##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-26A_S16_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1001014
Sequences flagged as poor quality	0
Sequence length	61
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.490400733656074	40.0	40.0	40.0	40.0	40.0
2	39.2304982747494	40.0	40.0	40.0	40.0	40.0
3	39.14741851762313	40.0	40.0	40.0	40.0	40.0
4	39.07054246993548	40.0	40.0	40.0	40.0	40.0
5	39.104024519137596	40.0	40.0	40.0	40.0	40.0
6	39.22155734085637	40.0	40.0	40.0	40.0	40.0
7	39.24047016325446	40.0	40.0	40.0	40.0	40.0
8	39.241720895012456	40.0	40.0	40.0	40.0	40.0
9	39.270891316205365	40.0	40.0	40.0	40.0	40.0
10	39.27394022461225	40.0	40.0	40.0	40.0	40.0
11	39.26504924007057	40.0	40.0	40.0	40.0	40.0
12	39.28030576994927	40.0	40.0	40.0	40.0	40.0
13	39.11303138617442	40.0	40.0	40.0	40.0	40.0
14	39.277980128150055	40.0	40.0	40.0	40.0	40.0
15	39.186612774646505	40.0	40.0	40.0	40.0	40.0
16	39.36813271342859	40.0	40.0	40.0	40.0	40.0
17	39.31971381019646	40.0	40.0	40.0	40.0	40.0
18	39.394378100605984	40.0	40.0	40.0	40.0	40.0
19	39.346071083920904	40.0	40.0	40.0	40.0	40.0
20	39.3022515169618	40.0	40.0	40.0	40.0	40.0
21	39.335641659357414	40.0	40.0	40.0	40.0	40.0
22	39.38871184618797	40.0	40.0	40.0	40.0	40.0
23	39.37900968418024	40.0	40.0	40.0	40.0	40.0
24	39.382446199553655	40.0	40.0	40.0	40.0	40.0
25	39.34934776136997	40.0	40.0	40.0	40.0	40.0
26	39.31091073651318	40.0	40.0	40.0	40.0	40.0
27	39.321711784250766	40.0	40.0	40.0	40.0	40.0
28	39.32853286767218	40.0	40.0	40.0	40.0	40.0
29	39.314958631947206	40.0	40.0	40.0	40.0	40.0
30	39.33082654188653	40.0	40.0	40.0	40.0	40.0
31	39.27024796855988	40.0	40.0	40.0	40.0	40.0
32	39.2904275065084	40.0	40.0	40.0	40.0	40.0
33	39.300161636100995	40.0	40.0	40.0	40.0	40.0
34	39.29273716451518	40.0	40.0	40.0	40.0	40.0
35	39.295734125596645	40.0	40.0	40.0	40.0	40.0
36	39.2699802400366	40.0	40.0	40.0	40.0	40.0
37	39.25750888598961	40.0	40.0	40.0	40.0	40.0
38	39.25087161618119	40.0	40.0	40.0	40.0	40.0
39	37.9988891264258	40.0	40.0	40.0	40.0	40.0
40	39.24351907166133	40.0	40.0	40.0	40.0	40.0
41	39.20363950953733	40.0	40.0	40.0	40.0	40.0
42	39.21770924282777	40.0	40.0	40.0	40.0	40.0
43	39.21913579630255	40.0	40.0	40.0	40.0	40.0
44	39.17453302351416	40.0	40.0	40.0	40.0	40.0
45	39.012900918468674	40.0	40.0	40.0	40.0	40.0
46	39.01397183256178	40.0	40.0	40.0	40.0	40.0
47	39.10730918848288	40.0	40.0	40.0	40.0	40.0
48	39.17413342870329	40.0	40.0	40.0	40.0	40.0
49	39.150301594183496	40.0	40.0	40.0	40.0	40.0
50	39.11506931970981	40.0	40.0	40.0	40.0	40.0
51	39.0762526797827	40.0	40.0	40.0	40.0	40.0
52	39.083673155420406	40.0	40.0	40.0	40.0	40.0
53	39.00110088370393	40.0	40.0	40.0	40.0	40.0
54	38.90059080092786	40.0	40.0	40.0	40.0	40.0
55	38.93409282987051	40.0	40.0	40.0	40.0	40.0
56	38.948965748730785	40.0	40.0	40.0	40.0	40.0
57	38.88003963980524	40.0	40.0	40.0	40.0	40.0
58	38.839320928578424	40.0	40.0	40.0	40.0	40.0
59	38.76539189262088	40.0	40.0	40.0	40.0	40.0
60	38.73186588798958	40.0	40.0	40.0	40.0	40.0
61	38.60758390991535	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.005741772648676147
1101	2	-0.11459971428985938
1101	3	0.02410144288577243
1101	4	0.02262357416697114
1101	5	-0.08057339844668121
1101	6	0.049696451562070365
1101	7	0.04004704580044205
1101	8	0.020385776532357625
1101	9	-0.04227133729579435
1101	10	0.07246066480188773
1101	11	-0.04077941084291581
1101	12	-0.031183550681816996
1101	13	-0.062128493086959224
1101	14	-0.05837511989036415
1101	15	-0.1190757229587831
1101	16	-0.01706441371071321
1101	17	-0.02416655395311551
1101	18	0.01665174784094603
