##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-25C_S15_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1201656
Sequences flagged as poor quality	0
Sequence length	61
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.47669549355223	40.0	40.0	40.0	40.0	40.0
2	39.203126352300494	40.0	40.0	40.0	40.0	40.0
3	39.16560812745078	40.0	40.0	40.0	40.0	40.0
4	39.06208099489371	40.0	40.0	40.0	40.0	40.0
5	39.11667898300345	40.0	40.0	40.0	40.0	40.0
6	39.22085521979668	40.0	40.0	40.0	40.0	40.0
7	39.23214630476609	40.0	40.0	40.0	40.0	40.0
8	39.21759305491755	40.0	40.0	40.0	40.0	40.0
9	39.254185890138274	40.0	40.0	40.0	40.0	40.0
10	39.26849947073039	40.0	40.0	40.0	40.0	40.0
11	39.25340696505489	40.0	40.0	40.0	40.0	40.0
12	39.264588201615105	40.0	40.0	40.0	40.0	40.0
13	39.084047347993106	40.0	40.0	40.0	40.0	40.0
14	39.260081088098424	40.0	40.0	40.0	40.0	40.0
15	39.16943617807426	40.0	40.0	40.0	40.0	40.0
16	39.346308760577074	40.0	40.0	40.0	40.0	40.0
17	39.29432050437064	40.0	40.0	40.0	40.0	40.0
18	39.37684994707304	40.0	40.0	40.0	40.0	40.0
19	39.32655601935995	40.0	40.0	40.0	40.0	40.0
20	39.33865265933012	40.0	40.0	40.0	40.0	40.0
21	39.317701571830874	40.0	40.0	40.0	40.0	40.0
22	39.372063219423865	40.0	40.0	40.0	40.0	40.0
23	39.367489531113726	40.0	40.0	40.0	40.0	40.0
24	39.360805421851175	40.0	40.0	40.0	40.0	40.0
25	39.32788751522899	40.0	40.0	40.0	40.0	40.0
26	39.29008301876743	40.0	40.0	40.0	40.0	40.0
27	39.311560047134954	40.0	40.0	40.0	40.0	40.0
28	39.31894319172875	40.0	40.0	40.0	40.0	40.0
29	39.32503478532958	40.0	40.0	40.0	40.0	40.0
30	39.31441610577403	40.0	40.0	40.0	40.0	40.0
31	39.26980100794237	40.0	40.0	40.0	40.0	40.0
32	39.30110114708369	40.0	40.0	40.0	40.0	40.0
33	39.297319698815635	40.0	40.0	40.0	40.0	40.0
34	39.292463067633335	40.0	40.0	40.0	40.0	40.0
35	39.30684988049825	40.0	40.0	40.0	40.0	40.0
36	39.27197800368825	40.0	40.0	40.0	40.0	40.0
37	39.23921987657033	40.0	40.0	40.0	40.0	40.0
38	39.26318680221295	40.0	40.0	40.0	40.0	40.0
39	38.04702177661494	40.0	40.0	40.0	40.0	40.0
40	39.26461150279281	40.0	40.0	40.0	40.0	40.0
41	39.27816945947925	40.0	40.0	40.0	40.0	40.0
42	39.241127244402726	40.0	40.0	40.0	40.0	40.0
43	39.261945182315074	40.0	40.0	40.0	40.0	40.0
44	39.21928072593155	40.0	40.0	40.0	40.0	40.0
45	39.01756243051256	40.0	40.0	40.0	40.0	40.0
46	39.06621528956706	40.0	40.0	40.0	40.0	40.0
47	38.98823290525741	40.0	40.0	40.0	40.0	40.0
48	39.2420160178953	40.0	40.0	40.0	40.0	40.0
49	38.82307082892275	40.0	40.0	40.0	40.0	40.0
50	39.16770856218419	40.0	40.0	40.0	40.0	40.0
51	39.08073192327921	40.0	40.0	40.0	40.0	40.0
52	39.1420140206515	40.0	40.0	40.0	40.0	40.0
53	38.93099189784764	40.0	40.0	40.0	40.0	40.0
54	38.708116133069694	40.0	40.0	40.0	40.0	40.0
55	38.81315118469845	40.0	40.0	40.0	40.0	40.0
56	38.7533570339598	40.0	40.0	40.0	40.0	40.0
57	38.585069271072584	40.0	40.0	40.0	40.0	40.0
58	38.455694474957895	40.0	40.0	40.0	40.0	40.0
59	38.36633112970767	40.0	40.0	40.0	40.0	40.0
60	38.330813477401186	40.0	40.0	40.0	40.0	40.0
61	38.21528290958477	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.028615599138497316
1101	2	0.010525017324219732
1101	3	0.009463431835904146
1101	4	0.04843630899021889
1101	5	-0.0047155944485695045
1101	6	0.025902571916361694
1101	7	-0.10986728335360851
1101	8	0.09199787712537244
1101	9	-0.0779635117328965
1101	10	0.023797201299046833
1101	11	-0.0370979219852714
1101	12	-0.06741470031131058
