##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-24A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 388127 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.58060119496969 40.0 40.0 40.0 40.0 40.0 2 39.362878645391845 40.0 40.0 40.0 40.0 40.0 3 39.3321000600319 40.0 40.0 40.0 40.0 40.0 4 39.23178753346198 40.0 40.0 40.0 40.0 40.0 5 39.27548457077194 40.0 40.0 40.0 40.0 40.0 6 39.38335647867837 40.0 40.0 40.0 40.0 40.0 7 39.41116181043834 40.0 40.0 40.0 40.0 40.0 8 39.415933444465345 40.0 40.0 40.0 40.0 40.0 9 39.41746902431421 40.0 40.0 40.0 40.0 40.0 10-11 39.422080916813314 40.0 40.0 40.0 40.0 40.0 12-13 39.33431067665996 40.0 40.0 40.0 40.0 40.0 14-15 39.369711460424035 40.0 40.0 40.0 40.0 40.0 16-17 39.470224952141955 40.0 40.0 40.0 40.0 40.0 18-19 39.51547818111082 40.0 40.0 40.0 40.0 40.0 20-21 39.49551564307559 40.0 40.0 40.0 40.0 40.0 22-23 39.53793474816233 40.0 40.0 40.0 40.0 40.0 24-25 39.50849077750324 40.0 40.0 40.0 40.0 40.0 26-27 39.491563328498145 40.0 40.0 40.0 40.0 40.0 28-29 39.49648439814803 40.0 40.0 40.0 40.0 40.0 30-31 39.45680379448804 40.0 40.0 40.0 40.0 40.0 32-33 39.46073289904618 40.0 40.0 40.0 40.0 40.0 34-35 39.45707396546838 40.0 40.0 40.0 40.0 40.0 36-37 39.44134946076849 40.0 40.0 40.0 40.0 40.0 38-39 39.3822393291028 40.0 40.0 40.0 40.0 40.0 40-41 39.42486472054735 40.0 40.0 40.0 40.0 40.0 42-43 39.405224109455645 40.0 40.0 40.0 40.0 40.0 44-45 39.33592669668936 40.0 40.0 40.0 40.0 40.0 46-47 39.281646295934635 40.0 40.0 40.0 40.0 40.0 48-49 39.35957386216346 40.0 40.0 40.0 40.0 40.0 50-51 39.30652508524416 40.0 40.0 40.0 40.0 40.0 52-53 39.26552028021129 40.0 40.0 40.0 40.0 40.0 54-55 39.16074458428881 40.0 40.0 40.0 40.0 40.0 56-57 39.17378193195203 40.0 40.0 40.0 40.0 40.0 58-59 39.13281273866966 40.0 40.0 40.0 40.0 40.0 60-61 39.06089544164165 40.0 40.0 40.0 40.0 40.0 62-63 39.41913981972548 40.0 40.0 40.0 40.0 40.0 64-65 39.42403815489179 40.0 40.0 40.0 40.0 40.0 66-67 39.41967603161868 40.0 40.0 40.0 40.0 40.0 68-69 39.42328084593133 40.0 40.0 40.0 40.0 40.0 70-71 39.41445363237858 40.0 40.0 40.0 40.0 40.0 72-73 39.41170315338552 40.0 40.0 40.0 40.0 40.0 74-75 39.40592749069802 40.0 40.0 40.0 40.0 40.0 76-77 39.39884178492923 40.0 40.0 40.0 40.0 40.0 78-79 39.387768620938665 40.0 40.0 40.0 40.0 40.0 80-81 39.403885402593104 40.0 40.0 40.0 40.0 40.0 82-83 39.429225214519136 40.0 40.0 40.0 40.0 40.0 84-85 39.40931752733285 40.0 40.0 40.0 40.0 40.0 86-87 39.38302371461973 40.0 40.0 40.0 40.0 40.0 88-89 39.36808076748997 40.0 40.0 40.0 40.0 40.0 90-91 39.503610057592354 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14597288619921045 1101 2 -0.08382310925998127 1101 3 0.09334100619052066 1101 4 0.06712412672160895 1101 5 0.21779925478973183 1101 6 0.234638694585378 1101 7 0.015565852531700841 1101 8 0.0015277276128458084 1101 9 -0.03852028179289846 1101 10-11 0.05008649309478841 1101 12-13 0.10945215238797346 1101 14-15 0.017232044026400217 1101 16-17 0.12011651258330147 1101 18-19 0.054268221720612075 1101 20-21 -0.04234262486006202 1101 22-23 0.0660620271691954 1101 24-25 -0.04365962225862319 1101 26-27 0.04617889290684474 1101 28-29 0.02274977655783772 1101 30-31 0.0636262337339275 1101 32-33 0.02001847925313882 1101 34-35 -0.012199146440586617 1101 36-37 0.0280443258410088 1101 38-39 0.1347413544823297 1101 40-41 -0.026606167648346002 1101 42-43 -0.0516516763105912 1101 44-45 -0.011526212967673644 1101 46-47 0.021909523491459026 1101 48-49 -0.027661675755247472 1101 50-51 0.00870706127890486 1101 52-53 0.002868514188364202 1101 54-55 0.12145537432220976 1101 56-57 0.27607199073477773 1101 