1101	19	-0.1026301873449782
1101	20	0.046594099128803634
1101	21	0.03394068004674011
1101	22	0.03804376306955959
1101	23	-0.004203466341301976
1101	24	0.08507825337356678
1101	25	-0.021304909883198775
1101	26	0.06312157143248243
1101	27	-0.08605452619504916
1101	28	0.08346487254005552
1101	29	0.021039058438056202
1101	30	0.0024633828742111064
1101	31	-0.0802411136518657
1101	32	-0.0380766349651509
1101	33	0.12157733424302108
1101	34	-0.03672923683322438
1101	35	-5.911073192592653E-4
1101	36	-0.1699000775875703
1101	37	-0.0719148638974687
1101	38	0.01952316920792896
1101	39	1.2873769849915817
1101	40	-0.12331174097386821
1101	41	-0.14143181743776978
1101	42	0.029863997127165476
1101	43	-0.05249205126622769
1101	44	-0.08689218840646618
1101	45	0.06107204141327571
1101	46	-0.11371922628141817
1101	47	-0.004502977935729291
1101	48	-0.006987488016328314
1101	49	0.0012195406570540968
1101	50	-0.05355538353312994
1101	51	-0.017270922713130687
1101	52	-0.020929908765047855
1101	53	-0.08273829500733854
1101	54	0.04826895233014028
1101	55	-0.0011047945605326959
1101	56	0.06600848576204754
1101	57	-0.034573706635157464
1101	58	0.006752738219354626
1101	59	-0.11416314555353324
1101	60	-0.03515608249870894
1101	61	-0.07326027688968395
1102	1	0.10617707683538669
1102	2	0.07152903391515508
1102	3	0.13763683371782776
1102	4	0.06448027362011288
1102	5	0.0351260682170107
1102	6	-0.010810654003158504
1102	7	0.027198604995319897
1102	8	0.055110942625475445
1102	9	0.06886126693095207
1102	10	-0.029261874492789275
1102	11	0.0703284991658677
1102	12	0.06114617043618864
1102	13	0.05611918168784058
1102	14	0.010099202728618195
1102	15	-0.09983187167207319
1102	16	-0.042305432217851546
1102	17	-0.03449148086508558
1102	18	-0.10374745967064314
1102	19	-0.016554374919905968
1102	20	-0.07140232177747663
1102	21	-0.006298744115454724
1102	22	-0.017125471697781336
1102	23	0.02326120769796347
1102	24	-0.030147711459903803
1102	25	0.012306664571141823
1102	26	0.05172695371367553
1102	27	-0.028818032609372324
1102	28	-0.0719781740679295
1102	29	0.0459280863462439
1102	30	-0.009002573696498928
1102	31	0.06590514784932111
1102	32	0.0618153964262973
1102	33	0.15165881144329774
1102	34	0.030626006542988193
1102	35	0.05300553693250265
1102	36	0.008645781829024202
1102	37	0.01655824752891988
1102	38	0.015380812136982058
1102	39	1.273264043437898
1102	40	0.0314116455676654
1102	41	-0.026895330848788035
1102	42	0.011908164891842432
1102	43	0.07984896647656115
1102	44	0.042697579877490455
1102	45	0.01011297482335749
1102	46	0.05684452115630734
1102	47	0.03241095820698092
1102	48	-0.008795653531869618
1102	49	-0.021980873108290666
1102	50	-0.044353455581060075
1102	51	0.13233409719020983
1102	52	0.13369195710262716
1102	53	0.08282506927803723
1102	54	0.08609618256590323
1102	55	0.09059659590032965
1102	56	-0.1618019465259195
1102	57	0.010538709872413676
1102	58	-0.05969587458294967
1102	59	-0.08953791460869809
1102	60	-0.07188226419053478
1102	61	0.05386046156934299
1103	1	0.05860314846221115
1103	2	-0.05560270518883925
1103	3	0.07446898564078452
1103	4	0.12983443138536188
1103	5	-0.016008941829404932
1103	6	-0.044380424359296455
1103	7	0.006406582933891514
1103	8	-5.706154029567756E-4
1103	9	0.10271730285398917
1103	10	-0.1126588977182621
1103	11	0.0029817161284384497
1103	12	0.04087554682272554
1103	13	-0.05958905055837249
1103	14	-0.025665399430039315
1103	15	-0.016544861194901728
1103	16	-0.042815984338524515
1103	17	-0.013395761639316106
1103	18	-0.008451240661607073
1103	19	-0.02693624698824948
1103	20	-0.15594828095887436
1103	21	-0.022948243886709463
