##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-25B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 427258 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.547392910138605 40.0 40.0 40.0 40.0 40.0 2 39.308380416516485 40.0 40.0 40.0 40.0 40.0 3 39.271952777946815 40.0 40.0 40.0 40.0 40.0 4 39.19392030108272 40.0 40.0 40.0 40.0 40.0 5 39.24520547303971 40.0 40.0 40.0 40.0 40.0 6 39.332562526623256 40.0 40.0 40.0 40.0 40.0 7 39.38718057941571 40.0 40.0 40.0 40.0 40.0 8 39.40811406691039 40.0 40.0 40.0 40.0 40.0 9 39.40107382424671 40.0 40.0 40.0 40.0 40.0 10-11 39.40252493809361 40.0 40.0 40.0 40.0 40.0 12-13 39.32999265081052 40.0 40.0 40.0 40.0 40.0 14-15 39.36173927697082 40.0 40.0 40.0 40.0 40.0 16-17 39.47091452939442 40.0 40.0 40.0 40.0 40.0 18-19 39.512238506944286 40.0 40.0 40.0 40.0 40.0 20-21 39.49395915348572 40.0 40.0 40.0 40.0 40.0 22-23 39.53326561468714 40.0 40.0 40.0 40.0 40.0 24-25 39.50983246656587 40.0 40.0 40.0 40.0 40.0 26-27 39.49107564984155 40.0 40.0 40.0 40.0 40.0 28-29 39.50583956298068 40.0 40.0 40.0 40.0 40.0 30-31 39.488821837691034 40.0 40.0 40.0 40.0 40.0 32-33 39.4968889727306 40.0 40.0 40.0 40.0 40.0 34-35 39.48874881570869 40.0 40.0 40.0 40.0 40.0 36-37 39.4711008022549 40.0 40.0 40.0 40.0 40.0 38-39 39.414499494618134 40.0 40.0 40.0 40.0 40.0 40-41 39.465386596772156 40.0 40.0 40.0 40.0 40.0 42-43 39.44132488961244 40.0 40.0 40.0 40.0 40.0 44-45 39.336488521945405 40.0 40.0 40.0 40.0 40.0 46-47 39.32718264402498 40.0 40.0 40.0 40.0 40.0 48-49 39.4220690592388 40.0 40.0 40.0 40.0 40.0 50-51 39.393047024165284 40.0 40.0 40.0 40.0 40.0 52-53 39.34774069973358 40.0 40.0 40.0 40.0 40.0 54-55 39.28064906065282 40.0 40.0 40.0 40.0 40.0 56-57 39.29354616090562 40.0 40.0 40.0 40.0 40.0 58-59 39.234203109125865 40.0 40.0 40.0 40.0 40.0 60-61 39.15730495538391 40.0 40.0 40.0 40.0 40.0 62-63 39.35276663055596 40.0 40.0 40.0 40.0 40.0 64-65 39.366514325453466 40.0 40.0 40.0 40.0 40.0 66-67 39.36831164434249 40.0 40.0 40.0 40.0 40.0 68-69 39.36696462205922 40.0 40.0 40.0 40.0 40.0 70-71 39.368169131997576 40.0 40.0 40.0 40.0 40.0 72-73 39.3646582400039 40.0 40.0 40.0 40.0 40.0 74-75 39.380640739989886 40.0 40.0 40.0 40.0 40.0 76-77 39.372736912877954 40.0 40.0 40.0 40.0 40.0 78-79 39.368007507901346 40.0 40.0 40.0 40.0 40.0 80-81 39.3848407766438 40.0 40.0 40.0 40.0 40.0 82-83 39.36354878259378 40.0 40.0 40.0 40.0 40.0 84-85 39.36183324977553 40.0 40.0 40.0 40.0 40.0 86-87 39.37345500479314 40.0 40.0 40.0 40.0 40.0 88-89 39.407774693706045 40.0 40.0 40.0 40.0 40.0 90-91 39.458381851430204 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0650784104407407 1101 2 0.04683577811133688 1101 3 -0.16444967041212522 1101 4 -0.06665824828731814 1101 5 0.06551751471306488 1101 6 -0.00394418796790319 1101 7 0.05927479375838374 1101 8 0.042464988189912845 1101 9 0.10970592746405572 1101 10-11 0.008052664099743367 1101 12-13 0.03150562232782761 1101 14-15 0.003685937688558738 1101 16-17 -0.043691202270736085 1101 18-19 -0.030066302526904565 1101 20-21 0.043151813018845075 1101 22-23 0.01609447063297864 1101 24-25 0.04735606846193008 1101 26-27 0.009583470542189332 1101 28-29 0.007599682614696235 1101 30-31 0.07881248714157607 1101 32-33 0.007307508922785644 1101 34-35 0.05844364864027085 1101 36-37 -0.11540067464004977 1101 38-39 -0.05259372033629717 1101 40-41 -0.09780088750462568 1101 42-43 0.04716373752848568 1101 44-45 -0.04808966812073834 1101 46-47 -0.011497138003939256 1101 48-49 