##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-25A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 512039 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.56359574173061 40.0 40.0 40.0 40.0 40.0 2 39.31313435109435 40.0 40.0 40.0 40.0 40.0 3 39.31251330465062 40.0 40.0 40.0 40.0 40.0 4 39.20180298766305 40.0 40.0 40.0 40.0 40.0 5 39.25299440081712 40.0 40.0 40.0 40.0 40.0 6 39.339511248166644 40.0 40.0 40.0 40.0 40.0 7 39.3693839727052 40.0 40.0 40.0 40.0 40.0 8 39.398803606756516 40.0 40.0 40.0 40.0 40.0 9 39.39749901862944 40.0 40.0 40.0 40.0 40.0 10-11 39.401815096115726 40.0 40.0 40.0 40.0 40.0 12-13 39.31744652262816 40.0 40.0 40.0 40.0 40.0 14-15 39.349330812692 40.0 40.0 40.0 40.0 40.0 16-17 39.459377117758606 40.0 40.0 40.0 40.0 40.0 18-19 39.491089545913496 40.0 40.0 40.0 40.0 40.0 20-21 39.47309872880777 40.0 40.0 40.0 40.0 40.0 22-23 39.50947486421933 40.0 40.0 40.0 40.0 40.0 24-25 39.48895689586145 40.0 40.0 40.0 40.0 40.0 26-27 39.46882952275119 40.0 40.0 40.0 40.0 40.0 28-29 39.49339796382698 40.0 40.0 40.0 40.0 40.0 30-31 39.47100915927591 40.0 40.0 40.0 40.0 40.0 32-33 39.47832697206597 40.0 40.0 40.0 40.0 40.0 34-35 39.47895285980367 40.0 40.0 40.0 40.0 40.0 36-37 39.45275415116703 40.0 40.0 40.0 40.0 40.0 38-39 39.405996141172054 40.0 40.0 40.0 40.0 40.0 40-41 39.45815498586721 40.0 40.0 40.0 40.0 40.0 42-43 39.430860251618185 40.0 40.0 40.0 40.0 40.0 44-45 39.343874653585644 40.0 40.0 40.0 40.0 40.0 46-47 39.330932547305274 40.0 40.0 40.0 40.0 40.0 48-49 39.41231675483173 40.0 40.0 40.0 40.0 40.0 50-51 39.372071294668395 40.0 40.0 40.0 40.0 40.0 52-53 39.327362333851966 40.0 40.0 40.0 40.0 40.0 54-55 39.255198410444166 40.0 40.0 40.0 40.0 40.0 56-57 39.27471934922421 40.0 40.0 40.0 40.0 40.0 58-59 39.21779008843417 40.0 40.0 40.0 40.0 40.0 60-61 39.146909681297025 40.0 40.0 40.0 40.0 40.0 62-63 39.38374198673305 40.0 40.0 40.0 40.0 40.0 64-65 39.38583682569108 40.0 40.0 40.0 40.0 40.0 66-67 39.38823610659507 40.0 40.0 40.0 40.0 40.0 68-69 39.39874130703788 40.0 40.0 40.0 40.0 40.0 70-71 39.38004085055691 40.0 40.0 40.0 40.0 40.0 72-73 39.384900078250084 40.0 40.0 40.0 40.0 40.0 74-75 39.38751796704918 40.0 40.0 40.0 40.0 40.0 76-77 39.36234321699164 40.0 40.0 40.0 40.0 40.0 78-79 39.36252282337527 40.0 40.0 40.0 40.0 40.0 80-81 39.36892845744305 40.0 40.0 40.0 40.0 40.0 82-83 39.33917317845115 40.0 40.0 40.0 40.0 40.0 84-85 39.33982603359205 40.0 40.0 40.0 40.0 40.0 86-87 39.30900375074049 40.0 40.0 40.0 40.0 40.0 88-89 39.36516972506349 40.0 40.0 40.0 40.0 40.0 90-91 39.39285162439478 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04288007959698348 1101 2 0.060180658991960456 1101 3 0.18028439335450486 1101 4 0.11725956737812737 1101 5 0.20355686604499823 1101 6 0.16115861395004316 1101 7 0.13233700996725872 1101 8 -0.010959037846035358 1101 9 -0.07135465232305194 1101 10-11 0.068021191202007 1101 12-13 0.015514807223894422 1101 14-15 0.05955287873523929 1101 16-17 0.050414894315125025 1101 18-19 0.09340037960516412 1101 20-21 0.04523886937280963 1101 22-23 0.0029047658319001357 1101 24-25 0.029097411737801337 1101 26-27 0.1253344219250323 1101 28-29 0.008419165382463234 1101 30-31 0.007295292770926665 1101 32-33 -0.010433021905328133 1101 34-35 0.05285181588315879 1101 36-37 -0.08617748690198113 1101 38-39 -0.017419502192808523 1101 40-41 -0.0802830146666551 1101 42-43 -0.05909346480392941 1101 44-45 -0.015625776997886476 1101 46-47 -0.05628448358812932 1101 48-49 0.13246318933403245 1101 50-51 0.09589386491454377 1101 52-53 0.09220643038500498 1101 54-55 0.1451853411189319 1101 56-57 0.1702109404326393 1101 58-59 0.17178933069543945 1101 60-61 0.06355049504203691 1101 62-63 0.12214970759147548 1101 64-65 0.14226694406891482 1101 66-67 0.06721021384873183 1101 68-69 0.04597329826542307 1101 70-71 0.26030755042058473 1101 72-73 -0.0631287280189099 