##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-24C_S12_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1089138
Sequences flagged as poor quality	0
Sequence length	61
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.458011748740745	40.0	40.0	40.0	40.0	40.0
2	39.21309145397553	40.0	40.0	40.0	40.0	40.0
3	39.19088306532322	40.0	40.0	40.0	40.0	40.0
4	39.06311229614613	40.0	40.0	40.0	40.0	40.0
5	39.09670216262769	40.0	40.0	40.0	40.0	40.0
6	39.18854360053547	40.0	40.0	40.0	40.0	40.0
7	39.21595977736522	40.0	40.0	40.0	40.0	40.0
8	39.23873375091127	40.0	40.0	40.0	40.0	40.0
9	39.25115825542769	40.0	40.0	40.0	40.0	40.0
10	39.26227163132679	40.0	40.0	40.0	40.0	40.0
11	39.25957224887939	40.0	40.0	40.0	40.0	40.0
12	39.26614258248266	40.0	40.0	40.0	40.0	40.0
13	39.1036177233739	40.0	40.0	40.0	40.0	40.0
14	39.262051273575985	40.0	40.0	40.0	40.0	40.0
15	39.17687565763016	40.0	40.0	40.0	40.0	40.0
16	39.34450547129932	40.0	40.0	40.0	40.0	40.0
17	39.2995157638426	40.0	40.0	40.0	40.0	40.0
18	39.37563467623019	40.0	40.0	40.0	40.0	40.0
19	39.3262010874655	40.0	40.0	40.0	40.0	40.0
20	39.238796185607335	40.0	40.0	40.0	40.0	40.0
21	39.31173092849575	40.0	40.0	40.0	40.0	40.0
22	39.36644943065066	40.0	40.0	40.0	40.0	40.0
23	39.362897998233464	40.0	40.0	40.0	40.0	40.0
24	39.36504648630385	40.0	40.0	40.0	40.0	40.0
25	39.322047343862764	40.0	40.0	40.0	40.0	40.0
26	39.29768312188171	40.0	40.0	40.0	40.0	40.0
27	39.311653803282965	40.0	40.0	40.0	40.0	40.0
28	39.32269005396929	40.0	40.0	40.0	40.0	40.0
29	39.32554368684225	40.0	40.0	40.0	40.0	40.0
30	39.319322253011094	40.0	40.0	40.0	40.0	40.0
31	39.270123712513936	40.0	40.0	40.0	40.0	40.0
32	39.23078985399463	40.0	40.0	40.0	40.0	40.0
33	39.25841537068764	40.0	40.0	40.0	40.0	40.0
34	39.269308388835945	40.0	40.0	40.0	40.0	40.0
35	39.27910696348856	40.0	40.0	40.0	40.0	40.0
36	39.259667737238075	40.0	40.0	40.0	40.0	40.0
37	39.25726951038344	40.0	40.0	40.0	40.0	40.0
38	39.25306434997218	40.0	40.0	40.0	40.0	40.0
39	38.02739781368385	40.0	40.0	40.0	40.0	40.0
40	39.19340983419916	40.0	40.0	40.0	40.0	40.0
41	39.244510796611635	40.0	40.0	40.0	40.0	40.0
42	39.190519475034385	40.0	40.0	40.0	40.0	40.0
43	39.20812605932398	40.0	40.0	40.0	40.0	40.0
44	39.16226593875156	40.0	40.0	40.0	40.0	40.0
45	38.96880652405848	40.0	40.0	40.0	40.0	40.0
46	38.97971056009431	40.0	40.0	40.0	40.0	40.0
47	39.031931674406735	40.0	40.0	40.0	40.0	40.0
48	39.144306782060674	40.0	40.0	40.0	40.0	40.0
49	39.1203906208396	40.0	40.0	40.0	40.0	40.0
50	39.0948144312291	40.0	40.0	40.0	40.0	40.0
51	39.046056606233556	40.0	40.0	40.0	40.0	40.0
52	39.02829209888921	40.0	40.0	40.0	40.0	40.0
53	38.92920089097984	40.0	40.0	40.0	40.0	40.0
54	38.79433460222671	40.0	40.0	40.0	40.0	40.0
55	38.80879007067975	40.0	40.0	40.0	40.0	40.0
56	38.787183993212984	40.0	40.0	40.0	40.0	40.0
57	38.64531767324251	40.0	40.0	40.0	40.0	40.0
58	38.542348168918906	40.0	40.0	40.0	40.0	40.0
59	38.45870403934121	40.0	40.0	40.0	40.0	40.0
60	38.35812174398469	40.0	40.0	40.0	40.0	40.0
61	38.307850795766925	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.06045282303815469
1101	2	0.11487722573497194
1101	3	0.05844498888740901
1101	4	-0.09836219867079876
1101	5	-0.0036788011827511014
1101	6	0.07395151645386022
1101	7	-0.013273064506925891
1101	8	-0.014459770861037669
1101	9	-0.008868778888093232
1101	10	0.050817364544009536
1101	11	0.008132689689013262
1101	12	-0.018666075263510606
1101	13	0.0800519921427778
1101	14	0.032389107740726786
1101	15	-0.004384371405578236
1101	16	-0.052807398910303505
1101	17	0.07512401851710138
