##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-23C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 547346 Sequences flagged as poor quality 0 Sequence length 31-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.56512334062914 40.0 40.0 40.0 40.0 40.0 2 39.35720367007341 40.0 40.0 40.0 40.0 40.0 3 39.32659780102531 40.0 40.0 40.0 40.0 40.0 4 39.16984138004114 40.0 40.0 40.0 40.0 40.0 5 39.24944002513949 40.0 40.0 40.0 40.0 40.0 6 39.35682365450739 40.0 40.0 40.0 40.0 40.0 7 39.404837159675964 40.0 40.0 40.0 40.0 40.0 8 39.39513945475074 40.0 40.0 40.0 40.0 40.0 9 39.408966174960625 40.0 40.0 40.0 40.0 40.0 10-11 39.4089625209648 40.0 40.0 40.0 40.0 40.0 12-13 39.34272653860629 40.0 40.0 40.0 40.0 40.0 14-15 39.3729560460842 40.0 40.0 40.0 40.0 40.0 16-17 39.47322534557665 40.0 40.0 40.0 40.0 40.0 18-19 39.50032703262653 40.0 40.0 40.0 40.0 40.0 20-21 39.47898038900439 40.0 40.0 40.0 40.0 40.0 22-23 39.52145809049486 40.0 40.0 40.0 40.0 40.0 24-25 39.49645379704977 40.0 40.0 40.0 40.0 40.0 26-27 39.468990364413 40.0 40.0 40.0 40.0 40.0 28-29 39.47949560241602 40.0 40.0 40.0 40.0 40.0 30-31 39.45486036254947 40.0 40.0 40.0 40.0 40.0 32-33 39.457646516810236 40.0 40.0 40.0 40.0 40.0 34-35 39.45369240333797 40.0 40.0 40.0 40.0 40.0 36-37 39.436533419203926 40.0 40.0 40.0 40.0 40.0 38-39 39.38018018660212 40.0 40.0 40.0 40.0 40.0 40-41 39.422183412539084 40.0 40.0 40.0 40.0 40.0 42-43 39.403315338837515 40.0 40.0 40.0 40.0 40.0 44-45 39.29741418097046 40.0 40.0 40.0 40.0 40.0 46-47 39.287091280623 40.0 40.0 40.0 40.0 40.0 48-49 39.370511542182825 40.0 40.0 40.0 40.0 40.0 50-51 39.32217633514718 40.0 40.0 40.0 40.0 40.0 52-53 39.278803865227914 40.0 40.0 40.0 40.0 40.0 54-55 39.18408499063627 40.0 40.0 40.0 40.0 40.0 56-57 39.20304236172389 40.0 40.0 40.0 40.0 40.0 58-59 39.14864031948906 40.0 40.0 40.0 40.0 40.0 60-61 39.071458799563004 40.0 40.0 40.0 40.0 40.0 62-63 39.384474823100376 40.0 40.0 40.0 40.0 40.0 64-65 39.391810303763336 40.0 40.0 40.0 40.0 40.0 66-67 39.384066127539214 40.0 40.0 40.0 40.0 40.0 68-69 39.381810108573816 40.0 40.0 40.0 40.0 40.0 70-71 39.39015418474017 40.0 40.0 40.0 40.0 40.0 72-73 39.37741743876437 40.0 40.0 40.0 40.0 40.0 74-75 39.383833412150196 40.0 40.0 40.0 40.0 40.0 76-77 39.387604485896944 40.0 40.0 40.0 40.0 40.0 78-79 39.40141759961482 40.0 40.0 40.0 40.0 40.0 80-81 39.384635890904164 40.0 40.0 40.0 40.0 40.0 82-83 39.378742137401446 40.0 40.0 40.0 40.0 40.0 84-85 39.345114841470306 40.0 40.0 40.0 40.0 40.0 86-87 39.321579535474655 40.0 40.0 40.0 40.0 40.0 88-89 39.3798314076165 40.0 40.0 40.0 40.0 40.0 90-91 39.421465914685164 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.03410000695595272 1101 2 -0.03953932564852636 1101 3 0.041351141572640415 1101 4 0.06039666751226491 1101 5 -0.11650835053748665 1101 6 0.04397691880401311 1101 7 0.08483619439112289 1101 8 -0.050201773232807057 1101 9 0.08622027632157625 1101 10-11 0.039728202681402536 1101 12-13 0.024101332740698922 1101 14-15 -0.00243926503907943 1101 16-17 0.04143582495292719 1101 18-19 0.04493099471923756 1101 20-21 0.12701887207175133 1101 22-23 -0.021015776098117556 1101 24-25 -0.012385113643787804 1101 26-27 0.008039909724097072 1101 28-29 0.08398052769454978 1101 30-31 0.06197612449042822 1101 32-33 0.036436137355977394 1101 34-35 -0.014210823495808711 1101 36-37 -0.1401427001879867 1101 38-39 -0.15926872295496253 1101 40-41 0.012002128631237952 1101 42-43 -0.08806178690539213 1101 44-45 -0.15435909042150087 1101 46-47 -0.0016613256024626821 1101 48-49 0.04973181736941257 1101 50-51 0.03293903279180199 1101 52-53 -0.010248448863578119 1101 