1103	22	-0.04911361511256729
1103	23	-0.02161082307151929
1103	24	0.02574883627033131
1103	25	0.007669472282415768
1103	26	-0.004965636966460352
1103	27	0.0156414503539537
1103	28	0.0012078067441407825
1103	29	0.09794203627262732
1103	30	2.7103614328893855E-4
1103	31	0.0825081633607283
1103	32	0.09062246699211585
1103	33	-0.07565115606033146
1103	34	0.028635318114979214
1103	35	0.005279372608320898
1103	36	0.030960163400500562
1103	37	0.04144940657133134
1103	38	-0.052069011009777455
1103	39	1.294586373430434
1103	40	0.039258963367764466
1103	41	0.07592359087907852
1103	42	0.12280450150606015
1103	43	-0.06394389346365159
1103	44	0.06612758368532923
1103	45	0.022717569250126246
1103	46	0.02656777324161652
1103	47	0.0504100509891785
1103	48	-0.0516963643149424
1103	49	-0.022035104744780654
1103	50	-0.023880625437257663
1103	51	-0.02595914024092849
1103	52	-0.04322984746282543
1103	53	0.0908498020032269
1103	54	-0.04950847500198563
1103	55	0.15342782046230496
1103	56	-0.10775818391947922
1103	57	0.0444373563669771
1103	58	0.1030544286300028
1103	59	-0.00812421980342748
1103	60	0.07544533634232664
1103	61	0.11098806744789869
1104	1	0.04769791155348457
1104	2	0.05121260041519804
1104	3	-0.057717476365539255
1104	4	0.025308306517992207
1104	5	0.19092997316894866
1104	6	0.15332816699091012
1104	7	0.044877470673725384
1104	8	0.012823518740482598
1104	9	0.1103240348117751
1104	10	-8.435185135198253E-4
1104	11	0.03552874200366318
1104	12	0.09051384910518578
1104	13	0.052859692520677015
1104	14	0.16451523260504786
1104	15	0.09537316115027039
1104	16	-0.033502511789791356
1104	17	0.007919399349290757
1104	18	0.026139895506460675
1104	19	0.10421980519743101
1104	20	-0.021267351935016165
1104	21	-0.09906608935149563
1104	22	0.004382557452032643
1104	23	-0.058494720550910984
1104	24	-0.041062299953431136
1104	25	-0.025711429648424655
1104	26	-0.014343162017553368
1104	27	0.03314131011477883
1104	28	0.1207214214814698
1104	29	0.13448339210894034
1104	30	-0.10813921655378067
1104	31	0.03259614603711469
1104	32	-0.06130768156592836
1104	33	0.004380655356371221
1104	34	-0.10428715090608875
1104	35	-0.03556066203572783
1104	36	-0.060914597645876256
1104	37	-0.13560006463746532
1104	38	-0.0473701035226739
1104	39	1.2444265007715813
1104	40	-0.024698852688565864
1104	41	-0.034796132477765696
1104	42	-0.08113110703050808
1104	43	0.05368832820734326
1104	44	-0.14618656957042475
1104	45	-0.03224464971952301
1104	46	-0.014750467041203308
1104	47	0.04161894450010095
1104	48	-0.0890628436910248
1104	49	0.035199983454724304
1104	50	-0.040126488381609704
1104	51	-0.07453997947192903
1104	52	-0.05756368353536345
1104	53	0.0460241899139362
1104	54	-0.08910246966215851
1104	55	-0.0757591941460447
1104	56	-0.07118500075279854
1104	57	0.07466297633353491
1104	58	-0.11055907538799659
1104	59	-0.08277323397176417
1104	60	-0.1328426596219643
1104	61	0.07780171007392056
1201	1	0.07211718640879639
1201	2	0.08883738882654058
1201	3	0.13211968478120895
1201	4	-0.015465587480726128
1201	5	0.01717770220820114
1201	6	0.07511877508063947
1201	7	0.12259902935092981
1201	8	-0.09957301804374907
1201	9	0.01075683173198172
1201	10	0.044010726300925285
1201	11	0.09686606737892589
1201	12	0.006923884743223141
1201	13	-0.019197558050819907
1201	14	0.012163993477621204
1201	15	0.0754828515932715
1201	16	0.06137324831111357
1201	17	0.07077505063617195
1201	18	0.04008456245172454
1201	19	0.08216085881516477
1201	20	0.0765246023983508
1201	21	0.017762166237503152
1201	22	0.04767365492573816
1201	23	0.04175080683572929
1201	24	0.030992149278745273
1201	25	-0.03470853116266426