0.02373074563798383 1101 50-51 -0.1689129113322636 1101 52-53 -0.02015474806410822 1101 54-55 0.07210175335813318 1101 56-57 -0.14372822046868095 1101 58-59 0.09413495121038551 1101 60-61 0.005198917512032608 1101 62-63 0.04782171306976579 1101 64-65 -0.06629738275288588 1101 66-67 0.05405279044370559 1101 68-69 0.03968953596532998 1101 70-71 0.2886149897949366 1101 72-73 -0.1175819458943792 1101 74-75 0.03326207350831112 1101 76-77 -0.31058976142463024 1101 78-79 -0.0655576636000319 1101 80-81 0.26072615021405454 1101 82-83 0.2081008764759531 1101 84-85 0.4080075948997859 1101 86-87 0.4440367811158126 1101 88-89 0.07774598454058435 1101 90-91 0.5079434600598347 1102 1 -0.020013608591490595 1102 2 -0.00479542756242779 1102 3 -0.025823222795644085 1102 4 -0.19339134820652504 1102 5 -0.05934827216187699 1102 6 -0.023306787842223287 1102 7 -0.11922596932627272 1102 8 -0.278928314620039 1102 9 -0.14657560588723584 1102 10-11 -0.14687686278776368 1102 12-13 -0.10327128762818916 1102 14-15 -0.049475028286835254 1102 16-17 -0.08439144466233017 1102 18-19 -0.14684518752556386 1102 20-21 -0.10217541563722676 1102 22-23 -0.1477263391345076 1102 24-25 -0.08585517132620879 1102 26-27 -0.21929165289797226 1102 28-29 -0.020352557263208837 1102 30-31 -0.11709030024047706 1102 32-33 -0.07590025706092263 1102 34-35 -0.09420324281727233 1102 36-37 -0.16233535883158368 1102 38-39 -0.18488551284868038 1102 40-41 -0.18056966746193837 1102 42-43 -0.21501973696553733 1102 44-45 0.03043930833641184 1102 46-47 -0.10352639078023174 1102 48-49 -0.10797375346785287 1102 50-51 -0.04970831078589555 1102 52-53 -0.1592607278730398 1102 54-55 -0.16617175239046134 1102 56-57 -0.13308432141158022 1102 58-59 -0.22422166863876924 1102 60-61 -0.2160445212963893 1102 62-63 -0.20076603294245388 1102 64-65 -0.26428985041722086 1102 66-67 -0.1721448205441476 1102 68-69 0.09820695866058315 1102 70-71 -0.1854227729942295 1102 72-73 -0.01568490202934214 1102 74-75 -0.1378211225895143 1102 76-77 -0.1956225259800135 1102 78-79 -0.1747494783164143 1102 80-81 -0.01631369798327853 1102 82-83 -0.7215396694188314 1102 84-85 -0.12848372576640088 1102 86-87 0.21944627728002075 1102 88-89 -0.13736872531097077 1102 90-91 0.5079434600598347 1103 1 0.0230858203093689 1103 2 0.24948889693718712 1103 3 0.15083773888486718 1103 4 -0.04935474021113606 1103 5 -0.06546209564360339 1103 6 0.20714774942334913 1103 7 0.05733122165864302 1103 8 0.006686112389168386 1103 9 -0.16637563110927545 1103 10-11 -0.19213097488173503 1103 12-13 -0.10443438417456719 1103 14-15 -0.0069708396259500205 1103 16-17 -0.07106555783724389 1103 18-19 -0.048767467598345604 1103 20-21 -0.03636029322023404 1103 22-23 -0.1293718973523852 1103 24-25 -0.028689048134530992 1103 26-27 -0.04119663785483141 1103 28-29 -0.05500620455093497 1103 30-31 -0.04102782163417373 1103 32-33 -0.15781029190489448 1103 34-35 0.04897742601299626 1103 36-37 0.015523461135316552 1103 38-39 -0.046403998696263216 1103 40-41 -0.07021143359410331 1103 42-43 0.022378020570506862 1103 44-45 -0.055156099669446235 1103 46-47 0.10415358493910531 1103 48-49 0.035116734504150315 1103 50-51 -0.09695193636895993 1103 52-53 -0.07031870175747912 1103 54-55 0.01102073352223698 1103 56-57 0.1388782763486205 1103 58-59 0.07127224392224463 1103 60-61 0.009782641680907034 1103 62-63 0.05338106405579879 1103 64-65 -0.04051447332529534 1103 66-67 0.02787408364953592 1103 68-69 -0.21304171134602257 1103 70-71 -0.028525607890912852 1103 72-73 