##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-23B_S8_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1012997
Sequences flagged as poor quality	0
Sequence length	61
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.478920470643054	40.0	40.0	40.0	40.0	40.0
2	39.20278934685888	40.0	40.0	40.0	40.0	40.0
3	39.140354808553234	40.0	40.0	40.0	40.0	40.0
4	39.012135277794506	40.0	40.0	40.0	40.0	40.0
5	39.044411780094116	40.0	40.0	40.0	40.0	40.0
6	39.137628245690756	40.0	40.0	40.0	40.0	40.0
7	39.163010354423555	40.0	40.0	40.0	40.0	40.0
8	39.183101233271174	40.0	40.0	40.0	40.0	40.0
9	39.201667922017535	40.0	40.0	40.0	40.0	40.0
10	39.21146064598415	40.0	40.0	40.0	40.0	40.0
11	39.202165455573905	40.0	40.0	40.0	40.0	40.0
12	39.211010496576	40.0	40.0	40.0	40.0	40.0
13	39.029880641304956	40.0	40.0	40.0	40.0	40.0
14	39.20736586584166	40.0	40.0	40.0	40.0	40.0
15	39.11397565836819	40.0	40.0	40.0	40.0	40.0
16	39.30083109821648	40.0	40.0	40.0	40.0	40.0
17	39.24800172162405	40.0	40.0	40.0	40.0	40.0
18	39.3302092701163	40.0	40.0	40.0	40.0	40.0
19	39.280207147701326	40.0	40.0	40.0	40.0	40.0
20	39.29013412675457	40.0	40.0	40.0	40.0	40.0
21	39.27218343193514	40.0	40.0	40.0	40.0	40.0
22	39.333648569541666	40.0	40.0	40.0	40.0	40.0
23	39.327765037803665	40.0	40.0	40.0	40.0	40.0
24	39.328349442298446	40.0	40.0	40.0	40.0	40.0
25	39.28550232626553	40.0	40.0	40.0	40.0	40.0
26	39.24473221539649	40.0	40.0	40.0	40.0	40.0
27	39.26784383369348	40.0	40.0	40.0	40.0	40.0
28	39.280791552196106	40.0	40.0	40.0	40.0	40.0
29	39.28046381183755	40.0	40.0	40.0	40.0	40.0
30	39.28532068703066	40.0	40.0	40.0	40.0	40.0
31	39.22532050934011	40.0	40.0	40.0	40.0	40.0
32	39.2558280034393	40.0	40.0	40.0	40.0	40.0
33	39.257194246379804	40.0	40.0	40.0	40.0	40.0
34	39.231010555806186	40.0	40.0	40.0	40.0	40.0
35	39.260191293755064	40.0	40.0	40.0	40.0	40.0
36	39.225099383315055	40.0	40.0	40.0	40.0	40.0
37	39.238923708559845	40.0	40.0	40.0	40.0	40.0
38	39.223504116991464	40.0	40.0	40.0	40.0	40.0
39	37.98649156907671	40.0	40.0	40.0	40.0	40.0
40	39.21654259588133	40.0	40.0	40.0	40.0	40.0
41	39.246287994929894	40.0	40.0	40.0	40.0	40.0
42	39.2095810747712	40.0	40.0	40.0	40.0	40.0
43	39.2326729496731	40.0	40.0	40.0	40.0	40.0
44	39.199314509322335	40.0	40.0	40.0	40.0	40.0
45	39.04385106767345	40.0	40.0	40.0	40.0	40.0
46	39.06997157938276	40.0	40.0	40.0	40.0	40.0
47	39.13838244338335	40.0	40.0	40.0	40.0	40.0
48	39.24599579268251	40.0	40.0	40.0	40.0	40.0
49	39.21391277565481	40.0	40.0	40.0	40.0	40.0
50	39.20745273678007	40.0	40.0	40.0	40.0	40.0
51	39.16336573553525	40.0	40.0	40.0	40.0	40.0
52	39.18809236355093	40.0	40.0	40.0	40.0	40.0
53	39.09329247766775	40.0	40.0	40.0	40.0	40.0
54	38.985051288404605	40.0	40.0	40.0	40.0	40.0
55	39.03742656690987	40.0	40.0	40.0	40.0	40.0
56	39.02438802878982	40.0	40.0	40.0	40.0	40.0
57	38.91547161541446	40.0	40.0	40.0	40.0	40.0
58	38.85962544805167	40.0	40.0	40.0	40.0	40.0
59	38.79790759498794	40.0	40.0	40.0	40.0	40.0
60	38.74215619592161	40.0	40.0	40.0	40.0	40.0
61	38.63598016578529	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.008626092329265589
1101	2	-0.07393225095782441
1101	3	0.014836647953430315
1101	4	0.039977090339590404
1101	5	0.0509208150275029
1101	6	0.018687321352054198
1101	7	-0.055081185056899074
1101	8	0.058350196893798056
1101	9	0.03894329155944831
1101	10	-0.00814476937775055