54-55 -0.12202006957461009 1101 56-57 -0.15903471597346197 1101 58-59 0.11511650816472496 1101 60-61 0.30139340698281103 1101 62-63 0.04543428594899268 1101 64-65 0.07071757976108728 1101 66-67 -0.13724045999114054 1101 68-69 -0.12411118258723519 1101 70-71 0.06807894251902269 1101 72-73 0.09759256588417031 1101 74-75 0.06143845447334684 1101 76-77 0.1924978276930176 1101 78-79 -9.233612522763224E-5 1101 80-81 -0.15318559017647004 1101 82-83 0.08952406155040649 1101 84-85 0.18503502818529682 1101 86-87 -0.03473388581213044 1101 88-89 0.47173611408074123 1101 90-91 0.22901374564977317 1102 1 0.09072432558971855 1102 2 0.1581806460806945 1102 3 0.030055949736400578 1102 4 -0.08725418512057814 1102 5 -0.10519348066668499 1102 6 -0.0887711023236406 1102 7 -0.020795495115727647 1102 8 -0.1537902268180673 1102 9 -0.02997195839801492 1102 10-11 -0.006400389994752231 1102 12-13 -0.1169622978238749 1102 14-15 -0.07453958365925217 1102 16-17 -0.03716152474378731 1102 18-19 -0.01167615135901201 1102 20-21 0.015165643972174792 1102 22-23 0.033070941720502844 1102 24-25 -0.08952628878321889 1102 26-27 -0.0441167208716422 1102 28-29 -0.037687120140780905 1102 30-31 0.060459275993260064 1102 32-33 -0.001949981923168309 1102 34-35 0.037133319150719046 1102 36-37 -0.12746575786322722 1102 38-39 -0.01733375941620352 1102 40-41 0.010104413579576033 1102 42-43 -0.007104131140003744 1102 44-45 -0.21428012865356294 1102 46-47 0.07498660442305294 1102 48-49 -0.0668173122794613 1102 50-51 -0.04197240506726985 1102 52-53 -0.1280869547895307 1102 54-55 0.02619768909838882 1102 56-57 0.03540684590254983 1102 58-59 -0.17034115268128147 1102 60-61 -0.11219473769676824 1102 62-63 -0.11405418535589007 1102 64-65 -0.21026896265497896 1102 66-67 -0.0892196635832434 1102 68-69 -0.4817234735711793 1102 70-71 -0.3091561673964378 1102 72-73 -0.12930054561352478 1102 74-75 -0.36231218234579643 1102 76-77 -0.15747717182460974 1102 78-79 0.11018264859227855 1102 80-81 -0.22676760346210756 1102 82-83 0.1033620753884179 1102 84-85 0.1463985933359382 1102 86-87 -0.42336883552164295 1102 88-89 -0.37390491156028816 1102 90-91 -0.1945156661149383 1103 1 0.058608596946214675 1103 2 0.05559910739383156 1103 3 0.0018979527123406115 1103 4 -0.0934941710858368 1103 5 0.075796692602772 1103 6 -0.0033748744812598375 1103 7 0.1478756550487219 1103 8 0.061577006093394004 1103 9 0.13620770394744142 1103 10-11 0.013063151474916879 1103 12-13 0.03577330912621335 1103 14-15 -0.022916052619066818 1103 16-17 -0.13463052398812891 1103 18-19 -0.039673199557554994 1103 20-21 -0.04960016341554052 1103 22-23 -0.03992578860410134 1103 24-25 -0.006322988688495457 1103 26-27 -0.0013582511408856135 1103 28-29 0.04486675144298147 1103 30-31 -0.036138185360911734 1103 32-33 -0.11296466247768677 1103 34-35 -0.02569557608663331 1103 36-37 -0.05105272760670232 1103 38-39 -0.06546852499927525 1103 40-41 0.0475282143981417 1103 42-43 -0.006413101974168001 1103 44-45 -0.0624557076825667 1103 46-47 -0.11280814093407088 1103 48-49 -0.11524423994249844 1103 50-51 -0.030890990347096192 1103 52-53 -0.027167565677480354 1103 54-55 0.12011339709076907 1103 56-57 -0.07380475573230427 1103 58-59 0.07274068538072243 1103 60-61 -0.00794777958135029 1103 62-63 0.12917238625802696 1103 64-65 0.01742975516275891 1103 66-67 0.10895374675208558 1103 68-69 0.10423078837655453 1103 70-71 -0.04174650851086881 1103 72-73 -0.08534555042921 1103 74-75 -0.05880920384009869 1103 76-77 -0.11613551579920056 1103 78-79 -0.1283308319193921 1103 80-81 -0.0999869407591163 1103 82-83 0.08344978503681233 1103 84-85 0.4416731564895713 1103 86-87 0.677784669358573 1103 