0.10903241809420905 1103 74-75 -0.1947757403541388 1103 76-77 -0.05608312491425238 1103 78-79 -0.168137118624216 1103 80-81 -0.08458750983980678 1103 82-83 0.27628750975792116 1103 84-85 0.06257920615298929 1103 86-87 0.15089880797783906 1103 88-89 -0.3387673267095721 1103 90-91 -0.4787232066068299 1104 1 -6.229011131537732E-4 1104 2 0.00562776230552231 1104 3 0.025520211365815726 1104 4 -0.16112442691731843 1104 5 -0.02820553340820453 1104 6 0.11231286373325844 1104 7 -0.05105150484431675 1104 8 0.03716875421812915 1104 9 -0.11557122806101461 1104 10-11 0.15672592604968116 1104 12-13 0.04797882497022954 1104 14-15 0.06584880474323285 1104 16-17 0.03731716866572299 1104 18-19 0.01727825636439917 1104 20-21 0.05170067206341855 1104 22-23 0.053523953621365195 1104 24-25 0.01872839471051435 1104 26-27 -0.010713996025870642 1104 28-29 0.07539176327294683 1104 30-31 0.00977658141409421 1104 32-33 0.033044243097911874 1104 34-35 0.004771912749752971 1104 36-37 -0.05336751352091795 1104 38-39 0.03474412007728489 1104 40-41 -0.062115558491640854 1104 42-43 -0.1391216957062369 1104 44-45 -0.06395723177090673 1104 46-47 0.0050107985039957725 1104 48-49 -0.04687318864980483 1104 50-51 -0.02252662800780314 1104 52-53 -0.0321564465553692 1104 54-55 -0.15889699236921473 1104 56-57 -0.19843719458150133 1104 58-59 -0.17153846131900963 1104 60-61 -0.17854045156828846 1104 62-63 -0.10915168616107707 1104 64-65 -0.012833919440694785 1104 66-67 -0.08709138307114017 1104 68-69 -0.14504265666869998 1104 70-71 0.15917427507068282 1104 72-73 -0.31372383776366775 1104 74-75 0.007359171750437099 1104 76-77 -0.14233150220278645 1104 78-79 0.0397480149743501 1104 80-81 0.16920666400766038 1104 82-83 -0.07459404778899881 1104 84-85 -0.3668591581973004 1104 86-87 -0.5101384554123669 1104 88-89 -1.126257614199865 1104 90-91 -0.025389873273496733 1201 1 -0.06641067777565013 1201 2 0.018138206367403598 1201 3 0.0648235293531485 1201 4 0.13573927036470224 1201 5 -0.07401080562672036 1201 6 0.03208844958204082 1201 7 0.06688929064272742 1201 8 0.033330386239484255 1201 9 0.0499384141212218 1201 10-11 0.03445750384602064 1201 12-13 -0.05240613333764088 1201 14-15 -0.0071853284136551565 1201 16-17 0.01540847079490959 1201 18-19 0.00800897620559482 1201 20-21 0.022412438385600808 1201 22-23 0.0014634593719051736 1201 24-25 -0.01356828954516942 1201 26-27 0.05042371979470772 1201 28-29 -0.05105848934718438 1201 30-31 0.12220094958340155 1201 32-33 0.09626927137944108 1201 34-35 0.04927357409341937 1201 36-37 0.05148119862342071 1201 38-39 0.007821630088585607 1201 40-41 0.0030545753682460486 1201 42-43 0.12532654077021732 1201 44-45 0.020195401612042474 1201 46-47 0.13944854479962743 1201 48-49 -0.01645438839390323 1201 50-51 -0.10612164703487537 1201 52-53 0.051605005475970245 1201 54-55 0.11417327766303487 1201 56-57 0.030112876635705277 1201 58-59 0.08036154601278866 1201 60-61 0.16099460740603178 1201 62-63 0.009723352897069049 1201 64-65 0.07516338852653348 1201 66-67 0.05817710739209758 1201 68-69 -0.36597010973831345 1201 70-71 0.03233463884777166 1201 72-73 -0.05511360306724811 1201 74-75 -0.06336618344822398 1201 76-77 -0.10552754050571167 1201 78-79 -0.33615770282866464 1201 80-81 -0.32035034862627043 1201 82-83 0.5184415715624056 1201 84-85 0.02664794577698615 1201 86-87 0.1589580044707688 1201 88-89 0.36739317945093575 1201 90-91 -0.7647838126674387 1202 1 0.05396741358387658 1202 2 0.03113038953836167 1202 3 0.13605727567472314 1202 4 0.14535832771860413 