##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-25C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 581327 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.5807179091974 40.0 40.0 40.0 40.0 40.0 2 39.35357896674333 40.0 40.0 40.0 40.0 40.0 3 39.330511054879615 40.0 40.0 40.0 40.0 40.0 4 39.21324830947126 40.0 40.0 40.0 40.0 40.0 5 39.27689579186929 40.0 40.0 40.0 40.0 40.0 6 39.39642232340834 40.0 40.0 40.0 40.0 40.0 7 39.39631911127472 40.0 40.0 40.0 40.0 40.0 8 39.4001310794097 40.0 40.0 40.0 40.0 40.0 9 39.42201893254571 40.0 40.0 40.0 40.0 40.0 10-11 39.42437216919221 40.0 40.0 40.0 40.0 40.0 12-13 39.33922903976591 40.0 40.0 40.0 40.0 40.0 14-15 39.376302838161656 40.0 40.0 40.0 40.0 40.0 16-17 39.47622594512211 40.0 40.0 40.0 40.0 40.0 18-19 39.51274583840076 40.0 40.0 40.0 40.0 40.0 20-21 39.48635449583453 40.0 40.0 40.0 40.0 40.0 22-23 39.52468060145151 40.0 40.0 40.0 40.0 40.0 24-25 39.499840881294006 40.0 40.0 40.0 40.0 40.0 26-27 39.4751731813592 40.0 40.0 40.0 40.0 40.0 28-29 39.4922960743265 40.0 40.0 40.0 40.0 40.0 30-31 39.463947771436224 40.0 40.0 40.0 40.0 40.0 32-33 39.46997919138832 40.0 40.0 40.0 40.0 40.0 34-35 39.465110853180924 40.0 40.0 40.0 40.0 40.0 36-37 39.45298217108456 40.0 40.0 40.0 40.0 40.0 38-39 39.39166837750065 40.0 40.0 40.0 40.0 40.0 40-41 39.44039612213899 40.0 40.0 40.0 40.0 40.0 42-43 39.41359854426089 40.0 40.0 40.0 40.0 40.0 44-45 39.31860770943365 40.0 40.0 40.0 40.0 40.0 46-47 39.30484422347635 40.0 40.0 40.0 40.0 40.0 48-49 39.38263156286504 40.0 40.0 40.0 40.0 40.0 50-51 39.34404835701852 40.0 40.0 40.0 40.0 40.0 52-53 39.30433208957827 40.0 40.0 40.0 40.0 40.0 54-55 39.20059318054929 40.0 40.0 40.0 40.0 40.0 56-57 39.22495361390397 40.0 40.0 40.0 40.0 40.0 58-59 39.16958972162864 40.0 40.0 40.0 40.0 40.0 60-61 39.10019228085055 40.0 40.0 40.0 40.0 40.0 62-63 39.39810946925141 40.0 40.0 40.0 40.0 40.0 64-65 39.40171693615828 40.0 40.0 40.0 40.0 40.0 66-67 39.40003115524968 40.0 40.0 40.0 40.0 40.0 68-69 39.40312984653494 40.0 40.0 40.0 40.0 40.0 70-71 39.40044410267346 40.0 40.0 40.0 40.0 40.0 72-73 39.40331474693343 40.0 40.0 40.0 40.0 40.0 74-75 39.40490327696587 40.0 40.0 40.0 40.0 40.0 76-77 39.3902935848197 40.0 40.0 40.0 40.0 40.0 78-79 39.39470017787704 40.0 40.0 40.0 40.0 40.0 80-81 39.39111511830522 40.0 40.0 40.0 40.0 40.0 82-83 39.376896296614106 40.0 40.0 40.0 40.0 40.0 84-85 39.3562737288015 40.0 40.0 40.0 40.0 40.0 86-87 39.36563556610374 40.0 40.0 40.0 40.0 40.0 88-89 39.398509127810954 40.0 40.0 40.0 40.0 40.0 90-91 39.47568425620053 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10074754516423923 1101 2 -0.010538740315503503 1101 3 -0.02604132868994924 1101 4 -0.11693144761208174 1101 5 -0.02183883805314224 1101 6 0.057721900819402094 1101 7 -0.010318176106231647 1101 8 0.03723296923863728 1101 9 -0.050156706207985735 1101 10-11 -0.07736708339017895 1101 12-13 -0.10402628432019156 1101 14-15 -0.09655915807600479 1101 16-17 0.075745992220412 1101 18-19 -0.05434850089351073 1101 20-21 -0.023110022563294308 1101 22-23 0.05529174217925714 1101 24-25 0.0458399371299123 1101 26-27 0.07831547368424907 1101 28-29 0.04182815159824571 1101 30-31 0.023451403814398475 1101 32-33 0.002379049400097699 1101 34-35 -0.033270232050426785 1101 36-37 -0.01962652700062506 1101 38-39 0.0240044399959487 1101 40-41 -0.06296893256943292 1101 42-43 -0.027788638680831923 1101 44-45 -0.00976700698112154 1101 46-47 -0.15174222678007965 1101 48-49 0.06403797760909669 1101 50-51 -0.031246834505253673 1101 52-53 0.02679391134130782 1101 54-55 -0.018837255750014492 1101 56-57 0.09812631497151614 1101 58-59 -0.08594164548156158 1101 60-61 -0.011158081248332508 1101 62-63 -0.22071383495632801 1101 64-65 0.006361462999038281 1101 66-67 -0.08389401649884576 1101 68-69 -0.037828630634386684 1101 70-71 -0.22298580044615335 1101 72-73 -0.002295645997030249 1101 74-75 -0.10071348837779226 1101 76-77 -0.022987156490870575 1101 78-79 -0.272545756108201 1101 80-81 0.02262678574075494 1101 82-83 0.002409800713891741 1101 84-85 0.38445917961263376 1101 86-87 0.45104520867079145 1101 88-89 0.5754945594202638 1101 90-91 -0.3398263649678128 1102 1 0.18419160271028545 1102 2 0.009754062501585281 1102 3 0.1106027131474363 1102 4 -0.040377926909378914 1102 5 -0.022728995270618668 1102 6 0.027872180302587424 1102 7 -0.006739547733779716 1102 8 0.10166791576374834 1102 9 0.05792539770477845 1102 10-11 -0.019944579430294596 1102 12-13 -0.07787300710166534 1102 14-15 -0.1420700821377494 1102 16-17 -0.0376893273853014 1102 18-19 -0.05369642800533825 1102 20-21 -0.15516255492967446 1102 22-23 -0.0034807244725740816 1102 24-25 -0.06370994440062816 1102 26-27 -0.0033057251480741456 1102 28-29 -0.001306838125671561 1102 30-31 0.01749029583047701 1102 32-33 -0.07527951142471778 1102 34-35 0.010129674432441504 1102 36-37 -0.01724563543444191 1102 38-39 -0.11418895977330124 1102 40-41 -0.01627528895270558 1102 42-43 -0.03679969019365359 1102 44-45 -0.08887162163960483 1102 46-47 -0.0259797125732959 1102 48-49 0.005432124147588979 1102 50-51 -0.030245512990070722 1102 52-53 0.05203393805332013 1102 54-55 -0.24187139535012392 1102 56-57 0.02855554071702926 1102 58-59 -0.04265221890936033 1102 60-61 -0.23048339594158307 1102 62-63 0.0048038713178044645 1102 64-65 0.1532302077716139 1102 66-67 -0.04593439002800892 1102 68-69 0.04592278413482376 1102 70-71 0.034308402006637095 1102 72-73 -0.11922498401014536 1102 74-75 -0.11932450418389351 1102 76-77 -0.02666397295347167 1102 78-79 0.003762028955620167 1102 80-81 0.16682644388924928 1102 82-83 -0.08826781293005581 1102 84-85 0.14583665324049377 1102 86-87 0.011255846495018318 1102 88-89 -0.5321977482720399 1102 90-91 0.23454155133558174 1103 1 0.06089015447141577 1103 2 -0.05358244231310749 1103 3 0.03848769692818621 1103 4 -0.10765590192917784 1103 5 0.07867326776225525 1103 6 0.09183606372829445 1103 7 0.05746379317705674 1103 8 0.0408712566745848 1103 9 0.06480900221040997 1103 10-11 -0.02970327014062235 1103 12-13 -0.006173050523294421 1103 14-15 0.01155626201693849 1103 16-17 0.0068545539970372715 1103 18-19 -0.05449621600564569 1103 20-21 -0.10733172046823114 1103 22-23 0.014403556899551972 1103 24-25 -0.009033818631181134 1103 26-27 -0.027184425564414028 1103 28-29 -0.03269492527542184 1103 30-31 -0.037773298969149494 1103 32-33 -0.07882974196596138 1103 34-35 -0.0035795145873223078 1103 36-37 0.027470338325208843 1103 38-39 0.06484718894310504 1103 40-41 0.002967681269268496 1103 42-43 0.09702084440007752 1103 44-45 0.13099320269524384 1103 46-47 0.07275202218890797 1103 48-49 0.20988764896610945 1103 50-51 -0.12342626888234065 1103 52-53 0.07629253133729463 1103 54-55 -0.026654883016654196 1103 56-57 -0.09828041772838247 1103 58-59 0.07174249078227746 1103 60-61 0.07267922397885229 1103 62-63 0.2483142063455972 1103 64-65 0.14857993836868388 1103 66-67 0.01826572431023976 1103 68-69 -0.017933561246735508 1103 70-71 0.09373798689949098 1103 72-73 0.17264622127849094 1103 74-75 -0.06833118571348251 1103 76-77 0.17070713338379306 1103 78-79 0.03257760829738743 1103 80-81 0.33559220895780584 1103 82-83 0.21915775924564684 1103 84-85 0.33182298627234275 1103 86-87 0.41101982616095967 1103 88-89 0.5754945594202638 1103 90-91 0.4926060674646138 1104 1 -0.018448148842509227 1104 2 -0.08819402977880486 1104 3 -0.1456306497629427 1104 4 -0.024051396297132044 1104 5 -0.08058170157052302 1104 6 0.06855541011624666 1104 7 -0.08000929041345728 1104 8 -0.14380679586002998 1104 9 -0.003565285525034767 1104 10-11 -0.0014552271676464557 1104 12-13 0.017535156163638987 1104 14-15 -0.07816147521671724 1104 16-17 -0.05380512152904515 1104 18-19 -0.06027362410117121 1104 20-21 -0.02310842893145093 1104 22-23 -0.034281523052875684 1104 24-25 -0.07469042354881594 1104 26-27 0.03779897760259132 1104 28-29 -0.027282880716619218 1104 30-31 0.04355506956604671 1104 32-33 -0.10757932373217471 1104 34-35 0.016348897701661258 1104 36-37 0.06550214726627956 1104 38-39 -0.023360140729245416 1104 40-41 -0.03786707146499424 1104 42-43 0.03368019061325356 1104 44-45 -0.03372994561059528 1104 46-47 0.03203556148854858 1104 48-49 -0.12730550559076192 1104 50-51 -0.04562272034264225 1104 52-53 -0.08522875184075218 1104 54-55 0.02790108559135973 1104 56-57 -0.029698740698066217 1104 58-59 -0.18108277600398992 1104 60-61 -0.02452753008664388 1104 62-63 0.020581548046259 1104 64-65 0.027851694125921256 1104 66-67 -0.23504342833678038 1104 68-69 0.11188058134347045 1104 70-71 0.006001358897719911 1104 72-73 0.06867367486032805 1104 74-75 -0.01926070662731405 1104 76-77 0.05406001184861964 1104 78-79 -0.11465959039039575 1104 80-81 -0.08622175843920132 1104 82-83 0.17457123800272 1104 84-85 0.06354998960864577 1104 86-87 -0.1968298620151927 1104 88-89 -0.39441284798714094 1104 90-91 0.4926060674646138 1201 1 0.05950351786935926 1201 2 0.017189897097431128 1201 3 0.005520724019909551 1201 4 -0.15231090970948458 1201 5 -0.006687644232385992 1201 6 0.006194076746751875 1201 7 0.11908479947235406 1201 8 -0.14953518556411893 1201 9 -0.008512531178567428 1201 10-11 0.06581537957514882 1201 12-13 -0.04023153129737267 1201 14-15 -0.11997980497883987 1201 16-17 -0.016451247000311753 1201 18-19 -0.01782061479795516 1201 20-21 -0.04671633801496 1201 22-23 0.025844870394202246 1201 24-25 -0.029856009813698847 1201 26-27 0.16168625417640214 1201 28-29 0.12875064338221875 1201 30-31 0.03514692179963674 1201 32-33 0.09789689247035938 1201 34-35 -0.03363158017751289 1201 36-37 0.014665083886285402 1201 38-39 0.09704766336137283 1201 40-41 0.028709909746645224 1201 42-43 0.11264430008505855 1201 44-45 0.07853451514819909 1201 46-47 -0.06501505851900191 1201 48-49 0.05534240736417928 1201 50-51 0.04123473840501646 1201 52-53 0.006344141266644954 1201 54-55 0.11376726449152841 1201 56-57 0.07226904390524425 1201 58-59 -0.1300781854799382 1201 60-61 -0.07043452350549018 1201 62-63 -0.13934613908296 1201 64-65 -0.009128552400724743 1201 66-67 0.11241921639690133 1201 68-69 -0.026412059122264964 1201 70-71 0.15819430837241555 1201 72-73 0.011417510582163004 1201 74-75 0.24709018594319332 1201 76-77 0.1603028735863674 1201 78-79 -0.027742846387290854 1201 80-81 0.12309695644652408 1201 82-83 -0.024504812579067448 1201 84-85 0.2941948702741968 1201 86-87 0.35825609752004084 1201 88-89 -0.07905089512519226 1201 90-91 0.21674399849909776 1202 1 -0.03644852951521216 1202 2 -0.03689100265393108 1202 3 0.14895097928315693 1202 4 0.13054522659216872 1202 5 -0.003482272191142499 1202 6 0.05715387421842166 1202 7 -0.08227623520426164 1202 8 -0.07385313693500706 1202 9 -0.08247485245156838 1202 10-11 -0.08810021879673258 1202 12-13 -0.04201992290363421 1202 14-15 0.09450156340602689 1202 16-17 -0.08412220635302248 1202 18-19 -0.07165806857150869 1202 20-21 0.011888599490816887 1202 22-23 -0.03533497649232942 1202 24-25 -0.03997540368003172 1202 26-27 -0.06561348127115707 1202 28-29 -0.09488413771342152 1202 30-31 -0.06868122718774572 1202 32-33 0.0032576743617553916 1202 34-35 -0.06798683274733719 1202 36-37 -0.1677493903207008 1202 38-39 -0.087170153924653 1202 40-41 -0.1306831641726589 1202 42-43 -0.22326527736430535 1202 44-45 -0.2487957238275058 1202 46-47 -0.25185154203139604 1202 48-49 -0.2714444381824208 1202 50-51 -0.1144748969421272 1202 52-53 -0.19687613972273965 1202 54-55 -0.28238733062423194 1202 56-57 -0.08364057178894058 1202 58-59 -0.02333109648292009 1202 60-61 -0.10936962616520418 1202 62-63 -0.021995362026714815 1202 64-65 0.09258261498372633 1202 66-67 0.019074521088469965 1202 68-69 0.08545129074050095 1202 70-71 0.09578721100241694 1202 72-73 -0.3428733097218952 1202 74-75 0.0054324879507419155 1202 76-77 -0.023751796177840845 1202 78-79 0.19611053843512138 1202 80-81 -0.0301754859879253 1202 82-83 0.21683794014203528 1202 84-85 0.15460690017775391 1202 86-87 -0.09178103605790255 1202 88-89 -0.11962739179924853 1202 90-91 0.4926060674646138 1203 1 -0.028653315207982644 1203 2 0.19104179764454443 1203 3 0.09470405539583027 1203 4 0.0497872345097079 1203 5 -0.028866413563115145 1203 6 0.06044291030607951 1203 7 0.043476259860653954 1203 8 -0.07091578764426743 1203 9 0.022473074966391948 1203 10-11 -0.0042236822694761145 1203 12-13 -0.03192345368616856 1203 14-15 -0.0031610955602303648 1203 16-17 0.01178737038426192 1203 18-19 -0.023193334347340056 1203 20-21 -0.036387294214137 1203 22-23 0.002048565483015352 1203 24-25 0.044711541553162704 1203 26-27 0.01008557269135224 1203 28-29 0.04386930078955231 1203 30-31 0.011109626203207768 1203 32-33 -0.06148169799435976 1203 34-35 -0.09191010638274832 1203 36-37 -0.03448954250092129 1203 38-39 0.12015114707060093 1203 40-41 -0.08362695697625355 1203 42-43 -0.21810269848381836 1203 44-45 -0.14525106973423618 1203 46-47 -0.04627647533718715 1203 48-49 -0.08620715057674744 1203 50-51 -0.10102668051576558 1203 52-53 -0.08381898301233548 1203 54-55 -0.06737487699690803 1203 56-57 0.03368759425435286 1203 58-59 -0.0028436901446013962 1203 60-61 0.021934494782279046 1203 62-63 -0.016118201239265773 1203 64-65 -0.17858979930637275 1203 66-67 -0.10109581498197429 1203 68-69 0.015968389282001283 1203 70-71 -0.10071240146852034 1203 72-73 0.11010394439063731 1203 74-75 -0.05089793269752363 1203 76-77 0.05641111690028566 1203 78-79 -0.02687372449390324 1203 80-81 -0.13399299064342074 1203 82-83 0.2410461643502586 1203 84-85 0.18794505116296278 1203 86-87 0.24624713295618506 1203 88-89 0.37036635429205944 1203 90-91 0.15927273413128518 1204 1 0.024949844468522997 1204 2 -0.013816581140488893 1204 3 -0.24149304823404094 1204 4 -0.13235908860482937 1204 5 -0.06588127156575041 1204 6 -0.06406188563148163 1204 7 0.02977379606527819 1204 8 -0.1172802788987326 1204 9 -0.025459217679838275 1204 10-11 -0.08580878665164704 1204 12-13 -0.04778352515504025 1204 14-15 -0.06413758606238673 1204 16-17 -0.06806216882957727 1204 18-19 -0.08952923805243529 1204 20-21 -0.15588625979623316 1204 22-23 -0.12551133671747294 1204 24-25 -0.01385908097834232 1204 26-27 -0.006936808350587853 1204 28-29 -0.1280148444051079 1204 30-31 -0.1069351358806685 1204 32-33 -0.1474017930943461 1204 34-35 -0.06526005480723995 1204 36-37 -0.04536263732757817 1204 38-39 -0.1729892776982922 1204 40-41 -0.12425231987091223 1204 42-43 0.041099619817309474 1204 44-45 -0.09401280847117732 1204 46-47 -0.11592879487566421 1204 48-49 -0.039262933762906016 1204 50-51 -0.14164766943876117 1204 52-53 -0.09015277876944339 1204 54-55 -0.04416504706411217 1204 56-57 -0.015278703620502654 1204 58-59 -0.15258247281272475 1204 60-61 -0.14232805210502875 