58-59 0.17529648702723932 1101 60-61 0.18383319678620325 1101 62-63 0.18090213498667396 1101 64-65 -0.038934100130987304 1101 66-67 0.12337583066400981 1101 68-69 0.3210701336648043 1101 70-71 0.250515398571423 1101 72-73 0.16297952811729743 1101 74-75 0.3468033312432084 1101 76-77 0.2015205919163776 1101 78-79 0.3378460035965034 1101 80-81 -0.20146934693809015 1101 82-83 0.09909178073308311 1101 84-85 -0.7723811824309337 1101 86-87 -0.6700371452268357 1101 88-89 0.0203109982930485 1101 90-91 0.334531266153121 1102 1 0.04779223664361609 1102 2 0.13485581112124834 1102 3 -0.04399908715132028 1102 4 -0.033688897007174035 1102 5 -0.017878816593274394 1102 6 -0.1269175143737158 1102 7 0.006939646786335629 1102 8 -0.07321865215317302 1102 9 -0.01566544130383818 1102 10-11 -0.2202997771168853 1102 12-13 -0.02227591875519863 1102 14-15 -0.10777886823898086 1102 16-17 -0.04862212547004674 1102 18-19 0.03202350877315041 1102 20-21 0.006878167442359029 1102 22-23 -0.013095306112973049 1102 24-25 0.011380405369791902 1102 26-27 -0.1705775501263318 1102 28-29 0.006922914637570443 1102 30-31 0.07059281582053956 1102 32-33 0.05264252859004159 1102 34-35 0.009564645709744468 1102 36-37 -0.20722588914139095 1102 38-39 -0.01623143821844053 1102 40-41 0.05441958322117557 1102 42-43 -0.08962570425433825 1102 44-45 -0.0632490019309131 1102 46-47 -0.007182355481674563 1102 48-49 -0.10273957136649159 1102 50-51 0.03137046326267523 1102 52-53 -0.1203187743956633 1102 54-55 0.021701658254038136 1102 56-57 -0.3172053519613698 1102 58-59 -0.1693648770840923 1102 60-61 -0.008264546813805396 1102 62-63 0.005903685877143516 1102 64-65 0.0015143855298802578 1102 66-67 -0.08374214194247287 1102 68-69 0.08931270408548642 1102 70-71 0.2064638386951856 1102 72-73 -0.3439986705255862 1102 74-75 0.025024302482954397 1102 76-77 -0.1426384085792307 1102 78-79 0.035526385230376434 1102 80-81 0.18018823470448808 1102 82-83 0.2912296528310705 1102 84-85 -0.12280671434582757 1102 86-87 -0.24146571665541217 1102 88-89 -0.6353666573846084 1102 90-91 -0.7423918107699592 1103 1 0.08940160804330333 1103 2 -0.17007889085664374 1103 3 -0.22429521386739282 1103 4 -0.1816556273490164 1103 5 -0.06809176916394222 1103 6 -0.0011677206366726978 1103 7 -0.0038640726365741784 1103 8 0.09684980485121741 1103 9 -0.018913618385234088 1103 10-11 -0.03640055236829909 1103 12-13 0.02783668967222752 1103 14-15 -0.08492641872695117 1103 16-17 -0.016391215014188276 1103 18-19 -0.0963277997888099 1103 20-21 -0.08622447346683515 1103 22-23 0.005890177434302757 1103 24-25 -0.06470275390981328 1103 26-27 -0.06753712258596067 1103 28-29 0.03782705253271956 1103 30-31 -0.05643543663045847 1103 32-33 0.0653877633387765 1103 34-35 0.07630271691321866 1103 36-37 -0.032430060351991585 1103 38-39 0.014926275262098443 1103 40-41 -0.10287945073206117 1103 42-43 -0.01574695818892735 1103 44-45 0.06494806141753173 1103 46-47 0.022714224745499223 1103 48-49 0.05222743279747277 1103 50-51 0.014474523147214313 1103 52-53 0.06825530978441208 1103 54-55 -0.12329815197531246 1103 56-57 -0.02906704237708624 1103 58-59 0.21318217925214356 1103 60-61 0.0021435755318748306 1103 62-63 -0.2572751167680778 1103 64-65 -0.24440405190077286 1103 66-67 -0.04090038216509129 1103 68-69 -0.1281946673188301 1103 70-71 -0.12064150567417897 1103 72-73 -0.048363851832284865 1103 74-75 0.04719976695879069 1103 76-77 -0.610943016188088 1103 78-79 -0.00923577088179428 1103 80-81 -0.2133876231915579 1103 82-83 -1.0770732307260502 1103 84-85 -0.594904901596017 1103 86-87 0.03228843616851407 1103 88-89 -1.8156678376857869 1103 90-91 -0.19880206718021043 1104 