1202 5 -0.049105086376236784 1202 6 0.1155154808547394 1202 7 -0.05900577125171225 1202 8 0.034285298253081464 1202 9 -0.010845097910674895 1202 10-11 0.05662625697302559 1202 12-13 -0.09581428156150196 1202 14-15 0.07983136506227595 1202 16-17 -0.09016982930031503 1202 18-19 -0.010578184397154189 1202 20-21 0.09505568863710323 1202 22-23 -0.0694851582920819 1202 24-25 0.01714221879080924 1202 26-27 -0.04023777869088718 1202 28-29 -0.10265063701154986 1202 30-31 0.0809151269334194 1202 32-33 -0.005139719697460521 1202 34-35 -0.11641119851715587 1202 36-37 -0.17128310428365978 1202 38-39 -0.09018617659129546 1202 40-41 -0.06089940689056306 1202 42-43 -0.1733016624575754 1202 44-45 -0.13688699386364078 1202 46-47 -0.0026581046473168612 1202 48-49 -0.11822934364585791 1202 50-51 -0.08804074338062406 1202 52-53 -0.1618191095237691 1202 54-55 -0.01679106689964982 1202 56-57 0.03131565222592059 1202 58-59 -0.009994414000111362 1202 60-61 0.04635880524444502 1202 62-63 -0.21959043774757703 1202 64-65 -0.2564937266352345 1202 66-67 -0.23877327342486865 1202 68-69 0.039005224959822726 1202 70-71 0.020400909481843144 1202 72-73 -0.09403351598623289 1202 74-75 0.12559979311454583 1202 76-77 0.10014771326297733 1202 78-79 -0.38012695672467345 1202 80-81 -0.09730365494167614 1202 82-83 -0.43197418384862374 1202 84-85 -0.05534997990992707 1202 86-87 -0.5357804313746684 1202 88-89 -0.16999283878615046 1202 90-91 -1.2126447752342813 1203 1 0.12678933953812077 1203 2 0.06644362124858816 1203 3 -0.014495173845567422 1203 4 -0.18859090320582794 1203 5 -0.1614453379385452 1203 6 -0.12622432365086667 1203 7 -0.11198358301004419 1203 8 0.08983819907764001 1203 9 -0.12884789068768043 1203 10-11 -0.07236844949336785 1203 12-13 -0.01521399378866306 1203 14-15 -0.09009663863309925 1203 16-17 -0.05781795001624346 1203 18-19 0.03393634494558739 1203 20-21 -0.010310584598428818 1203 22-23 -0.038228355543907355 1203 24-25 -0.04117292963520214 1203 26-27 -0.010661420663240051 1203 28-29 -0.03578990950907013 1203 30-31 0.06369123223066708 1203 32-33 -0.032778965246237135 1203 34-35 0.030321918404894177 1203 36-37 0.08625596462779583 1203 38-39 0.08473601162825162 1203 40-41 0.022521155331141074 1203 42-43 0.06207708510726917 1203 44-45 -0.07875728524861358 1203 46-47 0.05315967934738097 1203 48-49 0.03612330216072479 1203 50-51 -0.06849471589805489 1203 52-53 0.09956490856520617 1203 54-55 0.04031165260760616 1203 56-57 -0.04403447603574051 1203 58-59 0.037822108915065655 1203 60-61 -0.008825856712242341 1203 62-63 -0.06079902838939688 1203 64-65 -0.061609188300892015 1203 66-67 -0.25206201467874223 1203 68-69 -0.030190096897960927 1203 70-71 -0.29371448564679525 1203 72-73 -0.2798342698178615 1203 74-75 -0.32845213415490093 1203 76-77 -0.2189365866772519 1203 78-79 -0.1372679743939358 1203 80-81 -0.3722719060840447 1203 82-83 -0.3408147080478514 1203 84-85 -0.7201699288500834 1203 86-87 -0.10339211057537767 1203 88-89 -1.4040353919776365 1203 90-91 -1.7552144346770078 1204 1 -0.250895067558055 1204 2 -0.4196144383388827 1204 3 -0.48032999262156295 1204 4 -0.41253199479398006 1204 5 -0.2302849950134913 1204 6 -0.23468285857999405 1204 7 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36-37 -0.03002715658807631 2401 38-39 0.058193424777940095 2401 40-41 0.09098104811574359 2401 42-43 0.034499086441492466 2401 44-45 0.009843565738243854 2401 46-47 -0.16283540860648316 2401 48-49 0.15349987164648127 2401 50-51 0.017829132896942212 2401 52-53 