##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-23A_S7_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	992685
Sequences flagged as poor quality	0
Sequence length	61
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.467871479875285	40.0	40.0	40.0	40.0	40.0
2	39.24075411636118	40.0	40.0	40.0	40.0	40.0
3	39.17035514790694	40.0	40.0	40.0	40.0	40.0
4	39.01962455360965	40.0	40.0	40.0	40.0	40.0
5	39.102712340772754	40.0	40.0	40.0	40.0	40.0
6	39.20669295899505	40.0	40.0	40.0	40.0	40.0
7	39.2049965497615	40.0	40.0	40.0	40.0	40.0
8	39.21998216957041	40.0	40.0	40.0	40.0	40.0
9	39.243010622705086	40.0	40.0	40.0	40.0	40.0
10	39.24817641044238	40.0	40.0	40.0	40.0	40.0
11	39.24494074152425	40.0	40.0	40.0	40.0	40.0
12	39.25567929403587	40.0	40.0	40.0	40.0	40.0
13	39.070855306567545	40.0	40.0	40.0	40.0	40.0
14	39.247451104831846	40.0	40.0	40.0	40.0	40.0
15	39.16477432418139	40.0	40.0	40.0	40.0	40.0
16	39.338291603076506	40.0	40.0	40.0	40.0	40.0
17	39.28556591466578	40.0	40.0	40.0	40.0	40.0
18	39.3581569178541	40.0	40.0	40.0	40.0	40.0
19	39.31973183839788	40.0	40.0	40.0	40.0	40.0
20	39.32908828077386	40.0	40.0	40.0	40.0	40.0
21	39.31122964485209	40.0	40.0	40.0	40.0	40.0
22	39.37324730402897	40.0	40.0	40.0	40.0	40.0
23	39.3636934173479	40.0	40.0	40.0	40.0	40.0
24	39.35945843847746	40.0	40.0	40.0	40.0	40.0
25	39.31876879372611	40.0	40.0	40.0	40.0	40.0
26	39.287951364229336	40.0	40.0	40.0	40.0	40.0
27	39.30893687322766	40.0	40.0	40.0	40.0	40.0
28	39.316995824455894	40.0	40.0	40.0	40.0	40.0
29	39.32502656935483	40.0	40.0	40.0	40.0	40.0
30	39.31407848411127	40.0	40.0	40.0	40.0	40.0
31	39.271793167016725	40.0	40.0	40.0	40.0	40.0
32	39.300946423084866	40.0	40.0	40.0	40.0	40.0
33	39.29827891022832	40.0	40.0	40.0	40.0	40.0
34	39.291598039660116	40.0	40.0	40.0	40.0	40.0
35	39.30123654532908	40.0	40.0	40.0	40.0	40.0
36	39.27088653500355	40.0	40.0	40.0	40.0	40.0
37	39.27738607916912	40.0	40.0	40.0	40.0	40.0
38	39.264995441655714	40.0	40.0	40.0	40.0	40.0
39	38.03766149382735	40.0	40.0	40.0	40.0	40.0
40	39.26752998181699	40.0	40.0	40.0	40.0	40.0
41	39.28287422495555	40.0	40.0	40.0	40.0	40.0
42	39.24803940827151	40.0	40.0	40.0	40.0	40.0
43	39.26379868739832	40.0	40.0	40.0	40.0	40.0
44	39.235507739111604	40.0	40.0	40.0	40.0	40.0
45	39.10333288001733	40.0	40.0	40.0	40.0	40.0
46	39.098328271304595	40.0	40.0	40.0	40.0	40.0
47	39.1927509733702	40.0	40.0	40.0	40.0	40.0
48	39.2736104605187	40.0	40.0	40.0	40.0	40.0
49	39.26259790366531	40.0	40.0	40.0	40.0	40.0
50	39.26002306874789	40.0	40.0	40.0	40.0	40.0
51	39.22337498803749	40.0	40.0	40.0	40.0	40.0
52	39.23917053244483	40.0	40.0	40.0	40.0	40.0
53	39.17341754937367	40.0	40.0	40.0	40.0	40.0
54	39.12354875917335	40.0	40.0	40.0	40.0	40.0
55	39.16080126122587	40.0	40.0	40.0	40.0	40.0
56	39.15809345361318	40.0	40.0	40.0	40.0	40.0
57	39.099375934964264	40.0	40.0	40.0	40.0	40.0
58	39.0789505230763	40.0	40.0	40.0	40.0	40.0
59	39.043954527367696	40.0	40.0	40.0	40.0	40.0
60	39.00729838770607	40.0	40.0	40.0	40.0	40.0
61	38.89143887537335	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.019852256282490544
1101	2	-0.07286842036486973
1101	3	0.06818187460158498
1101	4	-0.025752981726370194
1101	5	0.07307775764724056
1101	6	-0.05079039099617688
1101	7	-0.05568022098893266
1101	8	0.04706268407485226
1101	9	0.10127854038617556
1101	10	0.033502462869520855
1101	11	0.004836886960717379
1101	12	-0.002713745468483353
1101	13	0.12295655444128073
1101	14	-0.01849656151274104
1101	15	0.059115993792126176
1101	16	0.049890352787294034
1101	17	0.04915937040273377
