##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-23B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 614404 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.59233989362048 40.0 40.0 40.0 40.0 40.0 2 39.3707397738296 40.0 40.0 40.0 40.0 40.0 3 39.32157993763062 40.0 40.0 40.0 40.0 40.0 4 39.18661336840255 40.0 40.0 40.0 40.0 40.0 5 39.22453629859181 40.0 40.0 40.0 40.0 40.0 6 39.32620230337042 40.0 40.0 40.0 40.0 40.0 7 39.35838959381775 40.0 40.0 40.0 40.0 40.0 8 39.371547060240495 40.0 40.0 40.0 40.0 40.0 9 39.38286860111588 40.0 40.0 40.0 40.0 40.0 10-11 39.38776114738836 40.0 40.0 40.0 40.0 40.0 12-13 39.305378871231305 40.0 40.0 40.0 40.0 40.0 14-15 39.33974713706291 40.0 40.0 40.0 40.0 40.0 16-17 39.44638381260539 40.0 40.0 40.0 40.0 40.0 18-19 39.479036594813834 40.0 40.0 40.0 40.0 40.0 20-21 39.46026067538623 40.0 40.0 40.0 40.0 40.0 22-23 39.504544241248425 40.0 40.0 40.0 40.0 40.0 24-25 39.48095390003971 40.0 40.0 40.0 40.0 40.0 26-27 39.45301788399815 40.0 40.0 40.0 40.0 40.0 28-29 39.47786147225604 40.0 40.0 40.0 40.0 40.0 30-31 39.4557416032023 40.0 40.0 40.0 40.0 40.0 32-33 39.458498100153676 40.0 40.0 40.0 40.0 40.0 34-35 39.45884489652772 40.0 40.0 40.0 40.0 40.0 36-37 39.44469345020481 40.0 40.0 40.0 40.0 40.0 38-39 39.3822358583854 40.0 40.0 40.0 40.0 40.0 40-41 39.43282042003014 40.0 40.0 40.0 40.0 40.0 42-43 39.41071532772355 40.0 40.0 40.0 40.0 40.0 44-45 39.31900020137235 40.0 40.0 40.0 40.0 40.0 46-47 39.30696962503309 40.0 40.0 40.0 40.0 40.0 48-49 39.39113231170563 40.0 40.0 40.0 40.0 40.0 50-51 39.34038808545641 40.0 40.0 40.0 40.0 40.0 52-53 39.31021156325039 40.0 40.0 40.0 40.0 40.0 54-55 39.225583210208626 40.0 40.0 40.0 40.0 40.0 56-57 39.2446584458728 40.0 40.0 40.0 40.0 40.0 58-59 39.18827990595299 40.0 40.0 40.0 40.0 40.0 60-61 39.098552324399584 40.0 40.0 40.0 40.0 40.0 62-63 39.39099258040882 40.0 40.0 40.0 40.0 40.0 64-65 39.394949691930265 40.0 40.0 40.0 40.0 40.0 66-67 39.389429033606824 40.0 40.0 40.0 40.0 40.0 68-69 39.39388992925423 40.0 40.0 40.0 40.0 40.0 70-71 39.38515625278734 40.0 40.0 40.0 40.0 40.0 72-73 39.382511141886475 40.0 40.0 40.0 40.0 40.0 74-75 39.401273964027524 40.0 40.0 40.0 40.0 40.0 76-77 39.3776441991435 40.0 40.0 40.0 40.0 40.0 78-79 39.37693497740386 40.0 40.0 40.0 40.0 40.0 80-81 39.36794539111505 40.0 40.0 40.0 40.0 40.0 82-83 39.363274745770525 40.0 40.0 40.0 40.0 40.0 84-85 39.33163622244136 40.0 40.0 40.0 40.0 40.0 86-87 39.31797110485918 40.0 40.0 40.0 40.0 40.0 88-89 39.294737239586524 40.0 40.0 40.0 40.0 40.0 90-91 39.50813833095079 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0691600098664722 1101 2 -0.13150865826135316 1101 3 0.0013730471337680683 1101 4 0.02122974255379262 1101 5 -0.14548821518646804 1101 6 0.03729869228448024 1101 7 0.04125395582882874 1101 8 -0.024673908540840728 1101 9 -0.0839035107801891 1101 10-11 -0.003532431348155285 1101 12-13 -0.10705965126749106 1101 14-15 -0.04827201451438867 1101 16-17 -0.01323085620646225 1101 18-19 -0.027600700832557834 1101 20-21 0.03555851576858515 1101 22-23 -0.018668248441798596 1101 24-25 0.10061017750841472 1101 26-27 0.0958024069415302 1101 28-29 0.04153717964495485 1101 30-31 -0.03970405292180601 1101 32-33 -0.06522965011286885 1101 34-35 0.01977900347908701 1101 36-37 -0.04536561356646729 1101 38-39 0.012714755123425903 1101 40-41 -0.09695562747186415 1101 42-43 0.003534020573674468 1101 44-45 -0.04824250757941684 1101 46-47 -0.08633095597868845 1101 48-49 -0.056698388431684066 1101 50-51 -0.08533727995129681 1101 52-53 -0.10734915435780579 1101 54-55 0.07334140906669973 1101 56-57 -0.0065991659953894555 