0.012631855718048257 2401 54-55 -0.03545748314878239 2401 56-57 0.0494726563624468 2401 58-59 0.14909531421806577 2401 60-61 0.09585687848990432 2401 62-63 0.14607403025602395 2401 64-65 0.11589259428272669 2401 66-67 0.11132512441395193 2401 68-69 0.06700822572285858 2401 70-71 -0.17466004077940056 2401 72-73 -0.11701849713769974 2401 74-75 0.06159628732882538 2401 76-77 0.16186075010785572 2401 78-79 0.20199302299199218 2401 80-81 -0.05869085467786306 2401 82-83 0.20479881488167706 2401 84-85 -0.27809053594133104 2401 86-87 0.054962087693290584 2401 88-89 0.5959646080223635 2401 90-91 -0.025389873273496733 2402 1 0.08416986750687983 2402 2 -0.031952100676541306 2402 3 0.04044922815765517 2402 4 0.1777277454657593 2402 5 0.2229617582332608 2402 6 0.07375869986156403 2402 7 0.20973783282901337 2402 8 0.09189235407297502 2402 9 -0.03175935830503107 2402 10-11 0.05642791753829357 2402 12-13 0.06389643826294389 2402 14-15 0.07347606317489408 2402 16-17 -0.056217916063260986 2402 18-19 0.007037100399280405 2402 20-21 -0.00427544326916518 2402 22-23 0.10659805987074833 2402 24-25 0.1074689513596141 2402 26-27 0.15046242281353983 2402 28-29 0.02965020593104839 2402 30-31 -0.058013207120822585 2402 32-33 0.07292958158229368 2402 34-35 0.1071839382957478 2402 36-37 -5.457825097252567E-4 2402 38-39 -0.24694632538998462 2402 40-41 0.0953484900726167 2402 42-43 0.12368140241523662 2402 44-45 0.16669214512999986 2402 46-47 0.14874622241502067 2402 48-49 0.1797142319396201 2402 50-51 0.22085446678279652 2402 52-53 0.22101682819874924 2402 54-55 0.2873484511887128 2402 56-57 -0.006658164115172838 2402 58-59 0.2676899385584761 2402 60-61 0.13517432923562467 2402 62-63 -0.10801628509544514 2402 64-65 0.1035155818586162 2402 66-67 0.11796548686939445 2402 68-69 -0.03789571370694489 2402 70-71 0.17234098340161808 2402 72-73 0.25637158900816104 2402 74-75 0.05383541760475907 2402 76-77 0.014883698319316352 2402 78-79 0.33805551816772095 2402 80-81 0.254327891986037 2402 82-83 0.1377196448043989 2402 84-85 0.4917197161119162 2402 86-87 0.17297451005354247 2402 88-89 0.2369902490480058 2402 90-91 0.5079434600598347 2403 1 -0.06312692515732721 2403 2 -0.18873397694724048 2403 3 -0.024942249374397818 2403 4 0.13438514557363845 2403 5 0.1570557792282372 2403 6 0.004068645506976054 2403 7 0.04014321019924694 2403 8 0.13589052842258553 2403 9 -0.04686246605989197 2403 10-11 -0.03565124930126018 2403 12-13 0.021147174746907638 2403 14-15 0.12997164427110874 2403 16-17 -0.07227534106637279 2403 18-19 0.0940085953579981 2403 20-21 0.08843025358988399 2403 22-23 0.008809586363426547 2403 24-25 0.0664499065067119 2403 26-27 0.032122934825494553 2403 28-29 0.029368059961996096 2403 30-31 0.057309808521679884 2403 32-33 -0.01209099139306602 2403 34-35 0.10608087580824588 2403 36-37 0.037743231035065605 2403 38-39 0.16235374895146748 2403 40-41 -0.08121600953079877 2403 42-43 -0.008229248034588466 2403 44-45 0.14442092988044664 2403 46-47 0.04311067259457246 2403 48-49 0.04495869930079266 2403 50-51 -0.023414196048861413 2403 52-53 0.07128989916655826 2403 54-55 -0.036490823723987376 2403 56-57 0.051317355713273116 2403 58-59 -0.0136922507344579 2403 60-61 0.1093392664060886 2403 62-63 -0.009119158113705339 2403 64-65 -0.030197162964995528 2403 66-67 0.1745327469251805 2403 68-69 0.08671934429550276 2403 70-71 -0.030050637977694805 2403 72-73 0.09502499702532674 2403 74-75 0.03310043413519992 2403 76-77 -0.044649666311499914 2403 78-79 -0.02364343303058547 