##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-24B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 535583 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.579546027413116 40.0 40.0 40.0 40.0 40.0 2 39.37116749411389 40.0 40.0 40.0 40.0 40.0 3 39.32333923966967 40.0 40.0 40.0 40.0 40.0 4 39.17905534716375 40.0 40.0 40.0 40.0 40.0 5 39.224717737493535 40.0 40.0 40.0 40.0 40.0 6 39.35741799123572 40.0 40.0 40.0 40.0 40.0 7 39.36499851563623 40.0 40.0 40.0 40.0 40.0 8 39.379188659834234 40.0 40.0 40.0 40.0 40.0 9 39.38699323914314 40.0 40.0 40.0 40.0 40.0 10-11 39.39858061215536 40.0 40.0 40.0 40.0 40.0 12-13 39.3196610049236 40.0 40.0 40.0 40.0 40.0 14-15 39.34263410153048 40.0 40.0 40.0 40.0 40.0 16-17 39.451416493802085 40.0 40.0 40.0 40.0 40.0 18-19 39.48323975929034 40.0 40.0 40.0 40.0 40.0 20-21 39.46457971966997 40.0 40.0 40.0 40.0 40.0 22-23 39.49695938818073 40.0 40.0 40.0 40.0 40.0 24-25 39.47883334609202 40.0 40.0 40.0 40.0 40.0 26-27 39.44995640264908 40.0 40.0 40.0 40.0 40.0 28-29 39.47564429789594 40.0 40.0 40.0 40.0 40.0 30-31 39.46375028198136 40.0 40.0 40.0 40.0 40.0 32-33 39.46422956852827 40.0 40.0 40.0 40.0 40.0 34-35 39.46301884961068 40.0 40.0 40.0 40.0 40.0 36-37 39.44449739848419 40.0 40.0 40.0 40.0 40.0 38-39 39.39024619936045 40.0 40.0 40.0 40.0 40.0 40-41 39.44083877966631 40.0 40.0 40.0 40.0 40.0 42-43 39.41931064004383 40.0 40.0 40.0 40.0 40.0 44-45 39.32843871172277 40.0 40.0 40.0 40.0 40.0 46-47 39.3088547514613 40.0 40.0 40.0 40.0 40.0 48-49 39.39380799578808 40.0 40.0 40.0 40.0 40.0 50-51 39.35569846246342 40.0 40.0 40.0 40.0 40.0 52-53 39.315568548587 40.0 40.0 40.0 40.0 40.0 54-55 39.23718462400901 40.0 40.0 40.0 40.0 40.0 56-57 39.25656790697455 40.0 40.0 40.0 40.0 40.0 58-59 39.20673131949791 40.0 40.0 40.0 40.0 40.0 60-61 39.14360525366392 40.0 40.0 40.0 40.0 40.0 62-63 39.381030778288846 40.0 40.0 40.0 40.0 40.0 64-65 39.38669246453934 40.0 40.0 40.0 40.0 40.0 66-67 39.391763998478254 40.0 40.0 40.0 40.0 40.0 68-69 39.38974742543 40.0 40.0 40.0 40.0 40.0 70-71 39.38199521582457 40.0 40.0 40.0 40.0 40.0 72-73 39.38593388116296 40.0 40.0 40.0 40.0 40.0 74-75 39.38612097017557 40.0 40.0 40.0 40.0 40.0 76-77 39.36526483955875 40.0 40.0 40.0 40.0 40.0 78-79 39.37023978775193 40.0 40.0 40.0 40.0 40.0 80-81 39.341179763139095 40.0 40.0 40.0 40.0 40.0 82-83 39.357382567034065 40.0 40.0 40.0 40.0 40.0 84-85 39.35460697264459 40.0 40.0 40.0 40.0 40.0 86-87 39.33398601133928 40.0 40.0 40.0 40.0 40.0 88-89 39.36149857370788 40.0 40.0 40.0 40.0 40.0 90-91 39.37091451222507 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0032973467155557046 1101 2 -0.03098670561055883 1101 3 0.12443793679036474 1101 4 -0.07342079234678067 1101 5 -0.12007772718626342 1101 6 -0.18644752130213504 1101 7 -0.01318897080528103 1101 8 0.08069530372122813 1101 9 -0.09283380534032659 1101 10-11 -0.11930481655869585 1101 12-13 -0.02471288457358156 1101 14-15 -0.05084222842744168 1101 16-17 0.03817013250266399 1101 18-19 0.021734912622363822 1101 20-21 -0.06940834481846991 1101 22-23 -0.06529716670452501 1101 24-25 0.1041011331116195 1101 26-27 -0.10502632271138879 1101 28-29 -0.01329580526775942 1101 30-31 0.03803134412338949 1101 32-33 -0.028485442620855395 1101 34-35 0.014658215142041797 1101 36-37 -0.046891010404557676 1101 38-39 0.03092669063990172 1101 40-41 -0.1694598845169608 1101 42-43 -0.14088469359938927 1101 44-45 -0.0050957824728072865 1101 46-47 0.07120587863894912 1101 48-49 -0.002678528026784477 1101 50-51 -0.2583762044997542 1101 52-53 -0.046298545787415435 1101 54-55 -0.07249434139065158 1101 56-57 -0.08396457566539794 1101 58-59 0.05641315175523687 1101 60-61 -0.017579183467070436 1101 62-63 -0.15939524715430053 1101 64-65 0.05973355401082614 1101 66-67 -0.004991294573358118 1101 68-69 0.16656394872829594 1101 70-71 -0.08227794513290831 1101 72-73 0.039918644421021554 1101 74-75 -0.15956426903681376 1101 76-77 0.10969843546292424 1101 78-79 0.16445740597832526 1101 80-81 -0.0032334670535405508 1101 82-83 -0.16818551379509472 1101 84-85 0.27273462925658976 1101 86-87 0.48433260090816077 1101 88-89 0.5450538180933364 1101 90-91 0.8506260792599605 1102 1 0.056737482927928795 1102 2 0.06872693884420045 1102 3 -0.0018525979979671092 1102 4 0.021378371804594565 1102 5 0.05177429665089761 1102 6 -0.15080012558973266 1102 7 -0.05679240707182487 1102 8 -0.16896803502964275 1102 9 0.03495816122573103 1102 10-11 0.022781709252001292 1102 12-13 -0.08326485423537378 1102 14-15 -0.10036625907522279 1102 16-17 -0.07390323643163299 1102 18-19 -0.062337527591246555 1102 20-21 -0.042757930763826835 1102 22-23 -0.01770797082184572 1102 24-25 -0.041301699486474774 1102 26-27 -0.04816152896477632 1102 28-29 -0.06848116243826041 1102 30-31 0.01277788076915698 1102 32-33 -0.04961657460919611 1102 34-35 0.03620440412537107 1102 36-37 -0.0041362990925790655 1102 38-39 -0.0010560957752971945 1102 40-41 -0.08819062575097547 1102 42-43 -0.15421532522020698 1102 44-45 0.033919039930978556 1102 46-47 -0.2182983243581873 1102 48-49 -0.04390421083382279 1102 50-51 -0.07555117123135346 1102 52-53 -0.15186278153570498 1102 54-55 0.0056382893353372765 1102 56-57 -0.15787007689738886 1102 58-59 0.13808094283184857 1102 60-61 0.12463762755467656 1102 62-63 0.10173189370178903 1102 64-65 0.08252579782200797 1102 66-67 0.151966599183595 1102 68-69 0.012684330175510183 1102 70-71 0.01022831585444095 1102 72-73 -0.12156847186682285 1102 74-75 -0.05483731168864381 1102 76-77 0.1050081529482938 1102 78-79 0.09540160267668085 1102 80-81 0.22243931959150842 1102 82-83 -0.18533996178118173 1102 84-85 -0.10904530433057147 1102 86-87 0.22083566409246913 1102 88-89 0.761270034309554 1102 90-91 0.4506260792599619 1103 1 0.08047679836968058 1103 2 0.10454493742581406 1103 3 0.056256468479581656 1103 4 0.025618601732560364 1103 5 0.0910785406134238 1103 6 0.04693045557558406 1103 7 0.009625419810333824 1103 8 -0.06660530127577147 1103 9 -0.06821827862347618 1103 10-11 -0.0013457962535809997 1103 12-13 0.09794525363667361 1103 14-15 0.030532176966730162 1103 16-17 -0.05962232119890842 1103 18-19 -0.04426056144589552 1103 20-21 -0.05591906696628257 1103 22-23 -0.1101793231416579 1103 24-25 0.04342824713739191 1103 26-27 -0.06940000457226603 1103 28-29 0.009653285697311276 1103 30-31 -0.044412966903564666 1103 32-33 -0.04465430131718762 1103 34-35 -0.029006893294486247 1103 36-37 -0.001979083043707419 1103 38-39 0.13848529260168618 1103 40-41 -0.12305607420767473 1103 42-43 0.10434894672476958 1103 44-45 -0.03776692462101039 1103 46-47 0.03626429857494884 1103 48-49 0.07190520878558715 1103 50-51 -0.010494756619110035 1103 52-53 0.07811132488021855 1103 54-55 -0.009718928890549705 1103 56-57 0.024974665978760413 1103 58-59 0.10978229634851999 1103 60-61 -0.0024196089368118123 1103 62-63 -0.22040881926860578 1103 64-65 -0.0031006512850950685 1103 66-67 -0.17430960826908404 1103 68-69 0.12963292455356168 1103 70-71 -0.11241295070664137 1103 72-73 -0.038408367676446176 1103 74-75 -0.10620589952782211 1103 76-77 0.1485681050874419 1103 78-79 -0.19910798449820533 1103 80-81 -0.6459154227789838 1103 82-83 -0.027484169966413674 1103 84-85 -0.5776934737138859 1103 86-87 0.2704634556557437 1103 88-89 -0.6952517048208833 1103 90-91 -0.3160405874067109 1104 1 -0.056896791228552956 1104 2 0.04132967857022862 1104 3 -0.10125500675037813 1104 4 -0.09449916795820457 1104 5 -0.12180297649427274 1104 6 -0.011533783767539774 1104 7 -0.16643661043672608 1104 8 -0.12903055226784943 1104 9 0.06785326097750044 1104 10-11 -0.13213232954547038 1104 12-13 -0.018458381862480167 1104 14-15 -0.11573814470544619 1104 16-17 -0.021140526125485337 1104 18-19 -0.10729477683276656 1104 20-21 -0.008161994384309423 1104 22-23 0.08024183151335507 1104 24-25 -0.014161863134539487 1104 26-27 0.09283323061966797 1104 28-29 -0.011011271843429427 1104 30-31 -0.028051392008258347 1104 32-33 -0.022398620673975245 1104 34-35 -0.061538059860666294 1104 36-37 -0.027095168520574475 1104 38-39 -0.013287650312022947 1104 40-41 -0.054872158774479374 1104 42-43 -0.04239065024144395 1104 44-45 -0.0807438164342642 1104 46-47 -0.010576260641286694 1104 48-49 -0.07764357386485443 1104 50-51 -0.07367477403653311 1104 52-53 -0.1365104372699406 1104 54-55 -0.12075492101341467 1104 56-57 0.023005110951189067 1104 58-59 -0.26803585284616815 1104 60-61 -0.02689466753891878 1104 62-63 -0.03485084140010031 1104 64-65 -0.045890885197515274 1104 66-67 -0.10233733007289914 1104 68-69 0.03489691708612952 1104 70-71 0.2695004496508133 1104 72-73 -0.16012698819024962 1104 74-75 0.0948803910779219 1104 76-77 -0.26076386954510866 1104 78-79 -0.08486558115963305 1104 80-81 -0.012776435725484703 1104 82-83 0.2940584199229761 1104 84-85 0.0917299304872401 1104 86-87 0.5865924879138049 1104 88-89 0.761270034309554 1104 90-91 0.8506260792599605 1201 1 0.012901866489571034 1201 2 0.12132967857022692 1201 3 0.15449443396550322 1201 4 -0.08320610308124543 1201 5 -0.0012302695591444035 1201 6 0.1420948515792162 1201 7 0.0255454924715508 1201 8 -0.014739724527359499 1201 9 0.07408144889696189 1201 10-11 -0.0046155510219776374 1201 12-13 0.12745660695184569 1201 14-15 -0.05583657870992198 1201 16-17 -0.08402195789282274 1201 18-19 -0.040547573253341795 1201 20-21 0.008840242752157224 1201 22-23 -0.035534454840117746 1201 24-25 0.03864350599297239 1201 26-27 0.039911530395947636 1201 28-29 0.07119007043846182 1201 30-31 0.018077657484845133 1201 32-33 0.05340220799042328 1201 34-35 0.09611959552354676 1201 36-37 0.10767699519929863 1201 38-39 0.06397305807214337 1201 40-41 0.016905659922478833 1201 42-43 0.07352514441955549 1201 44-45 0.06515726519609188 1201 46-47 0.06148067898757148 1201 48-49 0.036084179548758755 1201 50-51 0.11711616107285039 1201 52-53 0.11649888786093499 1201 54-55 0.12756793383158538 1201 56-57 0.06338094206693512 1201 58-59 -0.039980727544943306 1201 60-61 0.04852168029318449 1201 62-63 -0.13364521047581235 1201 64-65 0.13311469650733443 1201 66-67 -0.03801771059298176 1201 68-69 0.15482051995434887 1201 70-71 0.21690630506453346 1201 72-73 0.16440101566391263 1201 74-75 0.17815714483522527 1201 76-77 0.2323783537634867 1201 78-79 0.27291785442377403 1201 80-81 -0.01429867351517089 1201 82-83 -0.20456273134877279 1201 84-85 0.20862096514292716 1201 86-87 0.15643494852896112 1201 88-89 0.761270034309554 1201 90-91 0.8506260792599605 1202 1 0.043671097258801694 1202 2 0.09283469529263755 1202 3 0.06985675280608206 1202 4 0.0636746104081638 1202 5 -0.02787018037297173 1202 6 0.028079243998391235 1202 7 0.19208283918280955 1202 8 0.009911334558481144 1202 9 -0.005276410634813544 1202 10-11 -0.10695668814572201 1202 12-13 -0.18204618011616702 1202 14-15 0.0126405673324399 1202 16-17 -0.0339572979151157 1202 18-19 -0.09454534359894495 1202 20-21 -0.06518428344628546 1202 22-23 -0.09757235896497463 1202 