##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-23C_S9_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	862880
Sequences flagged as poor quality	0
Sequence length	61
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.455803819766366	40.0	40.0	40.0	40.0	40.0
2	39.185207676617836	40.0	40.0	40.0	40.0	40.0
3	39.14454385314296	40.0	40.0	40.0	40.0	40.0
4	38.98948173558316	40.0	40.0	40.0	40.0	40.0
5	39.06364268496199	40.0	40.0	40.0	40.0	40.0
6	39.16304468755794	40.0	40.0	40.0	40.0	40.0
7	39.2109493788244	40.0	40.0	40.0	40.0	40.0
8	39.19960597070276	40.0	40.0	40.0	40.0	40.0
9	39.2217967735954	40.0	40.0	40.0	40.0	40.0
10	39.22486556647506	40.0	40.0	40.0	40.0	40.0
11	39.21952994622659	40.0	40.0	40.0	40.0	40.0
12	39.228356202484704	40.0	40.0	40.0	40.0	40.0
13	39.065622102725754	40.0	40.0	40.0	40.0	40.0
14	39.22137956610421	40.0	40.0	40.0	40.0	40.0
15	39.14371407379937	40.0	40.0	40.0	40.0	40.0
16	39.307240867791585	40.0	40.0	40.0	40.0	40.0
17	39.26594196180233	40.0	40.0	40.0	40.0	40.0
18	39.33843408121639	40.0	40.0	40.0	40.0	40.0
19	39.28538846653069	40.0	40.0	40.0	40.0	40.0
20	39.28921286853328	40.0	40.0	40.0	40.0	40.0
21	39.283385870572964	40.0	40.0	40.0	40.0	40.0
22	39.332620990172444	40.0	40.0	40.0	40.0	40.0
23	39.329218431299836	40.0	40.0	40.0	40.0	40.0
24	39.32978397923234	40.0	40.0	40.0	40.0	40.0
25	39.28345076951604	40.0	40.0	40.0	40.0	40.0
26	39.24742258483219	40.0	40.0	40.0	40.0	40.0
27	39.272969590209534	40.0	40.0	40.0	40.0	40.0
28	39.26713332097163	40.0	40.0	40.0	40.0	40.0
29	39.27603838308919	40.0	40.0	40.0	40.0	40.0
30	39.26929816428704	40.0	40.0	40.0	40.0	40.0
31	39.222408677915816	40.0	40.0	40.0	40.0	40.0
32	39.10065826070833	40.0	40.0	40.0	40.0	40.0
33	39.235502039681066	40.0	40.0	40.0	40.0	40.0
34	39.24589282403115	40.0	40.0	40.0	40.0	40.0
35	39.25231318375672	40.0	40.0	40.0	40.0	40.0
36	39.21844984238828	40.0	40.0	40.0	40.0	40.0
37	39.23460040793621	40.0	40.0	40.0	40.0	40.0
38	39.22798071574263	40.0	40.0	40.0	40.0	40.0
39	37.99318792879659	40.0	40.0	40.0	40.0	40.0
40	39.22248748377526	40.0	40.0	40.0	40.0	40.0
41	39.23458186538105	40.0	40.0	40.0	40.0	40.0
42	39.20483033562025	40.0	40.0	40.0	40.0	40.0
43	39.22876413869831	40.0	40.0	40.0	40.0	40.0
44	39.17703968106805	40.0	40.0	40.0	40.0	40.0
45	39.07073521231226	40.0	40.0	40.0	40.0	40.0
46	39.04750602633043	40.0	40.0	40.0	40.0	40.0
47	39.133195809382535	40.0	40.0	40.0	40.0	40.0
48	39.201121824587425	40.0	40.0	40.0	40.0	40.0
49	39.17760986463935	40.0	40.0	40.0	40.0	40.0
50	39.13562024847024	40.0	40.0	40.0	40.0	40.0
51	39.09026052290005	40.0	40.0	40.0	40.0	40.0
52	39.085045429260155	40.0	40.0	40.0	40.0	40.0
53	38.98246801409234	40.0	40.0	40.0	40.0	40.0
54	38.854510476543666	40.0	40.0	40.0	40.0	40.0
55	38.87569071017986	40.0	40.0	40.0	40.0	40.0
56	38.88987112924161	40.0	40.0	40.0	40.0	40.0
57	38.77297886148711	40.0	40.0	40.0	40.0	40.0
58	38.76304932319674	40.0	40.0	40.0	40.0	40.0
59	38.71212683107732	40.0	40.0	40.0	40.0	40.0
60	38.63539773780827	40.0	40.0	40.0	40.0	40.0
61	38.49807157426293	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.04867711299709043
1101	2	0.020551072426357564
1101	3	-0.04693068633342534
1101	4	-0.048134051293992286
1101	5	-0.021987584369057345
1101	6	0.1490885377404041
1101	7	0.10132520975510317
1101	8	-0.00242897717885171
1101	9	0.027654766678438136
1101	10	-0.0644369681869037
1101	11	0.01737347086683627
1101	12	-0.052819195531121466
1101	13	0.040784877915918116
1101	14	-0.011775912681059708
1101	15	0.03437454372208748