1101 74-75 0.2059138174870796 1101 76-77 0.09997648903796374 1101 78-79 0.045817572500816084 1101 80-81 0.13571758633545272 1101 82-83 0.11329073890203745 1101 84-85 0.024074457389289705 1101 86-87 0.019182777483926827 1101 88-89 0.19287862457737504 1101 90-91 0.5963323206101023 1102 1 0.02216281825812416 1102 2 -0.11495694821077507 1102 3 0.098043562846577 1102 4 -0.03926668108775999 1102 5 -0.2620076259874935 1102 6 -0.13639001466978584 1102 7 -0.09707994047439428 1102 8 -0.08347241580961651 1102 9 -0.1136801337939275 1102 10-11 0.012498259137615264 1102 12-13 -0.0888933151199538 1102 14-15 0.02287836761430384 1102 16-17 -0.0031216540097602774 1102 18-19 -0.0551467500900884 1102 20-21 0.03299756967926015 1102 22-23 -0.005107936751336695 1102 24-25 -0.02302170214342425 1102 26-27 -0.13353553216886382 1102 28-29 -0.09779951956433308 1102 30-31 -0.06547391482395426 1102 32-33 -0.0988999663893324 1102 34-35 -0.03577703025278112 1102 36-37 -0.005388445838853784 1102 38-39 0.03848409234761618 1102 40-41 0.023803410200343933 1102 42-43 0.03076034687239826 1102 44-45 0.02238320628636359 1102 46-47 0.01866763651070613 1102 48-49 -0.11399159198288089 1102 50-51 -0.026447623023358346 1102 52-53 -0.1272647437166725 1102 54-55 -0.11258542968892726 1102 56-57 0.08967135736233445 1102 58-59 -0.15721268961880952 1102 60-61 -0.1082480315534653 1102 62-63 -0.18391215547438833 1102 64-65 -0.1241352818979351 1102 66-67 -0.029883361409083875 1102 68-69 -0.17967275060161114 1102 70-71 0.06400232563424879 1102 72-73 -0.20366805986539305 1102 74-75 -0.14513482060564087 1102 76-77 -0.1820351970384948 1102 78-79 -0.06351690434309631 1102 80-81 -0.5057045155517699 1102 82-83 0.02078140822312946 1102 84-85 0.030127088968242788 1102 86-87 0.06902928018757137 1102 88-89 0.09697192736514637 1102 90-91 -0.3114937663464161 1103 1 -0.03247898134686267 1103 2 0.05339967272888657 1103 3 -0.08094679840041152 1103 4 -0.027426232916063498 1103 5 0.16930594609640792 1103 6 0.1889002562232065 1103 7 0.05352197216750909 1103 8 -0.028749108754155372 1103 9 0.04793323660873483 1103 10-11 -0.016571074876935654 1103 12-13 0.12929815383316168 1103 14-15 0.005446707138560214 1103 16-17 -0.12793540451545482 1103 18-19 -0.08303720805262316 1103 20-21 0.007211395529644449 1103 22-23 0.06585026284847828 1103 24-25 -0.07351656170708765 1103 26-27 0.056979187380903795 1103 28-29 0.07121512493893789 1103 30-31 0.012376634307649681 1103 32-33 -0.0295745532174152 1103 34-35 -0.03517907714854118 1103 36-37 0.09487803912865189 1103 38-39 0.028410293684871135 1103 40-41 0.03316660395210391 1103 42-43 0.0948392297184455 1103 44-45 -0.027330619475250728 1103 46-47 0.0846590191722214 1103 48-49 -0.0464706817610363 1103 50-51 -0.10956402507936502 1103 52-53 -0.20094176164375455 1103 54-55 0.090033076033734 1103 56-57 -0.02280863596824645 1103 58-59 0.08923011453467922 1103 60-61 0.23684920522048003 1103 62-63 0.10884330977357592 1103 64-65 -0.0831112892089152 1103 66-67 0.15807585849832861 1103 68-69 0.2724116281857434 1103 70-71 0.03632744416236022 1103 72-73 0.04030325170462845 1103 74-75 0.13291769689603683 1103 76-77 -0.16302122917011275 1103 78-79 0.13881389556409118 1103 80-81 -0.16131175069388348 1103 82-83 -0.10562806175932593 1103 84-85 0.28478021767246275 1103 86-87 -0.14110160469578403 1103 88-89 -0.3790663240009735 1103 90-91 -0.09116767938989767 1104 1 0.04330580722280786 1104 2 -0.07004768801458283 1104 3 0.015820610302220928 1104 4 -0.03724681251635786 1104 5 0.11679511699794887 1104 6 -0.02272489849551107 1104 7 -0.012891975149130985 1104 8 -0.050241561582517136 1104 9 -0.021692850541640496 1104 10-11 -0.15577187820267113 1104 12-13 0.024954980211866484 1104 14-15 -0.14716870551090722 1104 16-17 0.05440247886467375 1104 18-19 -0.11337225342870738 1104 20-21 -0.04347140226935409 1104 22-23 -0.01392110723480755 1104 24-25 -0.04708086411347523 