1101	11	0.07582905896375536
1101	12	0.026440216332446198
1101	13	0.015058418494334092
1101	14	-0.07527548353439073
1101	15	-0.008642308376693109
1101	16	-0.10085500379810952
1101	17	-0.05916181506206186
1101	18	-0.09673276089549887
1101	19	0.05060600466012488
1101	20	2.6739954650878417E-4
1101	21	0.019900265477147627
1101	22	0.004565513620015338
1101	23	0.02705731472698858
1101	24	0.032468798291077405
1101	25	0.05018186190213214
1101	26	0.06712025152043566
1101	27	0.042660905021286055
1101	28	-0.0573700263621717
1101	29	0.028531893902332683
1101	30	0.07829805546255386
1101	31	-0.021552971813555644
1101	32	0.16675363174522317
1101	33	0.107267727821025
1101	34	-0.022829191174231767
1101	35	-0.013000664479896784
1101	36	0.010590447438922013
1101	37	0.12811064003292216
1101	38	0.08096018746301326
1101	39	1.2522887720185736
1101	40	0.08636246376978818
1101	41	-0.06929346818633775
1101	42	0.125670269191275
1101	43	0.07473535543233822
1101	44	-0.025974929874330144
1101	45	-0.03287335857068996
1101	46	0.010905646345349851
1101	47	0.003798250393991509
1101	48	0.04568595521872254
1101	49	0.05714218991221287
1101	50	-0.0022941499697566314
1101	51	0.062463606612041644
1101	52	0.06088170243486957
1101	53	0.1568348500295329
1101	54	0.111817246957429
1101	55	-0.03228076571048888
1101	56	0.023082185868595673
1101	57	0.0335946290487783
1101	58	-0.05757658885952566
1101	59	-0.04878339651643415
1101	60	0.009793533331922788
1101	61	-0.1026474065995302
1102	1	-0.020381483375558673
1102	2	-0.1560049656537359
1102	3	-0.034589970772202605
1102	4	-0.10107253667823102
1102	5	-0.17474597621157528
1102	6	-0.12924744603411398
1102	7	-0.14464410611745393
1102	8	-0.15901744105080695
1102	9	-0.07613492211860518
1102	10	-0.19576861640592824
1102	11	0.004596078379258017
1102	12	-0.20586179671128946
1102	13	-0.15345749079528304
1102	14	-0.23083857017566345
1102	15	-0.1532495996540746
1102	16	-0.1675592743105767
1102	17	-0.163284233376217
1102	18	-0.1994237570956514
1102	19	-0.1276161071749442
1102	20	-0.15075150218907396
1102	21	-0.2682790507054307
1102	22	-0.17741156660384405
1102	23	-0.15981019980581124
1102	24	-0.17567467817672622
1102	25	-0.1729683345121984
1102	26	-0.143669034633227
1102	27	-0.032195507865843354
1102	28	-0.07274543422075652
1102	29	-0.18225739225132997
1102	30	-0.1259523397260125
1102	31	-0.10695316261825383
1102	32	-0.17826745658435783
1102	33	0.025361754457861707
1102	34	-0.04453303226006966
1102	35	-0.19549731224405065
1102	36	-0.2280202684191437
1102	37	-0.06743516121914439
1102	38	-0.08878751858632938
1102	39	1.0598862963546907
1102	40	-0.1282475588157297
1102	41	-0.08249001946490608
1102	42	-0.1248607110388491
1102	43	-0.2072076027832921
1102	44	-0.04914965390001669
1102	45	-0.12424915639508072
1102	46	-0.10141727319866334
1102	47	-0.1879488660641897
1102	48	-0.08294124907461509
1102	49	-0.1488750444100404
1102	50	-0.09515823992187222
1102	51	-0.07274194947223123
1102	52	-0.05793174921903699
1102	53	-0.09168827053257189
1102	54	-0.02032390519036653
1102	55	-0.15984717015609817
1102	56	-0.18039021205746764
1102	57	-0.19281534107705767
1102	58	-0.16750593850643014
1102	59	-0.1803175483137167
1102	60	-0.18153731010067276
1102	61	-0.11550892772925181
1103	1	0.03869708981656572
1103	2	0.02187052162321379
1103	3	0.1567627385515422
1103	4	0.1535660250641726
1103	5	0.03643717106385225