88-89 0.7794284217730478 1103 90-91 0.05758517422120235 1104 1 0.07770521942939013 1104 2 0.11109100118174808 1104 3 0.012149427823409553 1104 4 0.04125819054316082 1104 5 -0.0011343405125643358 1104 6 0.11451954219320726 1104 7 -0.16389902835378933 1104 8 0.005655855292133083 1104 9 -0.055121549672726644 1104 10-11 0.044123371328090855 1104 12-13 0.0026444792849176224 1104 14-15 0.020755329469956507 1104 16-17 0.040914336027064735 1104 18-19 0.06712676299488152 1104 20-21 -0.02230023499925693 1104 22-23 -0.020121914089422432 1104 24-25 -0.06344687675952798 1104 26-27 -0.06235526392088531 1104 28-29 -0.010779674869738187 1104 30-31 0.010282076878326052 1104 32-33 -0.07709281896863729 1104 34-35 -0.1614928234304145 1104 36-37 -0.06672968293480608 1104 38-39 -0.002844837115183907 1104 40-41 -0.07118125082581628 1104 42-43 -0.09104766004262643 1104 44-45 -0.18008869186370902 1104 46-47 -0.014344122398618708 1104 48-49 -0.07748861666451035 1104 50-51 -0.0348145136784197 1104 52-53 -0.05828858599288367 1104 54-55 0.00245638491454514 1104 56-57 -0.0290822875780421 1104 58-59 0.10889236388918988 1104 60-61 0.022079471536713413 1104 62-63 0.15243604267986655 1104 64-65 0.20220506928928472 1104 66-67 -0.07015714979181809 1104 68-69 0.09442123175517025 1104 70-71 0.07933665726161365 1104 72-73 0.14302793778778522 1104 74-75 0.22392247052286507 1104 76-77 0.2909699085505508 1104 78-79 0.3860598837479188 1104 80-81 -8.297998559214648E-5 1104 82-83 0.06512090589978925 1104 84-85 0.5927029047733186 1104 86-87 0.4136337259623417 1104 88-89 0.5370041793488056 1104 90-91 0.6290137456497717 1201 1 0.13934803743242696 1201 2 0.1496301433966778 1201 3 0.1070419211257061 1201 4 -0.07675395398341323 1201 5 0.04413685050412397 1201 6 0.07421990691241831 1201 7 -0.008902151551566817 1201 8 0.004411368814544403 1201 9 -0.07299806274622966 1201 10-11 0.02034233704640087 1201 12-13 -0.06466083206834128 1201 14-15 0.04666748693663436 1201 16-17 0.1025946963051041 1201 18-19 -0.010833365511530246 1201 20-21 -0.009155646119118899 1201 22-23 0.03428323255709387 1201 24-25 -0.020318790504958884 1201 26-27 -0.050090122801869086 1201 28-29 0.012236934791246767 1201 30-31 0.006142042595044472 1201 32-33 0.08342531253332197 1201 34-35 0.05064035068036077 1201 36-37 -0.04536427250600639 1201 38-39 0.08339346884969956 1201 40-41 0.0013854162439841389 1201 42-43 0.06453483152854034 1201 44-45 0.11977821764860863 1201 46-47 0.08323354977024167 1201 48-49 0.006532181220649136 1201 50-51 0.02365845275416234 1201 52-53 0.12535830797087044 1201 54-55 0.01849035126419807 1201 56-57 0.019786860867696987 1201 58-59 0.0701498385889252 1201 60-61 0.19924015047165256 1201 62-63 -0.0936817414643869 1201 64-65 -0.195240301145283 1201 66-67 0.021692445441253483 1201 68-69 -0.018373524862823842 1201 70-71 -0.18223107221368906 1201 72-73 0.12558763161508324 1201 74-75 0.25965600890390306 1201 76-77 -0.07815778903027848 1201 78-79 -0.14519757270638678 1201 80-81 -0.1661457819179546 1201 82-83 -0.49459433007833553 1201 84-85 -0.7908133571458436 1201 86-87 -0.21201124900876778 1201 88-89 -0.7771326641998044 1201 90-91 -0.37098625435022825 1202 1 0.050336943412226276 1202 2 -0.11825391654000583 1202 3 0.07234189739023833 1202 4 -0.08852613046433788 1202 5 0.025586193771424348 1202 6 0.012520015725669964 1202 7 0.013624966993234011 1202 8 -0.10653808079998583 1202 9 0.06368125256253165 1202 10-11 0.031528833105390675 1202 12-13 0.0021329375249763416 1202 14-15 0.10844752386532974 1202 16-17 -0.032168827518212595 1202 18-19 0.019612768843558115 1202 20-21 0.04481942855513665 1202 22-23 -0.022476906649892214 1202 24-25 -0.004500795319863471 1202 26-27 0.015538451004651677 1202 28-29 0.0018164470735797522 1202 30-31 0.02694756895219541 1202 32-33 0.028166096287947084 1202 34-35 0.060816721217641145 1202 36-37 -0.11541983723917326 1202 38-39 -0.08531361318743791 1202 40-41 -0.09877628177029862 1202 42-43 -0.07672865322201261 1202 44-45 -0.045598261296312614 1202 46-47 -0.12446396265690396 1202 48-49 -0.049370423851193834 1202 50-51 -0.11910075645810991 1202 52-53 0.0458196234649364 1202 54-55 0.01647783254922075 1202 56-57 0.09842504371304983 1202 58-59 -0.010891706244969157 1202 60-61 0.052990644065275205 1202 62-63 0.10285594104349371 1202 64-65 0.049532737685332506 1202 66-67 -0.14979764695547715 1202 68-69 -0.03658114505719823 1202 70-71 -0.27209694605168977 1202 72-73 -0.05746482317650248 1202 74-75 0.07830259371284143 1202 76-77 -0.25911540649929066 1202 78-79 0.1895140053838631 1202 80-81 0.36766880774769817 1202 82-83 0.34018842986183273 1202 84-85 0.3170136817156788 1202 86-87 0.32995858240204967 1202 88-89 -0.5288422549186862 1202 90-91 0.6290137456497717 1203 1 0.07559505196570626 1203 2 0.004412082272295947 1203 3 0.07398131595746094 1203 4 -0.0316166418876449 1203 5 0.004090911901286631 1203 6 -0.1278200522326074 1203 7 -0.046803048780212464 1203 8 -0.021051698191648427 1203 9 0.01107082035986906 1203 10-11 0.045757357605637594 1203 12-13 0.034529507806020376 1203 14-15 -0.0671653951217337 1203 16-17 0.03306451306215763 1203 18-19 0.024339733437813038 1203 20-21 -0.10696891282804444 1203 22-23 0.007061820580510414 1203 24-25 -0.03603540326144383 1203 26-27 0.09343003888217538 1203 28-29 -0.03714163866324327 1203 30-31 -0.007073055148481444 1203 32-33 -0.02735965181753386 1203 34-35 -0.08521947471944458 1203 36-37 -0.0031565347530744248 1203 38-39 0.081038234163465 1203 40-41 0.022801185985230177 1203 42-43 -0.02606154361843238 1203 44-45 -0.04606050086795932 1203 46-47 0.077155081360047 1203 48-49 0.04460640496582613 1203 50-51 -0.026813031185497493 1203 52-53 0.10571457743751012 1203 54-55 0.16848505194865737 1203 56-57 0.09941850803789976 1203 58-59 -0.016706487057575714 1203 60-61 0.11088200449827212 1203 62-63 -0.050865943782063994 1203 64-65 0.02999900704417513 1203 66-67 0.06171878679250398 1203 68-69 0.13013540557153647 1203 70-71 -0.05893240248452969 1203 72-73 -0.16352677422829487 1203 74-75 -0.30723741714464836 1203 76-77 -0.062063154342176574 1203 78-79 -0.161314784594488 1203 80-81 -0.7422133227913221 1203 82-83 -0.2826255605992216 1203 84-85 -0.14642233581869846 1203 86-87 -0.4017487346711164 1203 88-89 -0.5747382448936236 1203 90-91 -0.259875143239114 1204 1 0.09452339018977796 1204 2 -0.006858382124960372 1204 3 -0.16983550556074078 1204 4 -0.03257957979230497 1204 5 -0.11100062226963558 1204 6 -0.08401109785415173 1204 7 -0.0838918923893388 1204 8 -0.2149200494751753 1204 9 -0.02216312477469984 1204 10-11 -0.11123062927197225 1204 12-13 -0.016095536446641745 1204 14-15 -0.04045016197904516 1204 16-17 -0.024117005453156537 1204 18-19 -0.06807949070487496 1204 20-21 -0.08040158191254676 1204 22-23 -0.1673392934064779 1204 24-25 0.00561952825511014 1204 26-27 -0.08302361186402152 1204 28-29 -0.09424369906714958 1204 30-31 -0.09584349987788698 1204 32-33 -0.14031759930388432 1204 34-35 -0.07836974958063792 1204 36-37 -0.20039274379423944 1204 38-39 -0.06626716578156078 1204 40-41 -0.032330507389026764 1204 42-43 -0.03331061186899831 1204 44-45 0.02925628131290381 1204 46-47 0.012657066602045575 1204 48-49 0.01639567699843525 1204 50-51 -0.1204325459974882 1204 52-53 0.030339818876797153 1204 54-55 -0.11584235709741364 1204 56-57 -0.13515018416251223 