1101	16	0.11350785941187524
1101	17	0.111416796127358
1101	18	0.06797642353615174
1101	19	0.046561422893347526
1101	20	0.0610175845968044
1101	21	-0.0945580140509108
1101	22	0.10761787816478119
1101	23	0.1148852621193015
1101	24	-0.016823805212645482
1101	25	0.20005393206017175
1101	26	0.10358050471059244
1101	27	0.06976769821425677
1101	28	0.029487171479161134
1101	29	0.03403239616896059
1101	30	0.0956943643004422
1101	31	0.016293189661567453
1101	32	0.0372168693049062
1101	33	-0.00426225430897631
1101	34	0.10018318248321378
1101	35	0.06565634872300308
1101	36	-0.105385936353386
1101	37	-0.12569612345729553
1101	38	-0.06692163330841794
1101	39	1.2779826813885933
1101	40	-0.04899454775372192
1101	41	-0.04874375108730078
1101	42	0.0384608955800374
1101	43	0.03609441124108059
1101	44	-0.10694378477300859
1101	45	-0.20175889213981435
1101	46	0.05484723599189323
1101	47	-0.08693035035732777
1101	48	-0.029190585894845356
1101	49	-0.004140186079439445
1101	50	0.030048449353323292
1101	51	0.03840802140263122
1101	52	-0.025501603818007368
1101	53	-0.0018511713300526367
1101	54	-0.03252225748570936
1101	55	-0.10854792114826495
1101	56	0.011154290153669422
1101	57	0.11644850802262852
1101	58	-0.021222085171082483
1101	59	0.06434685569302445
1101	60	-0.051265664814295064
1101	61	0.04610225907335774
1102	1	0.06862319730123545
1102	2	0.043383306982960335
1102	3	0.1398896518823065
1102	4	0.13449867433249807
1102	5	0.005917257479403304
1102	6	0.015587951411767165
1102	7	0.11312723502539512
1102	8	-0.15210783256474514
1102	9	-0.002938069737439264
1102	10	0.005092491290398016
1102	11	-0.030670895190866077
1102	12	-0.01936545261657585
1102	13	0.03899602539092939
1102	14	-0.0681137637315814
1102	15	0.14830464328792203
1102	16	0.010081373705403962
1102	17	0.06904708526754177
1102	18	-0.0380311657256982
1102	19	-0.05576471415979256
1102	20	-0.1307543225589214
1102	21	-0.026861421044728218
1102	22	0.09621826611628137
1102	23	-0.11665953307437604
1102	24	-0.04125468916987529
1102	25	0.009731341273550242
1102	26	-0.023333708964536015
1102	27	-0.12198848967499032
1102	28	-0.013300671868911706
1102	29	0.014125517103565244
1102	30	0.01454207919067585
1102	31	0.04601832256796712
1102	32	-0.05998164302371123
1102	33	0.013302454051469681
1102	34	0.0596148995615664
1102	35	-0.00924909988914635
1102	36	-0.019294657716812935
1102	37	-0.09458769510099785
1102	38	0.014079746843179919
1102	39	1.207899903731331
1102	40	-0.038751873717863816
1102	41	-0.020018363528791383
1102	42	-0.10183041386629554
1102	43	0.04828941817328314
1102	44	-0.06248341147187375
1102	45	0.08251461052145714
1102	46	0.09617633141098736
1102	47	0.07023401972450216
1102	48	-0.09806298003344693
1102	49	-0.006919352392422695
1102	50	-0.0028268245548943582
1102	51	0.07230190377850221
1102	52	0.02335702024315367
1102	53	-0.05852227082986161
1102	54	0.056272734984759154
1102	55	0.042916642909318625
1102	56	-0.044652249689953294
1102	57	-0.18692390340716258
1102	58	0.10462321838367217
1102	59	0.03851695701941793
1102	60	0.0964042683159505
1102	61	0.13487838842405608
1103	1	0.19839162552572276
1103	2	0.16665532619129664
1103	3	0.07379354353864898
1103	4	-0.015501140524342816
1103	5	0.06413155698496809
1103	6	0.07035329832508808
1103	7	-0.030201078283383254
1103	8	0.10008148530966565
1103	9	0.09821145769786455
1103	10	-0.019543186643019794
1103	11	0.04693688590008804
1103	12	-0.007855895791117007
1103	13	0.06891621810909498
1103	14	0.026298668578753848
1103	15	0.08094638756924866