1103	6	0.043347793677128266
1103	7	-0.04005664513157825
1103	8	-0.002724080411276475
1103	9	0.0031478770421387026
1103	10	0.06896930409329372
1103	11	0.021719110705035405
1103	12	0.15979337990846432
1103	13	0.09954073174432665
1103	14	-0.0056541107075673835
1103	15	0.028566793704698057
1103	16	-0.03657057549617804
1103	17	0.04676299178876775
1103	18	0.04511505242886216
1103	19	-0.029114702027101202
1103	20	0.0939183298719044
1103	21	0.04932469268319295
1103	22	-0.0625791662650741
1103	23	-0.017057408445431577
1103	24	0.00578720675754596
1103	25	-0.020790576669782013
1103	26	-0.005473309391028636
1103	27	0.12520898683669657
1103	28	-0.012661961454654147
1103	29	0.12123801753345731
1103	30	0.07481549955301148
1103	31	0.05665385239917242
1103	32	-0.10873462274985002
1103	33	-0.005634715212686103
1103	34	-0.01627190395142719
1103	35	0.05193894460339976
1103	36	0.0339319477011486
1103	37	-0.06725456258779872
1103	38	-0.022297712670841463
1103	39	1.1029498235954733
1103	40	0.0463228554692634
1103	41	0.0816556437579905
1103	42	0.04984072578261589
1103	43	0.006998899357625987
1103	44	-0.04789648039056971
1103	45	-0.08023424028590398
1103	46	0.05900624829758527
1103	47	-0.006117914645813016
1103	48	0.04324761814112321
1103	49	-0.037599925609384854
1103	50	0.0532631450807699
1103	51	0.06775906418184974
1103	52	0.10586452057328444
1103	53	-0.05557444617949869
1103	54	0.06572445707711694
1103	55	0.002313875427354617
1103	56	-0.03638975564262381
1103	57	0.04574482748166275
1103	58	0.08209041961790575
1103	59	0.05300132532527613
1103	60	0.04619559672085671
1103	61	0.1383002180766013
1104	1	0.014086861241011661
1104	2	-0.1031918724700489
1104	3	0.10455883969971325
1104	4	0.08335939007184123
1104	5	0.04579346581918742
1104	6	-0.01194994213199152
1104	7	0.06302720085714952
1104	8	0.04738547402120474
1104	9	0.04932910134136392
1104	10	0.07833192975940051
1104	11	0.10040718772056323
1104	12	0.063207422462078
1104	13	0.060894873990001486
1104	14	0.0016584869145859216
1104	15	0.10787134315653191
1104	16	0.06325285144561121
1104	17	-0.04306274000021659
1104	18	0.041415822129124535
1104	19	-0.0020112816536368427
1104	20	0.11147473680306774
1104	21	0.050913355604734534
1104	22	-0.020484149186458467
1104	23	-0.042943315541293714
1104	24	-0.03391868286895772
1104	25	0.01883340679862755
1104	26	0.06062654292069425
1104	27	0.007277176680922537
1104	28	0.0669947449088042
1104	29	-0.05698345157416895
1104	30	0.08622815326307176
1104	31	0.059797545278776454
1104	32	-0.014363297027458088
1104	33	-0.010001856598741199
1104	34	0.0561897223210579
1104	35	-0.06295798520163487
1104	36	-0.033068080985785286
1104	37	0.026861152612219996
1104	38	-0.02486924948953373
1104	39	1.276485627368892
1104	40	0.006955273887072622
1104	41	-0.12369456936618661
1104	42	0.03423740482423199
1104	43	-0.01341116560345057
1104	44	0.08143017778871808
1104	45	-0.07397930044439249
1104	46	0.02802910189626573
1104	47	0.03805838934869854
1104	48	-0.018832897195011356
1104	49	-0.018788019935996658
1104	50	0.10794638686319757
1104	51	-0.07300477998673927
1104	52	0.06250094724897082
1104	53	-0.11497223807652546
1104	54	0.0752912546155784
1104	55	0.05702085812517055
1104	56	0.14787219370415272
1104	57	0.06832085529018883
1104	58	0.0445572194315389
1104	59	0.04373959854957832
1104	60	0.04100504125268145
1104	61	0.016518818490283138
1201	1	0.034380304161572894