1101	13	-0.008663993508399415
1101	14	0.036592160536699225
1101	15	0.014237102717338246
1101	16	0.06901600776711803
1101	17	-0.024176787913440023
1101	18	-0.038868494314939994
1101	19	0.022813561422914574
1101	20	-0.06555277133161042
1101	21	-0.007625545561730007
1101	22	-0.01598476128553017
1101	23	0.034766003967689585
1101	24	0.03745440952700818
1101	25	0.025004276474561493
1101	26	0.10500809672505085
1101	27	0.0072682549549227815
1101	28	0.04276253580673739
1101	29	0.013915467385452018
1101	30	0.05414690722765414
1101	31	0.021470596621277593
1101	32	0.037858173697081554
1101	33	0.10176502172586055
1101	34	-0.00798177449213
1101	35	0.0552009503406623
1101	36	-0.07853301884417618
1101	37	-0.06327903063931473
1101	38	-0.08043510997651282
1101	39	1.1522227506947829
1101	40	-0.0554014608309501
1101	41	0.04006661159615987
1101	42	-0.03793627680081357
1101	43	-0.014984197500325536
1101	44	-0.06196449447409691
1101	45	-0.10338921890124908
1101	46	0.04111526301745272
1101	47	0.03960086805063412
1101	48	0.021748892304628953
1101	49	-0.016978185080404273
1101	50	-0.021335356850599396
1101	51	0.10324560159837404
1101	52	0.06313172931699285
1101	53	0.05034275670124089
1101	54	0.09847284349339702
1101	55	0.14468681272633432
1101	56	-0.022996272874564738
1101	57	0.07596887947669018
1101	58	0.03284336250608533
1101	59	0.19474691247911835
1101	60	0.0836642404904211
1101	61	0.10039146571669022
1102	1	0.010891391565905906
1102	2	-0.015042849925784196
1102	3	0.04876739862238111
1102	4	-0.08411310083768342
1102	5	-0.08499949181929622
1102	6	-0.10553766051214097
1102	7	-0.12631818402490325
1102	8	0.01329371726287576
1102	9	-0.10640556338039175
1102	10	-0.05913928894327114
1102	11	-0.02767587740973454
1102	12	-0.07080728690243632
1102	13	-0.08818810978173985
1102	14	-0.10600381603884301
1102	15	-0.07388124098154947
1102	16	-0.0643002307174072
1102	17	-0.08444886978005428
1102	18	-0.09968188294269709
1102	19	9.181501836081907E-4
1102	20	-0.06409961882960857
1102	21	-0.11556948656497923
1102	22	0.011248905013353294
1102	23	-0.01714577522793803
1102	24	-0.012290787324197083
1102	25	-0.09213049494937309
1102	26	-0.02959730297930463
1102	27	-0.12994067576634905
1102	28	0.025099454947238087
1102	29	-0.12941508171021354
1102	30	-0.11306998859085127
1102	31	-0.09919554409869136
1102	32	-0.11187969098192241
1102	33	-0.030707407070416082
1102	34	-0.045253333619591274
1102	35	-0.04304798869673476
1102	36	-0.06212874048781458
1102	37	-0.08987380858766159
1102	38	-0.12263643678796399
1102	39	1.217697286575742
1102	40	-0.08483857363025038
1102	41	-0.058594067536461125
1102	42	-0.042357535980087846
1102	43	-0.04510546566523743
1102	44	-0.03114199416543073
1102	45	-0.08625172332460807
1102	46	-0.09586814580834613
1102	47	-0.07491980101752915
1102	48	-0.08755983565023939
1102	49	0.07678525356469379
1102	50	-0.0784506743004556
1102	51	-0.06016398587410521
1102	52	-0.059352952745243215
1102	53	-0.017585142972961876
1102	54	0.07009178033926844
1102	55	-0.032222916578270144
1102	56	-0.12343618965373082
1102	57	-0.15440444735481407
1102	58	0.01334927154567822
1102	59	-0.11077128574189032
1102	60	-0.023226361467365564
1102	61	0.04487567315291585
1103	1	0.008587868824584177
1103	2	-0.028056018180549813
1103	3	-0.037431420117272296
1103	4	-0.06382388076016099
1103	5	0.06352187216115368
1103	6	0.0599434438773514
1103	7	-0.09142046520651093
1103	8	-0.010875919183973792