1103	16	-0.0916563800002308
1103	17	0.02035972382736162
1103	18	-0.008135306108648876
1103	19	-0.0655275775098545
1103	20	-0.06855256835392254
1103	21	0.032975790508842806
1103	22	-0.05334990068607226
1103	23	-0.030056968259721373
1103	24	0.05612003371048502
1103	25	-0.05168963777406077
1103	26	-0.01281969336278621
1103	27	0.06950032053686783
1103	28	0.009532240660462321
1103	29	0.021564040317080924
1103	30	-0.0909641313044034
1103	31	-0.039388746409500186
1103	32	-0.08861641322666713
1103	33	-0.018867492558527488
1103	34	-0.02352284324707199
1103	35	-0.11261611340830058
1103	36	-0.09199138422703612
1103	37	0.02900753116774979
1103	38	-0.11015660374179248
1103	39	1.138942011104838
1103	40	0.006408246022154174
1103	41	-0.019583541591543963
1103	42	-0.00389814558221957
1103	43	0.04823228828371384
1103	44	0.028308678577531055
1103	45	0.10786018023076593
1103	46	-0.031573507382219645
1103	47	0.0288709217187062
1103	48	0.014386627965585319
1103	49	0.007232990805867701
1103	50	0.02660141633645452
1103	51	-0.05809072138752924
1103	52	-0.12103136990532448
1103	53	0.08099526725276007
1103	54	0.06665133158902847
1103	55	2.4484724811912884E-4
1103	56	-0.09486095893002755
1103	57	-0.09986324151507375
1103	58	-0.2095340443328908
1103	59	0.032233727128208045
1103	60	0.1439596115740116
1103	61	0.03694083033538931
1104	1	0.010079588107039683
1104	2	0.0025173966549019156
1104	3	-0.0180251650431984
1104	4	-0.3082579228780844
1104	5	-0.18212339771606878
1104	6	0.12484998650217705
1104	7	-0.20211175871541798
1104	8	-0.01642343232784782
1104	9	-0.09955082852161468
1104	10	-0.037538954069802344
1104	11	-0.16279868416952326
1104	12	-0.01841723579099863
1104	13	-0.023458579127627388
1104	14	-0.00914872483713225
1104	15	-0.016732974101799414
1104	16	-0.06659329054761542
1104	17	0.05169184397509241
1104	18	0.06187321442943272
1104	19	-6.053131646410748E-4
1104	20	-0.09972177190355325
1104	21	-0.12326665762801525
1104	22	-0.03534761659536656
1104	23	-0.1184761504944234
1104	24	-0.03610813858793449
1104	25	-0.11531834433031918
1104	26	-0.13855003489571516
1104	27	-0.034822780004937215
1104	28	-0.1011815349697116
1104	29	-0.006240392426327901
1104	30	-0.05581433220772425
1104	31	-0.05747140268860562
1104	32	-0.11961075296099466
1104	33	-0.19343914409295593
1104	34	-0.12414122034795128
1104	35	-0.03925042483857766
1104	36	-0.12830766616615819
1104	37	-0.04919460814440413
1104	38	-0.2380341860377584
1104	39	1.1501799980421907
1104	40	-0.21390061126453475
1104	41	-0.007841599310722813
1104	42	-0.1282719349082484
1104	43	-0.06243804292029864
1104	44	-0.11230789557966148
1104	45	-0.16007245701425177
1104	46	-0.11571341364000887
1104	47	-0.02042764086814941
1104	48	-0.046389952013157654
1104	49	-0.13495735768902506
1104	50	0.01432727983515747
1104	51	-0.06972948315621608
1104	52	-0.04723883984856769
1104	53	0.023723566354604486
1104	54	-0.0874156369077923
1104	55	-0.20887163974869338
1104	56	-0.15667911902599485
1104	57	-0.22890404832151034
1104	58	-0.07910736033189636
1104	59	-0.025358376412889072
1104	60	-0.034679524359795266
1104	61	-0.07942180680644384
1201	1	0.02019649855823502
1201	2	0.03426003394415034
1201	3	-0.03206642484402522
1201	4	0.07458213335555541
1201	5	-0.07643287677236543
1201	6	-0.11231642102451644
1201	7	0.0023145771953849703
1201	8	-0.10827957667873278
1201	9	0.01593287888978523
1201	10	-0.030940130088282558
1201	11	0.06650723338697873
1201	12	-0.00919646192988921
1201	13	-0.058921362392808874
1201	14	0.12594386611778674