24-25 0.05179400766521525 1202 26-27 0.02748789605927726 1202 28-29 -0.030642705481277233 1202 30-31 -0.0690336370757052 1202 32-33 -0.06210069226526116 1202 34-35 -0.0034888558488077592 1202 36-37 -0.24580329107679688 1202 38-39 -0.27464260837709276 1202 40-41 -0.08545481609115058 1202 42-43 -0.24275780042920303 1202 44-45 -0.11073231722592425 1202 46-47 -0.11658779458475976 1202 48-49 -0.20539141022868534 1202 50-51 -0.1300266046932137 1202 52-53 -0.09983661085789919 1202 54-55 -0.10452370482040862 1202 56-57 -0.007044519268539773 1202 58-59 0.03081972471044736 1202 60-61 -0.05346650296697675 1202 62-63 -0.1502557054671243 1202 64-65 -0.13337267017654852 1202 66-67 -0.35900013954284304 1202 68-69 -0.17605225597494467 1202 70-71 -0.04176625558475422 1202 72-73 -0.1276601467065177 1202 74-75 -0.06782432467565513 1202 76-77 -0.09953860588827723 1202 78-79 -0.5233191997753224 1202 80-81 -0.3534831938781977 1202 82-83 -0.35197013875618666 1202 84-85 -0.07489905750753678 1202 86-87 -0.46806589717935765 1202 88-89 -0.8988925673164587 1202 90-91 -0.09055039132827147 1203 1 0.14517489498526004 1203 2 0.24371536445492126 1203 3 0.04883505689459611 1203 4 -0.01697416139801078 1203 5 0.016588153237407255 1203 6 0.07610147848445337 1203 7 -0.026510173532422243 1203 8 -0.14352435010721365 1203 9 -0.03675221578163246 1203 10-11 0.014867550369672244 1203 12-13 0.006099416759660414 1203 14-15 -0.03428058136069012 1203 16-17 -0.07098285915193259 1203 18-19 0.014394772671110445 1203 20-21 -0.03171641728760477 1203 22-23 -0.11310635676191794 1203 24-25 -0.02085815073333208 1203 26-27 0.039004969759766084 1203 28-29 -0.026866920946567063 1203 30-31 -0.03442656379632325 1203 32-33 0.015600456283571873 1203 34-35 -0.054641246958475165 1203 36-37 -0.0032883940444961013 1203 38-39 0.11482276856520457 1203 40-41 -0.033046721594494954 1203 42-43 0.02950461314731001 1203 44-45 0.015270504827562092 1203 46-47 0.06421215936200042 1203 48-49 -0.09803756108778572 1203 50-51 0.07431035071493142 1203 52-53 -0.17541638372646418 1203 54-55 -0.10331878040456388 1203 56-57 0.02286564321208573 1203 58-59 0.06692335550052064 1203 60-61 0.21877389116737334 1203 62-63 -0.11554619654382492 1203 64-65 -0.07712297534148149 1203 66-67 0.009324061534783823 1203 68-69 -0.14961232035853556 1203 70-71 -0.3816758691422777 1203 72-73 -0.13178560332713118 1203 74-75 -0.18307964659024378 1203 76-77 -0.2599387761735059 1203 78-79 -0.138068225429528 1203 80-81 0.12226551362625315 1203 82-83 -0.24145639921019324 1203 84-85 -0.090178079924776 1203 86-87 0.13606617212433036 1203 88-89 -0.42718497417941137 1203 90-91 -0.4827072540733681 1204 1 -0.10134597976622928 1204 2 -0.0849791352137359 1204 3 -0.171178196915875 1204 4 -0.1919244596087495 1204 5 -0.023173278174127176 1204 6 0.015133950320105782 1204 7 -0.08482693960267085 1204 8 -0.013637007496214437 1204 9 -0.1189258406988003 1204 10-11 -0.20514570344079885 1204 12-13 -0.23246917170952486 1204 14-15 -0.07669277486631643 1204 16-17 -0.07893514543423663 1204 18-19 -0.11549256993988877 1204 20-21 -0.14834994942809487 1204 22-23 -0.024305826609499093 1204 24-25 -0.06194496319191245 1204 26-27 -0.019311796310475415 1204 28-29 0.06723513339404974 1204 30-31 -0.049005755313878296 1204 32-33 -0.05820150978396299 1204 34-35 -0.08335675033672629 1204 36-37 -0.06309653244835545 1204 38-39 -0.03809188692423504 1204 40-41 0.004841640262633007 1204 42-43 -0.15959840050055618 1204 44-45 -0.0833584833619625 1204 46-47 -0.010305162021523984 1204 48-49 -0.14940064539734976 1204 50-51 -0.0077184264012117865 1204 52-53 -0.028732736855140217 1204 54-55 -0.1819904013040059 