1 0.0392257782122698 1104 2 0.017215616962779734 1104 3 -0.17753591489950082 1104 4 -0.20498524395421214 1104 5 0.021467083796572695 1104 6 -0.03064423377387726 1104 7 -0.014828420636689543 1104 8 -0.024730090508590763 1104 9 -0.007620482896200542 1104 10-11 0.0012885004378304643 1104 12-13 -0.06315167410969025 1104 14-15 0.03373727455720399 1104 16-17 -0.01398248928013146 1104 18-19 -0.06486426660985956 1104 20-21 -0.04521895555506461 1104 22-23 -0.008111881061282133 1104 24-25 -0.04850639630338094 1104 26-27 0.07409166204394069 1104 28-29 0.0596199450049113 1104 30-31 -0.05925157294860384 1104 32-33 -0.03898782645818244 1104 34-35 0.04638954852065069 1104 36-37 -0.008136649388269745 1104 38-39 -0.12383053175442882 1104 40-41 -0.10980944938020087 1104 42-43 0.09238807675722427 1104 44-45 -0.09927617262435007 1104 46-47 -5.90772619077029E-4 1104 48-49 -0.002694034159098635 1104 50-51 -0.034252342478183095 1104 52-53 -0.0081460658897754 1104 54-55 0.08667616685625745 1104 56-57 0.2744745415848371 1104 58-59 0.003687456190021976 1104 60-61 -0.076520111344621 1104 62-63 -0.12328140277475086 1104 64-65 0.004785271528724877 1104 66-67 -0.0453846703845997 1104 68-69 -0.2096638239355002 1104 70-71 -0.222695658540502 1104 72-73 -0.022092287548538536 1104 74-75 -0.1411890470458843 1104 76-77 0.02524646482898163 1104 78-79 0.17287387427655432 1104 80-81 -0.03987539014512009 1104 82-83 -0.29885402609097156 1104 84-85 -0.36891316896291926 1104 86-87 -0.06368793887763502 1104 88-89 0.5917395697216179 1104 90-91 0.334531266153121 1201 1 -0.04606247128893415 1201 2 0.25013941548565555 1201 3 0.1941466781209087 1201 4 0.0622624721016507 1201 5 -0.11929388088542225 1201 6 0.09394535634127976 1201 7 0.07979862266198978 1201 8 -0.0011240839980359851 1201 9 -0.11866392158848527 1201 10-11 -0.0013752259789185928 1201 12-13 -0.052437263384774724 1201 14-15 0.04797648489008566 1201 16-17 0.09354315039923478 1201 18-19 -2.872775657962734E-4 1201 20-21 -0.0983989758567887 1201 22-23 -0.059576755575612594 1201 24-25 0.05345377031078158 1201 26-27 0.22067362151049963 1201 28-29 -0.01245118104080234 1201 30-31 -0.05448066742163604 1201 32-33 -0.04938972052891444 1201 34-35 -0.03518221766971408 1201 36-37 0.07011130023920487 1201 38-39 0.0814424254500068 1201 40-41 -0.08543226814451543 1201 42-43 0.0875616983053007 1201 44-45 0.06828220946167107 1201 46-47 0.06241921826942587 1201 48-49 -0.15627538393749063 1201 50-51 -0.01866401121664296 1201 52-53 0.009695633217958743 1201 54-55 0.12398127608593512 1201 56-57 -0.032932970503651404 1201 58-59 0.0634937676684757 1201 60-61 0.2671653826415721 1201 62-63 -0.09033848956221391 1201 64-65 -0.08710960093961262 1201 66-67 0.01500820234060285 1201 68-69 0.033923240349793105 1201 70-71 0.05655498426843053 1201 72-73 0.1906473961305224 1201 74-75 0.1883102088797841 1201 76-77 -0.07451930264838325 1201 78-79 0.11639034802741577 1201 80-81 0.14018620774593415 1201 82-83 -0.10895246260528069 1201 84-85 -0.36608615670194666 1201 86-87 0.2924628547731629 1201 88-89 0.2107871887692383 1201 90-91 -0.665468733846879 1202 1 -0.03653453558658981 1202 2 -0.0989211420789573 1202 3 -0.20908942476241066 1202 4 -0.16217806229741427 1202 5 -0.11062536922749189 1202 6 -0.17369980409338126 1202 7 -0.002416074196510465 1202 8 -0.13723846166715958 1202 9 -0.040268887149629506 1202 10-11 -0.1447133633629818 1202 12-13 -0.029144396073832013 1202 14-15 -0.1486636268704089 1202 16-17 -0.008105847381465026 1202 18-19 -0.05543719047395257 1202 20-21 0.007727515093613135 1202 22-23 -0.10000328454580654 1202 24-25 -0.008804958767285598 1202 26-27 0.08295180257310619 