1204 58-59 -0.1783601586108361 1204 60-61 -0.1275820276563877 1204 62-63 -0.1687685707519293 1204 64-65 -0.2822168042367039 1204 66-67 -0.45434967223778955 1204 68-69 -0.261909788478377 1204 70-71 -0.13767014545947376 1204 72-73 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0.09564154716732531 2401 44-45 0.08986826768174438 2401 46-47 0.15650085987110884 2401 48-49 0.04384881201263369 2401 50-51 0.09804387304642148 2401 52-53 0.06453907224705802 2401 54-55 -0.030093101807445066 2401 56-57 0.08278352664743238 2401 58-59 0.024556651364683546 2401 60-61 0.1808406095465358 2401 62-63 0.047608600777842014 2401 64-65 0.21986988626310477 2401 66-67 0.050847628689247415 2401 68-69 0.06900803016548451 2401 70-71 0.16506173905835908 2401 72-73 0.16010814985653354 2401 74-75 0.14762213686959313 2401 76-77 0.3665019256142159 2401 78-79 0.4837158846047416 2401 80-81 0.3031945183047 2401 82-83 0.1458925296835787 2401 84-85 0.3037879804762724 2401 86-87 5.359921098957443E-4 2401 88-89 -0.11375913079213262 2401 90-91 0.6290137456497717 2402 1 0.002584682661463944 2402 2 0.033194915676503456 2402 3 0.15445496745724796 2402 4 0.21685413106712303 2402 5 0.06893275547853506 2402 6 0.09144526457090052 2402 7 0.10406280447941185 2402 8 0.20408061674734057 2402 9 0.15510536290017995 2402 10-11 0.1052117424801935 2402 12-13 0.14110632897641295 2402 14-15 0.09524604055260255 2402 16-17 0.09409751819774215 2402 18-19 0.053133034278474156 2402 20-21 0.015587758527153994 2402 22-23 0.12285391112383337 2402 24-25 0.05575943498395475 2402 26-27 0.010487380807532531 2402 28-29 0.04115936555643884 2402 30-31 0.07352358872969944 2402 32-33 -0.031179539521374977 2402 34-35 0.02387368846753901 2402 36-37 0.12024061868557823 2402 38-39 -0.46740231757254236 2402 40-41 0.08152429644287906 2402 42-43 0.07605471676071573 2402 44-45 0.08996477946168824 2402 46-47 0.07655878274270123 2402 48-49 0.04679277057057618 2402 50-51 -0.019989076614436385 2402 52-53 0.16668433189605736 2402 54-55 0.1277596200770148 2402 56-57 0.06716573721208619 2402 58-59 -0.017599182548657666 2402 60-61 -0.15676976044733237 2402 62-63 -0.0644253824514962 2402 64-65 0.12932996815872855 2402 66-67 0.14811205401162653 2402 68-69 0.1452092659806965 2402 70-71 0.2102589393628449 2402 72-73 0.11082853930562209 2402 74-75 0.16684151029291883 2402 76-77 0.239238974952535 2402 78-79 0.3258402376222378 2402 80-81 0.10599532666746114 2402 82-83 -0.3446023181205291 2402 84-85 -0.18613917760156085 2402 86-87 -0.07078675921285082 2402 88-89 0.5572061995508264 2402 90-91 0.6290137456497717 2403 1 0.018175129366461817 2403 2 -0.059062934832120106 2403 3 0.08200472420205074 2403 4 0.13204652381369186 2403 5 0.10819414424456397 2403 6 0.03381147421265496 2403 7 0.17652189337812985 2403 8 0.07258128017060983 2403 9 0.18961221827391483 2403 10-11 0.07473628914095798 2403 12-13 0.10962247227583788 2403 14-15 0.06797713298957575 2403 16-17 0.02147920771565026 2403 18-19 0.079298447812306 2403 20-21 0.09961916056165165 2403 22-23 0.019600925719153395 2403 24-25 0.06514245134839314 2403 26-27 0.0546558903165959 2403 28-29 0.025363257639583026 2403 30-31 0.011506782007018046 2403 32-33 0.03236679742589388 2403 34-35 0.02811326224335886 2403 36-37 0.14386314848797355 2403 38-39 0.072053042891568 2403 40-41 0.13015322669885876 2403 42-43 0.10822843371602175 2403 44-45 0.23336031404243585 2403 46-47 0.1634830022666378 2403 48-49 0.19049748122808552 2403 50-51 0.2537882081907554 2403 52-53 0.19306407905784084 2403 54-55 0.09727711499505176 2403 56-57 0.14485332539778994 2403 58-59 0.22045987454175275 2403 60-61 0.1889729501299513 2403 62-63 