1204 56-57 -0.012055509047989688 1204 58-59 0.20965270805697855 1204 60-61 0.1189658461310188 1204 62-63 -0.014938732398583454 1204 64-65 -0.07914743606484365 1204 66-67 -0.014766731251313558 1204 68-69 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-0.047406124064721666 2401 30-31 0.026713992443411883 2401 32-33 -0.03882795988128862 2401 34-35 0.05641286209850449 2401 36-37 0.0017754519906816313 2401 38-39 0.10236733522768304 2401 40-41 0.03721705879383563 2401 42-43 0.09107078355838638 2401 44-45 0.08033062286236259 2401 46-47 0.019944060243958006 2401 48-49 0.1707168646718884 2401 50-51 0.0733942705909385 2401 52-53 0.042313377938768326 2401 54-55 0.1703321698494733 2401 56-57 0.1238772161131223 2401 58-59 0.1989325647266753 2401 60-61 0.16128983761407767 2401 62-63 0.13129247299192315 2401 64-65 0.1447337897322427 2401 66-67 0.12733493860976353 2401 68-69 0.16300903031676484 2401 70-71 0.11128069200227486 2401 72-73 0.018116686217027222 2401 74-75 -0.13717245638116538 2401 76-77 0.03030134892249947 2401 78-79 0.11026825208540458 2401 80-81 -0.19338494835297837 2401 82-83 0.2594687337446331 2401 84-85 0.6432973998193603 2401 86-87 0.34746511282584436 2401 88-89 0.48540796534403796 2401 90-91 0.2791975078313911 2402 1 -0.1171443584719114 2402 2 -0.08594304870249658 2402 3 -0.03324054292201595 2402 4 -0.01820764391329277 2402 5 -0.05561958645694176 2402 6 -0.15614449099910388 2402 7 0.04330820433595761 2402 8 -0.04693695102967155 2402 9 -0.030686912684963374 2402 10-11 -4.0394342053673427E-4 2402 12-13 0.07608321198522816 2402 14-15 0.01207508025258619 2402 16-17 0.08542231534806177 2402 18-19 -0.04785933493798922 2402 20-21 -0.022798175326940395 2402 22-23 -0.026519560130303432 2402 24-25 -0.05216491722222827 2402 26-27 -0.022720210744260783 2402 28-29 0.03639602832237898 2402 30-31 -0.006652785306499709 2402 32-33 0.023242175080191885 2402 34-35 0.02119733517854172 2402 36-37 -0.05464697564073617 2402 38-39 -0.48683650005602885 2402 40-41 0.009601036317725686 2402 42-43 0.08239109291419311 2402 44-45 0.022322401193122232 2402 46-47 0.15791678342826287 2402 48-49 0.10938115496554701 2402 50-51 0.1241144128352829 2402 52-53 0.08908702620642828 2402 54-55 -0.011645113566231657 2402 56-57 0.17643080793298793 2402 58-59 0.06485467323000194 2402 60-61 0.06983780280949503 2402 62-63 0.048126996359066254 2402 64-65 0.1451893908997519 2402 66-67 0.12508115628526184 2402 68-69 0.17242628440443042 2402 70-71 0.22775798946430115 2402 72-73 0.17933781187344522 2402 74-75 0.12109192461210938 2402 76-77 0.09124400280737888 2402 78-79 -0.048524069055503105 2402 80-81 0.04853522461284143 2402 82-83 -0.4370217863741175 2402 84-85 -0.18683297198170123 2402 86-87 0.22008531088031447 2402 88-89 0.12860188944628703 2402 90-91 0.4869897156236007 2403 1 0.09442414991470827 2403 2 -0.027673826687653502 2403 3 0.09831683423256976 2403 4 0.08238495016529157 2403 5 0.07907552246221883 2403 6 0.08594128790036848 2403 7 0.07804958193908362 2403 8 0.1458905542241027 2403 9 0.18545651149084819 2403 10-11 0.11967087862249315 2403 12-13 0.10394266937881014 2403 14-15 0.10665248822380846 2403 16-17 -0.005183032838566248 2403 18-19 0.057114252819097544 2403 20-21 0.10652009586515732 2403 22-23 0.01805526307008165 2403 24-25 -0.008128652244380419 2403 26-27 0.08587514248074513 2403 28-29 0.026450961775459803 2403 30-31 -0.01954937724114103 2403 32-33 -0.02200352094591551 2403 34-35 -8.99369464200106E-4 2403 36-37 0.12204648214529357 2403 38-39 0.18162775624914218 2403 40-41 0.05989715757936409 2403 42-43 -8.937953007404076E-5 2403 44-45 0.05584808521547302 2403 46-47 -0.1318121007418256 2403 48-49 -0.012550032370775455 2403 50-51 0.01659615980021556 2403 52-53 -0.04046376592952328 2403 54-55 0.055392276249662586 2403 56-57 -1.975089452699308E-4 2403 58-59 -0.05937815040000771 2403 60-61 -0.04057121296629873 2403 62-63 0.15460917122413775 2403 64-65 -6.163466138104923E-4 2403 66-67 0.2361327199460348 2403 68-69 0.07897194099192717 2403 70-71 0.23697375966529677 2403 72-73 -0.01483031909366872 2403 74-75 0.23176118116847277 2403 76-77 0.15113841478327572 2403 78-79 0.06651195800098009 2403 80-81 0.2137366738302049 2403 82-83 0.3604544736684261 2403 84-85 0.3016235430462828 2403 86-87 -0.4200562093009239 2403 88-89 -0.48268600964649266 2403 90-91 0.8506260792599605 2404 1 0.023958134998949276 2404 2 -0.13458345074862166 2404 3 -0.03577868219112901 2404 4 0.06895710850150749 2404 5 0.10862428786105482 2404 6 -0.06812693190219221 2404 7 0.010938055814754932 2404 8 0.09139518827290516 2404 9 0.07098569371433427 2404 10-11 0.048494024496285704 2404 12-13 0.060695172269376485 2404 14-15 0.08706832421867006 2404 16-17 0.019497620915998937 2404 18-19 0.14545703352520434 2404 20-21 0.06932066394333702 2404 22-23 0.03502296536390048 2404 24-25 0.04070076166918568 2404 26-27 -0.03377323432928847 2404 28-29 -0.01585435206894914 2404 30-31 0.008879247927609413 2404 32-33 0.07510428386760992 2404 34-35 0.14390301975637954 2404 36-37 0.03813139213833949 2404 38-39 0.10952191304789949 2404 40-41 0.12918186085878602 2404 42-43 -3.514946351543813E-4 2404 44-45 -0.09290535342377382 2404 46-47 0.16220020850712302 2404 48-49 0.10782719744086222 2404 50-51 0.028457704490762126 2404 52-53 0.13402829867958843 2404 54-55 0.049747248929165266 2404 56-57 -0.15948786286270433 2404 58-59 0.11630116177674665 2404 60-61 -0.019017557903531213 2404 62-63 0.15379163763103776 2404 64-65 -0.10023146903741775 2404 66-67 0.13646262100994022 2404 68-69 0.08792040672860679 2404 70-71 0.18623966673980163 2404 72-73 -0.09697750944948069 2404 74-75 0.16794851288746315 2404 76-77 0.4299736843965434 2404 78-79 0.22288907738823838 2404 80-81 0.25319332410046513 2404 82-83 0.24595284626357738 2404 84-85 -0.5485565327649056 2404 86-87 0.03805137385545265 2404 88-89 0.20462828617844053 2404 90-91 0.12335335198723385 >>END_MODULE >>Per sequence quality scores pass #Quality Count 29 1.0 30 12.0 31 321.0 32 2240.0 33 4158.0 34 5501.0 35 6748.0 36 9493.0 37 14312.0 38 35459.0 39 217059.0 40 240279.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.541202764090723 12.664890409142934 29.337562992103933 30.456343834662412 2 28.102923793144015 17.787575419745547 27.974259714698142 26.135241072412292 3 27.206800813319315 19.686584525647753 21.45419104041764 31.652423620615295 4 26.761118258047773 20.859698683490702 20.734041222368894 31.645141836092634 5 27.33488553594868 21.261503819202627 20.90656350182885 30.497047143019852 6 27.402475433312855 23.83309403024368 23.851018422914844 24.913412113528622 7 25.97954005261556 23.699781359751896 23.30917896945945 27.011499618173097 8 25.002473939613466 24.818002065039405 23.86800925346772 26.31151474187941 9 24.581250711841115 24.95374199703874 24.55062987436121 25.91437741675893 10-11 24.414236448879073 23.410843884141208 24.52140938005874 27.65351028692098 12-13 24.76992361594748 23.21675631974876 24.39341801364121 27.61990205066255 14-15 25.23343720767836 22.92679192580795 24.190554965336837 27.64921590117685 16-17 25.286370179785393 22.66082007830719 23.93783969991579 28.114970041991622 18-19 25.228395972239596 23.0329379386575 23.86231452454615 27.876351564556757 20-21 25.422203467996557 23.048155001185624 