1104 26-27 -0.1216367234701039 1104 28-29 -0.07870088836323674 1104 30-31 -0.043610943609039055 1104 32-33 -5.857129538213712E-4 1104 34-35 -0.02984068257370609 1104 36-37 -0.04928976010486252 1104 38-39 -0.12891845569584603 1104 40-41 -0.08774142641745897 1104 42-43 -0.10146411067673711 1104 44-45 -0.08803514616339925 1104 46-47 -0.04775898757164043 1104 48-49 -0.06687454569429008 1104 50-51 -0.09367996653683974 1104 52-53 0.019677880928355762 1104 54-55 -0.08382648246104907 1104 56-57 0.04699283866261794 1104 58-59 -0.2092198145945403 1104 60-61 -0.016211887507971312 1104 62-63 -0.09319527483346235 1104 64-65 -0.016365711139464167 1104 66-67 -0.2281894963887936 1104 68-69 -0.12966277585746155 1104 70-71 -0.45065569189677745 1104 72-73 -0.10165195029706808 1104 74-75 -0.16364685867655027 1104 76-77 -0.2018608869857914 1104 78-79 -0.12220882363825325 1104 80-81 0.13029204347175494 1104 82-83 -0.12021819608668238 1104 84-85 -0.2478077558731684 1104 86-87 0.04955431071260108 1104 88-89 0.4023439324092877 1104 90-91 0.38011610439388477 1201 1 0.12241730770158199 1201 2 0.10207369373367925 1201 3 -0.0724810746607858 1201 4 0.0984780647225989 1201 5 0.06747215984425736 1201 6 0.08310983346223821 1201 7 0.045939391483443615 1201 8 0.08347619776846216 1201 9 0.012269041056740093 1201 10-11 0.13154407608411844 1201 12-13 -0.04379983030528223 1201 14-15 0.07620319503035944 1201 16-17 0.04858816314906278 1201 18-19 0.060668063377704584 1201 20-21 0.0960625898152756 1201 22-23 0.0897962782976478 1201 24-25 0.0952318456202832 1201 26-27 0.08282571112363257 1201 28-29 0.08710391894289415 1201 30-31 0.04592050842668982 1201 32-33 -0.00328930244552339 1201 34-35 0.06168453422999676 1201 36-37 -0.03561658377640953 1201 38-39 0.13232530383380947 1201 40-41 0.05176715101400475 1201 42-43 0.19896070489652118 1201 44-45 0.006653918420923333 1201 46-47 0.2745559041985146 1201 48-49 0.08932231859166961 1201 50-51 0.07814521083832204 1201 52-53 -0.02040714426934187 1201 54-55 0.050439170564942515 1201 56-57 0.00807828754068396 1201 58-59 0.06844275678946588 1201 60-61 0.1323814255005047 1201 62-63 0.1187666133230394 1201 64-65 0.04681352629816615 1201 66-67 0.12622152734559222 1201 68-69 0.131075620703065 1201 70-71 -0.04534541028281325 1201 72-73 0.011555747159249563 1201 74-75 0.23685934177050427 1201 76-77 -0.16115538018292597 1201 78-79 0.1818493599447777 1201 80-81 -0.1104493804605653 1201 82-83 -0.03229005532976004 1201 84-85 0.09677028145650013 1201 86-87 0.03480520439723733 1201 88-89 0.16313797707429956 1201 90-91 -1.4466784320780732 1202 1 0.019940373491849073 1202 2 0.15210233752551972 1202 3 0.14398307537238253 1202 4 -0.044431843014237415 1202 5 -0.07368143118269899 1202 6 -3.528994570203281E-4 1202 7 0.071228844537778 1202 8 0.06925229377038988 1202 9 0.09456717770270018 1202 10-11 0.08366560113989863 1202 12-13 -0.06219027533935417 1202 14-15 0.05221213490855092 1202 16-17 -0.004074955707601191 1202 18-19 -0.021545272568744167 1202 20-21 -0.02040080643389075 1202 22-23 0.05278925303080939 1202 24-25 -0.010446786111010908 1202 26-27 0.07525583534723523 1202 28-29 0.008627467154923352 1202 30-31 -0.01339475419165126 1202 32-33 0.08394611074261604 1202 34-35 0.0677666434120141 1202 36-37 -0.24237202776894406 1202 38-39 -0.10377842315786268 1202 40-41 -0.06407058888010653 1202 42-43 -0.2148807485285289 1202 44-45 -0.14701877970755817 1202 46-47 -0.16271283172337547 1202 48-49 -0.17008264862268874 1202 50-51 -0.11777264977486368 1202 52-53 -0.12896904390423458 1202 54-55 -0.1573461418262525 1202 56-57 -0.21765448151056432 1202 58-59 -0.21618140923008156 1202 60-61 -0.25944843642756155 1202 62-63 -0.3452080448200192 1202 64-65 -0.37748541859607343 1202 66-67 -0.3970056510799722 1202 68-69 -0.3697431752102105 1202 70-71 -0.17941213285354252 1202 72-73 -0.4152691464023732 1202 74-75 -0.30243273072994725 