49 0.015838655547284475 2401 50-51 0.15321671689467564 2401 52-53 0.11912737353409142 2401 54-55 0.18110352119888518 2401 56-57 0.00350856678896605 2401 58-59 0.1254897787248126 2401 60-61 0.13100483164604526 2401 62-63 0.14447356002318656 2401 64-65 0.21346526818398104 2401 66-67 0.16522208277449124 2401 68-69 0.1151931844608427 2401 70-71 0.13306830695953664 2401 72-73 0.2546611208336458 2401 74-75 0.19039902602916925 2401 76-77 0.33781388751376795 2401 78-79 0.17062978059110634 2401 80-81 0.20097137049206282 2401 82-83 0.27392764303753836 2401 84-85 -0.1442737634865452 2401 86-87 0.003494687300950261 2401 88-89 0.026885703727941745 2401 90-91 -0.10892194167376346 2402 1 -0.10654949825605087 2402 2 -0.048161051753510264 2402 3 0.04365621332701153 2402 4 0.02011518338122187 2402 5 0.02456173658626426 2402 6 0.135400343244946 2402 7 0.0639298478654382 2402 8 0.041895142378550077 2402 9 0.14117872986597746 2402 10-11 0.07424096504114175 2402 12-13 0.11340032470766204 2402 14-15 0.18775714164135593 2402 16-17 0.052954425442273134 2402 18-19 -0.02315835337236649 2402 20-21 -0.02240237009656454 2402 22-23 0.0861796655726863 2402 24-25 0.054066045616465885 2402 26-27 0.02600556617635874 2402 28-29 0.060173387804908884 2402 30-31 -0.02290809142096606 2402 32-33 0.06500353718014651 2402 34-35 0.10841383124193982 2402 36-37 0.04417936860109961 2402 38-39 -0.29559906076291753 2402 40-41 -0.002869189906235192 2402 42-43 0.15488586001906413 2402 44-45 0.0016945160369914447 2402 46-47 -0.06953172973008748 2402 48-49 0.06640496244106942 2402 50-51 -0.0746440538452191 2402 52-53 0.046727237429585955 2402 54-55 0.04717605311542172 2402 56-57 0.03288557265318559 2402 58-59 -0.09663409049372262 2402 60-61 -0.034492953815380645 2402 62-63 0.04683454569872936 2402 64-65 -0.03825005709531837 2402 66-67 0.030133892906690107 2402 68-69 0.09343109976010311 2402 70-71 -0.36832264906387735 2402 72-73 -0.10724746526886975 2402 74-75 -0.04868452315046312 2402 76-77 -0.1500986882095816 2402 78-79 -0.029209510552533402 2402 80-81 0.12944973290086637 2402 82-83 -0.06573383125819987 2402 84-85 -0.02342418152233705 2402 86-87 -0.147103071578492 2402 88-89 -0.011085476449494536 2402 90-91 0.11358381767410464 2403 1 0.013874095406109177 2403 2 0.14363716961744188 2403 3 0.18493013934322988 2403 4 -0.07686511563957055 2403 5 0.18523496192224087 2403 6 0.07612935573935431 2403 7 0.03980085575298631 2403 8 0.008943869417642247 2403 9 0.048802592407930945 2403 10-11 0.006845542341714861 2403 12-13 0.044356123541845704 2403 14-15 0.09304045415404971 2403 16-17 0.010360194344677609 2403 18-19 0.03637112048305369 2403 20-21 0.04080665357417246 2403 22-23 0.022897752800687954 2403 24-25 0.019106947875556557 2403 26-27 0.024185363342176913 2403 28-29 0.02300871985188735 2403 30-31 0.06920348559547307 2403 32-33 0.03287915326109925 2403 34-35 0.052927613605668 2403 36-37 -0.012433824642506863 2403 38-39 -0.005103980084399495 2403 40-41 0.08549450099996392 2403 42-43 0.07984307162817572 2403 44-45 0.1270730729175682 2403 46-47 0.13383237437388118 2403 48-49 0.11261476713896457 2403 50-51 0.0952926668359595 2403 52-53 0.07525948245109504 2403 54-55 -0.03978222643965523 2403 56-57 0.024604137033804818 2403 58-59 0.06691898674857555 2403 60-61 0.0722161553328533 2403 62-63 -0.022676395766282553 2403 64-65 -0.0035414176125740937 2403 66-67 0.15106372645243482 2403 68-69 -0.07826497403613786 2403 70-71 -0.06524623332065005 2403 72-73 0.05012308737061488 2403 74-75 0.011621092652745801 2403 76-77 0.04230336986338301 2403 78-79 