1103	9	-0.06364832040646462
1103	10	0.07029938464408758
1103	11	0.05398801649808149
1103	12	-0.10185604046237273
1103	13	-0.026535256298366505
1103	14	-0.028941002747821187
1103	15	-0.11356657803985115
1103	16	-0.16246294358177948
1103	17	-0.108390756803324
1103	18	-0.03074267468645786
1103	19	-0.0997584825086335
1103	20	-0.08441269837055643
1103	21	-0.03995884697953045
1103	22	-0.048308111512575636
1103	23	-0.08779635772420136
1103	24	-0.09029225359667947
1103	25	-0.051927841343236025
1103	26	-0.0974330110522601
1103	27	-0.048923698673604576
1103	28	-0.07773320562152719
1103	29	0.025155740768873613
1103	30	-0.06531310350887054
1103	31	-0.0461380623132257
1103	32	-0.09509672750642295
1103	33	-0.09548801930424133
1103	34	-0.1471749964940514
1103	35	0.010428660480222618
1103	36	-0.05384787989865458
1103	37	-0.001253796571170085
1103	38	-0.08687218563891719
1103	39	1.1862418376165564
1103	40	-0.034860589407863074
1103	41	-0.07629562035372572
1103	42	-0.05163019108287159
1103	43	-0.025552093130087883
1103	44	-0.03313697066386112
1103	45	-0.10575195432759443
1103	46	-0.06389158447759513
1103	47	0.07565564585937068
1103	48	-0.032378861620181
1103	49	0.0013822997631223188
1103	50	-0.020519897110965246
1103	51	0.015897814474243432
1103	52	0.012095837254598507
1103	53	0.012731558868338766
1103	54	-0.09270459215466786
1103	55	-0.1398204204445932
1103	56	-0.11980629765033513
1103	57	-0.11836093203161369
1103	58	-0.09202767544076806
1103	59	-0.05562881220962623
1103	60	-0.013218401082752962
1103	61	0.0698700878694396
1104	1	0.04207517549638595
1104	2	0.11633160491783201
1104	3	0.02411587661681125
1104	4	0.012692244221298665
1104	5	0.058672902793801995
1104	6	0.022322727084002736
1104	7	-0.043302624181905
1104	8	0.008420364562873317
1104	9	0.03912103460510252
1104	10	-0.0452093065320156
1104	11	-0.09933191859279589
1104	12	0.06424734431688961
1104	13	0.06493941279607185
1104	14	0.041627103661575404
1104	15	-0.06702139767237725
1104	16	-0.001421788641010835
1104	17	-0.003696103578924692
1104	18	0.016439580743295323
1104	19	-0.01770489242557005
1104	20	0.0937550585947804
1104	21	0.015266954722875425
1104	22	0.020714397751262936
1104	23	0.019526974378642592
1104	24	-0.00798967920720628
1104	25	0.04389133326296246
1104	26	0.1262160596408819
1104	27	0.12930630436833468
1104	28	0.05759220062884651
1104	29	0.04632346634053164
1104	30	0.09293969783521305
1104	31	0.0682288193098941
1104	32	-0.016453951786076004
1104	33	0.1063083483394891
1104	34	0.058415946106407546
1104	35	-0.02708623798289267
1104	36	-0.007449925034102023
1104	37	-0.09978744138747686
1104	38	-0.029225282191781332
1104	39	1.2482995521787785
1104	40	-0.048896267776434854
1104	41	0.03990319036732615
1104	42	0.07286737482490224
1104	43	0.12850775836701445
1104	44	-0.04114154547985294
1104	45	-0.04251183494503863
1104	46	-0.027174247700337162
1104	47	0.038727754962131655
1104	48	-0.007783812943742419
1104	49	0.06419914582478725
1104	50	0.022355200452992108
1104	51	0.07519613339715647
1104	52	0.05189624926950387
1104	53	0.04285757740359486
1104	54	0.032643309286513045
1104	55	0.011778545945773544
1104	56	0.04296069742301967
1104	57	0.14169857521761742
1104	58	0.06413338709435124
1104	59	0.06983925122291623
1104	60	0.150998656940331
1104	61	0.11902689710307612
1201	1	0.0490871837755833
1201	2	0.038024611326278546
1201	3	0.06364207662901578
1201	4	-0.033885828296917
1201	5	0.009237438949632804