1204 62-63 -0.12632159304914126 1204 64-65 -0.24619857501631515 1204 66-67 -0.3097201867165609 1204 68-69 -0.31109412991821017 1204 70-71 -0.05759208909605462 1204 72-73 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0.19781637458071089 2401 40-41 0.02106081911400537 2401 42-43 0.09833774619693969 2401 44-45 0.16694076808497726 2401 46-47 0.10135175230984572 2401 48-49 -0.013053849236300152 2401 50-51 0.19167054167115793 2401 52-53 0.12137395112660698 2401 54-55 -0.038606533135720156 2401 56-57 0.15675961782279302 2401 58-59 0.17762211457648647 2401 60-61 0.1378649888370802 2401 62-63 0.15446794416639875 2401 64-65 0.15762962256540192 2401 66-67 0.12996914193627873 2401 68-69 0.06606024007771794 2401 70-71 0.06942728948746435 2401 72-73 0.04943952147242925 2401 74-75 0.013622418255188506 2401 76-77 0.1541380172511353 2401 78-79 0.36341206010914107 2401 80-81 0.1567778151373247 2401 82-83 0.24211608780429117 2401 84-85 -0.1831113677730727 2401 86-87 -0.12608430695766515 2401 88-89 0.442161226086931 2401 90-91 0.4926060674646138 2402 1 0.007286391992629149 2402 2 0.0724103455614653 2402 3 0.11696704021662185 2402 4 0.08603342933906077 2402 5 0.055979962393507776 2402 6 0.03181051341151431 2402 7 0.017493943966385928 2402 8 -0.053764002526655474 2402 9 0.12060211778018015 2402 10-11 0.055404292446446846 2402 12-13 0.0639334327221448 2402 14-15 0.05391127930410278 2402 16-17 0.06470372691464377 2402 18-19 0.12575619658832693 2402 20-21 -0.011269135167800926 2402 22-23 0.08286566611005242 2402 24-25 0.029980547818880154 2402 26-27 0.02428424580732269 2402 28-29 0.05361583782501356 2402 30-31 0.11255127026736744 2402 32-33 0.014143722010352633 2402 34-35 0.13812006892842277 2402 36-37 0.08535326153907619 2402 38-39 -0.31961623353532786 2402 40-41 0.027715434867630506 2402 42-43 0.08743565965070843 2402 44-45 0.07850991203368807 2402 46-47 0.11901781059187755 2402 48-49 0.1220855558663203 2402 50-51 0.11180278646785524 2402 52-53 0.11454194013055741 2402 54-55 0.12487715935595389 2402 56-57 0.21233337072001035 2402 58-59 0.10956057330822233 2402 60-61 0.2188529722578636 2402 62-63 0.1018493608918476 2402 64-65 0.12885788562711298 2402 66-67 0.3022600012131136 2402 68-69 0.14027518443977272 2402 70-71 -0.0629010380518693 2402 72-73 0.09419291903724769 2402 74-75 0.045074699725418554 2402 76-77 0.05922836604893433 2402 78-79 0.2806039783675729 2402 80-81 0.25983269205298853 2402 82-83 0.29417520585139556 2402 84-85 0.24055314500250802 2402 86-87 0.23518485865932348 2402 88-89 0.2974196931101005 2402 90-91 0.4926060674646138 2403 1 6.30071862374848E-4 2403 2 -0.05865864900334117 2403 3 0.044795813020179764 2403 4 0.06463220647422929 2403 5 0.05238058666839862 2403 6 -0.05413045909035219 2403 7 -0.011321958065117599 2403 8 0.07185455855019285 2403 9 -0.012975295135866816 2403 10-11 0.011589685545907003 2403 12-13 0.011398567579014696 2403 14-15 0.0812779406741555 2403 16-17 0.05002630159146548 2403 18-19 -0.0025340346415276827 2403 20-21 0.046514166567241944 2403 22-23 -0.023746902537332915 2403 24-25 0.1366188014164038 2403 26-27 0.046233559387914624 2403 28-29 0.010357157726708976 2403 30-31 0.044375576501629155 2403 32-33 0.0718403416998612 2403 34-35 0.10848263550196435 2403 36-37 0.014523497254366191 2403 38-39 0.08661695009728021 2403 40-41 -0.025175645075833586 2403 42-43 -0.004386346665171459 2403 44-45 0.0710666545569083 2403 46-47 -0.05292211542894876 2403 48-49 -0.009510291443973529 2403 50-51 0.0733068158075767 2403 52-53 -0.06326204236787447 2403 54-55 -0.0720873906438797 2403 56-57 -0.020936310929691615 2403 58-59 -0.12989580898972974 2403 60-61 -0.03362457478061742 2403 62-63 0.10914485604857305 2403 64-65 0.029412654100276825 2403 66-67 -3.6687627158471514E-6 2403 68-69 -0.06832575938220486 2403 70-71 -0.09401597251189742 2403 72-73 0.023837533375292708 2403 74-75 0.03114170410481165 2403 76-77 0.012667586315068036 2403 78-79 -0.0954783038406859 2403 80-81 -0.06696898220638303 2403 82-83 -0.31869502322314247 2403 84-85 -0.47290076276297555 2403 86-87 0.03560063391493884 2403 88-89 -0.3272203727064351 2403 90-91 0.264034638893186 2404 1 -0.034091740236583234 2404 2 0.07613724430728297 2404 3 -0.010849434333358943 2404 4 0.0509598194947003 2404 5 0.03363379210544082 2404 6 0.03025954170744427 2404 7 0.08736209022961816 2404 8 0.14434514816411337 2404 9 0.07519771713052847 2404 10-11 0.11712899158627721 2404 12-13 0.17041601240923399 2404 14-15 0.09555706831866928 2404 16-17 0.07112960395573964 2404 18-19 -0.029272896816280536 2404 20-21 0.058485690371142596 2404 22-23 0.11102163771052886 2404 24-25 0.07750717501779292 2404 26-27 0.004342758202199093 2404 28-29 0.07812271296798201 2404 30-31 0.0038859981221506246 2404 32-33 -0.04202088195740572 2404 34-35 0.06918451752199672 2404 36-37 0.15440097760480143 2404 38-39 0.10237955059162829 2404 40-41 0.10430459130490277 2404 42-43 0.17619123401775028 2404 44-45 0.15876475580274274 2404 46-47 0.1391043656536155 2404 48-49 0.13387239054544864 2404 50-51 0.04920975614363243 2404 52-53 0.17395715275065982 2404 54-55 0.1349432129992607 2404 56-57 -0.05134665748265377 2404 58-59 0.09327525058098729 2404 60-61 0.060327133389563414 2404 62-63 0.03918949428459939 2404 64-65 0.06033819227067028 2404 66-67 0.16978459373191868 2404 68-69 0.1098630400298859 2404 70-71 0.10330592392181615 2404 72-73 0.025943354303031185 2404 74-75 0.12722254274413558 2404 76-77 0.08417399512220669 2404 78-79 0.03806424506030481 2404 80-81 4.313642720177313E-4 2404 82-83 -0.0489971256930275 2404 84-85 -0.40330065404494064 2404 86-87 -0.3497826429748443 2404 88-89 0.031535040128673586 2404 90-91 0.07155343588566865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 14.0 31 408.0 32 2412.0 33 4272.0 34 5673.0 35 7101.0 36 10233.0 37 14943.0 38 38294.0 39 243970.0 40 254007.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.976118432482924 13.638967397007193 26.433659541015643 30.951254629494247 2 27.9553556681165 18.538374717832955 27.402721191433134 26.103548422617408 3 28.155237929771026 19.62303488398096 21.185666586963965 31.036060599284056 4 27.747894042423628 21.08090627134126 20.937613425834343 30.233586260400774 5 27.909765071981862 21.312789531537327 21.7545374634242 29.022907933056608 6 27.82341092018778 23.990112277599355 23.4879852475457 24.698491554667168 7 26.5086603581117 24.231800690489173 22.831040017064407 26.42849893433472 8 25.71874349548533 24.547801839584263 23.136031871906862 26.597422793023544 9 25.44161891671985 24.133061082660877 23.932657523218428 26.49266247740084 10-11 25.382271939889257 22.967882104220173 23.685378453090948 27.96446750279963 12-13 25.633593485250127 23.030497465282018 23.540967476136494 27.79494157333136 14-15 25.935747006418076 22.800248397201575 23.493661914894716 27.770342681485637 16-17 26.178037490087334 22.615756708358635 23.095177069016234 28.111028732537797 18-19 26.06218187010065 22.983277914151586 23.131129295559987 27.82341092018778 20-21 26.06639636555673 22.939412757363755 23.157362379521338 27.836828497558173 22-23 26.039389190593248 22.364263142775066 23.25369370423187 28.342653962399822 24-25 26.108541320117595 22.70469116349318 23.222558043923645 27.964209472465583 26-27 26.271960531680104 23.004436401543366 23.119345910305217 