1101	18	0.02813186832554493
1101	19	0.08118367885765565
1101	20	0.10541924496853028
1101	21	0.017486496485304315
1101	22	0.057780610631382956
1101	23	-0.018670139700859067
1101	24	0.04780328100348186
1101	25	0.13273890032260027
1101	26	0.038634367032585715
1101	27	0.08233696253107325
1101	28	0.15879755061169476
1101	29	0.14813113811165834
1101	30	0.16379210308279113
1101	31	0.18486573845440546
1101	32	0.08902530506225759
1101	33	0.0714391439356632
1101	34	0.027145792338600927
1101	35	0.10108934368705036
1101	36	-0.06840355735452164
1101	37	0.039036073011907035
1101	38	-0.02075146163303998
1101	39	1.1656714978048512
1101	40	0.018356059320019824
1101	41	0.033596970407963056
1101	42	0.05941449668507914
1101	43	0.12309371452825246
1101	44	0.1711721385855327
1101	45	0.031159304032037483
1101	46	0.14406508887811498
1101	47	-0.00965220699288949
1101	48	0.104220512905421
1101	49	0.05138068070152002
1101	50	0.06027125453350379
1101	51	0.0620522425255885
1101	52	0.1593644470433162
1101	53	0.041427193101483795
1101	54	0.06500584755735161
1101	55	0.0484687872656977
1101	56	0.12227512727634604
1101	57	0.1757119488008243
1101	58	0.030330093191587082
1101	59	0.03281060954417825
1101	60	0.23896410871714124
1101	61	0.09741817612590609
1102	1	0.1502408978535712
1102	2	0.06488592496574341
1102	3	0.054236182345469786
1102	4	0.06454049951582164
1102	5	0.020734682240131974
1102	6	0.004021218223748235
1102	7	0.010569944087919225
1102	8	0.06503523560422764
1102	9	0.06580682693927287
1102	10	-0.03908462151776604
1102	11	-0.04226639527497866
1102	12	-0.12127041508114189
1102	13	-0.05055191273127946
1102	14	-0.042702414883862616
1102	15	-0.008485261807791744
1102	16	-0.030191728501627324
1102	17	0.06341591506490829
1102	18	-0.01617983740084128
1102	19	0.004955761060848829
1102	20	-0.04859694780059698
1102	21	-0.014200131781947789
1102	22	0.009700073445920054
1102	23	-0.06307733603564003
1102	24	-0.048833214694354865
1102	25	0.027262412345272935
1102	26	0.04010126531476743
1102	27	-0.010504289411755963
1102	28	0.004870753305745268
1102	29	-0.02114729828041817
1102	30	-0.07815062286932317
1102	31	0.0104734752253961
1102	32	0.02299659692828726
1102	33	0.0921382570920386
1102	34	-0.05813747953534687
1102	35	0.014647994221895999
1102	36	-0.09136396938633595
1102	37	-0.027122664886690018
1102	38	-0.005361219960533958
1102	39	1.246064431499832
1102	40	0.015950508674443142
1102	41	0.019758959050676594
1102	42	0.0774479996666173
1102	43	-0.0029396665503895747
1102	44	0.06305238929917323
1102	45	-0.040422081394474674
1102	46	0.14921345487126558
1102	47	-0.024993687503666706
1102	48	-0.017993243837331363
1102	49	0.03368850585211902
1102	50	0.07963146745724003
1102	51	-0.0564739513609851
1102	52	-0.039384016472162386
1102	53	0.04652273450275857
1102	54	0.042029181804956295
1102	55	0.12246588191314345
1102	56	-0.15572444606348768
1102	57	0.08779151076305425
1102	58	-0.015734982642179318
1102	59	0.10660656439947047
1102	60	0.07953949042560993
1102	61	0.11622982598013465
1103	1	0.08559117103499858
1103	2	0.149851848010087
1103	3	0.06812324900541
1103	4	-0.0036519462110646828
1103	5	-0.04229782275634619
1103	6	-0.00897649876549167
1103	7	-0.04336721730243909
1103	8	-0.09101443708267709
1103	9	-0.04415080050334552
1103	10	0.09236052582282639
1103	11	-0.05526900552238345
1103	12	0.13660062004174733
1103	13	0.09589107087171556
1103	14	0.027349468944073863
1103	15	0.025672126782659177
1103	16	0.0720296577889954
1103	17	0.07623747476036158
1103	18	0.06698842492672696
1103	19	-0.012949893863527961