1101 58-59 0.18156620214490005 1101 60-61 0.02145237023455593 1101 62-63 -0.0032517382598840072 1101 64-65 -0.06776335527547417 1101 66-67 -0.139178012442855 1101 68-69 -0.20155675967907172 1101 70-71 -0.17643953936853052 1101 72-73 -0.30712559945293094 1101 74-75 -0.43513170461616113 1101 76-77 -0.03861837973686022 1101 78-79 0.20397147308014496 1101 80-81 0.3447122292736964 1101 82-83 0.4655617308789459 1101 84-85 0.4459958473719894 1101 86-87 0.5922384731932482 1101 88-89 0.11126556887859351 1101 90-91 0.42621039771774605 1102 1 0.04432497240985356 1102 2 -0.11616968730001531 1102 3 0.12953247535332935 1102 4 -0.2108093921912939 1102 5 -0.05065616124437611 1102 6 -0.04122071708279407 1102 7 0.0011498709863246859 1102 8 -0.13194413727829613 1102 9 -0.11189734947757302 1102 10-11 -0.11929341181148345 1102 12-13 -0.12291868528647854 1102 14-15 -0.16391742540934473 1102 16-17 -0.05098454495232829 1102 18-19 -0.03895974478329833 1102 20-21 -0.015484951029549165 1102 22-23 -0.07690303163148826 1102 24-25 -0.0634458809553422 1102 26-27 -0.028585119490493582 1102 28-29 -0.032358002368084726 1102 30-31 -0.11466175265401546 1102 32-33 0.02297314885044699 1102 34-35 -0.022062702344797458 1102 36-37 -0.10485042217487006 1102 38-39 0.02019685597454668 1102 40-41 -0.0475992754750294 1102 42-43 -0.08536805103145895 1102 44-45 -0.0033567676239698585 1102 46-47 -0.06317190045739807 1102 48-49 -0.14131637803887998 1102 50-51 -0.012406759696986569 1102 52-53 -0.07446955952840995 1102 54-55 -0.039228009169029576 1102 56-57 -0.032708609799087185 1102 58-59 -0.11373160817705497 1102 60-61 -0.1256904534002672 1102 62-63 -0.17686109528644778 1102 64-65 -0.15449377577390067 1102 66-67 -0.2327466329849628 1102 68-69 -0.3283621578699325 1102 70-71 -0.02871213545672191 1102 72-73 -0.47272104027072004 1102 74-75 -0.6281614713130779 1102 76-77 -0.2904510948048653 1102 78-79 -0.19764857115551848 1102 80-81 -0.15471360804687606 1102 82-83 -0.5527783637796091 1102 84-85 -0.537460136692637 1102 86-87 -0.19897031801554022 1102 88-89 -1.1982582406452167 1102 90-91 0.42621039771774605 1103 1 0.0646382702499082 1103 2 0.02700749701866556 1103 3 -3.1437506427067774E-4 1103 4 -0.012229837606980709 1103 5 0.26236316009837424 1103 6 -0.003117929396182717 1103 7 0.09336670137199832 1103 8 -0.012038262318917248 1103 9 0.059501635714518386 1103 10-11 0.060647527939202917 1103 12-13 0.07911735789106444 1103 14-15 -0.043647333676709366 1103 16-17 0.09784585500767662 1103 18-19 0.06296310553431539 1103 20-21 0.12451212095325559 1103 22-23 0.05735512664570308 1103 24-25 0.026914158037278924 1103 26-27 0.033079636823487135 1103 28-29 -5.775893748349858E-4 1103 30-31 -0.054355310659104816 1103 32-33 -0.024254718932887442 1103 34-35 -0.0063332622809042505 1103 36-37 -0.03870119370134972 1103 38-39 0.06573318844846199 1103 40-41 0.07854619488416148 1103 42-43 0.06435299058097854 1103 44-45 0.06399681219104991 1103 46-47 -0.0607919724122965 1103 48-49 0.008272728936582041 1103 50-51 -0.11667592960573359 1103 52-53 -0.07085486053388479 1103 54-55 -0.045858182553892846 1103 56-57 -0.17291524445681716 1103 58-59 -0.0028594794995839834 1103 60-61 0.23920725052182945 1103 62-63 -0.11579952289726947 1103 64-65 -0.066241632882587 1103 66-67 0.11740340409078698 1103 68-69 0.08085177052551273 1103 70-71 -0.25231203436946714 1103 72-73 0.019731189932066684 1103 74-75 0.10162689386162072 1103 76-77 -0.1302033470571189 1103 78-79 0.22607473065284722 1103 80-81 0.3407057822241484 1103 82-83 -0.2661129381156684 1103 84-85 0.19180229898488932 1103 86-87 0.7973666783214526 1103 88-89 0.24459890221192637 1103 90-91 