0.10219540316493436 2403 80-81 -0.06105923408243541 2403 82-83 -0.04885173287448907 2403 84-85 0.07462261748106158 2403 86-87 0.153915176966386 2403 88-89 0.07032592614628186 2403 90-91 -0.06543716134687116 2404 1 -0.023573938899431823 2404 2 -0.07520892720290107 2404 3 -0.029949331054382355 2404 4 0.07455218308783884 2404 5 0.04765464255105201 2404 6 -0.10803379199052188 2404 7 0.029341285496521152 2404 8 -0.07812935720188818 2404 9 0.1109091791258976 2404 10-11 0.05434107988699566 2404 12-13 0.033409389943166445 2404 14-15 0.023817046436846567 2404 16-17 -0.001169979936555876 2404 18-19 0.018326821353213063 2404 20-21 -0.013561160525831895 2404 22-23 -0.03727751046969274 2404 24-25 -0.008226189052010113 2404 26-27 0.01109742169395389 2404 28-29 -0.03738990684813359 2404 30-31 0.03958472460782758 2404 32-33 0.00672474864910555 2404 34-35 0.07090097928769268 2404 36-37 0.033586955551122344 2404 38-39 0.12766377430351383 2404 40-41 0.12797018075912092 2404 42-43 8.864743494569893E-4 2404 44-45 0.08074826187750261 2404 46-47 -0.028453125539215307 2404 48-49 0.025334174407177557 2404 50-51 0.05416121132451934 2404 52-53 0.07604337896142965 2404 54-55 0.11690178012032248 2404 56-57 -0.04032425127196859 2404 58-59 -0.004953804687104935 2404 60-61 0.11664221235061945 2404 62-63 0.06561616314160545 2404 64-65 -0.019790303803169706 2404 66-67 0.009599952297655534 2404 68-69 -1.3599804801600612E-4 2404 70-71 0.17992313342338662 2404 72-73 0.07335090492143337 2404 74-75 0.0261513752804845 2404 76-77 0.1894350678637693 2404 78-79 0.22477647120114597 2404 80-81 0.19498606872691937 2404 82-83 0.2657061876584166 2404 84-85 0.13377774867181813 2404 86-87 0.07693109008563681 2404 88-89 0.08367212591016226 2404 90-91 -0.2464161823258948 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 16.0 31 294.0 32 2103.0 33 3572.0 34 4845.0 35 5932.0 36 8611.0 37 12823.0 38 31751.0 39 219860.0 40 197223.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 27.330349259799192 15.134796624437918 24.29747654148615 33.237377574276735 2 26.51553582100894 17.7621188440503 26.99940645749599 28.72293887744477 3 26.890951276102086 19.374987167114963 21.138738886721555 32.59532267006139 4 27.515553456665913 21.381434408557993 19.498593515799847 31.604418618976243 5 27.059934706280924 21.774223353797506 20.515779315442582 30.650062624478984 6 27.183335728805208 24.407942015892246 23.3098577089707 25.09886454633185 7 25.71053117877749 24.364618196004354 23.124858838264583 26.799991786953576 8 24.69991581627415 25.365583228959203 23.598340964622302 26.336159990144342 9 24.483707369155905 25.263536127137957 24.321705028437673 25.93105147526846 10-11 24.59930599757715 23.9638215305012 24.232593474734614 27.204278997187032 12-13 25.117549226947006 23.50399359382379 24.077469560396693 27.30098761883252 14-15 25.36486458739708 22.989138246103934 24.077880212717904 27.56811695378108 16-17 25.4125002566577 22.958749974334232 23.851302794489047 27.77744697451902 18-19 25.559308461491074 23.145391454325196 23.727593782723858 27.56770630145987 20-21 25.380777364844054 23.13830770178428 23.9234749399421 27.55743999342956 22-23 25.37718415703345 22.819436174362977 23.925117549226947 27.87826211937663 24-25 25.52563497115167 23.024864998049402 23.801203211301154 27.64829681949777 26-27 25.60047635669261 23.06233702236002 23.819066587273884 27.518120033673487 28-29 25.64503213354413 22.961624540582715 23.807260333039032 27.586082992834115 30-31 25.801695994086604 23.037800546167585 23.79411945876024 27.366384000985565 32-33 25.70826336666134 