1201	15	-0.010525405928156317
1201	16	0.024153402402866675
1201	17	-0.057510331835970874
1201	18	-0.04448297735451945
1201	19	0.153370462205217
1201	20	-0.06428364311393153
1201	21	0.0316277649400476
1201	22	0.015320546490777076
1201	23	0.07050147664093487
1201	24	0.06102620740118425
1201	25	-0.07656150874297651
1201	26	0.02616115668188712
1201	27	-0.04247531483340339
1201	28	0.03204160357718422
1201	29	0.020580461186646914
1201	30	0.025421092843480153
1201	31	0.08573679846190174
1201	32	-0.06551668970511315
1201	33	-0.03196412041662455
1201	34	-0.08312582041216388
1201	35	-0.10742648137664901
1201	36	0.11544577407112655
1201	37	-0.0369338302528206
1201	38	0.09563841431801734
1201	39	1.2559026769975006
1201	40	0.03082828865812104
1201	41	-0.024112481249552786
1201	42	0.18473485525589695
1201	43	0.12140716556277198
1201	44	0.022559589294012028
1201	45	0.0039108915673438105
1201	46	0.07259103911906806
1201	47	0.020950072665073094
1201	48	0.03905602869095759
1201	49	0.1365752449070854
1201	50	0.011275484533761926
1201	51	-0.004841047337983184
1201	52	-0.03520316803822965
1201	53	-0.021361965916767645
1201	54	0.012277414871427084
1201	55	0.16565664241856837
1201	56	0.009337687822799978
1201	57	0.07405443514954158
1201	58	0.11820240761053213
1201	59	0.058575105812039396
1201	60	0.0539104379456532
1201	61	0.10636574557761946
1202	1	0.05048452471370268
1202	2	0.017197464418572395
1202	3	-0.1295427040421302
1202	4	0.07156224689674673
1202	5	-0.08565292145338788
1202	6	-0.06309607034521036
1202	7	0.07975113465143835
1202	8	0.06479779788769235
1202	9	-0.05355036124156953
1202	10	0.010076828811136807
1202	11	-0.03182584099091201
1202	12	-0.051061774677272354
1202	13	0.08913010124179976
1202	14	0.01179015315841525
1202	15	0.07104845928645886
1202	16	-0.044892998806780327
1202	17	-0.012042680734154487
1202	18	-0.04385198780093447
1202	19	0.027884788859957155
1202	20	0.05106557346452689
1202	21	-0.014237156340577428
1202	22	-0.0347813957805414
1202	23	0.0074130729469032985
1202	24	-0.09660880576597464
1202	25	-0.10823655123321174
1202	26	0.1314625504776643
1202	27	0.04672054543195969
1202	28	-0.08666251481671594
1202	29	-0.03992542255352305
1202	30	-0.030672084687488166
1202	31	0.02384231563724626
1202	32	0.07681893712049259
1202	33	-0.11020629747830668
1202	34	-0.08583232242713024
1202	35	-0.12035174314367225
1202	36	-0.2782104595490651
1202	37	-0.27524845504485285
1202	38	-0.2703286681809658
1202	39	0.9464840763645626
1202	40	-0.2102113035622395
1202	41	-0.1750466597008895
1202	42	-0.1722548708519014
1202	43	-0.1018723920903355
1202	44	-0.13716676383169357
1202	45	-0.17079354970749705
1202	46	-0.09033510634987607
1202	47	-0.1054994952574475
1202	48	-0.09873499611573777
1202	49	-0.15953876161015046
1202	50	-0.13698595722988216
1202	51	-0.16935047010652227
1202	52	-0.007704474466223132
1202	53	-0.10770096294410081
1202	54	-0.2008904824271056
1202	55	-0.14593401840846099
1202	56	-0.19943174801397134
1202	57	-0.07230823453771507
1202	58	0.04325709820802359
1202	59	-0.12146320200692884
1202	60	-0.12932766221655356
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2403	2	0.04446281627927107
2403	3	0.12781046846033917
2403	4	0.17782082443469704
2403	5	0.029016869637729314
2403	6	0.031436926848279256
2403	7	0.13975620916272646
2403	8	0.08065108322517744
2403	9	0.10737511194707139
2403	10	0.052163948336847454
2403	11	0.07958460484834973
2403	12	0.13886985561229892
2403	13	0.05374689324363402
2403	14	-0.01937600794111205