23.823198271789806 27.706443259028013 22-23 25.18059759178316 22.564196399064198 24.003375760619736 28.251830248532904 24-25 25.25005461338392 22.916336030083105 24.09533162927128 27.738277727261696 26-27 25.512945705894325 23.051515824811467 23.915620921500498 27.519917547793714 28-29 25.52610893176221 22.700496468334507 23.9162744149833 27.857120184919985 30-31 25.619469770099272 22.973641610294653 23.980495874558187 27.426392745047885 32-33 25.690174723293342 22.945658345917074 24.019449660406554 27.34471727038303 34-35 25.597217030279413 22.62803330973761 24.07638651385122 27.69836314613176 36-37 25.6917331962494 23.02761336112323 24.136998255703684 27.143655186923688 38-39 25.97764391566252 22.917921171107523 24.056159770787094 27.04827514244287 40-41 25.617995025809908 22.71134582529875 24.22261103757208 27.448048111319263 42-43 25.73716941027661 23.050936339590162 24.197098057400343 27.01479619273288 44-45 25.89451484981983 23.04888699128612 24.10386787509461 26.952730283799433 46-47 25.772864499842747 22.793077506051155 24.223741034106762 27.210316959999336 48-49 25.840131430711633 23.057375593698843 24.23723780632473 26.865255169264792 50-51 25.989948353328458 23.127666891077027 24.15866296631746 26.72372178927706 52-53 25.933904262304292 22.92669566957749 24.135267328888094 27.004132739230123 54-55 26.065091764850294 23.119103468249115 24.197573011985337 26.618231754915257 56-57 26.14603056923994 23.096267620911096 24.124930759007647 26.63277105084132 58-59 26.035843892737827 22.952126053036324 24.154899145489775 26.857130908736078 60-61 26.18796688303155 23.225941109928648 24.107462655799463 26.47862935124034 62-63 26.42467519016767 23.226923829361198 23.941562999873415 26.406837980597718 64-65 26.291142022390567 23.016586084753214 23.853646870232957 26.838625022623262 66-67 26.34331819958288 23.19335527301018 23.91838732417516 26.54493920323178 68-69 26.564351126316183 23.059655890556733 24.011173788155286 26.364819194971794 70-71 26.495167641092948 22.89321083402935 23.73797876148431 26.87364276339339 72-73 26.586815731594488 23.110419217409127 23.678830822756407 26.623934228239975 74-75 26.711477521911988 23.28949851317322 23.46713689868105 26.53188706623374 76-77 26.65663234826326 23.004709405621803 23.411254470831942 26.927403775282993 78-79 26.851305122076948 23.042316884577534 23.557804958058014 26.548573035287504 80-81 27.127820247693847 23.139391686223483 22.97093198875386 26.76185607732881 82-83 27.820409862883317 22.523125427560156 22.61681687040838 27.039647839148152 84-85 28.25835757261824 21.960792126548988 22.15603458580707 27.6248157150257 86-87 28.362422997946613 21.415126625598905 21.691763632215377 28.5306867442391 88-89 27.417299797083665 20.26331933367939 22.047095465055918 30.27228540418102 90-91 23.869061150261423 22.539213457604003 24.96021823141623 28.631507160718346 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 0.5 7 1.0 8 2.5 9 5.0 10 7.5 11 8.0 12 12.0 13 19.0 14 30.0 15 48.0 16 84.5 17 128.0 18 167.5 19 220.0 20 283.0 21 381.0 22 493.0 23 638.5 24 844.5 25 1084.5 26 1389.0 27 1759.5 28 2229.5 29 2768.0 30 3365.5 31 4097.0 32 4978.5 33 5866.0 34 6989.0 35 8365.5 36 9799.5 37 11333.5 38 13073.0 39 15092.0 40 17228.5 41 19391.5 42 21441.5 43 23630.0 44 25785.0 45 27645.5 46 29458.5 47 31169.0 48 32621.0 49 33929.0 50 35131.5 51 35914.0 52 36249.5 53 36558.0 54 36912.0 55 37027.0 56 36673.5 57 36178.5 58 35485.0 59 34596.5 60 33870.5 61 32865.0 62 31573.5 63 29693.0 64 27603.0 65 25681.0 66 23233.5 67 20927.5 68 18694.0 69 16264.5 70 13944.5 71 11710.0 72 9667.5 73 7677.0 74 5914.5 75 4651.0 76 3626.0 77 2678.0 78 2040.0 79 1556.5 80 1078.0 81 706.0 82 483.0 83 342.0 84 251.5 85 175.5 86 105.5 87 63.5 88 38.5 89 22.0 90 14.5 91 11.5 92 10.0 93 8.5 94 7.5 95 5.5 96 2.5 97 2.