0.42621039771774605 1104 1 0.047111417825071555 1104 2 -0.11847607894921452 1104 3 -0.0874304911655912 1104 4 -0.11997041822620957 1104 5 -0.029064409634862898 1104 6 0.009144390475917419 1104 7 0.102797522189924 1104 8 0.031060785939772018 1104 9 -0.12293645252378127 1104 10-11 -0.040031201110558357 1104 12-13 0.015338932900093027 1104 14-15 0.03393940122190742 1104 16-17 -0.13589432151397318 1104 18-19 -0.1696175784606737 1104 20-21 -0.09519094476750922 1104 22-23 -0.03198493413789549 1104 24-25 -0.0160229695892653 1104 26-27 -0.04769552652274456 1104 28-29 0.03593551317232624 1104 30-31 0.06304348366578694 1104 32-33 0.021704571961663532 1104 34-35 -0.005003184922379944 1104 36-37 0.014422497412773794 1104 38-39 -0.013512453846828976 1104 40-41 -0.0828490062625562 1104 42-43 -0.1122392902965359 1104 44-45 -0.06010553801202434 1104 46-47 -0.012401391187843558 1104 48-49 0.10976571832254933 1104 50-51 -0.015446231714065561 1104 52-53 0.007264213282780929 1104 54-55 0.11737896245762869 1104 56-57 0.044873743243968534 1104 58-59 -0.04362829835535109 1104 60-61 0.04542297367710546 1104 62-63 -0.009567674689968442 1104 64-65 0.01203356661550714 1104 66-67 0.05102675544596735 1104 68-69 -0.1380786153742406 1104 70-71 -0.2756126925495934 1104 72-73 -0.16262942386264 1104 74-75 0.07433244785116955 1104 76-77 0.05004055673484231 1104 78-79 0.08007395606355772 1104 80-81 -0.3878555273271047 1104 82-83 -0.3110470558718248 1104 84-85 0.02404110495503886 1104 86-87 -0.1629616390520141 1104 88-89 0.038538296151315876 1104 90-91 0.42621039771774605 1201 1 0.03418675080666134 1201 2 0.08925588882620161 1201 3 0.07568360621783654 1201 4 0.07505423634749775 1201 5 -0.03625148441931714 1201 6 0.088535443246613 1201 7 -0.05414016137377331 1201 8 0.040550302732064836 1201 9 0.1109362542477541 1201 10-11 -0.028862506093950913 1201 12-13 -0.06593013912595325 1201 14-15 0.036506507498664575 1201 16-17 -0.08359364507735734 1201 18-19 0.007599147635460213 1201 20-21 0.04389904211241458 1201 22-23 0.041117942440443755 1201 24-25 0.06186854754380278 1201 26-27 0.047529443248272685 1201 28-29 0.002663237748699032 1201 30-31 0.1283641336839736 1201 32-33 0.08034999899736306 1201 34-35 0.057302836070135754 1201 36-37 -0.006631501760125502 1201 38-39 0.10175149557231578 1201 40-41 0.0987071971837068 1201 42-43 -0.016436994690302242 1201 44-45 0.027223015776861814 1201 46-47 -0.03169769483454132 1201 48-49 0.1466091963418421 1201 50-51 -0.05275707900894844 1201 52-53 0.061395393987687896 1201 54-55 -0.01621577277612829 1201 56-57 -0.0565957735794953 1201 58-59 -0.042931784710312115 1201 60-61 -0.01096765328047411 1201 62-63 0.007613494782127361 1201 64-65 -0.23801253332916872 1201 66-67 -0.18727045312544277 1201 68-69 -0.13346352667393546 1201 70-71 -0.27883671769910023 1201 72-73 -0.2918439696610946 1201 74-75 -0.09187959964486936 1201 76-77 -0.07383855053023325 1201 78-79 -0.14486001865819276 1201 80-81 -0.5238707666777174 1201 82-83 -0.151503853975683 1201 84-85 -0.3216042944216966 1201 86-87 -0.3179215422299251 1201 88-89 -0.08267382506080168 1201 90-91 -0.3737896022822511 1202 1 -0.015301185773715531 1202 2 -0.06348873167618763 1202 3 0.04089450096809344 1202 4 -0.09768576654602867 1202 5 -0.028732333203983274 1202 6 -0.11949217954352775 1202 7 -0.12554670441192428 1202 8 -0.13498699548561177 1202 9 -0.1250696677854961 1202 10-11 -0.06698952107712586 1202 12-13 -0.1325560202556062 1202 14-15 -0.10784288578027912 1202 16-17 -0.1656233614953635 1202 18-19 -0.12912885214802827 1202 20-21 -0.058243000481184026 1202 22-23 -0.00806928155134301 1202 24-25 -0.07271169610690009 1202 26-27 -0.13540538464890517 1202 28-29 -0.035924869870321174 1202 30-31 -0.04672075915041063 1202 32-33 -0.042894895918117015 1202 34-35 -0.07985716948134325 1202 36-37 -0.27573060631920754 1202 38-39 -0.11304724673241395 1202 40-41 -0.19377845064366994 1202 42-43 -0.09941807756623433 1202 44-45 -0.21771788135411896 1202 46-47 -0.11252667184722043 1202 48-49 -0.07740031465377228 1202 50-51 -0.07991608187033705 1202 52-53 0.04474843967236808 1202 54-55 -0.06371169124190601 1202 56-57 -0.030903748975752876 1202 58-59 -0.30220382667147305 1202 60-61 0.27637398114892164 1202 62-63 -0.1224243611077398 1202 64-65 0.01842108714095758 1202 66-67 -0.08502121538118956 1202 68-69 -0.06932221362485791 1202 70-71 -0.16776113560464267 1202 72-73 -0.27614086774931224 1202 74-75 -0.11007936470393531 1202 76-77 -0.20695455478659142 1202 78-79 0.10937358022735566 1202 80-81 -0.014608088171158329 1202 82-83 0.07446932715905064 1202 84-85 -0.3219477010151053 1202 86-87 0.1007813124677952 1202 88-89 0.40930478456486696 1202 90-91 -0.573789602282254 1203 1 -0.03863311491353727 1203 2 0.18786875665801261 1203 3 -0.027327428778257 1203 4 0.08357252562543493 1203 5 -0.03180675468675531 1203 6 0.035772071225331104 1203 7 0.025080061946958665 1203 8 0.08239541767009939 1203 9 0.048330885650145206 1203 10-11 0.038432378309479986 1203 12-13 -0.007005874141036372 1203 14-15 0.026722381542924722 1203 16-17 -0.009517920372559274 1203 18-19 -0.07102374445212689 1203 20-21 0.02033306112390676 1203 22-23 -0.018367068902527706 1203 24-25 -0.14578853377473422 1203 26-27 0.014754716612124241 1203 28-29 -0.1378905308759144 1203 30-31 -0.027859269527581887 1203 32-33 0.05820006618726836 1203 34-35 -0.04962240994068168 1203 36-37 -0.07544706704144488 1203 38-39 0.005888598153433122 1203 40-41 -0.05297002897290071 1203 42-43 0.06662577059345409 1203 44-45 -0.0017187054395435553 1203 46-47 0.01608319446585682 1203 48-49 0.16012134988491766 1203 50-51 0.05227520772023553 1203 52-53 0.009921366822645439 1203 54-55 -0.03272513896621376 1203 56-57 0.15821452726164154 1203 58-59 0.05475003395687139 1203 60-61 -0.021496273596333992 1203 62-63 0.008535191138186349 1203 64-65 0.19356399967291793 1203 66-67 -0.15370355815573333 1203 68-69 0.21575804964250267 1203 70-71 0.11028814513483098 1203 72-73 0.18491626963940888 1203 74-75 -0.005206586793846668 1203 76-77 -0.15307131242508376 1203 78-79 0.017442275235467264 1203 80-81 -0.016108572731312165 1203 82-83 0.2582699016804213 1203 84-85 -0.46885704167444686 1203 86-87 0.46460510027685586 1203 88-89 0.644598902211925 1203 90-91 0.42621039771774605 1204 1 -0.09406699923847128 1204 2 0.030794366823393204 1204 3 -0.2574041148165165 1204 4 -0.28991806206208537 1204 5 -0.1493044631259295 1204 6 -0.12456688079847567 1204 7 -0.09135510512142275 1204 8 -0.315591988362641 1204 9 -0.06775806007269125 1204 10-11 -0.19556483334849162 1204 12-13 -0.06143757368988645 1204 14-15 -0.19490106493801562 1204 16-17 0.08338215431044205 1204 18-19 -0.16645282677984596 1204 20-21 -0.1290282865128347 1204 22-23 -0.17926382198303514 1204 24-25 -0.10307927878735512 1204 26-27 0.01893722964848621 1204 28-29 -0.0570332549879069 1204 30-31 -0.11058030399981078 1204 32-33 -0.09020888235144753 1204 34-35 -0.08942948843825604 1204 36-37 -0.0388491279130605 1204 38-39 -0.10181232864314893 1204 40-41 -0.12414876416345777 1204 42-43 -0.12562017622656185 1204 44-45 0.04057357756314417 1204 46-47 0.11986486329036694 1204 48-49 -0.07566666355106122 1204 50-51 0.06279909772147363 1204 52-53 0.09649761411149882 1204 54-55 0.1533539771524559 1204 56-57 0.13086530057372414 1204 58-59 0.050516699183106084 1204 