2403	15	0.058755775748430494
2403	16	0.01660964215901828
2403	17	0.10581509103786146
2403	18	0.10056920262820768
2403	19	0.050057924484377736
2403	20	0.08444403626349839
2403	21	0.003623598034693032
2403	22	-0.026383801420301722
2403	23	0.1257279048336244
2403	24	0.037212294422388936
2403	25	0.0730496153848108
2403	26	0.005536224046586824
2403	27	0.028756829958140884
2403	28	0.08106056994682831
2403	29	0.04109842478948167
2403	30	0.029938226757572295
2403	31	0.06049156381012466
2403	32	0.036210317179182994
2403	33	0.05769710539961892
2403	34	-0.01570600849846926
2403	35	0.11990226025213957
2403	36	0.013777410108176014
2403	37	-0.007316983010305478
2403	38	0.0797564994420199
2403	39	1.2934466354327014
2403	40	0.018858073832269895
2403	41	0.0796409643614453
2403	42	0.1488459303788403
2403	43	0.06598851890564816
2403	44	0.04183519174821981
2403	45	0.06386187585891179
2403	46	0.15787305048439038
2403	47	0.07427766478400599
2403	48	0.13579404334029022
2403	49	-0.016988303521785042
2403	50	0.1201547558628846
2403	51	0.0012920248533561107
2403	52	0.13954079180874857
2403	53	0.09756587858392862
2403	54	0.038989265547002105
2403	55	0.025344765245080225
2403	56	0.11874637441412972
2403	57	0.2402620490097931
2403	58	0.06245362302580304
2403	59	0.06845480908971524
2403	60	0.11931358495287014
2403	61	0.16987928734128843
2404	1	-0.00433241148905239
2404	2	-0.05813344935813092
2404	3	-0.07322359638564535
2404	4	0.09672622002797482
2404	5	0.11593377794926596
2404	6	-0.10236446410744549
2404	7	-0.04133604208631425
2404	8	6.149181930226177E-5
2404	9	-0.11604354143519657
2404	10	0.05137148373954403
2404	11	-0.05953633153367832
2404	12	0.09786403993105353
2404	13	0.06261674770153292
2404	14	-0.023542365198075288
2404	15	0.09522887429486815
2404	16	-0.07866365315085488
2404	17	-0.09765385791932601
2404	18	0.06605077493771461
2404	19	0.02252231606559718
2404	20	0.0013958882619959923
2404	21	-0.04374335238709648
2404	22	-0.14136218548296853
2404	23	0.0400397111893227
2404	24	-0.08195893753455863
2404	25	-0.05826785559913361
2404	26	-0.030008011301873694
2404	27	0.011601605955142702
2404	28	0.044500108660287196
2404	29	0.0450274935717232
2404	30	-0.11216995170534005
2404	31	-0.06668829507650287
2404	32	0.013576040295383507
2404	33	-0.14771129638781844
2404	34	-0.05433217586547556
2404	35	-0.009767718542669002
2404	36	0.03974387098862309
2404	37	-0.1377918262752047
2404	38	-0.018709199480333893
2404	39	1.214512427044106
2404	40	-0.05282502712791626
2404	41	0.07613743666809114
2404	42	-0.044171616709327566
2404	43	0.0020852108591284946
2404	44	-0.09469304395983613
2404	45	-0.012921518231451046
2404	46	0.09499385920409509
2404	47	0.02493969821941988
2404	48	-0.01821575847733925
2404	49	0.05696341052813381
2404	50	0.14941620112160336
2404	51	0.1199497893507413
2404	52	-0.024038330018171905
2404	53	-0.0563193608752286
2404	54	0.11457099923804037
2404	55	0.02561418066294152
2404	56	-0.06412131316600522
2404	57	0.01377555437351674
2404	58	0.009621710487742519
2404	59	-0.07036965760655534
2404	60	0.10827938906277979
2404	61	0.05615836310549582
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	3.0
17	23.0
18	71.0
19	112.0
20	164.0
21	265.0
22	370.0
23	489.0
24	749.0
25	962.0
26	1354.0
27	1660.0
28	2331.0
29	2573.0
30	3341.0
31	4236.0
32	5500.0
33	6537.0
34	8225.0
35	10703.0
36	15298.0
37	22479.0
38	58574.0
39	394728.0
40	322133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.93498516595587	11.308988503615797	32.37843037270536	31.377595957722974