1103	20	-0.028249557054053298
1103	21	-0.01681924821243541
1103	22	0.032848854489436974
1103	23	0.04682157230904238
1103	24	-0.02330631018476481
1103	25	0.0464904702875657
1103	26	0.055418386672158704
1103	27	-0.031030284081161597
1103	28	0.011263795092638418
1103	29	-0.04807000468034062
1103	30	0.07511233929508876
1103	31	-0.004965977586003589
1103	32	0.03961654827094918
1103	33	0.03345952854400025
1103	34	0.11298832154453464
1103	35	0.11432488626897452
1103	36	0.07197421133086124
1103	37	0.18620871114337945
1103	38	-0.00932720569974066
1103	39	1.2625938575665216
1103	40	0.02097955474905433
1103	41	-0.03909103353297638
1103	42	-0.06702300089763469
1103	43	0.09405816665373123
1103	44	0.02847418050691175
1103	45	0.05234920007337962
1103	46	0.037803753069475476
1103	47	0.046272947662650665
1103	48	0.057269959931694814
1103	49	0.031047369759910737
1103	50	-0.009806281888664614
1103	51	0.12473196152984656
1103	52	0.06388085712121949
1103	53	0.05138051654174802
1103	54	0.03404705443038836
1103	55	0.06098331643905652
1103	56	0.041813939253067645
1103	57	-0.003254897203170515
1103	58	0.03873187394407296
1103	59	0.15098596928752528
1103	60	0.06115918945964438
1103	61	0.11178185431209897
1104	1	-0.022839573371257416
1104	2	0.041857182812655935
1104	3	-0.034795053208910076
1104	4	0.015441305768938207
1104	5	0.03647712949548776
1104	6	0.02950754153744839
1104	7	-0.02138441426147608
1104	8	-0.0310119429100979
1104	9	0.04568151651481145
1104	10	0.020384800297293282
1104	11	0.0886575840013748
1104	12	0.05979614432524727
1104	13	0.08466007640661388
1104	14	-0.023107802711820113
1104	15	0.07847841177676429
1104	16	-0.07139248332583747
1104	17	0.03276508930083111
1104	18	0.05699168499474183
1104	19	-0.05665966680961532
1104	20	-0.026553359303861157
1104	21	-0.04649067906952098
1104	22	-0.024329851371831523
1104	23	-0.036587648831435615
1104	24	-0.08256937441612422
1104	25	0.07929485457780316
1104	26	0.050131302195495664
1104	27	0.08025111512559846
1104	28	0.041037479005588295
1104	29	0.019462324327484737
1104	30	0.00817989488164983
1104	31	0.09644339031768112
1104	32	0.06418214320529358
1104	33	0.05194086615833271
1104	34	-0.026717021237786298
1104	35	0.0636799501811467
1104	36	-0.07900062118464746
1104	37	-0.04104128715240307
1104	38	-0.02576548715870075
1104	39	1.2014601639014373
1104	40	-0.0826485039706597
1104	41	0.03499872379846636
1104	42	0.018405617848038958
1104	43	-0.08208174439813831
1104	44	-0.02946400491437373
1104	45	-0.09233085412474651
1104	46	0.09420774166044055
1104	47	0.04910475582148166
1104	48	0.023337584087961716
1104	49	0.020409443018806428
1104	50	0.04011285799872866
1104	51	0.0025680302326733795
1104	52	0.059469139001215865
1104	53	-0.014204994480934374
1104	54	-0.029503019292057786
1104	55	0.056807381836101456
1104	56	0.024996319006483247
1104	57	0.07246649810593908
1104	58	0.0812631302972946
1104	59	0.022972762202634556
1104	60	-0.023130209946003788
1104	61	0.10502312781576251
1201	1	0.10807597826800475
1201	2	0.03123848227852477
1201	3	0.07421743318248275
1201	4	0.028936569496465836
1201	5	0.08938705571372196
1201	6	0.055867523786403694
1201	7	0.007756352162232361
1201	8	-0.1504803055039261
1201	9	-0.05821158132984294
1201	10	0.007690887692454851
1201	11	-0.018402606726581894
1201	12	0.044022936270870616
1201	13	0.08579346146061795
1201	14	-0.02132471664798885
1201	15	-0.06711048551939314
1201	16	-0.07146910654075356
1201	17	0.010560320144229252
1201	18	0.0734034198401119
1201	19	0.032322234148495
1201	20	-0.11014290151207717
1201	21	0.06159275084098681