1202 76-77 0.06321514035973053 1202 78-79 -0.5064216508194761 1202 80-81 0.08670092288436848 1202 82-83 -0.2575758420183334 1202 84-85 -0.23076002111593397 1202 86-87 -0.26616980257248457 1202 88-89 -0.8904194018174607 1202 90-91 0.09633232061010233 1203 1 0.10898951235731857 1203 2 0.11396853046782951 1203 3 -0.04373521921993273 1203 4 0.03853515686532205 1203 5 0.03516476898591492 1203 6 -0.03521228762996742 1203 7 0.07997568302988611 1203 8 -0.021091557849509 1203 9 0.06846215542159939 1203 10-11 0.0947722794489465 1203 12-13 0.023648427539129102 1203 14-15 0.10190426425938881 1203 16-17 0.10327329191373735 1203 18-19 0.11389899456361263 1203 20-21 0.020465446727612857 1203 22-23 0.01553615174211842 1203 24-25 -0.02746247321206141 1203 26-27 0.012782181246308255 1203 28-29 -0.06882947611858725 1203 30-31 -0.06004957774940323 1203 32-33 0.006557405422825013 1203 34-35 -0.017949322321399563 1203 36-37 0.030182753483607883 1203 38-39 0.13532517385201004 1203 40-41 -0.0763498070071833 1203 42-43 -0.047986523016334104 1203 44-45 0.02428276983090427 1203 46-47 -0.0037472705124699246 1203 48-49 -0.01497609874207484 1203 50-51 -0.00764798163166347 1203 52-53 0.0695638027989034 1203 54-55 0.07920812204213235 1203 56-57 0.07648406704000621 1203 58-59 -0.13896615359876563 1203 60-61 0.09275999622881415 1203 62-63 -0.02130120249952938 1203 64-65 -0.04510792649436013 1203 66-67 -0.1393003889459905 1203 68-69 -0.1183482450050306 1203 70-71 0.11212953636164968 1203 72-73 -0.06844732396628217 1203 74-75 0.04144197949098327 1203 76-77 -0.10284066068518882 1203 78-79 -0.12657814942431145 1203 80-81 -0.09731469488231426 1203 82-83 -0.15935474628428636 1203 84-85 -0.24490140106024683 1203 86-87 -0.2625521395381796 1203 88-89 0.28582438638154883 1203 90-91 0.28864001291779573 1204 1 -0.07629977620237582 1204 2 -0.1747947020582572 1204 3 -0.026160990103626602 1204 4 -0.2019949001840402 1204 5 -0.29415333309038516 1204 6 -0.109632343884563 1204 7 -0.10763977748431586 1204 8 0.028523751463517044 1204 9 0.009869760840800268 1204 10-11 -0.15527182089435598 1204 12-13 -0.07953765264789325 1204 14-15 -0.14719095177414232 1204 16-17 -0.05657252165521243 1204 18-19 -0.1224148758182082 1204 20-21 -1.2020583146465924E-4 1204 22-23 -0.028464360015711065 1204 24-25 -0.01770805881615445 1204 26-27 -0.17719628227834505 1204 28-29 0.046929563482542846 1204 30-31 -0.04950620336560263 1204 32-33 -0.11798327248266105 1204 34-35 -0.1486766894088305 1204 36-37 -0.01840487671406521 1204 38-39 -0.06032382965766203 1204 40-41 -0.0321347492919557 1204 42-43 -0.08774308208315773 1204 44-45 -0.007871351690290851 1204 46-47 0.01011973002770361 1204 48-49 0.09531617865697228 1204 50-51 0.01433589303263716 1204 52-53 -0.032437567441306214 1204 54-55 -0.12493419403556061 1204 56-57 0.0010454411113727247 1204 58-59 0.016269227150424115 1204 60-61 -0.17294451946904843 1204 62-63 -0.06768185453753972 1204 64-65 -0.1274132877978289 1204 66-67 -0.055429660037894735 1204 68-69 0.10185347946094225 1204 70-71 -0.05501185893958649 1204 72-73 0.045097824940704356 1204 74-75 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2401 34-35 0.06484638748830207 2401 36-37 0.040978504170816166 2401 38-39 0.04893710438241072 2401 40-41 0.003952007391767154 2401 42-43 0.1026796648393642 2401 44-45 0.05354914542083833 2401 46-47 0.08928938861271263 2401 48-49 0.05846200935205559 2401 50-51 0.08785959574942126 2401 52-53 -0.07071148275669259 2401 54-55 -0.053706593642971256 2401 56-57 0.1397565360926052 2401 58-59 0.01590764626123331 2401 60-61 0.07508299497583693 2401 62-63 0.00754454755523426 2401 64-65 0.14863981169661855 2401 66-67 0.20756036435231096 2401 68-69 -0.020413056025596177 2401 70-71 0.2295914704985833 2401 72-73 0.13436184890360892 2401 74-75 -0.12326513814056028 2401 76-77 0.10971878636998866 2401 78-79 0.26720898664223824 2401 80-81 