1101	18	0.0646780487668508
1101	19	0.056808918642936135
1101	20	0.04462426800145636
1101	21	0.014811924806458876
1101	22	-0.05272657298301198
1101	23	0.0103611757149622
1101	24	0.04964047427839802
1101	25	0.09077525450724266
1101	26	-0.02124964686524322
1101	27	0.009717347141815935
1101	28	0.019348096647370028
1101	29	0.08449424743981382
1101	30	0.014132977893396514
1101	31	-0.015998952693450974
1101	32	0.11674656604086664
1101	33	0.11289668448650048
1101	34	0.007940964597210609
1101	35	0.017513260016194465
1101	36	0.0023325298814000917
1101	37	-0.08431565448893252
1101	38	-0.09120067324879955
1101	39	1.131707740213578
1101	40	-0.17458839627095557
1101	41	-0.0861428259742496
1101	42	0.0040142698026315315
1101	43	0.03444787168454155
1101	44	-0.020890464171763767
1101	45	-0.049458063198514424
1101	46	0.16086737064757983
1101	47	-0.0399249757091269
1101	48	-0.0437432393330468
1101	49	-0.0018156041721155702
1101	50	0.018041140776446696
1101	51	-0.07596699060540857
1101	52	0.09319699154204386
1101	53	-0.0465400214416789
1101	54	0.05454123802646649
1101	55	0.015105298485153185
1101	56	0.012471966740129403
1101	57	0.1260792383735847
1101	58	-0.09253148396855693
1101	59	0.043793540782438356
1101	60	0.14062826185081434
1101	61	0.02762142798656697
1102	1	0.041454602850002686
1102	2	7.150085979290566E-4
1102	3	0.06254364131557821
1102	4	0.06021395185042877
1102	5	-0.016686683206650343
1102	6	-0.03907162110017026
1102	7	-0.04462826410011189
1102	8	0.034154518485514984
1102	9	-0.039318817026916975
1102	10	-0.027779126684059463
1102	11	-0.0341963212441172
1102	12	0.03198228476699683
1102	13	-0.06024803328310924
1102	14	0.007227653380191157
1102	15	0.00179920347364515
1102	16	-0.0049558860702276775
1102	17	-0.10494208878012756
1102	18	-0.0035446819730466927
1102	19	-0.06544889981625346
1102	20	-0.07789797964665013
1102	21	0.033515204745434346
1102	22	0.05296373975535573
1102	23	0.02501665499286787
1102	24	-0.07738723993467289
1102	25	-0.11743222070252557
1102	26	-0.15526439387770097
1102	27	-0.11889694220474212
1102	28	0.004275632879249258
1102	29	-0.06923064450018046
1102	30	0.06293033360150702
1102	31	-0.033878434005430336
1102	32	-0.1384886233819671
1102	33	0.03483922198967804
1102	34	-0.08434482233388252
1102	35	0.009671917529544771
1102	36	-0.050421093307001286
1102	37	0.037698075488187044
1102	38	-0.10599853747458354
1102	39	1.21895920220188
1102	40	-0.10130082952801445
1102	41	-0.015041885216561468
1102	42	-0.007620614509598056
1102	43	-0.12827778303195458
1102	44	-0.03789529508964051
1102	45	-0.059699609092234596
1102	46	-0.05203880784212345
1102	47	-0.07287692078921282
1102	48	-0.05788003058653857
1102	49	-0.0068906105285932995
1102	50	-0.045065902193300644
1102	51	-0.005710189181563408
1102	52	-0.035051165081398494
1102	53	-0.04844187753117524
1102	54	-0.0736357261230367
1102	55	-0.11622447522448454
1102	56	-0.10934852652201243
1102	57	-0.1044623329460137
1102	58	-0.09745393502372934
1102	59	-0.11111111215424785
1102	60	-0.06314493926792153
1102	61	-0.0971891491809842
1103	1	-0.0596400144860425
1103	2	-0.06819715577049834
1103	3	0.04429030697754399
1103	4	-0.0465069684519861
1103	5	-0.08629811665803544
1103	6	-0.07887972398113874
1103	7	-0.08798294724380895
1103	8	-0.0788136075034842
1103	9	-0.003055285014760045
1103	10	0.011874252865332835
1103	11	-0.013025346939244287
1103	12	-0.039511950784202554
1103	13	-0.010776755057399612
1103	14	-0.014499923039572593
1103	15	0.033589824627540565
1103	16	-0.031570308495062704
1103	17	0.02494513398630005
1103	18	-0.03720403946933715
1103	19	-0.04790086274294225
