##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-25B_S14_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	852037
Sequences flagged as poor quality	0
Sequence length	61
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.44790191036305	40.0	40.0	40.0	40.0	40.0
2	39.15187485989458	40.0	40.0	40.0	40.0	40.0
3	39.11051984831644	40.0	40.0	40.0	40.0	40.0
4	39.037384526728296	40.0	40.0	40.0	40.0	40.0
5	39.089091201438436	40.0	40.0	40.0	40.0	40.0
6	39.15775019159966	40.0	40.0	40.0	40.0	40.0
7	39.220207573145295	40.0	40.0	40.0	40.0	40.0
8	39.22633406765199	40.0	40.0	40.0	40.0	40.0
9	39.23327742809291	40.0	40.0	40.0	40.0	40.0
10	39.237948586739776	40.0	40.0	40.0	40.0	40.0
11	39.24143669817156	40.0	40.0	40.0	40.0	40.0
12	39.247985709540785	40.0	40.0	40.0	40.0	40.0
13	39.0889832249069	40.0	40.0	40.0	40.0	40.0
14	39.24053063423302	40.0	40.0	40.0	40.0	40.0
15	39.155529630755474	40.0	40.0	40.0	40.0	40.0
16	39.338610881921795	40.0	40.0	40.0	40.0	40.0
17	39.28649577424454	40.0	40.0	40.0	40.0	40.0
18	39.368956981915105	40.0	40.0	40.0	40.0	40.0
19	39.32517484569332	40.0	40.0	40.0	40.0	40.0
20	39.34237597663012	40.0	40.0	40.0	40.0	40.0
21	39.31883709275536	40.0	40.0	40.0	40.0	40.0
22	39.37802231593229	40.0	40.0	40.0	40.0	40.0
23	39.370529683570076	40.0	40.0	40.0	40.0	40.0
24	39.36954381089084	40.0	40.0	40.0	40.0	40.0
25	39.335554676616155	40.0	40.0	40.0	40.0	40.0
26	39.305039569877835	40.0	40.0	40.0	40.0	40.0
27	39.32055532799632	40.0	40.0	40.0	40.0	40.0
28	39.3304938635294	40.0	40.0	40.0	40.0	40.0
29	39.33348434398976	40.0	40.0	40.0	40.0	40.0
30	39.336108643169254	40.0	40.0	40.0	40.0	40.0
31	39.286599056144276	40.0	40.0	40.0	40.0	40.0
32	39.3140110112589	40.0	40.0	40.0	40.0	40.0
33	39.31728316962761	40.0	40.0	40.0	40.0	40.0
34	39.304922204082686	40.0	40.0	40.0	40.0	40.0
35	39.31051351056351	40.0	40.0	40.0	40.0	40.0
36	39.27827547395242	40.0	40.0	40.0	40.0	40.0
37	39.301560847709666	40.0	40.0	40.0	40.0	40.0
38	39.275946936576695	40.0	40.0	40.0	40.0	40.0
39	38.05697170428045	40.0	40.0	40.0	40.0	40.0
40	39.275909379522254	40.0	40.0	40.0	40.0	40.0
41	39.292195057256905	40.0	40.0	40.0	40.0	40.0
42	39.24071841950526	40.0	40.0	40.0	40.0	40.0
43	39.280251913942706	40.0	40.0	40.0	40.0	40.0
44	39.22782696056627	40.0	40.0	40.0	40.0	40.0
45	39.02208002704108	40.0	40.0	40.0	40.0	40.0
46	39.07314823182561	40.0	40.0	40.0	40.0	40.0
47	39.15355788539699	40.0	40.0	40.0	40.0	40.0
48	39.25621539909652	40.0	40.0	40.0	40.0	40.0
49	39.248469256616794	40.0	40.0	40.0	40.0	40.0
50	39.225151020436904	40.0	40.0	40.0	40.0	40.0
51	39.17648646713699	40.0	40.0	40.0	40.0	40.0
52	39.18199796487711	40.0	40.0	40.0	40.0	40.0
53	39.09346659828153	40.0	40.0	40.0	40.0	40.0
54	38.99660226023048	40.0	40.0	40.0	40.0	40.0
55	39.05678743998207	40.0	40.0	40.0	40.0	40.0
56	39.055707674666714	40.0	40.0	40.0	40.0	40.0
57	38.935299758109096	40.0	40.0	40.0	40.0	40.0
58	38.83310231832655	40.0	40.0	40.0	40.0	40.0
59	38.69532426408712	40.0	40.0	40.0	40.0	40.0
60	38.67847523053576	40.0	40.0	40.0	40.0	40.0
61	38.5339791581821	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.08805747391721752
1101	2	-0.011740757471585539
1101	3	-0.05833620310533405
1101	4	-0.0051272538699791426
1101	5	0.031141020879907444
1101	6	0.018237754184063704
1101	7	-0.0019906784364565056
1101	8	0.02987694916360084
1101	9	-0.0035928762901775713
1101	10	-0.06710134872574969
1101	11	0.02005594383852838
1101	12	-0.011407415299863999