2	28.317049306518406	16.48786697024359	28.766038645774888	26.429045077463126
3	27.80212775820508	19.644562395698127	21.10606341553866	31.447246430558128
4	26.762585759317638	19.608520304097905	20.81564064528092	32.81325329130354
5	27.48250046356388	19.938925458928242	20.24928147598739	32.329292601520486
6	29.732871314667157	22.415631374003336	22.849295382903765	25.002201928425738
7	27.216530687928795	21.82609401075468	22.82739198961617	28.12998331170035
8	25.369692193584275	23.902744298164286	23.156638234748748	27.570925273502688
9	25.78713146671611	23.957908399777487	23.543134618950493	26.711825514555905
10	26.062836083812353	21.989847951047654	23.610235490450584	28.337080474689415
11	25.26318839236047	22.747079547561654	23.341716113480437	28.648015946597443
12	27.335782495827925	22.34285184498424	22.953133691822732	27.3682319673651
13	25.29436306323011	21.032472649731133	23.303819766363805	30.36934452067495
14	25.948335805674024	22.24782124976822	23.10529853513814	28.69854440941962
15	26.41143612089746	21.58608381234934	23.008761357315038	28.99371870943816
16	25.820276284071948	22.29533654737623	22.948845725941034	28.93554144261079
17	25.980553495271646	21.192518078991284	22.916743927313185	29.91018449842388
18	27.762956610420915	21.86665585017615	22.783932875950306	27.586454663452624
19	25.75305952160208	21.321852401260895	23.967411459299093	28.95767661783794
20	26.138976450954942	21.603815130725014	22.807806415724087	29.44940200259596
21	26.415028740960505	22.160555349527165	23.714073799369555	27.710342110142776
22	27.05845540515483	21.49742722047098	22.944789541998887	28.4993278323753
23	26.079872056369368	22.79447895419989	22.77512516224736	28.350523827183387
24	26.70324958279251	23.532472649731133	22.67545892824031	27.088818839236044
25	25.90163174485444	21.801641016132024	22.917207491192286	29.37951974782125
26	26.326835712961245	22.15348600037085	23.80203504542926	27.717643241238644
27	26.749026515853885	22.789959206378636	22.455497867606155	28.00551641016132
28	26.224040422770255	22.566521416651213	22.565362506953456	28.644075653625066
29	26.556763396996107	23.87875486742073	22.322339143333952	27.242142592249213
30	28.0974179491934	23.65554885963286	22.06529297237159	26.18174021880215
31	26.526747635824215	23.153509178564807	23.274035787131467	27.045707398479514
32	26.87129148896718	23.557505099202668	21.855762099017245	27.715441312812906
33	27.076691293640447	25.426626104592202	21.703259452411995	25.793423149355355
34	26.880910439458557	24.070554422399407	21.600338401631745	27.44819673651029
35	28.22118950491378	24.372450398664935	21.176409234192473	26.22995086222881
36	27.72135175227146	26.309567958464676	20.781336918227332	25.187743371036532
37	27.111185796402744	24.903578713146672	21.879635638791026	26.105599851659562
38	27.392337289078434	25.355089931392545	20.429144261079177	26.823428518449845
39	28.254272060098927	26.82716174913721	19.869490582627055	25.0490756081368
40	27.382254774707953	26.773131837567217	20.08286204338958	25.76175134433525
41	28.638628778045614	25.77751251622474	19.86035138142036	25.723507324309292
42	28.862530131652143	26.622589467828668	19.39145651770814	25.123423882811053
43	27.499652327090672	27.376112553309845	19.511519562395698	25.61271555720378
44	27.550296680882624	26.559428889300946	20.327739662525495	25.562534767290934
45	27.63675134433525	27.100523827183387	19.084924902651583	26.17779992582978
46	27.2264973113295	26.82481921008715	20.18507787873169	25.763605599851658