0.08009253106627057 2401 82-83 0.20029867516911537 2401 84-85 0.3920558358969828 2401 86-87 0.4140322420222162 2401 88-89 0.541335974849602 2401 90-91 0.5963323206101023 2402 1 -0.08235987568725989 2402 2 -0.04150924580975612 2402 3 0.022144389733945502 2402 4 0.10839703847431537 2402 5 0.015237084920798338 2402 6 0.06535790140928555 2402 7 -0.08901719071990044 2402 8 -0.05718572583791115 2402 9 0.02451175281739637 2402 10-11 0.00919496119206542 2402 12-13 0.004672542247568856 2402 14-15 0.049622098579632734 2402 16-17 0.047051881115017125 2402 18-19 0.026340925936175097 2402 20-21 -0.027120782680370326 2402 22-23 0.02727895983782247 2402 24-25 -0.008545194392134192 2402 26-27 0.06254871977004939 2402 28-29 0.0693415865280187 2402 30-31 0.13166183508199936 2402 32-33 0.01674198843182495 2402 34-35 0.1089990697281138 2402 36-37 -0.02858139360156997 2402 38-39 -0.288607343803001 2402 40-41 -0.02272654070815605 2402 42-43 0.0023408127032666926 2402 44-45 0.008988933336375737 2402 46-47 0.049913804587177424 2402 48-49 0.10096744824241455 2402 50-51 0.11497687239603493 2402 52-53 0.003771220930602226 2402 54-55 0.08161544015203503 2402 56-57 0.06625572079832409 2402 58-59 0.1327976591633231 2402 60-61 0.19446803100797894 2402 62-63 0.09162935920633686 2402 64-65 0.22435235112169494 2402 66-67 0.06755839940264963 2402 68-69 0.12082118445229639 2402 70-71 0.2139698799779879 2402 72-73 0.06745583079019468 2402 74-75 0.10593515309621182 2402 76-77 0.0615444460949206 2402 78-79 0.027336253983399672 2402 80-81 0.1509160063283943 2402 82-83 -0.05281376038845309 2402 84-85 0.20673137807020225 2402 86-87 -0.05337901129824019 2402 88-89 0.3754695409779316 2402 90-91 0.5963323206101023 2403 1 0.03530768983367949 2403 2 0.06299064337259352 2403 3 0.11952655363324993 2403 4 0.09340548832009432 2403 5 0.09498423598372341 2403 6 0.12221081868391082 2403 7 0.027469474222932888 2403 8 0.029522158837856693 2403 9 0.06430106076117426 2403 10-11 0.09416035376359133 2403 12-13 -0.03689042526438868 2403 14-15 0.008452764593705808 2403 16-17 0.021552855386858027 2403 18-19 0.017107276156892226 2403 20-21 0.011692521901942143 2403 22-23 -0.11437716376079266 2403 24-25 0.03256295901642403 2403 26-27 -0.030628722373904793 2403 28-29 -0.06469524648825598 2403 30-31 -0.07439550381683802 2403 32-33 0.006347758331514797 2403 34-35 0.03704743258748522 2403 36-37 0.0668150026283243 2403 38-39 0.10895384806192254 2403 40-41 0.029143597255377074 2403 42-43 -0.05178523800520907 2403 44-45 0.048861254931857445 2403 46-47 -0.026795260676429677 2403 48-49 0.018825835150138914 2403 50-51 0.05530859515782538 2403 52-53 0.10885467661739767 2403 54-55 -0.13139857300349433 2403 56-57 -0.022692113300294636 2403 58-59 0.011549352095613585 2403 60-61 -0.15553749532175942 2403 62-63 8.664077501521206E-4 2403 64-65 -0.07883929851828242 2403 66-67 -0.05443382526794238 2403 68-69 0.049288850018740504 2403 70-71 -0.002877451307966794 2403 72-73 0.11125530062381728 2403 74-75 0.006793808240395549 2403 76-77 -0.08671390707166182 2403 78-79 -0.006909559201439208 2403 80-81 0.15385646993030377 2403 82-83 0.03619315409120105 2403 84-85 0.05123329466404414 2403 86-87 -0.3598318341066076 2403 88-89 -0.6979174566870512 2403 90-91 -0.05584159243337439 2404 1 0.06526883472739797 2404 2 -0.02056276173092897 2404 3 0.03844637249313365 2404 4 0.054647979088564114 2404 5 0.06385881574232144 2404 6 0.047186247192350095 2404 7 0.07459047618865355 2404 8 -0.01540233549462755 2404 9 0.06584998328978742 2404 10-11 0.06978692679832932 2404 12-13 0.056079946568878825 2404 14-15 0.029285141093254197 2404 16-17 0.10184250045510623 2404 18-19 0.0373534962807085 2404 20-21 0.06314051760308104 2404 22-23 0.036052033601102096 2404 24-25 0.017658741905485442 2404 26-27 0.0649400272816365 2404 28-29 0.05993216243647481 2404 30-31 0.06775057484867375 2404 32-33 -0.010551689984680479 