23.050394684677766 23.918638706289425 27.32270324237147 34-35 25.696864111498257 22.883359627439233 23.896069382693135 27.52370687836937 36-37 25.873071645547043 23.16851322353922 23.82708448172301 27.131330649190726 38-39 26.063135408837372 23.11833273995543 23.745555159970287 27.072976691236907 40-41 25.82777356213396 22.992684294312486 23.898309664432247 27.281232479121304 42-43 25.99267899961773 23.17733733362679 23.779466447345555 27.05051721940992 44-45 26.080203551874998 23.272636836145765 23.75712473411156 26.89003487786768 46-47 25.95535427992032 23.07879916358914 23.766894422318156 27.198952134172384 48-49 26.130380404852467 23.309640594360065 23.689993978166996 26.869985022620472 50-51 26.293467560740098 23.252937455597383 23.68618613577364 26.767408847888884 52-53 26.154937087849085 23.160929324251885 23.82754627838363 26.8565873095154 54-55 26.469795517012606 23.321101080466306 23.60573049947872 26.603372903042366 56-57 26.381433508611906 23.288530648196968 23.562947746541234 26.76708809664989 58-59 26.41853067499431 23.347959842677863 23.489244285949088 26.744265196378734 60-61 26.740616814413364 23.36004707099994 23.493497609232723 26.405838505353973 62-63 26.654774628120233 23.44791781561762 23.26668466953952 26.630622886722627 64-65 26.620322832381817 23.346613545816734 23.381320080502732 26.65174354129872 66-67 26.684264994155 23.471191865728187 23.24856780757771 26.5959753325391 68-69 27.03216850331625 23.38710664645985 23.0653971300769 26.515327720147 70-71 26.94860205158741 23.401629360976532 23.066284136736407 26.58348445069965 72-73 27.235330361349714 23.423700965290614 22.780979304845783 26.559989368513886 74-75 27.23715497272532 23.428934018810832 22.81794022426986 26.51597078419399 76-77 27.21973813407817 23.375465033036416 22.75072664468151 26.654070188203903 78-79 27.681119059902027 23.463187257180163 22.241041631475387 26.614652051442423 80-81 28.034268099405807 23.16745728741742 22.106518672628546 26.691755940548223 82-83 28.20378679651502 23.133932942983428 21.793865738411906 26.868414522089644 84-85 29.16818331513355 22.53566290537847 21.034614251295654 27.26153952819233 86-87 29.836322060416414 21.77157299313997 20.349019135876762 28.043085810566854 88-89 29.198251856435643 21.70676051980198 20.295095915841586 28.799891707920793 90-91 26.741345134375145 22.09929470336053 20.485871018300834 30.67348914396349 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 1.0 10 2.5 11 6.0 12 10.0 13 12.5 14 19.5 15 27.0 16 39.0 17 51.5 18 71.0 19 115.0 20 177.5 21 255.0 22 344.5 23 456.5 24 604.5 25 797.5 26 1021.0 27 1308.0 28 1693.5 29 2068.5 30 2459.5 31 2996.5 32 3711.0 33 4424.5 34 5200.0 35 6272.0 36 7519.0 37 8822.0 38 10267.0 39 11859.5 40 13470.5 41 15092.0 42 16813.0 43 18740.5 44 20519.0 45 22048.5 46 23601.0 47 25156.0 48 26441.5 49 27367.5 50 28137.0 51 28881.5 52 29446.5 53 29792.0 54 29924.0 55 29662.5 56 29250.5 57 28739.5 58 28235.5 59 27713.0 60 26893.0 61 25916.0 62 24703.0 63 23215.5 64 21604.0 65 19884.0 66 17839.0 67 16000.5 68 14358.0 69 12426.5 70 10529.0 71 8836.5 72 7306.0 73 5858.0 74 4610.0 75 3633.5 76 2834.0 77 2067.5 78 1485.5 79 1093.0 80 775.0 81 552.0 82 399.0 83 275.5 84 188.0 85 124.0 86 74.5 87 43.5 88 28.5 89 22.0 90 16.5 91 12.5 92 9.0 93 6.0 94 4.0 95 2.5 96 2.0 97 1.