1101	13	0.039727849443515595
1101	14	0.05517826390270386
1101	15	0.20333163254276343
1101	16	0.10910433063369851
1101	17	0.0026151090133339494
1101	18	0.12843240727927707
1101	19	0.030093326318187508
1101	20	0.010449049483561623
1101	21	0.0566642211126549
1101	22	0.004862361565365347
1101	23	0.018830235942751017
1101	24	0.09807740784172125
1101	25	-0.04351055492917766
1101	26	0.015296134281506113
1101	27	0.027604956743566333
1101	28	-0.037902296726272766
1101	29	-0.030568096830151603
1101	30	0.009670172297106205
1101	31	0.028265688022337088
1101	32	-0.04405011272466908
1101	33	-0.02233850290286199
1101	34	-0.12561132245988915
1101	35	-0.014625060261543865
1101	36	-0.09905649594369237
1101	37	-0.08345535852793518
1101	38	-0.1330188786566069
1101	39	1.0493066896945464
1101	40	-0.04517504206808809
1101	41	-0.10643242132963593
1101	42	-0.15730878353490851
1101	43	-0.06738377999259626
1101	44	-0.03884269537969942
1101	45	-0.057649002698852314
1101	46	-0.08016981861546668
1101	47	-0.07501901823383861
1101	48	0.008242312866670432
1101	49	0.028814109919366615
1101	50	0.06568808934240877
1101	51	-0.039514701745588354
1101	52	0.08176762337551935
1101	53	0.07077618086182014
1101	54	-0.09037730534235777
1101	55	-0.03808130389251119
1101	56	-0.011186931467129568
1101	57	-0.11549938427978645
1101	58	-0.07469474378167718
1101	59	0.053842806738821025
1101	60	-0.03391359618638745
1101	61	-0.04440924545542657
1102	1	0.09884709826251736
1102	2	0.03278042416953042
1102	3	0.09710421694435212
1102	4	0.04202244712592318
1102	5	-0.2091714279802872
1102	6	-0.10434376735161521
1102	7	-0.07490361267694823
1102	8	-0.20053750656978053
1102	9	-0.13133196459238405
1102	10	-0.04660585009856533
1102	11	-0.1409345827958859
1102	12	-0.010291832110837618
1102	13	-0.09660967918141239
1102	14	0.031005512155793724
1102	15	0.05223909903516244
1102	16	-0.01804113478269187
1102	17	0.007926696852941006
1102	18	-0.07607549004428904
1102	19	-0.059740534145134916
1102	20	-0.0684386188833983
1102	21	-0.03965991623563525
1102	22	-0.03805496869325253
1102	23	0.010117349406009168
1102	24	-0.24915668386713463
1102	25	-0.1206280505465287
1102	26	-0.04114189309360228
1102	27	-0.05921693606845935
1102	28	-0.05796140231306168
1102	29	-0.053177106849005895
1102	30	-0.015113315788184423
1102	31	-0.04723030796456129
1102	32	-0.06709230701155633
1102	33	0.08037536979658455
1102	34	-0.0516512588406286
1102	35	-0.10205341104881427
1102	36	-0.14778277375706494
1102	37	-0.11541366160076194
1102	38	-0.16349579849184437
1102	39	1.1748830654289577
1102	40	-0.039830296914132646
1102	41	-0.14930803754762678
1102	42	-0.17220176562889122
1102	43	-0.056015099536054436
1102	44	-0.0060394155865139965
1102	45	-0.09471888013444385
1102	46	-0.017205148268693904
1102	47	-0.042875675955158954
1102	48	0.05459516872755188
1102	49	-0.031097948326269886
1102	50	-0.007229123261218717
1102	51	-0.137591898390923
1102	52	0.0069401216294053825
1102	53	-0.08276678513481528
1102	54	-0.057123727828582105
1102	55	-0.19639357520063072
1102	56	-0.16295544635102033
1102	57	-0.06060477640780704
1102	58	-0.17605130577462802
1102	59	-0.016927737158788148
1102	60	-0.04904081866234122
1102	61	0.07364255910466255
1103	1	0.008832892820940685
1103	2	0.17062106652524278
1103	3	0.1877971509880325
1103	4	0.09481099923683445
1103	5	0.012054938885576405
1103	6	0.04863556840803085
1103	7	-0.022354355906522017
1103	8	0.09539904100757468
1103	9	0.03038817733150978