1103	20	-0.1431811786017647
1103	21	-0.0554547296980914
1103	22	-0.023586130889917456
1103	23	-0.10229452477253886
1103	24	-0.1338210680812537
1103	25	0.0043390600349226816
1103	26	-0.21353480806402558
1103	27	-0.029452790796909767
1103	28	0.0667195582302682
1103	29	-0.050482999258676386
1103	30	-0.005366956228449737
1103	31	-0.03239349190108953
1103	32	-0.1041621471053702
1103	33	-0.014722478962269747
1103	34	-0.060730425026470414
1103	35	0.024828775842152595
1103	36	0.06668839354402678
1103	37	0.026292755617788544
1103	38	-0.02218671721486487
1103	39	1.3082024142180586
1103	40	0.0334933331826619
1103	41	0.06504652811577216
1103	42	0.05179113542133251
1103	43	0.1325515943071025
1103	44	-0.015267510195258183
1103	45	0.011123488890838473
1103	46	0.06833795351722927
1103	47	0.06476656249513724
1103	48	0.11978053254610188
1103	49	0.07475508308907308
1103	50	0.0367566180890222
1103	51	-0.032483353725808684
1103	52	0.01224996771442477
1103	53	0.03861712329769773
1103	54	-0.07968601120579422
1103	55	-0.022769573559202172
1103	56	0.18982812432067675
1103	57	-0.08903219706075305
1103	58	0.03855591100646194
1103	59	-0.03945009690797008
1103	60	0.12415669587637979
1103	61	0.16429529057487002
1104	1	0.05468759532443812
1104	2	-0.02056325393842684
1104	3	0.009797685368880593
1104	4	0.017826106095334637
1104	5	0.021748866126216626
1104	6	0.09657055206282195
1104	7	0.05977489947048298
1104	8	0.10913347524060413
1104	9	0.09244373183091881
1104	10	0.08425460917946026
1104	11	0.06634588608809366
1104	12	0.08942992667347482
1104	13	0.08072253950985697
1104	14	0.03381898106309933
1104	15	0.06145167740918822
1104	16	0.06932301818092412
1104	17	0.05565662278990402
1104	18	-0.02613437620011183
1104	19	0.02437850390568741
1104	20	0.0771649834089132
1104	21	0.13318001293938408
1104	22	0.0818012359008975
1104	23	0.15753926085497483
1104	24	0.07676098784907737
1104	25	0.08220408535376578
1104	26	0.11499387549250173
1104	27	0.10034683441183745
1104	28	0.06841844359005478
1104	29	0.0954550402595089
1104	30	-0.036659135608687166
1104	31	0.12981584306535154
1104	32	-0.009308268188661373
1104	33	0.03473588090576385
1104	34	0.1062121361046664
1104	35	-0.04214796896194173
1104	36	-0.02684132482436752
1104	37	-0.12680226203713119
1104	38	0.021574363826346143
1104	39	1.241071527292931
1104	40	-0.05619811349097148
1104	41	-0.1088829232944164
1104	42	-0.005724472298929584
1104	43	0.005605591554470379
1104	44	0.06909739602885878
1104	45	0.02850500625391561
1104	46	-0.14009607880411323
1104	47	0.08733509511197468
1104	48	-0.04010869773657788
1104	49	0.09456633207543774
1104	50	0.10970570567729254
1104	51	0.03638096765519805
1104	52	-0.060516408307975667
1104	53	0.11580844124273426
1104	54	-0.058935956973684256
1104	55	-0.12764999880542405
1104	56	-0.001025068929358497
1104	57	0.017632157474096743
1104	58	0.05666362379318457
1104	59	0.03922749657473901
1104	60	-0.03793238165943791
1104	61	0.15399102266523812
1201	1	0.0931076851886985
1201	2	0.1327109375052089
1201	3	0.13133770626801322
1201	4	-0.01973692587379361
1201	5	-0.059538803287061626
1201	6	0.05164376409754112
1201	7	-0.028174993347214183
1201	8	-0.04912119710996876
1201	9	-0.022830555063876545
1201	10	0.015432326208184577
1201	11	0.009013785832344468
1201	12	0.1379518188623976
1201	13	0.08259685303751496
1201	14	0.06673828111913593
1201	15	-0.023549909549643644
1201	16	0.03258900950287824
1201	17	-0.004890779553718971
1201	18	0.07814700845568012
1201	19	0.07531804694831834
1201	20	-0.05913125157713495
1201	21	0.02809964206826976
1201	22	-0.033608551580115886
1201	23	0.03011931550242508