1201	2	0.11720311921479976
1201	3	0.047382031262813484
1201	4	0.02690254878207554
1201	5	0.019201281190717623
1201	6	0.049985095783753763
1201	7	0.027078161638549147
1201	8	0.07092692407154289
1201	9	0.07132965083085452
1201	10	0.07384863919110529
1201	11	0.010315764707826247
1201	12	-0.08607197935162247
1201	13	0.15307668705290212
1201	14	0.04895225117971336
1201	15	0.12558703962474738
1201	16	-0.020076938360098495
1201	17	-0.02668096324634206
1201	18	0.08924393094357441
1201	19	0.028339566414459227
1201	20	-0.05906777242223882
1201	21	-0.0159193229041108
1201	22	0.04174640922495598
1201	23	-0.042297908098717585
1201	24	0.11669929864062567
1201	25	0.02792464244127757
1201	26	0.12044400756065698
1201	27	0.09846835533587495
1201	28	0.05925451557946104
1201	29	0.05337267780633681
1201	30	-0.010899065203659575
1201	31	0.10627930776146144
1201	32	0.07501980879198555
1201	33	-0.017881885374123385
1201	34	-0.06097114509567092
1201	35	0.11104960862660818
1201	36	0.02813456316540197
1201	37	0.08969673195957029
1201	38	0.03153905032591098
1201	39	1.2537130742718006
1201	40	0.0666488606845661
1201	41	0.05858721000893041
1201	42	0.09967713718595661
1201	43	0.0909281509520028
1201	44	0.08828345729926923
1201	45	0.11016530654926981
1201	46	0.126609846270064
1201	47	0.09211728180699907
1201	48	0.10897214471084027
1201	49	0.04862249628316562
1201	50	0.07847239198724054
1201	51	0.11720090965654606
1201	52	0.06973634524537431
1201	53	0.12358192498594178
1201	54	0.0825814073950113
1201	55	0.09466574229065117
1201	56	0.07194789122087286
1201	57	0.07891571164140743
1201	58	0.040024222393540754
1201	59	0.13936047798517848
1201	60	0.09636406855920399
1201	61	0.06234041692219705
1202	1	-0.010325501320473052
1202	2	0.012415023441349149
1202	3	-0.06018110376282948
1202	4	-0.015244224658260919
1202	5	0.005654323644591841
1202	6	0.1174777402001439
1202	7	0.05609461777505942
1202	8	0.06904512460079815
1202	9	-0.01367495181388989
1202	10	0.021149798212000803
1202	11	0.025716079494138455
1202	12	-0.03486597112259204
1202	13	-0.06996113534842863
1202	14	-0.06712894754500098
1202	15	-0.08370549832545038
1202	16	-0.009494891172899145
1202	17	0.04337148192068696
1202	18	-0.0016266895774919021
1202	19	0.0439766435524831
1202	20	-0.03990923965697846
1202	21	0.019455124826563974
1202	22	0.028971235448693733
1202	23	0.055198983434529225
1202	24	-0.06106156754060521
1202	25	0.027410376164347383
1202	26	-0.012771713305752996
1202	27	-0.054613879224120865
1202	28	0.050151464381521293
1202	29	-0.08239669413924844
1202	30	0.05430743884263478
1202	31	2.8664508823794677E-4
1202	32	-0.04467816546780057
1202	33	0.016251097427932848
1202	34	0.015159481613508774
1202	35	0.08459064711394149
1202	36	-0.18604388877011502
1202	37	-0.24101030730031425
1202	38	-0.16778487016652832
1202	39	1.1078028940672127
1202	40	-0.22941707916780274
1202	41	-0.2746581061514419
1202	42	-0.18387268380094923
1202	43	-0.20876378894264036
1202	44	-0.1953370849095819
1202	45	-0.1402616151750209
1202	46	-0.14362824210455472
1202	47	-0.06254464961263295
1202	48	-0.03422449295627672
1202	49	-0.11712652409338631
1202	50	-0.0015571264358342773
1202	51	0.0718985860416197
1202	52	-0.1009846845171154
1202	53	-0.07206987292816791
1202	54	-0.10601517992167686
1202	55	-0.08752980573155611
1202	56	0.002614957616181357
1202	57	-0.08376385396117314