1103	10	0.1295459762687372
1103	11	0.029613381779704184
1103	12	-0.018369137558281068
1103	13	0.09771554058960419
1103	14	-0.006644346551183844
1103	15	0.16053111766539274
1103	16	0.013332659767030464
1103	17	0.04972724201123668
1103	18	-0.02546452553178824
1103	19	-0.014842202033655383
1103	20	0.027884281898224117
1103	21	-0.07145791616166974
1103	22	0.012429821912448347
1103	23	0.0663582806564591
1103	24	0.10981678263043193
1103	25	0.05833875918974485
1103	26	-0.018347924206345567
1103	27	0.031319368297808126
1103	28	-0.05875760317321976
1103	29	0.11828670625698123
1103	30	0.0016025514507589378
1103	31	0.06249095647201841
1103	32	0.15451040616252243
1103	33	-0.025373591987950306
1103	34	0.052135481151928786
1103	35	0.10197632248494415
1103	36	0.09628977188852161
1103	37	0.00482136993183957
1103	38	-0.004755971263080028
1103	39	1.3862591659155967
1103	40	-0.003707577561883113
1103	41	0.022590857995602676
1103	42	-0.0016276961070644802
1103	43	0.08758385887659159
1103	44	0.10078419981801545
1103	45	0.10139670386583077
1103	46	0.10776575559331292
1103	47	0.11581194138113915
1103	48	0.08748522557107208
1103	49	0.024183626150055204
1103	50	0.07013941114183808
1103	51	0.10377008367063212
1103	52	0.10646957997352757
1103	53	0.2057885888946771
1103	54	0.18599816269162517
1103	55	0.09266214363169922
1103	56	0.12304164294747721
1103	57	0.18422046185420982
1103	58	0.07824132632183733
1103	59	0.1489735469870226
1103	60	0.09047706648895115
1103	61	0.2779703241079403
1104	1	-0.08034002461448608
1104	2	-0.07349961875712552
1104	3	-0.10155820072588284
1104	4	-0.10056566332615091
1104	5	-0.13164471072305162
1104	6	-0.013387732237347905
1104	7	0.01021059820733683
1104	8	0.09142815647344804
1104	9	-0.04183010811282628
1104	10	-0.011723030476758822
1104	11	-0.0632561156661069
1104	12	-0.061110571800846
1104	13	-0.09585092981870247
1104	14	-0.01830555434012382
1104	15	-0.016323458485139497
1104	16	-0.12347454442765127
1104	17	-0.21727011399793383
1104	18	0.003242749089494623
1104	19	-0.06620245857369866
1104	20	0.0034744448155166197
1104	21	-0.009138540792257288
1104	22	-0.016354736919886648
1104	23	-0.11449003873850216
1104	24	-0.13745786041992147
1104	25	-0.03249740154835479
1104	26	-0.026905789942993863
1104	27	-0.11445498139909205
1104	28	-0.11319398901518696
1104	29	-0.08326725064019769
1104	30	0.04647202277232765
1104	31	-0.030046545419267545
1104	32	-0.11789357909034237
1104	33	0.03706551948309311
1104	34	-0.016718219836548087
1104	35	-0.043493244407805776
1104	36	-0.03016461355505129
1104	37	-0.07906465436152388
1104	38	-0.11833110621110876
1104	39	1.1270174437621279
1104	40	-0.16899782331478974
1104	41	-0.031687693894213
1104	42	-0.0014034630440136198
1104	43	-0.10239488252281603
1104	44	-0.05390831243044403
1104	45	5.647556614931659E-4
1104	46	-0.04893288061249024
1104	47	0.08360293004212593
1104	48	-0.06415292757575486
1104	49	0.0215494319141456
1104	50	-0.08170555890781372
1104	51	-0.06733498258153503
1104	52	-0.13850721843550673
1104	53	-0.1928662304289901
1104	54	-0.009111814917723393
1104	55	0.021541076282318272
1104	56	-0.10611583941594205
1104	57	-0.01708531475959063
1104	58	0.015129515661627124
1104	59	-0.08146837719390021
1104	60	-0.004226570331859136
1104	61	0.12732380358463047
1201	1	-0.019488226622478066
1201	2	0.027636943597798336
1201	3	0.08385550705518341
1201	4	0.13398743817874958
1201	5	-0.025896800097086725
1201	6	-0.022398103055600416
1201	7	-0.017515153480957224