2404 34-35 0.02700939679053249 2404 36-37 0.10372658993207295 2404 38-39 0.13415217703597904 2404 40-41 0.08853660939728769 2404 42-43 0.04451312596089707 2404 44-45 0.03781828271888088 2404 46-47 0.12657924333441883 2404 48-49 0.06255904501898613 2404 50-51 0.06986630870716937 2404 52-53 0.14352529806696168 2404 54-55 0.10890650316704864 2404 56-57 0.03830458519095714 2404 58-59 -0.005236092262371983 2404 60-61 0.15952877199493543 2404 62-63 0.08055562759129486 2404 64-65 0.012758789033071594 2404 66-67 0.11157030250067379 2404 68-69 -0.047273399341833056 2404 70-71 0.0031959157362422275 2404 72-73 -0.02759401757751334 2404 74-75 0.01840819063384913 2404 76-77 0.13115651587856547 2404 78-79 0.14885926507967184 2404 80-81 0.023441365705664907 2404 82-83 0.2011111475831484 2404 84-85 0.12723852591838636 2404 86-87 0.0931833581709256 2404 88-89 0.21949689438983455 2404 90-91 0.5963323206101023 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 10.0 31 295.0 32 2134.0 33 3792.0 34 5236.0 35 6534.0 36 9110.0 37 13269.0 38 33229.0 39 221600.0 40 216830.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.361630657039793 13.38159788609852 25.039498944416344 32.21727251244534 2 27.943451660037976 18.33503934239211 26.687776900897802 27.033732096672107 3 27.620855524112514 19.160529414866502 20.585817040565427 32.63279802045555 4 27.856471870306756 20.793338007456462 19.841262091364133 31.508928030872646 5 28.000992111928973 21.07651956198649 20.936686463335803 29.985801862748733 6 27.81487347643441 24.378025892558966 23.253697472262854 24.553403158743766 7 26.26421034335275 24.50223518130455 22.789279722833612 26.444274752509084 8 25.445718001949068 25.024851622630308 23.133394135993548 26.396036239427072 9 24.86451227347917 24.721945008095087 24.22647493647945 26.1870677819463 10-11 24.940483049142742 23.36970426080826 23.922396536201344 27.767416153847655 12-13 25.189975763564888 23.292268752966084 23.90394091075094 27.61381457271809 14-15 25.468567823935285 22.9616298758493 23.830606653008854 27.73919564720656 16-17 25.88396586978726 22.63186983803968 23.42204402399036 28.0621202681827 18-19 25.749796402227176 23.18104675620412 23.39343292210164 27.67572391946707 20-21 25.63955089358428 23.11659854034556 23.449678637760012 27.794171928310146 22-23 25.697749585480796 22.586365491691062 23.53600018748572 28.179884735342426 24-25 25.756827116684473 22.86349281988286 23.566759563236396 27.812920500196274 26-27 26.02624018873562 23.03252291329371 23.475164977667717 27.466071920302948 28-29 26.07018215409373 22.735670525096722 23.380152683682297 27.813994637127248 30-31 25.95903994937851 22.997233590249113 23.507159171580877 27.536567288791503 32-33 25.95355064362255 22.92076594994608 23.59305665997578 27.532626746455595 34-35 26.12666850144489 22.610774735103895 23.546005714794518 27.7165510486567 36-37 26.07638258705178 23.03041302600534 23.53765977857865 27.35554460836423 38-39 26.360934273227425 22.989588947528507 23.480418576604748 27.169058202639317 40-41 26.209087033113764 22.757243945888955 23.51708569785466 27.51658332314262 42-43 26.22268494127569 22.964477423970397 23.57052002180519 27.242317612948717 44-45 26.352308279554933 23.0795848985256 23.498396458295428 27.06971036362404 46-47 26.32702750065561 22.77619037557839 23.511090617810858 27.38569150595514 48-49 26.461282851151292 23.06114304791912 23.474553725130153 27.003020375799437 50-51 26.490236417633128 23.164337946796067 23.484270661977618 26.86115497359318 52-53 26.639784554916663 22.866584176432056 23.4647354247034 27.028895843947886 54-55 26.53527744803218 23.1052697559745 23.407689890902788 26.951762905090533 56-57 26.67664195964961 23.0601027917608 23.39569542986246 26.867559818727127 58-59 26.590340247317425 22.910847485228572 23.314428737817334 27.18438352963667 60-61 26.887949381101894 23.101839038775374 