1201	22	-0.025493566448403726
1201	23	-0.06365320318442969
1201	24	0.010387355815886679
1201	25	-0.023070567919411644
1201	26	0.043316625018675836
1201	27	0.03858564569666356
1201	28	0.020772234976611514
1201	29	0.01777182464834226
1201	30	0.039743870477160215
1201	31	0.04306414690807259
1201	32	0.0694324297441895
1201	33	0.040423439165884645
1201	34	0.0967038221922536
1201	35	-0.057181997134009066
1201	36	0.03057376878281559
1201	37	0.025908246882671904
1201	38	0.019643345582402105
1201	39	1.2353834959896446
1201	40	0.006534532926607994
1201	41	0.061175129595014255
1201	42	0.0373954046120204
1201	43	0.05900075007062355
1201	44	-0.01000968990994977
1201	45	-7.980386366384096E-4
1201	46	0.05344419132377709
1201	47	-0.037872171012921285
1201	48	-0.00821202465474613
1201	49	0.029361588628461277
1201	50	-0.02412932244256183
1201	51	-0.05408525929772168
1201	52	-0.07649396692080757
1201	53	-0.005894568296227476
1201	54	-0.02429902594550981
1201	55	0.1423667791945178
1201	56	0.06337656809578363
1201	57	0.09902761629405887
1201	58	0.0034634297663629354
1201	59	0.027632411459869388
1201	60	0.03509545180720153
1201	61	0.12159768699282836
1202	1	-0.007357165964585022
1202	2	0.10417167908403258
1202	3	0.011113754534044062
1202	4	0.004099505103532408
1202	5	-0.01944778282271642
1202	6	0.025814417050440852
1202	7	0.01843512515883816
1202	8	-0.14607550373617784
1202	9	-0.14670474442397818
1202	10	0.09334753962252762
1202	11	0.008779209837605606
1202	12	-0.010212095410381039
1202	13	-0.01412581888892106
1202	14	-0.03990075083817857
1202	15	-0.0728878448706638
1202	16	0.0014697519636825973
1202	17	-0.018238315871620614
1202	18	-0.10171535451530644
1202	19	0.021288391747546598
1202	20	-0.06641517423125975
1202	21	0.017488116174249058
1202	22	0.019407750139237123
1202	23	-0.022529048597405676
1202	24	0.02350146816664278
1202	25	0.08417009796109198
1202	26	0.11494763107022266
1202	27	0.08731750601769761
1202	28	-0.13774643811527199
1202	29	-0.03774760387594256
1202	30	-0.022161954440463205
1202	31	-0.013715414035260665
1202	32	-0.059862171097947225
1202	33	0.0293952584638717
1202	34	-0.05040167467035417
1202	35	-0.01982853403453788
1202	36	-0.16860156432728246
1202	37	-0.15546832241894748
1202	38	-0.17825244356946257
1202	39	1.0158818549057713
1202	40	-0.14431092048650385
1202	41	-0.3033201946504889
1202	42	-0.19482645633988938
1202	43	-0.10585174649595785
1202	44	-0.18625109828182218
1202	45	-0.1388059941324542
1202	46	-0.2104702426114713
1202	47	-0.01699020772985449
1202	48	-0.06749971822419099
1202	49	-0.09732943951988915
1202	50	-0.05671320860035678
1202	51	-0.054827750671719855
1202	52	-0.15605458673125128
1202	53	-0.07782564439107631
1202	54	-0.15214665830389862
1202	55	-0.18776041491602058
1202	56	-0.07345456283666607
1202	57	-0.04413652929896017
1202	58	-0.06715979387560367
1202	59	-0.15231983615372968
1202	60	-0.1550106484307534
1202	61	-0.14605589467879554
1203	1	0.16475597342127912
1203	2	0.044045946231030086
1203	3	0.08324203029868471
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2403	7	0.08691893471092271
2403	8	-0.018095477630929224
2403	9	0.02349553048093611
2403	10	0.0024137122189458182
2403	11	0.02393855821237878
2403	12	0.025443912300808336
2403	13	0.02560593856480864
2403	14	0.09320385237607809
2403	15	0.03576473353784593
2403	16	0.01284689033056452
2403	17	-0.02769672681570512
2403	18	0.04607200727983951
2403	19	0.14560824121772953
2403	20	0.10418170913533942
2403	21	0.06373134352818965
2403	22	0.07639156340179198
2403	23	0.0708157624693726