1201	8	-0.09482623183850336
1201	9	-0.04834268773834083
1201	10	0.0030201348848279963
1201	11	-0.05214997139616884
1201	12	-0.18582175627390995
1201	13	-0.1308534181791856
1201	14	-0.054621336581234914
1201	15	-0.06049152022342241
1201	16	-0.02139544986214048
1201	17	-0.05033251647427761
1201	18	-0.07757575040892561
1201	19	-0.1465052329002816
1201	20	-0.04344522896810332
1201	21	-0.043793978984858484
1201	22	-0.054684380150696654
1201	23	-0.0770926886605352
1201	24	-0.13887516157135593
1201	25	-0.03948582791026212
1201	26	-0.08713192761682365
1201	27	-0.006064316078592924
1201	28	0.009679554040992855
1201	29	-0.16221146367295347
1201	30	-0.11003628608830951
1201	31	0.010466711600756184
1201	32	-0.1802187236025219
1201	33	-0.11614742947601542
1201	34	-0.07872164237632262
1201	35	-0.05579628063870956
1201	36	-0.0813620578862384
1201	37	-0.0262074048796066
1201	38	0.10162487122954644
1201	39	1.2881446454626726
1201	40	0.015119835314486352
1201	41	-0.0464685821001396
1201	42	-0.011849457538090746
1201	43	0.061489320200827535
1201	44	-0.11291815782336556
1201	45	-0.16550144415803203
1201	46	-0.13369557293500378
1201	47	-0.07254916738843775
1201	48	-0.07543906370295161
1201	49	-0.07233626086184586
1201	50	-0.14327301314663288
1201	51	-0.13577500016037902
1201	52	-0.07484668523387938
1201	53	-0.009212562785030798
1201	54	-0.16051568404208894
1201	55	-0.025885161410940327
1201	56	-0.11980545962504152
1201	57	-0.006964275624561367
1201	58	-0.10801185958933246
1201	59	-0.21148770476263934
1201	60	-0.15701702343441326
1201	61	-0.11158359195206202
1202	1	0.010447721797575582
1202	2	-0.01633532933946924
1202	3	-0.12174551250402033
1202	4	-0.04245268056810403
1202	5	-0.004166800937170478
1202	6	0.07203879366969801
1202	7	0.08409836557891026
1202	8	-0.02367261461566983
1202	9	-0.03695426559592363
1202	10	-0.026460871206595016
1202	11	-0.0917389785137317
1202	12	0.1344305694993011
1202	13	0.03806926962752044
1202	14	0.009162427259639117
1202	15	-0.03445894513172476
1202	16	0.007224510188407862
1202	17	-0.039048141818916804
1202	18	-0.12900069003418224
1202	19	-0.1219988645703225
1202	20	0.017241529569133718
1202	21	-0.0835337487877581
1202	22	-0.09886899489828949
1202	23	-0.03824156933386291
1202	24	-0.009626948460869755
1202	25	0.03867666161697514
1202	26	-0.01027056253237646
1202	27	-0.03855951416762338
1202	28	0.023748791538800162
1202	29	-0.0445252621348331
1202	30	0.007575595816199154
1202	31	-1.737365147889136E-4
1202	32	-0.07996790811043297
1202	33	0.06120204307356403
1202	34	-0.13003191742399878
1202	35	-0.015429571640815709
1202	36	-0.18358818306890612
1202	37	-0.11396774423367617
1202	38	-0.23674127267369727
1202	39	1.1711427154083367
1202	40	-0.1789701347068302
1202	41	-0.08468214514130068
1202	42	-0.05881044145571934
1202	43	-0.2957959481481254
1202	44	-0.1786283927384389
1202	45	-0.10557858528345321
1202	46	-0.1350874735016987
1202	47	-0.11200439916549243
1202	48	-0.05680606984666525
1202	49	-0.0813507015648014
1202	50	-0.026924034159051757
1202	51	-0.06596709916050258
1202	52	-0.1332783737454335
1202	53	-0.14042334507747256
1202	54	-0.021974331602592656
1202	55	0.022114704813716912
1202	56	-0.14885116500528994
1202	57	-0.2296588109151756
1202	58	-0.042100961663500414
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2403	7	-0.11307917260406697
2403	8	0.04334589517324616
2403	9	0.1013812569710808
2403	10	-0.003429461994365113
2403	11	0.1349073783274406