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014936993892636623 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.06376241710614215 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 4.7726773765547E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 8820.0 32-33 18879.0 34-35 20262.0 36-37 21466.0 38-39 23395.0 40-41 24575.0 42-43 25345.0 44-45 25489.0 46-47 25927.0 48-49 25398.0 50-51 25114.0 52-53 24348.0 54-55 23467.0 56-57 22480.0 58-59 21426.0 60-61 20462.0 62-63 19484.0 64-65 18092.0 66-67 16834.0 68-69 15760.0 70-71 14820.0 72-73 13627.0 74-75 12759.0 76-77 11344.0 78-79 10505.0 80-81 9647.0 82-83 8736.0 84-85 7769.0 86-87 7106.0 88-89 6349.0 90-91 5898.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.9067386105375 #Duplication Level Percentage of deduplicated Percentage of total 1 99.90933809582565 99.81616125891469 2 0.08830757997601583 0.176450446199859 3 0.002022105049254519 0.006060657617967579 4 3.3221914906371225E-4 0.00132763726747694 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.00168041181292162 0.0 0.0 0.0 0.0 2 0.00168041181292162 0.0 0.0 0.0 1.8671242365795777E-4 3 0.00168041181292162 0.0 0.0 0.0 1.8671242365795777E-4 4 0.00168041181292162 0.0 0.0 0.0 1.8671242365795777E-4 5 0.0018671242365795777 0.0 0.0 0.0 1.8671242365795777E-4 6 0.0020538366602375355 0.0 0.0 0.0 1.8671242365795777E-4 7 0.0028006863548693667 0.0 0.0 0.0 1.8671242365795777E-4 8 0.009335621182897888 0.0 0.0 0.0 1.8671242365795777E-4 9 0.009709046030213804 0.0 0.0 0.0 3.7342484731591553E-4 10-11 0.009709046030213804 0.0 0.0 0.0 3.7342484731591553E-4 12-13 0.009709046030213804 0.0 0.0 0.0 3.7342484731591553E-4 14-15 0.009709046030213804 0.0 0.0 0.0 3.7342484731591553E-4 16-17 0.009709046030213804 0.0 0.0 0.0 3.7342484731591553E-4 18-19 0.009802402242042783 9.335621182897888E-5 0.0 0.0 3.7342484731591553E-4 20-21 0.0101758270893587 1.8671242365795777E-4 0.0 0.0 3.7342484731591553E-4 22-23 0.010455895724845635 1.8671242365795777E-4 0.0 0.0 3.7342484731591553E-4 24-25 0.01082932057216155 1.8671242365795777E-4 0.0 0.0 4.667810591448945E-4 26-27 0.01092267678399053 1.8671242365795777E-4 0.0 0.0 5.601372709738734E-4 28-29 0.011202745419477467 1.8671242365795777E-4 0.0 0.0 5.601372709738734E-4 30-31 0.01166952647862236 1.8671242365795777E-4 0.0 0.0 5.601372709738734E-4 32-33 0.012136307537767255 1.8671242365795777E-4 0.0 0.0 5.601372709738734E-4 34-35 0.012323019961425213 1.8671242365795777E-4 9.335621182897888E-5 0.0 5.601372709738734E-4 36-37 0.012323019961425213 1.8671242365795777E-4 1.8671242365795777E-4 0.0 7.468496946318311E-4 38-39 0.012323019961425213 1.8671242365795777E-4 1.8671242365795777E-4 0.0 7.468496946318311E-4 40-41 0.01260308859691215 1.8671242365795777E-4 1.8671242365795777E-4 0.0 7.468496946318311E-4 42-43 0.012976513444228066 1.8671242365795777E-4 1.8671242365795777E-4 0.0 9.335621182897889E-4 44-45 0.013069869656057045 1.8671242365795777E-4 1.8671242365795777E-4 0.0 9.335621182897889E-4 46-47 0.013069869656057045 1.8671242365795777E-4 1.8671242365795777E-4 0.0 9.335621182897889E-4 48-49 0.013069869656057045 1.8671242365795777E-4 1.8671242365795777E-4 0.0 9.335621182897889E-4 50-51 0.013163225867886024 1.8671242365795777E-4 1.8671242365795777E-4 0.0 0.0011202745419477468 52-53 0.013256582079715001 1.8671242365795777E-4 1.8671242365795777E-4 0.0 0.0011202745419477468 54-55 0.013256582079715001 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