1201	26	0.06680043273509995
1201	27	0.05275308650507071
1201	28	0.012251962233001734
1201	29	0.048527096403965686
1201	30	0.11823981526133309
1201	31	0.07994798495129629
1201	32	0.08944439300091744
1201	33	0.09511923747248119
1201	34	0.054585606159058386
1201	35	0.033446330207262065
1201	36	0.10644181545522713
1201	37	0.08706038932557192
1201	38	0.09604286221624392
1201	39	1.208317236932544
1201	40	0.017514929720803707
1201	41	0.004567887460169118
1201	42	0.18727081592885497
1201	43	8.094468141450761E-4
1201	44	0.09513640694464698
1201	45	-0.04649946494667745
1201	46	0.01714318131207193
1201	47	0.003221386627686229
1201	48	0.08616173117821546
1201	49	0.1306488175423013
1201	50	0.11244205463880519
1201	51	0.018147934619797468
1201	52	0.0020476166272445084
1201	53	-0.017812730517718478
1201	54	0.012147137775158967
1201	55	0.2102827625134367
1201	56	0.07808356454827248
1201	57	0.07541693680203565
1201	58	0.1863660845309596
1201	59	0.16484555257008537
1201	60	0.00865899231782663
1201	61	0.07225225688035408
1202	1	0.06537890421284942
1202	2	0.054682786990156274
1202	3	0.09551293058967048
1202	4	0.05054116430386557
1202	5	0.04394375432999453
1202	6	0.08393172963069873
1202	7	0.03784429986799154
1202	8	0.05199547593726095
1202	9	0.10625633669308598
1202	10	0.08574682512045229
1202	11	0.0925652610217611
1202	12	-0.04885039982796968
1202	13	0.09524795973619149
1202	14	-0.035868135177899774
1202	15	0.02578464479939413
1202	16	-0.060385871113560086
1202	17	-0.026319693810947342
1202	18	-0.017525656473956985
1202	19	0.01949878726793486
1202	20	-4.8797116890852976E-4
1202	21	-0.06842381712187517
1202	22	0.050591737753926225
1202	23	0.08107173812135926
1202	24	-0.014090195273219308
1202	25	-0.027045041374726964
1202	26	0.09173955209910645
1202	27	-0.008017084238588268
1202	28	-0.1028921577332369
1202	29	-0.037424062693482085
1202	30	0.051011194567188056
1202	31	0.037813076260263756
1202	32	-0.0984271491262092
1202	33	0.0359525250640047
1202	34	0.07683442222894854
1202	35	-0.0027690502143968843
1202	36	-0.26142682854913346
1202	37	-0.13645591026674708
1202	38	-0.12515885290102347
1202	39	0.9955598022741015
1202	40	-0.23248171914129045
1202	41	-0.2697107642063159
1202	42	-0.17658585556051065
1202	43	-0.10320008939913095
1202	44	-0.1798846807632941
1202	45	-0.20705233518895483
1202	46	-0.0014098922488798848
1202	47	-0.08590772748115683
1202	48	-0.0942741006950456
1202	49	-0.11653228841931451
1202	50	-0.04723179011632794
1202	51	-0.0650616512196791
1202	52	0.10000025237792443
1202	53	-0.07740682308290303
1202	54	0.032568629713495056
1202	55	-0.03829163406893343
1202	56	-0.1759900248746149
1202	57	-0.08875653218535717
1202	58	0.07722407269953635
1202	59	-0.18349354031749243
1202	60	-0.03880094788613064
1202	61	-0.06541424942091112
1203	1	0.04325922872890686
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2403	3	0.03883368260557063
2403	4	-0.0674460818558984
2403	5	0.03219752069556847
2403	6	-0.11503478534437761
2403	7	0.07656282248545665
2403	8	-0.020537516755297247
2403	9	0.12447768161847961
2403	10	0.020342327964783635
2403	11	0.06984731103318609
2403	12	-0.010855004718628436
2403	13	0.01503586680512825
2403	14	1.9647277778744865E-4
2403	15	0.09843509982829346
2403	16	-0.04170201823880859
2403	17	-0.024957864848957456
2403	18	0.10395242349403588
2403	19	-0.008218653411290688
2403	20	-0.01034746602580583
2403	21	0.02557174683958152
2403	22	0.043180598470208054
2403	23	-0.02789660302597241
2403	24	0.02856698630979082
2403	25	-0.032723611197368996
2403	26	-0.014993333959672839
2403	27	0.08208019843544179
2403	28	0.05115969638221429