1202	58	-0.0024607829778133805
1202	59	-0.01606093786136853
1202	60	-0.04313661655263701
1202	61	-0.07998142997788449
1203	1	0.06949190003960837
1203	2	0.10053445751305645
1203	3	0.015975646448325165
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2403	8	0.14070442612549527
2403	9	-0.011161568691441914
2403	10	0.024812099976571744
2403	11	0.07257297623917935
2403	12	0.10386526279427244
2403	13	-9.18837147288798E-4
2403	14	0.0679376116255952
2403	15	0.04144437030034709
2403	16	0.10634379273980699
2403	17	-0.017620871343517308
2403	18	-0.02876529484563406
2403	19	0.1010069083633951
2403	20	0.08768756860352767
2403	21	-0.03392430763135934
2403	22	0.0026532863534995954
2403	23	0.13523807435314694
2403	24	0.1033255535757931
2403	25	-0.04099794042463856
2403	26	0.09515268112414788
2403	27	0.04128384842837107
2403	28	0.04758548016462072
2403	29	0.07822727349011416
2403	30	-0.11767361922640873
2403	31	-0.007979241183846852
2403	32	0.0634044277424266
2403	33	0.05076492983491221
2403	34	0.10077033357524101
2403	35	0.005370234883514513
2403	36	0.04941719838377168
2403	37	0.016107797383376976
2403	38	0.13259941444573542
2403	39	1.2073146728562705
2403	40	0.031162810326392787
2403	41	0.040420867668437666
2403	42	0.09595881076818813
2403	43	-0.1103412087561324
2403	44	0.06335074717190281
2403	45	0.0776042081339483
2403	46	0.11249914271733985
2403	47	0.08068101165332564
2403	48	0.04105479215925101
2403	49	0.17836348575250582
2403	50	0.07509492859598765
2403	51	0.05098826675494905
2403	52	0.07834787087371353
2403	53	0.09532856197174766
2403	54	0.08413856754047089
2403	55	-0.04551023219196537
2403	56	0.06810292472032842
2403	57	0.09142072940948509
2403	58	0.10848995411974016
2403	59	0.10249085513394363
2403	60	0.00979669534476102
2403	61	0.06739576278080506
2404	1	-0.04336812095527165
2404	2	-0.14657277521125422
2404	3	-0.23323843883133577
2404	4	0.03626458662708387
2404	5	0.016422474077543825
2404	6	-0.04387435411284457
2404	7	-0.05376544180567322
2404	8	-0.01030317821119553
2404	9	0.06562062468839258
2404	10	-0.15407410885547534
2404	11	-0.09306191637882932
2404	12	-0.034259618560938065
2404	13	0.1089727866022514
2404	14	-0.03846980156742319
2404	15	0.1073458882566598
2404	16	-0.040040201047816026
2404	17	-0.09873311269787166
2404	18	0.018145676563584345
2404	19	-0.19613417756392693
2404	20	-0.029349597812426964
2404	21	-0.07507595853133608
2404	22	-0.11742815676075224
2404	23	0.09494743100420777
2404	24	-0.035285592043955205
2404	25	-0.15427250384255586
2404	26	0.006022819455260731
2404	27	-0.1086655366277327
2404	28	-0.07758041754030387
2404	29	0.016340510743205527
2404	30	-0.029575326604380336
2404	31	-0.06489266031453411
2404	32	0.09740725594723187
2404	33	0.015625571662745585
2404	34	0.0850122553768955
2404	35	0.08142467188092439
2404	36	0.049767341454526104
2404	37	0.08826310018537953
2404	38	-0.017006797600743084
2404	39	1.2455503781834096
2404	40	0.08005040907063687
2404	41	0.04598256644453613
2404	42	0.12369186398706233
2404	43	0.050544202208925526
2404	44	-0.009797994342555683
2404	45	0.12018718158662267
2404	46	0.08983906398844965
2404	47	0.021206300060107708
2404	48	0.07614125986757614
2404	49	0.11420776310681902
2404	50	0.14369427021221526
2404	51	0.03516991802803204
2404	52	0.07087432533072047
2404	53	0.12697673952626332
2404	54	0.0076819170156454675
2404	55	0.043420777732990246