1202 28-29 -0.13040978368096035 1202 30-31 -0.08770642538589613 1202 32-33 -0.030490567249813694 1202 34-35 -0.04371265780228839 1202 36-37 -0.24812918536449757 1202 38-39 -0.003421232742994107 1202 40-41 -0.08256923389682669 1202 42-43 -0.2670740275701107 1202 44-45 -0.13751253966689347 1202 46-47 -0.14186170913441742 1202 48-49 -0.20197643956017686 1202 50-51 -0.12878549351277968 1202 52-53 -0.06755868666477483 1202 54-55 -0.12440646804884636 1202 56-57 -0.15762622864660614 1202 58-59 -0.04941202179987414 1202 60-61 -0.36031162281587115 1202 62-63 -0.22827525614549415 1202 64-65 -0.2834555444288469 1202 66-67 -0.17353278397843752 1202 68-69 -0.0014275632231885993 1202 70-71 -0.06255912422730603 1202 72-73 -0.09359824314402232 1202 74-75 -0.12352633908646027 1202 76-77 -0.13027113942212054 1202 78-79 -0.32857278796791434 1202 80-81 -0.2214778713754484 1202 82-83 0.21826301197861397 1202 84-85 0.156080356030607 1202 86-87 0.22996285477316292 1202 88-89 0.5917395697216179 1202 90-91 0.334531266153121 1203 1 0.01669133031666803 1203 2 -0.05129799890236342 1203 3 -0.17335978070731528 1203 4 0.02300666782582539 1203 5 -0.22813510641556434 1203 6 -0.1766977522190487 1203 7 -0.08323780686308879 1203 8 -0.022603805878290473 1203 9 -0.18765981221321937 1203 10-11 -0.08614279485863818 1203 12-13 -0.23632227023374242 1203 14-15 -0.11637425115624467 1203 16-17 -0.04620681925727155 1203 18-19 -0.11357772543567535 1203 20-21 -0.11063000250704391 1203 22-23 0.01662181221173853 1203 24-25 -0.11206215568579125 1203 26-27 0.11139544974901128 1203 28-29 -0.055018760324003324 1203 30-31 -0.06154809932218086 1203 32-33 0.12354102170599646 1203 34-35 -0.031263966901668994 1203 36-37 -0.07368906148479226 1203 38-39 -0.00828599595431001 1203 40-41 -0.045127848897593026 1203 42-43 -0.02471449656793112 1203 44-45 -0.05958162280211354 1203 46-47 0.041927434438925104 1203 48-49 0.01623797743408062 1203 50-51 -0.13835210850536583 1203 52-53 0.051977773819103845 1203 54-55 -0.12401834538356837 1203 56-57 -0.06091354088978562 1203 58-59 -0.05631418495693907 1203 60-61 -0.3324537633099425 1203 62-63 -0.05911526164520353 1203 64-65 0.12513946119414499 1203 66-67 -0.08523096100488914 1203 68-69 -0.05292523069846311 1203 70-71 -0.1880379119814748 1203 72-73 -0.30732161229065724 1203 74-75 -0.4214782080060786 1203 76-77 -0.014897386567142235 1203 78-79 -0.5339129408229866 1203 80-81 -0.05274308518335147 1203 82-83 -0.7753199839727998 1203 84-85 0.12904513750602575 1203 86-87 0.09487513547492199 1203 88-89 -0.5159527379706859 1203 90-91 -0.9988020671802076 1204 1 0.044047061763365036 1204 2 -0.16269672956654802 1204 3 -0.35388112839635255 1204 4 -0.036240319665836296 1204 5 -0.12736905766885798 1204 6 0.11609480354566415 1204 7 -0.10299246078321289 1204 8 -0.29213142895446964 1204 9 -0.15145030220943312 1204 10-11 -0.002030201139454846 1204 12-13 -0.15985318971289786 1204 14-15 -0.025320878963157156 1204 16-17 0.027330805058028318 1204 18-19 -0.10720897981715893 1204 20-21 0.06065225072527625 1204 22-23 -0.04868159435424957 1204 24-25 -0.19698102716850485 1204 26-27 -0.016911349973284473 1204 28-29 -0.1388716477319747 1204 30-31 -0.08687201080476115 1204 32-33 -0.14349222927577898 1204 34-35 -0.1925424026790452 1204 36-37 -0.0012986100523519895 1204 38-39 0.015174194963890386 1204 40-41 -0.06422723655343532 1204 42-43 -0.1679764975753386 1204 44-45 -0.14661043113208194 1204 46-47 -0.15616325126977415 1204 48-49 -0.021539596750898227 1204 50-51 0.006399186486994779 1204 52-53 -0.06801807928212611 1204 54-55 -0.3107849132327871 1204 56-57 -0.008843779227106552 1204 58-59 -0.05207885091287778 