1201	24	0.10169710065765258
1201	25	0.008165363558163108
1201	26	-0.014644141032562175
1201	27	0.018300228217590586
1201	28	0.04643263911206219
1201	29	-4.460002581652134E-4
1201	30	6.789520374965718E-4
1201	31	0.09872546717804909
1201	32	-0.0037273795117371833
1201	33	-0.05303245447620952
1201	34	-0.043500659389238194
1201	35	-0.08865203715011205
1201	36	0.03572390152001503
1201	37	0.01830217854402605
1201	38	0.04740090905868044
1201	39	1.2088063679979726
1201	40	0.096159279651296
1201	41	0.05486853392093849
1201	42	0.1040370099573309
1201	43	0.09932994531512662
1201	44	0.002892343592471036
1201	45	0.05858346643326229
1201	46	-0.0021978664439856743
1201	47	0.02805926230902145
1201	48	-0.04618152696545508
1201	49	0.06752829145396788
1201	50	0.10706511297236432
1201	51	0.03614400850194954
1201	52	0.007920732602961778
1201	53	0.03383726148318544
1201	54	3.57898074732077E-4
1201	55	0.1341584934768889
1201	56	-0.04586908806364676
1201	57	0.15502507947081767
1201	58	0.05278149862334658
1201	59	0.1504394358451151
1201	60	0.0648838611896565
1201	61	0.0795124245823331
1202	1	0.09481986514140317
1202	2	-0.02215410761849057
1202	3	0.04602639219486804
1202	4	0.06354055016917215
1202	5	0.09720191466308847
1202	6	0.1085339564896941
1202	7	0.07864146076206424
1202	8	0.09804236802030175
1202	9	0.0010234214923769969
1202	10	-0.08060758336793583
1202	11	0.08510423501819986
1202	12	0.003715179077161679
1202	13	0.006822734259110064
1202	14	0.04920625100048426
1202	15	0.060943607175410364
1202	16	-0.0027807258018270886
1202	17	-0.07022932468025544
1202	18	0.1325465151134395
1202	19	0.08436576255995476
1202	20	0.02019975289843501
1202	21	-0.02845890286513253
1202	22	0.10601969026546954
1202	23	0.0048634957721347405
1202	24	0.005767740234453811
1202	25	0.036531210662687386
1202	26	-0.006439529201607286
1202	27	0.05693982099713679
1202	28	0.03770724753663757
1202	29	0.014163802854454843
1202	30	0.06828442075758545
1202	31	0.06463637055442462
1202	32	-0.05342987908802144
1202	33	-0.05774477860898486
1202	34	0.06288958111843357
1202	35	-0.03237652468652641
1202	36	-0.14786827333144714
1202	37	-0.13512339673611962
1202	38	-0.0892258566893318
1202	39	1.047668324404981
1202	40	0.001961411984623851
1202	41	-0.09542104159361742
1202	42	-0.2184495201944472
1202	43	-0.0040741361462792725
1202	44	-0.05080058082887007
1202	45	-0.1562088759734266
1202	46	0.05760922528417467
1202	47	0.01822038769940093
1202	48	-0.038034187587214774
1202	49	0.017197792774062748
1202	50	0.06675529564152072
1202	51	-0.09924607184264289
1202	52	0.04047079854952074
1202	53	0.010133791604992837
1202	54	-0.02281480267770064
1202	55	-0.027118735495385238
1202	56	0.15205385318314057
1202	57	-0.10668339109164293
1202	58	-0.05138443965093131
1202	59	-0.018090083942020385
1202	60	-0.10751820874334328
1202	61	-0.10643258254175691
1203	1	0.023293026198885514
1203	2	0.08149330457104753
1203	3	0.04789843796569215
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2403	10	0.011587460806040895
2403	11	-0.012672688761071527
2403	12	-0.11825352369456255
2403	13	0.0629679121883413
2403	14	-0.05571496082339422
2403	15	-0.0720539412129213
2403	16	0.02897643518006987
2403	17	0.017957150436366476
2403	18	-0.08283622385056333
2403	19	0.040311023072653995
2403	20	-0.05303516164946842
2403	21	6.851589607279607E-4
2403	22	0.020978473481129356
2403	23	-0.0015667041589111363
2403	24	0.013187306521317055
2403	25	0.017541100237956186
2403	26	-0.06327017720931138
2403	27	-0.03516187387192815
2403	28	-0.01469782575959755
2403	29	-0.03385249034685245
2403	30	0.025037118688771898