1201	6	0.061003587053676256
1201	7	0.1521899058490277
1201	8	0.04703514821138555
1201	9	0.039246907266480946
1201	10	0.15564823851694598
1201	11	-0.0881292517306278
1201	12	0.01102505196821113
1201	13	-0.02433788836300721
1201	14	0.03335937223779695
1201	15	0.1093745294596431
1201	16	0.04170065610642126
1201	17	0.12508610026570466
1201	18	-0.04602165573307104
1201	19	0.02639204153756225
1201	20	0.003171521400979316
1201	21	-0.00545452498261767
1201	22	0.06607715743700737
1201	23	-0.00840938429455207
1201	24	0.015295944018788532
1201	25	-0.025952976453133658
1201	26	0.007296975420466367
1201	27	0.09631428799887942
1201	28	-0.008023770618670767
1201	29	0.02568608377105619
1201	30	0.08493627169604423
1201	31	-0.02470015424223959
1201	32	0.09029316416680899
1201	33	0.06794230654457323
1201	34	-0.018844475009998973
1201	35	0.09327833402343799
1201	36	0.10972086710221873
1201	37	0.07249427910340245
1201	38	0.03972508589018986
1201	39	1.396915574075706
1201	40	0.08698367973931198
1201	41	0.1331469820942104
1201	42	0.04994162181081663
1201	43	0.05036295210920372
1201	44	0.13022875864733408
1201	45	0.04761162538453334
1201	46	0.17514871724795
1201	47	0.031739228350780024
1201	48	0.005767293745897462
1201	49	0.09341716669425182
1201	50	0.07130055274149072
1201	51	0.0497507428144246
1201	52	0.13330336910232177
1201	53	0.16755697451177554
1201	54	0.09813095048881593
1201	55	0.14440000248360008
1201	56	0.21000191034399762
1201	57	0.16106794772328925
1201	58	0.11118054688423484
1201	59	0.2327616157777186
1201	60	0.20630126123477766
1201	61	0.19061513022087695
1202	1	0.03636819178665007
1202	2	0.018745557797565482
1202	3	-0.006258730090181075
1202	4	0.05306552589193103
1202	5	-0.027440469292045577
1202	6	0.055572635250484836
1202	7	0.029443792115984024
1202	8	0.07409251325884014
1202	9	0.022343276122199995
1202	10	0.047866130284184294
1202	11	0.05728984772181889
1202	12	0.009686297587549575
1202	13	-0.11495059546962949
1202	14	-0.022524956742522306
1202	15	-0.09064792067547245
1202	16	0.023040632038551223
1202	17	-0.018561749556099016
1202	18	-0.10914780403272317
1202	19	-0.007103229902725161
1202	20	-0.08089598087292416
1202	21	-0.04755513130133693
1202	22	0.007711758377602962
1202	23	-0.05667057180765056
1202	24	-0.0023274120453109504
1202	25	0.06957234845479121
1202	26	-0.04338420459825443
1202	27	0.02518697757459165
1202	28	-0.04700446845470907
1202	29	-0.04992465069501861
1202	30	-0.07461320691607654
1202	31	-0.12391489473683492
1202	32	-0.041986003263325244
1202	33	-0.07599772518274506
1202	34	-0.004469785556445061
1202	35	-0.012628527629253483
1202	36	-0.25496507036424987
1202	37	-0.19788128194362997
1202	38	-0.2622259298958909
1202	39	1.0461272606916339
1202	40	-0.11904583228389498
1202	41	-0.35444354064728145
1202	42	-0.14061710592682175
1202	43	-0.18631429583437864
1202	44	-0.24823594924504278
1202	45	-0.12517352847317653
1202	46	-0.05668689493917611
1202	47	-0.20739102700299838
1202	48	-0.20064354042519028
1202	49	-0.17778376696724507
1202	50	-0.16995421172192948
1202	51	-0.09315097016389728
1202	52	-0.0751708966049307
1202	53	-0.16693518197227064
1202	54	-0.13142993477283937
1202	55	-0.08248832625240965
1202	56	-0.1809539866957195
1202	57	-0.11614334931676495
1202	58	-0.15949214830401104
1202	59	-0.2796973838376573
1202	60	-0.09081307103052438
1202	61	-0.07878649114108072
1203	1	0.02812786210757423