60-61 0.20038312600314612 1204 62-63 -0.05237588131361548 1204 64-65 -0.13284635483907437 1204 66-67 -0.027605721883134038 1204 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0.02739252107853929 2401 34-35 0.006802238420007711 2401 36-37 0.09262773825371795 2401 38-39 0.11788378860656223 2401 40-41 0.05996621459333795 2401 42-43 0.1584235117244006 2401 44-45 0.07393880027433397 2401 46-47 0.03217300957282987 2401 48-49 0.12221178499196128 2401 50-51 0.13016015551045967 2401 52-53 0.07175615485613918 2401 54-55 0.020335357777149454 2401 56-57 0.09358701599321506 2401 58-59 0.04256169979640134 2401 60-61 0.06712499271731787 2401 62-63 0.12271392140483073 2401 64-65 0.13740993903765286 2401 66-67 0.187688883948276 2401 68-69 0.14316555553583754 2401 70-71 0.2368092882687236 2401 72-73 0.09470405066875998 2401 74-75 0.013276745860494543 2401 76-77 0.1425039894660074 2401 78-79 0.21611216730291005 2401 80-81 0.34831155015655213 2401 82-83 -0.5542489520149019 2401 84-85 6.1585830692934E-4 2401 86-87 -0.9942999883452188 2401 88-89 -1.5347489238750285 2401 90-91 -0.573789602282254 2402 1 0.013763423039101497 2402 2 -0.02938411959428322 2402 3 0.017507053449691057 2402 4 0.016895443903258922 2402 5 0.2067975035164622 2402 6 0.045623366513652286 2402 7 0.05198717649209783 2402 8 0.09189539914581246 2402 9 0.0898004240740562 2402 10-11 0.11304718318134377 2402 12-13 0.04888722075488516 2402 14-15 0.031907628331815374 2402 16-17 -6.86885994326758E-4 2402 18-19 0.10913909830877344 2402 20-21 0.04029369635938451 2402 22-23 0.006512996033528395 2402 24-25 0.05032851590430454 2402 26-27 0.02446766265565259 2402 28-29 -0.006648395603001234 2402 30-31 0.04199829033890978 2402 32-33 0.04044935856448717 2402 34-35 0.008833397067732562 2402 36-37 0.09339985700916742 2402 38-39 -0.401195071031097 2402 40-41 -0.03402929978852143 2402 42-43 0.010872594450610507 2402 44-45 0.09406905113331732 2402 46-47 0.09889009526334291 2402 48-49 -0.02280709926633051 2402 50-51 0.11072268850673339 2402 52-53 0.10736182621609203 2402 54-55 -0.09258578391328598 2402 56-57 0.11954263597036174 2402 58-59 0.024768139017211865 2402 60-61 0.08348223171982738 2402 62-63 0.17352245671354893 2402 64-65 0.18577775193168833 2402 66-67 0.08462858605778933 2402 68-69 0.26224000427409067 2402 70-71 0.21772300443817727 2402 72-73 0.07511228139387782 2402 74-75 0.09559794338822769 2402 76-77 0.11182724163083435 2402 78-79 0.26159728704163854 2402 80-81 0.025706966363003403 2402 82-83 0.0556916156796774 2402 84-85 0.32300823590511385 2402 86-87 -0.3074689116205178 2402 88-89 0.11126556887859351 2402 90-91 0.42621039771774605 2403 1 -0.011412489344671428 2403 2 0.02210922924562908 2403 3 0.13729416609660916 2403 4 0.06912604391836652 2403 5 0.12325641320704506 2403 6 0.10051455208848381 2403 7 0.1750343976324089 2403 8 0.08372730425753616 2403 9 0.1747641661017667 2403 10-11 0.14947468273118858 2403 12-13 0.05356349963756202 2403 14-15 0.0985668819784209 2403 16-17 0.05390453811367024 2403 18-19 0.06785870366064728 2403 20-21 0.047934373099252525 2403 22-23 -0.014616946714056667 2403 24-25 0.13417949475714153 2403 26-27 0.11121539049412377 2403 28-29 0.07206937067176966 2403 30-31 -0.016753336829118837 2403 32-33 0.03863936268630397 2403 34-35 0.0953933636574078 2403 36-37 0.09284873391590764 2403 38-39 0.19349183798586012 2403 40-41 0.04720795827444846 2403 42-43 0.09084826747758967 2403 44-45 0.13190353889505957 2403 46-47 0.11178249624838799 2403 48-49 0.11609007959071249 2403 50-51 0.08619834257311965 2403 52-53 0.06014233039240224 2403 54-55 0.026582186160126753 2403 56-57 -0.09977352032714748 2403 58-59 