2403	31	-0.06430082376066792
2403	32	-0.027461737774885364
2403	33	-0.014305326876026925
2403	34	-0.0981945245611513
2403	35	0.07680400469261173
2403	36	-0.04343152395865957
2403	37	-0.13634715842692202
2403	38	0.09429751402461051
2403	39	1.0796401206500192
2403	40	-0.03503761909047398
2403	41	-0.16804611944149173
2403	42	-0.023704472817975386
2403	43	0.01925569488467005
2403	44	-0.17763366818338255
2403	45	0.04061847414643438
2403	46	-0.16696944228736754
2403	47	-0.20992122524031487
2403	48	-0.035190781386916115
2403	49	0.04868376380443351
2403	50	0.014017026651067965
2403	51	-0.13792334626097613
2403	52	-0.03158805103272044
2403	53	-0.1255068367380332
2403	54	0.03477626740514239
2403	55	-0.040866975826915564
2403	56	-0.04391563726593262
2403	57	-0.004412073040342079
2403	58	0.06260596376796457
2403	59	-0.05092246538499268
2403	60	-0.03572850496725977
2403	61	0.017635457518039743
2404	1	-0.10982415447527671
2404	2	-0.19200022883926948
2404	3	-0.06758098442335125
2404	4	0.08680623546142385
2404	5	-0.0283668701879094
2404	6	-0.034401866513071866
2404	7	0.016900171211361226
2404	8	0.11344071411636492
2404	9	-0.022061265696635246
2404	10	0.031016243141330335
2404	11	0.036352936204849584
2404	12	0.04705679577517685
2404	13	0.010136080133996472
2404	14	0.06666176210569574
2404	15	7.428276003693668E-4
2404	16	-0.03381514684595999
2404	17	-0.004265646542158663
2404	18	-0.1402061452378831
2404	19	0.03639856373860795
2404	20	0.11772058540866936
2404	21	-0.039058773127308655
2404	22	0.039348228499797244
2404	23	-0.03602248994319979
2404	24	-0.0012776784705508248
2404	25	0.05517536602795303
2404	26	0.08120002253092906
2404	27	0.07479312270982774
2404	28	-0.04853497182428157
2404	29	-0.008779057462618312
2404	30	0.008563826448963141
2404	31	0.05452343921923131
2404	32	0.05604763409259306
2404	33	-0.0853713025302838
2404	34	0.1657248691299813
2404	35	-0.0034877416049852172
2404	36	-0.025958621776439372
2404	37	-0.04379026803869834
2404	38	0.01877733238919177
2404	39	1.1722179818762015
2404	40	0.014596160176076012
2404	41	0.07437746632015063
2404	42	0.051837378683330826
2404	43	0.00602798933201143
2404	44	0.08429559298603806
2404	45	0.052968200616803074
2404	46	-0.09301158807943466
2404	47	0.13246125767894057
2404	48	0.1293093647099255
2404	49	-0.09817431535068977
2404	50	0.05710161322497953
2404	51	0.029305804881438746
2404	52	-0.13362236211972345
2404	53	0.09221170385941235
2404	54	-0.09401054165817868
2404	55	0.0782005488732338
2404	56	0.034192345772858346
2404	57	-0.06075512239590353
2404	58	0.050352703152178435
2404	59	-0.009598031143255525
2404	60	-0.011516274494951517
2404	61	-0.14313467553493808
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	9.0
17	30.0
18	78.0
19	131.0
20	195.0
21	263.0
22	338.0
23	565.0
24	845.0
25	1130.0
26	1369.0
27	1948.0
28	2580.0
29	3040.0
30	4055.0
31	4914.0
32	6768.0
33	8113.0
34	10392.0
35	13380.0
36	20181.0
37	31724.0
38	84785.0
39	532401.0
40	359902.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	27.429765557716284	14.466210893385412	29.229996566091714	28.87402698280659
2	29.54224821723141	17.403623952474202	28.080947429910836	24.97318040038356
3	26.61242193367599	21.576788249055674	22.020533669746168	29.79025614752217
4	26.196221231836553	20.858789244338183	23.117639821583673	29.82734970224159
5	26.897601589513908	20.896341877705122	21.077769759204067	31.128286773576903
6	29.00412987151307	23.171443839072733	23.84463676779251	23.979789521621687
7	27.78949958591106	23.076506374766097	22.99267861372939	26.141315425593454