47	28.46004079362136	27.00375486742073	18.813044687557944	25.723159651399964
48	28.463401631744855	27.560958650101984	18.653578713146672	25.32206100500649
49	28.076209901724457	27.40647598739106	18.626923790098278	25.890390320786207
50	28.097070276284068	27.558177266827364	18.414263860559984	25.930488596328573
51	28.036806972000743	27.87247357685889	18.44497496755053	25.645744483589837
52	27.522946412015575	27.86679491933988	18.380307806415725	26.22995086222881
53	27.665492304839606	27.881976636380497	18.194534581865383	26.25799647691452
54	27.693537919525312	28.190941961802334	18.04561468570369	26.069905432968664
55	27.23217596884851	28.276237715557208	18.009804376043018	26.48178193955127
56	27.023572223252362	28.253175412571853	17.942703504542926	26.78054885963286
57	26.889138698312625	28.5488132764695	17.867026701279435	26.69502132393844
58	26.459530873354346	28.539657889857224	17.955915075097348	27.044896161691078
59	26.62676154274059	28.467341924717225	17.665724086779157	27.24017244576303
60	26.41641943259781	28.975871500092715	17.7140506211756	26.893658446133877
61	26.389648618579642	28.711060634155388	17.60407009085852	27.295220656406453
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.5
2	3.0
3	4.0
4	3.0
5	2.0
6	3.5
7	3.0
8	1.0
9	8.5
10	16.0
11	14.5
12	12.0
13	11.0
14	15.0
15	19.0
16	26.0
17	52.0
18	71.0
19	89.0
20	132.0
21	157.0
22	182.5
23	208.0
24	307.5
25	539.0
26	671.0
27	841.5
28	1012.0
29	1310.0
30	2017.0
31	2426.0
32	3030.5
33	3635.0
34	4454.5
35	6315.5
36	7357.0
37	8782.5
38	10208.0
39	11780.5
40	15476.0
41	17599.0
42	19829.0
43	24994.0
44	27929.0
45	31552.0
46	35175.0
47	38996.0
48	46759.5
49	50702.0
50	54674.0
51	58646.0
52	61903.5
53	67167.5
54	69174.0
55	70051.0
56	70928.0
57	69762.0
58	65916.5
59	63237.0
60	58753.0
61	53537.5
62	52806.0
63	43788.5
64	34771.0
65	30312.0
66	22393.0
67	18933.0
68	16154.5
69	13376.0
70	11248.5
71	7507.5
72	5894.0
73	4763.0
74	3632.0
75	2983.0
76	1897.5
77	1461.0
78	1113.0
79	765.0
80	614.5
81	361.5
82	259.0
83	196.0
84	106.0
85	79.0
86	58.5
87	38.0
88	29.0
89	18.0
90	16.0
91	11.0
92	6.0
93	4.0
94	3.0
95	4.0
96	6.5
97	9.0
98	7.5
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03198590765807528
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	5.794548488781754E-4
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.1203643612089746
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	862880.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.91980325656685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.27942634483713	72.40552483948183
2	11.086484955068185	18.60751272472421
3	1.8867096447358271	4.749969065654933
4	0.41907020471675843	1.4067315652207824
5	0.1543456516268227	0.6476328359014785
6	0.06334995358965313	0.3189789384933799
7	0.035299744097622536	0.2073643305775748
8	0.023157108493900017	0.155467199103925
9	0.01675365279166448	0.12653669214947838
>10	0.035102610198698266	0.3695788632518554
>50	1.5006492187527609E-4	0.010074734975588195
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	1.5006492187527609E-4	0.9946282104649447
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAAGGATCTGGGGGGGGGGGGGGG	7898	0.9153068792879658	TruSeq Adapter, Index 9 (97% over 38bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0544919339885037	1.1589096977563508E-4	0.0	0.0	1.1589096977563508E-4
2	1.0570415353235676	1.1589096977563508E-4	0.0	0.0	1.1589096977563508E-4
3	1.058779899870202	1.1589096977563508E-4	0.0	0.0	1.1589096977563508E-4