1204 60-61 -0.3201632498788456 1204 62-63 -0.12540739132865042 1204 64-65 -0.10294747405121996 1204 66-67 0.037646916233789796 1204 68-69 -0.14941824078586308 1204 70-71 -0.3025283925610651 1204 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2401 42-43 0.08158633388850944 2401 44-45 0.0033981964445146673 2401 46-47 0.002521639536595899 2401 48-49 0.05610543859189221 2401 50-51 0.19286676289540594 2401 52-53 -0.09832915746814308 2401 54-55 0.11249794752348663 2401 56-57 0.03424238037403171 2401 58-59 -0.20376454798118004 2401 60-61 0.1073067407212065 2401 62-63 0.06754819087890951 2401 64-65 -0.05802326081107623 2401 66-67 -0.12216649422524739 2401 68-69 -0.019442482234268255 2401 70-71 0.18266732780692507 2401 72-73 0.06240739403808249 2401 74-75 0.07744866188853905 2401 76-77 -0.18234738765713132 2401 78-79 0.06663936709575324 2401 80-81 0.19336743879359375 2401 82-83 0.1331403911028204 2401 84-85 -0.20308286880425896 2401 86-87 -0.11088595159288417 2401 88-89 0.5917395697216179 2401 90-91 0.334531266153121 2402 1 0.10338246081205682 2402 2 0.16459699797132288 2402 3 0.064916544388268 2402 4 0.15099588460746105 2402 5 0.17015208390003522 2402 6 0.16069808004655073 2402 7 0.06367841819047015 2402 8 -0.09717406592606181 2402 9 -0.06503145398896493 2402 10-11 0.016802602285657997 2402 12-13 -0.009118731862088225 2402 14-15 0.04121756507802843 2402 16-17 0.03847783310693842 2402 18-19 -0.13910330482866584 2402 20-21 0.03385692335320556 2402 22-23 -0.019720298716009665 2402 24-25 0.1341606428258828 2402 26-27 -0.005941238818927275 2402 28-29 -0.00395735133047026 2402 30-31 -0.009579546958995877 2402 32-33 -0.010437284996356766 2402 34-35 0.018892605582976785 2402 36-37 -0.11703567685719207 2402 38-39 -0.45054031361284075 2402 40-41 0.02564748612141443 2402 42-43 0.08897037210113012 2402 44-45 0.05449162399454366 2402 46-47 0.17138310148047253 2402 48-49 0.11970065677480335 2402 50-51 0.012242019101137203 2402 52-53 0.11394937135968064 2402 54-55 0.07570737800684668 2402 56-57 0.11413171259874133 2402 58-59 -0.12384766238891132 2402 60-61 0.008106037469453042 2402 62-63 0.14463918643011198 2402 64-65 0.13593079970011956 2402 66-67 0.1384196572722729 2402 68-69 -0.0277297209058176 2402 70-71 0.12896541430662012 2402 72-73 0.23453730680970608 2402 74-75 -0.014509170848199915 2402 76-77 0.17067135300623448 2402 78-79 0.1515789373294396 2402 80-81 0.27630251557483376 2402 82-83 0.4854694897114129 2402 84-85 0.2534296283798767 2402 86-87 0.5632961881064915 2402 88-89 0.5917395697216179 2402 90-91 0.334531266153121 2403 1 -0.02038447695800727 2403 2 0.1660813169494304 2403 3 0.1669351438896598 2403 4 0.11080780894620545 2403 5 0.09565561914286036 2403 6 -0.04770847845122006 2403 7 -0.04033537352930239 2403 8 -0.08415144260126084 2403 9 0.04614385169900714 2403 10-11 0.07989358973861016 2403 12-13 0.15796189693927687 2403 14-15 0.11237384770425507 2403 16-17 -0.013656316632797427 2403 18-19 0.09377779731997293 2403 20-21 0.10804314872446952 2403 22-23 0.01433358262021045 2403 24-25 0.08189087946236384 2403 26-27 0.15515752876904543 2403 28-29 0.053685869660291985 2403 30-31 0.1582803957488963 2403 32-33 0.10859814880566887 2403 34-35 0.11510273625837897 2403 36-37 0.10475715693478804 2403 38-39 0.07003894494852148 2403 40-41 0.11809749258806335 2403 42-43 0.12923863989878015 2403 44-45 0.1647993402143655 2403 46-47 0.1339303115290349 2403 48-49 0.007365466403989274 2403 50-51 0.1298839132657008 2403 52-53 0.17353594368734093 2403 54-55 0.15573515398157411 2403 56-57 0.19301858075905187 2403 58-59 0.12719291332552274 2403 60-61 0.18291832169371958 