0.15606374710575466 2403 64-65 0.33765880064442655 2403 66-67 0.2808614287532194 2403 68-69 0.10231762740322381 2403 70-71 0.31259142427318665 2403 72-73 0.3048362879629778 2403 74-75 0.33596163971973425 2403 76-77 0.4166204789736909 2403 78-79 0.27537924785720236 2403 80-81 0.33278828401611094 2403 82-83 0.5754825475088907 2403 84-85 0.5576151854750719 2403 86-87 0.5075719034011286 2403 88-89 -0.1811450549294591 2403 90-91 0.6290137456497717 2404 1 -0.0628777571503889 2404 2 -0.06689359221405766 2404 3 0.022977887660978524 2404 4 0.05731726669571913 2404 5 0.09738553735389388 2404 6 -0.08681853347524537 2404 7 0.03403914670992236 2404 8 0.0731145447720607 2404 9 -0.003449564874038913 2404 10-11 -0.01136316915108182 2404 12-13 0.01491745647787468 2404 14-15 0.07690653764408495 2404 16-17 0.06876533528143369 2404 18-19 0.03298886104794008 2404 20-21 0.043884677450584775 2404 22-23 0.056664481971900216 2404 24-25 -0.07020399288768431 2404 26-27 0.013280667567528326 2404 28-29 0.04310041596140479 2404 30-31 0.0035500297661954505 2404 32-33 0.02049768291102083 2404 34-35 0.16055710943569323 2404 36-37 0.03957571596230025 2404 38-39 0.14468555337489164 2404 40-41 -0.06990329113259008 2404 42-43 0.061242967400069404 2404 44-45 0.19376163542870728 2404 46-47 0.19991686009905152 2404 48-49 0.022547519525133453 2404 50-51 0.061426332034415054 2404 52-53 0.015991782763634887 2404 54-55 0.007463128024447485 2404 56-57 0.03730034874119781 2404 58-59 -0.1033605666971269 2404 60-61 -0.04481239309588858 2404 62-63 0.16632315050156876 2404 64-65 0.09937453675313179 2404 66-67 0.12901882544631604 2404 68-69 0.12708466483623226 2404 70-71 0.1483679872616932 2404 72-73 0.0371161031435534 2404 74-75 0.014110260285029597 2404 76-77 0.33064893805709517 2404 78-79 0.16641819508166122 2404 80-81 0.20088014413013155 2404 82-83 -0.07281013541793868 2404 84-85 0.18445254915454257 2404 86-87 0.30569164610275834 2404 88-89 0.09371413605876455 2404 90-91 -2.898258981622959 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 17.0 31 356.0 32 2292.0 33 4134.0 34 5531.0 35 6836.0 36 9600.0 37 14372.0 38 37075.0 39 221004.0 40 246129.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.804785272935217 10.315412919798446 33.663167356662875 31.216634450603458 2 27.150071991872593 16.70485524882876 29.796230110874795 26.348842648423854 3 27.95927987050239 18.846945076788725 21.54140159971937 31.65237345298952 4 26.738479864655996 19.875910301710437 20.300504616823726 33.08510521680984 5 27.378294533987642 20.280407639774474 20.790688157034122 31.550609669203755 6 28.589594150683478 22.58754060502863 23.39781417969621 25.425051064591685 7 26.09976139407249 22.041633628454395 23.351773832274283 28.50683114519883 8 25.308305897914664 22.991672543509956 23.788609033408484 27.911412525166895 9 25.73966010530816 23.123947192452306 24.02648416175509 27.109908540484444 10-11 25.001552948226536 22.03377753742605 24.10139107621139 28.863278438136025 12-13 25.57815348974872 21.852173944817356 23.790801430904764 28.778871134529165 14-15 26.00256510507065 21.444844759987284 23.742111936508167 28.8104781984339 16-17 25.751261542059318 21.189978551044494 23.5739367785642 29.48482312833199 18-19 26.13666675192657 21.424930482729387 23.46349475468899 28.974908010655053 20-21 26.12396911642727 21.638049789347143 23.42531049829541 28.81267059593018 22-23 25.814292970077428 21.18440620740811 23.619154976925017 29.38214584558945 24-25 25.925557143013744 21.551176038556964 23.69817263668685 28.825094181742443 26-27 26.172658610823866 21.57529241101607 23.62691971805768 28.625129260102383 28-29 25.987857771866423 