0.030254807788672622 2403 60-61 -0.04672145491907287 2403 62-63 0.07848692634744481 2403 64-65 -0.09397265190575865 2403 66-67 0.2319153900756774 2403 68-69 0.1946106693123255 2403 70-71 -0.013601790565154204 2403 72-73 0.11062438203018843 2403 74-75 0.02658577689178543 2403 76-77 -0.012720529187703278 2403 78-79 -0.08954284331090179 2403 80-81 0.027862963251500616 2403 82-83 -0.3996493551668152 2403 84-85 -0.21862323292999974 2403 86-87 -0.18890783148246726 2403 88-89 -1.3575691194682946 2403 90-91 -1.2774933059859634 2404 1 0.020856175047448744 2404 2 0.019498927224212537 2404 3 -0.06584931680986728 2404 4 0.1208896738841716 2404 5 0.0011238261636847824 2404 6 0.013654363630458022 2404 7 1.554031165724723E-4 2404 8 0.03092950972587971 2404 9 0.07658116032413886 2404 10-11 0.0505742654321395 2404 12-13 -0.0294193746213125 2404 14-15 0.08237714810918817 2404 16-17 0.018602431384749707 2404 18-19 0.13333986819579025 2404 20-21 9.694112451086312E-4 2404 22-23 0.054741532020230466 2404 24-25 -0.11173126960729007 2404 26-27 -3.9850641340422044E-4 2404 28-29 -0.04839675010106248 2404 30-31 0.09310256060873456 2404 32-33 -0.003940732631178889 2404 34-35 -0.039008879674568675 2404 36-37 0.07922560466764139 2404 38-39 0.07231374201154495 2404 40-41 0.034553862939539215 2404 42-43 -0.008490486299173483 2404 44-45 0.11771142123994593 2404 46-47 0.06970347915525821 2404 48-49 0.06826530205680115 2404 50-51 0.00904304350615348 2404 52-53 0.02090331170761317 2404 54-55 0.2139795569027214 2404 56-57 -0.038233218848098716 2404 58-59 0.0045258989220968715 2404 60-61 -0.03901563853837331 2404 62-63 -0.2289153128924042 2404 64-65 -0.20725268585498924 2404 66-67 -0.0724801109069162 2404 68-69 0.051432025108937296 2404 70-71 -0.09310960512153343 2404 72-73 -0.019113790673202402 2404 74-75 0.11744049202816598 2404 76-77 0.026715329504412466 2404 78-79 -0.5477808872002328 2404 80-81 -0.49181042063464986 2404 82-83 0.161173677694201 2404 84-85 0.26502263796794523 2404 86-87 0.4189882999430736 2404 88-89 0.05200630961932973 2404 90-91 0.42621039771774605 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 16.0 31 407.0 32 2627.0 33 4682.0 34 6396.0 35 7912.0 36 10981.0 37 16367.0 38 40814.0 39 240154.0 40 284048.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.544762078371885 10.499931641070045 34.735939219145706 30.21936706141236 2 26.97278679024765 16.76205451757513 30.080379728291124 26.184778963886096 3 28.65345928737443 18.899779298311863 21.67629116997936 30.770470244334348 4 26.798653654598603 20.000195311228442 20.797878920059116 32.40327211411384 5 27.384261821212103 20.206248657235303 21.242537483479925 31.166952038072672 6 28.753881810665295 22.201841133846784 23.060885020279816 25.9833920352081 7 26.398916673719576 21.67987187583414 22.83285916107317 29.088352289373116 8 25.662593342491256 22.53907852162421 23.01124341638401 28.787084719500523 9 26.24071457868113 22.756199503909478 23.09034446390323 27.91274145350616 10-11 25.53450172850437 21.70851752267238 23.08953066711805 29.667450081705198 12-13 26.20881374470218 21.53648088228592 22.792006562457274 29.462698810554617 14-15 26.493235721121604 21.27240382549593 22.70452340805073 29.529837045331735 16-17 26.37686278084127 20.991155656538695 22.425391110734957 30.20659045188508 18-19 26.7472216977754 21.3076412262941 22.326026523264822 29.619110552665674 20-21 26.70392770880398 21.400495439482818 22.369727410628837 29.525849441084368 22-23 26.245353220356638 21.058863549065435 22.636327237452882 30.059455993125045 24-25 26.529693817097545 21.322289568427287 22.611750574540533 29.536266039934635 26-27 