2404	56	0.021526926248824907
2404	57	0.06593572590600161
2404	58	-0.10010511525901933
2404	59	-0.09542362057600684
2404	60	-0.04663332954655175
2404	61	0.10447197793921248
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	7.0
17	37.0
18	86.0
19	139.0
20	200.0
21	281.0
22	393.0
23	568.0
24	843.0
25	1158.0
26	1483.0
27	1800.0
28	2564.0
29	3087.0
30	3892.0
31	4850.0
32	6446.0
33	7747.0
34	9704.0
35	12615.0
36	18525.0
37	28226.0
38	67483.0
39	421412.0
40	419451.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.700270583229763	11.41207723221293	33.761304327653484	30.126347856903823
2	28.074418237208132	16.568920313433498	29.276407068547535	26.080254380810842
3	28.439697688835892	19.748449154784424	21.314990384957095	30.496862771422588
4	26.855710047927182	19.810265598546884	21.353017537105316	31.981006816420614
5	27.383397976499435	19.956031459125743	20.733921225827913	31.92664933854691
6	29.837798137605542	22.06531707398936	22.575881271119265	25.521003517285834
7	27.503832686572615	21.48732918261357	22.329681134297534	28.67915699651628
8	25.60560396526347	23.444886806180076	22.56521983776852	28.38428939078793
9	26.241933589141926	23.518430952905092	22.739948884350103	27.499686573602883
10	26.7234749954837	21.681702907313642	22.605693797711147	28.989128299491508
11	25.660293169673746	22.526917651286233	22.457914485432827	29.354874693607186
12	27.91656836101193	22.004408700124483	22.067686281400636	28.011336657462955
13	25.875989761075303	20.809933296939676	22.47499252218911	30.839084419795913
14	26.44973282250589	22.051299263472647	22.26235615702712	29.236611756994346
15	26.811530537602778	21.494634238798337	22.073708016904295	29.62012720669459
16	26.468192896918747	22.034221226716365	21.92425051604299	29.573335360321895
17	26.53719606277215	20.96442536354994	21.958801457457426	30.539577116220485
18	28.34509875152641	21.895820027107682	21.61951121276766	28.13957000859825
19	26.33403652725526	21.008453134609482	23.103720938956386	29.55378939917887
20	26.40175637242756	21.37883922657224	22.051200546497178	30.168203854503023
21	26.97174818879029	21.93520810032014	22.855645179600728	28.23739853128884
22	27.392085070340784	21.38426866022308	22.182000538994686	29.041645730441452
23	26.43986112495891	22.590984968366147	22.00430998314901	28.964843923525933
24	27.11567753902529	23.33461994458029	21.77696478864202	27.7727377277524
25	26.33561599886278	21.601149855330274	22.073905450855236	29.989328694951713
26	26.644698849058784	22.0226713405864	23.066603356179733	28.266026454175087
27	26.975104565956265	22.75692820413091	21.750804790142517	28.517162439770306
28	26.612220964129214	22.399868903856575	21.9955241723322	28.992385959682014
29	26.753781106952935	23.789902635447095	21.834714219291865	27.621602038308108
30	28.424269765853204	23.672824302539887	21.445670618965305	26.457235312641597
31	26.851807063594464	23.038863886072715	22.803226465626256	27.306102584706572
32	27.245095493866224	23.557226724264734	21.256430176989667	27.941247604879383
33	27.54302332583413	25.299581341307032	21.2929554579135	25.864439874945333
34	27.190998591308762	24.095925259403533	21.14448512680689	27.568591022480817
35	27.52268762888735	24.372332790719025	20.782983562636414	27.32199601775721
36	28.131771367536135	26.313996981234887	20.200849558290894	25.35338209293808