2403	24	-0.0031372627522472385
2403	25	-0.0027638382830659225
2403	26	-0.01911344993107633
2403	27	0.007680011098983641
2403	28	0.0664896041115739
2403	29	-0.03153956830816185
2403	30	0.05487738400672981
2403	31	0.01804954309227469
2403	32	-0.006175732551227497
2403	33	0.005676258444907489
2403	34	0.12517878778665192
2403	35	0.0020203870008046465
2403	36	0.0027423611729915365
2403	37	0.04092790015343439
2403	38	0.03696000581349068
2403	39	1.2290815781868503
2403	40	0.02105914159385236
2403	41	0.04206640789918481
2403	42	0.022187634391919175
2403	43	0.10400030453048004
2403	44	-0.01725219245943066
2403	45	0.10464646191285709
2403	46	0.10151302232545589
2403	47	0.03383999025280815
2403	48	0.02796583497040217
2403	49	0.036203105049089856
2403	50	0.027513730596517405
2403	51	-0.05989708294303142
2403	52	0.012005156850250387
2403	53	0.04107526368563441
2403	54	-0.050803714017902735
2403	55	0.02738771958124886
2403	56	-0.0721220903333375
2403	57	-0.06055991254228843
2403	58	0.018191006721046676
2403	59	-9.36612609201859E-4
2403	60	-0.05238388008060468
2403	61	0.06230619482938238
2404	1	-0.012363292163250605
2404	2	-0.06422904928145101
2404	3	-0.01888685141016566
2404	4	-0.005193830434478741
2404	5	0.012056797191192459
2404	6	-0.02283900032085029
2404	7	0.06362459569711376
2404	8	0.03772360213665138
2404	9	0.0182948909824745
2404	10	0.08001471295440865
2404	11	0.09756092305756425
2404	12	-0.0844831907418353
2404	13	3.260532219329093E-4
2404	14	-0.01945557697840883
2404	15	-0.04795005685459586
2404	16	-0.08177876629731884
2404	17	-0.009564892071161069
2404	18	-0.0268777526987094
2404	19	-0.005010408585619075
2404	20	-0.09024048975873455
2404	21	-0.069763331159443
2404	22	-0.06389704904160709
2404	23	-0.021561591909573963
2404	24	-0.004732656351812636
2404	25	-0.09343851753855148
2404	26	0.027451341194733914
2404	27	-0.03382327125466844
2404	28	-0.0903283253131093
2404	29	-0.09878035875507862
2404	30	-0.0373109705000374
2404	31	-0.14097520080451886
2404	32	0.058330636241578304
2404	33	-0.07847184872765922
2404	34	0.022535932589470065
2404	35	0.0368106118100755
2404	36	0.09578771066560421
2404	37	0.04980544625620098
2404	38	-0.018736705965899603
2404	39	1.2872503747689308
2404	40	-0.0501003579546051
2404	41	-0.04344325267631888
2404	42	-0.09799574996299754
2404	43	0.015813634678892186
2404	44	-0.07522486500563019
2404	45	0.01188021200976408
2404	46	-0.13339496071903767
2404	47	-0.07584279588189702
2404	48	-0.07033882360405386
2404	49	0.0490592870421338
2404	50	0.029979948440875148
2404	51	-0.014269747946002553
2404	52	-0.10806298368017053
2404	53	-0.08401038342939415
2404	54	0.07266768141718671
2404	55	-0.09375155991436657
2404	56	0.009913911837436729
2404	57	-0.15794723027370594
2404	58	-0.09106768213948868
2404	59	-0.14355356937600305
2404	60	-0.10680830003747843
2404	61	-0.042558980372660926
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	8.0
17	43.0
18	117.0
19	162.0
20	194.0
21	230.0
22	347.0
23	524.0
24	720.0
25	946.0
26	1260.0
27	1631.0
28	2278.0
29	2670.0
30	3498.0
31	4315.0
32	5676.0
33	7092.0
34	9030.0
35	11468.0
36	16933.0
37	24153.0
38	59197.0
39	409822.0
40	430371.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.751356170386376	11.218160846592827	33.57580702841284	30.454675954607957
2	27.853361777684622	16.235852982877965	29.344271711407398	26.56651352803001
3	28.30898019009051	19.568644635508747	21.37405118441399	30.748323989986755
4	26.908636677294407	19.513239345814632	21.215894266559886	32.36222971033107