2403 62-63 0.27706023244476796 2403 64-65 0.10610516198826758 2403 66-67 0.19467028187327884 2403 68-69 -0.053822611423655076 2403 70-71 0.036324670735822906 2403 72-73 0.08293137924659533 2403 74-75 0.1752914347313137 2403 76-77 0.15889968881786842 2403 78-79 0.2512240140171329 2403 80-81 0.17434926690899744 2403 82-83 0.4787709251181056 2403 84-85 0.13374784982713095 2403 86-87 0.5394866642969731 2403 88-89 0.5917395697216179 2403 90-91 0.334531266153121 2404 1 -0.024827259616181152 2404 2 0.0132421947425172 2404 3 0.12255311919098943 2404 4 0.04693708299956967 2404 5 -0.0033962378372365265 2404 6 0.10874194607498566 2404 7 0.013390235316450116 2404 8 -0.0870686702308916 2404 9 0.16239783899514748 2404 10-11 0.12039918967941787 2404 12-13 0.10717201263747711 2404 14-15 0.06101408228035865 2404 16-17 -0.03393421955834697 2404 18-19 0.07518047118105642 2404 20-21 0.06339159756002033 2404 22-23 0.03381876064641176 2404 24-25 0.06796270298066531 2404 26-27 0.04095405654590678 2404 28-29 0.15579147911380176 2404 30-31 0.07537017933059786 2404 32-33 0.09092312049209283 2404 34-35 0.02582718195998268 2404 36-37 0.1667276407482987 2404 38-39 0.23431815180310878 2404 40-41 0.06505922489274951 2404 42-43 0.06679152143073708 2404 44-45 0.22190198550045892 2404 46-47 0.23137774349463314 2404 48-49 0.12644060323277984 2404 50-51 0.051717373878837236 2404 52-53 0.010671409361947326 2404 54-55 0.08121760821509127 2404 56-57 0.05457855540247181 2404 58-59 0.036459151876954365 2404 60-61 0.02362091869221672 2404 62-63 0.15452579001568978 2404 64-65 0.20064562018444576 2404 66-67 0.024116419569409686 2404 68-69 0.17915942768502902 2404 70-71 0.24641835206972473 2404 72-73 0.040356767767512736 2404 74-75 0.4269723098387814 2404 76-77 0.17641330565099622 2404 78-79 0.599321670786459 2404 80-81 0.20405567161540006 2404 82-83 0.42150657799373903 2404 84-85 0.4323443865115024 2404 86-87 0.07702616522320227 2404 88-89 0.03062845861050789 2404 90-91 0.334531266153121 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 3.0 31 247.0 32 1448.0 33 2760.0 34 3706.0 35 4616.0 36 6625.0 37 10119.0 38 26857.0 39 164596.0 40 167150.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.812803541108966 12.413204956109727 28.383493031919965 31.390498470861345 2 27.827270126864768 17.59433682686039 27.967200187604206 26.61119285867064 3 27.1566450070338 19.252253134291443 21.407996372312084 32.18310548636268 4 26.662149244963633 20.311392920358543 20.52627104014923 32.500186794528595 5 27.323015404751537 20.767429217756046 20.645304243198748 31.264251134293673 6 27.48249928502784 23.44000803860592 23.709765102659695 25.367727573706546 7 26.046629067289828 23.109188487273496 22.909511577396056 27.934670868040616 8 25.15439533966975 23.99678455763191 23.598718976005276 27.250101126693067 9 24.827698150347697 24.139263694615423 24.129988380092083 26.903049774944797 10-11 24.639873031250083 22.766774792786897 24.14003663749237 28.45331553847065 12-13 25.176037740224206 22.524843672303138 23.96573801874129 28.33338056873137 14-15 25.62795167561133 22.215408873899523 23.713372169418772 28.443267281070373 16-17 25.674070600602377 21.85199689792259 23.393373818363578 29.080558683111455 18-19 25.558644464311943 22.38558513064023 23.437946857600732 28.617823547447095 20-21 25.6784506102384 22.311382614453517 23.411537975971783 28.5986287993363 22-23 25.594328660464228 21.832415678373316 23.536497074411212 29.036758586751244 24-25 25.538934420949843 22.300690237989112 23.6137913621057 28.546583978955343 26-27 25.89294225859062 22.30867731438421 23.539588845918992 28.258791581106184 28-29 