2403	29	-0.0050742011246853735
2403	30	0.07478263488485482
2403	31	0.04105797330177552
2403	32	0.016616197228927376
2403	33	0.011571748876562538
2403	34	-0.020009603388857045
2403	35	-0.001517342192734361
2403	36	-0.028425601618323526
2403	37	-0.01687925186832473
2403	38	-0.012464753272773521
2403	39	1.2977977350838117
2403	40	0.07638182357783307
2403	41	0.09203285405577333
2403	42	-0.019373530457805543
2403	43	0.03225600859382638
2403	44	0.005514934364022395
2403	45	0.11004458316829613
2403	46	-0.0147859158000756
2403	47	-0.01954517814468204
2403	48	-0.028851250049328314
2403	49	0.07907553787591581
2403	50	-0.026745624518298428
2403	51	-0.011058342085419781
2403	52	-0.09760076293714093
2403	53	0.05140531151058525
2403	54	0.06652258631299901
2403	55	-0.11417230334730277
2403	56	0.14177863121860668
2403	57	-0.012413354957637068
2403	58	0.0393241157384665
2403	59	0.008483965645098124
2403	60	-7.468216086152779E-4
2403	61	-0.007255710138480254
2404	1	-0.10837974855101606
2404	2	-0.02292083184482152
2404	3	-0.09888544669926347
2404	4	0.0555179535250403
2404	5	-0.05111754955641601
2404	6	-0.05270565584031317
2404	7	0.0747770989759644
2404	8	0.0683147721806705
2404	9	0.09049875758330472
2404	10	0.03403484261029632
2404	11	-0.04514147654171552
2404	12	-0.00905452316359856
2404	13	0.08784320863762218
2404	14	0.14397230396936322
2404	15	0.1745364374237539
2404	16	0.017707969858825834
2404	17	-0.045102597001495326
2404	18	0.08469907729168824
2404	19	0.049919191161400533
2404	20	-5.363173554684408E-4
2404	21	0.04219225771884538
2404	22	0.023649801011842442
2404	23	0.01731909687802613
2404	24	0.08969185929836243
2404	25	0.012297282505592477
2404	26	0.07553251733973809
2404	27	0.046817324438897856
2404	28	0.11087665182693485
2404	29	0.02046312086561386
2404	30	0.0630145693768398
2404	31	0.14336959976383667
2404	32	0.008245433802606783
2404	33	-0.10348197409869186
2404	34	0.08740830682762635
2404	35	0.07594468720086667
2404	36	0.11783548444100234
2404	37	-0.06588481786519651
2404	38	0.09593604277620216
2404	39	1.3127919093453357
2404	40	0.11616065950747867
2404	41	0.08625360342504962
2404	42	0.021211094757944693
2404	43	0.07579224291616526
2404	44	0.12277350934190423
2404	45	-0.10230007917868988
2404	46	0.022286294578819366
2404	47	0.03882584354743557
2404	48	0.0675217388902567
2404	49	-0.02602535919188398
2404	50	0.09271485085310616
2404	51	0.00679594140040507
2404	52	0.020109408234048942
2404	53	-0.02067298287258268
2404	54	0.06446236315658638
2404	55	0.04061302981188675
2404	56	0.0842370116854454
2404	57	-0.039677368275292224
2404	58	0.06548127564115447
2404	59	0.10834575654867962
2404	60	-0.06514798070551109
2404	61	0.18936277888854391
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	3.0
17	27.0
18	62.0
19	109.0
20	156.0
21	175.0
22	301.0
23	427.0
24	688.0
25	899.0
26	1221.0
27	1543.0
28	2114.0
29	2560.0
30	3283.0
31	4323.0
32	5813.0
33	6995.0
34	8940.0
35	11546.0
36	17396.0
37	25799.0
38	72124.0
39	495242.0
40	339268.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	26.446283468562875	18.01922850229867	23.800666124549707	31.733821904588748
2	27.454458422730966	17.935821577149117	27.042438184472744	27.567281815647178
3	26.863786983785428	21.578441039227265	20.653178330351356	30.90459364663596
4	27.282335711588452	21.712083946877865	21.22647635297808	29.779103988555605
5	26.663263450860825	21.837157122677606	20.45535826671755	31.04422115974402
6	29.073719248681833	24.17358798178647	22.454131510648203	24.298561258883492
7	27.565348736381313	24.450307388308257	22.262625697542692	25.721718177767745