37	28.50432923295923	25.094842334182626	20.442903582142886	25.95792485071525
38	27.783399161103144	25.665130301471773	20.8389560877278	25.71251444969728
39	29.587133990358645	25.917508331719645	19.489599364037545	25.005758313884165
40	27.86187915660165	26.045388090981515	19.427895640362213	26.664837112054624
41	28.151119894728215	26.30777781178029	20.14843084431642	25.39267144917507
42	28.356352486729968	26.954275284132134	19.928588139945134	24.76078408919276
43	27.804820744780095	27.818641121345866	19.01407407919273	25.362464054681304
44	27.990211224712414	26.89573611767853	19.666593287048233	25.44745937056082
45	28.20501936333474	27.38556974995977	18.395513510898848	26.013897375806643
46	27.55092068387172	27.348945752060473	19.470640090740645	25.62949347332717
47	28.783500839587877	27.48083163128815	18.18060665530105	25.55506087382292
48	28.874320457020108	27.86543296771856	18.02996455073411	25.230282024527217
49	28.360893467601585	27.829401271672076	18.028483796102062	25.781221464624277
50	28.259511133794078	27.93019130362676	17.933616782675564	25.876680779903594
51	28.18369649663326	28.24154464425857	17.884850596793473	25.6899082623147
52	27.722392070262796	28.1871515907747	17.754248038246907	26.336208300715597
53	27.786360670367237	28.158030083011106	17.61584683863822	26.43976240798344
54	27.5169620443101	28.403736634955486	17.619104498828722	26.46019682190569
55	26.872044043565772	28.473035951735298	17.62196729111735	27.032952713581583
56	26.899882230648263	28.208968042353533	17.588304802482142	27.302844924516062
57	26.757631068996258	28.285078830440764	17.601631594170566	27.355658506392416
58	26.115575860540552	28.363953693841147	17.697584494327227	27.82288595129107
59	26.21676076039712	28.18231445897668	17.628976196375707	27.971948584250494
60	25.946276247609816	28.256944492431863	17.720980417513577	28.075798842444744
61	25.75476531519837	28.060300277295987	17.880901917774683	28.304032489730968
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.5
2	4.0
3	6.0
4	9.0
5	12.0
6	11.0
7	8.5
8	7.0
9	8.5
10	10.0
11	12.5
12	21.0
13	27.0
14	30.0
15	33.0
16	33.5
17	60.0
18	86.0
19	96.0
20	127.5
21	149.0
22	180.5
23	212.0
24	298.0
25	503.0
26	622.0
27	809.5
28	997.0
29	1309.5
30	2004.5
31	2387.0
32	2984.5
33	3582.0
34	4581.0
35	6572.5
36	7565.0
37	9124.0
38	10683.0
39	12481.5
40	16148.5
41	18017.0
42	20542.0
43	26145.5
44	29224.0
45	33147.0
46	37070.0
47	41564.5
48	50452.0
49	54845.0
50	59942.5
51	65040.0
52	69562.0
53	78081.5
54	82079.0
55	84334.0
56	86589.0
57	86390.0
58	83489.5
59	80788.0
60	76599.0
61	68948.5
62	65487.0
63	55079.5
64	44672.0
65	38486.0
66	27658.5
67	23017.0
68	19359.0
69	15701.0
70	13071.0
71	8701.0
72	6961.0
73	5662.0
74	4363.0
75	3519.0
76	2129.5
77	1584.0
78	1226.5
79	869.0
80	671.0
81	360.5
82	248.0
83	206.0
84	110.0
85	56.0
86	47.5
87	39.0
88	28.0
89	11.0
90	5.0
91	7.5
92	10.0
93	8.5
94	9.0
95	11.0
96	10.0
97	9.0
98	9.0
99	6.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03376120561067802
3	9.871697546981877E-5
4	1.9743395093963753E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.1398908387685256
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1012997.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.63478056149873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.13449334072487	65.40762353718505
2	13.886204392328864	22.116496792304577
3	2.819870400945685	6.736792817735253