26.692615933490018 21.472435075292477 22.608332628042785 29.226616363174717 28-29 26.438141678765113 21.173690275453936 22.833672957858347 29.554495087922604 30-31 26.855969114382255 21.356340210548247 22.84796776020677 28.939722914862724 32-33 26.81533416640808 21.385895317728558 22.88962318743343 28.909147328429935 34-35 26.412887055854256 21.037931013314836 23.221202711199705 29.327979219631207 36-37 26.62713749427292 21.38094692658991 23.22358892123971 28.768326657897457 38-39 26.89576477041216 21.237070992007524 23.12793841090738 28.739225826672936 40-41 26.539089853183256 21.059783744035833 23.387376434116298 29.01374996866461 42-43 26.755679504423906 21.242709559157603 23.53167520560193 28.469935730816566 44-45 26.826793618814232 21.238655185439892 23.46536259819258 28.4691885975533 46-47 26.384962457400686 20.954855566464133 23.73273191574371 28.927450060391475 48-49 26.72158301596491 21.155053448625104 23.656745042659775 28.466618492750207 50-51 26.783283301442275 21.21357439082295 23.64476147959787 28.3583808281369 52-53 26.61782129913577 20.965639810426538 23.7477348759409 28.668804014496796 54-55 26.629631349602555 21.369997136245075 23.74130231190161 28.259069202250757 56-57 26.961154368785458 21.136250889718095 23.6112848607726 28.29130988072385 58-59 26.52247558892253 21.015394503792447 23.897363531143 28.56476637614202 60-61 27.112424136083067 21.34309396924366 23.616767183806267 27.927714710867 62-63 26.955406313790064 21.304570374892847 23.629843419486534 28.110179891830555 64-65 26.724741003223617 21.052298541462335 23.874458189641533 28.348502265672515 66-67 26.8479852814796 21.3826048714752 23.78283726916575 27.986572577879453 68-69 27.119861225098855 21.115608445870325 23.667275982988883 28.097254346041932 70-71 26.81346376331412 21.122813116143156 23.87715972603186 28.18656339451086 72-73 27.08947728683014 21.33713752007114 23.864021648040225 27.7093635450585 74-75 27.103230470682245 21.143031087097143 23.729690651040674 28.02404779117994 76-77 27.100427703434477 20.869357710593462 23.7687464434532 28.261468142518858 78-79 27.29552534214995 21.56472453695894 23.39777110533833 27.741979015552783 80-81 27.294594252437015 21.17286295030313 23.624650096355378 27.907892700904473 82-83 27.4826537487935 20.578754712170603 23.76208774198248 28.176503797053414 84-85 28.05215329909127 20.295831687080206 23.244271039114974 28.40774397471355 86-87 28.63750448189315 20.02868411617067 22.753675152384368 28.580136249551813 88-89 28.04827050600394 20.01314295955553 23.388493936316387 28.550092598124145 90-91 25.100611119391864 20.032791772246235 26.53152481740945 28.33507229095245 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 2.5 10 3.0 11 7.5 12 13.5 13 18.0 14 26.0 15 38.0 16 53.5 17 74.5 18 99.5 19 131.5 20 195.0 21 280.5 22 355.0 23 475.5 24 688.0 25 898.0 26 1128.5 27 1464.0 28 1870.5 29 2352.0 30 2912.5 31 3467.0 32 4063.0 33 4743.5 34 5678.0 35 6788.0 36 8001.0 37 9222.0 38 10586.5 39 12238.0 40 13762.0 41 15442.5 42 17385.0 43 19396.5 44 21647.5 45 24172.0 46 26784.5 47 29333.0 48 31603.5 49 34139.5 50 37171.0 51 39835.5 52 41788.0 53 43502.5 54 45186.0 55 46609.0 56 47664.0 57 48081.5 58 47976.0 59 47368.0 60 46545.0 61 45558.0 62 44212.0 63 42595.5 64 40258.5 65 37405.5 66 34376.5 67 31554.5 68 28541.5 69 25013.0 70 21716.5 71 18800.0 72 15893.5 73 13119.0 74 10628.0 75 8537.0 76 6793.0 77 5182.5 78 4030.5 79 3101.0 80 2234.5 81 1612.5 82 1187.5 83 861.0 84 625.5 85 446.5 86 316.5 87 232.5 88 168.5 89 119.0 90 78.5 91 51.5 92 36.5 93 25.0 94 18.5 95 13.5 96 7.0 97 4.0 98 3.5 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011393154992480517 