2403	12	0.033177254194292516
2403	13	0.0027624945224928865
2403	14	0.09142840433167976
2403	15	0.023216597577338405
2403	16	-0.0056547822333783415
2403	17	-0.0012490513993768104
2403	18	0.03645448883152369
2403	19	0.06573169696392256
2403	20	0.1308820490130529
2403	21	0.04894368644811209
2403	22	0.019350182341284494
2403	23	-0.15645515466113125
2403	24	0.003287573008435629
2403	25	0.05027820207697431
2403	26	0.03688827070394041
2403	27	-0.016458039971780636
2403	28	0.06343347478772898
2403	29	-0.0585704682722934
2403	30	0.08081454885364536
2403	31	0.10559672555051947
2403	32	-0.025751522353480993
2403	33	0.08259669955400284
2403	34	0.1263432819975776
2403	35	-0.04978068893285581
2403	36	0.1388096065600024
2403	37	-0.06674180804969154
2403	38	0.07890596042012987
2403	39	1.1644117305663286
2403	40	-0.13177383565807332
2403	41	0.06827790567799497
2403	42	-0.08718995559682696
2403	43	0.055278286165297175
2403	44	-0.03512091048018107
2403	45	-0.021158603591374003
2403	46	0.06095434729204641
2403	47	-0.025572447425929568
2403	48	0.06178329198752408
2403	49	0.03346356027202546
2403	50	0.10501042639196356
2403	51	-0.044661354085718585
2403	52	-0.004652088514284003
2403	53	0.03692862721795365
2403	54	0.018088364918178
2403	55	0.08702450447227505
2403	56	0.10049108630978765
2403	57	0.054491416927291425
2403	58	0.053378704186187065
2403	59	0.013163453079158671
2403	60	0.02695860955513041
2403	61	0.03431662717855488
2404	1	0.06924623302036537
2404	2	0.15408554295385102
2404	3	-0.10422845487503452
2404	4	-0.0278478908677684
2404	5	0.0892229691925337
2404	6	0.04407645372815239
2404	7	0.03718344496856929
2404	8	0.04459522379133318
2404	9	0.013666165010853604
2404	10	-0.1780263684075436
2404	11	0.02865333093757272
2404	12	-0.017647972606447127
2404	13	-0.053572471282571144
2404	14	0.0067208682827271105
2404	15	0.12877957702042409
2404	16	0.019743140096693423
2404	17	-0.1194569288688001
2404	18	-0.06542044432410421
2404	19	0.023717586484721664
2404	20	0.0073254466697676435
2404	21	-0.06346650703915913
2404	22	-0.11308217703285095
2404	23	-0.028068128404072468
2404	24	0.00412555165819839
2404	25	-0.11885597675683357
2404	26	-0.020263518609660025
2404	27	0.02598387447447692
2404	28	-0.018401663196684126
2404	29	-0.06806795139812749
2404	30	-0.10824777301712629
2404	31	-0.04513953212850197
2404	32	-0.05064524715095331
2404	33	0.008365659555508387
2404	34	0.06879777200001058
2404	35	-0.014907613903801575
2404	36	0.06144172923161051
2404	37	0.018840140503350256
2404	38	0.10281479586486597
2404	39	1.2331671148435959
2404	40	-0.03668735226544584
2404	41	-0.025669725945178357
2404	42	0.024611439937324064
2404	43	-0.023373003064264708
2404	44	0.009239070104101188
2404	45	0.03472387724853121
2404	46	-0.027609300888549626
2404	47	0.057278137337426926
2404	48	-0.004555705047231129
2404	49	0.008610970471387702
2404	50	-0.14956349143441372
2404	51	-0.0383512455715973
2404	52	-0.13624411866741326
2404	53	-0.07001531283307827
2404	54	0.021778761776872102
2404	55	0.009178285895004024
2404	56	-0.1335082813865469
2404	57	0.0828122746037323
2404	58	-0.029624667708269214
2404	59	0.07319939331353709
2404	60	0.10993612516585927
2404	61	0.02287287105972524
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	2.0
17	29.0
18	61.0
19	125.0
20	130.0
21	229.0
22	278.0
23	445.0
24	656.0
25	817.0
26	1136.0
27	1433.0
28	1889.0
29	2278.0
30	2960.0
31	3706.0
32	5033.0
33	6155.0
34	7838.0
35	10142.0
36	15049.0