25.88662989176223 21.98210894887498 23.531344121898247 28.59991703746454 30-31 25.919976089961523 22.26738276544845 23.577925984517734 28.234715160072298 32-33 25.97019141168427 22.189210093528008 23.77599210172722 28.064606393060505 34-35 25.85517170541309 21.93953504296915 23.823056116684356 28.38223713493341 36-37 25.881132638875993 22.355887719077668 23.898914594891153 27.86406504715519 38-39 26.20020222446916 22.357338126802233 23.73860614912182 27.703853499606783 40-41 25.997356698810513 22.14420044876117 23.815291039461613 28.043151812966705 42-43 26.05074945283496 22.440213741838573 23.946487510235794 27.562549295090673 44-45 26.07733602883135 22.490561589867593 24.024102324152686 27.408000057148367 46-47 26.14549373723374 22.1983142314017 23.908012090977138 27.748179940387423 48-49 26.082144258368267 22.553740681851036 23.97732669791645 27.38678836186425 50-51 26.17914225871126 22.521029110337025 24.002867351396155 27.29696127955556 52-53 26.373859589208177 22.28911548031419 23.849343806410694 27.48768112406694 54-55 26.256631069465293 22.545636181999818 24.024104238007983 27.173628510526903 56-57 26.388823612351363 22.76548620576209 23.726911688677916 27.11877849320863 58-59 26.455423298461618 22.378939302435825 23.84816192943835 27.317475469664203 60-61 26.62133910098322 22.64693244555818 23.82477513748118 26.90695331597743 62-63 26.568923711273655 22.751461504633646 23.777439774712438 26.902175009380258 64-65 26.703852898396402 22.37880112918062 23.653887109125428 27.26345886329755 66-67 26.54257011215893 22.658053791136055 23.697550563873126 27.101825532831885 68-69 26.64111388322456 22.710620570380193 23.719682849446976 26.928582696948272 70-71 26.87296198387032 22.32323774506515 23.481300572992847 27.322499698071685 72-73 26.69787950080239 22.496611252200736 23.633205054297868 27.172304192699002 74-75 26.935888284924687 22.78866743534838 23.387141053093945 26.888303226632992 76-77 27.09910380231445 22.729052466718873 22.925911424345255 27.245932306621423 78-79 27.244773255890355 22.540375037779732 22.888605632137086 27.326246074192827 80-81 27.49760431385925 22.324546443827252 22.759830434147055 27.41801880816645 82-83 27.93825525575236 22.173481211719913 22.001738122827348 27.88652540970038 84-85 27.966738048224265 22.045776900186333 21.962343910782323 28.025141140807076 86-87 28.544612189472 21.53926032430705 21.319594216790478 28.596533269430463 88-89 27.956496101683808 20.480901526567514 20.520212278058047 31.042390093690624 90-91 25.10426692332371 22.794353545075392 22.361244786653835 29.740134744947067 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.5 8 2.0 9 3.0 10 5.5 11 8.0 12 8.5 13 9.0 14 15.0 15 27.5 16 43.5 17 60.0 18 82.0 19 110.0 20 151.0 21 219.0 22 298.0 23 408.0 24 543.5 25 663.5 26 828.5 27 1067.0 28 1374.0 29 1693.0 30 2043.0 31 2531.5 32 3061.5 33 3605.0 34 4288.0 35 5067.5 36 5989.0 37 6906.5 38 7857.5 39 9123.0 40 10563.5 41 12100.5 42 13640.5 43 15050.0 44 16416.0 45 17860.0 46 19354.0 47 21056.0 48 22612.5 49 23741.0 50 24749.0 51 25638.5 52 26289.0 53 26908.5 54 27526.0 55 27736.0 56 27696.5 57 27570.5 58 27175.5 59 26751.0 60 26388.0 61 25676.0 62 24708.0 63 23590.0 64 22045.5 65 20344.5 66 18605.0 67 16797.5 68 14848.5 69 12914.5 70 11173.5 71 9424.5 72 7704.5 73 6122.0 74 4790.5 75 3821.0 76 3059.5 77 2301.0 78 1709.5 79 1279.5 80 916.0 81 639.5 82 432.5 83 277.5 84 188.0 85 131.0 86 87.0 87 61.0 88 43.0 89 30.0 90 19.0 91 10.0 92 4.0 93 2.0 94 2.5 95 2.