8	24.435822076414514	27.516997764267032	22.78060047112228	25.266579688196167
9	24.66628838357905	27.13088927827183	23.35941355465558	24.843408783493537
10	26.61121622674608	24.21564533562967	23.28019388340223	25.89294455422202
11	24.593162533191343	26.021614083319516	23.322550933353583	26.062672450135558
12	27.3272901278104	24.165496186866516	22.73964200300895	25.767571682314134
13	25.03990953173482	23.514556239972666	22.953824821630867	28.49170940666165
14	25.17357399596809	25.269276953169484	23.146029925655384	26.411119125207037
15	25.575566375695047	23.511359481485776	22.625257988399763	28.28781615441942
16	25.395449014699096	25.495447616117257	22.574809143528462	26.534294225655188
17	25.186860523429246	23.50546545802556	22.86401588788968	28.443658130655514
18	27.750760728621177	23.730137640432602	22.51257225173674	26.006529379209482
19	25.308736940742087	23.669399229181607	24.654600235361343	26.367263594714956
20	25.158988785371633	23.60576375555187	22.844635539562884	28.390611919513614
21	25.665375309436232	23.833532797743086	24.429228762035297	26.071863130785385
22	27.407608684793622	23.46890253283171	22.648933980943323	26.47455480143135
23	25.303542208200884	25.653387465110377	22.737943725062788	26.30512660162595
24	25.69344684489927	25.99164447250488	22.426259772590594	25.88864891000525
25	25.491851262819505	23.499871130673498	22.63165150537355	28.37662610113345
26	25.473270104114427	23.591877835874424	24.786666320351163	26.148185739659986
27	25.820517994753324	24.01125258987387	22.335551750524967	27.832677664847843
28	25.534408110176283	24.06409900361034	22.422863216698268	27.978629669515108
29	25.65188898456965	26.13400012387439	22.57620772536648	25.637903166189485
30	28.033174361197744	24.460097461174367	22.165524158503278	25.341204019124607
31	25.789249700803385	24.075587354422616	24.485172035555948	25.64999090921805
32	25.65858219765158	24.26679347141998	22.411774460696854	27.662849870231586
33	26.05737781889164	26.489739404244094	22.233754972457927	25.219127804406334
34	25.95548114212189	24.47368368474367	22.28879915765414	27.282036015480305
35	26.152181687768604	24.480776492636465	24.144517459296274	25.22252436029866
36	26.253978465835644	25.182864575320625	23.766101173410163	24.79705578543357
37	28.228276527601015	24.900550841446773	21.767827422993086	25.103345207959126
38	26.007128771425776	25.08646232720022	21.84844567608445	27.057963225289555
39	26.712451681304945	27.305992962075237	21.400489271908942	24.58106608471087
40	26.285546455893723	27.348868247596936	21.410489763379932	24.955095533129406
41	26.1838495765294	25.41183240194443	21.461338203062095	26.94297981846408
42	28.557942246561986	25.740099539067383	21.047857472522864	24.654100741847767
43	26.317514040762667	27.680132345801354	21.073331641715303	24.929021971720676
44	26.294237643029966	25.874463294219662	23.073203771375823	24.758095291374545
45	26.721903989354796	25.828509890970558	20.687622750530963	26.761963369143686
46	26.26876347383753	25.917919229900882	22.81906147166773	24.99425582459386
47	28.40979247043498	26.042592810889758	20.728081725130718	24.819532993544545
48	28.826969452974684	26.164369329499888	20.4401736639048	24.56848755362063
49	28.49840261974358	26.306025689950392	20.506306605102427	24.689265085203605
50	28.414687506868034	26.258573806160552	20.37523950713976	24.95149917983165
51	28.66423446625122	26.494534541974442	20.07684208212872	24.764388909645618
52	28.317286271720477	26.7239019634091	20.233183551878394	24.725628212992028
53	28.36214078923971	26.56855948068658	20.09792070840168	24.971379021672025