37	23094.0
38	60609.0
39	361701.0
40	346241.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	29.403652658276574	15.38712520700392	24.062217955323536	31.147004179395964
2	29.216329942611324	18.465346069674172	26.577014473968458	25.741309513746042
3	27.243801905083824	20.82294644827214	20.515682435953412	31.417569210690626
4	27.641170512548165	20.806725529525128	21.513737079493026	30.03836687843368
5	27.810998818126443	20.69452382936422	20.83501068615565	30.65946666635369
6	29.320205577926778	23.832180996834644	22.909803212771276	23.9378102124673
7	27.94280060607697	24.195428132815827	22.289290253827005	25.572481007280203
8	25.275897642942734	26.649546909347833	22.432241792316532	25.6423136553929
9	24.864647896746266	26.15168120633259	23.62843397645877	25.355236920462374
10	26.789916400344115	23.084560881745748	23.238662170774273	26.886860547135864
11	25.041752881623687	24.922391867958783	23.25990537969595	26.775949870721576
12	26.984626254493644	23.55813186516548	23.0710638153038	26.38617806503708
13	25.183648127956886	22.409824925443377	23.289951023253685	29.11657592334605
14	25.52400893388433	24.5096163664254	22.965199868080845	27.001174831609426
15	25.48445666091966	23.198640434629013	22.939262027353273	28.37764087709806
16	25.536801805555392	24.712541826235245	22.521674528218846	27.228981839990517
17	25.72611283312814	22.89841873064198	22.637279836439028	28.738188599790853
18	27.410664090878683	23.49862740702575	22.76603011371572	26.324678388379848
19	25.45675833326487	22.822130963796173	24.651394246963456	27.069716455975502
20	25.50628669881707	23.06953805996688	22.731172472556942	28.69300276865911
21	25.39162031695807	23.447103822956045	24.76289175235348	26.398384107732408
22	27.50467409279175	22.56122680118352	22.883161177272818	27.05093792875192
23	25.80474791587689	24.829672889792345	22.65019007390524	26.71538912042552
24	25.777284319812406	25.360518381244006	22.707581947732315	26.154615351211273
25	25.951455159811136	22.703239413311863	22.56333938549617	28.78196604138083
26	25.9236394663612	23.268238351151417	24.28415667394726	26.52396550854012
27	25.906856157655124	23.978184045998002	22.296214835740702	27.818744960606175
28	25.94899047811304	23.28337853872543	22.255019441643967	28.512611541517565
29	25.999105672640976	25.737732046847732	22.171102898113578	26.092059382397714
30	27.78952087761447	24.32171372839442	22.20173537064705	25.687030023344054
31	25.90814718140175	23.680779121094506	24.203409006885853	26.207664690617893
32	26.04616935649508	23.860935616645758	22.2241522375202	27.868742789338963
33	26.03419804539005	26.13395897126533	22.28870342485127	25.54313955849335
34	26.270338025226604	23.841452894651287	22.089768402076434	27.798440678045672
35	26.475493435144248	26.07457187892075	21.782035287199967	25.667899398735035
36	26.541922475197673	26.77712352867305	21.738962040380873	24.9419919557484
37	26.499436057354313	24.593650275750935	23.32574759077364	25.581166076121107
38	28.58925140574881	24.823217771059237	21.295906163699463	25.291624659492484
39	26.86810305255215	27.12535896525955	21.155059669079755	24.851478313108547
40	26.732524526517043	24.952672243106814	21.02866424814885	27.2861389822273
41	28.532681092487767	25.236814833158654	20.973971787610164	25.256532286743415
42	26.732759258107336	25.60252665083793	20.885125880683585	26.77958821037115
43	26.78792118182661	27.23461539815759	20.617883965132968	25.35957945488283