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.019581219549271243 3 2.5764762564830585E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.06242589731613553 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 4.034503070256836E-4 64-65 4.53120185598028E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.001314060446780552 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 5997.0 32-33 13434.0 34-35 14828.0 36-37 15663.0 38-39 17093.0 40-41 17938.0 42-43 18496.0 44-45 18806.0 46-47 18967.0 48-49 18986.0 50-51 18459.0 52-53 17935.0 54-55 17058.0 56-57 16497.0 58-59 15779.0 60-61 14707.0 62-63 13823.0 64-65 13119.0 66-67 12123.0 68-69 11159.0 70-71 10560.0 72-73 9759.0 74-75 8899.0 76-77 8125.0 78-79 7380.0 80-81 6810.0 82-83 6311.0 84-85 5608.0 86-87 5035.0 88-89 4573.0 90-91 4200.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.8762435385856 #Duplication Level Percentage of deduplicated Percentage of total 1 99.88001875791316 99.75641078103833 2 0.11605267636578256 0.23181810736027111 3 0.003928565721056233 0.011771111601406545 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0023188286308347527 0.0 0.0 0.0 0.0 2 0.0023188286308347527 0.0 0.0 0.0 0.0 3 0.0023188286308347527 0.0 0.0 0.0 0.0 4 0.0023188286308347527 0.0 0.0 0.0 2.5764762564830585E-4 5 0.002576476256483058 0.0 0.0 0.0 2.5764762564830585E-4 6 0.002834123882131364 0.0 0.0 0.0 2.5764762564830585E-4 7 0.003349419133427976 0.0 0.0 0.0 2.5764762564830585E-4 8 0.009532962148987316 0.0 0.0 0.0 2.5764762564830585E-4 9 0.009790609774635622 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 10-11 0.009790609774635622 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 12-13 0.009790609774635622 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 14-15 0.009919433587459774 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 16-17 0.010048257400283927 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 18-19 0.010048257400283927 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 20-21 0.01017708121310808 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 22-23 0.010821200277228845 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 24-25 0.010950024090052999 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 26-27 0.011207671715701303 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 28-29 0.011594143154173763 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 30-31 0.012238262218294528 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 32-33 0.01288238128241529 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 34-35 0.013526500346536057 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 36-37 0.01378414797218436 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 38-39 0.014299443223480975 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 40-41 0.014428267036305127 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 42-43 0.014428267036305127 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 44-45 0.014428267036305127 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 46-47 0.014685914661953433 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 48-49 0.01545885753889835 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 50-51 0.01545885753889835 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 52-53 0.015587681351722504 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 54-55 0.015845328977370808 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 56-57 0.015974152790194963 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 58-59 0.016231800415843267 0.0 2.5764762564830585E-4 0.0 2.5764762564830585E-4 60-61 0.016618271854315726 1.2882381282415292E-4 2.5764762564830585E-4 0.0 3.8647143847245875E-4 62-63 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