5	27.420581554068008	19.585064748636274	20.64975294277641	32.34460075451931
6	29.89880979364048	21.757657262877952	22.536454162196467	25.807078781285103
7	27.538846663342348	21.303233150495878	22.150732609035092	29.00718757712668
8	25.558057188332654	23.079627474979475	22.62318862479034	28.739126711897526
9	26.44796687771045	23.09906969481759	22.59669482262752	27.856268604844438
10	26.83650906380171	21.214584686985297	22.53957700579741	29.409329243415584
11	25.763056760200868	22.149120818789445	22.37054050378519	29.717281917224497
12	27.921848320464193	21.714138926245486	22.04475740038381	28.319255352906513
13	25.97843223177544	20.45492779683384	22.313422686955075	31.253217284435646
14	26.462170779250215	21.653193107581963	22.170477039544267	29.71415907362356
15	27.030125367060045	21.1940343613533	21.871792159647825	29.90404811193883
16	26.616801905941966	21.73247304028972	21.78122969522054	29.869495358547777
17	26.705954053904314	20.6820894845797	21.88478721850335	30.727169243012636
18	28.417876768562035	21.488991976306686	21.59305318404126	28.500078071090023
19	26.53107481225162	20.713519394369815	22.8176108231715	29.937794970207065
20	26.693865627061957	21.177110563774008	21.741136412860072	30.387887396303963
21	27.08230707626286	21.57129401572503	22.67436296508963	28.67203594292248
22	27.481426635841178	21.17237592992742	21.888917431007823	29.45728000322358
23	26.56713861899797	22.260032135068023	21.873806897454884	29.299022348479127
24	27.256884107244495	23.13825634516488	21.569782962369736	28.035076585220892
25	26.54689050403703	21.33254758558858	21.97202536554899	30.1485365448254
26	26.752595234137715	21.757757999768305	22.83161325093056	28.65803351516342
27	27.202687660234616	22.589341029631758	21.52948820622856	28.678483103905066
28	26.655283398056785	22.291462044858136	21.74103567596972	29.31221888111536
29	26.82724126988924	23.589154666384605	21.662259427713725	27.921344636012428
30	28.645441403869302	23.508061469650492	21.14215486282154	26.704342263658663
31	26.94389458891793	23.024423659066066	22.474198763958356	27.55748298805764
32	27.300603917657668	23.469378503754967	21.125130328351894	28.10488725023547
33	27.70647284888963	25.220185658088923	20.93866634430862	26.134675148712834
34	27.297783284727785	23.97769685247586	20.81677470698157	27.907745155814784
35	27.611578698177162	24.35566166508006	20.527559094778304	27.50520054196447
36	28.14044737253006	25.2125296544221	20.092980149795757	26.55404282325209
37	27.719568644635505	26.023360884872844	20.032840226255054	26.22423024423659
38	27.81788784961997	26.59806484433632	19.71894407591532	25.86510323012839
39	28.75051592693747	25.85862198384174	19.23313243805498	26.157729651165813
40	28.976059878007625	25.961407697305795	19.225333313185956	25.837199111500624
41	28.278255438532867	27.20389650291885	18.88746178294222	25.63038627560606
42	28.378287170653326	27.972418239421366	18.658990515621774	24.99030407430353
43	27.93977948694702	27.76721719377245	18.83447417861658	25.458529140663956
44	28.04877680230889	26.94409606269864	19.54607957206969	25.46104756292278
45	28.26969280285287	27.407787968993187	18.22048283191546	26.102036396238486
46	27.67242377995034	27.333444143912722	19.261094909261246	25.733037166875693
47	28.732175866463177	27.436699456524476	18.10493761868065	25.726187058331696
48	28.793222422017056	27.994681092189367	17.698967950558334	25.513128535235246
49	28.38987191304392	27.888101462195962	17.859441816890552	25.862584807869567
50	28.27533406871263	27.917012949727255	17.709948271606805	26.097704709953305