8	24.744981811303983	26.08640961935035	23.31990987367992	25.848698695665746
9	24.566859296067165	26.07842165088354	24.02955364701259	25.3251654060367
10	26.238089204490155	23.089636024085102	24.030471804307627	26.641802967117112
11	24.71431535764981	24.68135351075805	23.72601084527397	26.878320286318168
12	26.787055451191677	23.25802607199455	23.656230890851297	26.298687585962476
13	24.703940180215913	22.115562949782305	23.914324906485678	29.2661719635161
14	25.199928750993905	24.0480086086428	23.513641062932336	27.23842157743096
15	25.242806696672048	22.834112848876817	23.524567134743254	28.39851331970788
16	25.129781533653215	24.247707820312943	23.183930778285212	27.438579867748626
17	25.516693017781034	22.27835223819204	23.20734378930861	28.99761095471832
18	27.275698763609384	23.14096101687757	23.227543249799382	26.355796969713662
19	25.15117459862754	22.533599966211813	25.21526197782099	27.09996345733966
20	25.250978296597857	22.759833923708474	23.020590595498458	28.968597184195204
21	25.449392088054957	23.37472386419352	24.94725186340023	26.228632184351298
22	27.144585901878365	22.547831404284857	23.143348225844658	27.16423446799212
23	25.430018969129716	24.71798798682995	23.026558617916187	26.825434426124144
24	25.52394646041181	25.39457809754136	23.162262266122383	25.919213175924448
25	25.27319770313771	22.875705374341912	23.225798750938818	28.625298171581566
26	25.66699536697829	23.20100850397287	24.898589526763367	26.233406602285473
27	25.705007078992743	24.090152028484912	22.876072637259924	27.32876825526242
28	25.523762828952805	23.583880096002527	22.946954380436637	27.945402694608028
29	25.867979998861486	25.799026386004343	22.55315671659606	25.77983689853811
30	27.846517153932744	24.712570858789245	22.554258505350102	24.886653481927908
31	25.895157454794525	24.249911397821027	24.499925629259103	25.355005518125346
32	26.2940967994873	24.51314709430761	22.20085976249107	26.99189634371402
33	26.28867967144659	27.239890629103016	22.026409876434393	24.445019823016
34	26.358735073057776	24.892529688616136	21.956997184929733	26.791738053396358
35	26.78347463774104	25.351883783322222	21.36267396785348	26.50196761108326
36	28.877975059175238	26.074289943055884	21.14231621704504	23.90541878072384
37	28.666064355481126	26.01543606044413	20.872653419493215	24.445846164581532
38	27.115388499896248	26.302635662331124	22.469696218477363	24.11227961929526
39	27.370109688520987	28.6811414580159	20.36325320840819	23.58549564505492
40	26.872627711088953	28.523933606209685	20.292653456219504	24.31078522648186
41	27.202705258654092	26.87281134254796	21.74774913739122	24.176734261406725
42	27.33115546422951	27.363841863932763	19.751675177984794	25.553327493852933
43	27.18829018912204	29.23063927619824	19.517820514939338	24.06325001974038
44	27.330880017040997	27.6364427648287	21.000460914962108	24.032216303168195
45	27.23667707857039	27.96863207417242	19.002367009506603	25.792323837750587
46	27.010626752532733	27.901147512987336	20.81866577054515	24.269559963934782
47	29.031490958905117	27.99902308063808	18.52676153067839	24.442724429778412
48	29.067299093411485	28.388138142273984	18.36195229621958	24.182610468094953
49	28.82132475407157	28.240957527879846	18.220005178407146	24.71771253964144
50	28.751728431107903	28.3756512030615	18.022876807163097	24.849743558667498
51	28.620064674999863	28.627134486171634	18.103858280585197	24.64894255824331
52	28.325519814752585	28.514384770341316	17.931611972036603	25.228483442869496
53	28.259779752428067	28.551753772249246	17.826942040402592	25.361524434920092