2403 80-81 -0.02387128607962552 2403 82-83 -0.0940324944348987 2403 84-85 0.557966340774378 2403 86-87 0.29968917469923895 2403 88-89 0.33181366462613227 2403 90-91 0.10794346005983613 2404 1 -0.08670269490270499 2404 2 -0.013317717014160735 2404 3 -0.0669962821503276 2404 4 -0.04172933758808739 2404 5 0.04278574258742651 2404 6 -0.1452668296804518 2404 7 0.008347810281556178 2404 8 -0.036012348567261654 2404 9 0.1050511864706678 2404 10-11 0.041021295535529134 2404 12-13 0.08907443388275738 2404 14-15 0.06018033587791649 2404 16-17 0.025830589258418968 2404 18-19 0.05834751991131526 2404 20-21 0.10341978620577663 2404 22-23 0.031934453408752006 2404 24-25 -0.06659581399325276 2404 26-27 0.048147873641497085 2404 28-29 0.055149038150474894 2404 30-31 -0.03386398981666616 2404 32-33 0.032381388784472165 2404 34-35 0.12856492503490813 2404 36-37 0.033574131297328336 2404 38-39 0.1459672117690971 2404 40-41 0.05816510089824334 2404 42-43 0.06913236950087764 2404 44-45 0.015658596022575466 2404 46-47 0.026632550478431938 2404 48-49 -0.005235391654601074 2404 50-51 0.15131720065148357 2404 52-53 0.048847123791382785 2404 54-55 0.041342060935221525 2404 56-57 0.07119366419038187 2404 58-59 -0.0025063422218352116 2404 60-61 -0.08638960026600984 2404 62-63 0.3065674672286036 2404 64-65 0.013314245727229945 2404 66-67 0.18312329713222653 2404 68-69 0.1302707114566175 2404 70-71 0.2169125300720367 2404 72-73 0.1709199035558413 2404 74-75 0.13519742140344704 2404 76-77 0.06496956064264481 2404 78-79 0.24002824542350254 2404 80-81 0.01119351521268186 2404 82-83 0.3559810523197626 2404 84-85 0.20282918771481206 2404 86-87 0.2733517447591325 2404 88-89 -0.520164424235702 2404 90-91 -0.4444374923211143 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 11.0 31 253.0 32 1692.0 33 3118.0 34 4279.0 35 5318.0 36 7376.0 37 11003.0 38 27373.0 39 180344.0 40 186491.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.113322629418292 13.39378080691292 26.16241240655529 31.330484157113503 2 27.999260251140058 18.447604243723838 27.118818649162396 26.434316855973705 3 27.6650173899611 19.420818334589406 21.212709884893904 31.70145439055559 4 27.620547772072147 21.22090165661029 20.427001951982174 30.731548619335392 5 27.922941173717053 21.270286337529082 21.307266335563053 29.499506153190815 6 27.678124224707318 24.19942985268854 23.579195708447823 24.543250214156316 7 26.210860884992204 24.41475642351928 23.014665611878538 26.35971707960998 8 25.534688642459592 24.95775386300549 23.179203198067675 26.32835429646724 9 25.00784069578568 24.687191345744257 24.200600105790883 26.10436785267918 10-11 25.09315214694634 23.39827457882591 23.90897303268751 27.59960024154024 12-13 25.27910536490832 23.311324773321974 23.782936773565385 27.626633088204315 14-15 25.685534267351347 23.044038964747298 23.7133067139761 27.55712005392526 16-17 25.694428190929138 23.032102383103418 23.32489970930913 27.948569716658316 18-19 25.73491894827013 23.247663004554624 23.407753628954868 27.609664418220376 20-21 25.695715469341707 23.279025787697364 23.439584513338545 27.58567422962238 22-23 25.756802681283908 22.631875822102803 23.557194950123815 28.054126546489478 24-25 25.770377617271063 23.036666370202546 23.529342926288095 27.663613086238293 26-27 25.981491276933376 23.28897293906726 23.27598312963128 27.453552654368085 28-29 26.08072874001189 22.97358972798637 23.24719490331369 27.698486628688052 30-31 25.999262731054824 23.159489997132845 