54	28.52717344612563	26.824400058340842	19.828793603286268	24.819632892247263
55	28.324478978315987	26.678647851079006	19.923397674757794	25.07347549584721
56	28.041865548333988	26.849075037911557	19.932388558002188	25.176670855752263
57	28.249355153873974	26.952270397816612	19.815107481014252	24.98326696729516
58	27.94466411059186	26.856567440615216	19.772950228468332	25.425818220324594
59	27.815195391872642	26.917205953163492	19.89282867172687	25.374769983237
60	28.063443668120524	26.92489815327258	19.52560104054489	25.48605713806201
61	28.05155572249739	26.941880932734207	19.55007622271017	25.456487122058235
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	4.0
2	3.0
3	3.0
4	3.0
5	3.0
6	4.5
7	9.5
8	13.0
9	13.5
10	14.0
11	16.0
12	19.5
13	21.0
14	28.0
15	35.0
16	51.5
17	93.5
18	119.0
19	176.5
20	303.0
21	372.0
22	501.0
23	630.0
24	794.0
25	1356.0
26	1754.0
27	2104.5
28	2455.0
29	3305.0
30	4798.0
31	5441.0
32	6627.0
33	7813.0
34	9312.0
35	12552.5
36	14294.0
37	16635.0
38	18976.0
39	21808.5
40	27450.0
41	30259.0
42	33759.5
43	40492.0
44	43724.0
45	47216.0
46	50708.0
47	53439.0
48	58215.0
49	60260.0
50	61828.0
51	63396.0
52	64733.5
53	67044.5
54	68018.0
55	67254.5
56	66491.0
57	65068.0
58	60312.5
59	56980.0
60	54237.0
61	56792.5
62	62091.0
63	48530.0
64	34969.0
65	31133.5
66	24205.0
67	21112.0
68	18486.0
69	15860.0
70	13517.5
71	9510.0
72	7845.0
73	6583.0
74	5321.0
75	4354.5
76	2704.5
77	2021.0
78	1640.5
79	1260.0
80	961.0
81	488.5
82	315.0
83	252.5
84	136.5
85	83.0
86	67.5
87	52.0
88	37.5
89	15.0
90	7.0
91	7.5
92	8.0
93	6.5
94	4.5
95	4.0
96	4.0
97	4.0
98	3.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03346606540967459
3	9.989870271544653E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.137818252292176
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1001014.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.439352470713
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.7507355227375	66.47792815820183
2	11.804116815272174	18.518145589572086
3	2.1431159417604917	5.043138802240658
4	0.5141580311366278	1.6132089211989515
5	0.20967780664173635	0.8223495690228582
6	0.11976827127168532	0.5636727387052616
7	0.08019734170177069	0.4403439287071581
8	0.06273718129383199	0.3936851101220728
9	0.04226439305508957	0.29836724614280336
>10	0.2685641317053835	3.519347341967391
>50	0.0034298260468401337	0.17853052057599958
>100	0.0010975443349888429	0.12397056039997077
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	1.3719304187360536E-4	2.0073115131429295
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCAACGATCTGGGGGGGGGGGGGGG	18653	1.8634105017512244	TruSeq Adapter, Index 10 (97% over 37bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0516196576671257	0.0	0.0	0.0	9.989870271544654E-5
2	2.054416821343158	0.0	0.0	0.0	9.989870271544654E-5
3	2.056814390208329	0.0	0.0	0.0	2.996961081463396E-4
4	2.0589122629653533	0.0	9.989870271544654E-5	0.0	2.996961081463396E-4
5	2.0608103383169465	0.0	9.989870271544654E-5	0.0	2.996961081463396E-4
6	2.06490618512828	0.0	9.989870271544654E-5	0.0	2.996961081463396E-4
7	2.069101930642329	0.0	9.989870271544654E-5	0.0	3.9959481086178616E-4
8	2.114555840377857	0.0	9.989870271544654E-5	0.0	5.993922162926792E-4
9	2.1196506742163446	0.0	9.989870271544654E-5	0.0	5.993922162926792E-4
10	2.1258443937847025	0.0	9.989870271544654E-5	9.989870271544654E-5	5.993922162926792E-4
11	2.132737404272068	0.0	9.989870271544654E-5	9.989870271544654E-5	5.993922162926792E-4
12	2.1492206902201167	0.0	9.989870271544654E-5	9.989870271544654E-5	7.991896217235723E-4
13	2.1715979996283767	0.0	9.989870271544654E-5	9.989870271544654E-5	7.991896217235723E-4