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.025255117754553108 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.06468028283622491 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.0536368882472414E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 3.020299432485737E-4 74-75 0.0 76-77 3.911934529863708E-4 78-79 0.0 80-81 5.446267128510119E-4 82-83 6.706998081798548E-4 84-85 8.666487559257108E-4 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 3444.0 32-33 7418.0 34-35 8498.0 36-37 9706.0 38-39 10989.0 40-41 12112.0 42-43 13238.0 44-45 14102.0 46-47 14955.0 48-49 16121.0 50-51 16769.0 52-53 17540.0 54-55 18009.0 56-57 18276.0 58-59 18922.0 60-61 19295.0 62-63 19296.0 64-65 19701.0 66-67 19491.0 68-69 19723.0 70-71 19096.0 72-73 19017.0 74-75 18882.0 76-77 18403.0 78-79 17947.0 80-81 17301.0 82-83 16945.0 84-85 16349.0 86-87 15850.0 88-89 15043.0 90-91 14592.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.78175949681302 #Duplication Level Percentage of deduplicated Percentage of total 1 99.79934851042555 99.58154591005906 2 0.1875565210328232 0.37429439347512233 3 0.009497747663382098 0.02843105919327031 4 0.0027375725872506493 0.010926392380244499 5 6.023948893448187E-4 0.0030054010985357 6 0.0 0.0 7 2.572534016575841E-4 0.0017968437937753866 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0014372831242428598 0.0 0.0 0.0 0.0 2 0.0014372831242428598 0.0 0.0 0.0 0.0 3 0.0014372831242428598 0.0 0.0 0.0 0.0 4 0.0014372831242428598 0.0 0.0 0.0 0.0 5 0.0014372831242428598 0.0 0.0 0.0 0.0 6 0.0014372831242428598 0.0 0.0 0.0 0.0 7 0.0016426092848489827 0.0 0.0 0.0 0.0 8 0.006159784818183685 0.0 0.0 0.0 2.0532616060612283E-4 9 0.006570437139395931 0.0 0.0 0.0 2.0532616060612283E-4 10-11 0.006570437139395931 0.0 0.0 0.0 2.0532616060612283E-4 12-13 0.006570437139395931 0.0 0.0 0.0 2.0532616060612283E-4 14-15 0.006570437139395931 0.0 0.0 0.0 2.0532616060612283E-4 16-17 0.006570437139395931 0.0 0.0 0.0 3.0798924090918425E-4 18-19 0.006673100219698992 0.0 0.0 0.0 4.1065232121224567E-4 20-21 0.006775763300002053 0.0 0.0 0.0 4.1065232121224567E-4 22-23 0.006775763300002053 0.0 0.0 0.0 4.1065232121224567E-4 24-25 0.006775763300002053 0.0 0.0 0.0 7.186415621214299E-4 26-27 0.006775763300002053 0.0 0.0 0.0 8.213046424244913E-4 28-29 0.006775763300002053 0.0 0.0 0.0 8.213046424244913E-4 30-31 0.006775763300002053 0.0 0.0 0.0 8.213046424244913E-4 32-33 0.006878426380305115 0.0 0.0 0.0 8.213046424244913E-4 34-35 0.006981089460608176 1.0266308030306142E-4 0.0 0.0 8.213046424244913E-4 36-37 0.007186415621214299 2.0532616060612283E-4 0.0 0.0 8.213046424244913E-4 38-39 0.007597067942426545 2.0532616060612283E-4 0.0 0.0 8.213046424244913E-4 40-41 0.00800772026363879 2.0532616060612283E-4 2.0532616060612283E-4 0.0 0.0010266308030306142 42-43 0.00800772026363879 2.0532616060612283E-4 2.0532616060612283E-4 0.0 0.0010266308030306142 44-45 0.00800772026363879 2.0532616060612283E-4 3.0798924090918425E-4 0.0 0.0010266308030306142 46-47 0.008213046424244913 2.0532616060612283E-4 4.1065232121224567E-4 0.0 0.0010266308030306142 48-49 0.008213046424244913 2.0532616060612283E-4 4.1065232121224567E-4 0.0 0.0010266308030306142 50-51 0.008623698745457159 2.0532616060612283E-4 4.1065232121224567E-4 0.0 0.0010266308030306142 52-53 0.008623698745457159 2.0532616060612283E-4 4.1065232121224567E-4 0.0 0.0010266308030306142 54-55 0.008623698745457159 2.0532616060612283E-4 4.1065232121224567E-4 0.0 0.0010266308030306142 56-57 0.008931687986366343 2.0532616060612283E-4 4.1065232121224567E-4 0.0 0.0010266308030306142 58-59 0.009034351066669404 2.0532616060612283E-4 4.1065232121224567E-4 0.0 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0.01088228651212451 2.0532616060612283E-4 6.159784818183685E-4 0.0 0.0010266308030306142 >>END_MODULE