23.36627638223299 27.474970889579346 32-33 26.064470514297682 23.08281040212537 23.438705162021385 27.414013921555558 34-35 26.117828188099878 22.82259788977205 23.45297808515448 27.60659583697359 36-37 26.22319547579801 23.158076332686502 23.424531782619702 27.19419640889578 38-39 26.392466782086316 23.143681817233684 23.415630671033146 27.04822072964686 40-41 26.328544602083102 22.842938550708155 23.501202936871877 27.327313910336866 42-43 26.211553715572787 23.03334167546833 23.5861848588836 27.168919750075293 44-45 26.416917068198114 23.12969317663235 23.480255320662128 26.973134434507408 46-47 26.34871468543248 22.811721145537973 23.4670896460511 27.372474522978447 48-49 26.43568734470026 23.09490732338315 23.40756922284956 27.061836109067027 50-51 26.527464746769112 23.26660661144858 23.44519493993894 26.76073370184337 52-53 26.521137039878734 23.04826243988248 23.297153657578082 27.133446862660705 54-55 26.642415584363473 23.229473692658665 23.332403686218015 26.795707036759854 56-57 26.65490174054666 23.19497665014969 23.285481620362972 26.86463998894068 58-59 26.699331973447173 22.9362792860364 23.300551197844744 27.06383754267169 60-61 26.979702197341865 23.200172639222107 23.15650507127715 26.66362009215888 62-63 26.98503037235283 23.10630878743795 23.045230929827092 26.863429910382123 64-65 26.958623197040104 22.912293887427477 22.959344228236926 27.169738687295492 66-67 26.888711391496823 23.315034555583384 23.122118957849633 26.674135095070163 68-69 26.995844352096714 23.13940309784662 22.89988666414809 26.964865885908573 70-71 27.156685458993724 22.971173279438357 22.817147112009362 27.05499414955856 72-73 27.197150793113128 22.91559805278388 22.857046077017625 27.030205077085366 74-75 27.2645799818533 23.39532527679282 22.541985115253805 26.798109626100075 76-77 27.347585526144897 22.871912097046522 22.468633919526194 27.311868457282383 78-79 27.264343789865208 23.118362202788067 22.28916588537264 27.32812812197409 80-81 27.73815303035961 23.168340864109936 22.132081108331157 26.961424997199295 82-83 28.178706202610027 22.47146378792658 21.902492026217097 27.447337983246296 84-85 28.209080503624573 22.74704311331553 21.38725677222434 27.65661961083556 86-87 28.619804585553133 21.989517503181418 21.083622716390224 28.307055194875225 88-89 28.431921596079807 21.249562478123906 20.47602380119006 29.842492124606228 90-91 24.0509511438867 24.428056649627084 22.65147071147239 28.869521495013828 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.5 8 1.0 9 1.0 10 4.0 11 7.5 12 9.0 13 15.0 14 31.0 15 54.5 16 75.5 17 91.5 18 108.0 19 146.0 20 214.0 21 304.0 22 425.5 23 557.0 24 708.5 25 887.0 26 1122.0 27 1423.5 28 1772.0 29 2157.5 30 2563.5 31 3079.5 32 3714.5 33 4367.5 34 5162.0 35 6029.5 36 7009.0 37 8134.5 38 9350.5 39 10693.0 40 12089.0 41 13573.5 42 15031.0 43 16446.0 44 17776.5 45 19094.0 46 20647.0 47 22160.0 48 23179.0 49 24085.5 50 25099.5 51 26055.0 52 26842.5 53 27461.5 54 27951.5 55 28380.5 56 28715.5 57 28629.5 58 28313.0 59 28002.5 60 27449.5 61 26516.5 62 25532.5 63 24047.0 64 22003.0 65 20028.0 66 17874.5 67 15886.5 68 14180.0 69 12236.5 70 10216.5 71 8431.0 72 6868.0 73 5392.5 74 4112.0 75 3175.0 76 2454.5 77 1814.0 78 1333.0 79 982.0 80 681.0 81 453.0 82 311.5 83 223.5 84 155.0 85 93.5 86 50.0 87 33.0 88 23.0 89 12.5 90 5.0 91 4.0 92 3.5 93 2.5 94 2.5 95 1.5 96 1.5 97 2.0 98 2.