21.245519287616972 23.60801028965225 29.158612650864352 30-31 26.319640592970444 21.47699992326609 23.606274641634357 28.59708484212911 32-33 26.296268104808433 21.52805381942976 23.6570749827797 28.518603092982104 34-35 26.047627168814163 21.194442974228085 23.878273926791067 28.87965593016668 36-37 26.28022631631437 21.517014335491417 23.79806728417338 28.404692064020825 38-39 26.6022698415799 21.4368500901816 23.655143823916898 28.3057362443216 40-41 26.127577838353865 21.121079327835478 23.955695110589698 28.795647723220963 42-43 26.30968141258188 21.413466096512018 23.9067055393586 28.370146951547497 44-45 26.467881804830846 21.392750829881535 23.945938392336878 28.19342897295074 46-47 26.23079163676923 21.068919216533224 24.140126031017555 28.56016311567999 48-49 26.340264900866405 21.46967111661896 24.040994434091537 28.149069548423096 50-51 26.536043467888 21.392881024465808 24.0017607586047 28.069314749041496 52-53 26.276105933920217 21.158974547865483 24.249696121404103 28.315223396810197 54-55 26.512607941927545 21.468010214645595 24.089268532314577 27.93011331111228 56-57 26.57649054611228 21.45307883295323 24.017031602835605 27.953399018098885 58-59 26.369372275697533 21.118455109497265 24.12641013521793 28.385762479587267 60-61 26.82708747932842 21.573843092188245 23.900552176472235 27.6985172520111 62-63 26.740641770348685 21.493842251723823 23.900086230586272 27.865429747341224 64-65 26.52940649897055 21.314474979858563 23.97923193984424 28.17688658132665 66-67 26.715235853883147 21.669497595415944 24.033971144991302 27.58129540570961 68-69 26.88830150630651 21.53589551500417 23.879928948695113 27.695874029994204 70-71 26.730603377692386 21.196820995403982 24.000283839696074 28.072291787207565 72-73 26.891815784757956 21.408365972915547 23.962573279062028 27.73724496326447 74-75 26.739840306903034 21.550956407894837 23.790313372964828 27.918889912237297 76-77 26.721634373139114 21.47038614813797 23.838578168659247 27.969401310063667 78-79 27.080835807517783 21.53492801523724 23.82163464231696 27.562601534928017 80-81 27.50108332517927 21.318441689148575 23.55777967262612 27.62269531304603 82-83 27.487179487179485 20.658569500674766 23.40620782726046 28.44804318488529 84-85 27.56075651266115 21.11486076379616 23.01585374735973 28.308528976182963 86-87 28.84052347525486 20.14885886627394 22.896751208155493 28.113866450315705 88-89 27.866809116809115 19.687796771130103 22.827635327635328 29.61775878442545 90-91 25.973279701192357 20.385002154862807 25.140066082459416 28.501652061485416 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.5 9 2.0 10 4.5 11 5.5 12 6.0 13 9.5 14 17.5 15 27.5 16 40.5 17 60.0 18 91.5 19 128.0 20 171.5 21 242.5 22 337.5 23 489.5 24 701.0 25 933.0 26 1188.0 27 1495.0 28 1869.5 29 2320.5 30 2905.0 31 3546.5 32 4276.5 33 5037.0 34 5950.0 35 7053.0 36 8287.5 37 9549.5 38 10881.5 39 12436.5 40 14129.5 41 15897.5 42 17556.0 43 19332.0 44 21352.5 45 23597.0 46 25984.5 47 28408.5 48 30456.5 49 32365.5 50 34431.5 51 36258.0 52 37613.0 53 38649.5 54 39518.0 55 39994.5 56 40330.0 57 40386.0 58 40045.0 59 39502.0 60 38763.5 61 37631.0 62 36246.0 63 34550.5 64 32330.5 65 30043.5 66 27556.5 67 25229.0 68 22839.5 69 20122.0 70 17576.5 71 15097.0 72 12696.0 73 10415.5 74 8399.5 75 6846.5 76 5546.0 77 4262.5 78 3266.0 79 2495.0 80 1822.0 81 1351.0 82 1003.0 83 734.0 84 546.5 85 405.5 86 280.0 87 192.5 88 149.0 89 102.5 90 66.0 91 47.5 92 32.5 93 22.0 94 15.5 95 11.0 96 9.5 97 9.0 98 7.5 99 3.