4	1.0599388095679585	1.1589096977563508E-4	0.0	0.0	1.1589096977563508E-4
5	1.0609818282959391	1.1589096977563508E-4	0.0	0.0	1.1589096977563508E-4
6	1.066776376784721	1.1589096977563508E-4	0.0	0.0	1.1589096977563508E-4
7	1.0721073613944	1.1589096977563508E-4	0.0	0.0	1.1589096977563508E-4
8	1.1126692008158725	1.1589096977563508E-4	0.0	0.0	1.1589096977563508E-4
9	1.1170730576673467	1.1589096977563508E-4	0.0	0.0	1.1589096977563508E-4
10	1.1254172074911923	1.1589096977563508E-4	0.0	0.0	1.1589096977563508E-4
11	1.136310958650102	1.1589096977563508E-4	0.0	0.0	1.1589096977563508E-4
12	1.1545058409048767	1.1589096977563508E-4	0.0	0.0	1.1589096977563508E-4
13	1.1854487298349712	1.1589096977563508E-4	0.0	0.0	3.4767290932690527E-4
14	1.2309938809567957	1.1589096977563508E-4	0.0	0.0	3.4767290932690527E-4
15	1.299601335063972	2.3178193955127017E-4	0.0	0.0	3.4767290932690527E-4
16	1.387214908214352	2.3178193955127017E-4	0.0	0.0	3.4767290932690527E-4
17	1.498354348229186	2.3178193955127017E-4	0.0	0.0	3.4767290932690527E-4
18	1.6645419988874466	2.3178193955127017E-4	0.0	0.0	3.4767290932690527E-4
19	1.894353791952531	2.3178193955127017E-4	0.0	0.0	3.4767290932690527E-4
20	2.1838494344520676	2.3178193955127017E-4	0.0	0.0	3.4767290932690527E-4
21	2.5690710179862783	2.3178193955127017E-4	0.0	0.0	3.4767290932690527E-4
22	3.0229000556276655	2.3178193955127017E-4	0.0	0.0	3.4767290932690527E-4
23	3.5719914704246247	2.3178193955127017E-4	0.0	0.0	3.4767290932690527E-4
24	4.216808826256258	2.3178193955127017E-4	0.0	0.0	3.4767290932690527E-4
25	4.959322269608752	2.3178193955127017E-4	0.0	0.0	3.4767290932690527E-4
26	5.826534396439829	2.3178193955127017E-4	0.0	0.0	3.4767290932690527E-4
27	6.786343408121639	2.3178193955127017E-4	0.0	0.0	3.4767290932690527E-4
28	7.812210272575561	2.3178193955127017E-4	0.0	0.0	3.4767290932690527E-4
29	8.92522714630076	2.3178193955127017E-4	0.0	0.0	3.4767290932690527E-4
30	10.17522714630076	2.3178193955127017E-4	0.0	0.0	3.4767290932690527E-4
31	11.470308733543483	2.3178193955127017E-4	0.0	0.0	3.4767290932690527E-4
32	12.86691081030966	2.3178193955127017E-4	0.0	0.0	3.4767290932690527E-4
33	14.348113295012052	2.3178193955127017E-4	0.0	0.0	3.4767290932690527E-4
34	15.89154923048396	2.3178193955127017E-4	0.0	0.0	3.4767290932690527E-4
35	17.480762099017245	2.3178193955127017E-4	0.0	0.0	3.4767290932690527E-4
36	19.145999443723344	2.3178193955127017E-4	0.0	0.0	3.4767290932690527E-4
37	20.84032542184313	2.3178193955127017E-4	0.0	0.0	4.6356387910254034E-4
38	22.638026145002783	2.3178193955127017E-4	0.0	0.0	4.6356387910254034E-4
39	24.42738271833859	2.3178193955127017E-4	0.0	0.0	4.6356387910254034E-4
40	26.32115705544224	2.3178193955127017E-4	0.0	0.0	4.6356387910254034E-4
41	28.2710226219173	2.3178193955127017E-4	0.0	0.0	4.6356387910254034E-4
42	30.290075097348414	2.3178193955127017E-4	0.0	0.0	4.6356387910254034E-4
43	32.285138142035976	2.3178193955127017E-4	0.0	0.0	4.6356387910254034E-4
44	34.29642592249212	2.3178193955127017E-4	0.0	0.0	4.6356387910254034E-4
45	36.335063971815316	2.3178193955127017E-4	0.0	0.0	4.6356387910254034E-4
46	38.27681717040608	2.3178193955127017E-4	0.0	0.0	5.794548488781754E-4
47	40.27025774151678	2.3178193955127017E-4	0.0	0.0	5.794548488781754E-4
48	42.297190802892636	2.3178193955127017E-4	0.0	0.0	5.794548488781754E-4
49	44.253314481735586	2.3178193955127017E-4	0.0	0.0	5.794548488781754E-4
50	46.196342480993884	2.3178193955127017E-4	0.0	0.0	5.794548488781754E-4
>>END_MODULE