4	0.6612255811322292	2.106262162204582
5	0.23114750249254545	0.920369031916617
6	0.10354192171305335	0.4947322928720931
7	0.05761527909116744	0.32117260751902377
8	0.034771168690322006	0.22151955108165186
9	0.021173423503891783	0.15175268410212917
>10	0.0491465606990551	0.5278826558532181
>50	2.701428927629953E-4	0.014198428190383543
>100	4.0521433914449286E-4	0.07421905644973216
>500	0.0	0.0
>1k	0.0	0.0
>5k	1.3507144638149764E-4	0.9069783825857124
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCTTATCTGGGGGGGGGGGGGGG	8432	0.8323815371615119	TruSeq Adapter, Index 16 (97% over 37bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0171797152410125	0.0	0.0	0.0	0.0
2	1.020239941480577	0.0	0.0	0.0	0.0
3	1.0222142809899732	0.0	0.0	0.0	0.0
4	1.023497601671081	0.0	0.0	0.0	0.0
5	1.0269526958125246	0.0	0.0	0.0	9.871697546981877E-5
6	1.031098808782257	0.0	0.0	0.0	9.871697546981877E-5
7	1.0351462047765196	0.0	0.0	0.0	9.871697546981877E-5
8	1.0694997122400165	0.0	0.0	0.0	2.9615092640945627E-4
9	1.0762124665719641	0.0	0.0	0.0	2.9615092640945627E-4
10	1.0859854471434762	0.0	0.0	0.0	2.9615092640945627E-4
11	1.0960545786413978	0.0	0.0	0.0	2.9615092640945627E-4
12	1.115600539784422	0.0	0.0	0.0	2.9615092640945627E-4
13	1.1425502740876823	0.0	0.0	0.0	2.9615092640945627E-4
14	1.1756204608700718	0.0	0.0	0.0	2.9615092640945627E-4
15	1.222313590267296	0.0	0.0	0.0	2.9615092640945627E-4
16	1.2778912474568038	0.0	0.0	0.0	2.9615092640945627E-4
17	1.3575558466609476	0.0	0.0	0.0	2.9615092640945627E-4
18	1.4659470857268087	0.0	0.0	0.0	2.9615092640945627E-4
19	1.6299159819821776	0.0	0.0	0.0	2.9615092640945627E-4
20	1.8491663845006452	0.0	0.0	0.0	2.9615092640945627E-4
21	2.1477852352968467	0.0	0.0	0.0	2.9615092640945627E-4
22	2.5194546479407145	0.0	0.0	0.0	2.9615092640945627E-4
23	3.009485714172895	0.0	0.0	0.0	2.9615092640945627E-4
24	3.6099810759558024	0.0	0.0	0.0	2.9615092640945627E-4
25	4.30840367740477	0.0	0.0	0.0	2.9615092640945627E-4
26	5.139501893885174	0.0	0.0	0.0	2.9615092640945627E-4
27	6.130817761553095	0.0	0.0	0.0	2.9615092640945627E-4
28	7.19005090834425	0.0	0.0	0.0	2.9615092640945627E-4
29	8.357872728152206	0.0	0.0	0.0	2.9615092640945627E-4
30	9.698547972007814	0.0	0.0	0.0	2.9615092640945627E-4
31	11.061533252319602	0.0	0.0	0.0	2.9615092640945627E-4
32	12.58256441035857	0.0	0.0	0.0	3.9486790187927506E-4
33	14.19935103460326	0.0	0.0	0.0	3.9486790187927506E-4
34	15.841310487592757	0.0	0.0	0.0	3.9486790187927506E-4
35	17.572510086406968	0.0	0.0	0.0	3.9486790187927506E-4
36	19.46126197807101	0.0	0.0	0.0	3.9486790187927506E-4
37	21.30736813633209	0.0	0.0	0.0	3.9486790187927506E-4
38	23.256830967910073	0.0	0.0	0.0	3.9486790187927506E-4
39	25.28970964376005	0.0	0.0	0.0	3.9486790187927506E-4
40	27.38784024039558	0.0	0.0	0.0	3.9486790187927506E-4
41	29.52703709882655	0.0	0.0	0.0	4.935848773490938E-4
42	31.734447387307167	0.0	0.0	0.0	4.935848773490938E-4
43	33.8901299806416	0.0	0.0	0.0	5.923018528189125E-4
44	36.056079139424895	0.0	0.0	0.0	5.923018528189125E-4
45	38.23979735379275	0.0	0.0	0.0	8.884527792283689E-4
46	40.37524296715588	0.0	0.0	0.0	8.884527792283689E-4
47	42.515920580218896	0.0	0.0	0.0	8.884527792283689E-4
48	44.69914520970941	0.0	0.0	0.0	8.884527792283689E-4
49	46.76469920443989	0.0	0.0	0.0	8.884527792283689E-4
50	48.80814059666514	0.0	0.0	0.0	8.884527792283689E-4
>>END_MODULE