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2403	5	0.02529281214786039
2403	6	0.06841284446293372
2403	7	0.08928852151907307
2403	8	0.06448198708679342
2403	9	0.05707431795103446
2403	10	0.07609683753697283
2403	11	0.0558696065539408
2403	12	0.05441023821054358
2403	13	0.006821428510598082
2403	14	0.03333558649972446
2403	15	0.03626906349703063
2403	16	-0.00507399781109541
2403	17	0.11289590950408979
2403	18	0.03165067193925353
2403	19	0.01884006969987695
2403	20	0.05551203802721005
2403	21	0.037550089450569146
2403	22	0.03627362763347719
2403	23	-0.06606601337975349
2403	24	0.07014585601155687
2403	25	0.09841075505345032
2403	26	-0.011517543394056418
2403	27	0.07521633832950414
2403	28	-0.01829920946553898
2403	29	0.118390997904541
2403	30	-0.04939368406247979
2403	31	0.10032957793034569
2403	32	0.026892279337353386
2403	33	0.06446958878614595
2403	34	0.01978356361803435
2403	35	-0.07990562487480446
2403	36	-0.009017537350473503
2403	37	-0.024789389608834256
2403	38	0.1110585143664764
2403	39	1.2332815890131528
2403	40	0.02904162179725489
2403	41	-0.05612802860937194
2403	42	0.10345953247158235
2403	43	0.07356593959790558
2403	44	-0.0401603760275151
2403	45	0.027881355654258755
2403	46	0.06440232078726638
2403	47	0.009130884313861998
2403	48	0.033822571801174206
2403	49	0.05403987731696702
2403	50	0.06152461439412349
2403	51	0.04077162669245382
2403	52	-0.0840269196565373
2403	53	0.04990082114133543
2403	54	0.04273396652040162
2403	55	0.05630952153597235
2403	56	0.11736835342473029
2403	57	0.006960150758345662
2403	58	0.09184274183213859
2403	59	-0.007973839243447856
2403	60	-0.030592797087855672
2403	61	0.006192410083876609
2404	1	0.0719392475105991
2404	2	-0.02513700554278131
2404	3	-0.01600049438616935
2404	4	0.03363005392439078
2404	5	0.12297333257049559
2404	6	0.05700975716212753
2404	7	-0.03513973430199968
2404	8	0.03342173431816775
2404	9	0.040395403829244
2404	10	-0.05031927446128748
2404	11	0.199070620266383
2404	12	0.03871936397777631
2404	13	0.05091226694310791
2404	14	0.07849807725525437
2404	15	0.05591563020562518
2404	16	0.05664022195535523
2404	17	0.05635623952589697
2404	18	0.05343347745437654
2404	19	0.08281300282406079
2404	20	-0.02393743886513988
2404	21	0.007996038133732952
2404	22	-0.0158840442195185
2404	23	0.10095630054136961
2404	24	0.07254824143896599
2404	25	-0.016806001349372934
2404	26	0.05959542053719247
2404	27	0.04796810596701562
2404	28	-0.005745567967387899
2404	29	0.0076563390197179615
2404	30	0.007508699009655118
2404	31	0.03710797907218932
2404	32	0.09280596304211741
2404	33	-0.1610201916546714
2404	34	0.08343264576548393
2404	35	0.05297219733925118
2404	36	0.014134863386260577
2404	37	0.11562297278393885
2404	38	0.11851297503094571
2404	39	1.3804995324770175
2404	40	0.059999419893244976
2404	41	0.1550310517009592
2404	42	0.018245508193359683
2404	43	-0.022523589299019875
2404	44	-0.04135332526180946
2404	45	0.14007157747451515
2404	46	0.11618079258749958
2404	47	0.12772040143439511
2404	48	0.03760632884934978
2404	49	0.193697372523971
2404	50	0.08996461835099723
2404	51	0.07518108738047857
2404	52	0.025104971303427703
2404	53	0.21187459274513998
2404	54	0.11217939233144136
2404	55	0.14473025930382732
2404	56	0.002248163494961375
2404	57	0.0036698248347235563
2404	58	0.024140223095891145
2404	59	-0.028928763898051102
2404	60	-0.019133183071112114
2404	61	-0.0013409524549317098
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	3.0