51	28.231312047628403	28.20854551040864	17.677309519132454	25.882832922830506
52	27.54680487768023	28.196658557346993	17.605181905639753	26.65135465933302
53	27.57339941673341	28.19212539728111	17.49789711741388	26.736578068571603
54	27.357318786926367	28.37969748711827	17.353238942867073	26.90974478308829
55	26.74473775669019	28.426640878022734	17.368047265748952	27.46057409953812
56	26.494809531724567	28.45253025884344	17.22913109395226	27.823529115479733
57	26.260294050982942	28.65339961820719	17.063116698650628	28.023189632159244
58	25.519877906888894	28.769146305222705	17.087897973677453	28.62307781421095
59	25.549796763323712	28.718677123155885	16.831321113948533	28.900204999571866
60	25.142618252517163	28.950573444748333	16.796667623667126	29.110140679067374
61	24.907699824214127	29.02300326891209	16.60305132040879	29.46624558646499
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	5.5
2	7.5
3	6.0
4	5.5
5	5.0
6	5.5
7	7.0
8	8.0
9	7.5
10	7.0
11	12.0
12	16.5
13	16.0
14	28.0
15	40.0
16	44.0
17	48.5
18	49.0
19	75.0
20	125.5
21	150.0
22	194.5
23	239.0
24	310.5
25	478.0
26	574.0
27	752.0
28	930.0
29	1185.0
30	1824.0
31	2208.0
32	2792.0
33	3376.0
34	4133.0
35	6005.5
36	7121.0
37	8470.5
38	9820.0
39	11208.0
40	14538.0
41	16480.0
42	18626.5
43	23935.0
44	27097.0
45	30670.0
46	34243.0
47	38223.5
48	46781.5
49	51359.0
50	56113.5
51	60868.0
52	66347.0
53	75852.5
54	79879.0
55	82422.5
56	84966.0
57	85878.5
58	84906.0
59	83021.0
60	78171.5
61	71017.5
62	68713.0
63	57066.0
64	45419.0
65	39396.0
66	28590.5
67	23808.0
68	20015.5
69	16223.0
70	13590.5
71	8904.0
72	6850.0
73	5550.5
74	4251.0
75	3452.5
76	2111.0
77	1568.0
78	1225.0
79	882.0
80	687.0
81	377.5
82	263.0
83	201.0
84	116.5
85	94.0
86	73.5
87	53.0
88	41.0
89	19.0
90	9.0
91	9.5
92	10.0
93	7.5
94	6.5
95	8.0
96	7.5
97	7.0
98	6.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03404906893929091
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.1051139082387667
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	992685.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.12391802895844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.7305373507493	66.28694793183121
2	13.503937122136522	21.639767020845515
3	2.6316239497600864	6.32568064900863
4	0.6335581246167108	2.0305263697347984
5	0.22021816598506477	0.8822371139937448
6	0.09309287848535801	0.4475379698904368
7	0.06787348185547273	0.3806802507569527
8	0.04320050688139514	0.2769115097739487
9	0.02151408635595887	0.15514136024875036
>10	0.05417404346776487	0.5745730736390309
>50	1.3514485321533456E-4	0.006388717733226041
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	1.3514485321533456E-4	0.9936080325437653
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACGAAATCTGGGGGGGGGGGGGGG	9176	0.9243617058785012	TruSeq Adapter, Index 2 (97% over 36bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.039503971551902	0.0	0.0	0.0	0.0
2	1.0437349209467253	1.007368903529317E-4	0.0	0.0	3.022106710587951E-4
3	1.0446415529599016	1.007368903529317E-4	0.0	0.0	3.022106710587951E-4
4	1.045548184973078	1.007368903529317E-4	0.0	0.0	3.022106710587951E-4
5	1.0478651334511955	1.007368903529317E-4	0.0	0.0	3.022106710587951E-4
6	1.051592398394254	1.007368903529317E-4	0.0	0.0	3.022106710587951E-4
7	1.0555211371180182	1.007368903529317E-4	0.0	0.0	3.022106710587951E-4
8	1.089872416728368	1.007368903529317E-4	0.0	0.0	3.022106710587951E-4
9	1.094506313684603	2.014737807058634E-4	0.0	0.0	3.022106710587951E-4
10	1.104479265829543	2.014737807058634E-4	0.0	0.0	3.022106710587951E-4