23.180286878596718 26.82992470152601 62-63 26.936137152328172 22.995804630444916 23.223286890765007 26.844771326461903 64-65 26.851682216567713 22.905060785976815 23.17692960135708 27.066327396098387 66-67 26.938989641814647 23.093333068121353 23.11048896801046 26.85718832205354 68-69 27.06513663006687 23.07163103002284 22.915049946338645 26.948182393571646 70-71 27.164249738025216 22.86786329986817 22.918875398339956 27.049011563766662 72-73 27.30473573936089 23.028972234941513 22.883625414533295 26.782666611164302 74-75 27.232376185875605 23.055480764662097 22.74267885142305 26.969464198039244 76-77 27.384581532128305 22.84027303441352 22.552844261280548 27.222301172177627 78-79 27.49947316946652 23.080580756366544 22.5029583560726 26.916987718094333 80-81 27.60089072938235 22.80670911955828 22.50003255589848 27.092367595160894 82-83 27.9635578066719 22.68817813306579 21.905771362455802 27.442492697806507 84-85 28.472405389152062 22.302522604264478 21.235875631706108 27.989196374877352 86-87 28.95320962443399 21.83550859747255 20.884015508005564 28.327266270087897 88-89 29.022838421727364 21.34751436304069 20.0963866862922 29.53326052893975 90-91 23.777526821005083 24.17278373800113 21.908526256352342 30.141163184641446 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.5 8 3.0 9 3.0 10 5.5 11 9.5 12 13.5 13 18.5 14 23.5 15 26.5 16 41.0 17 79.5 18 129.0 19 179.0 20 237.5 21 317.5 22 408.0 23 543.0 24 747.5 25 998.5 26 1277.5 27 1613.5 28 2021.5 29 2440.0 30 2950.5 31 3508.5 32 4140.0 33 4837.0 34 5688.0 35 6804.5 36 7988.0 37 9186.5 38 10562.0 39 12210.0 40 13855.0 41 15479.0 42 17217.5 43 18850.0 44 20455.0 45 21998.0 46 23554.5 47 25129.0 48 26407.5 49 27700.5 50 29051.0 51 30168.0 52 30880.5 53 31573.5 54 32268.0 55 32821.5 56 33222.5 57 33308.5 58 33263.5 59 32846.5 60 32209.5 61 31230.5 62 29817.0 63 28182.5 64 26136.5 65 23850.5 66 21165.5 67 18750.0 68 16539.5 69 14176.5 70 12026.5 71 10034.5 72 8175.0 73 6401.0 74 4947.0 75 3870.5 76 2997.5 77 2200.0 78 1590.0 79 1114.0 80 766.5 81 544.0 82 383.5 83 259.5 84 167.5 85 108.5 86 63.5 87 39.5 88 31.0 89 21.5 90 12.5 91 9.0 92 7.0 93 6.0 94 5.5 95 4.0 96 1.5 97 1.0 98 1.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.02402160772909876 3 3.905952476276221E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.06367443060980267 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 4.143557700076863E-4 64-65 0.0 66-67 2.4863561207871804E-4 68-69 0.0 70-71 3.0730085368177153E-4 72-73 0.0 74-75 3.959502211381985E-4 76-77 4.5841699443481765E-4 78-79 0.0 80-81 6.511137300352252E-4 82-83 0.0 84-85 0.0010550303848750845 86-87 0.0 88-89 0.002373999952520001 90-91 0.005646208570944611 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 4594.0 32-33 10457.0 34-35 11643.0 36-37 13000.0 38-39 14607.0 40-41 15992.0 42-43 16788.0 44-45 18018.0 46-47 18791.0 48-49 19261.0 50-51 20280.0 52-53 20395.0 54-55 20406.0 56-57 20165.0 58-59 20568.0 60-61 20615.0 62-63 20395.0 64-65 19960.0 66-67 19571.0 68-69 19144.0 70-71 18662.0 72-73 18035.0 74-75 17482.0 76-77 16590.0 78-79 15964.0 80-81 15183.0 82-83 14573.0 84-85 13832.0 86-87 12893.0 88-89 12291.0 90-91 11884.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.82894343392046 #Duplication Level Percentage of deduplicated Percentage of total 1 99.83708380802769 99.66630592079171 2 0.15481957013867506 0.30910948219675344 3 0.007759764696964147 0.023239473329813 4 3.368571366815623E-4 0.001345123681723844 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0013670833666966774 0.0 0.0 0.0 0.0 2 0.0015623809905104884 0.0 0.0 0.0 0.0 3 0.0015623809905104884 0.0 0.0 1.9529762381381106E-4 0.0 4 0.0015623809905104884 0.0 0.0 1.9529762381381106E-4 0.0 5 0.0015623809905104884 0.0 0.0 1.9529762381381106E-4 0.0 6 0.0017576786143242995 0.0 0.0 1.9529762381381106E-4 0.0 7 0.0023435714857657328 0.0 0.0 