54	28.118934423369673	28.62281914688497	17.80178453051863	25.456461899226728
55	27.851475203325933	28.384465513093843	17.780207834085303	25.98385144949492
56	27.626618481771825	28.48179018636757	17.650931286944356	26.240660044916254
57	27.53783267134192	28.47912753021197	17.632109062396133	26.35093073604998
58	27.096474459618523	28.407878524117237	17.65083947121485	26.844807545049388
59	27.04257862639996	28.41632557123156	17.60823697272522	26.93285882964326
60	26.85398911799974	28.448644708016797	17.61163415471685	27.08573201926661
61	26.8660169785647	28.0463999970619	17.771577155511974	27.31600586886143
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	5.5
2	4.5
3	7.0
4	10.0
5	13.0
6	12.5
7	16.0
8	20.0
9	18.5
10	17.0
11	18.0
12	38.0
13	57.0
14	55.5
15	54.0
16	73.0
17	113.0
18	134.0
19	150.5
20	233.0
21	299.0
22	383.0
23	467.0
24	576.5
25	936.5
26	1187.0
27	1542.5
28	1898.0
29	2374.5
30	3544.5
31	4238.0
32	5358.0
33	6478.0
34	7601.0
35	10571.5
36	12419.0
37	14342.0
38	16265.0
39	19396.0
40	26060.5
41	29594.0
42	33840.0
43	42086.0
44	46086.0
45	50046.0
46	54006.0
47	58144.5
48	65574.5
49	68866.0
50	72334.0
51	75802.0
52	77953.5
53	80998.5
54	81892.0
55	81569.5
56	81247.0
57	79574.5
58	74526.5
59	71151.0
60	66998.0
61	64985.0
62	67125.0
63	51879.5
64	36634.0
65	31608.0
66	22399.5
67	18217.0
68	15262.5
69	12308.0
70	10129.0
71	6515.0
72	5080.0
73	4024.5
74	2969.0
75	2368.5
76	1359.0
77	950.0
78	741.0
79	532.0
80	391.5
81	185.0
82	119.0
83	99.0
84	48.5
85	18.0
86	15.0
87	12.0
88	8.5
89	6.5
90	8.0
91	6.5
92	5.0
93	4.0
94	3.0
95	3.0
96	4.0
97	5.0
98	4.5
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03507360867034297
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.102086237005779
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1089138.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.22982146577033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.55544962350366	61.354019147368064
2	13.657386187927523	20.548854492137306
3	2.8490118717352946	6.429919633935192
4	0.7800650558163665	2.3473661952300566
5	0.3236973792296218	1.2175848024191103
6	0.18685833516702316	0.8434391516403736
7	0.13315624525515404	0.7012124390318296
8	0.10160055127304565	0.6114713066476055
9	0.07893170035134384	0.534421595287915
>10	0.3323835565098268	3.5053286608015077
>50	9.287684198036693E-4	0.052303495971649794
>100	3.980436084872868E-4	0.07685819064536324
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	1.326812028290956E-4	1.7772208888840162
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTAATCATCTGGGGGGGGGGGGGGG	17805	1.6347790638100959	TruSeq Adapter, Index 2 (97% over 37bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9161024590088676	0.0	0.0	0.0	0.0
2	1.91904056235298	0.0	0.0	0.0	0.0
3	1.9226213758036172	9.181572950351562E-5	0.0	0.0	0.0
4	1.9253758476887226	9.181572950351562E-5	0.0	0.0	0.0
5	1.9293239240573739	9.181572950351562E-5	0.0	0.0	0.0
6	1.935934656581627	9.181572950351562E-5	0.0	0.0	0.0
7	1.9434635464009151	9.181572950351562E-5	0.0	0.0	0.0
8	2.010029950290964	9.181572950351562E-5	0.0	0.0	0.0
9	2.0216905479379106	9.181572950351562E-5	0.0	0.0	0.0
10	2.0317902781832973	9.181572950351562E-5	0.0	0.0	0.0
11	2.0431754286417334	9.181572950351562E-5	9.181572950351562E-5	0.0	0.0
12	2.0688838329027175	9.181572950351562E-5	9.181572950351562E-5	0.0	0.0
13	2.1087318595072433	9.181572950351562E-5	9.181572950351562E-5	0.0	0.0
14	2.1556496972835397	9.181572950351562E-5	9.181572950351562E-5	0.0	0.0
15	2.221573391067064	9.181572950351562E-5	9.181572950351562E-5	0.0	0.0
16	2.3051257049152634	9.181572950351562E-5	1.8363145900703124E-4	0.0	0.0