17	36.0
18	90.0
19	132.0
20	192.0
21	277.0
22	397.0
23	614.0
24	945.0
25	1223.0
26	1571.0
27	2074.0
28	2860.0
29	3421.0
30	4383.0
31	5521.0
32	7388.0
33	8904.0
34	11860.0
35	17755.0
36	34099.0
37	35867.0
38	74699.0
39	508101.0
40	479243.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	29.147776069024744	16.037618087039885	24.245125060749498	30.56948078318587
2	29.661547477922667	18.40876699921414	26.729141414814123	25.200544108049073
3	27.53729442917845	21.496537272792335	20.47194949627763	30.49421880175159
4	27.6309526187195	20.698519376593634	22.37603773459293	29.294490270093938
5	27.585681759172342	20.635439759798146	21.232449220076294	30.546429260953218
6	29.795632027801634	23.46120686785569	22.677122237978256	24.066038866364416
7	28.69681506188127	23.76029412743747	21.961942519323333	25.580948291357924
8	25.362166876377266	26.641151877076304	22.216923978243358	25.779757268303076
9	25.09836425732489	26.135932413269686	23.225948191495736	25.539755137909687
10	27.261795389029807	22.637427017382677	23.11851311856305	26.982264475024465
11	25.30865738614046	25.019556345576436	22.709827105261407	26.9619591630217
12	27.846405294027576	23.06458753586717	22.699757667751836	26.38924950235342
13	25.421501661041095	22.041332960514488	22.71956366880372	29.817601709640694
14	25.600338199950734	24.71505988402671	22.759092452415665	26.92550946360689
15	25.73997882921568	22.799286983962133	22.541975407271302	28.918758779550885
16	25.703196255833618	24.825823696631982	22.321862496421605	27.149117551112802
17	25.90583328340224	22.362306683443514	22.321113529995273	29.410746503158975
18	28.08898719766722	23.024559441304334	22.480976252771175	26.40547710825727
19	25.70161510448914	22.44469299034	24.692258017269502	27.161433887901364
20	25.778009679974968	22.678953044798178	22.272347493791898	29.270689781434957
21	25.69554015458667	22.991355263070297	24.767820407837185	26.545284174505852
22	28.299779637433677	22.239143315557865	22.334428488685614	27.126648558322845
23	25.817538463586914	24.911289087725606	22.397175231513845	26.873997217173635
24	25.946360688915966	25.50372153095395	22.252125400280946	26.297792379849145
25	25.989967178626827	22.425968829681704	22.19071015332175	29.39335383836972
26	26.174379356488046	22.83648564980327	24.504184225768437	26.48495076794024
27	26.112131924610704	23.546089729506615	21.960943897421558	28.380834448461123
28	26.00586191056342	23.05468453534123	22.060057121172783	28.879396432922565
29	26.102645016543836	25.935126192520986	21.936810534795313	26.025418256139858
30	28.569074676945817	24.004540400913406	21.857503312095975	25.5688816100448
31	26.040231147682864	23.51704647586331	24.317525148628228	26.125197227825602
32	26.367529475989798	23.644453986831508	21.775033786707677	28.212982750471017
33	26.332744146411287	26.567004200869466	21.728015338832414	25.372236313886837
34	26.61077712756396	23.848089636301903	21.47270100594513	28.068432230189007
35	26.747005798664507	24.281325104688865	23.662262744079836	25.309406352566793
36	26.82689555080655	27.317884652512863	21.110534129567863	24.744685667112716
37	26.762734093617475	24.654060729526588	20.943348179512274	27.639856997343664
38	26.975440558695666	24.82507473020565	20.660571744326166	27.53891296677252
39	29.56808177747856	25.20089718907092	20.574122003287172	24.656899030163345
40	29.376793358498603	25.02313474072447	20.372469325663918	25.22760257511301