11	1.113545585961307	2.014737807058634E-4	0.0	0.0	3.022106710587951E-4
12	1.1339951747029522	2.014737807058634E-4	0.0	1.007368903529317E-4	3.022106710587951E-4
13	1.1604889768657731	2.014737807058634E-4	0.0	1.007368903529317E-4	3.022106710587951E-4
14	1.1967542573928285	2.014737807058634E-4	0.0	1.007368903529317E-4	3.022106710587951E-4
15	1.246316807446471	2.014737807058634E-4	0.0	1.007368903529317E-4	3.022106710587951E-4
16	1.3098817852591709	2.014737807058634E-4	0.0	1.007368903529317E-4	3.022106710587951E-4
17	1.3970191954144568	2.014737807058634E-4	0.0	1.007368903529317E-4	4.029475614117268E-4
18	1.5216307287810333	2.014737807058634E-4	0.0	1.007368903529317E-4	4.029475614117268E-4
19	1.7015468149513693	2.014737807058634E-4	0.0	1.007368903529317E-4	4.029475614117268E-4
20	1.9560081999828747	2.014737807058634E-4	0.0	1.007368903529317E-4	4.029475614117268E-4
21	2.2925701506520197	2.014737807058634E-4	0.0	1.007368903529317E-4	4.029475614117268E-4
22	2.7350065730820954	2.014737807058634E-4	0.0	1.007368903529317E-4	4.029475614117268E-4
23	3.256521454439223	2.014737807058634E-4	0.0	1.007368903529317E-4	4.029475614117268E-4
24	3.916952507593043	2.014737807058634E-4	0.0	1.007368903529317E-4	4.029475614117268E-4
25	4.67519908127956	2.014737807058634E-4	0.0	1.007368903529317E-4	4.029475614117268E-4
26	5.561985927056417	2.014737807058634E-4	0.0	1.007368903529317E-4	4.029475614117268E-4
27	6.574794622664793	2.014737807058634E-4	0.0	1.007368903529317E-4	4.029475614117268E-4
28	7.664767776283514	2.014737807058634E-4	0.0	1.007368903529317E-4	4.029475614117268E-4
29	8.876733304119636	2.014737807058634E-4	0.0	1.007368903529317E-4	5.036844517646585E-4
30	10.232853322050802	2.014737807058634E-4	0.0	1.007368903529317E-4	6.044213421175902E-4
31	11.61717966928079	2.014737807058634E-4	0.0	1.007368903529317E-4	6.044213421175902E-4
32	13.14938777154888	2.014737807058634E-4	0.0	1.007368903529317E-4	6.044213421175902E-4
33	14.797846245284255	2.014737807058634E-4	0.0	1.007368903529317E-4	6.044213421175902E-4
34	16.51158222396833	2.014737807058634E-4	0.0	1.007368903529317E-4	6.044213421175902E-4
35	18.291603076504632	2.014737807058634E-4	0.0	1.007368903529317E-4	6.044213421175902E-4
36	20.192306723683746	2.014737807058634E-4	0.0	1.007368903529317E-4	6.044213421175902E-4
37	22.060472355278865	2.014737807058634E-4	0.0	1.007368903529317E-4	6.044213421175902E-4
38	24.06977037025844	3.022106710587951E-4	0.0	1.007368903529317E-4	6.044213421175902E-4
39	26.09538776147519	3.022106710587951E-4	0.0	1.007368903529317E-4	6.044213421175902E-4
40	28.226174466220403	3.022106710587951E-4	0.0	1.007368903529317E-4	6.044213421175902E-4
41	30.36139359414114	3.022106710587951E-4	0.0	1.007368903529317E-4	6.044213421175902E-4
42	32.5881825553927	3.022106710587951E-4	0.0	1.007368903529317E-4	6.044213421175902E-4
43	34.79391750656049	3.022106710587951E-4	0.0	1.007368903529317E-4	6.044213421175902E-4
44	36.9777925525217	3.022106710587951E-4	0.0	1.007368903529317E-4	6.044213421175902E-4
45	39.17284939331208	3.022106710587951E-4	0.0	1.007368903529317E-4	6.044213421175902E-4
46	41.30766557367141	3.022106710587951E-4	0.0	1.007368903529317E-4	7.051582324705219E-4
47	43.41498058296438	3.022106710587951E-4	0.0	1.007368903529317E-4	7.051582324705219E-4
48	45.550904869117595	3.022106710587951E-4	0.0	1.007368903529317E-4	7.051582324705219E-4
49	47.59012174053199	3.022106710587951E-4	0.0	1.007368903529317E-4	7.051582324705219E-4
50	49.61735092199439	3.022106710587951E-4	0.0	1.007368903529317E-4	7.051582324705219E-4
>>END_MODULE