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.055602979066653097 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 4.6631133742754686E-4 70-71 0.0 72-73 0.0012794186321735403 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0014066280312834073 82-83 0.001821095571095571 84-85 0.0 86-87 0.0 88-89 0.005973715651135005 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 11927.0 32-33 25493.0 34-35 28404.0 36-37 29796.0 38-39 31909.0 40-41 33030.0 42-43 32652.0 44-45 32663.0 46-47 32663.0 48-49 31147.0 50-51 30434.0 52-53 29060.0 54-55 26900.0 56-57 25395.0 58-59 23574.0 60-61 21902.0 62-63 20360.0 64-65 18868.0 66-67 17047.0 68-69 15474.0 70-71 14352.0 72-73 12712.0 74-75 11490.0 76-77 10374.0 78-79 9080.0 80-81 8353.0 82-83 7426.0 84-85 6503.0 86-87 5710.0 88-89 5195.0 90-91 4511.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.92421653797177 #Duplication Level Percentage of deduplicated Percentage of total 1 99.92863560858936 99.8529062289676 2 0.06808172790786986 0.13606026643490526 3 0.0023655018132779544 0.007091127462328535 4 6.404413765083154E-4 0.0025598241114437445 5 2.767203129783134E-4 0.0013825530237250153 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0016275935703543598 0.0 0.0 0.0 0.0 2 0.0017903529273897956 0.0 0.0 0.0 0.0 3 0.0019531122844252315 0.0 0.0 0.0 0.0 4 0.0019531122844252315 0.0 0.0 0.0 0.0 5 0.0019531122844252315 0.0 0.0 0.0 1.6275935703543597E-4 6 0.0019531122844252315 0.0 0.0 0.0 1.6275935703543597E-4 7 0.0022786309984961037 0.0 0.0 0.0 1.6275935703543597E-4 8 0.006673133638452874 0.0 0.0 0.0 3.2551871407087194E-4 9 0.00683589299548831 0.0 0.0 0.0 3.2551871407087194E-4 10-11 0.00683589299548831 0.0 0.0 0.0 3.2551871407087194E-4 12-13 0.00683589299548831 0.0 0.0 0.0 3.2551871407087194E-4 14-15 0.00683589299548831 0.0 0.0 0.0 3.2551871407087194E-4 16-17 0.00683589299548831 0.0 0.0 0.0 3.2551871407087194E-4 18-19 0.006917272674006028 0.0 0.0 0.0 3.2551871407087194E-4 20-21 0.0069986523525237465 0.0 0.0 0.0 3.2551871407087194E-4 22-23 0.0070800320310414645 0.0 0.0 0.0 3.2551871407087194E-4 24-25 0.007242791388076901 0.0 0.0 0.0 3.2551871407087194E-4 26-27 0.007405550745112337 0.0 0.0 0.0 3.2551871407087194E-4 28-29 0.00764968978066549 0.0 0.0 0.0 3.2551871407087194E-4 30-31 0.007812449137700926 0.0 0.0 0.0 3.2551871407087194E-4 32-33 0.007975208494736362 0.0 0.0 0.0 4.882780711063079E-4 34-35 0.008382106887324953 0.0 0.0 0.0 4.882780711063079E-4 36-37 0.00887038495843126 0.0 0.0 0.0 4.882780711063079E-4 38-39 0.009684181743608439 0.0 0.0 0.0 4.882780711063079E-4 40-41 0.01009108013619703 0.0 0.0 0.0 5.696577496240259E-4 42-43 0.010172459814714749 0.0 0.0 0.0 7.324171066594618E-4 44-45 0.010416598850267902 0.0 0.0 0.0 8.951764636948978E-4 46-47 0.010579358207303338 0.0 0.0 0.0 9.765561422126158E-4 48-49 0.010579358207303338 0.0 0.0 0.0 9.765561422126158E-4 50-51 0.010823497242856493 0.0 0.0 0.0 9.765561422126158E-4 52-53 0.011067636278409647 0.0 0.0 0.0 9.765561422126158E-4 54-55 0.011149015956927364 0.0 0.0 0.0 9.765561422126158E-4 56-57 0.011230395635445081 1.6275935703543597E-4 0.0 0.0 9.765561422126158E-4 58-59 0.011230395635445081 1.6275935703543597E-4 0.0 0.0 9.765561422126158E-4 60-61 0.011230395635445081 1.6275935703543597E-4 0.0 0.0 9.765561422126158E-4 62-63 0.011230395635445081 1.6275935703543597E-4 0.0 0.0 9.765561422126158E-4 64-65 0.011393154992480517 1.6275935703543597E-4 0.0 0.0 9.765561422126158E-4 66-67 0.011555914349515953 1.6275935703543597E-4 0.0 0.0 9.765561422126158E-4 68-69 0.011555914349515953 1.6275935703543597E-4 0.0 0.0 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