1.9529762381381106E-4 0.0 8 0.010741369309759607 0.0 0.0 1.9529762381381106E-4 0.0 9 0.011327262181201041 1.9529762381381106E-4 0.0 1.9529762381381106E-4 0.0 10-11 0.011327262181201041 1.9529762381381106E-4 0.0 1.9529762381381106E-4 0.0 12-13 0.011327262181201041 1.9529762381381106E-4 0.0 1.9529762381381106E-4 0.0 14-15 0.011327262181201041 1.9529762381381106E-4 0.0 1.9529762381381106E-4 0.0 16-17 0.011327262181201041 1.9529762381381106E-4 0.0 1.9529762381381106E-4 0.0 18-19 0.011327262181201041 3.905952476276221E-4 0.0 1.9529762381381106E-4 0.0 20-21 0.011327262181201041 3.905952476276221E-4 0.0 1.9529762381381106E-4 0.0 22-23 0.011327262181201041 3.905952476276221E-4 0.0 1.9529762381381106E-4 0.0 24-25 0.011327262181201041 3.905952476276221E-4 0.0 1.9529762381381106E-4 0.0 26-27 0.011327262181201041 3.905952476276221E-4 1.9529762381381106E-4 1.9529762381381106E-4 0.0 28-29 0.011424910993107946 3.905952476276221E-4 1.9529762381381106E-4 1.9529762381381106E-4 0.0 30-31 0.011717857428828663 3.905952476276221E-4 1.9529762381381106E-4 1.9529762381381106E-4 0.0 32-33 0.01220610148836319 3.905952476276221E-4 1.9529762381381106E-4 1.9529762381381106E-4 0.0 34-35 0.012499047924083908 3.905952476276221E-4 1.9529762381381106E-4 1.9529762381381106E-4 1.9529762381381106E-4 36-37 0.01288964317171153 3.905952476276221E-4 1.9529762381381106E-4 1.9529762381381106E-4 1.9529762381381106E-4 38-39 0.013084940795525342 3.905952476276221E-4 1.9529762381381106E-4 1.9529762381381106E-4 1.9529762381381106E-4 40-41 0.013084940795525342 3.905952476276221E-4 1.9529762381381106E-4 1.9529762381381106E-4 1.9529762381381106E-4 42-43 0.013182589607432247 3.905952476276221E-4 1.9529762381381106E-4 1.9529762381381106E-4 2.929464357207166E-4 44-45 0.013280238419339152 3.905952476276221E-4 1.9529762381381106E-4 1.9529762381381106E-4 6.835416833483387E-4 46-47 0.013377887231246059 3.905952476276221E-4 1.9529762381381106E-4 1.9529762381381106E-4 7.811904952552442E-4 48-49 0.013475536043152964 3.905952476276221E-4 1.9529762381381106E-4 1.9529762381381106E-4 7.811904952552442E-4 50-51 0.013670833666966774 3.905952476276221E-4 1.9529762381381106E-4 1.9529762381381106E-4 7.811904952552442E-4 52-53 0.013866131290780584 3.905952476276221E-4 1.9529762381381106E-4 3.905952476276221E-4 7.811904952552442E-4 54-55 0.014061428914594396 4.882440595345277E-4 1.9529762381381106E-4 3.905952476276221E-4 7.811904952552442E-4 56-57 0.014061428914594396 5.858928714414332E-4 1.9529762381381106E-4 3.905952476276221E-4 7.811904952552442E-4 58-59 0.014256726538408208 5.858928714414332E-4 1.9529762381381106E-4 3.905952476276221E-4 7.811904952552442E-4 60-61 0.014452024162222018 5.858928714414332E-4 1.9529762381381106E-4 3.905952476276221E-4 7.811904952552442E-4 62-63 0.014452024162222018 5.858928714414332E-4 1.9529762381381106E-4 3.905952476276221E-4 7.811904952552442E-4 64-65 0.014452024162222018 5.858928714414332E-4 1.9529762381381106E-4 3.905952476276221E-4 7.811904952552442E-4 66-67 0.01464732178603583 5.858928714414332E-4 1.9529762381381106E-4 3.905952476276221E-4 7.811904952552442E-4 68-69 0.01464732178603583 5.858928714414332E-4 1.9529762381381106E-4 3.905952476276221E-4 7.811904952552442E-4 70-71 0.01464732178603583 5.858928714414332E-4 1.9529762381381106E-4 3.905952476276221E-4 8.788393071621497E-4 72-73 0.01464732178603583 5.858928714414332E-4 1.9529762381381106E-4 3.905952476276221E-4 9.764881190690553E-4 74-75 0.014744970597942735 5.858928714414332E-4 1.9529762381381106E-4 3.905952476276221E-4 9.764881190690553E-4 76-77 0.01484261940984964 5.858928714414332E-4 1.9529762381381106E-4 3.905952476276221E-4 9.764881190690553E-4 78-79 0.01484261940984964 5.858928714414332E-4 1.9529762381381106E-4 3.905952476276221E-4 9.764881190690553E-4 80 0.01484261940984964 5.858928714414332E-4 1.9529762381381106E-4 3.905952476276221E-4 9.764881190690553E-4 >>END_MODULE