41	26.994830467288477	27.875198892195435	20.27460437928991	24.855366261226173
42	27.066814462708127	25.62239110028161	22.611129973969256	24.699664463041003
43	27.08628758979275	28.007682731164323	19.829385448081645	25.076644230961275
44	27.23549834561638	25.968912900197726	21.95969562004434	24.83589313414155
45	27.089699547957153	26.274657639124676	19.676762734093618	26.958880078824553
46	27.01072519922507	26.13917793445046	21.782357013987365	25.067739852337105
47	29.56594899039326	26.304283422210684	19.266162695480237	24.863604891915823
48	29.517931920616213	26.6297509436977	19.204248137570154	24.64806899811593
49	29.25121665435033	26.619098976745427	18.991208798524703	25.138475570379544
50	29.357403449905796	26.802762188180314	18.77259382052767	25.06724054138622
51	29.21526626588641	26.97144607108856	18.801387418695533	25.011900244329492
52	29.20752694614765	26.79510608693337	18.55106619531713	25.446300771601855
53	29.053156643831514	27.01422037588128	18.585185776961126	25.347437203326077
54	28.918009813124556	27.166926308361127	18.658834142217074	25.256229736297243
55	28.794513571271647	26.844121778612184	18.430232945202288	25.931131704913884
56	28.59953264494997	27.16043526599959	18.530344790855285	25.709687298195156
57	28.429600484664498	27.102348758712974	18.587515894731936	25.88053486189059
58	28.153980839774444	27.057993302575778	18.485073931308126	26.30295192634165
59	27.73089802738887	27.22492959715592	18.78332900597176	26.260843369483446
60	27.855975420586258	27.235997656567267	18.55847264108863	26.349554281757843
61	27.563712077333278	27.168091367246532	18.656504024446264	26.611692530973922
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	4.5
2	3.0
3	5.0
4	5.5
5	6.0
6	4.5
7	9.0
8	15.0
9	18.0
10	21.0
11	18.5
12	15.0
13	14.0
14	23.0
15	32.0
16	44.5
17	70.0
18	83.0
19	132.5
20	222.0
21	262.0
22	354.5
23	447.0
24	597.0
25	991.0
26	1235.0
27	1662.0
28	2089.0
29	2706.0
30	3962.0
31	4601.0
32	5682.0
33	6763.0
34	8027.5
35	11016.5
36	12741.0
37	14874.5
38	17008.0
39	19999.0
40	26245.0
41	29500.0
42	33428.0
43	40663.5
44	43971.0
45	48529.5
46	53088.0
47	56999.0
48	64467.5
49	68025.0
50	72055.0
51	76085.0
52	78965.5
53	84525.5
54	87205.0
55	88561.5
56	89918.0
57	89577.5
58	87251.5
59	85266.0
60	83196.0
61	78931.5
62	76737.0
63	62254.5
64	47772.0
65	41704.5
66	30926.0
67	26215.0
68	21989.0
69	17763.0
70	15047.0
71	10162.5
72	7994.0
73	6507.5
74	5021.0
75	4058.5
76	2413.0
77	1730.0
78	1300.5
79	871.0
80	706.5
81	387.5
82	233.0
83	174.5
84	80.5
85	45.0
86	38.5
87	32.0
88	24.0
89	12.5
90	9.0
91	6.5
92	4.0
93	4.5
94	4.5
95	4.0
96	3.5
97	3.0
98	3.5
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03528464052940276
3	1.664369836292583E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.0899026010771804
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1201656.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.29022576800226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.5861850023136	59.06179692601562
2	14.227642559517209	20.854942706669075
3	3.194166792814648	7.023036161581239
4	0.8738113220951037	2.561673162999468
5	0.33797252850980575	1.2385041458933124
6	0.1593276632104941	0.7006296244671205
7	0.09826976992909316	0.5041549536991015
8	0.08051469917190604	0.47207523839614224
9	0.05802918656185384	0.3827674965826611
>10	0.37969544403688765	4.701774810510226
>50	0.002381111322723715	0.11149247875809778