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011327387064123973 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.05499028721850016 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 3.5806872054884776E-4 66-67 0.0 68-69 4.711602793038135E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0011203728600878372 80-81 0.001397819401733296 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 9996.0 32-33 21485.0 34-35 23464.0 36-37 24939.0 38-39 26027.0 40-41 27386.0 42-43 27750.0 44-45 27567.0 46-47 27597.0 48-49 26901.0 50-51 26340.0 52-53 25550.0 54-55 23947.0 56-57 22931.0 58-59 21908.0 60-61 20410.0 62-63 18954.0 64-65 17910.0 66-67 16277.0 68-69 14943.0 70-71 13734.0 72-73 12642.0 74-75 11498.0 76-77 10211.0 78-79 9051.0 80-81 8246.0 82-83 7399.0 84-85 6615.0 86-87 5921.0 88-89 5071.0 90-91 4676.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.94605832218207 #Duplication Level Percentage of deduplicated Percentage of total 1 99.94950728509134 99.89559284389101 2 0.047344705533917626 0.09463833401078936 3 0.0028179430902079148 0.008449269133275308 4 3.3006628452091974E-4 0.0013195529649165515 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0014615983308547062 1.8269979135683827E-4 0.0 0.0 1.8269979135683827E-4 2 0.0014615983308547062 1.8269979135683827E-4 0.0 0.0 1.8269979135683827E-4 3 0.0016442981222115445 1.8269979135683827E-4 0.0 0.0 1.8269979135683827E-4 4 0.0016442981222115445 1.8269979135683827E-4 0.0 0.0 1.8269979135683827E-4 5 0.0016442981222115445 1.8269979135683827E-4 0.0 0.0 1.8269979135683827E-4 6 0.0016442981222115445 1.8269979135683827E-4 0.0 0.0 1.8269979135683827E-4 7 0.0029231966617094124 1.8269979135683827E-4 0.0 0.0 1.8269979135683827E-4 8 0.00639449269748934 1.8269979135683827E-4 0.0 0.0 1.8269979135683827E-4 9 0.00639449269748934 1.8269979135683827E-4 0.0 0.0 1.8269979135683827E-4 10-11 0.00639449269748934 1.8269979135683827E-4 0.0 0.0 1.8269979135683827E-4 12-13 0.00639449269748934 1.8269979135683827E-4 0.0 0.0 2.740496870352574E-4 14-15 0.00639449269748934 2.740496870352574E-4 0.0 0.0 3.6539958271367655E-4 16-17 0.00639449269748934 3.6539958271367655E-4 0.0 0.0 3.6539958271367655E-4 18-19 0.006485842593167758 3.6539958271367655E-4 0.0 0.0 3.6539958271367655E-4 20-21 0.006577192488846178 3.6539958271367655E-4 0.0 0.0 3.6539958271367655E-4 22-23 0.006577192488846178 3.6539958271367655E-4 0.0 0.0 3.6539958271367655E-4 24-25 0.006759892280203016 3.6539958271367655E-4 0.0 0.0 3.6539958271367655E-4 26-27 0.007125291862916693 3.6539958271367655E-4 0.0 0.0 3.6539958271367655E-4 28-29 0.0073993415499519506 3.6539958271367655E-4 0.0 0.0 3.6539958271367655E-4 30-31 0.007582041341308788 3.6539958271367655E-4 0.0 0.0 3.6539958271367655E-4 32-33 0.008221490611057721 3.6539958271367655E-4 0.0 0.0 3.6539958271367655E-4 34-35 0.008221490611057721 3.6539958271367655E-4 0.0 0.0 3.6539958271367655E-4 36-37 0.008769589985128237 3.6539958271367655E-4 0.0 0.0 4.567494783920957E-4 38-39 0.008769589985128237 3.6539958271367655E-4 0.0 0.0 5.480993740705148E-4 40-41 0.009043639672163494 3.6539958271367655E-4 0.0 0.0 5.480993740705148E-4 42-43 0.009226339463520333 3.6539958271367655E-4 0.0 0.0 5.480993740705148E-4 44-45 0.00940903925487717 3.6539958271367655E-4 0.0 0.0 5.480993740705148E-4 46-47 0.009683088941912428 3.6539958271367655E-4 0.0 0.0 7.307991654273531E-4 48-49 0.009683088941912428 3.6539958271367655E-4 0.0 0.0 7.307991654273531E-4 50-51 0.009683088941912428 3.6539958271367655E-4 9.134989567841914E-5 0.0 7.307991654273531E-4 52-53 0.009865788733269267 3.6539958271367655E-4 1.8269979135683827E-4 0.0 7.307991654273531E-4 54-55 0.009865788733269267 3.6539958271367655E-4 1.8269979135683827E-4 0.0 7.307991654273531E-4 56-57 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