27.604257156471313 28-29 26.36872190694738 22.687833181668836 23.142310610035317 27.80113430134847 30-31 26.250407477458648 22.87653712538952 23.213872553952598 27.659182843199233 32-33 26.422279845068108 22.89179266663915 23.252603784784817 27.433323703507924 34-35 26.460730335703474 22.546773560393355 23.240047614428295 27.752448489474872 36-37 26.539580064935308 22.87192018148779 23.308051451638995 27.280448301937906 38-39 26.66549565975118 22.888900228248218 23.196632995311393 27.2489711166892 40-41 26.59775759450673 22.651502148569385 23.26015213166244 27.49058812526144 42-43 26.540205346278263 22.786529942362737 23.371238529925044 27.30202618143396 44-45 26.718703126538596 22.94672770283113 23.26137386330067 27.073195307329605 46-47 26.704045975332093 22.586599456965093 23.269532819520037 27.43982174818278 48-49 26.5895307492801 22.9548689881111 23.263109314162026 27.192490948446775 50-51 26.837185436373375 22.947667544172763 23.208270123148314 27.00687689630555 52-53 26.919607011719275 22.629905963088934 23.269399059931615 27.181087965260176 54-55 26.87801527413359 22.833234587513754 23.231527478465814 27.057222659886843 56-57 27.031735868146328 22.922629007665993 23.09791741103804 26.94771771314964 58-59 26.97145285278325 22.728203345308458 23.10020473596786 27.200139065940434 60-61 27.24092170346242 22.880672546367727 22.917076743009655 26.961329007160195 62-63 27.1396664750931 22.796319918511145 23.079474994977033 26.984538611418717 64-65 27.309299407965 22.652596762989 22.888477609464267 27.14962621958174 66-67 27.21829762594094 22.79183555298205 22.85871453387377 27.131152287203243 68-69 27.288707750502127 22.91165488796945 22.730986708681513 27.06865065284691 70-71 27.480333537309143 22.646844150195005 22.529619499104225 27.343202813391628 72-73 27.365567141586165 22.905343582261988 22.58109401550832 27.147995260643526 74-75 27.611918505611744 22.831959194400913 22.43772571083704 27.118396589150308 76-77 27.517765253614314 22.70818255899432 22.473401981958364 27.300650205433 78-79 27.62092807078253 22.81433834754819 22.296632537436984 27.268101044232296 80-81 28.03129828388238 22.868072941195912 21.70388915053417 27.396739624387546 82-83 28.20934175082424 22.120874892094562 21.766217017337674 27.903566339743524 84-85 28.135881336879336 22.162969868121625 21.605022893647355 28.096125901351687 86-87 29.318986704855533 21.82958218024887 20.65295221876028 28.198478896135313 88-89 28.66040658888994 21.03765531632363 20.006955641974137 30.29498245281229 90-91 24.338015196868522 24.04635812418451 22.004758615396426 29.610868063550537 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 2.0 7 3.5 8 4.0 9 2.5 10 3.0 11 9.5 12 14.5 13 19.0 14 35.0 15 59.5 16 82.0 17 108.5 18 149.5 19 204.5 20 284.0 21 397.5 22 536.0 23 711.0 24 935.0 25 1171.5 26 1465.0 27 1876.5 28 2336.0 29 2921.5 30 3598.0 31 4244.0 32 4932.5 33 5690.0 34 6837.0 35 8184.5 36 9537.0 37 10992.0 38 12544.0 39 14299.0 40 16114.0 41 18194.0 42 20389.0 43 22495.0 44 24551.5 45 26299.0 46 28137.5 47 30109.5 48 31759.5 49 33338.5 50 34876.0 51 36200.5 52 37366.0 53 38524.0 54 39625.5 55 40160.0 56 40416.0 57 40605.0 58 40498.5 59 40082.5 60 39267.0 61 38100.5 62 36658.0 63 34660.5 64 32061.5 65 29462.5 66 26517.5 67 23502.0 68 20706.0 69 17878.0 70 15214.5 71 12654.0 72 10284.0 73 8129.5 74 6282.0 75 4906.5 76 3730.0 77 2670.0 78 1953.0 79 1436.5 80 1008.0 81 682.5 82 472.5 83 329.5 84 228.0 85 156.5 86 94.0 87 56.0 88 42.0 89 30.0 90 17.5 91 10.0 92 5.5 93 2.0 94 2.5 95 4.5 96 4.5 97 3.0 98 2.5 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.019094244719409213 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.06501727419736028 