>100	0.0012524503229441285	0.19891405914117272
>500	2.504900645888257E-4	0.13245638548256217
>1k	3.7573509688323855E-4	0.5707649375818237
>5k	0.0	0.0
>10k+	1.2524503229441284E-4	1.4850169122223775
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCGGATATCTGGGGGGGGGGGGGGG	16178	1.3463087605770703	TruSeq Adapter, Index 10 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCGGATATCTGGGGGGGGGGGGGGT	2716	0.22602142376853276	TruSeq Adapter, Index 10 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCGGATATCTGGGGGGGGGGGGTGG	2400	0.19972438035510995	TruSeq Adapter, Index 10 (97% over 37bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.429730305511727	0.0	0.0	0.0	8.321849181462914E-5
2	2.4368870958077853	0.0	0.0	0.0	8.321849181462914E-5
3	2.441880205316663	0.0	0.0	0.0	8.321849181462914E-5
4	2.4453753819728776	0.0	0.0	0.0	8.321849181462914E-5
5	2.4520328613180475	0.0	0.0	0.0	8.321849181462914E-5
6	2.4639331056475395	0.0	0.0	0.0	8.321849181462914E-5
7	2.475334039026144	0.0	0.0	0.0	8.321849181462914E-5
8	2.5726164559574456	0.0	0.0	0.0	8.321849181462914E-5
9	2.588427969402225	0.0	0.0	0.0	1.664369836292583E-4
10	2.6025751130107118	0.0	0.0	0.0	1.664369836292583E-4
11	2.6217153661280768	0.0	0.0	0.0	1.664369836292583E-4
12	2.6578321915756256	0.0	0.0	0.0	1.664369836292583E-4
13	2.709760530467954	0.0	0.0	0.0	1.664369836292583E-4
14	2.7649343905410535	0.0	0.0	0.0	1.664369836292583E-4
15	2.838998848256073	0.0	0.0	0.0	1.664369836292583E-4
16	2.9218844661034438	0.0	0.0	0.0	1.664369836292583E-4
17	3.0108450338532826	0.0	0.0	0.0	1.664369836292583E-4
18	3.1002216940621943	0.0	0.0	0.0	1.664369836292583E-4
19	3.231790129621123	0.0	0.0	0.0	1.664369836292583E-4
20	3.3854114655109284	0.0	0.0	0.0	1.664369836292583E-4
21	3.598450804556379	0.0	0.0	0.0	2.4965547544388743E-4
22	3.849520994361115	0.0	0.0	0.0	3.328739672585166E-4
23	4.144946640303049	0.0	0.0	0.0	3.328739672585166E-4
24	4.492550280612754	0.0	0.0	0.0	5.825294427024041E-4
25	4.90090341994714	0.0	0.0	0.0	5.825294427024041E-4
26	5.375415260274155	0.0	0.0	0.0	5.825294427024041E-4
27	5.943298248417184	0.0	0.0	0.0	5.825294427024041E-4
28	6.567520155518718	0.0	0.0	0.0	5.825294427024041E-4
29	7.242671779610804	0.0	0.0	0.0	5.825294427024041E-4
30	8.00195729892748	0.0	0.0	0.0	5.825294427024041E-4
31	8.817498518710845	0.0	0.0	0.0	5.825294427024041E-4
32	9.68621635476376	0.0	0.0	0.0	6.657479345170332E-4
33	10.657792246699554	0.0	0.0	0.0	6.657479345170332E-4
34	11.699687764218712	0.0	0.0	0.0	6.657479345170332E-4
35	12.776618266791827	0.0	0.0	0.0	6.657479345170332E-4
36	13.940761748786674	0.0	0.0	0.0	6.657479345170332E-4
37	15.178636814529282	0.0	0.0	0.0	6.657479345170332E-4
38	16.46952205955781	0.0	0.0	0.0	7.489664263316624E-4
39	17.85411132636961	0.0	0.0	0.0	7.489664263316624E-4
40	19.294540201189026	0.0	0.0	0.0	8.321849181462914E-4
41	20.745870698436157	0.0	0.0	0.0	9.154034099609206E-4
42	22.264025644610438	0.0	0.0	0.0	9.154034099609206E-4
43	23.8122224663298	0.0	0.0	0.0	9.154034099609206E-4
44	25.341362253423608	0.0	0.0	0.0	9.154034099609206E-4
45	26.91851911029446	0.0	0.0	0.0	9.154034099609206E-4
46	28.528047960481203	0.0	0.0	0.0	9.986219017755497E-4
47	30.116356095255213	0.0	0.0	0.0	9.986219017755497E-4
48	31.736037601443343	0.0	0.0	0.0	9.986219017755497E-4
49	33.334082299759665	0.0	0.0	0.0	0.0010818403935901788
50	34.90408236633446	0.0	0.0	0.0	0.0010818403935901788
>>END_MODULE