44	26.841674716004114	25.711090011349274	22.414871654634716	25.032363618011892
45	26.870077238429786	25.985843337789323	20.33996176222394	26.804117661556955
46	26.776536699697314	25.86718651889531	22.101035518410587	25.255241262996798
47	28.915997779439156	25.898875283585106	19.95124624869577	25.23388068827997
48	28.797693057930584	26.474789240373365	19.858996733709922	24.868520967986132
49	28.766708488011673	26.154497985416125	19.701609202417266	25.37718432415494
50	28.730442457311128	26.681822503013368	19.469107562230278	25.118627477445227
51	28.524582852622597	26.914324143200353	19.6640521479701	24.89704085620695
52	28.49782345132899	26.714802291449786	19.2925894063286	25.49478485089263
53	28.66319185669167	26.955284805706793	19.075580051101067	25.305943286500472
54	28.482800629550127	27.170651039802262	19.13273719333785	25.213811137309765
55	28.536788895317926	26.788508010802346	19.106799352610274	25.567903741269454
56	28.26684756647892	27.206799704707656	19.024291198621658	25.502061530191767
57	28.06791254370409	27.338132029477592	19.045534407543336	25.548421019274986
58	28.009933840901276	27.300222877644988	18.748716311615578	25.941126969838162
59	27.99526311650785	27.62450456963723	18.694258582667185	25.68597373118773
60	27.727551737776647	27.71968822950177	18.644378119729545	25.90838191299204
61	28.013689546345994	27.614763208640003	18.5043607261187	25.86718651889531
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	8.0
2	2.0
3	2.0
4	3.0
5	4.0
6	6.5
7	6.5
8	4.0
9	8.5
10	13.0
11	18.0
12	24.0
13	25.0
14	24.0
15	23.0
16	46.0
17	96.0
18	123.0
19	142.5
20	203.5
21	245.0
22	366.5
23	488.0
24	592.0
25	967.5
26	1239.0
27	1556.0
28	1873.0
29	2444.5
30	3611.5
31	4207.0
32	4856.0
33	5505.0
34	6752.0
35	9047.5
36	10096.0
37	11901.0
38	13706.0
39	15887.0
40	20624.5
41	23181.0
42	25747.0
43	30565.0
44	32817.0
45	35999.5
46	39182.0
47	41383.0
48	46019.0
49	48454.0
50	50803.0
51	53152.0
52	55243.0
53	58557.0
54	59780.0
55	60293.5
56	60807.0
57	60558.0
58	58182.5
59	56056.0
60	53599.0
61	53521.0
62	55900.0
63	44617.5
64	33335.0
65	29288.0
66	22092.0
67	18943.0
68	16168.5
69	13394.0
70	11279.0
71	7503.0
72	5842.0
73	4732.0
74	3622.0
75	2883.5
76	1698.5
77	1252.0
78	990.5
79	729.0
80	553.0
81	294.5
82	212.0
83	142.0
84	49.5
85	27.0
86	26.0
87	25.0
88	18.5
89	9.0
90	6.0
91	5.0
92	4.0
93	2.5
94	1.5
95	2.0
96	3.5
97	5.0
98	3.5
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03532710433936554
3	1.1736579514739384E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.0984569918911973
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	852037.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.26897833777561
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.89674953342727	63.22450394436975
2	12.666903301530894	19.32183547022316
3	2.6790672218913243	6.129891597356223
4	0.7424763307973281	2.2651164475957013
5	0.2776227242747603	1.0587000771892972
6	0.16465600553925588	0.75348871917949
7	0.10918895841048766	0.5829411212613637
8	0.07152157614983957	0.43639020336445467
9	0.067665871636346	0.4644726208235261
>10	0.32181305215882905	3.813259069236716
>50	0.0013345281049289709	0.06768575825877697
>100	8.340800655806068E-4	0.15801825518308457
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	1.6681601311612136E-4	1.7236967159584782
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGACGCATCTGGGGGGGGGGGGGGG	13548	1.5900717926568917	TruSeq Adapter, Index 25 (97% over 38bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9161139715763518	0.0	0.0	0.0	0.0