14	2.1986705480642628	0.0	9.989870271544654E-5	9.989870271544654E-5	7.991896217235723E-4
15	2.2373313460151407	0.0	9.989870271544654E-5	9.989870271544654E-5	7.991896217235723E-4
16	2.27968839596649	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	7.991896217235723E-4
17	2.3241433186748637	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	7.991896217235723E-4
18	2.3791875038710746	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	7.991896217235723E-4
19	2.467398058368814	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	7.991896217235723E-4
20	2.569194836435854	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	8.990883244390189E-4
21	2.6904718615324064	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	9.989870271544653E-4
22	2.8434167753897546	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	9.989870271544653E-4
23	3.0294281598459163	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.0010988857298699119
24	3.257097303334419	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.0010988857298699119
25	3.5387117462892625	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.0010988857298699119
26	3.8514945844913258	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.0010988857298699119
27	4.213227787023958	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.0010988857298699119
28	4.636798286537451	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.0010988857298699119
29	5.104723810056603	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.0010988857298699119
30	5.618303040716714	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.0010988857298699119
31	6.184229191599718	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.0010988857298699119
32	6.799205605516007	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.0010988857298699119
33	7.44415163024693	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.0010988857298699119
34	8.16921641455564	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.0010988857298699119
35	8.906568739298352	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.0010988857298699119
36	9.715748231293468	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.0010988857298699119
37	10.508044842529674	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.0010988857298699119
38	11.348292831069296	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.0011987844325853584
39	12.286841143080917	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.001298683135300805
40	13.255159268501739	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.001298683135300805
41	14.265834443873912	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.001298683135300805
42	15.350934152769092	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.001298683135300805
43	16.416953209445623	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.001298683135300805
44	17.526528100506088	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.001298683135300805
45	18.65078810086572	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.001298683135300805
46	19.805417306850853	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.001298683135300805
47	20.989117035326178	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.001298683135300805
48	22.181208254829603	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.001298683135300805
49	23.439732111638797	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.001298683135300805
50	24.670284331687668	9.989870271544654E-5	9.989870271544654E-5	9.989870271544654E-5	0.001298683135300805
>>END_MODULE