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.895185595872623E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0010400416016640665 84-85 0.0013708582943781102 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 7719.0 32-33 17404.0 34-35 19337.0 36-37 21337.0 38-39 23044.0 40-41 24373.0 42-43 24994.0 44-45 25989.0 46-47 25986.0 48-49 25862.0 50-51 26367.0 52-53 25591.0 54-55 24660.0 56-57 23935.0 58-59 22886.0 60-61 22386.0 62-63 21347.0 64-65 20437.0 66-67 19462.0 68-69 18172.0 70-71 17383.0 72-73 16044.0 74-75 15214.0 76-77 14307.0 78-79 13359.0 80-81 12679.0 82-83 11811.0 84-85 10845.0 86-87 10205.0 88-89 9380.0 90-91 8812.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.85885742563684 #Duplication Level Percentage of deduplicated Percentage of total 1 99.86814437750823 99.7271879075651 2 0.1237814914875044 0.24721356620766763 3 0.007170072053530883 0.02147985608775251 4 6.499310757366565E-4 0.0025960549851391022 5 0.0 0.0 6 2.5412787501459874E-4 0.0015226151543377723 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0025803033404607047 0.0 0.0 0.0 0.0 2 0.0025803033404607047 0.0 0.0 0.0 0.0 3 0.0025803033404607047 0.0 0.0 0.0 0.0 4 0.0025803033404607047 0.0 0.0 0.0 0.0 5 0.0025803033404607047 0.0 0.0 0.0 0.0 6 0.002752323563158085 0.0 0.0 0.0 0.0 7 0.0034404044539476063 0.0 0.0 0.0 0.0 8 0.019610305387501355 0.0 0.0 0.0 0.0 9 0.020814446946383017 0.0 0.0 0.0 1.720202226973803E-4 10-11 0.020814446946383017 0.0 0.0 0.0 1.720202226973803E-4 12-13 0.020814446946383017 0.0 0.0 0.0 1.720202226973803E-4 14-15 0.020814446946383017 0.0 0.0 0.0 1.720202226973803E-4 16-17 0.020814446946383017 0.0 0.0 0.0 1.720202226973803E-4 18-19 0.020814446946383017 0.0 0.0 0.0 1.720202226973803E-4 20-21 0.021158487391777777 0.0 0.0 0.0 1.720202226973803E-4 22-23 0.021330507614475157 0.0 0.0 0.0 3.440404453947606E-4 24-25 0.021330507614475157 0.0 0.0 0.0 5.160606680921409E-4 26-27 0.02141651772582385 0.0 0.0 0.0 5.160606680921409E-4 28-29 0.021502527837172537 0.0 0.0 0.0 5.160606680921409E-4 30-31 0.021760558171218608 0.0 0.0 0.0 5.160606680921409E-4 32-33 0.02201858850526468 0.0 0.0 0.0 6.880808907895212E-4 34-35 0.02219060872796206 0.0 0.0 0.0 6.880808907895212E-4 36-37 0.02236262895065944 0.0 0.0 0.0 6.880808907895212E-4 38-39 0.02253464917335682 0.0 0.0 0.0 8.601011134869016E-4 40-41 0.0227066693960542 0.0 0.0 0.0 0.001118131447532972 42-43 0.0227066693960542 0.0 0.0 0.0 0.001204141558881662 44-45 0.02287868961875158 0.0 0.0 0.0 0.001204141558881662 46-47 0.02305070984144896 0.0 0.0 0.0 0.0013761617815790425 48-49 0.02322273006414634 0.0 0.0 0.0 0.0013761617815790425 50-51 0.02322273006414634 0.0 0.0 0.0 0.0013761617815790425 52-53 0.02322273006414634 0.0 0.0 0.0 0.0013761617815790425 54-55 0.02373879073223848 0.0 0.0 0.0 0.0013761617815790425 56-57 0.02391081095493586 0.0 0.0 0.0 0.0013761617815790425 58-59 0.02391081095493586 0.0 0.0 0.0 0.0013761617815790425 60-61 0.024082831177633244 0.0 0.0 0.0 0.0014621718929277326 62-63 0.024082831177633244 0.0 0.0 0.0 0.0015481820042764227 64-65 0.024168841288981932 0.0 0.0 0.0 0.0015481820042764227 66-67 0.024254851400330624 0.0 0.0 0.0 0.0015481820042764227 68-69 0.024426871623028003 0.0 0.0 0.0 0.0015481820042764227 70-71 0.024426871623028003 0.0 0.0 0.0 0.0015481820042764227 72-73 0.024426871623028003 0.0 0.0 0.0 0.0015481820042764227 74-75 0.024426871623028003 0.0 0.0 0.0 0.0015481820042764227 76-77 0.024426871623028003 0.0 0.0 0.0 0.0015481820042764227 78-79 0.024426871623028003 0.0 0.0 0.0 0.0015481820042764227 80 0.024426871623028003 0.0 0.0 0.0 0.0015481820042764227 >>END_MODULE