2	1.9188133848647417	0.0	0.0	0.0	0.0
3	1.9232732850803427	0.0	0.0	0.0	0.0
4	1.9266768931396172	0.0	0.0	0.0	0.0
5	1.9311367933552182	0.0	0.0	0.0	0.0
6	1.9416997149184836	0.0	0.0	0.0	0.0
7	1.9496805889885063	0.0	0.0	0.0	1.1736579514739384E-4
8	2.0388785933005256	0.0	0.0	0.0	1.1736579514739384E-4
9	2.0507325386104123	0.0	0.0	0.0	1.1736579514739384E-4
10	2.059887070631909	0.0	0.0	0.0	1.1736579514739384E-4
11	2.0712715527612064	1.1736579514739384E-4	0.0	0.0	1.1736579514739384E-4
12	2.096505198717896	1.1736579514739384E-4	0.0	0.0	1.1736579514739384E-4
13	2.1350011795262414	1.1736579514739384E-4	0.0	0.0	2.3473159029478767E-4
14	2.1788959869113667	1.1736579514739384E-4	0.0	0.0	2.3473159029478767E-4
15	2.2408651267491906	1.1736579514739384E-4	0.0	0.0	2.3473159029478767E-4
16	2.315861869848375	1.1736579514739384E-4	0.0	0.0	2.3473159029478767E-4
17	2.390037052381528	1.1736579514739384E-4	0.0	0.0	2.3473159029478767E-4
18	2.4748925222730938	1.1736579514739384E-4	0.0	0.0	2.3473159029478767E-4
19	2.604933823296406	1.1736579514739384E-4	0.0	0.0	2.3473159029478767E-4
20	2.7484721907616687	1.1736579514739384E-4	0.0	0.0	2.3473159029478767E-4
21	2.936844291973236	1.1736579514739384E-4	0.0	0.0	2.3473159029478767E-4
22	3.1403565807588167	1.1736579514739384E-4	0.0	0.0	2.3473159029478767E-4
23	3.3926930403257134	1.1736579514739384E-4	0.0	0.0	2.3473159029478767E-4
24	3.693501573288484	1.1736579514739384E-4	0.0	0.0	2.3473159029478767E-4
25	4.043251642827718	1.1736579514739384E-4	0.0	0.0	2.3473159029478767E-4
26	4.436896519752077	1.1736579514739384E-4	0.0	0.0	2.3473159029478767E-4
27	4.901547702740609	1.1736579514739384E-4	0.0	0.0	2.3473159029478767E-4
28	5.396948724057758	1.1736579514739384E-4	0.0	0.0	2.3473159029478767E-4
29	5.957839859067153	1.1736579514739384E-4	0.0	0.0	2.3473159029478767E-4
30	6.5794091101677505	1.1736579514739384E-4	0.0	0.0	2.3473159029478767E-4
31	7.257548674529392	1.1736579514739384E-4	0.0	0.0	3.520973854421815E-4
32	7.969841685278926	1.1736579514739384E-4	0.0	0.0	3.520973854421815E-4
33	8.773914747833722	1.1736579514739384E-4	0.0	0.0	3.520973854421815E-4
34	9.63232817354176	1.1736579514739384E-4	0.0	0.0	3.520973854421815E-4
35	10.532406456527122	1.1736579514739384E-4	0.0	0.0	3.520973854421815E-4
36	11.50572099568446	1.1736579514739384E-4	0.0	0.0	3.520973854421815E-4
37	12.528211803008555	1.1736579514739384E-4	0.0	0.0	3.520973854421815E-4
38	13.60785975256943	1.1736579514739384E-4	0.0	0.0	4.6946318058957535E-4
39	14.738092359838832	1.1736579514739384E-4	0.0	0.0	4.6946318058957535E-4
40	15.917853332660435	1.1736579514739384E-4	0.0	0.0	5.868289757369692E-4
41	17.178479338338594	1.1736579514739384E-4	0.0	0.0	7.04194770884363E-4
42	18.483117517197023	1.1736579514739384E-4	0.0	0.0	8.215605660317569E-4
43	19.804304273171237	1.1736579514739384E-4	0.0	0.0	8.215605660317569E-4
44	21.15694506224495	1.1736579514739384E-4	0.0	0.0	8.215605660317569E-4
45	22.52097033344796	1.1736579514739384E-4	0.0	0.0	8.215605660317569E-4
46	23.935462896564353	1.1736579514739384E-4	0.0	0.0	8.215605660317569E-4
47	25.307469041837386	1.1736579514739384E-4	0.0	0.0	8.215605660317569E-4
48	26.772076799481713	1.1736579514739384E-4	0.0	0.0	8.215605660317569E-4
49	28.238210312462957	1.1736579514739384E-4	0.0	0.0	8.215605660317569E-4
50	29.703287533287874	1.1736579514739384E-4	0.0	0.0	8.215605660317569E-4
>>END_MODULE