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.020128353360264757 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.065272025400068 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.776258547406003E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 4.2548483997515167E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 4632.0 32-33 10583.0 34-35 11786.0 36-37 12978.0 38-39 14154.0 40-41 15500.0 42-43 16043.0 44-45 16782.0 46-47 17317.0 48-49 17673.0 50-51 18101.0 52-53 18067.0 54-55 17774.0 56-57 17535.0 58-59 17158.0 60-61 16835.0 62-63 16647.0 64-65 16025.0 66-67 15565.0 68-69 14976.0 70-71 14233.0 72-73 13826.0 74-75 12968.0 76-77 12055.0 78-79 11691.0 80-81 10942.0 82-83 10178.0 84-85 9723.0 86-87 9076.0 88-89 8422.0 90-91 8013.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.84195152392982 #Duplication Level Percentage of deduplicated Percentage of total 1 99.84767659144553 99.68986886020127 2 0.1474203815749832 0.29437477181697413 3 0.003830797816065524 0.011474229895487707 4 0.0010722291634195453 0.004282138086267122 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0018724049637455589 0.0 0.0 0.0 0.0 2 0.0018724049637455589 0.0 0.0 0.0 0.0 3 0.0018724049637455589 0.0 0.0 0.0 0.0 4 0.002106455584213754 0.0 0.0 0.0 0.0 5 0.0023405062046819485 0.0 0.0 0.0 0.0 6 0.0028086074456183385 0.0 0.0 0.0 0.0 7 0.003276708686554728 0.0 0.0 0.0 2.3405062046819486E-4 8 0.01591544219183725 0.0 0.0 0.0 2.3405062046819486E-4 9 0.016149492812305447 0.0 0.0 0.0 2.3405062046819486E-4 10-11 0.016149492812305447 1.1702531023409743E-4 0.0 0.0 2.3405062046819486E-4 12-13 0.016149492812305447 2.3405062046819486E-4 0.0 0.0 3.510759307022923E-4 14-15 0.016149492812305447 2.3405062046819486E-4 0.0 0.0 4.681012409363897E-4 16-17 0.01626651812253954 2.3405062046819486E-4 0.0 0.0 4.681012409363897E-4 18-19 0.01638354343277364 2.3405062046819486E-4 0.0 0.0 4.681012409363897E-4 20-21 0.016617594053241834 2.3405062046819486E-4 0.0 0.0 4.681012409363897E-4 22-23 0.016617594053241834 2.3405062046819486E-4 0.0 0.0 4.681012409363897E-4 24-25 0.016617594053241834 2.3405062046819486E-4 0.0 0.0 4.681012409363897E-4 26-27 0.016617594053241834 2.3405062046819486E-4 0.0 0.0 4.681012409363897E-4 28-29 0.016734619363475933 2.3405062046819486E-4 0.0 0.0 4.681012409363897E-4 30-31 0.01685164467371003 2.3405062046819486E-4 0.0 0.0 4.681012409363897E-4 32-33 0.017202720604412323 2.3405062046819486E-4 0.0 0.0 4.681012409363897E-4 34-35 0.01731974591464642 2.3405062046819486E-4 0.0 0.0 4.681012409363897E-4 36-37 0.017436771224880517 2.3405062046819486E-4 0.0 0.0 4.681012409363897E-4 38-39 0.017904872465816908 2.3405062046819486E-4 0.0 0.0 4.681012409363897E-4 40-41 0.0182559483965192 2.3405062046819486E-4 0.0 0.0 8.19177171638682E-4 42-43 0.018489999016987394 2.3405062046819486E-4 0.0 0.0 9.362024818727794E-4 44-45 0.018724049637455588 2.3405062046819486E-4 0.0 0.0 9.362024818727794E-4 46-47 0.018724049637455588 2.3405062046819486E-4 0.0 0.0 9.362024818727794E-4 48-49 0.018841074947689686 2.3405062046819486E-4 0.0 0.0 9.362024818727794E-4 50-51 0.01907512556815788 3.510759307022923E-4 0.0 0.0 9.362024818727794E-4 52-53 0.019426201498860173 4.681012409363897E-4 0.0 0.0 9.362024818727794E-4 54-55 0.019426201498860173 4.681012409363897E-4 0.0 0.0 9.362024818727794E-4 56-57 0.019426201498860173 4.681012409363897E-4 0.0 0.0 9.362024818727794E-4 58-59 0.019426201498860173 4.681012409363897E-4 0.0 0.0 9.362024818727794E-4 60-61 0.019426201498860173 4.681012409363897E-4 0.0 0.0 9.362024818727794E-4 62-63 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