17	2.406765717475655	9.181572950351562E-5	1.8363145900703124E-4	0.0	0.0
18	2.544397496001425	9.181572950351562E-5	1.8363145900703124E-4	9.181572950351562E-5	0.0
19	2.7357414762867514	9.181572950351562E-5	1.8363145900703124E-4	9.181572950351562E-5	0.0
20	2.984378471782272	9.181572950351562E-5	1.8363145900703124E-4	9.181572950351562E-5	0.0
21	3.3312582978465537	9.181572950351562E-5	1.8363145900703124E-4	9.181572950351562E-5	0.0
22	3.7398382941371984	9.181572950351562E-5	1.8363145900703124E-4	9.181572950351562E-5	0.0
23	4.227930712177887	9.181572950351562E-5	2.7544718851054686E-4	9.181572950351562E-5	0.0
24	4.82868103031939	9.181572950351562E-5	2.7544718851054686E-4	9.181572950351562E-5	9.181572950351562E-5
25	5.521889788070933	9.181572950351562E-5	2.7544718851054686E-4	9.181572950351562E-5	1.8363145900703124E-4
26	6.316279479735351	9.181572950351562E-5	2.7544718851054686E-4	9.181572950351562E-5	1.8363145900703124E-4
27	7.2291114624593025	9.181572950351562E-5	2.7544718851054686E-4	9.181572950351562E-5	1.8363145900703124E-4
28	8.213008819818976	9.181572950351562E-5	2.7544718851054686E-4	9.181572950351562E-5	1.8363145900703124E-4
29	9.294322666181879	9.181572950351562E-5	2.7544718851054686E-4	9.181572950351562E-5	1.8363145900703124E-4
30	10.495731486735382	9.181572950351562E-5	2.7544718851054686E-4	9.181572950351562E-5	3.672629180140625E-4
31	11.810899996143739	2.7544718851054686E-4	2.7544718851054686E-4	9.181572950351562E-5	3.672629180140625E-4
32	13.200071983531931	2.7544718851054686E-4	2.7544718851054686E-4	9.181572950351562E-5	3.672629180140625E-4
33	14.710440733864763	2.7544718851054686E-4	2.7544718851054686E-4	9.181572950351562E-5	3.672629180140625E-4
34	16.279571551079844	2.7544718851054686E-4	2.7544718851054686E-4	9.181572950351562E-5	3.672629180140625E-4
35	17.930693814741566	2.7544718851054686E-4	2.7544718851054686E-4	9.181572950351562E-5	3.672629180140625E-4
36	19.669132837161133	2.7544718851054686E-4	2.7544718851054686E-4	9.181572950351562E-5	3.672629180140625E-4
37	21.476525472437835	2.7544718851054686E-4	2.7544718851054686E-4	9.181572950351562E-5	3.672629180140625E-4
38	23.33010141965481	2.7544718851054686E-4	2.7544718851054686E-4	9.181572950351562E-5	3.672629180140625E-4
39	25.219301869919146	2.7544718851054686E-4	2.7544718851054686E-4	9.181572950351562E-5	3.672629180140625E-4
40	27.220609325907276	2.7544718851054686E-4	2.7544718851054686E-4	9.181572950351562E-5	3.672629180140625E-4
41	29.280127954400637	2.7544718851054686E-4	2.7544718851054686E-4	9.181572950351562E-5	3.672629180140625E-4
42	31.394827836325607	2.7544718851054686E-4	2.7544718851054686E-4	9.181572950351562E-5	3.672629180140625E-4
43	33.51815839682391	2.7544718851054686E-4	2.7544718851054686E-4	9.181572950351562E-5	3.672629180140625E-4
44	35.665544678452136	2.7544718851054686E-4	2.7544718851054686E-4	9.181572950351562E-5	3.672629180140625E-4
45	37.81081919830178	2.7544718851054686E-4	2.7544718851054686E-4	9.181572950351562E-5	3.672629180140625E-4
46	39.93341523296405	2.7544718851054686E-4	2.7544718851054686E-4	9.181572950351562E-5	3.672629180140625E-4
47	42.01065429725159	2.7544718851054686E-4	2.7544718851054686E-4	9.181572950351562E-5	3.672629180140625E-4
48	44.10019666929259	2.7544718851054686E-4	2.7544718851054686E-4	9.181572950351562E-5	3.672629180140625E-4
49	46.138230417082134	2.7544718851054686E-4	2.7544718851054686E-4	9.181572950351562E-5	3.672629180140625E-4
50	48.162859068364156	2.7544718851054686E-4	2.7544718851054686E-4	9.181572950351562E-5	3.672629180140625E-4
>>END_MODULE